LOC101479339 gene cDNA ORF clone, Maylandia zebra(zebra mbuna)

mRNA	Protein	Name
XM_023152522.2	XP_023008290.2	supervillin isoform X2
XM_023152523.1	XP_023008291.1	supervillin isoform X6
XM_004552382.2	XP_004552439.1	supervillin-like isoform X1
XM_004552386.3	XP_004552443.2	supervillin isoform X5
XM_004552386.2	XP_004552443.1	supervillin isoform X5
XM_023152523.2	XP_023008291.2	supervillin isoform X6
XM_004552381.3	XP_004552438.2	supervillin isoform X1
XM_004552382.3	XP_004552439.1	supervillin isoform X1
XM_004552385.2	XP_004552442.1	supervillin isoform X4
XM_023152522.1	XP_023008290.1	supervillin isoform X2
XM_004552382.4	XP_004552439.2	supervillin isoform X1
XM_004552383.2	XP_004552440.1	supervillin-like isoform X2
XM_004552384.3	XP_004552441.2	supervillin isoform X3
XM_004552381.2	XP_004552438.1	supervillin isoform X1
XM_004552384.2	XP_004552441.1	supervillin isoform X3
XM_004552385.3	XP_004552442.2	supervillin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa312371	XM_023152522.1 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa312372	XM_023152523.1 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34409	XM_004552382.3 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34409	XM_004552381.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34410	XM_004552383.2 Latest version!	Maylandia zebra supervillin-like (LOC101479339), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34411	XM_004552384.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34412	XM_004552385.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34413	XM_004552386.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372239	XM_004552382.4 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372239	XM_004552381.3 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372240	XM_023152522.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372241	XM_004552384.3 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372242	XM_004552385.3 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372243	XM_004552386.3 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa372244	XM_023152523.2 Latest version!	Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMa34409	XM_004552382.2	Maylandia zebra supervillin-like (LOC101479339), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

LOC101479339 ( XM_023152522.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008290.1 Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMa312371
Clone ID Related Accession (Same CDS sequence)	XM_023152522.1
Accession Version	XM_023152522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008290.1
CDS	307..5268
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKDVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_023152522.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152522.1

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_023152523.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008291.1 Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.

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CloneID	OMa312372
Clone ID Related Accession (Same CDS sequence)	XM_023152523.1
Accession Version	XM_023152523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008291.1
CDS	307..5088
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKACLDPENEENSKDVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_023152523.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152523.1

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552382.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552439.1 Maylandia zebra supervillin-like (LOC101479339), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa34409
Clone ID Related Accession (Same CDS sequence)	XM_004552382.2 , XM_004552382.3 , XM_004552381.2
Accession Version	XM_004552382.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	supervillin-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552439.1
CDS	375..5339
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552382.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra supervillin-like (LOC101479339), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552382.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552382.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552439.1 Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.

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CloneID	OMa34409
Clone ID Related Accession (Same CDS sequence)	XM_004552382.2 , XM_004552382.3 , XM_004552381.2
Accession Version	XM_004552382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004552382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552439.1
CDS	380..5344
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552382.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552382.3

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552381.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552438.1 Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.

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CloneID	OMa34409
Clone ID Related Accession (Same CDS sequence)	XM_004552382.2 , XM_004552382.3 , XM_004552381.2
Accession Version	XM_004552381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552438.1
CDS	307..5271
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552381.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552381.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552383.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552440.1 Maylandia zebra supervillin-like (LOC101479339), transcript variant X3, mRNA.

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CloneID	OMa34410
Clone ID Related Accession (Same CDS sequence)	XM_004552383.2
Accession Version	XM_004552383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	supervillin-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCCTTCC CTGACGCTGG GCAAGCCACA TCCTCTCTGG ATCTGGCTGT AAACCCGGGC 
TCTGAGGGGG GTCGGCGACG CACTCGGCGT TACCTCCCTA GCTCGTCAGG CGGTAACAGA 
AAAACTAGCG AACGCTTCAG GACACAGCCA ATCACAGCCA ATGAGATGGA GGAGAGCAGT 
GGGTGCTTGG ATCCAGAGAA CGAGGAAAAC AGCAAAGCAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552440.1
CDS	307..5268
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKAFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552383.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra supervillin-like (LOC101479339), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552383.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCCTTCC CTGACGCTGG GCAAGCCACA TCCTCTCTGG ATCTGGCTGT AAACCCGGGC 
TCTGAGGGGG GTCGGCGACG CACTCGGCGT TACCTCCCTA GCTCGTCAGG CGGTAACAGA 
AAAACTAGCG AACGCTTCAG GACACAGCCA ATCACAGCCA ATGAGATGGA GGAGAGCAGT 
GGGTGCTTGG ATCCAGAGAA CGAGGAAAAC AGCAAAGCAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC CTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGACAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552384.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552441.1 Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.

