LOC101477218 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101477218
Entrez Gene ID	101477218
Full Name	rho guanine nucleotide exchange factor 40
Synonyms	arhgef40
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004563576.2	XP_004563633.1	rho guanine nucleotide exchange factor 40 isoform X1
XM_023153878.2	XP_023009646.2	pleckstrin homology domain-containing family G member 4B isoform X2
XM_004563576.1	XP_004563633.1	rho guanine nucleotide exchange factor 40
XM_004563576.3	XP_004563633.2	pleckstrin homology domain-containing family G member 4B isoform X1
XM_023153878.1	XP_023009646.1	rho guanine nucleotide exchange factor 40 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101477218 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101477218 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa13582	XM_004563576.2 Latest version!	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa310617	XM_023153878.1 Latest version!	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa370661	XM_004563576.3 Latest version!	Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa370663	XM_023153878.2 Latest version!	Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMa13582	XM_004563576.1	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101477218 ( XM_004563576.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004563633.1 Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X1, mRNA.

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CloneID	OMa13582
Clone ID Related Accession (Same CDS sequence)	XM_004563576.2 , XM_004563576.1
Accession Version	XM_004563576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 40 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445762.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004563576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTACT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAAACTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTGGTGGT AGTAGTGGCG GAGGAGTGTT GATCCGAGGA 
GTGGAGGTCA GCAGCAAGGA GGTGGTGGAT CACACGGGGT CACCGCGCCA GCATGTACTG 
CTGGGGAGGA CAGACAGGGA GACGGGGACG GATAGGGCCG GTTCAACCGC ACAGAGTAAG 
CTGTACCTGC TCTGGTGTAG GATGCTAAGT TCAGAGAGGC AGTACGTGAC TGTGCTAAAG 
GGGGTAGAAG AGACGTACCT GCCCTTACTG GAGCTCTCAG ACACCCCAGC TTCCATCAGG 
GGGAAGGCAG ACTCGCTGTT TCCCAACTGG GCCAGCCTCA CCACCTTTCA CTCGCAGGAC 
CTACTCCCAG CCATGGAGGG CGCTCTTGTG CAAAGCCTGT TGCAACAGGA CTGCTTCAGT 
AAATTTCGGG ACCAGTTTCT GCAGTATTCC CAGTACATCA GGACCAAACC AGACCTGGAT 
TCTCCACTGG TCACACAAGC AGCCGACTTT TTCAAGTCAA AACTTCCCCA AGCCTCCCCC 
GTCTCCCCTC TGGCCTTCCC TCACTGTCTG CAGGCTCCCA TTCAGAGGCT GGAGCAGTAC 
TGCGAGGCGC TGGAGGAGCT GGGAGGCATA AACCCCGCCT CAGACTCCGC CCTCTCCATC 
CTGAGACACG CCCAGCGGCA CGGCGAGGAC CTCAGGGCCA GTGACCTCAT CGTCGGATGC 
CCGATCCCTG TGACGGAGCG CGGCGAGCTG GTGCGGCAGG GTGAGCTGAC GGTGAGCGGG 
GGAACTCGGA GGAAGCGGGC GGGCGTGAGG AACGTTTTCC TTTATCAACA CGTCGTAATC 
TTCACTAAAC ACAAGAATCC CAGCCCGGGA CGCACCGCCT ACAGTTACAA ACACAGCATC 
AAGACGAATG AGATGGGCCT GACTCAGAGC GTCGGCGAGG AGGGAGTGAA GTTCGAGGTG 
TGGGTCCGAC AGGCCCCGAG GACCAGAGAC TGCATCACCC TGCAAGCGCG CGACAGGGAA 
GACCGAGAGG CCTGGACTCA CGACATAGCC CATCTGCTGT GGACACATGC CATCAACAAT 
ACAGAGTTGT GTTTGAAGGA GTCACTGTGC ATGGGAGTTT CCAGTAGGCT TCTGTTGGAT 
GCAACAGGAA CTCCAGGCTC AGAGTTGGAC TCCATCTACA GTCTCAGCGA CAGAATCCAC 
AGTAGCTGTT CAGACACTTC TTCTGTCGGG AGTCAGAAGG AGGGGGGATC TCCAGCGTCT 
GGGAGGGACC CCAGGAGAAG CTCTGGATCA ACAAGCTGCT CACAGAGCCA GTCTCCCTCT 
ACAGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004563633.1
CDS	177..5408
Translation	MCVYLCGSEAMEDCVQGALSSLYPPFESTAPPLLSQVFSVLESTYQHDSLRYLLDYFVPAKHLLHKLQQHACSQYLGCLFLHSGWPLCLGEKVVVQLSTLDWRLLRSNDFYLQVVPFSTRCPRLALKCLAPGGRTVQEILVPESQHPLVFTTEWLHIINKERGHKREVGGGLDTCLVSTCDGVVRLPWKEVVYPKFIHDPSEELGLMSNRLPSEGGSSLGGWGGSSSGELDSWSWDEEEDEDLQPDGLDSDCVLPRRRRSEDGLGRTSRQPQLDGDYVELLEPRGGPDGGVDPRQRYQEMHGICKTKTLPLCRRGKAIKVRRGKAWGYGRTERSGSFRAILGIKGDAVAAKGEALPPLPVPGVAEPGRGRRSYSSSVHDSDEGDKTNRDSEGLESRRLCFDGPFKERRPGVGKERDSNGHTQLCKDDHKSKESKSNNSLDTGEIHELTNHKDGHVVEGHSDHGSHSDSVFEDADKPLSGDSEANTPTSDAPQRPFAAVSKSGEITNTGSKKKSLSNQKSMDEKDEGKVEDRVCPRGKEVKTAGFRAPRRKRKGKGAKGKAKTGGRVKEKALKMQVKTPQPSPATCTASTKLPTAKESQTEEAEQADQTEGASAADSEATDNTVKSSAEAESSLPVCNGQSGTSFFNHSNEEAASPDKQNGDASKEPALLRELDADLLQSGKLKLTGTVDRLGRVLVFTEADASEVGFSGEEMARVLACYHTITRPAAKEKGLTVLMDSRRAPPSALLFSALKLFQGSVPGGLGSLLVLVEEQQESPSHSLEGAEVHTVRGTSVLQQYVDKQQLPKEVDGDFIHCHADWLAFRLSLESLTERCESALSLLGEALQSMDAEPMPDNIKAVPLSIDKHRQLMASVLADQRLTELQQKGGAWLAGLTNRASGLAQKSPDCRAALAVTSKLYDSVDDALHRLVRVSNQRGRDLEALGRLAGLVDKLEKCDKEIEQVQSQLEDYKDPPLSLSRLSLKQQRFKTFKETANELHSETLSLLSDLEGWCELDWAGLSNVQIRLPPVREKLRDMSHCLSDCWTTLDNTQRLLSTLTEATQWCDAVSSTSSSSSSSTCPLASLPPIPPSRFQDARSLAIELGGGALLDLWTQTVERYQRTVAQVKPRFLQSDRTQNQGQGKTKTPSASSLWDLMGPEGEGDSWGLGAGGGEGGLQSWGSLASLFRPQTCSTLKIGEEKGNKKEGTAGGSGAGGGGAGGGKFLSNLLNPTKKSPTEAPLPFKPPRKRHPSFDLQALLAPRRGTATPKPAESPVAGASRNSPMSWLGRRALADQVITTSMAAAIPGWSGGGGGSSGGGVLIRGVEVSSKEVVDHTGSPRQHVLLGRTDRETGTDRAGSTAQSKLYLLWCRMLSSERQYVTVLKGVEETYLPLLELSDTPASIRGKADSLFPNWASLTTFHSQDLLPAMEGALVQSLLQQDCFSKFRDQFLQYSQYIRTKPDLDSPLVTQAADFFKSKLPQASPVSPLAFPHCLQAPIQRLEQYCEALEELGGINPASDSALSILRHAQRHGEDLRASDLIVGCPIPVTERGELVRQGELTVSGGTRRKRAGVRNVFLYQHVVIFTKHKNPSPGRTAYSYKHSIKTNEMGLTQSVGEEGVKFEVWVRQAPRTRDCITLQARDREDREAWTHDIAHLLWTHAINNTELCLKESLCMGVSSRLLLDATGTPGSELDSIYSLSDRIHSSCSDTSSVGSQKEGGSPASGRDPRRSSGSTSCSQSQSPSTAV

Target ORF information:

RefSeq Version	XM_004563576.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004563576.2

ORF Insert Sequence:

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTACT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAAACTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTGGTGGT AGTAGTGGCG GAGGAGTGTT GATCCGAGGA 
GTGGAGGTCA GCAGCAAGGA GGTGGTGGAT CACACGGGGT CACCGCGCCA GCATGTACTG 
CTGGGGAGGA CAGACAGGGA GACGGGGACG GATAGGGCCG GTTCAACCGC ACAGAGTAAG 
CTGTACCTGC TCTGGTGTAG GATGCTAAGT TCAGAGAGGC AGTACGTGAC TGTGCTAAAG 
GGGGTAGAAG AGACGTACCT GCCCTTACTG GAGCTCTCAG ACACCCCAGC TTCCATCAGG 
GGGAAGGCAG ACTCGCTGTT TCCCAACTGG GCCAGCCTCA CCACCTTTCA CTCGCAGGAC 
CTACTCCCAG CCATGGAGGG CGCTCTTGTG CAAAGCCTGT TGCAACAGGA CTGCTTCAGT 
AAATTTCGGG ACCAGTTTCT GCAGTATTCC CAGTACATCA GGACCAAACC AGACCTGGAT 
TCTCCACTGG TCACACAAGC AGCCGACTTT TTCAAGTCAA AACTTCCCCA AGCCTCCCCC 
GTCTCCCCTC TGGCCTTCCC TCACTGTCTG CAGGCTCCCA TTCAGAGGCT GGAGCAGTAC 
TGCGAGGCGC TGGAGGAGCT GGGAGGCATA AACCCCGCCT CAGACTCCGC CCTCTCCATC 
CTGAGACACG CCCAGCGGCA CGGCGAGGAC CTCAGGGCCA GTGACCTCAT CGTCGGATGC 
CCGATCCCTG TGACGGAGCG CGGCGAGCTG GTGCGGCAGG GTGAGCTGAC GGTGAGCGGG 
GGAACTCGGA GGAAGCGGGC GGGCGTGAGG AACGTTTTCC TTTATCAACA CGTCGTAATC 
TTCACTAAAC ACAAGAATCC CAGCCCGGGA CGCACCGCCT ACAGTTACAA ACACAGCATC 
AAGACGAATG AGATGGGCCT GACTCAGAGC GTCGGCGAGG AGGGAGTGAA GTTCGAGGTG 
TGGGTCCGAC AGGCCCCGAG GACCAGAGAC TGCATCACCC TGCAAGCGCG CGACAGGGAA 
GACCGAGAGG CCTGGACTCA CGACATAGCC CATCTGCTGT GGACACATGC CATCAACAAT 
ACAGAGTTGT GTTTGAAGGA GTCACTGTGC ATGGGAGTTT CCAGTAGGCT TCTGTTGGAT 
GCAACAGGAA CTCCAGGCTC AGAGTTGGAC TCCATCTACA GTCTCAGCGA CAGAATCCAC 
AGTAGCTGTT CAGACACTTC TTCTGTCGGG AGTCAGAAGG AGGGGGGATC TCCAGCGTCT 
GGGAGGGACC CCAGGAGAAG CTCTGGATCA ACAAGCTGCT CACAGAGCCA GTCTCCCTCT 
ACAGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477218 ( XM_004563576.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004563633.1 Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), mRNA.

