LOC101477194 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101477194
Entrez Gene ID	101477194
Full Name	serine/threonine-protein kinase WNK4
Synonyms	wnk4
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014408459.3	XP_014263945.2	serine/threonine-protein kinase WNK4 isoform X1
XM_014408459.1	XP_014263945.1	serine/threonine-protein kinase WNK4 isoform X1
XM_014408460.2	XP_014263946.1	serine/threonine-protein kinase WNK4 isoform X2
XM_014408460.1	XP_014263946.1	serine/threonine-protein kinase WNK4 isoform X2
XM_014408460.3	XP_014263946.2	serine/threonine-protein kinase WNK4 isoform X2
XM_014408459.2	XP_014263945.1	serine/threonine-protein kinase WNK4 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101477194 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101477194 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa03641	XM_014408460.2 Latest version!	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa376847	XM_014408459.3 Latest version!	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa376848	XM_014408460.3 Latest version!	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa03640	XM_014408459.2 Latest version!	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMa03641	XM_014408460.1	Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa03640	XM_014408459.1	Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101477194 ( XM_014408460.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263946.1 Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa03641
Clone ID Related Accession (Same CDS sequence)	XM_014408460.2 , XM_014408460.1
Accession Version	XM_014408460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGGTTCTCC TCTTCATAAT 
CAGCACCATC ACCGCCCTAC ATCTCTCCTG CCTAATCACC AGCAATACCA CTTGCCCTTT 
ATGAACCACA CCCACTTTCC ATTCCCATAT CCAGGGTCTC CTGGTTCACC CAAGCCAGCC 
GGGATCCATC CTCCCCTCAC ACGTGTAGCC AGTAACCCCA CCTTCCCCTC TGTTCCCTCC 
CCAACCCCAA ACTCGCCGAG TCCTGTGAGT GAGACCCCAA GTCCTGTCAA CACGGAATTT 
GTTAACATAT CCCCTCCTGC CCTTCAAACT CCCACCATGT GGCCCCCCCA CTCGCAGCCC 
CTTTTTTCCT TAGCCAATGT AATTTCCATG GCAATGAGCA TGGCTCAGTC TTTTATCCCT 
CCTGCAGGCC TTCCCACCCA AGGGCTGCCC TCATATCCAA GCTTTCTCCC CCACCTACAC 
AGTCATATGG CTCCACAGCC AGGTTACCCC TCTGTCTACC CTCAACCTTA CCAGACTTCA 
CCAGTAGAGG CCTCTTACCC TGCAGGGGGG ATCCCAGTGC AGAACAATGT CTACTACAGC 
CCCGAGCATA TGCCTCAAAT GGATGGAGCT AATTTAACTC ACAGCAATCC TCCACAGTGG 
CCCCATCCTG CCCCCCCACC ATCCATGGCT TTCACTCCTC CTCCACCTCC TCCTGCACAG 
CAGGTTGGAC TCACCGGTTC CCCCAGCCGG ACCCAGGAAA GGAGTCACAT GCCCCCAACC 
TCAGCCCAAG CTCCAGCTCC AGCTAAACCC AGACCAGAGG TCTCCAGTTC GAATTCAAGT 
TCAAGCTCAT CAGATCTTTC ACCATCACCC CCAGAGAGCC CAGAAAACAC TTTATCATCT 
TTCAACAGCC GGTTGCACCC TGCTGTGCCA GCGAAGAATA CAAGTACCCC CAAACCAAAA 
GCTTCTTCCC CCAAATCTGA AGTTCAGTCT GCATCAGTGA CTGTTGGACG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCC TTTGCCTGAT GCGCCTGCAC CTGCAGCCCC CAAGCCCGCA 
GAGAACGGCA GCAGCCAGTC GGAGAGCAGC ACAGAGGAGC AGGGTGAGTC TGAGGCGAGC 
CTGGCCACCT CCTTCACAAT GACCCCTCCT CACCCACAAC CCCGCAAAGG TTCTTCGGGA 
GTCCAACAGG AGGCTGACGG GCAGGCTGGA TGGGCAGTGA GCCAGGAGCA GCAGCAGCAG 
GAAGAGGAAG ATGAAGATGA GGAAGAGGTT ATCGGGGAAC AGCCAAGGGG GAGGAGGAGA 
AGCCGTCGAA GGGCATACAG CCTGAGCCTG TTGGGAACGT CTGCAGACAG TGGGCTCTCT 
GTGACGGCTG CTGATACAGA GGGGAGGCTG TGGGACGGAG CAGCGGACAG TCCACAGTAC 
ACCAATGCCC TTCACAATTT ATGGATGATG ACTTATAACC GCAGTGCTCC CTACCTAAGC 
AGTGATGACT CAGACAGTGA AGATGAGGAC ATGCTGGAAG AGCTTCAGGA GCTCAGAGAG 
AAACATCTGG CAGAAGTTCT GGCCCTGCAG GCTGCCCAGA AAAGAGAAAT TGAAGAACTA 
TATAAGAAGA TGGGAAAGGT CCCCCCTCCG GGCATCGTCT CTCCTGCTGC TATGTTGTCC 
AGCCGACAGC GCCGCCTCTC CAAAGGAAAC GGTTTTCCTT CGTCTCGCAG GAACAGCCTT 
CAGCGACTGG AGATGTTACC GCTTCAAGGT ATCATTAGGA GAAACTCTCT TGGAGGCAGC 
AGCAGTGGCT CGCAGGACAA ACCTTTTAAA GGCGTCACTT TTGCCACAGA CCTAAGTAGA 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263946.1