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CloneID	OMa34411
Clone ID Related Accession (Same CDS sequence)	XM_004552384.2
Accession Version	XM_004552384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGCT CCAGAAAGGC 
CCTGTTCAGC TTCCTGCCCT CTGTTTGTCC CCTCATCTGT CCGACAGACA GCAGGCCTCG 
TCTGTCAACC TGAAACCCAG TGAGGTACGT CTTTCACAGT CCACATCAGA TGCTGGCACT 
GGACTCGGAA AATCTGGTCC GACTCAACAG GGACCACAGT GCTACGACTC TGAGCCAGGC 
TTAAAAGGAA TCCTGAAGAA GAGTCGCTCT GCAGGCTCGG AGTGGAGCAG GACAGAAGCC 
AGTCAGGTAG ATGCATCATA CAGCCATGAA CAGAATGGTG GAGGCTGTAG AGAAGCGGCG 
ATACACGGGA GCAAAGCGGG AGGTGAGAGG GAGGGAACTT CTGCAGCACC ACAGAGAGAC 
AGACGAGAGA CCTCTGGTAT GGAGGAAGGT TCGCAGTCTG CTGCTCCCTG GAGGCAGAGG 
GCTAGAAACA GGAGGGAAAC CATTGCCTGC ACACCAATAA GAGCCTTGTC AGAGCAGGAT 
GCTCCACAGC AATCCTGCCA GTCCAAATAT GAACTGAGCT TTTCCTCTGA GAAAAACAGC 
GTAGACATCA ATGGAAGAGC CAAGCAGGAG GAAGACCAAA AGGCCATGAA CAAGATCCAT 
GACAACACCA CAGATATTAA AGCTGTCCGG GTCACCTCAG AAGAGTGTGC GCCTAAAATG 
CAGGAGACAA CCAACGGCAG CAGAAGTGAG TATCATGTGC AGGAACAGTC AAAAAACCCT 
CATCTTGAGA ATGACAACCT TCAGTGCTGG GAGCCCGTCT TTGCCTCTGT CTATTCTATC 
AATACGCCCC AATATATCAT GTGTTTTAAT CAGACTAACC TCTCCTTTGA GGCACAAGAA 
GTCTCCTCTC CTACGAACAA TCAGAGTCAG CCTCAGTGGA GACATAAGGA TAAAGGAACT 
GAGATTCAGA TGACTGAATT TGGAGAATTG AAAACACAGC AGGAAAGCAA AGAGCAGGAG 
GCTGGAAGCA CGGCTAAGAC AAGCCTTGCA TCTGGTGAAT CACAGAGAAA TGGTATCGCT 
CACAGCAGGA GACACTCCAC TGATGCTCCA TCATGCAAAA ATGAAGTGAC TCCACAGAGT 
TCTTCACCAA CAGTCACTAC TCAGGAGGTA AAGGAAGAGA CGCCTGTTGA TGAACCAAAC 
ATGTTTGATG GCAGTTTCTA CTGTGATCAG TCACCTTCTT CAGCCTGCCC CCCACCTTGT 
GGTGGAATTG CTGCCTCAGA GGGTGAGCAG GACCTGGGAG TCTTCTGCCA GACCAACACA 
CACATATTGA CCTCAGCAGT AGCAGAGCAC AGGCGGTCGG TACGTCCGTC CCGTCGGACT 
CAAGGCTCCA GAAACCCTCT GAGGGCTCTC GCAGCCAGAG AGGACATCAG GCAGGACTTC 
ATGGGAGAGA GAGTCAATTC ATCAGCTCAA GAGAGGATCC AAGCAGAAAA AAAGTCTAAG 
AACTCGTCTA TAGCAGACTC ACCTTCCTTA AGATCTGAAG AATCCTCCTT AGATGCCGAG 
ACAGCTAAGT CTTACCCGCC CTTCAGCAGC CTGATGCTAA TTCACATCAA AGGTAGGCGG 
CATGTTCAGG TCCGCCTGGT TGAGCCCTCA GTGCGGTCAC TGAACAGTGG AGACTGCTTC 
CTGCTGGTCA CTCCAGAGCA CTGCATCCTG TGGAGTGGAG AGTTTGCCAA CGGACAAGAA 
AAAGCAAAGG CCTCTGAGCT AGCTTTGTTC ATCCAGAACC AAAGGGACCT TGGTTGTTTG 
GCTTCCCACG TTGTCCACCT GCAGGAGGGC ATGAACTCTG ACAGCAGTGT GGCTGCAGAC 
TTCTGGAGTC TCCTGGGAGG AAGAACACAG TACAGAGGAG CTTGTGCTCC AGAGGAGGAT 
GAGCTCTATG AGCGCGGTGT GGTGGAGTCC AACTGTGTGT ACAGGCTGGT GGAAAACAAA 
CTGGTGCCTC ATGAACAGGC TTGGGCCTCC ATCCCCAGCA TCACCCTGTT GGCCTCCTCT 
GAGGCCCTGG TGTTTGATTT CGGCAGTGAG GTCTATCTGT GGCTCGGACA GGACGTTTCC 
CTCAGGAGGA GAAATGTTGC TCTCCAGTTG ACTCACCAGG TGTGGGTTGG AGCTTATGAC 
TACAGCAACT GTCAAGTAAA CCCACTGGAT CCCACTCAGT GTAACCCTGC AATTCAGCTA 
CGAGGAGAAG GCCGTCCCAG CTGGGCTCTG TTCGGCGTCC TCACTGAGAG CAACGAGACA 
GCTCTGTTCA GGGAGAAGTT CCTGGACTGG ATGTGCAGGG TTGGAGGCAG AGAGGAAGCG 
GCTTCAGGGA CGGATCAGAC GCAGTCCATC CCAGTCCAGT CATCCCAGCC GCTCTCCCCT 
CCTTCAGACC TCTTGAGTCC CTGCGATGCC AAAGCTCTAG TATCGGGTCA GTGCTTAGAA 
GGTGACGGTT TGGTCCGTAC TGTGCTGGCT GGGGTCGATG TCCAGAGAGG TCATGGTGTC 
ATCATGCTGA CGGAGGAACG TCAGATGGAG CTTAACACAG TAGCGGTGGA CACCTGGCAC 
GTCCAGGAGT TTGACGACAG TGAAATCCCA GTGGAGAGCA ACGGACAGCT TTATGAAGGA 
GACTCTTATG TAATCCGCTG GAAGTACAGC ATCAGTACAG CTGACAGCAC AGACAGCTCA 
GATGAGTGTG GGACGGATCC AGCACCAAAG GAAAAGACGG CCTTCTTCCT GTGGCGAGGC 
CGTCACTCCA GTGTCAGTGG ACGGGACACA GCTGCTTTCC TTTCCATCGG GATGAGGAAC 
CACGAAGAGT CGCAGGTGGT TGTGCCTCAG GGAAAAGAGC CCCCCTGTTT CCTGCAGCTT 
TTCAAGGGAG GCTTGGTCAT TCACAAAGGA AAGCAAGAGG AAGCTTCCAT CAACACAGAA 
AGCTGGCGTC TGTTTTGTGT GCGAGGGGAG CTCCCAGAAG AGGGTTTTCT GTTAGAGGTG 
GACTGCTGCT GTGCGGGTCT GAGGTCGAGA GGCTGTGTTG TCCTGCTAAA TGGCCAGCAG 
GGAGTGCTCT ATCTCTGGAT TGGTTGTAAG GCTCACAGCA GCACAAAGGA AGTCGGCAAG 
AGGGTGGTGG AGGTCCTCAC TAAAATCTGT CCATCAGAGG TGGGTTTAAG CAAAAGCAGC 
CCTGTAAAGG TGCAGATAGT ACAGGAAGGT TCAGAGCCAG AAGACTTCTG GACAGCACTG 
GGACAAAAGG ACAGGAAGGC CTATGACTGC ATGCTGCAAG ATCCAGGAAA GTATAACTTC 
ACGCCTCGCC TCTTCCACCT GAGTGCCTCT TCTGGGAGTT TCCAGGCCGA GGAGCTGCAG 
AGTCCGATGC AGATGCCGGG GCTTGTGATG GCAATGCCTT TTGTCCAGGA GAGCCTATAC 
AGTGTACCAC AACCAGCTTT GTTCCTTCTT GACAACCGTC TGGAGGTCTA CTTGTGGCAG 
AGAGGTCAGC CTGAGCAGAC TGACAGCTCC TCAGCCTGGA AACTCTGGCA TCATGAGAGG 
AAGTGTGCCA TGCAGACAGC GCTGCAGTAC TGCAAAGAAA TAAACCAGAG AAGGCCGCCA 
CATGCGTATC TCATCCTCGA GGGGGCTGAG CCTCTCACCT TCACTAATGT CTTCCCGCGC 
TGGGAGAAAA GCCTTGAACC TCATGCACAG GGTGATACAG GGCGAGCGAA GCTGACCTTG 
GTGCAGGACA CCCTGGCCCA GCTCATGAAG ACCCAGTATC CTCTGGAGGA GCTGCTGCGG 
AGCCCCCTTC CTGAAGGAGT GGACCCCCAG CGCCTGGAGG TCTACCTGTC CAATGAGGAC 
TTTCAGACTA TTTTGGAAAT GAAGAGAGAT GAATATGCCT CCCTCCCAAG CTGGAAGCAA 
ATTGATCTGA AGAAGAGCAA AGGGCTGTTT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552441.1
CDS	307..5262
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEELQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552384.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552384.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGCT CCAGAAAGGC 
CCTGTTCAGC TTCCTGCCCT CTGTTTGTCC CCTCATCTGT CCGACAGACA GCAGGCCTCG 
TCTGTCAACC TGAAACCCAG TGAGGTACGT CTTTCACAGT CCACATCAGA TGCTGGCACT 