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CloneID	OMa13582
Clone ID Related Accession (Same CDS sequence)	XM_004563576.2 , XM_004563576.1
Accession Version	XM_004563576.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	rho guanine nucleotide exchange factor 40
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445762.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTACT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAAACTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTGGTGGT AGTAGTGGCG GAGGAGTGTT GATCCGAGGA 
GTGGAGGTCA GCAGCAAGGA GGTGGTGGAT CACACGGGGT CACCGCGCCA GCATGTACTG 
CTGGGGAGGA CAGACAGGGA GACGGGGACG GATAGGGCCG GTTCAACCGC ACAGAGTAAG 
CTGTACCTGC TCTGGTGTAG GATGCTAAGT TCAGAGAGGC AGTACGTGAC TGTGCTAAAG 
GGGGTAGAAG AGACGTACCT GCCCTTACTG GAGCTCTCAG ACACCCCAGC TTCCATCAGG 
GGGAAGGCAG ACTCGCTGTT TCCCAACTGG GCCAGCCTCA CCACCTTTCA CTCGCAGGAC 
CTACTCCCAG CCATGGAGGG CGCTCTTGTG CAAAGCCTGT TGCAACAGGA CTGCTTCAGT 
AAATTTCGGG ACCAGTTTCT GCAGTATTCC CAGTACATCA GGACCAAACC AGACCTGGAT 
TCTCCACTGG TCACACAAGC AGCCGACTTT TTCAAGTCAA AACTTCCCCA AGCCTCCCCC 
GTCTCCCCTC TGGCCTTCCC TCACTGTCTG CAGGCTCCCA TTCAGAGGCT GGAGCAGTAC 
TGCGAGGCGC TGGAGGAGCT GGGAGGCATA AACCCCGCCT CAGACTCCGC CCTCTCCATC 
CTGAGACACG CCCAGCGGCA CGGCGAGGAC CTCAGGGCCA GTGACCTCAT CGTCGGATGC 
CCGATCCCTG TGACGGAGCG CGGCGAGCTG GTGCGGCAGG GTGAGCTGAC GGTGAGCGGG 
GGAACTCGGA GGAAGCGGGC GGGCGTGAGG AACGTTTTCC TTTATCAACA CGTCGTAATC 
TTCACTAAAC ACAAGAATCC CAGCCCGGGA CGCACCGCCT ACAGTTACAA ACACAGCATC 
AAGACGAATG AGATGGGCCT GACTCAGAGC GTCGGCGAGG AGGGAGTGAA GTTCGAGGTG 
TGGGTCCGAC AGGCCCCGAG GACCAGAGAC TGCATCACCC TGCAAGCGCG CGACAGGGAA 
GACCGAGAGG CCTGGACTCA CGACATAGCC CATCTGCTGT GGACACATGC CATCAACAAT 
ACAGAGTTGT GTTTGAAGGA GTCACTGTGC ATGGGAGTTT CCAGTAGGCT TCTGTTGGAT 
GCAACAGGAA CTCCAGGCTC AGAGTTGGAC TCCATCTACA GTCTCAGCGA CAGAATCCAC 
AGTAGCTGTT CAGACACTTC TTCTGTCGGG AGTCAGAAGG AGGGGGGATC TCCAGCGTCT 
GGGAGGGACC CCAGGAGAAG CTCTGGATCA ACAAGCTGCT CACAGAGCCA GTCTCCCTCT 
ACAGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004563633.1
CDS	173..5404
Translation	MCVYLCGSEAMEDCVQGALSSLYPPFESTAPPLLSQVFSVLESTYQHDSLRYLLDYFVPAKHLLHKLQQHACSQYLGCLFLHSGWPLCLGEKVVVQLSTLDWRLLRSNDFYLQVVPFSTRCPRLALKCLAPGGRTVQEILVPESQHPLVFTTEWLHIINKERGHKREVGGGLDTCLVSTCDGVVRLPWKEVVYPKFIHDPSEELGLMSNRLPSEGGSSLGGWGGSSSGELDSWSWDEEEDEDLQPDGLDSDCVLPRRRRSEDGLGRTSRQPQLDGDYVELLEPRGGPDGGVDPRQRYQEMHGICKTKTLPLCRRGKAIKVRRGKAWGYGRTERSGSFRAILGIKGDAVAAKGEALPPLPVPGVAEPGRGRRSYSSSVHDSDEGDKTNRDSEGLESRRLCFDGPFKERRPGVGKERDSNGHTQLCKDDHKSKESKSNNSLDTGEIHELTNHKDGHVVEGHSDHGSHSDSVFEDADKPLSGDSEANTPTSDAPQRPFAAVSKSGEITNTGSKKKSLSNQKSMDEKDEGKVEDRVCPRGKEVKTAGFRAPRRKRKGKGAKGKAKTGGRVKEKALKMQVKTPQPSPATCTASTKLPTAKESQTEEAEQADQTEGASAADSEATDNTVKSSAEAESSLPVCNGQSGTSFFNHSNEEAASPDKQNGDASKEPALLRELDADLLQSGKLKLTGTVDRLGRVLVFTEADASEVGFSGEEMARVLACYHTITRPAAKEKGLTVLMDSRRAPPSALLFSALKLFQGSVPGGLGSLLVLVEEQQESPSHSLEGAEVHTVRGTSVLQQYVDKQQLPKEVDGDFIHCHADWLAFRLSLESLTERCESALSLLGEALQSMDAEPMPDNIKAVPLSIDKHRQLMASVLADQRLTELQQKGGAWLAGLTNRASGLAQKSPDCRAALAVTSKLYDSVDDALHRLVRVSNQRGRDLEALGRLAGLVDKLEKCDKEIEQVQSQLEDYKDPPLSLSRLSLKQQRFKTFKETANELHSETLSLLSDLEGWCELDWAGLSNVQIRLPPVREKLRDMSHCLSDCWTTLDNTQRLLSTLTEATQWCDAVSSTSSSSSSSTCPLASLPPIPPSRFQDARSLAIELGGGALLDLWTQTVERYQRTVAQVKPRFLQSDRTQNQGQGKTKTPSASSLWDLMGPEGEGDSWGLGAGGGEGGLQSWGSLASLFRPQTCSTLKIGEEKGNKKEGTAGGSGAGGGGAGGGKFLSNLLNPTKKSPTEAPLPFKPPRKRHPSFDLQALLAPRRGTATPKPAESPVAGASRNSPMSWLGRRALADQVITTSMAAAIPGWSGGGGGSSGGGVLIRGVEVSSKEVVDHTGSPRQHVLLGRTDRETGTDRAGSTAQSKLYLLWCRMLSSERQYVTVLKGVEETYLPLLELSDTPASIRGKADSLFPNWASLTTFHSQDLLPAMEGALVQSLLQQDCFSKFRDQFLQYSQYIRTKPDLDSPLVTQAADFFKSKLPQASPVSPLAFPHCLQAPIQRLEQYCEALEELGGINPASDSALSILRHAQRHGEDLRASDLIVGCPIPVTERGELVRQGELTVSGGTRRKRAGVRNVFLYQHVVIFTKHKNPSPGRTAYSYKHSIKTNEMGLTQSVGEEGVKFEVWVRQAPRTRDCITLQARDREDREAWTHDIAHLLWTHAINNTELCLKESLCMGVSSRLLLDATGTPGSELDSIYSLSDRIHSSCSDTSSVGSQKEGGSPASGRDPRRSSGSTSCSQSQSPSTAV

Target ORF information:

RefSeq Version	XM_004563576.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004563576.1

ORF Insert Sequence:

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTACT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAAACTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTGGTGGT AGTAGTGGCG GAGGAGTGTT GATCCGAGGA 
GTGGAGGTCA GCAGCAAGGA GGTGGTGGAT CACACGGGGT CACCGCGCCA GCATGTACTG 
CTGGGGAGGA CAGACAGGGA GACGGGGACG GATAGGGCCG GTTCAACCGC ACAGAGTAAG 
CTGTACCTGC TCTGGTGTAG GATGCTAAGT TCAGAGAGGC AGTACGTGAC TGTGCTAAAG 
GGGGTAGAAG AGACGTACCT GCCCTTACTG GAGCTCTCAG ACACCCCAGC TTCCATCAGG 
GGGAAGGCAG ACTCGCTGTT TCCCAACTGG GCCAGCCTCA CCACCTTTCA CTCGCAGGAC 
CTACTCCCAG CCATGGAGGG CGCTCTTGTG CAAAGCCTGT TGCAACAGGA CTGCTTCAGT 
AAATTTCGGG ACCAGTTTCT GCAGTATTCC CAGTACATCA GGACCAAACC AGACCTGGAT 
TCTCCACTGG TCACACAAGC AGCCGACTTT TTCAAGTCAA AACTTCCCCA AGCCTCCCCC 
GTCTCCCCTC TGGCCTTCCC TCACTGTCTG CAGGCTCCCA TTCAGAGGCT GGAGCAGTAC 
TGCGAGGCGC TGGAGGAGCT GGGAGGCATA AACCCCGCCT CAGACTCCGC CCTCTCCATC 
CTGAGACACG CCCAGCGGCA CGGCGAGGAC CTCAGGGCCA GTGACCTCAT CGTCGGATGC 
CCGATCCCTG TGACGGAGCG CGGCGAGCTG GTGCGGCAGG GTGAGCTGAC GGTGAGCGGG 
GGAACTCGGA GGAAGCGGGC GGGCGTGAGG AACGTTTTCC TTTATCAACA CGTCGTAATC 
TTCACTAAAC ACAAGAATCC CAGCCCGGGA CGCACCGCCT ACAGTTACAA ACACAGCATC 
AAGACGAATG AGATGGGCCT GACTCAGAGC GTCGGCGAGG AGGGAGTGAA GTTCGAGGTG 
TGGGTCCGAC AGGCCCCGAG GACCAGAGAC TGCATCACCC TGCAAGCGCG CGACAGGGAA 
GACCGAGAGG CCTGGACTCA CGACATAGCC CATCTGCTGT GGACACATGC CATCAACAAT 
ACAGAGTTGT GTTTGAAGGA GTCACTGTGC ATGGGAGTTT CCAGTAGGCT TCTGTTGGAT 
GCAACAGGAA CTCCAGGCTC AGAGTTGGAC TCCATCTACA GTCTCAGCGA CAGAATCCAC 
AGTAGCTGTT CAGACACTTC TTCTGTCGGG AGTCAGAAGG AGGGGGGATC TCCAGCGTCT 
GGGAGGGACC CCAGGAGAAG CTCTGGATCA ACAAGCTGCT CACAGAGCCA GTCTCCCTCT 
ACAGCTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477218 ( XM_023153878.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009646.1 Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa310617
Clone ID Related Accession (Same CDS sequence)	XM_023153878.1
Accession Version	XM_023153878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	rho guanine nucleotide exchange factor 40 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445762.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCAG AGGCAATGGA GGACTGCGTG CAGGGGGCGC TCTCATCGCT TTATCCTCCT 
TTTGAGAGCA CAGCCCCACC CCTCCTCTCC CAGGTCTTCT CAGTCCTGGA GTCGACCTAT 
CAACACGACA GTCTTCGCTA TCTCCTGGAC TACTTCGTAC CTGCAAAGCA CCTCCTGCAC 
AAACTCCAGC AGCATGCCTG TTCTCAGTAC CTGGGTTGCT TGTTCCTTCA CTCTGGCTGG 
CCTCTCTGCC TGGGCGAAAA AGTGGTTGTC CAACTTTCCA CTTTGGACTG GAGGCTTCTG 
CGCAGTAATG ACTTCTACCT ACAGGTGGTT CCCTTCTCTA CACGTTGCCC AAGGCTGGCA 
CTAAAATGCC TGGCTCCTGG GGGACGGACT GTTCAGGAAA TCTTGGTGCC CGAGTCACAG 
CATCCCCTTG TGTTCACCAC TGAGTGGCTT CACATCATCA ACAAAGAGCG AGGTCACAAG 
AGAGAAGTTG GAGGAGGACT TGACACCTGT CTGGTCAGTA CATGCGATGG TGTGGTTAGA 
CTTCCATGGA AGGAGGTAGT CTACCCAAAA TTTATCCACG ACCCCTCTGA AGAGTTGGGC 
CTAATGTCCA ACCGTCTGCC TAGCGAGGGG GGTAGCAGCC TCGGAGGCTG GGGTGGCTCA 
TCCTCTGGGG AATTAGACTC CTGGTCCTGG GATGAAGAAG AAGATGAGGA TTTGCAACCG 
GATGGCTTGG ACTCTGACTG TGTGCTTCCT CGACGAAGGC GCAGTGAAGA CGGCCTTGGG 
CGGACATCGA GACAACCTCA GCTCGATGGG GATTATGTCG AGCTTTTGGA ACCCAGGGGG 
GGTCCTGATG GAGGAGTCGA TCCAAGGCAG CGGTACCAAG AGATGCATGG AATCTGTAAG 
ACCAAGACTC TACCTCTTTG CAGGAGAGGA AAGGCTATCA AGGTTCGCAG AGGAAAAGCA 
TGGGGATATG GGCGAACGGA AAGGTCAGGA AGCTTTCGGG CTATTCTTGG CATCAAAGGA 
GACGCTGTGG CTGCCAAGGG TGAAGCATTA CCGCCACTGC CTGTGCCAGG AGTTGCCGAG 
CCTGGCCGAG GGAGGAGGTC TTACTCCTCC TCAGTCCATG ACTCTGATGA AGGAGATAAA 
ACAAACAGAG ACTCTGAAGG CCTGGAAAGC CGCCGCCTTT GTTTTGATGG CCCATTCAAG 
GAAAGGAGAC CTGGAGTGGG CAAGGAGAGA GATAGCAATG GTCACACACA GCTATGTAAA 
GATGACCACA AAAGTAAAGA ATCCAAGAGC AACAACAGCT TGGACACAGG TGAAATCCAT 
GAGTTAACAA ACCACAAAGA TGGACACGTT GTAGAGGGCC ACTCTGATCA CGGATCACAT 
TCGGACTCTG TTTTTGAGGA TGCTGATAAA CCACTGAGCG GAGACAGTGA GGCTAATACC 
CCAACATCAG ATGCACCACA GAGACCATTC GCTGCTGTTT CAAAAAGTGG TGAAATTACA 
AACACTGGGT CTAAAAAGAA GAGTTTGTCA AACCAAAAAT CCATGGATGA AAAGGATGAA 
GGAAAAGTGG AGGACAGAGT ATGCCCCAGA GGAAAAGAGG TTAAAACGGC AGGATTCAGA 
GCACCAAGGA GGAAAAGGAA GGGAAAGGGG GCCAAAGGGA AAGCTAAAAC TGGGGGTCGT 
GTAAAGGAGA AGGCCTTGAA AATGCAAGTT AAAACTCCTC AACCAAGCCC AGCCACCTGC 
ACTGCTTCAA CAAAACTGCC GACTGCCAAG GAGAGCCAAA CAGAGGAAGC AGAGCAGGCT 
GACCAAACTG AAGGTGCTTC TGCTGCAGAT AGCGAGGCGA CAGACAACAC AGTGAAAAGC 
AGCGCAGAAG CTGAATCATC TCTGCCTGTG TGTAACGGAC AGTCGGGCAC ATCTTTTTTT 
AACCACTCTA ATGAGGAGGC TGCGTCACCT GACAAACAGA ATGGTGATGC TTCAAAAGAG 
CCCGCCCTGC TGAGGGAACT GGACGCAGAC CTTCTCCAGT CAGGGAAGCT CAAGTTGACA 
GGTACAGTGG ACAGGCTGGG GCGTGTTCTG GTTTTCACAG AGGCCGACGC ATCAGAAGTG 
GGTTTCTCTG GGGAGGAGAT GGCCCGCGTC TTGGCCTGTT ACCACACAAT CACGCGGCCT 
GCAGCCAAAG AAAAGGGTTT AACAGTGTTA ATGGACAGCA GGCGCGCCCC ACCCTCCGCT 
CTCTTGTTCT CTGCTCTTAA ATTGTTCCAG GGGTCGGTCC CCGGTGGTCT TGGATCCCTT 
CTGGTGTTGG TGGAAGAGCA GCAGGAGTCC CCTTCTCATT CCCTGGAAGG AGCAGAGGTC 
CACACAGTGC GAGGAACGAG TGTCCTTCAG CAGTATGTAG ACAAACAACA GCTGCCCAAA 
GAAGTAGACG GGGACTTTAT CCACTGCCAC GCGGACTGGC TGGCCTTCAG ACTGAGTCTG 
GAGAGTCTGA CAGAGCGCTG TGAGAGCGCC CTCTCACTGC TTGGAGAAGC ACTGCAGTCT 
ATGGACGCTG AGCCAATGCC AGACAACATA AAGGCTGTTC CTCTGAGCAT TGACAAACAC 
AGGCAGCTCA TGGCCAGCGT CCTGGCCGAC CAACGGTTAA CTGAGCTGCA GCAGAAGGGC 
GGGGCCTGGC TTGCTGGATT GACAAATAGA GCATCTGGAC TGGCACAAAA GTCACCAGAC 
TGCAGAGCTG CTCTTGCCGT AACTTCTAAA CTTTATGACA GCGTGGACGA TGCACTCCAT 
CGGCTTGTCC GAGTGTCCAA CCAAAGAGGT CGTGACCTGG AGGCGTTGGG GCGTCTGGCG 
GGGTTGGTGG ACAAACTGGA AAAGTGCGAT AAAGAGATTG AGCAGGTTCA GTCGCAGCTG 
GAGGACTACA AGGATCCTCC TCTCTCCCTC AGCAGACTCT CGCTCAAACA GCAGAGGTTC 
AAGACGTTCA AAGAAACGGC CAATGAGCTG CACAGTGAGA CTCTATCACT GCTCAGTGAT 
TTGGAGGGCT GGTGTGAACT GGACTGGGCA GGCCTGAGCA ACGTCCAGAT CCGACTCCCT 
CCGGTCAGGG AGAAGCTCAG GGACATGTCC CACTGTCTGT CTGACTGCTG GACCACTCTG 
GACAACACGC AGAGGTTGTT GTCCACTCTG ACCGAGGCCA CCCAGTGGTG CGATGCAGTC 
TCCTCCACCT CCTCCTCTTC CTCTTCTTCC ACCTGCCCTC TCGCCTCCCT CCCTCCAATA 
CCCCCGTCCC GTTTCCAGGA CGCACGCTCT TTAGCAATAG AGCTGGGTGG CGGGGCACTG 
CTGGATCTCT GGACTCAGAC AGTTGAGCGA TACCAGCGAA CAGTAGCACA GGTCAAGCCC 
CGATTCCTCC AGTCTGACCG GACGCAAAAT CAGGGACAGG GGAAAACCAA AACTCCCTCT 
GCCAGCAGCC TCTGGGACCT GATGGGCCCC GAGGGAGAGG GTGACTCCTG GGGGCTGGGA 
GCTGGCGGAG GGGAAGGCGG ATTGCAGTCC TGGGGATCCC TGGCATCACT GTTCAGACCG 
CAGACCTGCT CCACACTGAA GATAGGAGAG GAGAAAGGGA ATAAGAAAGA GGGAACGGCA 
GGAGGAAGTG GTGCGGGAGG AGGTGGGGCC GGGGGAGGGA AGTTTCTATC GAACCTTCTG 
AATCCTACAA AGAAGAGCCC CACAGAGGCT CCACTTCCCT TCAAGCCACC TCGGAAGCGT 
CACCCAAGCT TTGACCTCCA GGCTCTCCTG GCCCCCCGTC GAGGTACAGC CACCCCCAAA 
CCCGCTGAGT CGCCGGTGGC TGGGGCGAGC CGTAACTCCC CCATGTCCTG GCTGGGACGA 
CGGGCTTTGG CCGACCAAGT CATTACTACT AGCATGGCTG CAGCTATTCC AGGATGGAGC 
GGTGGTGGTG GTGGTAGTAG TGGCGGAGGA GTGTTGATCC GAGGAGTGGA GGTCAGCAGC 
AAGGAGGTGG TGGATCACAC GGGGTCACCG CGCCAGCATG TACTGCTGGG GAGGACAGAC 
AGGGAGACGG GGACGGATAG GGCCGGTTCA ACCGCACAGA GTAAGCTGTA CCTGCTCTGG 
TGTAGGATGC TAAGTTCAGA GAGGCAGTAC GTGACTGTGC TAAAGGGGGT AGAAGAGACG 
TACCTGCCCT TACTGGAGCT CTCAGACACC CCAGCTTCCA TCAGGGGGAA GGCAGACTCG 
CTGTTTCCCA ACTGGGCCAG CCTCACCACC TTTCACTCGC AGGACCTACT CCCAGCCATG 
GAGGGCGCTC TTGTGCAAAG CCTGTTGCAA CAGGACTGCT TCAGTAAATT TCGGGACCAG 
TTTCTGCAGT ATTCCCAGTA CATCAGGACC AAACCAGACC TGGATTCTCC ACTGGTCACA 
CAAGCAGCCG ACTTTTTCAA GTCAAAACTT CCCCAAGCCT CCCCCGTCTC CCCTCTGGCC 
TTCCCTCACT GTCTGCAGGC TCCCATTCAG AGGCTGGAGC AGTACTGCGA GGCGCTGGAG 
GAGCTGGGAG GCATAAACCC CGCCTCAGAC TCCGCCCTCT CCATCCTGAG ACACGCCCAG 
CGGCACGGCG AGGACCTCAG GGCCAGTGAC CTCATCGTCG GATGCCCGAT CCCTGTGACG 
GAGCGCGGCG AGCTGGTGCG GCAGGGTGAG CTGACGGTGA GCGGGGGAAC TCGGAGGAAG 
CGGGCGGGCG TGAGGAACGT TTTCCTTTAT CAACACGTCG TAATCTTCAC TAAACACAAG 
AATCCCAGCC CGGGACGCAC CGCCTACAGT TACAAACACA GCATCAAGAC GAATGAGATG 
GGCCTGACTC AGAGCGTCGG CGAGGAGGGA GTGAAGTTCG AGGTGTGGGT CCGACAGGCC 
CCGAGGACCA GAGACTGCAT CACCCTGCAA GCGCGCGACA GGGAAGACCG AGAGGCCTGG 
ACTCACGACA TAGCCCATCT GCTGTGGACA CATGCCATCA ACAATACAGA GTTGTGTTTG 
AAGGAGTCAC TGTGCATGGG AGTTTCCAGT AGGCTTCTGT TGGATGCAAC AGGAACTCCA 
GGCTCAGAGT TGGACTCCAT CTACAGTCTC AGCGACAGAA TCCACAGTAG CTGTTCAGAC 
ACTTCTTCTG TCGGGAGTCA GAAGGAGGGG GGATCTCCAG CGTCTGGGAG GGACCCCAGG 
AGAAGCTCTG GATCAACAAG CTGCTCACAG AGCCAGTCTC CCTCTACAGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009646.1
CDS	247..5463
Translation	MGSEAMEDCVQGALSSLYPPFESTAPPLLSQVFSVLESTYQHDSLRYLLDYFVPAKHLLHKLQQHACSQYLGCLFLHSGWPLCLGEKVVVQLSTLDWRLLRSNDFYLQVVPFSTRCPRLALKCLAPGGRTVQEILVPESQHPLVFTTEWLHIINKERGHKREVGGGLDTCLVSTCDGVVRLPWKEVVYPKFIHDPSEELGLMSNRLPSEGGSSLGGWGGSSSGELDSWSWDEEEDEDLQPDGLDSDCVLPRRRRSEDGLGRTSRQPQLDGDYVELLEPRGGPDGGVDPRQRYQEMHGICKTKTLPLCRRGKAIKVRRGKAWGYGRTERSGSFRAILGIKGDAVAAKGEALPPLPVPGVAEPGRGRRSYSSSVHDSDEGDKTNRDSEGLESRRLCFDGPFKERRPGVGKERDSNGHTQLCKDDHKSKESKSNNSLDTGEIHELTNHKDGHVVEGHSDHGSHSDSVFEDADKPLSGDSEANTPTSDAPQRPFAAVSKSGEITNTGSKKKSLSNQKSMDEKDEGKVEDRVCPRGKEVKTAGFRAPRRKRKGKGAKGKAKTGGRVKEKALKMQVKTPQPSPATCTASTKLPTAKESQTEEAEQADQTEGASAADSEATDNTVKSSAEAESSLPVCNGQSGTSFFNHSNEEAASPDKQNGDASKEPALLRELDADLLQSGKLKLTGTVDRLGRVLVFTEADASEVGFSGEEMARVLACYHTITRPAAKEKGLTVLMDSRRAPPSALLFSALKLFQGSVPGGLGSLLVLVEEQQESPSHSLEGAEVHTVRGTSVLQQYVDKQQLPKEVDGDFIHCHADWLAFRLSLESLTERCESALSLLGEALQSMDAEPMPDNIKAVPLSIDKHRQLMASVLADQRLTELQQKGGAWLAGLTNRASGLAQKSPDCRAALAVTSKLYDSVDDALHRLVRVSNQRGRDLEALGRLAGLVDKLEKCDKEIEQVQSQLEDYKDPPLSLSRLSLKQQRFKTFKETANELHSETLSLLSDLEGWCELDWAGLSNVQIRLPPVREKLRDMSHCLSDCWTTLDNTQRLLSTLTEATQWCDAVSSTSSSSSSSTCPLASLPPIPPSRFQDARSLAIELGGGALLDLWTQTVERYQRTVAQVKPRFLQSDRTQNQGQGKTKTPSASSLWDLMGPEGEGDSWGLGAGGGEGGLQSWGSLASLFRPQTCSTLKIGEEKGNKKEGTAGGSGAGGGGAGGGKFLSNLLNPTKKSPTEAPLPFKPPRKRHPSFDLQALLAPRRGTATPKPAESPVAGASRNSPMSWLGRRALADQVITTSMAAAIPGWSGGGGGSSGGGVLIRGVEVSSKEVVDHTGSPRQHVLLGRTDRETGTDRAGSTAQSKLYLLWCRMLSSERQYVTVLKGVEETYLPLLELSDTPASIRGKADSLFPNWASLTTFHSQDLLPAMEGALVQSLLQQDCFSKFRDQFLQYSQYIRTKPDLDSPLVTQAADFFKSKLPQASPVSPLAFPHCLQAPIQRLEQYCEALEELGGINPASDSALSILRHAQRHGEDLRASDLIVGCPIPVTERGELVRQGELTVSGGTRRKRAGVRNVFLYQHVVIFTKHKNPSPGRTAYSYKHSIKTNEMGLTQSVGEEGVKFEVWVRQAPRTRDCITLQARDREDREAWTHDIAHLLWTHAINNTELCLKESLCMGVSSRLLLDATGTPGSELDSIYSLSDRIHSSCSDTSSVGSQKEGGSPASGRDPRRSSGSTSCSQSQSPSTAV

