ARHGEF10 cDNA ORF clone, Maylandia zebra(Zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF10
Entrez Gene ID	101483247
Full Name	Rho guanine nucleotide exchange factor 10
Gene Type	protein-coding
Organism	Maylandia zebra(Zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023153848.2	XP_023009616.2	rho guanine nucleotide exchange factor 10 isoform X2
XM_004566501.4	XP_004566558.1	rho guanine nucleotide exchange factor 10 isoform X1
XM_014410611.2	XP_014266097.1	rho guanine nucleotide exchange factor 10 isoform X3
XM_014410611.3	XP_014266097.2	rho guanine nucleotide exchange factor 10 isoform X3
XM_004566501.3	XP_004566558.1	rho guanine nucleotide exchange factor 10 isoform X1
XM_023153848.1	XP_023009616.1	rho guanine nucleotide exchange factor 10 isoform X2
XM_004566501.5	XP_004566558.2	rho guanine nucleotide exchange factor 10 isoform X1
XM_014410611.1	XP_014266097.1	rho guanine nucleotide exchange factor 10 isoform X2

Pathways from BioSystems
Gene Ontology

The following ARHGEF10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa13328	XM_004566501.4 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa13329	XM_014410611.2 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa310598	XM_023153848.1 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa366280	XM_004566501.5 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa366281	XM_023153848.2 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa366282	XM_014410611.3 Latest version!	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMa13328	XM_004566501.3	Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa13329	XM_014410611.1	Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF10 ( XM_004566501.4 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_004566558.1 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa13328
Clone ID Related Accession (Same CDS sequence)	XM_004566501.4 , XM_004566501.3
Accession Version	XM_004566501.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004566501.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAGATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004566558.1
CDS	263..4540
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETRPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHLSQDLTRLKEHYEKKMKDLMANTVGTVELQQLKQKHEQKMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_004566501.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004566501.4

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAGATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_004566501.3 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_004566558.1 Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa13328
Clone ID Related Accession (Same CDS sequence)	XM_004566501.4 , XM_004566501.3
Accession Version	XM_004566501.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004566501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAGATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004566558.1
CDS	259..4536
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETRPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHLSQDLTRLKEHYEKKMKDLMANTVGTVELQQLKQKHEQKMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_004566501.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004566501.3

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAGATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_014410611.2 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_014266097.1 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

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CloneID	OMa13329
Clone ID Related Accession (Same CDS sequence)	XM_014410611.2 , XM_014410611.1
Accession Version	XM_014410611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014410611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAG 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014266097.1
CDS	263..4423
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETRPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_014410611.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014410611.2

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAG 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_014410611.1 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_014266097.1 Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

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CloneID	OMa13329
Clone ID Related Accession (Same CDS sequence)	XM_014410611.2 , XM_014410611.1
Accession Version	XM_014410611.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAG 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014266097.1
CDS	259..4419
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETRPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_014410611.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor (GEF) 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014410611.1

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GACGCTCGTG CTCCACACGT CACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAG 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_023153848.1 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_023009616.1 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