GGACTCGGAA AATCTGGTCC GACTCAACAG GGACCACAGT GCTACGACTC TGAGCCAGGC 
TTAAAAGGAA TCCTGAAGAA GAGTCGCTCT GCAGGCTCGG AGTGGAGCAG GACAGAAGCC 
AGTCAGGTAG ATGCATCATA CAGCCATGAA CAGAATGGTG GAGGCTGTAG AGAAGCGGCG 
ATACACGGGA GCAAAGCGGG AGGTGAGAGG GAGGGAACTT CTGCAGCACC ACAGAGAGAC 
AGACGAGAGA CCTCTGGTAT GGAGGAAGGT TCGCAGTCTG CTGCTCCCTG GAGGCAGAGG 
GCTAGAAACA GGAGGGAAAC CATTGCCTGC ACACCAATAA GAGCCTTGTC AGAGCAGGAT 
GCTCCACAGC AATCCTGCCA GTCCAAATAT GAACTGAGCT TTTCCTCTGA GAAAAACAGC 
GTAGACATCA ATGGAAGAGC CAAGCAGGAG GAAGACCAAA AGGCCATGAA CAAGATCCAT 
GACAACACCA CAGATATTAA AGCTGTCCGG GTCACCTCAG AAGAGTGTGC GCCTAAAATG 
CAGGAGACAA CCAACGGCAG CAGAAGTGAG TATCATGTGC AGGAACAGTC AAAAAACCCT 
CATCTTGAGA ATGACAACCT TCAGTGCTGG GAGCCCGTCT TTGCCTCTGT CTATTCTATC 
AATACGCCCC AATATATCAT GTGTTTTAAT CAGACTAACC TCTCCTTTGA GGCACAAGAA 
GTCTCCTCTC CTACGAACAA TCAGAGTCAG CCTCAGTGGA GACATAAGGA TAAAGGAACT 
GAGATTCAGA TGACTGAATT TGGAGAATTG AAAACACAGC AGGAAAGCAA AGAGCAGGAG 
GCTGGAAGCA CGGCTAAGAC AAGCCTTGCA TCTGGTGAAT CACAGAGAAA TGGTATCGCT 
CACAGCAGGA GACACTCCAC TGATGCTCCA TCATGCAAAA ATGAAGTGAC TCCACAGAGT 
TCTTCACCAA CAGTCACTAC TCAGGAGGTA AAGGAAGAGA CGCCTGTTGA TGAACCAAAC 
ATGTTTGATG GCAGTTTCTA CTGTGATCAG TCACCTTCTT CAGCCTGCCC CCCACCTTGT 
GGTGGAATTG CTGCCTCAGA GGGTGAGCAG GACCTGGGAG TCTTCTGCCA GACCAACACA 
CACATATTGA CCTCAGCAGT AGCAGAGCAC AGGCGGTCGG TACGTCCGTC CCGTCGGACT 
CAAGGCTCCA GAAACCCTCT GAGGGCTCTC GCAGCCAGAG AGGACATCAG GCAGGACTTC 
ATGGGAGAGA GAGTCAATTC ATCAGCTCAA GAGAGGATCC AAGCAGAAAA AAAGTCTAAG 
AACTCGTCTA TAGCAGACTC ACCTTCCTTA AGATCTGAAG AATCCTCCTT AGATGCCGAG 
ACAGCTAAGT CTTACCCGCC CTTCAGCAGC CTGATGCTAA TTCACATCAA AGGTAGGCGG 
CATGTTCAGG TCCGCCTGGT TGAGCCCTCA GTGCGGTCAC TGAACAGTGG AGACTGCTTC 
CTGCTGGTCA CTCCAGAGCA CTGCATCCTG TGGAGTGGAG AGTTTGCCAA CGGACAAGAA 
AAAGCAAAGG CCTCTGAGCT AGCTTTGTTC ATCCAGAACC AAAGGGACCT TGGTTGTTTG 
GCTTCCCACG TTGTCCACCT GCAGGAGGGC ATGAACTCTG ACAGCAGTGT GGCTGCAGAC 
TTCTGGAGTC TCCTGGGAGG AAGAACACAG TACAGAGGAG CTTGTGCTCC AGAGGAGGAT 
GAGCTCTATG AGCGCGGTGT GGTGGAGTCC AACTGTGTGT ACAGGCTGGT GGAAAACAAA 
CTGGTGCCTC ATGAACAGGC TTGGGCCTCC ATCCCCAGCA TCACCCTGTT GGCCTCCTCT 
GAGGCCCTGG TGTTTGATTT CGGCAGTGAG GTCTATCTGT GGCTCGGACA GGACGTTTCC 
CTCAGGAGGA GAAATGTTGC TCTCCAGTTG ACTCACCAGG TGTGGGTTGG AGCTTATGAC 
TACAGCAACT GTCAAGTAAA CCCACTGGAT CCCACTCAGT GTAACCCTGC AATTCAGCTA 
CGAGGAGAAG GCCGTCCCAG CTGGGCTCTG TTCGGCGTCC TCACTGAGAG CAACGAGACA 
GCTCTGTTCA GGGAGAAGTT CCTGGACTGG ATGTGCAGGG TTGGAGGCAG AGAGGAAGCG 
GCTTCAGGGA CGGATCAGAC GCAGTCCATC CCAGTCCAGT CATCCCAGCC GCTCTCCCCT 
CCTTCAGACC TCTTGAGTCC CTGCGATGCC AAAGCTCTAG TATCGGGTCA GTGCTTAGAA 
GGTGACGGTT TGGTCCGTAC TGTGCTGGCT GGGGTCGATG TCCAGAGAGG TCATGGTGTC 
ATCATGCTGA CGGAGGAACG TCAGATGGAG CTTAACACAG TAGCGGTGGA CACCTGGCAC 
GTCCAGGAGT TTGACGACAG TGAAATCCCA GTGGAGAGCA ACGGACAGCT TTATGAAGGA 
GACTCTTATG TAATCCGCTG GAAGTACAGC ATCAGTACAG CTGACAGCAC AGACAGCTCA 
GATGAGTGTG GGACGGATCC AGCACCAAAG GAAAAGACGG CCTTCTTCCT GTGGCGAGGC 
CGTCACTCCA GTGTCAGTGG ACGGGACACA GCTGCTTTCC TTTCCATCGG GATGAGGAAC 
CACGAAGAGT CGCAGGTGGT TGTGCCTCAG GGAAAAGAGC CCCCCTGTTT CCTGCAGCTT 
TTCAAGGGAG GCTTGGTCAT TCACAAAGGA AAGCAAGAGG AAGCTTCCAT CAACACAGAA 
AGCTGGCGTC TGTTTTGTGT GCGAGGGGAG CTCCCAGAAG AGGGTTTTCT GTTAGAGGTG 
GACTGCTGCT GTGCGGGTCT GAGGTCGAGA GGCTGTGTTG TCCTGCTAAA TGGCCAGCAG 
GGAGTGCTCT ATCTCTGGAT TGGTTGTAAG GCTCACAGCA GCACAAAGGA AGTCGGCAAG 
AGGGTGGTGG AGGTCCTCAC TAAAATCTGT CCATCAGAGG TGGGTTTAAG CAAAAGCAGC 
CCTGTAAAGG TGCAGATAGT ACAGGAAGGT TCAGAGCCAG AAGACTTCTG GACAGCACTG 
GGACAAAAGG ACAGGAAGGC CTATGACTGC ATGCTGCAAG ATCCAGGAAA GTATAACTTC 
ACGCCTCGCC TCTTCCACCT GAGTGCCTCT TCTGGGAGTT TCCAGGCCGA GGAGCTGCAG 
AGTCCGATGC AGATGCCGGG GCTTGTGATG GCAATGCCTT TTGTCCAGGA GAGCCTATAC 
AGTGTACCAC AACCAGCTTT GTTCCTTCTT GACAACCGTC TGGAGGTCTA CTTGTGGCAG 
AGAGGTCAGC CTGAGCAGAC TGACAGCTCC TCAGCCTGGA AACTCTGGCA TCATGAGAGG 
AAGTGTGCCA TGCAGACAGC GCTGCAGTAC TGCAAAGAAA TAAACCAGAG AAGGCCGCCA 
CATGCGTATC TCATCCTCGA GGGGGCTGAG CCTCTCACCT TCACTAATGT CTTCCCGCGC 
TGGGAGAAAA GCCTTGAACC TCATGCACAG GGTGATACAG GGCGAGCGAA GCTGACCTTG 
GTGCAGGACA CCCTGGCCCA GCTCATGAAG ACCCAGTATC CTCTGGAGGA GCTGCTGCGG 
AGCCCCCTTC CTGAAGGAGT GGACCCCCAG CGCCTGGAGG TCTACCTGTC CAATGAGGAC 
TTTCAGACTA TTTTGGAAAT GAAGAGAGAT GAATATGCCT CCCTCCCAAG CTGGAAGCAA 
ATTGATCTGA AGAAGAGCAA AGGGCTGTTT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552385.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552442.1 Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMa34412
Clone ID Related Accession (Same CDS sequence)	XM_004552385.2
Accession Version	XM_004552385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552442.1
CDS	307..5208
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552385.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552385.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGACGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGAATCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552386.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552443.1 Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.