CDS	230..4615
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPPLTPLTPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNISPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMAFTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408460.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408460.2

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGGTTCTCC TCTTCATAAT 
CAGCACCATC ACCGCCCTAC ATCTCTCCTG CCTAATCACC AGCAATACCA CTTGCCCTTT 
ATGAACCACA CCCACTTTCC ATTCCCATAT CCAGGGTCTC CTGGTTCACC CAAGCCAGCC 
GGGATCCATC CTCCCCTCAC ACGTGTAGCC AGTAACCCCA CCTTCCCCTC TGTTCCCTCC 
CCAACCCCAA ACTCGCCGAG TCCTGTGAGT GAGACCCCAA GTCCTGTCAA CACGGAATTT 
GTTAACATAT CCCCTCCTGC CCTTCAAACT CCCACCATGT GGCCCCCCCA CTCGCAGCCC 
CTTTTTTCCT TAGCCAATGT AATTTCCATG GCAATGAGCA TGGCTCAGTC TTTTATCCCT 
CCTGCAGGCC TTCCCACCCA AGGGCTGCCC TCATATCCAA GCTTTCTCCC CCACCTACAC 
AGTCATATGG CTCCACAGCC AGGTTACCCC TCTGTCTACC CTCAACCTTA CCAGACTTCA 
CCAGTAGAGG CCTCTTACCC TGCAGGGGGG ATCCCAGTGC AGAACAATGT CTACTACAGC 
CCCGAGCATA TGCCTCAAAT GGATGGAGCT AATTTAACTC ACAGCAATCC TCCACAGTGG 
CCCCATCCTG CCCCCCCACC ATCCATGGCT TTCACTCCTC CTCCACCTCC TCCTGCACAG 
CAGGTTGGAC TCACCGGTTC CCCCAGCCGG ACCCAGGAAA GGAGTCACAT GCCCCCAACC 
TCAGCCCAAG CTCCAGCTCC AGCTAAACCC AGACCAGAGG TCTCCAGTTC GAATTCAAGT 
TCAAGCTCAT CAGATCTTTC ACCATCACCC CCAGAGAGCC CAGAAAACAC TTTATCATCT 
TTCAACAGCC GGTTGCACCC TGCTGTGCCA GCGAAGAATA CAAGTACCCC CAAACCAAAA 
GCTTCTTCCC CCAAATCTGA AGTTCAGTCT GCATCAGTGA CTGTTGGACG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCC TTTGCCTGAT GCGCCTGCAC CTGCAGCCCC CAAGCCCGCA 
GAGAACGGCA GCAGCCAGTC GGAGAGCAGC ACAGAGGAGC AGGGTGAGTC TGAGGCGAGC 
CTGGCCACCT CCTTCACAAT GACCCCTCCT CACCCACAAC CCCGCAAAGG TTCTTCGGGA 
GTCCAACAGG AGGCTGACGG GCAGGCTGGA TGGGCAGTGA GCCAGGAGCA GCAGCAGCAG 
GAAGAGGAAG ATGAAGATGA GGAAGAGGTT ATCGGGGAAC AGCCAAGGGG GAGGAGGAGA 
AGCCGTCGAA GGGCATACAG CCTGAGCCTG TTGGGAACGT CTGCAGACAG TGGGCTCTCT 
GTGACGGCTG CTGATACAGA GGGGAGGCTG TGGGACGGAG CAGCGGACAG TCCACAGTAC 
ACCAATGCCC TTCACAATTT ATGGATGATG ACTTATAACC GCAGTGCTCC CTACCTAAGC 
AGTGATGACT CAGACAGTGA AGATGAGGAC ATGCTGGAAG AGCTTCAGGA GCTCAGAGAG 
AAACATCTGG CAGAAGTTCT GGCCCTGCAG GCTGCCCAGA AAAGAGAAAT TGAAGAACTA 
TATAAGAAGA TGGGAAAGGT CCCCCCTCCG GGCATCGTCT CTCCTGCTGC TATGTTGTCC 
AGCCGACAGC GCCGCCTCTC CAAAGGAAAC GGTTTTCCTT CGTCTCGCAG GAACAGCCTT 
CAGCGACTGG AGATGTTACC GCTTCAAGGT ATCATTAGGA GAAACTCTCT TGGAGGCAGC 
AGCAGTGGCT CGCAGGACAA ACCTTTTAAA GGCGTCACTT TTGCCACAGA CCTAAGTAGA 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477194 ( XM_014408460.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263946.1 Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa03641
Clone ID Related Accession (Same CDS sequence)	XM_014408460.2 , XM_014408460.1
Accession Version	XM_014408460.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGGTTCTCC TCTTCATAAT 
CAGCACCATC ACCGCCCTAC ATCTCTCCTG CCTAATCACC AGCAATACCA CTTGCCCTTT 
ATGAACCACA CCCACTTTCC ATTCCCATAT CCAGGGTCTC CTGGTTCACC CAAGCCAGCC 
GGGATCCATC CTCCCCTCAC ACGTGTAGCC AGTAACCCCA CCTTCCCCTC TGTTCCCTCC 
CCAACCCCAA ACTCGCCGAG TCCTGTGAGT GAGACCCCAA GTCCTGTCAA CACGGAATTT 
GTTAACATAT CCCCTCCTGC CCTTCAAACT CCCACCATGT GGCCCCCCCA CTCGCAGCCC 
CTTTTTTCCT TAGCCAATGT AATTTCCATG GCAATGAGCA TGGCTCAGTC TTTTATCCCT 
CCTGCAGGCC TTCCCACCCA AGGGCTGCCC TCATATCCAA GCTTTCTCCC CCACCTACAC 
AGTCATATGG CTCCACAGCC AGGTTACCCC TCTGTCTACC CTCAACCTTA CCAGACTTCA 
CCAGTAGAGG CCTCTTACCC TGCAGGGGGG ATCCCAGTGC AGAACAATGT CTACTACAGC 
CCCGAGCATA TGCCTCAAAT GGATGGAGCT AATTTAACTC ACAGCAATCC TCCACAGTGG 
CCCCATCCTG CCCCCCCACC ATCCATGGCT TTCACTCCTC CTCCACCTCC TCCTGCACAG 
CAGGTTGGAC TCACCGGTTC CCCCAGCCGG ACCCAGGAAA GGAGTCACAT GCCCCCAACC 
TCAGCCCAAG CTCCAGCTCC AGCTAAACCC AGACCAGAGG TCTCCAGTTC GAATTCAAGT 
TCAAGCTCAT CAGATCTTTC ACCATCACCC CCAGAGAGCC CAGAAAACAC TTTATCATCT 