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CloneID	OMa310598
Clone ID Related Accession (Same CDS sequence)	XM_023153848.1
Accession Version	XM_023153848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGTC AGTGAAGGCC AGAAAGTATC TGTCAGCGAC 
GCTCGTGCTC CACACGTCAC CCCCGGTTCC TCCTCCCAGG GCAAAGTCAA CCCCTACTCT 
GTGATTGACA TCACTTCCTT CCAGCAGCAG CAGCTTGACC AGCAGCACAG ACCCGCCTCT 
CCCACCCCCT CGCCCACGGA GCCCAAAGAA CGAGAGGCAG ACGGCCAGGA TCCTCCCGGC 
AGCCCTGCTG GCATCGCTTC GGGATACTCG GTGCCTGTCC CGTGTGGCTA TGCGACCCCC 
TCTGGAGTAC CGCTCATCAC CCCAGCTTAC ACCACACCTG TCATCATTCG ACACCTCTCC 
GTGGATGAAG ATGTGATGCT GGTTGGGACT GGGAACGCCT CAGCTGACAG TGTTTTCAGT 
GAAGAGTATC CACCTATCAG AGAAGAGGAT GCTTTGGCAA AATGGGCAGC AGATCCTGCC 
AACACCGCAT GGATGGAAAA TCCACATGAG GTGATTTATG ATGATGTGCC CAGAGAAAAC 
TCCGACTCCA ACACAGATCC AGATGAGATG ATCTACGACG ATGTGGAGCT CGGCGAGGAG 
GGCGGCTGCA ACAGCTCCCT CGACAACGGC TGGAGCTCCA GCGAGTTTGA AAGCTACGAC 
GAAGCCAGCG ACGGGGAAGG TCGGCCTGAG AACGGCCTGC CCCACGCCTT CATGAGAGGG 
AAGCCGCCCC AGAGGAAGAC TCATCTCTCT CAAGATCTGA CACGCTTGAA AGAACACTAT 
GAGAAAAAGA TGAAAGATCT AATGGCAAAC ACAGTGGGAA CGGTAGAGCT GCAGCAGTTA 
AAACAGAAAC ACGAGCAGAA GATGCAAAAG CTGGTGAAGG CTGCCAAGGA AGGAACCAAA 
GATGGACTGG AGAAAACCAA AGCTGCAGTG AAGAAGGGAC GCTCCTTCAT CAAAACCAAG 
TCTTTCTGTC AGGAGAAGTC GGCCTGTTTT GAGGATGAGG AGTCTGGACT CTTCATCGAG 
GTGGACTGTT TCAATGTTGA GCCAGTACTG TGTCCAGCAC CGGAGGCTCT GTCACAGCAG 
CAGCTGGTCA GAAGATGCAT CCTGGGATCT ATTTTGGAGA GCGAGAAGAA TTATCTCGAT 
TCTCTCAAGA GGATTTTAGA GCAATACGAG AAGCCCCTGT CGCAGATCGA GCCCAGACTG 
CTGAGTGACA GGAAACTGAA GATGACTTTC TATCGCGTGC GTGAAATCCT GCAGTGCCAC 
TTCCTGTTCC AGATCGCCCT GGCGAGCCGC GTGGCGGAGT GGGACAGCCT GGAGATGATT 
GGGGATGTCT TTGTGGCGTC GTTCTCTAAG TCCATGGTGC TGGACGCCTA TCGCGAGTAT 
GTGAATAACT TCAGCACTGC GATGGCAGTC GTGAGGAAGA CGTGCGCCTC CAAACCAGCA 
TTCCTGGAAT TCCTCAAGCA TCGTCAGGAA ACCAGCAGTG ACCGAATGAC TCTGTACGGC 
CTGATGATGA AGCCTATCCA GAGATTCCCT CAGTTCATTT TGCTGCTGCA GGACATGTTG 
AAGAACACAC CAGTGGGTCA CCCGGACCGC CTGCCCCTGC AGATGGCCCT GACCGAACTG 
GAGACTCTGG CTGAGAAGCT CAATGAAAAG AAGAGAGAAG CCGACCAGCG CTGCGAGATC 
CGTCACATCG CAAAGGCCAT GAACGAGCGC TACCTCAACA AGCTTCTAAG TAACGGCAGC 
CGCTACTTGA TCCGCTCAGA CGACATGGTG GAAACGGTCT ACAACGAGCG TGGGGAAATC 
ATCAAAACCA AGGAGCGACG CCTGTTCCTG CTCAACGATG TGCTCATGTG TGCCACGCCC 
AACATCCGAT TTCAGGATGG CAGCGGCAGT GGCAGCGGAA GCGTAACACT TGACCAGAAG 
TTCCTTCTGA AGTGGAGCGT GCCTCTGAGC TTCGTGGAGG TGCTTGAGTT CGGGTCCAGC 
GAAGACATGG CCGACACCGG CCGCTTCCCT CCTCCTCATT CGGGCGAGAA AGTGGTGATC 
AGCGCCAAGC CAAATAAACT GTACATGGGC CCCGGTCAGC TGTACCAAGA CCTGCAGAAC 
CTAATCCATG ATCTCAGCCT GGTCAACCAG ATCTCCACCA TGATTGGCAG CCTCAAGGGA 
AACTACCAGA ATGTAAATCC CACCGTGGCC CAAGACTGGG TATCAGGACT CCAGAGGCTG 
ATACTGAAGA AGGAGGAGGA GATCAGGGCA GCTGACCGGT GTAGGATCCA GCTCCAGCTG 
CCTGGCAAAC AAGACAAGGC GGGGAAGCCG ACCTACTTCA GTGCAGTGTT CAATACCCTC 
AGCCCAGCCA TCAAACAGTC ATGGATCAGC AACTTGCAGA TGGCGAAGCT GGCTCTCGAG 
GAGGACAATG TGCAGGGCTG GTTCTTTGCA GAGGATGATG GAAATCAAAT GAAGAAGCAG 
AAACACCCCC TGCTGCTTCG ACAAATGCCC GTGGTCATGT CCAAACTGCA GGAGTTTAAG 
GTGGAGTGTG CTGTCCACAA CCCGGAGCCC CACATCAACA CAGACAGCAC AGCAGACACT 
CCAGTGGTGG GACATGGATG TGTCTGGGTT GCGAGTTGCA CCAACCAGAT GGGACAGATT 
GCCATAGTGG CCATGCAGAA CTCCAGCCCT AAGGTGACCG AGTGTTTCAA CGTGGAGTCC 
CGGATTCTCT GCATGGCGTA CGTCCCAGCT GAGCAACGCG AGGAGAACGG CGAAAACGTC 
CCTGACGATA ACCAAGTGTC CTCGGCGAAG GCCAGCGATG CCCCCAGCGT CTGCCTGGGC 
ATGGAGGAGG GGAGCATCAT TGTGTACAAG AGCAGCCAGC GGTCTAAGAA GGTGCGTCTA 
CAGCATTTCT TCACCCCAGA CAAGTCCACT GTCACCAGCC TGGTGTACAG GAAGCAGTGC 
CTGTACGCTG GGCTGGTCAG CGGCTCCGTG GCAATCTACT CCAGATCAAA AGATGGATCG 
TGGAACAGCG ACAAGCCCAA GTTACTGAAG CTGGGAGTGC TCCCTGTGAA GGCCATGCTA 
GCTGTGGAGG AGCACCTCTG GGCCTCGTCG GGTGGGCAGG TCTTCATCAT CAGCACCCAC 
GCACACTCTG TAGAGAGGCA GTTAGAGGCC CACCAAGATG AGGGCATGGT GGTGTCACAC 
ATGGTGGTGG CCGGGGTGGG CATATGGATT GCCTTCAGCT CTGGTTCCAC GCTGCGCCTC 
TTCCACACCG AAACCCTAGA GCACCTGCAG GACATCAACA TCGCCACACC TGTCCACAGT 
ATACTTCCAG GGAACCAGAG AGTGTCGGTG AGCAGTCTGC TGGTTTGTCA TGGTCTGCTG 
CTGGTAGGGA CCAACCTGGG AGTGACTGTA GCGCTCTCTG TCCCCAGGCT GCAGGGCATC 
CCTAAAGTAA CAGGTCGGGG CATGGTGTCC TTACATGCTC ACAGCGGACC GGTGAAGTTC 
CTCACCGCTG CCGCTGCAGT GTGTAACCAG CCGTCAGGCC CACTGCAGCC TGCAGAGTCA 
AAGGATGTGG AGAGCACCCA GCCTCCATCA GACTCAGCCC CGACCCCCTC GCAGGGACGA 
GGGGTGTGGC TGGGAGAGGC GGGCTGCACT GCCATGGCTC TCCAGGACTC CCTGTCCTCC 
TCGTCCTGTG GCTCGCTGGC TCCATCCCAG GGTTCGTTGG AGCACGGGCC TGAGGACGGG 
GCCCTTTATG ATGTGCTCCA CGACCCGGCC CATCACCAGA GAGGCCGCCG CTCAAGTAAG 
GTGGATGTCA GCTCTCTCTT GGTGGTCTCT GGAGGTCTGG GACACCGCAG GGTCAACAAG 
AAGACCAAGC AGTCTCGCCA TGAGGACACC ACCTCTACCA TCATGATCTG GCAGATCCCC 
TTACTCAACA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009616.1
CDS	263..4537
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETRPAATVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHLSQDLTRLKEHYEKKMKDLMANTVGTVELQQLKQKHEQKMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_023153848.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153848.1