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CloneID	OMa34413
Clone ID Related Accession (Same CDS sequence)	XM_004552386.2
Accession Version	XM_004552386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552443.1
CDS	307..5091
Translation	MMENAILEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKACLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQETTNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGESQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552386.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552386.2

ORF Insert Sequence:

ATGATGGAGA ACGCCATCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCCTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGACAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGAATC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552381.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552438.2 Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.

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CloneID	OMa372239
Clone ID Related Accession (Same CDS sequence)	XM_004552381.3 , XM_004552382.4
Accession Version	XM_004552381.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552438.2
CDS	307..5271
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552381.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552381.3

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552382.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552439.2 Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.

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CloneID	OMa372239
Clone ID Related Accession (Same CDS sequence)	XM_004552381.3 , XM_004552382.4
Accession Version	XM_004552382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552439.2
CDS	380..5344
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552382.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552382.4

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_023152522.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008290.2 Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.

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CloneID	OMa372240
Clone ID Related Accession (Same CDS sequence)	XM_023152522.2
Accession Version	XM_023152522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023152522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC TTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGGCAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008290.2
CDS	307..5268
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKDVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_023152522.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152522.2

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC TTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGGCAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552384.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552441.2 Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.

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CloneID	OMa372241
Clone ID Related Accession (Same CDS sequence)	XM_004552384.3
Accession Version	XM_004552384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGCT CCAGAAAGGC 
CCTGTTCAGC TTCCTGCCCT CTGTTTGTCC CCTCATCTGT CCGACAGACA GCAGGCCTCG 
TCTGTCAACC TGAAACCCAG TGAGGTACGT CTTTCACAGT CCACATCAGA TGCTGGCACT 
GGACTCGGAA AATCTGGTCC GACTCAACAG GGACCACAGT GCTACGACTC TGAGCCAGGC 
TTAAAAGGAA TCCTGAAGAA GAGTCGCTCT GCAGGCTCGG AGTGGAGCAG GACAGAAGCC 
AGTCAGGTAG ATGCATCATA CAGCCATGAA CAGAATGGTG GAGGCTGTAG AGAAGCGGCG 
ATACACGGGA GCAAAGCGGG AGGTGAGAGG GAGGGAACTT CTGCAGCACC ACAGAGAGAC 
AGACGAGAGA CCTCTGGTAT GGAGGAAGGT TCGCAGTCTG CTGCTCCCTG GAGGCAGAGG 
GCTAGAAACA GGAGGGAAAC CATTGCCTGC ACACCAATAA GAGCCTTGTC AGAGCAGGAT 
GCTCCACAGC AATCCTGCCA GTCCAAATAT GAACTGAGCT TTTCTTCTGA GAAAAACAGC 
GTAGACATCA ATGGAAGAGC CAAGCAGGAG GAAGACCAAA AGGCCATGAA CAAGATCCAT 
GACAACACCA CAGATATTAA AGCTGTCCGG GTCACCTCAG AAGAGTGTGC GCCTAAAATG 
CAGGAGGCAA CCAACGGCAG CAGAAGTGAG TATCATGTGC AGGAACAGTC AAAAAACCCT 
CATCTTGAGA ATGACAACCT TCAGTGCTGG GAGCCCGTCT TTGCCTCTGT CTATTCTATC 
AATACGCCCC AATATATCAT GTGTTTTAAT CAGACTAACC TCTCCTTTGA GGCACAAGAA 
GTCTCCTCTC CTACGAACAA TCAGAGTCAG CCTCAGTGGA GACATAAGGA TAAAGGAACT 
GAGATTCAGA TGACTGAATT TGGAGAATTG AAAACACAGC AGGAAAGCAA AGAGCAGGAG 
GCTGGAAGCA CGGCTAAGAC AAGCCTTGCA TCTGGTGACT CACAGAGAAA TGGTATCGCT 
CACAGCAGGA GACACTCCAC TGATGCTCCA TCATGCAAAA ATGAAGTGAC TCCACAGAGT 
TCTTCACCAA CAGTCACTAC TCAGGAGGTA AAGGAAGAGA CGCCTGTTGA TGAACCAAAC 
ATGTTTGATG GCAGTTTCTA CTGTGATCAG TCACCTTCTT CAGCCTGCCC CCCACCTTGT 
GGTGGAATTG CTGCCTCAGA GGGTGAGCAG GACCTGGGAG TCTTCTGCCA GACCAACACA 
CACATATTGA CCTCAGCAGT AGCAGAGCAC AGGCGGTCGG TACGTCCGTC CCGTCGGACT 
CAAGGCTCCA GAAACCCTCT GAGGGCTCTC GCAGCCAGAG AGGACATCAG GCAGGACTTC 
ATGGGAGAGA GAGTCAATTC ATCAGCTCAA GAGAGGATCC AAGCAGAAAA AAAGTCTAAG 
AACTCGTCTA TAGCAGACTC ACCTTCCTTA AGATCTGAAG AATCCTCCTT AGATGCCGAG 
ACAGCTAAGT CTTACCCGCC CTTCAGCAGC CTGATGCTAA TTCACATCAA AGGTAGGCGG 
CATGTTCAGG TCCGCCTGGT TGAGCCCTCA GTGCGGTCAC TGAACAGTGG AGACTGCTTC 
CTGCTGGTCA CTCCAGAGCA CTGCATCCTG TGGAGTGGAG AGTTTGCCAA CGGACAAGAA 
AAAGCAAAGG CCTCTGAGCT AGCTTTGTTC ATCCAGAACC AAAGGGACCT TGGTTGTTTG 
GCTTCCCACG TTGTCCACCT GCAGGAGGGC ATGAACTCTG ACAGCAGTGT GGCTGCAGAC 
TTCTGGAGTC TCCTGGGAGG AAGAACACAG TACAGAGGAG CTTGTGCTCC AGAGGAGGAT 
GAGCTCTATG AGCGCGGTGT GGTGGAGTCC AACTGTGTGT ACAGGCTGGT GGAAAACAAA 
CTGGTGCCTC ATGAACAGGC TTGGGCCTCC ATCCCCAGCA TCACCCTGTT GGCCTCCTCT 
GAGGCCCTGG TGTTTGATTT CGGCAGTGAG GTCTATCTGT GGCTCGGACA GGACGTTTCC 
CTCAGGAGGA GAAATGTTGC TCTCCAGTTG ACTCACCAGG TGTGGGTTGG AGCTTATGAC 
TACAGCAACT GTCAAGTAAA CCCACTGGAT CCCACTCAGT GTAACCCTGC AATTCAGCTA 
CGAGGAGAAG GCCGTCCCAG CTGGGCTCTG TTCGGCGTCC TCACTGAGAG CAACGAGACA 
GCTCTGTTCA GGGAGAAGTT CCTGGACTGG ATGTGCAGGG TTGGAGGCAG AGAGGAAGCG 
GCTTCAGGGA CGGATCAGAC GCAGTCCATC CCAGTCCAGT CATCCCAGCC GCTCTCCCCT 
CCTTCAGACC TCTTGAGTCC CTGCGATGCC AAAGCTCTAG TATCGGGTCA GTGCTTAGAA 
GGTGACGGTT TGGTCCGTAC TGTGCTGGCT GGGGTCGATG TCCAGAGAGG TCATGGTGTC 
ATCATGCTGA CGGAGGAACG TCAGATGGAG CTTAACACAG TAGCGGTGGA CACCTGGCAC 
GTCCAGGAGT TTGACGACAG TGAAATCCCA GTGGAGAGCA ACGGACAGCT TTATGAAGGA 
GACTCTTATG TAATCCGCTG GAAGTACAGC ATCAGTACAG CTGACAGCAC AGACAGCTCA 
GATGAGTGTG GGACGGATCC AGCACCAAAG GAAAAGACGG CCTTCTTCCT GTGGCGAGGC 
CGTCACTCCA GTGTCAGTGG ACGGGACACA GCTGCTTTCC TTTCCATCGG GATGAGGAAC 
CACGAAGAGT CGCAGGTGGT TGTGCCTCAG GGAAAAGAGC CCCCCTGTTT CCTGCAGCTT 
TTCAAGGGAG GCTTGGTCAT TCACAAAGGA AAGCAAGAGG AAGCTTCCAT CAACACAGAA 
AGCTGGCGTC TGTTTTGTGT GCGAGGGGAG CTCCCAGAAG AGGGTTTTCT GTTAGAGGTG 
GACTGCTGCT GTGCGGGTCT GAGGTCGAGA GGCTGTGTTG TCCTGCTAAA TGGCCAGCAG 
GGAGTGCTCT ATCTCTGGAT TGGTTGTAAG GCTCACAGCA GCACAAAGGA AGTCGGCAAG 
AGGGTGGTGG AGGTCCTCAC TAAAATCTGT CCATCAGAGG TGGGTTTAAG CAAAAGCAGC 
CCTGTAAAGG TGCAGATAGT ACAGGAAGGT TCAGAGCCAG AAGACTTCTG GACAGCACTG 
GGACAAAAGG ACAGGAAGGC CTATGACTGC ATGCTGCAAG ATCCAGGAAA GTATAACTTC 
ACGCCTCGCC TCTTCCACCT GAGTGCCTCT TCTGGGAGTT TCCAGGCCGA GGAGCTGCAG 
AGTCCGATGC AGATGCCGGG GCTTGTGATG GCAATGCCTT TTGTCCAGGA GAGCCTATAC 
AGTGTACCAC AACCAGCTTT GTTCCTTCTT GACAACCGTC TGGAGGTCTA CTTGTGGCAG 
AGAGGTCAGC CTGAGCAGAC TGACAGCTCC TCAGCCTGGA AACTCTGGCA TCATGAGAGG 
AAGTGTGCCA TGCAGACAGC GCTGCAGTAC TGCAAAGAAA TAAACCAGAG AAGGCCGCCA 
CATGCGTATC TCATCCTCGA GGGGGCTGAG CCTCTCACCT TCACTAATGT CTTCCCGCGC 
TGGGAGAAAA GCCTTGAACC TCATGCACAG GGTGATACAG GGCGAGCGAA GCTGACCTTG 
GTGCAGGACA CCCTGGCCCA GCTCATGAAG ACCCAGTATC CTCTGGAGGA GCTGCTGCGG 
AGCCCCCTTC CTGAAGGAGT GGACCCCCAG CGCCTGGAGG TCTACCTGTC CAATGAGGAC 
TTTCAGACTA TTTTGGAAAT GAAGAGAGAT GAATATGCCT CCCTCCCAAG CTGGAAGCAA 
ATTGATCTGA AGAAGAGCAA AGGGCTGTTT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552441.2
CDS	307..5262
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEELQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552384.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552384.3