TTCAACAGCC GGTTGCACCC TGCTGTGCCA GCGAAGAATA CAAGTACCCC CAAACCAAAA 
GCTTCTTCCC CCAAATCTGA AGTTCAGTCT GCATCAGTGA CTGTTGGACG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCC TTTGCCTGAT GCGCCTGCAC CTGCAGCCCC CAAGCCCGCA 
GAGAACGGCA GCAGCCAGTC GGAGAGCAGC ACAGAGGAGC AGGGTGAGTC TGAGGCGAGC 
CTGGCCACCT CCTTCACAAT GACCCCTCCT CACCCACAAC CCCGCAAAGG TTCTTCGGGA 
GTCCAACAGG AGGCTGACGG GCAGGCTGGA TGGGCAGTGA GCCAGGAGCA GCAGCAGCAG 
GAAGAGGAAG ATGAAGATGA GGAAGAGGTT ATCGGGGAAC AGCCAAGGGG GAGGAGGAGA 
AGCCGTCGAA GGGCATACAG CCTGAGCCTG TTGGGAACGT CTGCAGACAG TGGGCTCTCT 
GTGACGGCTG CTGATACAGA GGGGAGGCTG TGGGACGGAG CAGCGGACAG TCCACAGTAC 
ACCAATGCCC TTCACAATTT ATGGATGATG ACTTATAACC GCAGTGCTCC CTACCTAAGC 
AGTGATGACT CAGACAGTGA AGATGAGGAC ATGCTGGAAG AGCTTCAGGA GCTCAGAGAG 
AAACATCTGG CAGAAGTTCT GGCCCTGCAG GCTGCCCAGA AAAGAGAAAT TGAAGAACTA 
TATAAGAAGA TGGGAAAGGT CCCCCCTCCG GGCATCGTCT CTCCTGCTGC TATGTTGTCC 
AGCCGACAGC GCCGCCTCTC CAAAGGAAAC GGTTTTCCTT CGTCTCGCAG GAACAGCCTT 
CAGCGACTGG AGATGTTACC GCTTCAAGGT ATCATTAGGA GAAACTCTCT TGGAGGCAGC 
AGCAGTGGCT CGCAGGACAA ACCTTTTAAA GGCGTCACTT TTGCCACAGA CCTAAGTAGA 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263946.1
CDS	223..4608
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPPLTPLTPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNISPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMAFTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408460.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408460.1

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGGTTCTCC TCTTCATAAT 
CAGCACCATC ACCGCCCTAC ATCTCTCCTG CCTAATCACC AGCAATACCA CTTGCCCTTT 
ATGAACCACA CCCACTTTCC ATTCCCATAT CCAGGGTCTC CTGGTTCACC CAAGCCAGCC 
GGGATCCATC CTCCCCTCAC ACGTGTAGCC AGTAACCCCA CCTTCCCCTC TGTTCCCTCC 
CCAACCCCAA ACTCGCCGAG TCCTGTGAGT GAGACCCCAA GTCCTGTCAA CACGGAATTT 
GTTAACATAT CCCCTCCTGC CCTTCAAACT CCCACCATGT GGCCCCCCCA CTCGCAGCCC 
CTTTTTTCCT TAGCCAATGT AATTTCCATG GCAATGAGCA TGGCTCAGTC TTTTATCCCT 
CCTGCAGGCC TTCCCACCCA AGGGCTGCCC TCATATCCAA GCTTTCTCCC CCACCTACAC 
AGTCATATGG CTCCACAGCC AGGTTACCCC TCTGTCTACC CTCAACCTTA CCAGACTTCA 
CCAGTAGAGG CCTCTTACCC TGCAGGGGGG ATCCCAGTGC AGAACAATGT CTACTACAGC 
CCCGAGCATA TGCCTCAAAT GGATGGAGCT AATTTAACTC ACAGCAATCC TCCACAGTGG 
CCCCATCCTG CCCCCCCACC ATCCATGGCT TTCACTCCTC CTCCACCTCC TCCTGCACAG 
CAGGTTGGAC TCACCGGTTC CCCCAGCCGG ACCCAGGAAA GGAGTCACAT GCCCCCAACC 
TCAGCCCAAG CTCCAGCTCC AGCTAAACCC AGACCAGAGG TCTCCAGTTC GAATTCAAGT 
TCAAGCTCAT CAGATCTTTC ACCATCACCC CCAGAGAGCC CAGAAAACAC TTTATCATCT 
TTCAACAGCC GGTTGCACCC TGCTGTGCCA GCGAAGAATA CAAGTACCCC CAAACCAAAA 
GCTTCTTCCC CCAAATCTGA AGTTCAGTCT GCATCAGTGA CTGTTGGACG ATTCCAGGTG 
ACGCCCAGCA TGGACATCCC TTTGCCTGAT GCGCCTGCAC CTGCAGCCCC CAAGCCCGCA 
GAGAACGGCA GCAGCCAGTC GGAGAGCAGC ACAGAGGAGC AGGGTGAGTC TGAGGCGAGC 
CTGGCCACCT CCTTCACAAT GACCCCTCCT CACCCACAAC CCCGCAAAGG TTCTTCGGGA 
GTCCAACAGG AGGCTGACGG GCAGGCTGGA TGGGCAGTGA GCCAGGAGCA GCAGCAGCAG 
GAAGAGGAAG ATGAAGATGA GGAAGAGGTT ATCGGGGAAC AGCCAAGGGG GAGGAGGAGA 
AGCCGTCGAA GGGCATACAG CCTGAGCCTG TTGGGAACGT CTGCAGACAG TGGGCTCTCT 
GTGACGGCTG CTGATACAGA GGGGAGGCTG TGGGACGGAG CAGCGGACAG TCCACAGTAC 
ACCAATGCCC TTCACAATTT ATGGATGATG ACTTATAACC GCAGTGCTCC CTACCTAAGC 
AGTGATGACT CAGACAGTGA AGATGAGGAC ATGCTGGAAG AGCTTCAGGA GCTCAGAGAG 
AAACATCTGG CAGAAGTTCT GGCCCTGCAG GCTGCCCAGA AAAGAGAAAT TGAAGAACTA 
TATAAGAAGA TGGGAAAGGT CCCCCCTCCG GGCATCGTCT CTCCTGCTGC TATGTTGTCC 
AGCCGACAGC GCCGCCTCTC CAAAGGAAAC GGTTTTCCTT CGTCTCGCAG GAACAGCCTT 
CAGCGACTGG AGATGTTACC GCTTCAAGGT ATCATTAGGA GAAACTCTCT TGGAGGCAGC 
AGCAGTGGCT CGCAGGACAA ACCTTTTAAA GGCGTCACTT TTGCCACAGA CCTAAGTAGA 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477194 ( XM_014408459.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263945.2 Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.