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACGTCCTGC AGCAACAGTC AGTGAAGGCC AGAAAGTATC TGTCAGCGAC 
GCTCGTGCTC CACACGTCAC CCCCGGTTCC TCCTCCCAGG GCAAAGTCAA CCCCTACTCT 
GTGATTGACA TCACTTCCTT CCAGCAGCAG CAGCTTGACC AGCAGCACAG ACCCGCCTCT 
CCCACCCCCT CGCCCACGGA GCCCAAAGAA CGAGAGGCAG ACGGCCAGGA TCCTCCCGGC 
AGCCCTGCTG GCATCGCTTC GGGATACTCG GTGCCTGTCC CGTGTGGCTA TGCGACCCCC 
TCTGGAGTAC CGCTCATCAC CCCAGCTTAC ACCACACCTG TCATCATTCG ACACCTCTCC 
GTGGATGAAG ATGTGATGCT GGTTGGGACT GGGAACGCCT CAGCTGACAG TGTTTTCAGT 
GAAGAGTATC CACCTATCAG AGAAGAGGAT GCTTTGGCAA AATGGGCAGC AGATCCTGCC 
AACACCGCAT GGATGGAAAA TCCACATGAG GTGATTTATG ATGATGTGCC CAGAGAAAAC 
TCCGACTCCA ACACAGATCC AGATGAGATG ATCTACGACG ATGTGGAGCT CGGCGAGGAG 
GGCGGCTGCA ACAGCTCCCT CGACAACGGC TGGAGCTCCA GCGAGTTTGA AAGCTACGAC 
GAAGCCAGCG ACGGGGAAGG TCGGCCTGAG AACGGCCTGC CCCACGCCTT CATGAGAGGG 
AAGCCGCCCC AGAGGAAGAC TCATCTCTCT CAAGATCTGA CACGCTTGAA AGAACACTAT 
GAGAAAAAGA TGAAAGATCT AATGGCAAAC ACAGTGGGAA CGGTAGAGCT GCAGCAGTTA 
AAACAGAAAC ACGAGCAGAA GATGCAAAAG CTGGTGAAGG CTGCCAAGGA AGGAACCAAA 
GATGGACTGG AGAAAACCAA AGCTGCAGTG AAGAAGGGAC GCTCCTTCAT CAAAACCAAG 
TCTTTCTGTC AGGAGAAGTC GGCCTGTTTT GAGGATGAGG AGTCTGGACT CTTCATCGAG 
GTGGACTGTT TCAATGTTGA GCCAGTACTG TGTCCAGCAC CGGAGGCTCT GTCACAGCAG 
CAGCTGGTCA GAAGATGCAT CCTGGGATCT ATTTTGGAGA GCGAGAAGAA TTATCTCGAT 
TCTCTCAAGA GGATTTTAGA GCAATACGAG AAGCCCCTGT CGCAGATCGA GCCCAGACTG 
CTGAGTGACA GGAAACTGAA GATGACTTTC TATCGCGTGC GTGAAATCCT GCAGTGCCAC 
TTCCTGTTCC AGATCGCCCT GGCGAGCCGC GTGGCGGAGT GGGACAGCCT GGAGATGATT 
GGGGATGTCT TTGTGGCGTC GTTCTCTAAG TCCATGGTGC TGGACGCCTA TCGCGAGTAT 
GTGAATAACT TCAGCACTGC GATGGCAGTC GTGAGGAAGA CGTGCGCCTC CAAACCAGCA 
TTCCTGGAAT TCCTCAAGCA TCGTCAGGAA ACCAGCAGTG ACCGAATGAC TCTGTACGGC 
CTGATGATGA AGCCTATCCA GAGATTCCCT CAGTTCATTT TGCTGCTGCA GGACATGTTG 
AAGAACACAC CAGTGGGTCA CCCGGACCGC CTGCCCCTGC AGATGGCCCT GACCGAACTG 
GAGACTCTGG CTGAGAAGCT CAATGAAAAG AAGAGAGAAG CCGACCAGCG CTGCGAGATC 
CGTCACATCG CAAAGGCCAT GAACGAGCGC TACCTCAACA AGCTTCTAAG TAACGGCAGC 
CGCTACTTGA TCCGCTCAGA CGACATGGTG GAAACGGTCT ACAACGAGCG TGGGGAAATC 
ATCAAAACCA AGGAGCGACG CCTGTTCCTG CTCAACGATG TGCTCATGTG TGCCACGCCC 
AACATCCGAT TTCAGGATGG CAGCGGCAGT GGCAGCGGAA GCGTAACACT TGACCAGAAG 
TTCCTTCTGA AGTGGAGCGT GCCTCTGAGC TTCGTGGAGG TGCTTGAGTT CGGGTCCAGC 
GAAGACATGG CCGACACCGG CCGCTTCCCT CCTCCTCATT CGGGCGAGAA AGTGGTGATC 
AGCGCCAAGC CAAATAAACT GTACATGGGC CCCGGTCAGC TGTACCAAGA CCTGCAGAAC 
CTAATCCATG ATCTCAGCCT GGTCAACCAG ATCTCCACCA TGATTGGCAG CCTCAAGGGA 
AACTACCAGA ATGTAAATCC CACCGTGGCC CAAGACTGGG TATCAGGACT CCAGAGGCTG 
ATACTGAAGA AGGAGGAGGA GATCAGGGCA GCTGACCGGT GTAGGATCCA GCTCCAGCTG 
CCTGGCAAAC AAGACAAGGC GGGGAAGCCG ACCTACTTCA GTGCAGTGTT CAATACCCTC 
AGCCCAGCCA TCAAACAGTC ATGGATCAGC AACTTGCAGA TGGCGAAGCT GGCTCTCGAG 
GAGGACAATG TGCAGGGCTG GTTCTTTGCA GAGGATGATG GAAATCAAAT GAAGAAGCAG 
AAACACCCCC TGCTGCTTCG ACAAATGCCC GTGGTCATGT CCAAACTGCA GGAGTTTAAG 
GTGGAGTGTG CTGTCCACAA CCCGGAGCCC CACATCAACA CAGACAGCAC AGCAGACACT 
CCAGTGGTGG GACATGGATG TGTCTGGGTT GCGAGTTGCA CCAACCAGAT GGGACAGATT 
GCCATAGTGG CCATGCAGAA CTCCAGCCCT AAGGTGACCG AGTGTTTCAA CGTGGAGTCC 
CGGATTCTCT GCATGGCGTA CGTCCCAGCT GAGCAACGCG AGGAGAACGG CGAAAACGTC 
CCTGACGATA ACCAAGTGTC CTCGGCGAAG GCCAGCGATG CCCCCAGCGT CTGCCTGGGC 
ATGGAGGAGG GGAGCATCAT TGTGTACAAG AGCAGCCAGC GGTCTAAGAA GGTGCGTCTA 
CAGCATTTCT TCACCCCAGA CAAGTCCACT GTCACCAGCC TGGTGTACAG GAAGCAGTGC 
CTGTACGCTG GGCTGGTCAG CGGCTCCGTG GCAATCTACT CCAGATCAAA AGATGGATCG 
TGGAACAGCG ACAAGCCCAA GTTACTGAAG CTGGGAGTGC TCCCTGTGAA GGCCATGCTA 
GCTGTGGAGG AGCACCTCTG GGCCTCGTCG GGTGGGCAGG TCTTCATCAT CAGCACCCAC 
GCACACTCTG TAGAGAGGCA GTTAGAGGCC CACCAAGATG AGGGCATGGT GGTGTCACAC 
ATGGTGGTGG CCGGGGTGGG CATATGGATT GCCTTCAGCT CTGGTTCCAC GCTGCGCCTC 
TTCCACACCG AAACCCTAGA GCACCTGCAG GACATCAACA TCGCCACACC TGTCCACAGT 
ATACTTCCAG GGAACCAGAG AGTGTCGGTG AGCAGTCTGC TGGTTTGTCA TGGTCTGCTG 
CTGGTAGGGA CCAACCTGGG AGTGACTGTA GCGCTCTCTG TCCCCAGGCT GCAGGGCATC 
CCTAAAGTAA CAGGTCGGGG CATGGTGTCC TTACATGCTC ACAGCGGACC GGTGAAGTTC 
CTCACCGCTG CCGCTGCAGT GTGTAACCAG CCGTCAGGCC CACTGCAGCC TGCAGAGTCA 
AAGGATGTGG AGAGCACCCA GCCTCCATCA GACTCAGCCC CGACCCCCTC GCAGGGACGA 
GGGGTGTGGC TGGGAGAGGC GGGCTGCACT GCCATGGCTC TCCAGGACTC CCTGTCCTCC 
TCGTCCTGTG GCTCGCTGGC TCCATCCCAG GGTTCGTTGG AGCACGGGCC TGAGGACGGG 
GCCCTTTATG ATGTGCTCCA CGACCCGGCC CATCACCAGA GAGGCCGCCG CTCAAGTAAG 
GTGGATGTCA GCTCTCTCTT GGTGGTCTCT GGAGGTCTGG GACACCGCAG GGTCAACAAG 
AAGACCAAGC AGTCTCGCCA TGAGGACACC ACCTCTACCA TCATGATCTG GCAGATCCCC 
TTACTCAACA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_004566501.5 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_004566558.2 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa366280
Clone ID Related Accession (Same CDS sequence)	XM_004566501.5
Accession Version	XM_004566501.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036798.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004566501.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GATGCTCGTG CTCCACACGT TACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAAATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004566558.2
CDS	262..4539
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETHPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHLSQDLTRLKEHYEKKMKDLMANTVGTVELQQLKQKHEQKMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_004566501.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004566501.5

