cps1 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	cps1
Entrez Gene ID	101479159
Full Name	carbamoyl-phosphate synthase 1
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014408282.1	XP_014263768.1	carbamoyl-phosphate synthase [ammonia], mitochondrial
XM_014408283.1	XP_014263769.1	carbamoyl-phosphate synthase [ammonia], mitochondrial
XM_023152633.1	XP_023008401.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
XM_014408283.2	XP_014263769.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_014408282.2	XP_014263768.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
XM_014408284.1	XP_014263770.1	carbamoyl-phosphate synthase [ammonia], mitochondrial
XM_014408284.2	XP_014263770.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following cps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa312454	XM_023152633.1 Latest version!	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMa35385	XM_014408284.2 Latest version!	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa35385	XM_014408283.2 Latest version!	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa35385	XM_014408282.2 Latest version!	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMa35385	XM_014408283.1	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa35385	XM_014408282.1	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa35385	XM_014408284.1	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cps1 ( XM_023152633.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008401.1 Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMa312454
Clone ID Related Accession (Same CDS sequence)	XM_023152633.1
Accession Version	XM_023152633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036795.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GGTGCAGAGA GAATGGATTG GATTATAGAC 
AAAATGAGTT TTAAAAAAAG AAAGCATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008401.1
CDS	194..4186
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKGAERMDWIIDKMSFKKRKHK

Target ORF information:

RefSeq Version	XM_023152633.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152633.1

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GGTGCAGAGA GAATGGATTG GATTATAGAC 
AAAATGAGTT TTAAAAAAAG AAAGCATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408282.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263768.1 Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408282.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447828.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263768.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408282.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408282.1

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408283.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263769.1 Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X2, mRNA.

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CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408283.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447828.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263769.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408283.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408283.1

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408283.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263769.1 Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X2, mRNA.

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CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036795.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263769.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408283.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408283.2

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408282.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263768.1 Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036795.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263768.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408282.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408282.2

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408284.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263770.1 Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408284.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447828.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263770.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408284.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1, mitochondrial (cps1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408284.1

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cps1 ( XM_014408284.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263770.1 Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X3, mRNA.

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CloneID	OMa35385
Clone ID Related Accession (Same CDS sequence)	XM_014408282.1 , XM_014408283.1 , XM_014408283.2 , XM_014408282.2 , XM_014408284.1 , XM_014408284.2
Accession Version	XM_014408284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036795.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263770.1
CDS	194..4714
Translation	MAKILQAVSVVKSLRQGCVLGRNVGNAGCLRLHTAKLFSVKAQTAHLVLEDGTRMKGISFGHDASVAGELVFNTGLVGYPEALTDPSYRGQILTLTYPIVGNYGVPNTEELDELGLRKHVESERIQVSGLLVQDYSHEYSHWNSVKSLGQWLQEEKVPALFGIDTRMLTKVIRDKGTVLGKIEFDGQPVDITDPNQKNLVAEVSTKEVKVFGKGNPIKVVAVDCGIKHNIIRLLVKRGAEVHLVPWDQDLLSLDYDGLFISNGPGDPSLAKTLINNVHKVLESDRPQPVFGICMGNQITALAAGAKSYKLPMGNRGQNQPVLNVMTGQAFITAQNHGYGIDSESLPPGWGPLFINANDGTNEGVMHSTKPVFTAQFHPEAKGGPTDTEFLFDVFLSLIKKGKNISIASVMPIKPRIPPRAQVSKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENLKTVLMNPNIASVQTNEVGSKQADSVYFLPVTPEFVTEVIKTERPDGILLSMGGQTALNCGVELFQRGILDQYGVKVLGTPVESIMATEDRQLFADKLMEINEKIAPSIAVESVSDALRAAEQIGYPVMLRSAYALGGLGSGLCANKEKLEETAQKALAMSNQILVEKSLLGWKEVEYEVVRDVADNCVTVCNMENFDPLGIHTGDSIVVAPSQTLSNEEYHMLRETAIKVVRHLGIVGECNIQYALHPSSLEYCIIEVNARLSRSSALASKATGYPLAFVAAKLALGIPLPEIKNAVSEKTTACFEPSLDYIVTKIPRWDLDRFHGMSREIGSAMKSVGEVMAIGRTFEESMQKALRMCHPSVDGFVPRLPLKKPWSDTHDLEQELAVPSSTRIFSLAKALYSGMMSVDQIHQLTAIDKWFLHKLHGITNVEQHLANYNSGTVPKGMLLKAKQDGFSDRQVGQILGCNEGVARELRLNYGIRPWVKQIDTLAAEYPAMTNYLYCTYHGQEHDLDFKDQGIMVLGCGPYHIGSSVEFDWCAVSSIRALRQMGKKTVVVNHNPETVSTDFDECDRLYFEELTLERILDITQQEGCSGCIVSVGGQIPNNLAVPLHMSGVKILGTSPLQIDRAEERSVFSSILDDLEVAQAPWRALSSLEDAFAFANQMGYPCLLRPSYVLSGSAMNVVYGEEEMKRFLEEAAHVSQEHPVVITKFIRGAREVEVDAVAKNGKVLVHAITEHVEDAGVHSGDATLMLPTQTISQGAQEKVKIATRKIAHAFEISGPFNTQFLVKGNDVMVIECNLRASRSFPFVSKTIGVDFINVATKVMVGEPLDEASLPSLDKPIIPVHYVGIKAPMFSWPRLRDADPVLRCEMASTGEVACFGPNIYSAFLKAMLSTGFKLPQKGILIGIQHSFRPSFLATANQLKEEGFKLYATEATSAWLCANDVPAIPVAWPTGMGGDNSLPSIKRLISERHIDLVVNLPNNNTRHVKDNFLIRRMAIDHGVPLITNFQVVKLFAEAIHYAAQLDTTSLFHYRQKESQQRGSGQQG