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CloneID	OMa376847
Clone ID Related Accession (Same CDS sequence)	XM_014408459.3
Accession Version	XM_014408459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036787.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CTTTGACGCC ATTGACACCA TTGGCCCCAG TTTTGTCCCC CGTCACCAGC 
TCTGTGGACT CTGGCGTCAG CACCCACTAC CCTGCAGAGC CAGAGGAGCC CGAAGTTGAC 
CAGCACTTTC AAGTTCGCCA CAACAGCCTG TCTTCTGCTA ACTCTGACTG CGAGACGGAC 
GGCTATCTGA GCCCCTCTGG TCTTCAGGAT CCGCTGGAGG CCGGCAACGT CGGCATGCCT 
GCGAACTCTC CCACCACACA TCCCAACCCC CCCGCCGTAA ACGGTATCTC CCTCCGCTTC 
CCCTCGAGTA TTGCTGTCTC CAACAACATA GAACACGGCA GTTCAAGGCC TACGAGTGGC 
TTCAACTCCC CCGTGGAGAG CTACGCGTCT GATGTGACTT CAAGCCTGAG CGACGGCTAC 
GAGGGCCTAT CGGAGAAGAA CGAGAAATCG GTGGCGAGAC GAACGGCTGC GAAGCTGTTC 
AGGAGGAGGG CGCGCTCGAG GCTTCGCATC ATCGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAATGAC AAGATGGTGA CCTTCAAGTT CGACCTGGAT 
GGAGATAACC CAGAAGACAT TGCTGCTGTC ATGGTGCACA ACGAGTTCAT CTTGCCATCA 
GAGAAGGAGG GCTTTATCTA CCGCTTGGGT GACATCATCA AACGAGCCGA GGCACTGTTT 
GCCAAACAAC AGCCGGTCCA CTCCACTGGG CACCGACTTG CCCAGCCTGC ATTGCACGGA 
GGGATTAGCT CACTGTCGTC CTCACAGCCT AATCTGCACA ATCACGCCCT GCCTCGAACA 
CACTCGTCCT CTTCTCTGCC TGACTGCAGT GTGGCTAAAC CGAGTGCAGG GGTACGTGGC 
TTGCCCCCAT GTCCCAATGG AGAGGTCTCC AGTGCAGACA TTACTTCACC TGTGCGACCT 
CTAATACGCT CACAGTCTTT CCACAACGCC CCAGGTTCTC CTCTTCATAA TCAGCACCAT 
CACCGCCCTA CATCTCTCCT GCCTAATCAC CAGCAATACC ACTTGCCCTT TATGAACCAC 
ACCCACTTTC CATTCCCATA TCCAGGGTCT CCTGGTTCAC CCAAGCCAGC CGGGATCCAT 
CCTCCCCTCA CACGTGTAGC CAGTAACCCC ACCTTCCCCT CTGTTCCCTC CCCAACCCCA 
AACTCGCCGA GTCCTGTGAG TGAGACCCCA AGTCCTGTCA ACACGGAATT TGTTAACATA 
CCCCCTCCTG CCCTTCAAAC TCCCACCATG TGGCCCCCCC ACTCGCAGCC CCTTTTTTCC 
TTAGCCAATG TAATTTCCAT GGCAATGAGC ATGGCTCAGT CTTTTATCCC TCCTGCAGGC 
CTTCCCACCC AAGGGCTGCC CTCATATCCA AGCTTTCTCC CCCACCTACA CAGTCATATG 
GCTCCACAGC CAGGTTACCC CTCTGTCTAC CCTCAACCTT ACCAGACTTC ACCAGTAGAG 
GCCTCTTACC CTGCAGGGGG GATCCCAGTG CAGAACAATG TCTACTACAG CCCCGAGCAT 
ATGCCTCAAA TGGATGGAGC TAATTTAACT CACAGCAATC CTCCACAGTG GCCCCATCCT 
GCCCCCCCAC CATCCATGGC TTCCACTCCT CCTCCACCTC CTCCTGCACA GCAGGTTGGA 
CTCACCGGTT CCCCCAGCCG GACCCAGGAA AGGAGTCACA TGCCCCCAAC CTCAGCCCAA 
GCTCCAGCTC CAGCTAAACC CAGACCAGAG GTCTCCAGTT CGAATTCAAG TTCAAGCTCA 
TCAGATCTTT CACCATCACC CCCAGAGAGC CCAGAAAACA CTTTATCATC TTTCAACAGC 
CGGTTGCACC CTGCTGTGCC AGCGAAGAAT ACAAGTACCC CCAAACCAAA AGCTTCTTCC 
CCCAAATCTG AAGTTCAGTC TGCATCAGTG ACTGTTGGAC GATTCCAGGT GACGCCCAGC 
ATGGACATCC CTTTGCCTGA TGCGCCTGCA CCTGCAGCCC CCAAGCCCGC AGAGAACGGC 
AGCAGCCAGT CGGAGAGCAG CACAGAGGAG CAGGGTGAGT CTGAGGCGAG CCTGGCCACC 
TCCTTCACAA TGACCCCTCC TCACCCACAA CCCCGCAAAG GTTCTTCGGG AGTCCAACAG 
GAGGCTGACG GGCAGGCTGG ATGGGCAGTG AGCCAGGAGC AGCAGCAGCA GGAAGAGGAA 
GATGAAGATG AGGAAGAGGT TATCGGGGAA CAGCCAAGGG GGAGGAGGAG AAGCCGTCGA 
AGGGCATACA GCCTGAGCCT GTTGGGAACG TCTGCAGACA GTGGGCTCTC TGTGACGGCT 
GCTGATACAG AGGGGAGGCT GTGGGACGGA GCAGCGGACA GTCCACAGTA CACCAATGCC 