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GATGCTCGTG CTCCACACGT TACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATCTC TCTCAAGATC TGACACGCTT GAAAGAACAC 
TATGAGAAAA AGATGAAAGA TCTAATGGCA AACACAGTGG GAACGGTAGA GCTGCAGCAG 
TTAAAACAGA AACACGAGCA GAAGATGCAA AAGCTGGTGA AGGCTGCCAA GGAAGGAACC 
AAAGATGGAC TGGAGAAAAC CAAAGCTGCA GTGAAGAAGG GACGCTCCTT CATCAAAACC 
AAGTCTTTCT GTCAGGAGAA GTCGGCCTGT TTTGAGGATG AGGAGTCTGG ACTCTTCATC 
GAGGTGGACT GTTTCAATGT TGAGCCAGTA CTGTGTCCAG CACCGGAGGC TCTGTCACAG 
CAGCAGCTGG TCAGAAGATG CATCCTGGGA TCTATTTTGG AGAGCGAGAA GAATTATCTC 
GATTCTCTCA AGAGGATTTT AGAGCAATAC GAGAAGCCCC TGTCGCAGAT CGAGCCCAGA 
CTGCTGAGTG ACAGGAAACT GAAGATGACT TTCTATCGCG TGCGTGAAAT CCTGCAGTGC 
CACTTCCTGT TCCAGATCGC CCTGGCGAGC CGCGTGGCGG AGTGGGACAG CCTGGAGATG 
ATTGGGGATG TCTTTGTGGC GTCGTTCTCT AAGTCCATGG TGCTGGACGC CTATCGCGAG 
TATGTGAATA ACTTCAGCAC TGCGATGGCA GTCGTGAGGA AGACGTGCGC CTCCAAACCA 
GCATTCCTGG AATTCCTCAA GCATCGTCAG GAAACCAGCA GTGACCGAAT GACTCTGTAC 
GGCCTGATGA TGAAGCCTAT CCAGAGATTC CCTCAGTTCA TTTTGCTGCT GCAGGACATG 
TTGAAGAACA CACCAGTGGG TCACCCGGAC CGCCTGCCCC TGCAGATGGC CCTGACCGAA 
CTGGAGACTC TGGCTGAGAA GCTCAATGAA AAGAAGAGAG AAGCCGACCA GCGCTGCGAG 
ATCCGTCACA TCGCAAAGGC CATGAACGAG CGCTACCTCA ACAAGCTTCT AAGTAACGGC 
AGCCGCTACT TGATCCGCTC AGACGACATG GTGGAAACGG TCTACAACGA GCGTGGGGAA 
ATCATCAAAA CCAAGGAGCG ACGCCTGTTC CTGCTCAACG ATGTGCTCAT GTGTGCCACG 
CCCAACATCC GATTTCAGGA TGGCAGCGGC AGTGGCAGCG GAAGCGTAAC ACTTGACCAG 
AAGTTCCTTC TGAAGTGGAG CGTGCCTCTG AGCTTCGTGG AGGTGCTTGA GTTCGGGTCC 
AGCGAAGACA TGGCCGACAC CGGCCGCTTC CCTCCTCCTC ATTCGGGCGA GAAAGTGGTG 
ATCAGCGCCA AGCCAAATAA ACTGTACATG GGCCCCGGTC AGCTGTACCA AGACCTGCAG 
AACCTAATCC ATGATCTCAG CCTGGTCAAC CAGATCTCCA CCATGATTGG CAGCCTCAAG 
GGAAACTACC AGAATGTAAA TCCCACCGTG GCCCAAGACT GGGTATCAGG ACTCCAGAGG 
CTGATACTGA AGAAGGAGGA GGAGATCAGG GCAGCTGACC GGTGTAGGAT CCAGCTCCAG 
CTGCCTGGCA AACAAGACAA GGCGGGGAAG CCGACCTACT TCAGTGCAGT GTTCAATACC 
CTCAGCCCAG CCATCAAACA GTCATGGATC AGCAACTTGC AGATGGCGAA GCTGGCTCTC 
GAGGAGGACA ATGTGCAGGG CTGGTTCTTT GCAGAGGATG ATGGAAATCA AATGAAGAAG 
CAGAAACACC CCCTGCTGCT TCGACAAATG CCCGTGGTCA TGTCCAAACT GCAGGAGTTT 
AAGGTGGAGT GTGCTGTCCA CAACCCGGAG CCCCACATCA ACACAGACAG CACAGCAGAC 
ACTCCAGTGG TGGGACATGG ATGTGTCTGG GTTGCGAGTT GCACCAACCA GATGGGACAG 
ATTGCCATAG TGGCCATGCA GAACTCCAGC CCTAAGGTGA CCGAGTGTTT CAACGTGGAG 
TCCCGGATTC TCTGCATGGC GTACGTCCCA GCTGAGCAAC GCGAGGAGAA CGGCGAAAAC 
GTCCCTGACG ATAACCAAGT GTCCTCGGCG AAGGCCAGCG ATGCCCCCAG CGTCTGCCTG 
GGCATGGAGG AGGGGAGCAT CATTGTGTAC AAGAGCAGCC AGCGGTCTAA GAAGGTGCGT 
CTACAGCATT TCTTCACCCC AGACAAGTCC ACTGTCACCA GCCTGGTGTA CAGGAAGCAG 
TGCCTGTACG CTGGGCTGGT CAGCGGCTCC GTGGCAATCT ACTCCAGATC AAAAGATGGA 
TCGTGGAACA GCGACAAGCC CAAGTTACTG AAGCTGGGAG TGCTCCCTGT GAAGGCCATG 
CTAGCTGTGG AGGAGCACCT CTGGGCCTCG TCGGGTGGGC AGGTCTTCAT CATCAGCACC 
CACGCACACT CTGTAGAGAG GCAGTTAGAG GCCCACCAAG ATGAGGGCAT GGTGGTGTCA 
CACATGGTGG TGGCCGGGGT GGGCATATGG ATTGCCTTCA GCTCTGGTTC CACGCTGCGC 
CTCTTCCACA CCGAAACCCT AGAGCACCTG CAGGACATCA ACATCGCCAC ACCTGTCCAC 
AGTATACTTC CAGGGAACCA GAGAGTGTCG GTGAGCAGTC TGCTGGTTTG TCATGGTCTG 
CTGCTGGTAG GGACCAACCT GGGAGTGACT GTAGCGCTCT CTGTCCCCAG GCTGCAGGGC 
ATCCCTAAAG TAACAGGTCG GGGCATGGTG TCCTTACATG CTCACAGCGG ACCGGTGAAG 
TTCCTCACCG CTGCCGCTGC AGTGTGTAAC CAGCCGTCAG GCCCACTGCA GCCTGCAGAG 
TCAAAGGATG TGGAGAGCAC CCAGCCTCCA TCAGACTCAG CCCCGACCCC CTCGCAGGGA 
CGAGGGGTGT GGCTGGGAGA GGCGGGCTGC ACTGCCATGG CTCTCCAGGA CTCCCTGTCC 
TCCTCGTCCT GTGGCTCGCT GGCTCCATCC CAGGGTTCGT TGGAGCACGG GCCTGAGGAC 
GGGGCCCTTT ATGATGTGCT CCACGACCCG GCCCATCACC AGAGAGGCCG CCGCTCAAGT 
AAGGTGGATG TCAGCTCTCT CTTGGTGGTC TCTGGAGGTC TGGGACACCG CAGGGTCAAC 
AAGAAGACCA AGCAGTCTCG CCATGAGGAC ACCACCTCTA CCATCATGAT CTGGCAAATC 
CCCTTACTCA ACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_023153848.2 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_023009616.2 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