Target ORF information:

RefSeq Version	XM_014408284.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra carbamoyl-phosphate synthase 1 (cps1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408284.2

ORF Insert Sequence:

ATGGCAAAAA TCCTCCAAGC TGTCAGCGTT GTGAAATCTT TGAGACAAGG ATGCGTGTTA 
GGCAGAAACG TGGGGAACGC GGGCTGCCTG AGACTTCACA CTGCAAAACT TTTTAGTGTC 
AAGGCCCAGA CAGCCCACCT GGTCTTGGAG GATGGCACCA GGATGAAAGG GATTTCTTTT 
GGGCATGATG CCTCTGTTGC TGGAGAGCTG GTCTTCAATA CTGGTCTTGT GGGGTATCCC 
GAGGCCCTGA CTGACCCAAG TTATAGAGGT CAAATACTGA CGCTGACATA TCCTATTGTA 
GGGAACTATG GTGTGCCGAA TACAGAGGAA CTGGATGAGC TAGGCCTGAG AAAACATGTA 
GAGTCAGAAC GCATTCAAGT ATCTGGTCTA CTGGTTCAAG ACTACAGCCA CGAGTACAGC 
CACTGGAACT CTGTCAAGTC TCTGGGTCAA TGGCTGCAAG AAGAGAAGGT TCCTGCACTG 
TTTGGGATAG ACACCAGGAT GCTGACCAAA GTCATCAGAG ATAAGGGAAC AGTTTTGGGG 
AAGATTGAAT TTGATGGTCA GCCTGTTGAC ATAACTGACC CCAATCAGAA AAACTTAGTA 
GCAGAAGTTT CAACCAAGGA AGTCAAAGTT TTTGGAAAAG GCAACCCAAT TAAAGTGGTG 
GCTGTCGACT GTGGCATTAA ACATAACATA ATTCGACTGC TGGTTAAGCG AGGTGCAGAA 
GTCCACCTAG TGCCGTGGGA CCAGGACTTG CTTAGTCTAG ACTATGATGG CCTGTTCATA 
TCTAATGGAC CAGGAGATCC ATCTCTGGCC AAGACACTCA TCAATAACGT GCACAAGGTG 
CTGGAGAGCG ATCGTCCCCA GCCAGTATTT GGGATCTGTA TGGGCAATCA GATCACTGCC 
CTGGCTGCAG GAGCCAAGTC ATACAAACTA CCTATGGGCA ACAGAGGCCA GAACCAGCCG 
GTGTTGAATG TGATGACGGG CCAGGCCTTC ATTACCGCAC AGAACCACGG TTATGGCATA 
GACAGCGAGT CCCTGCCCCC AGGGTGGGGC CCGCTTTTCA TCAACGCAAA TGATGGCACC 
AATGAGGGGG TCATGCACAG CACTAAACCT GTTTTTACAG CCCAGTTTCA CCCTGAGGCT 
AAGGGCGGTC CCACGGACAC AGAGTTTCTC TTTGATGTCT TTTTGTCCCT GATCAAAAAA 
GGAAAGAATA TCAGCATAGC GTCAGTCATG CCCATAAAAC CCAGGATCCC TCCCAGGGCC 
CAGGTTTCAA AGGTGCTTGT ATTGGGTTCT GGTGGCCTCT CTATTGGTCA GGCTGGTGAG 
TTTGACTACT CTGGATCTCA AGCCATCAAG GCCATGAAGG AGGAAAACCT GAAGACTGTG 
CTCATGAATC CAAACATAGC CTCCGTTCAG ACCAATGAAG TGGGCTCCAA GCAGGCTGAC 