CTTCACAATT TATGGATGAT GACTTATAAC CGCAGTGCTC CCTACCTAAG CAGTGATGAC 
TCAGACAGTG AAGATGAGGA CATGCTGGAA GAGCTTCAGG AGCTCAGAGA GAAACATCTG 
GCAGAAGTTC TGGCCCTGCA GGCTGCCCAG AAAAGAGAAA TTGAAGAACT ATATAAGAAG 
ATGGGAAAGG TCCCCCCTCC GGGCATCGTC TCTCCTGCTG CTATGTTGTC CAGCCGACAG 
CGCCGCCTCT CCAAAGGAAA CGGTTTTCCT TCGTCTCGCA GGAACAGCCT TCAGCGACTG 
GAGATGTTAC CGCTTCAAGG TATCATTAGG AGAAACTCTC TTGGAGGCAG CAGCAGTGGC 
TCGCAGGACA AACCTTTTAA AGGCGTCACT TTTGCCACAG ACCTAAGTAG AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263945.2
CDS	229..4725
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPDCSVAKPSAGVRGLPPCPNGEVSSADITSPVRPLIRSQSFHNAPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNIPPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMASTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408459.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408459.3

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CTTTGACGCC ATTGACACCA TTGGCCCCAG TTTTGTCCCC CGTCACCAGC 
TCTGTGGACT CTGGCGTCAG CACCCACTAC CCTGCAGAGC CAGAGGAGCC CGAAGTTGAC 
CAGCACTTTC AAGTTCGCCA CAACAGCCTG TCTTCTGCTA ACTCTGACTG CGAGACGGAC 
GGCTATCTGA GCCCCTCTGG TCTTCAGGAT CCGCTGGAGG CCGGCAACGT CGGCATGCCT 
GCGAACTCTC CCACCACACA TCCCAACCCC CCCGCCGTAA ACGGTATCTC CCTCCGCTTC 
CCCTCGAGTA TTGCTGTCTC CAACAACATA GAACACGGCA GTTCAAGGCC TACGAGTGGC 
TTCAACTCCC CCGTGGAGAG CTACGCGTCT GATGTGACTT CAAGCCTGAG CGACGGCTAC 
GAGGGCCTAT CGGAGAAGAA CGAGAAATCG GTGGCGAGAC GAACGGCTGC GAAGCTGTTC 
AGGAGGAGGG CGCGCTCGAG GCTTCGCATC ATCGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAATGAC AAGATGGTGA CCTTCAAGTT CGACCTGGAT 
GGAGATAACC CAGAAGACAT TGCTGCTGTC ATGGTGCACA ACGAGTTCAT CTTGCCATCA 
GAGAAGGAGG GCTTTATCTA CCGCTTGGGT GACATCATCA AACGAGCCGA GGCACTGTTT 
GCCAAACAAC AGCCGGTCCA CTCCACTGGG CACCGACTTG CCCAGCCTGC ATTGCACGGA 
GGGATTAGCT CACTGTCGTC CTCACAGCCT AATCTGCACA ATCACGCCCT GCCTCGAACA 
CACTCGTCCT CTTCTCTGCC TGACTGCAGT GTGGCTAAAC CGAGTGCAGG GGTACGTGGC 
TTGCCCCCAT GTCCCAATGG AGAGGTCTCC AGTGCAGACA TTACTTCACC TGTGCGACCT 
CTAATACGCT CACAGTCTTT CCACAACGCC CCAGGTTCTC CTCTTCATAA TCAGCACCAT 
CACCGCCCTA CATCTCTCCT GCCTAATCAC CAGCAATACC ACTTGCCCTT TATGAACCAC 
ACCCACTTTC CATTCCCATA TCCAGGGTCT CCTGGTTCAC CCAAGCCAGC CGGGATCCAT 
CCTCCCCTCA CACGTGTAGC CAGTAACCCC ACCTTCCCCT CTGTTCCCTC CCCAACCCCA 
AACTCGCCGA GTCCTGTGAG TGAGACCCCA AGTCCTGTCA ACACGGAATT TGTTAACATA 
CCCCCTCCTG CCCTTCAAAC TCCCACCATG TGGCCCCCCC ACTCGCAGCC CCTTTTTTCC 
TTAGCCAATG TAATTTCCAT GGCAATGAGC ATGGCTCAGT CTTTTATCCC TCCTGCAGGC 
CTTCCCACCC AAGGGCTGCC CTCATATCCA AGCTTTCTCC CCCACCTACA CAGTCATATG 
GCTCCACAGC CAGGTTACCC CTCTGTCTAC CCTCAACCTT ACCAGACTTC ACCAGTAGAG 
GCCTCTTACC CTGCAGGGGG GATCCCAGTG CAGAACAATG TCTACTACAG CCCCGAGCAT 
ATGCCTCAAA TGGATGGAGC TAATTTAACT CACAGCAATC CTCCACAGTG GCCCCATCCT 
GCCCCCCCAC CATCCATGGC TTCCACTCCT CCTCCACCTC CTCCTGCACA GCAGGTTGGA 
CTCACCGGTT CCCCCAGCCG GACCCAGGAA AGGAGTCACA TGCCCCCAAC CTCAGCCCAA 
GCTCCAGCTC CAGCTAAACC CAGACCAGAG GTCTCCAGTT CGAATTCAAG TTCAAGCTCA 
TCAGATCTTT CACCATCACC CCCAGAGAGC CCAGAAAACA CTTTATCATC TTTCAACAGC 