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CloneID	OMa366281
Clone ID Related Accession (Same CDS sequence)	XM_023153848.2
Accession Version	XM_023153848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036798.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGTC AGTGAAGGCC AGAAAGTATC TGTCAGCGAT 
GCTCGTGCTC CACACGTTAC CCCCGGTTCC TCCTCCCAGG GCAAAGTCAA CCCCTACTCT 
GTGATTGACA TCACTTCCTT CCAGCAGCAG CAGCTTGACC AGCAGCACAG ACCCGCCTCT 
CCCACCCCCT CGCCCACGGA GCCCAAAGAA CGAGAGGCAG ACGGCCAGGA TCCTCCCGGC 
AGCCCTGCTG GCATCGCTTC GGGATACTCG GTGCCTGTCC CGTGTGGCTA TGCGACCCCC 
TCTGGAGTAC CGCTCATCAC CCCAGCTTAC ACCACACCTG TCATCATTCG ACACCTCTCC 
GTGGATGAAG ATGTGATGCT GGTTGGGACT GGGAACGCCT CAGCTGACAG TGTTTTCAGT 
GAAGAGTATC CACCTATCAG AGAAGAGGAT GCTTTGGCAA AATGGGCAGC AGATCCTGCC 
AACACCGCAT GGATGGAAAA TCCACATGAG GTGATTTATG ATGATGTGCC CAGAGAAAAC 
TCCGACTCCA ACACAGATCC AGATGAGATG ATCTACGACG ATGTGGAGCT CGGCGAGGAG 
GGCGGCTGCA ACAGCTCCCT CGACAACGGC TGGAGCTCCA GCGAGTTTGA AAGCTACGAC 
GAAGCCAGCG ACGGGGAAGG TCGGCCTGAG AACGGCCTGC CCCACGCCTT CATGAGAGGG 
AAGCCGCCCC AGAGGAAGAC TCATCTCTCT CAAGATCTGA CACGCTTGAA AGAACACTAT 
GAGAAAAAGA TGAAAGATCT AATGGCAAAC ACAGTGGGAA CGGTAGAGCT GCAGCAGTTA 
AAACAGAAAC ACGAGCAGAA GATGCAAAAG CTGGTGAAGG CTGCCAAGGA AGGAACCAAA 
GATGGACTGG AGAAAACCAA AGCTGCAGTG AAGAAGGGAC GCTCCTTCAT CAAAACCAAG 
TCTTTCTGTC AGGAGAAGTC GGCCTGTTTT GAGGATGAGG AGTCTGGACT CTTCATCGAG 
GTGGACTGTT TCAATGTTGA GCCAGTACTG TGTCCAGCAC CGGAGGCTCT GTCACAGCAG 
CAGCTGGTCA GAAGATGCAT CCTGGGATCT ATTTTGGAGA GCGAGAAGAA TTATCTCGAT 
TCTCTCAAGA GGATTTTAGA GCAATACGAG AAGCCCCTGT CGCAGATCGA GCCCAGACTG 
CTGAGTGACA GGAAACTGAA GATGACTTTC TATCGCGTGC GTGAAATCCT GCAGTGCCAC 
TTCCTGTTCC AGATCGCCCT GGCGAGCCGC GTGGCGGAGT GGGACAGCCT GGAGATGATT 
GGGGATGTCT TTGTGGCGTC GTTCTCTAAG TCCATGGTGC TGGACGCCTA TCGCGAGTAT 
GTGAATAACT TCAGCACTGC GATGGCAGTC GTGAGGAAGA CGTGCGCCTC CAAACCAGCA 
TTCCTGGAAT TCCTCAAGCA TCGTCAGGAA ACCAGCAGTG ACCGAATGAC TCTGTACGGC 
CTGATGATGA AGCCTATCCA GAGATTCCCT CAGTTCATTT TGCTGCTGCA GGACATGTTG 
AAGAACACAC CAGTGGGTCA CCCGGACCGC CTGCCCCTGC AGATGGCCCT GACCGAACTG 
GAGACTCTGG CTGAGAAGCT CAATGAAAAG AAGAGAGAAG CCGACCAGCG CTGCGAGATC 
CGTCACATCG CAAAGGCCAT GAACGAGCGC TACCTCAACA AGCTTCTAAG TAACGGCAGC 
CGCTACTTGA TCCGCTCAGA CGACATGGTG GAAACGGTCT ACAACGAGCG TGGGGAAATC 
ATCAAAACCA AGGAGCGACG CCTGTTCCTG CTCAACGATG TGCTCATGTG TGCCACGCCC 
AACATCCGAT TTCAGGATGG CAGCGGCAGT GGCAGCGGAA GCGTAACACT TGACCAGAAG 
TTCCTTCTGA AGTGGAGCGT GCCTCTGAGC TTCGTGGAGG TGCTTGAGTT CGGGTCCAGC 
GAAGACATGG CCGACACCGG CCGCTTCCCT CCTCCTCATT CGGGCGAGAA AGTGGTGATC 
AGCGCCAAGC CAAATAAACT GTACATGGGC CCCGGTCAGC TGTACCAAGA CCTGCAGAAC 
CTAATCCATG ATCTCAGCCT GGTCAACCAG ATCTCCACCA TGATTGGCAG CCTCAAGGGA 
AACTACCAGA ATGTAAATCC CACCGTGGCC CAAGACTGGG TATCAGGACT CCAGAGGCTG 
ATACTGAAGA AGGAGGAGGA GATCAGGGCA GCTGACCGGT GTAGGATCCA GCTCCAGCTG 
CCTGGCAAAC AAGACAAGGC GGGGAAGCCG ACCTACTTCA GTGCAGTGTT CAATACCCTC 
AGCCCAGCCA TCAAACAGTC ATGGATCAGC AACTTGCAGA TGGCGAAGCT GGCTCTCGAG 
GAGGACAATG TGCAGGGCTG GTTCTTTGCA GAGGATGATG GAAATCAAAT GAAGAAGCAG 
AAACACCCCC TGCTGCTTCG ACAAATGCCC GTGGTCATGT CCAAACTGCA GGAGTTTAAG 
GTGGAGTGTG CTGTCCACAA CCCGGAGCCC CACATCAACA CAGACAGCAC AGCAGACACT 
CCAGTGGTGG GACATGGATG TGTCTGGGTT GCGAGTTGCA CCAACCAGAT GGGACAGATT 
GCCATAGTGG CCATGCAGAA CTCCAGCCCT AAGGTGACCG AGTGTTTCAA CGTGGAGTCC 
CGGATTCTCT GCATGGCGTA CGTCCCAGCT GAGCAACGCG AGGAGAACGG CGAAAACGTC 
CCTGACGATA ACCAAGTGTC CTCGGCGAAG GCCAGCGATG CCCCCAGCGT CTGCCTGGGC 
ATGGAGGAGG GGAGCATCAT TGTGTACAAG AGCAGCCAGC GGTCTAAGAA GGTGCGTCTA 
CAGCATTTCT TCACCCCAGA CAAGTCCACT GTCACCAGCC TGGTGTACAG GAAGCAGTGC 
CTGTACGCTG GGCTGGTCAG CGGCTCCGTG GCAATCTACT CCAGATCAAA AGATGGATCG 
TGGAACAGCG ACAAGCCCAA GTTACTGAAG CTGGGAGTGC TCCCTGTGAA GGCCATGCTA 
GCTGTGGAGG AGCACCTCTG GGCCTCGTCG GGTGGGCAGG TCTTCATCAT CAGCACCCAC 
GCACACTCTG TAGAGAGGCA GTTAGAGGCC CACCAAGATG AGGGCATGGT GGTGTCACAC 
ATGGTGGTGG CCGGGGTGGG CATATGGATT GCCTTCAGCT CTGGTTCCAC GCTGCGCCTC 
TTCCACACCG AAACCCTAGA GCACCTGCAG GACATCAACA TCGCCACACC TGTCCACAGT 
ATACTTCCAG GGAACCAGAG AGTGTCGGTG AGCAGTCTGC TGGTTTGTCA TGGTCTGCTG 
CTGGTAGGGA CCAACCTGGG AGTGACTGTA GCGCTCTCTG TCCCCAGGCT GCAGGGCATC 
CCTAAAGTAA CAGGTCGGGG CATGGTGTCC TTACATGCTC ACAGCGGACC GGTGAAGTTC 
CTCACCGCTG CCGCTGCAGT GTGTAACCAG CCGTCAGGCC CACTGCAGCC TGCAGAGTCA 
AAGGATGTGG AGAGCACCCA GCCTCCATCA GACTCAGCCC CGACCCCCTC GCAGGGACGA 
GGGGTGTGGC TGGGAGAGGC GGGCTGCACT GCCATGGCTC TCCAGGACTC CCTGTCCTCC 
TCGTCCTGTG GCTCGCTGGC TCCATCCCAG GGTTCGTTGG AGCACGGGCC TGAGGACGGG 
GCCCTTTATG ATGTGCTCCA CGACCCGGCC CATCACCAGA GAGGCCGCCG CTCAAGTAAG 
GTGGATGTCA GCTCTCTCTT GGTGGTCTCT GGAGGTCTGG GACACCGCAG GGTCAACAAG 
AAGACCAAGC AGTCTCGCCA TGAGGACACC ACCTCTACCA TCATGATCTG GCAAATCCCC 
TTACTCAACA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009616.2
CDS	263..4537
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETHPAATVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHLSQDLTRLKEHYEKKMKDLMANTVGTVELQQLKQKHEQKMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_023153848.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153848.2