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGCT CCAGAAAGGC 
CCTGTTCAGC TTCCTGCCCT CTGTTTGTCC CCTCATCTGT CCGACAGACA GCAGGCCTCG 
TCTGTCAACC TGAAACCCAG TGAGGTACGT CTTTCACAGT CCACATCAGA TGCTGGCACT 
GGACTCGGAA AATCTGGTCC GACTCAACAG GGACCACAGT GCTACGACTC TGAGCCAGGC 
TTAAAAGGAA TCCTGAAGAA GAGTCGCTCT GCAGGCTCGG AGTGGAGCAG GACAGAAGCC 
AGTCAGGTAG ATGCATCATA CAGCCATGAA CAGAATGGTG GAGGCTGTAG AGAAGCGGCG 
ATACACGGGA GCAAAGCGGG AGGTGAGAGG GAGGGAACTT CTGCAGCACC ACAGAGAGAC 
AGACGAGAGA CCTCTGGTAT GGAGGAAGGT TCGCAGTCTG CTGCTCCCTG GAGGCAGAGG 
GCTAGAAACA GGAGGGAAAC CATTGCCTGC ACACCAATAA GAGCCTTGTC AGAGCAGGAT 
GCTCCACAGC AATCCTGCCA GTCCAAATAT GAACTGAGCT TTTCTTCTGA GAAAAACAGC 
GTAGACATCA ATGGAAGAGC CAAGCAGGAG GAAGACCAAA AGGCCATGAA CAAGATCCAT 
GACAACACCA CAGATATTAA AGCTGTCCGG GTCACCTCAG AAGAGTGTGC GCCTAAAATG 
CAGGAGGCAA CCAACGGCAG CAGAAGTGAG TATCATGTGC AGGAACAGTC AAAAAACCCT 
CATCTTGAGA ATGACAACCT TCAGTGCTGG GAGCCCGTCT TTGCCTCTGT CTATTCTATC 
AATACGCCCC AATATATCAT GTGTTTTAAT CAGACTAACC TCTCCTTTGA GGCACAAGAA 
GTCTCCTCTC CTACGAACAA TCAGAGTCAG CCTCAGTGGA GACATAAGGA TAAAGGAACT 
GAGATTCAGA TGACTGAATT TGGAGAATTG AAAACACAGC AGGAAAGCAA AGAGCAGGAG 
GCTGGAAGCA CGGCTAAGAC AAGCCTTGCA TCTGGTGACT CACAGAGAAA TGGTATCGCT 
CACAGCAGGA GACACTCCAC TGATGCTCCA TCATGCAAAA ATGAAGTGAC TCCACAGAGT 
TCTTCACCAA CAGTCACTAC TCAGGAGGTA AAGGAAGAGA CGCCTGTTGA TGAACCAAAC 
ATGTTTGATG GCAGTTTCTA CTGTGATCAG TCACCTTCTT CAGCCTGCCC CCCACCTTGT 
GGTGGAATTG CTGCCTCAGA GGGTGAGCAG GACCTGGGAG TCTTCTGCCA GACCAACACA 
CACATATTGA CCTCAGCAGT AGCAGAGCAC AGGCGGTCGG TACGTCCGTC CCGTCGGACT 
CAAGGCTCCA GAAACCCTCT GAGGGCTCTC GCAGCCAGAG AGGACATCAG GCAGGACTTC 
ATGGGAGAGA GAGTCAATTC ATCAGCTCAA GAGAGGATCC AAGCAGAAAA AAAGTCTAAG 
AACTCGTCTA TAGCAGACTC ACCTTCCTTA AGATCTGAAG AATCCTCCTT AGATGCCGAG 
ACAGCTAAGT CTTACCCGCC CTTCAGCAGC CTGATGCTAA TTCACATCAA AGGTAGGCGG 
CATGTTCAGG TCCGCCTGGT TGAGCCCTCA GTGCGGTCAC TGAACAGTGG AGACTGCTTC 
CTGCTGGTCA CTCCAGAGCA CTGCATCCTG TGGAGTGGAG AGTTTGCCAA CGGACAAGAA 
AAAGCAAAGG CCTCTGAGCT AGCTTTGTTC ATCCAGAACC AAAGGGACCT TGGTTGTTTG 
GCTTCCCACG TTGTCCACCT GCAGGAGGGC ATGAACTCTG ACAGCAGTGT GGCTGCAGAC 
TTCTGGAGTC TCCTGGGAGG AAGAACACAG TACAGAGGAG CTTGTGCTCC AGAGGAGGAT 
GAGCTCTATG AGCGCGGTGT GGTGGAGTCC AACTGTGTGT ACAGGCTGGT GGAAAACAAA 
CTGGTGCCTC ATGAACAGGC TTGGGCCTCC ATCCCCAGCA TCACCCTGTT GGCCTCCTCT 
GAGGCCCTGG TGTTTGATTT CGGCAGTGAG GTCTATCTGT GGCTCGGACA GGACGTTTCC 
CTCAGGAGGA GAAATGTTGC TCTCCAGTTG ACTCACCAGG TGTGGGTTGG AGCTTATGAC 
TACAGCAACT GTCAAGTAAA CCCACTGGAT CCCACTCAGT GTAACCCTGC AATTCAGCTA 
CGAGGAGAAG GCCGTCCCAG CTGGGCTCTG TTCGGCGTCC TCACTGAGAG CAACGAGACA 
GCTCTGTTCA GGGAGAAGTT CCTGGACTGG ATGTGCAGGG TTGGAGGCAG AGAGGAAGCG 
GCTTCAGGGA CGGATCAGAC GCAGTCCATC CCAGTCCAGT CATCCCAGCC GCTCTCCCCT 
CCTTCAGACC TCTTGAGTCC CTGCGATGCC AAAGCTCTAG TATCGGGTCA GTGCTTAGAA 
GGTGACGGTT TGGTCCGTAC TGTGCTGGCT GGGGTCGATG TCCAGAGAGG TCATGGTGTC 
ATCATGCTGA CGGAGGAACG TCAGATGGAG CTTAACACAG TAGCGGTGGA CACCTGGCAC 
GTCCAGGAGT TTGACGACAG TGAAATCCCA GTGGAGAGCA ACGGACAGCT TTATGAAGGA 
GACTCTTATG TAATCCGCTG GAAGTACAGC ATCAGTACAG CTGACAGCAC AGACAGCTCA 
GATGAGTGTG GGACGGATCC AGCACCAAAG GAAAAGACGG CCTTCTTCCT GTGGCGAGGC 
CGTCACTCCA GTGTCAGTGG ACGGGACACA GCTGCTTTCC TTTCCATCGG GATGAGGAAC 
CACGAAGAGT CGCAGGTGGT TGTGCCTCAG GGAAAAGAGC CCCCCTGTTT CCTGCAGCTT 
TTCAAGGGAG GCTTGGTCAT TCACAAAGGA AAGCAAGAGG AAGCTTCCAT CAACACAGAA 
AGCTGGCGTC TGTTTTGTGT GCGAGGGGAG CTCCCAGAAG AGGGTTTTCT GTTAGAGGTG 
GACTGCTGCT GTGCGGGTCT GAGGTCGAGA GGCTGTGTTG TCCTGCTAAA TGGCCAGCAG 
GGAGTGCTCT ATCTCTGGAT TGGTTGTAAG GCTCACAGCA GCACAAAGGA AGTCGGCAAG 
AGGGTGGTGG AGGTCCTCAC TAAAATCTGT CCATCAGAGG TGGGTTTAAG CAAAAGCAGC 
CCTGTAAAGG TGCAGATAGT ACAGGAAGGT TCAGAGCCAG AAGACTTCTG GACAGCACTG 
GGACAAAAGG ACAGGAAGGC CTATGACTGC ATGCTGCAAG ATCCAGGAAA GTATAACTTC 
ACGCCTCGCC TCTTCCACCT GAGTGCCTCT TCTGGGAGTT TCCAGGCCGA GGAGCTGCAG 
AGTCCGATGC AGATGCCGGG GCTTGTGATG GCAATGCCTT TTGTCCAGGA GAGCCTATAC 
AGTGTACCAC AACCAGCTTT GTTCCTTCTT GACAACCGTC TGGAGGTCTA CTTGTGGCAG 
AGAGGTCAGC CTGAGCAGAC TGACAGCTCC TCAGCCTGGA AACTCTGGCA TCATGAGAGG 
AAGTGTGCCA TGCAGACAGC GCTGCAGTAC TGCAAAGAAA TAAACCAGAG AAGGCCGCCA 
CATGCGTATC TCATCCTCGA GGGGGCTGAG CCTCTCACCT TCACTAATGT CTTCCCGCGC 
TGGGAGAAAA GCCTTGAACC TCATGCACAG GGTGATACAG GGCGAGCGAA GCTGACCTTG 
GTGCAGGACA CCCTGGCCCA GCTCATGAAG ACCCAGTATC CTCTGGAGGA GCTGCTGCGG 
AGCCCCCTTC CTGAAGGAGT GGACCCCCAG CGCCTGGAGG TCTACCTGTC CAATGAGGAC 
TTTCAGACTA TTTTGGAAAT GAAGAGAGAT GAATATGCCT CCCTCCCAAG CTGGAAGCAA 
ATTGATCTGA AGAAGAGCAA AGGGCTGTTT TGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552385.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552442.2 Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMa372242
Clone ID Related Accession (Same CDS sequence)	XM_004552385.3
Accession Version	XM_004552385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552442.2
CDS	307..5208
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKASFPDAGQATSSLDLAVNPGSEGGRRRTRRYLPSSSGGNRKTSERFRTQPITANEMEESSGCLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552385.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552385.3

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCTAGCT TCCCTGATGC TGGGCAAGCC ACATCCTCTC TGGATCTGGC TGTAAACCCG 
GGCTCTGAGG GGGGTCGGCG ACGCACTCGG CGTTACCTCC CTAGCTCGTC AGGCGGTAAC 
AGAAAAACTA GCGAACGCTT CAGGACACAG CCAATCACAG CCAATGAGAT GGAGGAGAGC 
AGTGGGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_004552386.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552443.2 Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.