Target ORF information:

RefSeq Version	XM_023153878.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra rho guanine nucleotide exchange factor 40 (LOC101477218), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153878.1

ORF Insert Sequence:

ATGGGTTCAG AGGCAATGGA GGACTGCGTG CAGGGGGCGC TCTCATCGCT TTATCCTCCT 
TTTGAGAGCA CAGCCCCACC CCTCCTCTCC CAGGTCTTCT CAGTCCTGGA GTCGACCTAT 
CAACACGACA GTCTTCGCTA TCTCCTGGAC TACTTCGTAC CTGCAAAGCA CCTCCTGCAC 
AAACTCCAGC AGCATGCCTG TTCTCAGTAC CTGGGTTGCT TGTTCCTTCA CTCTGGCTGG 
CCTCTCTGCC TGGGCGAAAA AGTGGTTGTC CAACTTTCCA CTTTGGACTG GAGGCTTCTG 
CGCAGTAATG ACTTCTACCT ACAGGTGGTT CCCTTCTCTA CACGTTGCCC AAGGCTGGCA 
CTAAAATGCC TGGCTCCTGG GGGACGGACT GTTCAGGAAA TCTTGGTGCC CGAGTCACAG 
CATCCCCTTG TGTTCACCAC TGAGTGGCTT CACATCATCA ACAAAGAGCG AGGTCACAAG 
AGAGAAGTTG GAGGAGGACT TGACACCTGT CTGGTCAGTA CATGCGATGG TGTGGTTAGA 
CTTCCATGGA AGGAGGTAGT CTACCCAAAA TTTATCCACG ACCCCTCTGA AGAGTTGGGC 
CTAATGTCCA ACCGTCTGCC TAGCGAGGGG GGTAGCAGCC TCGGAGGCTG GGGTGGCTCA 
TCCTCTGGGG AATTAGACTC CTGGTCCTGG GATGAAGAAG AAGATGAGGA TTTGCAACCG 
GATGGCTTGG ACTCTGACTG TGTGCTTCCT CGACGAAGGC GCAGTGAAGA CGGCCTTGGG 
CGGACATCGA GACAACCTCA GCTCGATGGG GATTATGTCG AGCTTTTGGA ACCCAGGGGG 
GGTCCTGATG GAGGAGTCGA TCCAAGGCAG CGGTACCAAG AGATGCATGG AATCTGTAAG 
ACCAAGACTC TACCTCTTTG CAGGAGAGGA AAGGCTATCA AGGTTCGCAG AGGAAAAGCA 
TGGGGATATG GGCGAACGGA AAGGTCAGGA AGCTTTCGGG CTATTCTTGG CATCAAAGGA 
GACGCTGTGG CTGCCAAGGG TGAAGCATTA CCGCCACTGC CTGTGCCAGG AGTTGCCGAG 
CCTGGCCGAG GGAGGAGGTC TTACTCCTCC TCAGTCCATG ACTCTGATGA AGGAGATAAA 
ACAAACAGAG ACTCTGAAGG CCTGGAAAGC CGCCGCCTTT GTTTTGATGG CCCATTCAAG 
GAAAGGAGAC CTGGAGTGGG CAAGGAGAGA GATAGCAATG GTCACACACA GCTATGTAAA 
GATGACCACA AAAGTAAAGA ATCCAAGAGC AACAACAGCT TGGACACAGG TGAAATCCAT 
GAGTTAACAA ACCACAAAGA TGGACACGTT GTAGAGGGCC ACTCTGATCA CGGATCACAT 
TCGGACTCTG TTTTTGAGGA TGCTGATAAA CCACTGAGCG GAGACAGTGA GGCTAATACC 
CCAACATCAG ATGCACCACA GAGACCATTC GCTGCTGTTT CAAAAAGTGG TGAAATTACA 
AACACTGGGT CTAAAAAGAA GAGTTTGTCA AACCAAAAAT CCATGGATGA AAAGGATGAA 
GGAAAAGTGG AGGACAGAGT ATGCCCCAGA GGAAAAGAGG TTAAAACGGC AGGATTCAGA 
GCACCAAGGA GGAAAAGGAA GGGAAAGGGG GCCAAAGGGA AAGCTAAAAC TGGGGGTCGT 
GTAAAGGAGA AGGCCTTGAA AATGCAAGTT AAAACTCCTC AACCAAGCCC AGCCACCTGC 
ACTGCTTCAA CAAAACTGCC GACTGCCAAG GAGAGCCAAA CAGAGGAAGC AGAGCAGGCT 
GACCAAACTG AAGGTGCTTC TGCTGCAGAT AGCGAGGCGA CAGACAACAC AGTGAAAAGC 
AGCGCAGAAG CTGAATCATC TCTGCCTGTG TGTAACGGAC AGTCGGGCAC ATCTTTTTTT 
AACCACTCTA ATGAGGAGGC TGCGTCACCT GACAAACAGA ATGGTGATGC TTCAAAAGAG 
CCCGCCCTGC TGAGGGAACT GGACGCAGAC CTTCTCCAGT CAGGGAAGCT CAAGTTGACA 
GGTACAGTGG ACAGGCTGGG GCGTGTTCTG GTTTTCACAG AGGCCGACGC ATCAGAAGTG 
GGTTTCTCTG GGGAGGAGAT GGCCCGCGTC TTGGCCTGTT ACCACACAAT CACGCGGCCT 
GCAGCCAAAG AAAAGGGTTT AACAGTGTTA ATGGACAGCA GGCGCGCCCC ACCCTCCGCT 
CTCTTGTTCT CTGCTCTTAA ATTGTTCCAG GGGTCGGTCC CCGGTGGTCT TGGATCCCTT 
CTGGTGTTGG TGGAAGAGCA GCAGGAGTCC CCTTCTCATT CCCTGGAAGG AGCAGAGGTC 
CACACAGTGC GAGGAACGAG TGTCCTTCAG CAGTATGTAG ACAAACAACA GCTGCCCAAA 
GAAGTAGACG GGGACTTTAT CCACTGCCAC GCGGACTGGC TGGCCTTCAG ACTGAGTCTG 
GAGAGTCTGA CAGAGCGCTG TGAGAGCGCC CTCTCACTGC TTGGAGAAGC ACTGCAGTCT 
ATGGACGCTG AGCCAATGCC AGACAACATA AAGGCTGTTC CTCTGAGCAT TGACAAACAC 
AGGCAGCTCA TGGCCAGCGT CCTGGCCGAC CAACGGTTAA CTGAGCTGCA GCAGAAGGGC 
GGGGCCTGGC TTGCTGGATT GACAAATAGA GCATCTGGAC TGGCACAAAA GTCACCAGAC 
TGCAGAGCTG CTCTTGCCGT AACTTCTAAA CTTTATGACA GCGTGGACGA TGCACTCCAT 
CGGCTTGTCC GAGTGTCCAA CCAAAGAGGT CGTGACCTGG AGGCGTTGGG GCGTCTGGCG 
GGGTTGGTGG ACAAACTGGA AAAGTGCGAT AAAGAGATTG AGCAGGTTCA GTCGCAGCTG 
GAGGACTACA AGGATCCTCC TCTCTCCCTC AGCAGACTCT CGCTCAAACA GCAGAGGTTC 
AAGACGTTCA AAGAAACGGC CAATGAGCTG CACAGTGAGA CTCTATCACT GCTCAGTGAT 
TTGGAGGGCT GGTGTGAACT GGACTGGGCA GGCCTGAGCA ACGTCCAGAT CCGACTCCCT 
CCGGTCAGGG AGAAGCTCAG GGACATGTCC CACTGTCTGT CTGACTGCTG GACCACTCTG 
GACAACACGC AGAGGTTGTT GTCCACTCTG ACCGAGGCCA CCCAGTGGTG CGATGCAGTC 
TCCTCCACCT CCTCCTCTTC CTCTTCTTCC ACCTGCCCTC TCGCCTCCCT CCCTCCAATA 
CCCCCGTCCC GTTTCCAGGA CGCACGCTCT TTAGCAATAG AGCTGGGTGG CGGGGCACTG 
CTGGATCTCT GGACTCAGAC AGTTGAGCGA TACCAGCGAA CAGTAGCACA GGTCAAGCCC 
CGATTCCTCC AGTCTGACCG GACGCAAAAT CAGGGACAGG GGAAAACCAA AACTCCCTCT 
GCCAGCAGCC TCTGGGACCT GATGGGCCCC GAGGGAGAGG GTGACTCCTG GGGGCTGGGA 
GCTGGCGGAG GGGAAGGCGG ATTGCAGTCC TGGGGATCCC TGGCATCACT GTTCAGACCG 
CAGACCTGCT CCACACTGAA GATAGGAGAG GAGAAAGGGA ATAAGAAAGA GGGAACGGCA 
GGAGGAAGTG GTGCGGGAGG AGGTGGGGCC GGGGGAGGGA AGTTTCTATC GAACCTTCTG 
AATCCTACAA AGAAGAGCCC CACAGAGGCT CCACTTCCCT TCAAGCCACC TCGGAAGCGT 
CACCCAAGCT TTGACCTCCA GGCTCTCCTG GCCCCCCGTC GAGGTACAGC CACCCCCAAA 
CCCGCTGAGT CGCCGGTGGC TGGGGCGAGC CGTAACTCCC CCATGTCCTG GCTGGGACGA 
CGGGCTTTGG CCGACCAAGT CATTACTACT AGCATGGCTG CAGCTATTCC AGGATGGAGC 
GGTGGTGGTG GTGGTAGTAG TGGCGGAGGA GTGTTGATCC GAGGAGTGGA GGTCAGCAGC 
AAGGAGGTGG TGGATCACAC GGGGTCACCG CGCCAGCATG TACTGCTGGG GAGGACAGAC 
AGGGAGACGG GGACGGATAG GGCCGGTTCA ACCGCACAGA GTAAGCTGTA CCTGCTCTGG 
TGTAGGATGC TAAGTTCAGA GAGGCAGTAC GTGACTGTGC TAAAGGGGGT AGAAGAGACG 
TACCTGCCCT TACTGGAGCT CTCAGACACC CCAGCTTCCA TCAGGGGGAA GGCAGACTCG 
CTGTTTCCCA ACTGGGCCAG CCTCACCACC TTTCACTCGC AGGACCTACT CCCAGCCATG 
GAGGGCGCTC TTGTGCAAAG CCTGTTGCAA CAGGACTGCT TCAGTAAATT TCGGGACCAG 
TTTCTGCAGT ATTCCCAGTA CATCAGGACC AAACCAGACC TGGATTCTCC ACTGGTCACA 
CAAGCAGCCG ACTTTTTCAA GTCAAAACTT CCCCAAGCCT CCCCCGTCTC CCCTCTGGCC 
TTCCCTCACT GTCTGCAGGC TCCCATTCAG AGGCTGGAGC AGTACTGCGA GGCGCTGGAG 
GAGCTGGGAG GCATAAACCC CGCCTCAGAC TCCGCCCTCT CCATCCTGAG ACACGCCCAG 
CGGCACGGCG AGGACCTCAG GGCCAGTGAC CTCATCGTCG GATGCCCGAT CCCTGTGACG 
GAGCGCGGCG AGCTGGTGCG GCAGGGTGAG CTGACGGTGA GCGGGGGAAC TCGGAGGAAG 
CGGGCGGGCG TGAGGAACGT TTTCCTTTAT CAACACGTCG TAATCTTCAC TAAACACAAG 
AATCCCAGCC CGGGACGCAC CGCCTACAGT TACAAACACA GCATCAAGAC GAATGAGATG 
GGCCTGACTC AGAGCGTCGG CGAGGAGGGA GTGAAGTTCG AGGTGTGGGT CCGACAGGCC 
CCGAGGACCA GAGACTGCAT CACCCTGCAA GCGCGCGACA GGGAAGACCG AGAGGCCTGG 
ACTCACGACA TAGCCCATCT GCTGTGGACA CATGCCATCA ACAATACAGA GTTGTGTTTG 
AAGGAGTCAC TGTGCATGGG AGTTTCCAGT AGGCTTCTGT TGGATGCAAC AGGAACTCCA 
GGCTCAGAGT TGGACTCCAT CTACAGTCTC AGCGACAGAA TCCACAGTAG CTGTTCAGAC 
ACTTCTTCTG TCGGGAGTCA GAAGGAGGGG GGATCTCCAG CGTCTGGGAG GGACCCCAGG 
AGAAGCTCTG GATCAACAAG CTGCTCACAG AGCCAGTCTC CCTCTACAGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477218 ( XM_004563576.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004563633.2 Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X1, mRNA.