CGGTTGCACC CTGCTGTGCC AGCGAAGAAT ACAAGTACCC CCAAACCAAA AGCTTCTTCC 
CCCAAATCTG AAGTTCAGTC TGCATCAGTG ACTGTTGGAC GATTCCAGGT GACGCCCAGC 
ATGGACATCC CTTTGCCTGA TGCGCCTGCA CCTGCAGCCC CCAAGCCCGC AGAGAACGGC 
AGCAGCCAGT CGGAGAGCAG CACAGAGGAG CAGGGTGAGT CTGAGGCGAG CCTGGCCACC 
TCCTTCACAA TGACCCCTCC TCACCCACAA CCCCGCAAAG GTTCTTCGGG AGTCCAACAG 
GAGGCTGACG GGCAGGCTGG ATGGGCAGTG AGCCAGGAGC AGCAGCAGCA GGAAGAGGAA 
GATGAAGATG AGGAAGAGGT TATCGGGGAA CAGCCAAGGG GGAGGAGGAG AAGCCGTCGA 
AGGGCATACA GCCTGAGCCT GTTGGGAACG TCTGCAGACA GTGGGCTCTC TGTGACGGCT 
GCTGATACAG AGGGGAGGCT GTGGGACGGA GCAGCGGACA GTCCACAGTA CACCAATGCC 
CTTCACAATT TATGGATGAT GACTTATAAC CGCAGTGCTC CCTACCTAAG CAGTGATGAC 
TCAGACAGTG AAGATGAGGA CATGCTGGAA GAGCTTCAGG AGCTCAGAGA GAAACATCTG 
GCAGAAGTTC TGGCCCTGCA GGCTGCCCAG AAAAGAGAAA TTGAAGAACT ATATAAGAAG 
ATGGGAAAGG TCCCCCCTCC GGGCATCGTC TCTCCTGCTG CTATGTTGTC CAGCCGACAG 
CGCCGCCTCT CCAAAGGAAA CGGTTTTCCT TCGTCTCGCA GGAACAGCCT TCAGCGACTG 
GAGATGTTAC CGCTTCAAGG TATCATTAGG AGAAACTCTC TTGGAGGCAG CAGCAGTGGC 
TCGCAGGACA AACCTTTTAA AGGCGTCACT TTTGCCACAG ACCTAAGTAG AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477194 ( XM_014408460.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263946.2 Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.

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CloneID	OMa376848
Clone ID Related Accession (Same CDS sequence)	XM_014408460.3
Accession Version	XM_014408460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036787.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CTTTGACGCC ATTGACACCA TTGGCCCCAG TTTTGTCCCC CGTCACCAGC 
TCTGTGGACT CTGGCGTCAG CACCCACTAC CCTGCAGAGC CAGAGGAGCC CGAAGTTGAC 
CAGCACTTTC AAGTTCGCCA CAACAGCCTG TCTTCTGCTA ACTCTGACTG CGAGACGGAC 
GGCTATCTGA GCCCCTCTGG TCTTCAGGAT CCGCTGGAGG CCGGCAACGT CGGCATGCCT 
GCGAACTCTC CCACCACACA TCCCAACCCC CCCGCCGTAA ACGGTATCTC CCTCCGCTTC 
CCCTCGAGTA TTGCTGTCTC CAACAACATA GAACACGGCA GTTCAAGGCC TACGAGTGGC 
TTCAACTCCC CCGTGGAGAG CTACGCGTCT GATGTGACTT CAAGCCTGAG CGACGGCTAC 
GAGGGCCTAT CGGAGAAGAA CGAGAAATCG GTGGCGAGAC GAACGGCTGC GAAGCTGTTC 
AGGAGGAGGG CGCGCTCGAG GCTTCGCATC ATCGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAATGAC AAGATGGTGA CCTTCAAGTT CGACCTGGAT 
GGAGATAACC CAGAAGACAT TGCTGCTGTC ATGGTGCACA ACGAGTTCAT CTTGCCATCA 
GAGAAGGAGG GCTTTATCTA CCGCTTGGGT GACATCATCA AACGAGCCGA GGCACTGTTT 
GCCAAACAAC AGCCGGTCCA CTCCACTGGG CACCGACTTG CCCAGCCTGC ATTGCACGGA 
GGGATTAGCT CACTGTCGTC CTCACAGCCT AATCTGCACA ATCACGCCCT GCCTCGAACA 
CACTCGTCCT CTTCTCTGCC TGGTTCTCCT CTTCATAATC AGCACCATCA CCGCCCTACA 
TCTCTCCTGC CTAATCACCA GCAATACCAC TTGCCCTTTA TGAACCACAC CCACTTTCCA 
TTCCCATATC CAGGGTCTCC TGGTTCACCC AAGCCAGCCG GGATCCATCC TCCCCTCACA 
CGTGTAGCCA GTAACCCCAC CTTCCCCTCT GTTCCCTCCC CAACCCCAAA CTCGCCGAGT 
CCTGTGAGTG AGACCCCAAG TCCTGTCAAC ACGGAATTTG TTAACATACC CCCTCCTGCC 
CTTCAAACTC CCACCATGTG GCCCCCCCAC TCGCAGCCCC TTTTTTCCTT AGCCAATGTA 
ATTTCCATGG CAATGAGCAT GGCTCAGTCT TTTATCCCTC CTGCAGGCCT TCCCACCCAA 