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CloneID	OMa372243
Clone ID Related Accession (Same CDS sequence)	XM_004552386.3
Accession Version	XM_004552386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004552443.2
CDS	307..5091
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKACLDPENEENSKADVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_004552386.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004552386.3

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG CAGATGTAAA AACAGATGAC 
AGAGCCAAAA TGAGTGTGGC AGCCAAGATG TCTCTGTTTA AAGAGCTGGA GAAGTCTTCA 
GCCTCTGAGG CCTCGGCATT CCTCAAGCCT CGCTCAGGCT GCGTCTCTCA TGAACGCAGG 
AGCCGGCGTG GCAACGACCA TCGCTTTCTG ACACAGCCAA TCACCTGTGG AGAAATGGTG 
GCGATCAGCA CTCCCAAACC AGCACCACCA GTGGAGGTCC AGTCTGTGCA GGCTGAGTCA 
GAGGAGGATG GCGACGAGAG CTGCAAGCTG AGCATGAGCG AGAAGCTGGC CCTCTTCAAC 
AAACTGTCCC TGCCTGGGAG GGAGGGAGCC GGGCCTGCTG ACGGGCCCCC AGAGAGACGA 
AGGCAAAAGG GGGCTCGGTA CCGCACGCAG CCCATCACAG TGGAGGAGGT CAGTCTGCTC 
CAGAAAGGCC CTGTTCAGCT TCCTGCCCTC TGTTTGTCCC CTCATCTGTC CGACAGACAG 
CAGGCCTCGT CTGTCAACCT GAAACCCAGT GAGGTACGTC TTTCACAGTC CACATCAGAT 
GCTGGCACTG GACTCGGAAA ATCTGGTCCG ACTCAACAGG GACCACAGTG CTACGACTCT 
GAGCCAGGCT TAAAAGGAAT CCTGAAGAAG AGTCGCTCTG CAGGCTCGGA GTGGAGCAGG 
ACAGAAGCCA GTCAGGTAGA TGCATCATAC AGCCATGAAC AGAATGGTGG AGGCTGTAGA 
GAAGCGGCGA TACACGGGAG CAAAGCGGGA GGTGAGAGGG AGGGAACTTC TGCAGCACCA 
CAGAGAGACA GACGAGAGAC CTCTGGTATG GAGGAAGGTT CGCAGTCTGC TGCTCCCTGG 
AGGCAGAGGG CTAGAAACAG GAGGGAAACC ATTGCCTGCA CACCAATAAG AGCCTTGTCA 
GAGCAGGATG CTCCACAGCA ATCCTGCCAG TCCAAATATG AACTGAGCTT TTCTTCTGAG 
AAAAACAGCG TAGACATCAA TGGAAGAGCC AAGCAGGAGG AAGACCAAAA GGCCATGAAC 
AAGATCCATG ACAACACCAC AGATATTAAA GCTGTCCGGG TCACCTCAGA AGAGTGTGCG 
CCTAAAATGC AGGAGGCAAC CAACGGCAGC AGAAGTGAGT ATCATGTGCA GGAACAGTCA 
AAAAACCCTC ATCTTGAGAA TGACAACCTT CAGTGCTGGG AGCCCGTCTT TGCCTCTGTC 
TATTCTATCA ATACGCCCCA ATATATCATG TGTTTTAATC AGACTAACCT CTCCTTTGAG 
GCACAAGAAG TCTCCTCTCC TACGAACAAT CAGAGTCAGC CTCAGTGGAG ACATAAGGAT 
AAAGGAACTG AGATTCAGAT GACTGAATTT GGAGAATTGA AAACACAGCA GGAAAGCAAA 
GAGCAGGAGG CTGGAAGCAC GGCTAAGACA AGCCTTGCAT CTGGTGACTC ACAGAGAAAT 
GGTATCGCTC ACAGCAGGAG ACACTCCACT GATGCTCCAT CATGCAAAAA TGAAGTGACT 
CCACAGAGTT CTTCACCAAC AGTCACTACT CAGGAGGTAA AGGAAGAGAC GCCTGTTGAT 
GAACCAAACA TGTTTGATGG CAGTTTCTAC TGTGATCAGT CACCTTCTTC AGCCTGCCCC 
CCACCTTGTG GTGGAATTGC TGCCTCAGAG GGTGAGCAGG ACCTGGGAGT CTTCTGCCAG 
ACCAACACAC ACATATTGAC CTCAGCAGTA GCAGAGCACA GGCGGTCGGT ACGTCCGTCC 
CGTCGGACTC AAGGCTCCAG AAACCCTCTG AGGGCTCTCG CAGCCAGAGA GGACATCAGG 
CAGGACTTCA TGGGAGAGAG AGTCAATTCA TCAGCTCAAG AGAGGATCCA AGCAGAAAAA 
AAGTCTAAGA ACTCGTCTAT AGCAGACTCA CCTTCCTTAA GATCTGAAGA ATCCTCCTTA 
GATGCCGAGA CAGCTAAGTC TTACCCGCCC TTCAGCAGCC TGATGCTAAT TCACATCAAA 
GGTAGGCGGC ATGTTCAGGT CCGCCTGGTT GAGCCCTCAG TGCGGTCACT GAACAGTGGA 
GACTGCTTCC TGCTGGTCAC TCCAGAGCAC TGCATCCTGT GGAGTGGAGA GTTTGCCAAC 
GGACAAGAAA AAGCAAAGGC CTCTGAGCTA GCTTTGTTCA TCCAGAACCA AAGGGACCTT 
GGTTGTTTGG CTTCCCACGT TGTCCACCTG CAGGAGGGCA TGAACTCTGA CAGCAGTGTG 
GCTGCAGACT TCTGGAGTCT CCTGGGAGGA AGAACACAGT ACAGAGGAGC TTGTGCTCCA 
GAGGAGGATG AGCTCTATGA GCGCGGTGTG GTGGAGTCCA ACTGTGTGTA CAGGCTGGTG 
GAAAACAAAC TGGTGCCTCA TGAACAGGCT TGGGCCTCCA TCCCCAGCAT CACCCTGTTG 
GCCTCCTCTG AGGCCCTGGT GTTTGATTTC GGCAGTGAGG TCTATCTGTG GCTCGGACAG 
GACGTTTCCC TCAGGAGGAG AAATGTTGCT CTCCAGTTGA CTCACCAGGT GTGGGTTGGA 
GCTTATGACT ACAGCAACTG TCAAGTAAAC CCACTGGATC CCACTCAGTG TAACCCTGCA 
ATTCAGCTAC GAGGAGAAGG CCGTCCCAGC TGGGCTCTGT TCGGCGTCCT CACTGAGAGC 
AACGAGACAG CTCTGTTCAG GGAGAAGTTC CTGGACTGGA TGTGCAGGGT TGGAGGCAGA 
GAGGAAGCGG CTTCAGGGAC GGATCAGACG CAGTCCATCC CAGTCCAGTC ATCCCAGCCG 
CTCTCCCCTC CTTCAGACCT CTTGAGTCCC TGCGATGCCA AAGCTCTAGT ATCGGGTCAG 
TGCTTAGAAG GTGACGGTTT GGTCCGTACT GTGCTGGCTG GGGTCGATGT CCAGAGAGGT 
CATGGTGTCA TCATGCTGAC GGAGGAACGT CAGATGGAGC TTAACACAGT AGCGGTGGAC 
ACCTGGCACG TCCAGGAGTT TGACGACAGT GAAATCCCAG TGGAGAGCAA CGGACAGCTT 
TATGAAGGAG ACTCTTATGT AATCCGCTGG AAGTACAGCA TCAGTACAGC TGACAGCACA 
GACAGCTCAG ATGAGTGTGG GACGGATCCA GCACCAAAGG AAAAGACGGC CTTCTTCCTG 
TGGCGAGGCC GTCACTCCAG TGTCAGTGGA CGGGACACAG CTGCTTTCCT TTCCATCGGG 
ATGAGGAACC ACGAAGAGTC GCAGGTGGTT GTGCCTCAGG GAAAAGAGCC CCCCTGTTTC 
CTGCAGCTTT TCAAGGGAGG CTTGGTCATT CACAAAGGAA AGCAAGAGGA AGCTTCCATC 
AACACAGAAA GCTGGCGTCT GTTTTGTGTG CGAGGGGAGC TCCCAGAAGA GGGTTTTCTG 
TTAGAGGTGG ACTGCTGCTG TGCGGGTCTG AGGTCGAGAG GCTGTGTTGT CCTGCTAAAT 
GGCCAGCAGG GAGTGCTCTA TCTCTGGATT GGTTGTAAGG CTCACAGCAG CACAAAGGAA 
GTCGGCAAGA GGGTGGTGGA GGTCCTCACT AAAATCTGTC CATCAGAGGT GGGTTTAAGC 
AAAAGCAGCC CTGTAAAGGT GCAGATAGTA CAGGAAGGTT CAGAGCCAGA AGACTTCTGG 
ACAGCACTGG GACAAAAGGA CAGGAAGGCC TATGACTGCA TGCTGCAAGA TCCAGGAAAG 
TATAACTTCA CGCCTCGCCT CTTCCACCTG AGTGCCTCTT CTGGGAGTTT CCAGGCCGAG 
GAGCTGCAGA GTCCGATGCA GATGCCGGGG CTTGTGATGG CAATGCCTTT TGTCCAGGAG 
AGCCTATACA GTGTACCACA ACCAGCTTTG TTCCTTCTTG ACAACCGTCT GGAGGTCTAC 
TTGTGGCAGA GAGGTCAGCC TGAGCAGACT GACAGCTCCT CAGCCTGGAA ACTCTGGCAT 
CATGAGAGGA AGTGTGCCAT GCAGACAGCG CTGCAGTACT GCAAAGAAAT AAACCAGAGA 
AGGCCGCCAC ATGCGTATCT CATCCTCGAG GGGGCTGAGC CTCTCACCTT CACTAATGTC 
TTCCCGCGCT GGGAGAAAAG CCTTGAACCT CATGCACAGG GTGATACAGG GCGAGCGAAG 
CTGACCTTGG TGCAGGACAC CCTGGCCCAG CTCATGAAGA CCCAGTATCC TCTGGAGGAG 
CTGCTGCGGA GCCCCCTTCC TGAAGGAGTG GACCCCCAGC GCCTGGAGGT CTACCTGTCC 
AATGAGGACT TTCAGACTAT TTTGGAAATG AAGAGAGATG AATATGCCTC CCTCCCAAGC 
TGGAAGCAAA TTGATCTGAA GAAGAGCAAA GGGCTGTTTT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101479339 ( XM_023152523.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008291.2 Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.