TCAGTCTACT TCCTGCCTGT CACGCCAGAA TTTGTAACCG AGGTCATCAA GACAGAGCGT 
CCAGACGGCA TCCTGCTCTC GATGGGTGGA CAAACTGCAC TCAACTGTGG GGTAGAGTTA 
TTCCAGCGTG GGATCTTGGA CCAGTATGGG GTGAAAGTCC TTGGAACCCC TGTGGAGTCC 
ATAATGGCAA CAGAGGACAG GCAGCTTTTT GCTGACAAAC TAATGGAGAT CAATGAGAAA 
ATTGCACCTA GTATTGCTGT TGAGTCGGTG TCTGATGCAT TACGAGCAGC AGAGCAGATA 
GGCTACCCGG TGATGTTGCG ATCGGCCTAC GCTCTGGGGG GTCTGGGCTC TGGTCTGTGT 
GCCAATAAAG AAAAGCTGGA GGAAACTGCC CAGAAGGCTC TGGCCATGAG CAATCAAATC 
CTCGTGGAAA AGTCTTTGCT TGGCTGGAAA GAGGTGGAAT ACGAGGTGGT ACGAGACGTT 
GCTGATAACT GTGTCACCGT CTGCAACATG GAGAACTTCG ATCCACTGGG CATCCACACA 
GGTGACTCCA TTGTGGTGGC ACCAAGCCAG ACATTATCCA ATGAGGAATA CCATATGCTC 
CGTGAAACTG CTATCAAGGT GGTACGTCAC CTTGGCATTG TTGGCGAATG CAACATCCAG 
TATGCCTTAC ACCCATCTTC TTTGGAGTAC TGCATTATTG AGGTGAATGC ACGGCTATCG 
AGAAGCTCAG CTCTTGCATC CAAAGCAACT GGTTACCCCT TGGCATTTGT GGCTGCTAAG 
CTGGCTCTGG GAATCCCCCT GCCTGAGATC AAGAATGCTG TATCAGAGAA GACCACAGCC 
TGCTTTGAGC CCAGTTTAGA CTACATCGTC ACCAAGATTC CTCGCTGGGA CCTCGACCGC 
TTTCACGGCA TGTCTCGTGA AATAGGCAGT GCCATGAAGA GTGTAGGAGA GGTGATGGCA 
ATAGGCCGTA CGTTTGAGGA GAGTATGCAG AAGGCCTTAA GGATGTGCCA TCCATCAGTA 
GATGGCTTTG TGCCCCGGCT GCCACTCAAG AAGCCGTGGT CCGACACCCA CGATCTAGAA 
CAGGAGCTGG CTGTGCCCTC TAGCACCCGC ATCTTCTCCC TCGCTAAGGC CCTCTACAGT 
GGCATGATGA GTGTTGATCA GATCCACCAG CTGACAGCCA TAGACAAGTG GTTCCTCCAC 
AAACTTCATG GAATAACAAA CGTGGAGCAA CATCTTGCAA ACTACAACAG TGGCACAGTC 
CCCAAGGGGA TGTTGCTAAA GGCCAAGCAG GACGGCTTCT CAGACCGGCA GGTTGGTCAG 
ATTCTAGGCT GCAATGAGGG AGTAGCAAGA GAGCTGAGGC TCAATTATGG CATCAGACCT 
TGGGTCAAAC AGATTGACAC TTTGGCTGCA GAGTACCCAG CAATGACCAA CTACCTGTAT 
TGTACTTACC ACGGTCAGGA GCATGATCTG GACTTTAAAG ACCAGGGAAT TATGGTTCTT 
GGTTGTGGAC CCTACCACAT AGGCAGCAGT GTTGAGTTTG ACTGGTGTGC GGTGTCCAGC 
ATCAGAGCCT TGAGACAAAT GGGCAAGAAG ACAGTTGTAG TAAACCACAA CCCAGAGACG 