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CloneID	OMa370661
Clone ID Related Accession (Same CDS sequence)	XM_004563576.3
Accession Version	XM_004563576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	pleckstrin homology domain-containing family G member 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004563576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTTCT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAATCTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTAGTAGT GGCGGAGGAG TGTTGATCCG AGGAGTGGAG 
GTCAGCAGCA AGGAGGTGGT GGATCACACG GGGTCACCGC GCCAGCATGT ACTGCTGGGG 
AGGACAGACA GGGAGACGGG GACGGATAGG GCCGGTTCAA CCGCACAGAG TAAGCTGTAC 
CTGCTCTGGT GTAGGATGCT AAGTTCAGAG AGGCAGTACG TGACTGTGCT AAAGGGGGTA 
GAAGAGACGT ACCTGCCCTT ACTGGAGCTC TCAGACACCC CAGCTTCCAT CAGGGGGAAG 
GCAGACTCGC TGTTTCCCAA CTGGGCCAGC CTCACCACCT TTCACTCGCA GGACCTACTC 
CCAGCCATGG AGGGCGCTCT TGTGCAAAGC CTGTTGCAAC AGGACTGCTT CAGTAAATTT 
CGGGACCAGT TTCTGCAGTA TTCCCAGTAC ATCAGGACCA AACCAGACCT GGATTCTCCA 
CTGGTCACAC AAGCAGCCGA CTTTTTCAAG TCAAAACTTC CCCAAGCCTC CCCCGTCTCC 
CCTCTGGCCT TCCCTCACTG TCTGCAGGCT CCCATTCAGA GGCTGGAGCA GTACTGCGAG 
GCGCTGGAGG AGCTGGGAGG CATAAACCCC GCCTCAGACT CCGCCCTCTC CATCCTGAGA 
CACGCCCAGC GGCACGGCGA GGACCTCAGG GCCAGTGACC TCATCGTCGG ATGCCCGATC 
CCTGTGACGG AGCGCGGCGA GCTGGTGCGG CAGGGTGAGC TGACGGTGAG CGGGGGAACT 
CGGAGGAAGC GGGCGGGCGT GAGGAACGTT TTCCTTTATC AACACGTCGT AATCTTCACT 
AAACACAAGA ATCCCAGCCC GGGACGCACC GCCTACAGTT ACAAACACAG CATCAAGACG 
AATGAGATGG GTCTGACTCA GAGCGTCGGC GAGGAGGGAG TGAAGTTCGA GGTGTGGGTC 
CGACAGGCCC CGAGGACCAG AGACTGCATC ACCCTGCAAG CGCGCGACAG GGAAGACCGA 
GAGGCCTGGA CTCACGACAT AGCCCATCTG CTGTGGACAC ATGCCATCAA CAATACAGAG 
TTGTGTTTGA AGGAGTCACT GTGCATGGGA GTTTCCAGTA GGCTTCTGTT GGATGCAACA 
GGAACTCCAG GCTCAGAGTT GGACTCCATC TACAGTCTCA GCGACAGAAT CCACAGTAGC 
TGTTCAGACA CTTCTTCTGT CGGGAGTCAG AAGGAGGGGG GATCTCCAGC GTCTGGGAGG 
GACCCCAGGA GAAGCTCTGG ATCAACAAGC TGCTCACAGA GCCAGTCTCC CTCTACAGCT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004563633.2
CDS	177..5402
Translation	MCVYLCGSEAMEDCVQGALSSLYPPFESTAPPLLSQVFSVLESTYQHDSLRYLLDYFVPAKHLLHKLQQHACSQYLGCLFLHSGWPLCLGEKVVVQLSTLDWRLLRSNDFYLQVVPFSTRCPRLALKCLAPGGRTVQEILVPESQHPLVFTTEWLHIINKERGHKREVGGGLDTCLVSTCDGVVRLPWKEVVYPKFIHDPSEELGLMSNRLPSEGGSSLGGWGGSSSGELDSWSWDEEEDEDLQPDGLDSDCVLPRRRRSEDGLGRTSRQPQLDGDYVELLEPRGGPDGGVDPRQRYQEMHGICKTKTLPLCRRGKAIKVRRGKAWGYGRTERSGSFRAILGIKGDAVAAKGEALPPLPVPGVAEPGRGRRSFSSSVHDSDEGDKTNRDSEGLESRRLCFDGPFKERRPGVGKERDSNGHTQLCKDDHKSKESKSNNSLDTGEIHELTNHKDGHVVEGHSDHGSHSDSVFEDADKPLSGDSEANTPTSDAPQRPFAAVSKSGEITNTGSKKKSLSNQKSMDEKDEGKVEDRVCPRGKEVKTAGFRAPRRKRKGKGAKGKAKSGGRVKEKALKMQVKTPQPSPATCTASTKLPTAKESQTEEAEQADQTEGASAADSEATDNTVKSSAEAESSLPVCNGQSGTSFFNHSNEEAASPDKQNGDASKEPALLRELDADLLQSGKLKLTGTVDRLGRVLVFTEADASEVGFSGEEMARVLACYHTITRPAAKEKGLTVLMDSRRAPPSALLFSALKLFQGSVPGGLGSLLVLVEEQQESPSHSLEGAEVHTVRGTSVLQQYVDKQQLPKEVDGDFIHCHADWLAFRLSLESLTERCESALSLLGEALQSMDAEPMPDNIKAVPLSIDKHRQLMASVLADQRLTELQQKGGAWLAGLTNRASGLAQKSPDCRAALAVTSKLYDSVDDALHRLVRVSNQRGRDLEALGRLAGLVDKLEKCDKEIEQVQSQLEDYKDPPLSLSRLSLKQQRFKTFKETANELHSETLSLLSDLEGWCELDWAGLSNVQIRLPPVREKLRDMSHCLSDCWTTLDNTQRLLSTLTEATQWCDAVSSTSSSSSSSTCPLASLPPIPPSRFQDARSLAIELGGGALLDLWTQTVERYQRTVAQVKPRFLQSDRTQNQGQGKTKTPSASSLWDLMGPEGEGDSWGLGAGGGEGGLQSWGSLASLFRPQTCSTLKIGEEKGNKKEGTAGGSGAGGGGAGGGKFLSNLLNPTKKSPTEAPLPFKPPRKRHPSFDLQALLAPRRGTATPKPAESPVAGASRNSPMSWLGRRALADQVITTSMAAAIPGWSGGGSSGGGVLIRGVEVSSKEVVDHTGSPRQHVLLGRTDRETGTDRAGSTAQSKLYLLWCRMLSSERQYVTVLKGVEETYLPLLELSDTPASIRGKADSLFPNWASLTTFHSQDLLPAMEGALVQSLLQQDCFSKFRDQFLQYSQYIRTKPDLDSPLVTQAADFFKSKLPQASPVSPLAFPHCLQAPIQRLEQYCEALEELGGINPASDSALSILRHAQRHGEDLRASDLIVGCPIPVTERGELVRQGELTVSGGTRRKRAGVRNVFLYQHVVIFTKHKNPSPGRTAYSYKHSIKTNEMGLTQSVGEEGVKFEVWVRQAPRTRDCITLQARDREDREAWTHDIAHLLWTHAINNTELCLKESLCMGVSSRLLLDATGTPGSELDSIYSLSDRIHSSCSDTSSVGSQKEGGSPASGRDPRRSSGSTSCSQSQSPSTAV

Target ORF information:

RefSeq Version	XM_004563576.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004563576.3

ORF Insert Sequence:

ATGTGTGTAT ATTTATGTGG TTCAGAGGCA ATGGAGGACT GCGTGCAGGG GGCGCTCTCA 
TCGCTTTATC CTCCTTTTGA GAGCACAGCC CCACCCCTCC TCTCCCAGGT CTTCTCAGTC 
CTGGAGTCGA CCTATCAACA CGACAGTCTT CGCTATCTCC TGGACTACTT CGTACCTGCA 
AAGCACCTCC TGCACAAACT CCAGCAGCAT GCCTGTTCTC AGTACCTGGG TTGCTTGTTC 
CTTCACTCTG GCTGGCCTCT CTGCCTGGGC GAAAAAGTGG TTGTCCAACT TTCCACTTTG 
GACTGGAGGC TTCTGCGCAG TAATGACTTC TACCTACAGG TGGTTCCCTT CTCTACACGT 
TGCCCAAGGC TGGCACTAAA ATGCCTGGCT CCTGGGGGAC GGACTGTTCA GGAAATCTTG 
GTGCCCGAGT CACAGCATCC CCTTGTGTTC ACCACTGAGT GGCTTCACAT CATCAACAAA 
GAGCGAGGTC ACAAGAGAGA AGTTGGAGGA GGACTTGACA CCTGTCTGGT CAGTACATGC 
GATGGTGTGG TTAGACTTCC ATGGAAGGAG GTAGTCTACC CAAAATTTAT CCACGACCCC 
TCTGAAGAGT TGGGCCTAAT GTCCAACCGT CTGCCTAGCG AGGGGGGTAG CAGCCTCGGA 
GGCTGGGGTG GCTCATCCTC TGGGGAATTA GACTCCTGGT CCTGGGATGA AGAAGAAGAT 
GAGGATTTGC AACCGGATGG CTTGGACTCT GACTGTGTGC TTCCTCGACG AAGGCGCAGT 
GAAGACGGCC TTGGGCGGAC ATCGAGACAA CCTCAGCTCG ATGGGGATTA TGTCGAGCTT 
TTGGAACCCA GGGGGGGTCC TGATGGAGGA GTCGATCCAA GGCAGCGGTA CCAAGAGATG 
CATGGAATCT GTAAGACCAA GACTCTACCT CTTTGCAGGA GAGGAAAGGC TATCAAGGTT 
CGCAGAGGAA AAGCATGGGG ATATGGGCGA ACGGAAAGGT CAGGAAGCTT TCGGGCTATT 
CTTGGCATCA AAGGAGACGC TGTGGCTGCC AAGGGTGAAG CATTACCGCC ACTGCCTGTG 
CCAGGAGTTG CCGAGCCTGG CCGAGGGAGG AGGTCTTTCT CCTCCTCAGT CCATGACTCT 
GATGAAGGAG ATAAAACAAA CAGAGACTCT GAAGGCCTGG AAAGCCGCCG CCTTTGTTTT 
GATGGCCCAT TCAAGGAAAG GAGACCTGGA GTGGGCAAGG AGAGAGATAG CAATGGTCAC 
ACACAGCTAT GTAAAGATGA CCACAAAAGT AAAGAATCCA AGAGCAACAA CAGCTTGGAC 
ACAGGTGAAA TCCATGAGTT AACAAACCAC AAAGATGGAC ACGTTGTAGA GGGCCACTCT 
GATCACGGAT CACATTCGGA CTCTGTTTTT GAGGATGCTG ATAAACCACT GAGCGGAGAC 
AGTGAGGCTA ATACCCCAAC ATCAGATGCA CCACAGAGAC CATTCGCTGC TGTTTCAAAA 
AGTGGTGAAA TTACAAACAC TGGGTCTAAA AAGAAGAGTT TGTCAAACCA AAAATCCATG 
GATGAAAAGG ATGAAGGAAA AGTGGAGGAC AGAGTATGCC CCAGAGGAAA AGAGGTTAAA 
ACGGCAGGAT TCAGAGCACC AAGGAGGAAA AGGAAGGGAA AGGGGGCCAA AGGGAAAGCT 
AAATCTGGGG GTCGTGTAAA GGAGAAGGCC TTGAAAATGC AAGTTAAAAC TCCTCAACCA 
AGCCCAGCCA CCTGCACTGC TTCAACAAAA CTGCCGACTG CCAAGGAGAG CCAAACAGAG 
GAAGCAGAGC AGGCTGACCA AACTGAAGGT GCTTCTGCTG CAGATAGCGA GGCGACAGAC 
AACACAGTGA AAAGCAGCGC AGAAGCTGAA TCATCTCTGC CTGTGTGTAA CGGACAGTCG 
GGCACATCTT TTTTTAACCA CTCTAATGAG GAGGCTGCGT CACCTGACAA ACAGAATGGT 
GATGCTTCAA AAGAGCCCGC CCTGCTGAGG GAACTGGACG CAGACCTTCT CCAGTCAGGG 
AAGCTCAAGT TGACAGGTAC AGTGGACAGG CTGGGGCGTG TTCTGGTTTT CACAGAGGCC 
GACGCATCAG AAGTGGGTTT CTCTGGGGAG GAGATGGCCC GCGTCTTGGC CTGTTACCAC 
ACAATCACGC GGCCTGCAGC CAAAGAAAAG GGTTTAACAG TGTTAATGGA CAGCAGGCGC 
GCCCCACCCT CCGCTCTCTT GTTCTCTGCT CTTAAATTGT TCCAGGGGTC GGTCCCCGGT 
GGTCTTGGAT CCCTTCTGGT GTTGGTGGAA GAGCAGCAGG AGTCCCCTTC TCATTCCCTG 
GAAGGAGCAG AGGTCCACAC AGTGCGAGGA ACGAGTGTCC TTCAGCAGTA TGTAGACAAA 
CAACAGCTGC CCAAAGAAGT AGACGGGGAC TTTATCCACT GCCACGCGGA CTGGCTGGCC 
TTCAGACTGA GTCTGGAGAG TCTGACAGAG CGCTGTGAGA GCGCCCTCTC ACTGCTTGGA 
GAAGCACTGC AGTCTATGGA CGCTGAGCCA ATGCCAGACA ACATAAAGGC TGTTCCTCTG 
AGCATTGACA AACACAGGCA GCTCATGGCC AGCGTCCTGG CCGACCAACG GTTAACTGAG 
CTGCAGCAGA AGGGCGGGGC CTGGCTTGCT GGATTGACAA ATAGAGCATC TGGACTGGCA 
CAAAAGTCAC CAGACTGCAG AGCTGCTCTT GCCGTAACTT CTAAACTTTA TGACAGCGTG 
GACGATGCAC TCCATCGGCT TGTCCGAGTG TCCAACCAAA GAGGTCGTGA CCTGGAGGCG 
TTGGGGCGTC TGGCGGGGTT GGTGGACAAA CTGGAAAAGT GCGATAAAGA GATTGAGCAG 
GTTCAGTCGC AGCTGGAGGA CTACAAGGAT CCTCCTCTCT CCCTCAGCAG ACTCTCGCTC 
AAACAGCAGA GGTTCAAGAC GTTCAAAGAA ACGGCCAATG AGCTGCACAG TGAGACTCTA 
TCACTGCTCA GTGATTTGGA GGGCTGGTGT GAACTGGACT GGGCAGGCCT GAGCAACGTC 
CAGATCCGAC TCCCTCCGGT CAGGGAGAAG CTCAGGGACA TGTCCCACTG TCTGTCTGAC 
TGCTGGACCA CTCTGGACAA CACGCAGAGG TTGTTGTCCA CTCTGACCGA GGCCACCCAG 
TGGTGCGATG CAGTCTCCTC CACCTCCTCC TCTTCCTCTT CTTCCACCTG CCCTCTCGCC 
TCCCTCCCTC CAATACCCCC GTCCCGTTTC CAGGACGCAC GCTCTTTAGC AATAGAGCTG 
GGTGGCGGGG CACTGCTGGA TCTCTGGACT CAGACAGTTG AGCGATACCA GCGAACAGTA 
GCACAGGTCA AGCCCCGATT CCTCCAGTCT GACCGGACGC AAAATCAGGG ACAGGGGAAA 
ACCAAAACTC CCTCTGCCAG CAGCCTCTGG GACCTGATGG GCCCCGAGGG AGAGGGTGAC 
TCCTGGGGGC TGGGAGCTGG CGGAGGGGAA GGCGGATTGC AGTCCTGGGG ATCCCTGGCA 
TCACTGTTCA GACCGCAGAC CTGCTCCACA CTGAAGATAG GAGAGGAGAA AGGGAATAAG 
AAAGAGGGAA CGGCAGGAGG AAGTGGTGCG GGAGGAGGTG GGGCCGGGGG AGGGAAGTTT 
CTATCGAACC TTCTGAATCC TACAAAGAAG AGCCCCACAG AGGCTCCACT TCCCTTCAAG 
CCACCTCGGA AGCGTCACCC AAGCTTTGAC CTCCAGGCTC TCCTGGCCCC CCGTCGAGGT 
ACAGCCACCC CCAAACCCGC TGAGTCGCCG GTGGCTGGGG CGAGCCGTAA CTCCCCCATG 
TCCTGGCTGG GACGACGGGC TTTGGCCGAC CAAGTCATTA CTACTAGCAT GGCTGCAGCT 
ATTCCAGGAT GGAGCGGTGG TGGTAGTAGT GGCGGAGGAG TGTTGATCCG AGGAGTGGAG 
GTCAGCAGCA AGGAGGTGGT GGATCACACG GGGTCACCGC GCCAGCATGT ACTGCTGGGG 
AGGACAGACA GGGAGACGGG GACGGATAGG GCCGGTTCAA CCGCACAGAG TAAGCTGTAC 
CTGCTCTGGT GTAGGATGCT AAGTTCAGAG AGGCAGTACG TGACTGTGCT AAAGGGGGTA 
GAAGAGACGT ACCTGCCCTT ACTGGAGCTC TCAGACACCC CAGCTTCCAT CAGGGGGAAG 
GCAGACTCGC TGTTTCCCAA CTGGGCCAGC CTCACCACCT TTCACTCGCA GGACCTACTC 
CCAGCCATGG AGGGCGCTCT TGTGCAAAGC CTGTTGCAAC AGGACTGCTT CAGTAAATTT 
CGGGACCAGT TTCTGCAGTA TTCCCAGTAC ATCAGGACCA AACCAGACCT GGATTCTCCA 
CTGGTCACAC AAGCAGCCGA CTTTTTCAAG TCAAAACTTC CCCAAGCCTC CCCCGTCTCC 
CCTCTGGCCT TCCCTCACTG TCTGCAGGCT CCCATTCAGA GGCTGGAGCA GTACTGCGAG 
GCGCTGGAGG AGCTGGGAGG CATAAACCCC GCCTCAGACT CCGCCCTCTC CATCCTGAGA 
CACGCCCAGC GGCACGGCGA GGACCTCAGG GCCAGTGACC TCATCGTCGG ATGCCCGATC 
CCTGTGACGG AGCGCGGCGA GCTGGTGCGG CAGGGTGAGC TGACGGTGAG CGGGGGAACT 
CGGAGGAAGC GGGCGGGCGT GAGGAACGTT TTCCTTTATC AACACGTCGT AATCTTCACT 
AAACACAAGA ATCCCAGCCC GGGACGCACC GCCTACAGTT ACAAACACAG CATCAAGACG 
AATGAGATGG GTCTGACTCA GAGCGTCGGC GAGGAGGGAG TGAAGTTCGA GGTGTGGGTC 
CGACAGGCCC CGAGGACCAG AGACTGCATC ACCCTGCAAG CGCGCGACAG GGAAGACCGA 
GAGGCCTGGA CTCACGACAT AGCCCATCTG CTGTGGACAC ATGCCATCAA CAATACAGAG 
TTGTGTTTGA AGGAGTCACT GTGCATGGGA GTTTCCAGTA GGCTTCTGTT GGATGCAACA 
GGAACTCCAG GCTCAGAGTT GGACTCCATC TACAGTCTCA GCGACAGAAT CCACAGTAGC 
TGTTCAGACA CTTCTTCTGT CGGGAGTCAG AAGGAGGGGG GATCTCCAGC GTCTGGGAGG 
GACCCCAGGA GAAGCTCTGG ATCAACAAGC TGCTCACAGA GCCAGTCTCC CTCTACAGCT 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477218 ( XM_023153878.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009646.2 Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X2, mRNA.