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGTC AGTGAAGGCC AGAAAGTATC TGTCAGCGAT 
GCTCGTGCTC CACACGTTAC CCCCGGTTCC TCCTCCCAGG GCAAAGTCAA CCCCTACTCT 
GTGATTGACA TCACTTCCTT CCAGCAGCAG CAGCTTGACC AGCAGCACAG ACCCGCCTCT 
CCCACCCCCT CGCCCACGGA GCCCAAAGAA CGAGAGGCAG ACGGCCAGGA TCCTCCCGGC 
AGCCCTGCTG GCATCGCTTC GGGATACTCG GTGCCTGTCC CGTGTGGCTA TGCGACCCCC 
TCTGGAGTAC CGCTCATCAC CCCAGCTTAC ACCACACCTG TCATCATTCG ACACCTCTCC 
GTGGATGAAG ATGTGATGCT GGTTGGGACT GGGAACGCCT CAGCTGACAG TGTTTTCAGT 
GAAGAGTATC CACCTATCAG AGAAGAGGAT GCTTTGGCAA AATGGGCAGC AGATCCTGCC 
AACACCGCAT GGATGGAAAA TCCACATGAG GTGATTTATG ATGATGTGCC CAGAGAAAAC 
TCCGACTCCA ACACAGATCC AGATGAGATG ATCTACGACG ATGTGGAGCT CGGCGAGGAG 
GGCGGCTGCA ACAGCTCCCT CGACAACGGC TGGAGCTCCA GCGAGTTTGA AAGCTACGAC 
GAAGCCAGCG ACGGGGAAGG TCGGCCTGAG AACGGCCTGC CCCACGCCTT CATGAGAGGG 
AAGCCGCCCC AGAGGAAGAC TCATCTCTCT CAAGATCTGA CACGCTTGAA AGAACACTAT 
GAGAAAAAGA TGAAAGATCT AATGGCAAAC ACAGTGGGAA CGGTAGAGCT GCAGCAGTTA 
AAACAGAAAC ACGAGCAGAA GATGCAAAAG CTGGTGAAGG CTGCCAAGGA AGGAACCAAA 
GATGGACTGG AGAAAACCAA AGCTGCAGTG AAGAAGGGAC GCTCCTTCAT CAAAACCAAG 
TCTTTCTGTC AGGAGAAGTC GGCCTGTTTT GAGGATGAGG AGTCTGGACT CTTCATCGAG 
GTGGACTGTT TCAATGTTGA GCCAGTACTG TGTCCAGCAC CGGAGGCTCT GTCACAGCAG 
CAGCTGGTCA GAAGATGCAT CCTGGGATCT ATTTTGGAGA GCGAGAAGAA TTATCTCGAT 
TCTCTCAAGA GGATTTTAGA GCAATACGAG AAGCCCCTGT CGCAGATCGA GCCCAGACTG 
CTGAGTGACA GGAAACTGAA GATGACTTTC TATCGCGTGC GTGAAATCCT GCAGTGCCAC 
TTCCTGTTCC AGATCGCCCT GGCGAGCCGC GTGGCGGAGT GGGACAGCCT GGAGATGATT 
GGGGATGTCT TTGTGGCGTC GTTCTCTAAG TCCATGGTGC TGGACGCCTA TCGCGAGTAT 
GTGAATAACT TCAGCACTGC GATGGCAGTC GTGAGGAAGA CGTGCGCCTC CAAACCAGCA 
TTCCTGGAAT TCCTCAAGCA TCGTCAGGAA ACCAGCAGTG ACCGAATGAC TCTGTACGGC 
CTGATGATGA AGCCTATCCA GAGATTCCCT CAGTTCATTT TGCTGCTGCA GGACATGTTG 
AAGAACACAC CAGTGGGTCA CCCGGACCGC CTGCCCCTGC AGATGGCCCT GACCGAACTG 
GAGACTCTGG CTGAGAAGCT CAATGAAAAG AAGAGAGAAG CCGACCAGCG CTGCGAGATC 
CGTCACATCG CAAAGGCCAT GAACGAGCGC TACCTCAACA AGCTTCTAAG TAACGGCAGC 
CGCTACTTGA TCCGCTCAGA CGACATGGTG GAAACGGTCT ACAACGAGCG TGGGGAAATC 
ATCAAAACCA AGGAGCGACG CCTGTTCCTG CTCAACGATG TGCTCATGTG TGCCACGCCC 
AACATCCGAT TTCAGGATGG CAGCGGCAGT GGCAGCGGAA GCGTAACACT TGACCAGAAG 
TTCCTTCTGA AGTGGAGCGT GCCTCTGAGC TTCGTGGAGG TGCTTGAGTT CGGGTCCAGC 
GAAGACATGG CCGACACCGG CCGCTTCCCT CCTCCTCATT CGGGCGAGAA AGTGGTGATC 
AGCGCCAAGC CAAATAAACT GTACATGGGC CCCGGTCAGC TGTACCAAGA CCTGCAGAAC 
CTAATCCATG ATCTCAGCCT GGTCAACCAG ATCTCCACCA TGATTGGCAG CCTCAAGGGA 
AACTACCAGA ATGTAAATCC CACCGTGGCC CAAGACTGGG TATCAGGACT CCAGAGGCTG 
ATACTGAAGA AGGAGGAGGA GATCAGGGCA GCTGACCGGT GTAGGATCCA GCTCCAGCTG 
CCTGGCAAAC AAGACAAGGC GGGGAAGCCG ACCTACTTCA GTGCAGTGTT CAATACCCTC 
AGCCCAGCCA TCAAACAGTC ATGGATCAGC AACTTGCAGA TGGCGAAGCT GGCTCTCGAG 
GAGGACAATG TGCAGGGCTG GTTCTTTGCA GAGGATGATG GAAATCAAAT GAAGAAGCAG 
AAACACCCCC TGCTGCTTCG ACAAATGCCC GTGGTCATGT CCAAACTGCA GGAGTTTAAG 
GTGGAGTGTG CTGTCCACAA CCCGGAGCCC CACATCAACA CAGACAGCAC AGCAGACACT 
CCAGTGGTGG GACATGGATG TGTCTGGGTT GCGAGTTGCA CCAACCAGAT GGGACAGATT 
GCCATAGTGG CCATGCAGAA CTCCAGCCCT AAGGTGACCG AGTGTTTCAA CGTGGAGTCC 
CGGATTCTCT GCATGGCGTA CGTCCCAGCT GAGCAACGCG AGGAGAACGG CGAAAACGTC 
CCTGACGATA ACCAAGTGTC CTCGGCGAAG GCCAGCGATG CCCCCAGCGT CTGCCTGGGC 
ATGGAGGAGG GGAGCATCAT TGTGTACAAG AGCAGCCAGC GGTCTAAGAA GGTGCGTCTA 
CAGCATTTCT TCACCCCAGA CAAGTCCACT GTCACCAGCC TGGTGTACAG GAAGCAGTGC 
CTGTACGCTG GGCTGGTCAG CGGCTCCGTG GCAATCTACT CCAGATCAAA AGATGGATCG 
TGGAACAGCG ACAAGCCCAA GTTACTGAAG CTGGGAGTGC TCCCTGTGAA GGCCATGCTA 
GCTGTGGAGG AGCACCTCTG GGCCTCGTCG GGTGGGCAGG TCTTCATCAT CAGCACCCAC 
GCACACTCTG TAGAGAGGCA GTTAGAGGCC CACCAAGATG AGGGCATGGT GGTGTCACAC 
ATGGTGGTGG CCGGGGTGGG CATATGGATT GCCTTCAGCT CTGGTTCCAC GCTGCGCCTC 
TTCCACACCG AAACCCTAGA GCACCTGCAG GACATCAACA TCGCCACACC TGTCCACAGT 
ATACTTCCAG GGAACCAGAG AGTGTCGGTG AGCAGTCTGC TGGTTTGTCA TGGTCTGCTG 
CTGGTAGGGA CCAACCTGGG AGTGACTGTA GCGCTCTCTG TCCCCAGGCT GCAGGGCATC 
CCTAAAGTAA CAGGTCGGGG CATGGTGTCC TTACATGCTC ACAGCGGACC GGTGAAGTTC 
CTCACCGCTG CCGCTGCAGT GTGTAACCAG CCGTCAGGCC CACTGCAGCC TGCAGAGTCA 
AAGGATGTGG AGAGCACCCA GCCTCCATCA GACTCAGCCC CGACCCCCTC GCAGGGACGA 
GGGGTGTGGC TGGGAGAGGC GGGCTGCACT GCCATGGCTC TCCAGGACTC CCTGTCCTCC 
TCGTCCTGTG GCTCGCTGGC TCCATCCCAG GGTTCGTTGG AGCACGGGCC TGAGGACGGG 
GCCCTTTATG ATGTGCTCCA CGACCCGGCC CATCACCAGA GAGGCCGCCG CTCAAGTAAG 
GTGGATGTCA GCTCTCTCTT GGTGGTCTCT GGAGGTCTGG GACACCGCAG GGTCAACAAG 
AAGACCAAGC AGTCTCGCCA TGAGGACACC ACCTCTACCA TCATGATCTG GCAAATCCCC 
TTACTCAACA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF10 ( XM_014410611.3 ) cDNA ORF clone, Maylandia zebra(Zebra mbuna) -> XP_014266097.2 Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