GTCAGCACAG ACTTCGATGA GTGTGATCGC CTTTACTTTG AAGAACTGAC CCTGGAACGC 
ATTCTGGATA TCACCCAGCA GGAGGGTTGC TCTGGCTGCA TCGTATCAGT GGGAGGTCAG 
ATTCCTAACA ACCTAGCCGT CCCCTTGCAC ATGAGTGGGG TGAAGATTCT CGGGACGAGC 
CCCCTGCAGA TCGACCGAGC TGAAGAGCGC TCAGTCTTCT CCAGTATCCT GGATGACCTC 
GAGGTTGCCC AAGCCCCATG GAGGGCCCTG AGCTCCCTGG AAGACGCGTT TGCCTTCGCT 
AACCAGATGG GGTACCCCTG TCTGCTGAGG CCCTCCTATG TTCTGAGTGG CTCTGCTATG 
AACGTTGTGT ACGGTGAAGA GGAGATGAAA CGCTTCTTGG AAGAGGCTGC ACACGTGTCC 
CAGGAGCATC CAGTTGTCAT CACCAAATTC ATACGTGGTG CCAGGGAGGT GGAGGTAGAT 
GCTGTGGCCA AGAATGGCAA GGTTTTGGTT CATGCCATAA CAGAGCATGT GGAAGATGCT 
GGGGTGCACT CTGGAGATGC TACCCTAATG CTGCCAACAC AGACCATTAG CCAAGGGGCA 
CAGGAAAAGG TTAAAATTGC CACCCGAAAA ATCGCGCACG CATTTGAAAT TTCTGGGCCA 
TTTAACACTC AATTTCTGGT TAAAGGCAAT GATGTCATGG TGATCGAATG CAATCTGCGA 
GCGTCACGCT CCTTCCCTTT TGTATCCAAA ACAATTGGTG TCGACTTTAT CAACGTGGCA 
ACCAAAGTGA TGGTGGGAGA GCCTTTGGAT GAGGCCAGCC TTCCCTCGCT CGATAAACCC 
ATCATTCCCG TGCACTACGT TGGAATCAAA GCACCCATGT TCTCCTGGCC ACGACTGAGA 
GATGCAGATC CTGTACTTCG CTGTGAGATG GCTTCCACTG GAGAAGTAGC TTGTTTTGGA 
CCAAACATTT ACTCGGCCTT CCTGAAAGCT ATGCTGTCCA CAGGCTTTAA GTTGCCTCAG 
AAGGGCATCC TGATTGGGAT TCAGCATTCG TTCCGACCCA GTTTCTTGGC TACAGCAAAC 
CAGCTGAAAG AGGAAGGCTT CAAGCTCTAT GCTACTGAAG CCACTTCCGC TTGGTTGTGT 
GCCAATGACG TTCCTGCCAT TCCAGTAGCC TGGCCCACTG GGATGGGTGG AGACAACAGC 
TTGCCCAGTA TTAAGAGATT GATCAGTGAG CGTCATATCG ACCTGGTGGT CAACTTGCCC 
AATAACAATA CTCGACACGT GAAGGATAAC TTCCTCATCC GCAGAATGGC CATTGACCAT 
GGAGTCCCAC TCATTACCAA TTTTCAGGTG GTAAAGCTAT TTGCTGAGGC GATCCACTAT 
GCAGCTCAGC TGGACACTAC AAGTCTTTTC CACTACCGAC AGAAAGAAAG CCAACAAAGA 
GGGTCTGGCC AGCAGGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cps1 cDNA ORF clone, Maylandia zebra(zebra mbuna)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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