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CloneID	OMa370663
Clone ID Related Accession (Same CDS sequence)	XM_023153878.2
Accession Version	XM_023153878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	pleckstrin homology domain-containing family G member 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCAG AGGCAATGGA GGACTGCGTG CAGGGGGCGC TCTCATCGCT TTATCCTCCT 
TTTGAGAGCA CAGCCCCACC CCTCCTCTCC CAGGTCTTCT CAGTCCTGGA GTCGACCTAT 
CAACACGACA GTCTTCGCTA TCTCCTGGAC TACTTCGTAC CTGCAAAGCA CCTCCTGCAC 
AAACTCCAGC AGCATGCCTG TTCTCAGTAC CTGGGTTGCT TGTTCCTTCA CTCTGGCTGG 
CCTCTCTGCC TGGGCGAAAA AGTGGTTGTC CAACTTTCCA CTTTGGACTG GAGGCTTCTG 
CGCAGTAATG ACTTCTACCT ACAGGTGGTT CCCTTCTCTA CACGTTGCCC AAGGCTGGCA 
CTAAAATGCC TGGCTCCTGG GGGACGGACT GTTCAGGAAA TCTTGGTGCC CGAGTCACAG 
CATCCCCTTG TGTTCACCAC TGAGTGGCTT CACATCATCA ACAAAGAGCG AGGTCACAAG 
AGAGAAGTTG GAGGAGGACT TGACACCTGT CTGGTCAGTA CATGCGATGG TGTGGTTAGA 
CTTCCATGGA AGGAGGTAGT CTACCCAAAA TTTATCCACG ACCCCTCTGA AGAGTTGGGC 
CTAATGTCCA ACCGTCTGCC TAGCGAGGGG GGTAGCAGCC TCGGAGGCTG GGGTGGCTCA 
TCCTCTGGGG AATTAGACTC CTGGTCCTGG GATGAAGAAG AAGATGAGGA TTTGCAACCG 
GATGGCTTGG ACTCTGACTG TGTGCTTCCT CGACGAAGGC GCAGTGAAGA CGGCCTTGGG 
CGGACATCGA GACAACCTCA GCTCGATGGG GATTATGTCG AGCTTTTGGA ACCCAGGGGG 
GGTCCTGATG GAGGAGTCGA TCCAAGGCAG CGGTACCAAG AGATGCATGG AATCTGTAAG 
ACCAAGACTC TACCTCTTTG CAGGAGAGGA AAGGCTATCA AGGTTCGCAG AGGAAAAGCA 
TGGGGATATG GGCGAACGGA AAGGTCAGGA AGCTTTCGGG CTATTCTTGG CATCAAAGGA 
GACGCTGTGG CTGCCAAGGG TGAAGCATTA CCGCCACTGC CTGTGCCAGG AGTTGCCGAG 
CCTGGCCGAG GGAGGAGGTC TTTCTCCTCC TCAGTCCATG ACTCTGATGA AGGAGATAAA 
ACAAACAGAG ACTCTGAAGG CCTGGAAAGC CGCCGCCTTT GTTTTGATGG CCCATTCAAG 
GAAAGGAGAC CTGGAGTGGG CAAGGAGAGA GATAGCAATG GTCACACACA GCTATGTAAA 
GATGACCACA AAAGTAAAGA ATCCAAGAGC AACAACAGCT TGGACACAGG TGAAATCCAT 
GAGTTAACAA ACCACAAAGA TGGACACGTT GTAGAGGGCC ACTCTGATCA CGGATCACAT 
TCGGACTCTG TTTTTGAGGA TGCTGATAAA CCACTGAGCG GAGACAGTGA GGCTAATACC 
CCAACATCAG ATGCACCACA GAGACCATTC GCTGCTGTTT CAAAAAGTGG TGAAATTACA 
AACACTGGGT CTAAAAAGAA GAGTTTGTCA AACCAAAAAT CCATGGATGA AAAGGATGAA 
GGAAAAGTGG AGGACAGAGT ATGCCCCAGA GGAAAAGAGG TTAAAACGGC AGGATTCAGA 
GCACCAAGGA GGAAAAGGAA GGGAAAGGGG GCCAAAGGGA AAGCTAAATC TGGGGGTCGT 
GTAAAGGAGA AGGCCTTGAA AATGCAAGTT AAAACTCCTC AACCAAGCCC AGCCACCTGC 
ACTGCTTCAA CAAAACTGCC GACTGCCAAG GAGAGCCAAA CAGAGGAAGC AGAGCAGGCT 
GACCAAACTG AAGGTGCTTC TGCTGCAGAT AGCGAGGCGA CAGACAACAC AGTGAAAAGC 
AGCGCAGAAG CTGAATCATC TCTGCCTGTG TGTAACGGAC AGTCGGGCAC ATCTTTTTTT 
AACCACTCTA ATGAGGAGGC TGCGTCACCT GACAAACAGA ATGGTGATGC TTCAAAAGAG 
CCCGCCCTGC TGAGGGAACT GGACGCAGAC CTTCTCCAGT CAGGGAAGCT CAAGTTGACA 
GGTACAGTGG ACAGGCTGGG GCGTGTTCTG GTTTTCACAG AGGCCGACGC ATCAGAAGTG 
GGTTTCTCTG GGGAGGAGAT GGCCCGCGTC TTGGCCTGTT ACCACACAAT CACGCGGCCT 
GCAGCCAAAG AAAAGGGTTT AACAGTGTTA ATGGACAGCA GGCGCGCCCC ACCCTCCGCT 
CTCTTGTTCT CTGCTCTTAA ATTGTTCCAG GGGTCGGTCC CCGGTGGTCT TGGATCCCTT 
CTGGTGTTGG TGGAAGAGCA GCAGGAGTCC CCTTCTCATT CCCTGGAAGG AGCAGAGGTC 
CACACAGTGC GAGGAACGAG TGTCCTTCAG CAGTATGTAG ACAAACAACA GCTGCCCAAA 
GAAGTAGACG GGGACTTTAT CCACTGCCAC GCGGACTGGC TGGCCTTCAG ACTGAGTCTG 
GAGAGTCTGA CAGAGCGCTG TGAGAGCGCC CTCTCACTGC TTGGAGAAGC ACTGCAGTCT 
ATGGACGCTG AGCCAATGCC AGACAACATA AAGGCTGTTC CTCTGAGCAT TGACAAACAC 
AGGCAGCTCA TGGCCAGCGT CCTGGCCGAC CAACGGTTAA CTGAGCTGCA GCAGAAGGGC 
GGGGCCTGGC TTGCTGGATT GACAAATAGA GCATCTGGAC TGGCACAAAA GTCACCAGAC 
TGCAGAGCTG CTCTTGCCGT AACTTCTAAA CTTTATGACA GCGTGGACGA TGCACTCCAT 
CGGCTTGTCC GAGTGTCCAA CCAAAGAGGT CGTGACCTGG AGGCGTTGGG GCGTCTGGCG 
GGGTTGGTGG ACAAACTGGA AAAGTGCGAT AAAGAGATTG AGCAGGTTCA GTCGCAGCTG 
GAGGACTACA AGGATCCTCC TCTCTCCCTC AGCAGACTCT CGCTCAAACA GCAGAGGTTC 
AAGACGTTCA AAGAAACGGC CAATGAGCTG CACAGTGAGA CTCTATCACT GCTCAGTGAT 
TTGGAGGGCT GGTGTGAACT GGACTGGGCA GGCCTGAGCA ACGTCCAGAT CCGACTCCCT 
CCGGTCAGGG AGAAGCTCAG GGACATGTCC CACTGTCTGT CTGACTGCTG GACCACTCTG 
GACAACACGC AGAGGTTGTT GTCCACTCTG ACCGAGGCCA CCCAGTGGTG CGATGCAGTC 
TCCTCCACCT CCTCCTCTTC CTCTTCTTCC ACCTGCCCTC TCGCCTCCCT CCCTCCAATA 
CCCCCGTCCC GTTTCCAGGA CGCACGCTCT TTAGCAATAG AGCTGGGTGG CGGGGCACTG 
CTGGATCTCT GGACTCAGAC AGTTGAGCGA TACCAGCGAA CAGTAGCACA GGTCAAGCCC 
CGATTCCTCC AGTCTGACCG GACGCAAAAT CAGGGACAGG GGAAAACCAA AACTCCCTCT 
GCCAGCAGCC TCTGGGACCT GATGGGCCCC GAGGGAGAGG GTGACTCCTG GGGGCTGGGA 
GCTGGCGGAG GGGAAGGCGG ATTGCAGTCC TGGGGATCCC TGGCATCACT GTTCAGACCG 
CAGACCTGCT CCACACTGAA GATAGGAGAG GAGAAAGGGA ATAAGAAAGA GGGAACGGCA 
GGAGGAAGTG GTGCGGGAGG AGGTGGGGCC GGGGGAGGGA AGTTTCTATC GAACCTTCTG 
AATCCTACAA AGAAGAGCCC CACAGAGGCT CCACTTCCCT TCAAGCCACC TCGGAAGCGT 
CACCCAAGCT TTGACCTCCA GGCTCTCCTG GCCCCCCGTC GAGGTACAGC CACCCCCAAA 
CCCGCTGAGT CGCCGGTGGC TGGGGCGAGC CGTAACTCCC CCATGTCCTG GCTGGGACGA 
CGGGCTTTGG CCGACCAAGT CATTACTACT AGCATGGCTG CAGCTATTCC AGGATGGAGC 
GGTGGTGGTA GTAGTGGCGG AGGAGTGTTG ATCCGAGGAG TGGAGGTCAG CAGCAAGGAG 
GTGGTGGATC ACACGGGGTC ACCGCGCCAG CATGTACTGC TGGGGAGGAC AGACAGGGAG 
ACGGGGACGG ATAGGGCCGG TTCAACCGCA CAGAGTAAGC TGTACCTGCT CTGGTGTAGG 
ATGCTAAGTT CAGAGAGGCA GTACGTGACT GTGCTAAAGG GGGTAGAAGA GACGTACCTG 
CCCTTACTGG AGCTCTCAGA CACCCCAGCT TCCATCAGGG GGAAGGCAGA CTCGCTGTTT 
CCCAACTGGG CCAGCCTCAC CACCTTTCAC TCGCAGGACC TACTCCCAGC CATGGAGGGC 
GCTCTTGTGC AAAGCCTGTT GCAACAGGAC TGCTTCAGTA AATTTCGGGA CCAGTTTCTG 
CAGTATTCCC AGTACATCAG GACCAAACCA GACCTGGATT CTCCACTGGT CACACAAGCA 
GCCGACTTTT TCAAGTCAAA ACTTCCCCAA GCCTCCCCCG TCTCCCCTCT GGCCTTCCCT 
CACTGTCTGC AGGCTCCCAT TCAGAGGCTG GAGCAGTACT GCGAGGCGCT GGAGGAGCTG 
GGAGGCATAA ACCCCGCCTC AGACTCCGCC CTCTCCATCC TGAGACACGC CCAGCGGCAC 
GGCGAGGACC TCAGGGCCAG TGACCTCATC GTCGGATGCC CGATCCCTGT GACGGAGCGC 
GGCGAGCTGG TGCGGCAGGG TGAGCTGACG GTGAGCGGGG GAACTCGGAG GAAGCGGGCG 
GGCGTGAGGA ACGTTTTCCT TTATCAACAC GTCGTAATCT TCACTAAACA CAAGAATCCC 
AGCCCGGGAC GCACCGCCTA CAGTTACAAA CACAGCATCA AGACGAATGA GATGGGTCTG 
ACTCAGAGCG TCGGCGAGGA GGGAGTGAAG TTCGAGGTGT GGGTCCGACA GGCCCCGAGG 
ACCAGAGACT GCATCACCCT GCAAGCGCGC GACAGGGAAG ACCGAGAGGC CTGGACTCAC 
GACATAGCCC ATCTGCTGTG GACACATGCC ATCAACAATA CAGAGTTGTG TTTGAAGGAG 
TCACTGTGCA TGGGAGTTTC CAGTAGGCTT CTGTTGGATG CAACAGGAAC TCCAGGCTCA 
GAGTTGGACT CCATCTACAG TCTCAGCGAC AGAATCCACA GTAGCTGTTC AGACACTTCT 
TCTGTCGGGA GTCAGAAGGA GGGGGGATCT CCAGCGTCTG GGAGGGACCC CAGGAGAAGC 
TCTGGATCAA CAAGCTGCTC ACAGAGCCAG TCTCCCTCTA CAGCTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009646.2
CDS	247..5457
Translation	MGSEAMEDCVQGALSSLYPPFESTAPPLLSQVFSVLESTYQHDSLRYLLDYFVPAKHLLHKLQQHACSQYLGCLFLHSGWPLCLGEKVVVQLSTLDWRLLRSNDFYLQVVPFSTRCPRLALKCLAPGGRTVQEILVPESQHPLVFTTEWLHIINKERGHKREVGGGLDTCLVSTCDGVVRLPWKEVVYPKFIHDPSEELGLMSNRLPSEGGSSLGGWGGSSSGELDSWSWDEEEDEDLQPDGLDSDCVLPRRRRSEDGLGRTSRQPQLDGDYVELLEPRGGPDGGVDPRQRYQEMHGICKTKTLPLCRRGKAIKVRRGKAWGYGRTERSGSFRAILGIKGDAVAAKGEALPPLPVPGVAEPGRGRRSFSSSVHDSDEGDKTNRDSEGLESRRLCFDGPFKERRPGVGKERDSNGHTQLCKDDHKSKESKSNNSLDTGEIHELTNHKDGHVVEGHSDHGSHSDSVFEDADKPLSGDSEANTPTSDAPQRPFAAVSKSGEITNTGSKKKSLSNQKSMDEKDEGKVEDRVCPRGKEVKTAGFRAPRRKRKGKGAKGKAKSGGRVKEKALKMQVKTPQPSPATCTASTKLPTAKESQTEEAEQADQTEGASAADSEATDNTVKSSAEAESSLPVCNGQSGTSFFNHSNEEAASPDKQNGDASKEPALLRELDADLLQSGKLKLTGTVDRLGRVLVFTEADASEVGFSGEEMARVLACYHTITRPAAKEKGLTVLMDSRRAPPSALLFSALKLFQGSVPGGLGSLLVLVEEQQESPSHSLEGAEVHTVRGTSVLQQYVDKQQLPKEVDGDFIHCHADWLAFRLSLESLTERCESALSLLGEALQSMDAEPMPDNIKAVPLSIDKHRQLMASVLADQRLTELQQKGGAWLAGLTNRASGLAQKSPDCRAALAVTSKLYDSVDDALHRLVRVSNQRGRDLEALGRLAGLVDKLEKCDKEIEQVQSQLEDYKDPPLSLSRLSLKQQRFKTFKETANELHSETLSLLSDLEGWCELDWAGLSNVQIRLPPVREKLRDMSHCLSDCWTTLDNTQRLLSTLTEATQWCDAVSSTSSSSSSSTCPLASLPPIPPSRFQDARSLAIELGGGALLDLWTQTVERYQRTVAQVKPRFLQSDRTQNQGQGKTKTPSASSLWDLMGPEGEGDSWGLGAGGGEGGLQSWGSLASLFRPQTCSTLKIGEEKGNKKEGTAGGSGAGGGGAGGGKFLSNLLNPTKKSPTEAPLPFKPPRKRHPSFDLQALLAPRRGTATPKPAESPVAGASRNSPMSWLGRRALADQVITTSMAAAIPGWSGGGSSGGGVLIRGVEVSSKEVVDHTGSPRQHVLLGRTDRETGTDRAGSTAQSKLYLLWCRMLSSERQYVTVLKGVEETYLPLLELSDTPASIRGKADSLFPNWASLTTFHSQDLLPAMEGALVQSLLQQDCFSKFRDQFLQYSQYIRTKPDLDSPLVTQAADFFKSKLPQASPVSPLAFPHCLQAPIQRLEQYCEALEELGGINPASDSALSILRHAQRHGEDLRASDLIVGCPIPVTERGELVRQGELTVSGGTRRKRAGVRNVFLYQHVVIFTKHKNPSPGRTAYSYKHSIKTNEMGLTQSVGEEGVKFEVWVRQAPRTRDCITLQARDREDREAWTHDIAHLLWTHAINNTELCLKESLCMGVSSRLLLDATGTPGSELDSIYSLSDRIHSSCSDTSSVGSQKEGGSPASGRDPRRSSGSTSCSQSQSPSTAV