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CloneID	OMa366282
Clone ID Related Accession (Same CDS sequence)	XM_014410611.3
Accession Version	XM_014410611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036798.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014410611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GATGCTCGTG CTCCACACGT TACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAA 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014266097.2
CDS	262..4422
Translation	MDMEDFPPPPPEMLAGDISFADEDEGEPFDFDDIPEAACPPPALPVSRDQNKDSTVNSQRSDLFPTADPPVPSSAAPSSADGTVATAGGAASEAEGTSAAEGTDDRENDLPPPPLPPLDDDTETHPAATAVSEGQKVSVSDARAPHVTPGSSSQGKVNPYSVIDITSFQQQQLDQQHRPASPTPSPTEPKEREADGQDPPGSPAGIASGYSVPVPCGYATPSGVPLITPAYTTPVIIRHLSVDEDVMLVGTGNASADSVFSEEYPPIREEDALAKWAADPANTAWMENPHEVIYDDVPRENSDSNTDPDEMIYDDVELGEEGGCNSSLDNGWSSSEFESYDEASDGEGRPENGLPHAFMRGKPPQRKTHMQKLVKAAKEGTKDGLEKTKAAVKKGRSFIKTKSFCQEKSACFEDEESGLFIEVDCFNVEPVLCPAPEALSQQQLVRRCILGSILESEKNYLDSLKRILEQYEKPLSQIEPRLLSDRKLKMTFYRVREILQCHFLFQIALASRVAEWDSLEMIGDVFVASFSKSMVLDAYREYVNNFSTAMAVVRKTCASKPAFLEFLKHRQETSSDRMTLYGLMMKPIQRFPQFILLLQDMLKNTPVGHPDRLPLQMALTELETLAEKLNEKKREADQRCEIRHIAKAMNERYLNKLLSNGSRYLIRSDDMVETVYNERGEIIKTKERRLFLLNDVLMCATPNIRFQDGSGSGSGSVTLDQKFLLKWSVPLSFVEVLEFGSSEDMADTGRFPPPHSGEKVVISAKPNKLYMGPGQLYQDLQNLIHDLSLVNQISTMIGSLKGNYQNVNPTVAQDWVSGLQRLILKKEEEIRAADRCRIQLQLPGKQDKAGKPTYFSAVFNTLSPAIKQSWISNLQMAKLALEEDNVQGWFFAEDDGNQMKKQKHPLLLRQMPVVMSKLQEFKVECAVHNPEPHINTDSTADTPVVGHGCVWVASCTNQMGQIAIVAMQNSSPKVTECFNVESRILCMAYVPAEQREENGENVPDDNQVSSAKASDAPSVCLGMEEGSIIVYKSSQRSKKVRLQHFFTPDKSTVTSLVYRKQCLYAGLVSGSVAIYSRSKDGSWNSDKPKLLKLGVLPVKAMLAVEEHLWASSGGQVFIISTHAHSVERQLEAHQDEGMVVSHMVVAGVGIWIAFSSGSTLRLFHTETLEHLQDINIATPVHSILPGNQRVSVSSLLVCHGLLLVGTNLGVTVALSVPRLQGIPKVTGRGMVSLHAHSGPVKFLTAAAAVCNQPSGPLQPAESKDVESTQPPSDSAPTPSQGRGVWLGEAGCTAMALQDSLSSSSCGSLAPSQGSLEHGPEDGALYDVLHDPAHHQRGRRSSKVDVSSLLVVSGGLGHRRVNKKTKQSRHEDTTSTIMIWQIPLLNM