GGGCTGCCCT CATATCCAAG CTTTCTCCCC CACCTACACA GTCATATGGC TCCACAGCCA 
GGTTACCCCT CTGTCTACCC TCAACCTTAC CAGACTTCAC CAGTAGAGGC CTCTTACCCT 
GCAGGGGGGA TCCCAGTGCA GAACAATGTC TACTACAGCC CCGAGCATAT GCCTCAAATG 
GATGGAGCTA ATTTAACTCA CAGCAATCCT CCACAGTGGC CCCATCCTGC CCCCCCACCA 
TCCATGGCTT CCACTCCTCC TCCACCTCCT CCTGCACAGC AGGTTGGACT CACCGGTTCC 
CCCAGCCGGA CCCAGGAAAG GAGTCACATG CCCCCAACCT CAGCCCAAGC TCCAGCTCCA 
GCTAAACCCA GACCAGAGGT CTCCAGTTCG AATTCAAGTT CAAGCTCATC AGATCTTTCA 
CCATCACCCC CAGAGAGCCC AGAAAACACT TTATCATCTT TCAACAGCCG GTTGCACCCT 
GCTGTGCCAG CGAAGAATAC AAGTACCCCC AAACCAAAAG CTTCTTCCCC CAAATCTGAA 
GTTCAGTCTG CATCAGTGAC TGTTGGACGA TTCCAGGTGA CGCCCAGCAT GGACATCCCT 
TTGCCTGATG CGCCTGCACC TGCAGCCCCC AAGCCCGCAG AGAACGGCAG CAGCCAGTCG 
GAGAGCAGCA CAGAGGAGCA GGGTGAGTCT GAGGCGAGCC TGGCCACCTC CTTCACAATG 
ACCCCTCCTC ACCCACAACC CCGCAAAGGT TCTTCGGGAG TCCAACAGGA GGCTGACGGG 
CAGGCTGGAT GGGCAGTGAG CCAGGAGCAG CAGCAGCAGG AAGAGGAAGA TGAAGATGAG 
GAAGAGGTTA TCGGGGAACA GCCAAGGGGG AGGAGGAGAA GCCGTCGAAG GGCATACAGC 
CTGAGCCTGT TGGGAACGTC TGCAGACAGT GGGCTCTCTG TGACGGCTGC TGATACAGAG 
GGGAGGCTGT GGGACGGAGC AGCGGACAGT CCACAGTACA CCAATGCCCT TCACAATTTA 
TGGATGATGA CTTATAACCG CAGTGCTCCC TACCTAAGCA GTGATGACTC AGACAGTGAA 
GATGAGGACA TGCTGGAAGA GCTTCAGGAG CTCAGAGAGA AACATCTGGC AGAAGTTCTG 
GCCCTGCAGG CTGCCCAGAA AAGAGAAATT GAAGAACTAT ATAAGAAGAT GGGAAAGGTC 
CCCCCTCCGG GCATCGTCTC TCCTGCTGCT ATGTTGTCCA GCCGACAGCG CCGCCTCTCC 
AAAGGAAACG GTTTTCCTTC GTCTCGCAGG AACAGCCTTC AGCGACTGGA GATGTTACCG 
CTTCAAGGTA TCATTAGGAG AAACTCTCTT GGAGGCAGCA GCAGTGGCTC GCAGGACAAA 
CCTTTTAAAG GCGTCACTTT TGCCACAGAC CTAAGTAGAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263946.2
CDS	229..4593
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNIPPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMASTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408460.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408460.3

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CTTTGACGCC ATTGACACCA TTGGCCCCAG TTTTGTCCCC CGTCACCAGC 
TCTGTGGACT CTGGCGTCAG CACCCACTAC CCTGCAGAGC CAGAGGAGCC CGAAGTTGAC 
CAGCACTTTC AAGTTCGCCA CAACAGCCTG TCTTCTGCTA ACTCTGACTG CGAGACGGAC 
GGCTATCTGA GCCCCTCTGG TCTTCAGGAT CCGCTGGAGG CCGGCAACGT CGGCATGCCT 
GCGAACTCTC CCACCACACA TCCCAACCCC CCCGCCGTAA ACGGTATCTC CCTCCGCTTC 
CCCTCGAGTA TTGCTGTCTC CAACAACATA GAACACGGCA GTTCAAGGCC TACGAGTGGC 
TTCAACTCCC CCGTGGAGAG CTACGCGTCT GATGTGACTT CAAGCCTGAG CGACGGCTAC 
GAGGGCCTAT CGGAGAAGAA CGAGAAATCG GTGGCGAGAC GAACGGCTGC GAAGCTGTTC 
AGGAGGAGGG CGCGCTCGAG GCTTCGCATC ATCGGGCTCT CTGATAAAGT GGACCGGGTG 
GTGGAGTGCC AGCTGCAGAC GCACAATGAC AAGATGGTGA CCTTCAAGTT CGACCTGGAT 
GGAGATAACC CAGAAGACAT TGCTGCTGTC ATGGTGCACA ACGAGTTCAT CTTGCCATCA 
GAGAAGGAGG GCTTTATCTA CCGCTTGGGT GACATCATCA AACGAGCCGA GGCACTGTTT 
GCCAAACAAC AGCCGGTCCA CTCCACTGGG CACCGACTTG CCCAGCCTGC ATTGCACGGA 
GGGATTAGCT CACTGTCGTC CTCACAGCCT AATCTGCACA ATCACGCCCT GCCTCGAACA 
CACTCGTCCT CTTCTCTGCC TGGTTCTCCT CTTCATAATC AGCACCATCA CCGCCCTACA 