Target ORF information:

RefSeq Version	XM_023153878.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra pleckstrin homology domain-containing family G member 4B (LOC101477218), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153878.2

ORF Insert Sequence:

ATGGGTTCAG AGGCAATGGA GGACTGCGTG CAGGGGGCGC TCTCATCGCT TTATCCTCCT 
TTTGAGAGCA CAGCCCCACC CCTCCTCTCC CAGGTCTTCT CAGTCCTGGA GTCGACCTAT 
CAACACGACA GTCTTCGCTA TCTCCTGGAC TACTTCGTAC CTGCAAAGCA CCTCCTGCAC 
AAACTCCAGC AGCATGCCTG TTCTCAGTAC CTGGGTTGCT TGTTCCTTCA CTCTGGCTGG 
CCTCTCTGCC TGGGCGAAAA AGTGGTTGTC CAACTTTCCA CTTTGGACTG GAGGCTTCTG 
CGCAGTAATG ACTTCTACCT ACAGGTGGTT CCCTTCTCTA CACGTTGCCC AAGGCTGGCA 
CTAAAATGCC TGGCTCCTGG GGGACGGACT GTTCAGGAAA TCTTGGTGCC CGAGTCACAG 
CATCCCCTTG TGTTCACCAC TGAGTGGCTT CACATCATCA ACAAAGAGCG AGGTCACAAG 
AGAGAAGTTG GAGGAGGACT TGACACCTGT CTGGTCAGTA CATGCGATGG TGTGGTTAGA 
CTTCCATGGA AGGAGGTAGT CTACCCAAAA TTTATCCACG ACCCCTCTGA AGAGTTGGGC 
CTAATGTCCA ACCGTCTGCC TAGCGAGGGG GGTAGCAGCC TCGGAGGCTG GGGTGGCTCA 
TCCTCTGGGG AATTAGACTC CTGGTCCTGG GATGAAGAAG AAGATGAGGA TTTGCAACCG 
GATGGCTTGG ACTCTGACTG TGTGCTTCCT CGACGAAGGC GCAGTGAAGA CGGCCTTGGG 
CGGACATCGA GACAACCTCA GCTCGATGGG GATTATGTCG AGCTTTTGGA ACCCAGGGGG 
GGTCCTGATG GAGGAGTCGA TCCAAGGCAG CGGTACCAAG AGATGCATGG AATCTGTAAG 
ACCAAGACTC TACCTCTTTG CAGGAGAGGA AAGGCTATCA AGGTTCGCAG AGGAAAAGCA 
TGGGGATATG GGCGAACGGA AAGGTCAGGA AGCTTTCGGG CTATTCTTGG CATCAAAGGA 
GACGCTGTGG CTGCCAAGGG TGAAGCATTA CCGCCACTGC CTGTGCCAGG AGTTGCCGAG 
CCTGGCCGAG GGAGGAGGTC TTTCTCCTCC TCAGTCCATG ACTCTGATGA AGGAGATAAA 
ACAAACAGAG ACTCTGAAGG CCTGGAAAGC CGCCGCCTTT GTTTTGATGG CCCATTCAAG 
GAAAGGAGAC CTGGAGTGGG CAAGGAGAGA GATAGCAATG GTCACACACA GCTATGTAAA 
GATGACCACA AAAGTAAAGA ATCCAAGAGC AACAACAGCT TGGACACAGG TGAAATCCAT 
GAGTTAACAA ACCACAAAGA TGGACACGTT GTAGAGGGCC ACTCTGATCA CGGATCACAT 
TCGGACTCTG TTTTTGAGGA TGCTGATAAA CCACTGAGCG GAGACAGTGA GGCTAATACC 
CCAACATCAG ATGCACCACA GAGACCATTC GCTGCTGTTT CAAAAAGTGG TGAAATTACA 
AACACTGGGT CTAAAAAGAA GAGTTTGTCA AACCAAAAAT CCATGGATGA AAAGGATGAA 
GGAAAAGTGG AGGACAGAGT ATGCCCCAGA GGAAAAGAGG TTAAAACGGC AGGATTCAGA 
GCACCAAGGA GGAAAAGGAA GGGAAAGGGG GCCAAAGGGA AAGCTAAATC TGGGGGTCGT 
GTAAAGGAGA AGGCCTTGAA AATGCAAGTT AAAACTCCTC AACCAAGCCC AGCCACCTGC 
ACTGCTTCAA CAAAACTGCC GACTGCCAAG GAGAGCCAAA CAGAGGAAGC AGAGCAGGCT 
GACCAAACTG AAGGTGCTTC TGCTGCAGAT AGCGAGGCGA CAGACAACAC AGTGAAAAGC 
AGCGCAGAAG CTGAATCATC TCTGCCTGTG TGTAACGGAC AGTCGGGCAC ATCTTTTTTT 
AACCACTCTA ATGAGGAGGC TGCGTCACCT GACAAACAGA ATGGTGATGC TTCAAAAGAG 
CCCGCCCTGC TGAGGGAACT GGACGCAGAC CTTCTCCAGT CAGGGAAGCT CAAGTTGACA 
GGTACAGTGG ACAGGCTGGG GCGTGTTCTG GTTTTCACAG AGGCCGACGC ATCAGAAGTG 
GGTTTCTCTG GGGAGGAGAT GGCCCGCGTC TTGGCCTGTT ACCACACAAT CACGCGGCCT 
GCAGCCAAAG AAAAGGGTTT AACAGTGTTA ATGGACAGCA GGCGCGCCCC ACCCTCCGCT 
CTCTTGTTCT CTGCTCTTAA ATTGTTCCAG GGGTCGGTCC CCGGTGGTCT TGGATCCCTT 
CTGGTGTTGG TGGAAGAGCA GCAGGAGTCC CCTTCTCATT CCCTGGAAGG AGCAGAGGTC 
CACACAGTGC GAGGAACGAG TGTCCTTCAG CAGTATGTAG ACAAACAACA GCTGCCCAAA 
GAAGTAGACG GGGACTTTAT CCACTGCCAC GCGGACTGGC TGGCCTTCAG ACTGAGTCTG 
GAGAGTCTGA CAGAGCGCTG TGAGAGCGCC CTCTCACTGC TTGGAGAAGC ACTGCAGTCT 
ATGGACGCTG AGCCAATGCC AGACAACATA AAGGCTGTTC CTCTGAGCAT TGACAAACAC 
AGGCAGCTCA TGGCCAGCGT CCTGGCCGAC CAACGGTTAA CTGAGCTGCA GCAGAAGGGC 
GGGGCCTGGC TTGCTGGATT GACAAATAGA GCATCTGGAC TGGCACAAAA GTCACCAGAC 
TGCAGAGCTG CTCTTGCCGT AACTTCTAAA CTTTATGACA GCGTGGACGA TGCACTCCAT 
CGGCTTGTCC GAGTGTCCAA CCAAAGAGGT CGTGACCTGG AGGCGTTGGG GCGTCTGGCG 
GGGTTGGTGG ACAAACTGGA AAAGTGCGAT AAAGAGATTG AGCAGGTTCA GTCGCAGCTG 
GAGGACTACA AGGATCCTCC TCTCTCCCTC AGCAGACTCT CGCTCAAACA GCAGAGGTTC 
AAGACGTTCA AAGAAACGGC CAATGAGCTG CACAGTGAGA CTCTATCACT GCTCAGTGAT 
TTGGAGGGCT GGTGTGAACT GGACTGGGCA GGCCTGAGCA ACGTCCAGAT CCGACTCCCT 
CCGGTCAGGG AGAAGCTCAG GGACATGTCC CACTGTCTGT CTGACTGCTG GACCACTCTG 
GACAACACGC AGAGGTTGTT GTCCACTCTG ACCGAGGCCA CCCAGTGGTG CGATGCAGTC 
TCCTCCACCT CCTCCTCTTC CTCTTCTTCC ACCTGCCCTC TCGCCTCCCT CCCTCCAATA 
CCCCCGTCCC GTTTCCAGGA CGCACGCTCT TTAGCAATAG AGCTGGGTGG CGGGGCACTG 
CTGGATCTCT GGACTCAGAC AGTTGAGCGA TACCAGCGAA CAGTAGCACA GGTCAAGCCC 
CGATTCCTCC AGTCTGACCG GACGCAAAAT CAGGGACAGG GGAAAACCAA AACTCCCTCT 
GCCAGCAGCC TCTGGGACCT GATGGGCCCC GAGGGAGAGG GTGACTCCTG GGGGCTGGGA 
GCTGGCGGAG GGGAAGGCGG ATTGCAGTCC TGGGGATCCC TGGCATCACT GTTCAGACCG 
CAGACCTGCT CCACACTGAA GATAGGAGAG GAGAAAGGGA ATAAGAAAGA GGGAACGGCA 
GGAGGAAGTG GTGCGGGAGG AGGTGGGGCC GGGGGAGGGA AGTTTCTATC GAACCTTCTG 
AATCCTACAA AGAAGAGCCC CACAGAGGCT CCACTTCCCT TCAAGCCACC TCGGAAGCGT 
CACCCAAGCT TTGACCTCCA GGCTCTCCTG GCCCCCCGTC GAGGTACAGC CACCCCCAAA 
CCCGCTGAGT CGCCGGTGGC TGGGGCGAGC CGTAACTCCC CCATGTCCTG GCTGGGACGA 
CGGGCTTTGG CCGACCAAGT CATTACTACT AGCATGGCTG CAGCTATTCC AGGATGGAGC 
GGTGGTGGTA GTAGTGGCGG AGGAGTGTTG ATCCGAGGAG TGGAGGTCAG CAGCAAGGAG 
GTGGTGGATC ACACGGGGTC ACCGCGCCAG CATGTACTGC TGGGGAGGAC AGACAGGGAG 
ACGGGGACGG ATAGGGCCGG TTCAACCGCA CAGAGTAAGC TGTACCTGCT CTGGTGTAGG 
ATGCTAAGTT CAGAGAGGCA GTACGTGACT GTGCTAAAGG GGGTAGAAGA GACGTACCTG 
CCCTTACTGG AGCTCTCAGA CACCCCAGCT TCCATCAGGG GGAAGGCAGA CTCGCTGTTT 
CCCAACTGGG CCAGCCTCAC CACCTTTCAC TCGCAGGACC TACTCCCAGC CATGGAGGGC 
GCTCTTGTGC AAAGCCTGTT GCAACAGGAC TGCTTCAGTA AATTTCGGGA CCAGTTTCTG 
CAGTATTCCC AGTACATCAG GACCAAACCA GACCTGGATT CTCCACTGGT CACACAAGCA 
GCCGACTTTT TCAAGTCAAA ACTTCCCCAA GCCTCCCCCG TCTCCCCTCT GGCCTTCCCT 
CACTGTCTGC AGGCTCCCAT TCAGAGGCTG GAGCAGTACT GCGAGGCGCT GGAGGAGCTG 
GGAGGCATAA ACCCCGCCTC AGACTCCGCC CTCTCCATCC TGAGACACGC CCAGCGGCAC 
GGCGAGGACC TCAGGGCCAG TGACCTCATC GTCGGATGCC CGATCCCTGT GACGGAGCGC 
GGCGAGCTGG TGCGGCAGGG TGAGCTGACG GTGAGCGGGG GAACTCGGAG GAAGCGGGCG 
GGCGTGAGGA ACGTTTTCCT TTATCAACAC GTCGTAATCT TCACTAAACA CAAGAATCCC 
AGCCCGGGAC GCACCGCCTA CAGTTACAAA CACAGCATCA AGACGAATGA GATGGGTCTG 
ACTCAGAGCG TCGGCGAGGA GGGAGTGAAG TTCGAGGTGT GGGTCCGACA GGCCCCGAGG 
ACCAGAGACT GCATCACCCT GCAAGCGCGC GACAGGGAAG ACCGAGAGGC CTGGACTCAC 
GACATAGCCC ATCTGCTGTG GACACATGCC ATCAACAATA CAGAGTTGTG TTTGAAGGAG 
TCACTGTGCA TGGGAGTTTC CAGTAGGCTT CTGTTGGATG CAACAGGAAC TCCAGGCTCA 
GAGTTGGACT CCATCTACAG TCTCAGCGAC AGAATCCACA GTAGCTGTTC AGACACTTCT 
TCTGTCGGGA GTCAGAAGGA GGGGGGATCT CCAGCGTCTG GGAGGGACCC CAGGAGAAGC 
TCTGGATCAA CAAGCTGCTC ACAGAGCCAG TCTCCCTCTA CAGCTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101477218 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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