Target ORF information:

RefSeq Version	XM_014410611.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014410611.3

ORF Insert Sequence:

ATGGACATGG AAGACTTTCC TCCCCCACCT CCTGAAATGT TGGCAGGTGA CATCTCTTTT 
GCAGATGAAG ATGAGGGGGA ACCCTTCGAT TTTGACGACA TCCCCGAGGC TGCCTGCCCG 
CCGCCCGCGC TCCCCGTCAG TAGGGATCAG AACAAGGACA GCACTGTGAA CAGCCAACGC 
TCGGACCTCT TCCCCACTGC GGACCCCCCG GTGCCTTCCT CCGCAGCTCC ATCATCAGCA 
GATGGAACAG TTGCCACAGC AGGTGGCGCA GCTTCAGAAG CTGAAGGGAC ATCTGCTGCT 
GAGGGGACTG ATGACAGGGA GAATGATTTG CCTCCGCCTC CGCTCCCTCC TCTGGATGAT 
GACACGGAGA CACATCCTGC AGCAACAGCA GTCAGTGAAG GCCAGAAAGT ATCTGTCAGC 
GATGCTCGTG CTCCACACGT TACCCCCGGT TCCTCCTCCC AGGGCAAAGT CAACCCCTAC 
TCTGTGATTG ACATCACTTC CTTCCAGCAG CAGCAGCTTG ACCAGCAGCA CAGACCCGCC 
TCTCCCACCC CCTCGCCCAC GGAGCCCAAA GAACGAGAGG CAGACGGCCA GGATCCTCCC 
GGCAGCCCTG CTGGCATCGC TTCGGGATAC TCGGTGCCTG TCCCGTGTGG CTATGCGACC 
CCCTCTGGAG TACCGCTCAT CACCCCAGCT TACACCACAC CTGTCATCAT TCGACACCTC 
TCCGTGGATG AAGATGTGAT GCTGGTTGGG ACTGGGAACG CCTCAGCTGA CAGTGTTTTC 
AGTGAAGAGT ATCCACCTAT CAGAGAAGAG GATGCTTTGG CAAAATGGGC AGCAGATCCT 
GCCAACACCG CATGGATGGA AAATCCACAT GAGGTGATTT ATGATGATGT GCCCAGAGAA 
AACTCCGACT CCAACACAGA TCCAGATGAG ATGATCTACG ACGATGTGGA GCTCGGCGAG 
GAGGGCGGCT GCAACAGCTC CCTCGACAAC GGCTGGAGCT CCAGCGAGTT TGAAAGCTAC 
GACGAAGCCA GCGACGGGGA AGGTCGGCCT GAGAACGGCC TGCCCCACGC CTTCATGAGA 
GGGAAGCCGC CCCAGAGGAA GACTCATATG CAAAAGCTGG TGAAGGCTGC CAAGGAAGGA 
ACCAAAGATG GACTGGAGAA AACCAAAGCT GCAGTGAAGA AGGGACGCTC CTTCATCAAA 
ACCAAGTCTT TCTGTCAGGA GAAGTCGGCC TGTTTTGAGG ATGAGGAGTC TGGACTCTTC 
ATCGAGGTGG ACTGTTTCAA TGTTGAGCCA GTACTGTGTC CAGCACCGGA GGCTCTGTCA 
CAGCAGCAGC TGGTCAGAAG ATGCATCCTG GGATCTATTT TGGAGAGCGA GAAGAATTAT 
CTCGATTCTC TCAAGAGGAT TTTAGAGCAA TACGAGAAGC CCCTGTCGCA GATCGAGCCC 
AGACTGCTGA GTGACAGGAA ACTGAAGATG ACTTTCTATC GCGTGCGTGA AATCCTGCAG 
TGCCACTTCC TGTTCCAGAT CGCCCTGGCG AGCCGCGTGG CGGAGTGGGA CAGCCTGGAG 
ATGATTGGGG ATGTCTTTGT GGCGTCGTTC TCTAAGTCCA TGGTGCTGGA CGCCTATCGC 
GAGTATGTGA ATAACTTCAG CACTGCGATG GCAGTCGTGA GGAAGACGTG CGCCTCCAAA 
CCAGCATTCC TGGAATTCCT CAAGCATCGT CAGGAAACCA GCAGTGACCG AATGACTCTG 
TACGGCCTGA TGATGAAGCC TATCCAGAGA TTCCCTCAGT TCATTTTGCT GCTGCAGGAC 
ATGTTGAAGA ACACACCAGT GGGTCACCCG GACCGCCTGC CCCTGCAGAT GGCCCTGACC 
GAACTGGAGA CTCTGGCTGA GAAGCTCAAT GAAAAGAAGA GAGAAGCCGA CCAGCGCTGC 
GAGATCCGTC ACATCGCAAA GGCCATGAAC GAGCGCTACC TCAACAAGCT TCTAAGTAAC 
GGCAGCCGCT ACTTGATCCG CTCAGACGAC ATGGTGGAAA CGGTCTACAA CGAGCGTGGG 
GAAATCATCA AAACCAAGGA GCGACGCCTG TTCCTGCTCA ACGATGTGCT CATGTGTGCC 
ACGCCCAACA TCCGATTTCA GGATGGCAGC GGCAGTGGCA GCGGAAGCGT AACACTTGAC 
CAGAAGTTCC TTCTGAAGTG GAGCGTGCCT CTGAGCTTCG TGGAGGTGCT TGAGTTCGGG 
TCCAGCGAAG ACATGGCCGA CACCGGCCGC TTCCCTCCTC CTCATTCGGG CGAGAAAGTG 
GTGATCAGCG CCAAGCCAAA TAAACTGTAC ATGGGCCCCG GTCAGCTGTA CCAAGACCTG 
CAGAACCTAA TCCATGATCT CAGCCTGGTC AACCAGATCT CCACCATGAT TGGCAGCCTC 
AAGGGAAACT ACCAGAATGT AAATCCCACC GTGGCCCAAG ACTGGGTATC AGGACTCCAG 
AGGCTGATAC TGAAGAAGGA GGAGGAGATC AGGGCAGCTG ACCGGTGTAG GATCCAGCTC 
CAGCTGCCTG GCAAACAAGA CAAGGCGGGG AAGCCGACCT ACTTCAGTGC AGTGTTCAAT 
ACCCTCAGCC CAGCCATCAA ACAGTCATGG ATCAGCAACT TGCAGATGGC GAAGCTGGCT 
CTCGAGGAGG ACAATGTGCA GGGCTGGTTC TTTGCAGAGG ATGATGGAAA TCAAATGAAG 
AAGCAGAAAC ACCCCCTGCT GCTTCGACAA ATGCCCGTGG TCATGTCCAA ACTGCAGGAG 
TTTAAGGTGG AGTGTGCTGT CCACAACCCG GAGCCCCACA TCAACACAGA CAGCACAGCA 
GACACTCCAG TGGTGGGACA TGGATGTGTC TGGGTTGCGA GTTGCACCAA CCAGATGGGA 
CAGATTGCCA TAGTGGCCAT GCAGAACTCC AGCCCTAAGG TGACCGAGTG TTTCAACGTG 
GAGTCCCGGA TTCTCTGCAT GGCGTACGTC CCAGCTGAGC AACGCGAGGA GAACGGCGAA 
AACGTCCCTG ACGATAACCA AGTGTCCTCG GCGAAGGCCA GCGATGCCCC CAGCGTCTGC 
CTGGGCATGG AGGAGGGGAG CATCATTGTG TACAAGAGCA GCCAGCGGTC TAAGAAGGTG 
CGTCTACAGC ATTTCTTCAC CCCAGACAAG TCCACTGTCA CCAGCCTGGT GTACAGGAAG 
CAGTGCCTGT ACGCTGGGCT GGTCAGCGGC TCCGTGGCAA TCTACTCCAG ATCAAAAGAT 
GGATCGTGGA ACAGCGACAA GCCCAAGTTA CTGAAGCTGG GAGTGCTCCC TGTGAAGGCC 
ATGCTAGCTG TGGAGGAGCA CCTCTGGGCC TCGTCGGGTG GGCAGGTCTT CATCATCAGC 
ACCCACGCAC ACTCTGTAGA GAGGCAGTTA GAGGCCCACC AAGATGAGGG CATGGTGGTG 
TCACACATGG TGGTGGCCGG GGTGGGCATA TGGATTGCCT TCAGCTCTGG TTCCACGCTG 
CGCCTCTTCC ACACCGAAAC CCTAGAGCAC CTGCAGGACA TCAACATCGC CACACCTGTC 
CACAGTATAC TTCCAGGGAA CCAGAGAGTG TCGGTGAGCA GTCTGCTGGT TTGTCATGGT 
CTGCTGCTGG TAGGGACCAA CCTGGGAGTG ACTGTAGCGC TCTCTGTCCC CAGGCTGCAG 
GGCATCCCTA AAGTAACAGG TCGGGGCATG GTGTCCTTAC ATGCTCACAG CGGACCGGTG 
AAGTTCCTCA CCGCTGCCGC TGCAGTGTGT AACCAGCCGT CAGGCCCACT GCAGCCTGCA 
GAGTCAAAGG ATGTGGAGAG CACCCAGCCT CCATCAGACT CAGCCCCGAC CCCCTCGCAG 
GGACGAGGGG TGTGGCTGGG AGAGGCGGGC TGCACTGCCA TGGCTCTCCA GGACTCCCTG 
TCCTCCTCGT CCTGTGGCTC GCTGGCTCCA TCCCAGGGTT CGTTGGAGCA CGGGCCTGAG 
GACGGGGCCC TTTATGATGT GCTCCACGAC CCGGCCCATC ACCAGAGAGG CCGCCGCTCA 
AGTAAGGTGG ATGTCAGCTC TCTCTTGGTG GTCTCTGGAG GTCTGGGACA CCGCAGGGTC 
AACAAGAAGA CCAAGCAGTC TCGCCATGAG GACACCACCT CTACCATCAT GATCTGGCAA 
ATCCCCTTAC TCAACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

ARHGEF10 cDNA ORF clone, Maylandia zebra(Zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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