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CloneID	OMa372244
Clone ID Related Accession (Same CDS sequence)	XM_023152523.2
Accession Version	XM_023152523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036783.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023152523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC TTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGGCAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008291.2
CDS	307..5088
Translation	MMENAVLEPRSERIARYKAERRRELAERFGSIDELPSKWVRRDEKKVHEPASQAHGGMLSSHDLSEKVNSRTQDVTNGLETPAEPDCLRRQGSQNSASMQSGEEPHVPHGLDGPQLHTRVSVGQLRSALLQQTESGAQPEKACLDPENEENSKDVKTDDRAKMSVAAKMSLFKELEKSSASEASAFLKPRSGCVSHERRSRRGNDHRFLTQPITCGEMVAISTPKPAPPVEVQSVQAESEEDGDESCKLSMSEKLALFNKLSLPGREGAGPADGPPERRRQKGARYRTQPITVEEVSLLQKGPVQLPALCLSPHLSDRQQASSVNLKPSEVRLSQSTSDAGTGLGKSGPTQQGPQCYDSEPGLKGILKKSRSAGSEWSRTEASQVDASYSHEQNGGGCREAAIHGSKAGGEREGTSAAPQRDRRETSGMEEGSQSAAPWRQRARNRRETIACTPIRALSEQDAPQQSCQSKYELSFSSEKNSVDINGRAKQEEDQKAMNKIHDNTTDIKAVRVTSEECAPKMQEATNGSRSEYHVQEQSKNPHLENDNLQCWEPVFASVYSINTPQYIMCFNQTNLSFEAQEVSSPTNNQSQPQWRHKDKGTEIQMTEFGELKTQQESKEQEAGSTAKTSLASGDSQRNGIAHSRRHSTDAPSCKNEVTPQSSSPTVTTQEVKEETPVDEPNMFDGSFYCDQSPSSACPPPCGGIAASEGEQDLGVFCQTNTHILTSAVAEHRRSVRPSRRTQGSRNPLRALAAREDIRQDFMGERVNSSAQERIQAEKKSKNSSIADSPSLRSEESSLDAETAKSYPPFSSLMLIHIKGRRHVQVRLVEPSVRSLNSGDCFLLVTPEHCILWSGEFANGQEKAKASELALFIQNQRDLGCLASHVVHLQEGMNSDSSVAADFWSLLGGRTQYRGACAPEEDELYERGVVESNCVYRLVENKLVPHEQAWASIPSITLLASSEALVFDFGSEVYLWLGQDVSLRRRNVALQLTHQVWVGAYDYSNCQVNPLDPTQCNPAIQLRGEGRPSWALFGVLTESNETALFREKFLDWMCRVGGREEAASGTDQTQSIPVQSSQPLSPPSDLLSPCDAKALVSGQCLEGDGLVRTVLAGVDVQRGHGVIMLTEERQMELNTVAVDTWHVQEFDDSEIPVESNGQLYEGDSYVIRWKYSISTADSTDSSDECGTDPAPKEKTAFFLWRGRHSSVSGRDTAAFLSIGMRNHEESQVVVPQGKEPPCFLQLFKGGLVIHKGKQEEASINTESWRLFCVRGELPEEGFLLEVDCCCAGLRSRGCVVLLNGQQGVLYLWIGCKAHSSTKEVGKRVVEVLTKICPSEVGLSKSSPVKVQIVQEGSEPEDFWTALGQKDRKAYDCMLQDPGKYNFTPRLFHLSASSGSFQAEELQSPMQMPGLVMAMPFVQESLYSVPQPALFLLDNRLEVYLWQRGQPEQTDSSSAWKLWHHERKCAMQTALQYCKEINQRRPPHAYLILEGAEPLTFTNVFPRWEKSLEPHAQGDTGRAKLTLVQDTLAQLMKTQYPLEELLRSPLPEGVDPQRLEVYLSNEDFQTILEMKRDEYASLPSWKQIDLKKSKGLFC

Target ORF information:

RefSeq Version	XM_023152523.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra supervillin (LOC101479339), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152523.2

ORF Insert Sequence:

ATGATGGAGA ACGCCGTCCT GGAGCCCAGA TCGGAGCGGA TTGCCCGCTA CAAGGCTGAG 
AGGAGGCGAG AACTGGCCGA ACGCTTTGGC AGCATTGACG AGCTTCCTTC TAAGTGGGTG 
AGAAGGGATG AGAAAAAGGT TCATGAGCCA GCGAGCCAAG CGCATGGAGG AATGTTAAGC 
TCCCATGATC TGAGTGAAAA AGTGAACAGC AGAACACAGG ATGTTACAAA TGGATTAGAG 
ACTCCTGCTG AGCCCGACTG CCTGAGAAGG CAGGGTTCCC AGAACTCTGC TAGCATGCAG 
AGTGGGGAAG AGCCTCATGT CCCTCATGGA CTCGACGGCC CACAGCTCCA CACTCGGGTG 
TCCGTGGGCC AGTTGAGAAG TGCTCTTCTA CAGCAGACGG AGAGCGGAGC ACAGCCTGAG 
AAAGCGTGCT TGGATCCAGA GAACGAGGAA AACAGCAAAG ATGTAAAAAC AGATGACAGA 
GCCAAAATGA GTGTGGCAGC CAAGATGTCT CTGTTTAAAG AGCTGGAGAA GTCTTCAGCC 
TCTGAGGCCT CGGCATTCCT CAAGCCTCGC TCAGGCTGCG TCTCTCATGA ACGCAGGAGC 
CGGCGTGGCA ACGACCATCG CTTTCTGACA CAGCCAATCA CCTGTGGAGA AATGGTGGCG 
ATCAGCACTC CCAAACCAGC ACCACCAGTG GAGGTCCAGT CTGTGCAGGC TGAGTCAGAG 
GAGGATGGCG ACGAGAGCTG CAAGCTGAGC ATGAGCGAGA AGCTGGCCCT CTTCAACAAA 
CTGTCCCTGC CTGGGAGGGA GGGAGCCGGG CCTGCTGACG GGCCCCCAGA GAGACGAAGG 
CAAAAGGGGG CTCGGTACCG CACGCAGCCC ATCACAGTGG AGGAGGTCAG TCTGCTCCAG 
AAAGGCCCTG TTCAGCTTCC TGCCCTCTGT TTGTCCCCTC ATCTGTCCGA CAGACAGCAG 
GCCTCGTCTG TCAACCTGAA ACCCAGTGAG GTACGTCTTT CACAGTCCAC ATCAGATGCT 
GGCACTGGAC TCGGAAAATC TGGTCCGACT CAACAGGGAC CACAGTGCTA CGACTCTGAG 
CCAGGCTTAA AAGGAATCCT GAAGAAGAGT CGCTCTGCAG GCTCGGAGTG GAGCAGGACA 
GAAGCCAGTC AGGTAGATGC ATCATACAGC CATGAACAGA ATGGTGGAGG CTGTAGAGAA 
GCGGCGATAC ACGGGAGCAA AGCGGGAGGT GAGAGGGAGG GAACTTCTGC AGCACCACAG 
AGAGACAGAC GAGAGACCTC TGGTATGGAG GAAGGTTCGC AGTCTGCTGC TCCCTGGAGG 
CAGAGGGCTA GAAACAGGAG GGAAACCATT GCCTGCACAC CAATAAGAGC CTTGTCAGAG 
CAGGATGCTC CACAGCAATC CTGCCAGTCC AAATATGAAC TGAGCTTTTC TTCTGAGAAA 
AACAGCGTAG ACATCAATGG AAGAGCCAAG CAGGAGGAAG ACCAAAAGGC CATGAACAAG 
ATCCATGACA ACACCACAGA TATTAAAGCT GTCCGGGTCA CCTCAGAAGA GTGTGCGCCT 
AAAATGCAGG AGGCAACCAA CGGCAGCAGA AGTGAGTATC ATGTGCAGGA ACAGTCAAAA 
AACCCTCATC TTGAGAATGA CAACCTTCAG TGCTGGGAGC CCGTCTTTGC CTCTGTCTAT 
TCTATCAATA CGCCCCAATA TATCATGTGT TTTAATCAGA CTAACCTCTC CTTTGAGGCA 
CAAGAAGTCT CCTCTCCTAC GAACAATCAG AGTCAGCCTC AGTGGAGACA TAAGGATAAA 
GGAACTGAGA TTCAGATGAC TGAATTTGGA GAATTGAAAA CACAGCAGGA AAGCAAAGAG 
CAGGAGGCTG GAAGCACGGC TAAGACAAGC CTTGCATCTG GTGACTCACA GAGAAATGGT 
ATCGCTCACA GCAGGAGACA CTCCACTGAT GCTCCATCAT GCAAAAATGA AGTGACTCCA 
CAGAGTTCTT CACCAACAGT CACTACTCAG GAGGTAAAGG AAGAGACGCC TGTTGATGAA 
CCAAACATGT TTGATGGCAG TTTCTACTGT GATCAGTCAC CTTCTTCAGC CTGCCCCCCA 
CCTTGTGGTG GAATTGCTGC CTCAGAGGGT GAGCAGGACC TGGGAGTCTT CTGCCAGACC 
AACACACACA TATTGACCTC AGCAGTAGCA GAGCACAGGC GGTCGGTACG TCCGTCCCGT 
CGGACTCAAG GCTCCAGAAA CCCTCTGAGG GCTCTCGCAG CCAGAGAGGA CATCAGGCAG 
GACTTCATGG GAGAGAGAGT CAATTCATCA GCTCAAGAGA GGATCCAAGC AGAAAAAAAG 
TCTAAGAACT CGTCTATAGC AGACTCACCT TCCTTAAGAT CTGAAGAATC CTCCTTAGAT 
GCCGAGACAG CTAAGTCTTA CCCGCCCTTC AGCAGCCTGA TGCTAATTCA CATCAAAGGT 
AGGCGGCATG TTCAGGTCCG CCTGGTTGAG CCCTCAGTGC GGTCACTGAA CAGTGGAGAC 
TGCTTCCTGC TGGTCACTCC AGAGCACTGC ATCCTGTGGA GTGGAGAGTT TGCCAACGGA 
CAAGAAAAAG CAAAGGCCTC TGAGCTAGCT TTGTTCATCC AGAACCAAAG GGACCTTGGT 
TGTTTGGCTT CCCACGTTGT CCACCTGCAG GAGGGCATGA ACTCTGACAG CAGTGTGGCT 
GCAGACTTCT GGAGTCTCCT GGGAGGAAGA ACACAGTACA GAGGAGCTTG TGCTCCAGAG 
GAGGATGAGC TCTATGAGCG CGGTGTGGTG GAGTCCAACT GTGTGTACAG GCTGGTGGAA 
AACAAACTGG TGCCTCATGA ACAGGCTTGG GCCTCCATCC CCAGCATCAC CCTGTTGGCC 
TCCTCTGAGG CCCTGGTGTT TGATTTCGGC AGTGAGGTCT ATCTGTGGCT CGGACAGGAC 
GTTTCCCTCA GGAGGAGAAA TGTTGCTCTC CAGTTGACTC ACCAGGTGTG GGTTGGAGCT 
TATGACTACA GCAACTGTCA AGTAAACCCA CTGGATCCCA CTCAGTGTAA CCCTGCAATT 
CAGCTACGAG GAGAAGGCCG TCCCAGCTGG GCTCTGTTCG GCGTCCTCAC TGAGAGCAAC 
GAGACAGCTC TGTTCAGGGA GAAGTTCCTG GACTGGATGT GCAGGGTTGG AGGCAGAGAG 
GAAGCGGCTT CAGGGACGGA TCAGACGCAG TCCATCCCAG TCCAGTCATC CCAGCCGCTC 
TCCCCTCCTT CAGACCTCTT GAGTCCCTGC GATGCCAAAG CTCTAGTATC GGGTCAGTGC 
TTAGAAGGTG ACGGTTTGGT CCGTACTGTG CTGGCTGGGG TCGATGTCCA GAGAGGTCAT 
GGTGTCATCA TGCTGACGGA GGAACGTCAG ATGGAGCTTA ACACAGTAGC GGTGGACACC 
TGGCACGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTGG AGAGCAACGG ACAGCTTTAT 
GAAGGAGACT CTTATGTAAT CCGCTGGAAG TACAGCATCA GTACAGCTGA CAGCACAGAC 
AGCTCAGATG AGTGTGGGAC GGATCCAGCA CCAAAGGAAA AGACGGCCTT CTTCCTGTGG 
CGAGGCCGTC ACTCCAGTGT CAGTGGACGG GACACAGCTG CTTTCCTTTC CATCGGGATG 
AGGAACCACG AAGAGTCGCA GGTGGTTGTG CCTCAGGGAA AAGAGCCCCC CTGTTTCCTG 
CAGCTTTTCA AGGGAGGCTT GGTCATTCAC AAAGGAAAGC AAGAGGAAGC TTCCATCAAC 
ACAGAAAGCT GGCGTCTGTT TTGTGTGCGA GGGGAGCTCC CAGAAGAGGG TTTTCTGTTA 
GAGGTGGACT GCTGCTGTGC GGGTCTGAGG TCGAGAGGCT GTGTTGTCCT GCTAAATGGC 
CAGCAGGGAG TGCTCTATCT CTGGATTGGT TGTAAGGCTC ACAGCAGCAC AAAGGAAGTC 
GGCAAGAGGG TGGTGGAGGT CCTCACTAAA ATCTGTCCAT CAGAGGTGGG TTTAAGCAAA 
AGCAGCCCTG TAAAGGTGCA GATAGTACAG GAAGGTTCAG AGCCAGAAGA CTTCTGGACA 
GCACTGGGAC AAAAGGACAG GAAGGCCTAT GACTGCATGC TGCAAGATCC AGGAAAGTAT 
AACTTCACGC CTCGCCTCTT CCACCTGAGT GCCTCTTCTG GGAGTTTCCA GGCCGAGGAG 
CTGCAGAGTC CGATGCAGAT GCCGGGGCTT GTGATGGCAA TGCCTTTTGT CCAGGAGAGC 
CTATACAGTG TACCACAACC AGCTTTGTTC CTTCTTGACA ACCGTCTGGA GGTCTACTTG 
TGGCAGAGAG GTCAGCCTGA GCAGACTGAC AGCTCCTCAG CCTGGAAACT CTGGCATCAT 
GAGAGGAAGT GTGCCATGCA GACAGCGCTG CAGTACTGCA AAGAAATAAA CCAGAGAAGG 
CCGCCACATG CGTATCTCAT CCTCGAGGGG GCTGAGCCTC TCACCTTCAC TAATGTCTTC 
CCGCGCTGGG AGAAAAGCCT TGAACCTCAT GCACAGGGTG ATACAGGGCG AGCGAAGCTG 
ACCTTGGTGC AGGACACCCT GGCCCAGCTC ATGAAGACCC AGTATCCTCT GGAGGAGCTG 
CTGCGGAGCC CCCTTCCTGA AGGAGTGGAC CCCCAGCGCC TGGAGGTCTA CCTGTCCAAT 
GAGGACTTTC AGACTATTTT GGAAATGAAG AGAGATGAAT ATGCCTCCCT CCCAAGCTGG 
AAGCAAATTG ATCTGAAGAA GAGCAAAGGG CTGTTTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC101479339
Entrez Gene ID	101479339
Full Name	supervillin
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

LOC101479339 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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