TCTCTCCTGC CTAATCACCA GCAATACCAC TTGCCCTTTA TGAACCACAC CCACTTTCCA 
TTCCCATATC CAGGGTCTCC TGGTTCACCC AAGCCAGCCG GGATCCATCC TCCCCTCACA 
CGTGTAGCCA GTAACCCCAC CTTCCCCTCT GTTCCCTCCC CAACCCCAAA CTCGCCGAGT 
CCTGTGAGTG AGACCCCAAG TCCTGTCAAC ACGGAATTTG TTAACATACC CCCTCCTGCC 
CTTCAAACTC CCACCATGTG GCCCCCCCAC TCGCAGCCCC TTTTTTCCTT AGCCAATGTA 
ATTTCCATGG CAATGAGCAT GGCTCAGTCT TTTATCCCTC CTGCAGGCCT TCCCACCCAA 
GGGCTGCCCT CATATCCAAG CTTTCTCCCC CACCTACACA GTCATATGGC TCCACAGCCA 
GGTTACCCCT CTGTCTACCC TCAACCTTAC CAGACTTCAC CAGTAGAGGC CTCTTACCCT 
GCAGGGGGGA TCCCAGTGCA GAACAATGTC TACTACAGCC CCGAGCATAT GCCTCAAATG 
GATGGAGCTA ATTTAACTCA CAGCAATCCT CCACAGTGGC CCCATCCTGC CCCCCCACCA 
TCCATGGCTT CCACTCCTCC TCCACCTCCT CCTGCACAGC AGGTTGGACT CACCGGTTCC 
CCCAGCCGGA CCCAGGAAAG GAGTCACATG CCCCCAACCT CAGCCCAAGC TCCAGCTCCA 
GCTAAACCCA GACCAGAGGT CTCCAGTTCG AATTCAAGTT CAAGCTCATC AGATCTTTCA 
CCATCACCCC CAGAGAGCCC AGAAAACACT TTATCATCTT TCAACAGCCG GTTGCACCCT 
GCTGTGCCAG CGAAGAATAC AAGTACCCCC AAACCAAAAG CTTCTTCCCC CAAATCTGAA 
GTTCAGTCTG CATCAGTGAC TGTTGGACGA TTCCAGGTGA CGCCCAGCAT GGACATCCCT 
TTGCCTGATG CGCCTGCACC TGCAGCCCCC AAGCCCGCAG AGAACGGCAG CAGCCAGTCG 
GAGAGCAGCA CAGAGGAGCA GGGTGAGTCT GAGGCGAGCC TGGCCACCTC CTTCACAATG 
ACCCCTCCTC ACCCACAACC CCGCAAAGGT TCTTCGGGAG TCCAACAGGA GGCTGACGGG 
CAGGCTGGAT GGGCAGTGAG CCAGGAGCAG CAGCAGCAGG AAGAGGAAGA TGAAGATGAG 
GAAGAGGTTA TCGGGGAACA GCCAAGGGGG AGGAGGAGAA GCCGTCGAAG GGCATACAGC 
CTGAGCCTGT TGGGAACGTC TGCAGACAGT GGGCTCTCTG TGACGGCTGC TGATACAGAG 
GGGAGGCTGT GGGACGGAGC AGCGGACAGT CCACAGTACA CCAATGCCCT TCACAATTTA 
TGGATGATGA CTTATAACCG CAGTGCTCCC TACCTAAGCA GTGATGACTC AGACAGTGAA 
GATGAGGACA TGCTGGAAGA GCTTCAGGAG CTCAGAGAGA AACATCTGGC AGAAGTTCTG 
GCCCTGCAGG CTGCCCAGAA AAGAGAAATT GAAGAACTAT ATAAGAAGAT GGGAAAGGTC 
CCCCCTCCGG GCATCGTCTC TCCTGCTGCT ATGTTGTCCA GCCGACAGCG CCGCCTCTCC 
AAAGGAAACG GTTTTCCTTC GTCTCGCAGG AACAGCCTTC AGCGACTGGA GATGTTACCG 
CTTCAAGGTA TCATTAGGAG AAACTCTCTT GGAGGCAGCA GCAGTGGCTC GCAGGACAAA 
CCTTTTAAAG GCGTCACTTT TGCCACAGAC CTAAGTAGAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477194 ( XM_014408459.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263945.1 Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa03640
Clone ID Related Accession (Same CDS sequence)	XM_014408459.1 , XM_014408459.2
Accession Version	XM_014408459.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGACTGCAG TGTGGCTAAA 
CCGAGTGCAG GGGTACGTGG CTTGCCCCCA TGTCCCAATG GAGAGGTCTC CAGTGCAGAC 
ATTACTTCAC CTGTGCGACC TCTAATACGC TCACAGTCTT TCCACAACGC CCCAGGTTCT 
CCTCTTCATA ATCAGCACCA TCACCGCCCT ACATCTCTCC TGCCTAATCA CCAGCAATAC 
CACTTGCCCT TTATGAACCA CACCCACTTT CCATTCCCAT ATCCAGGGTC TCCTGGTTCA 
CCCAAGCCAG CCGGGATCCA TCCTCCCCTC ACACGTGTAG CCAGTAACCC CACCTTCCCC 
TCTGTTCCCT CCCCAACCCC AAACTCGCCG AGTCCTGTGA GTGAGACCCC AAGTCCTGTC 
AACACGGAAT TTGTTAACAT ATCCCCTCCT GCCCTTCAAA CTCCCACCAT GTGGCCCCCC 
CACTCGCAGC CCCTTTTTTC CTTAGCCAAT GTAATTTCCA TGGCAATGAG CATGGCTCAG 
TCTTTTATCC CTCCTGCAGG CCTTCCCACC CAAGGGCTGC CCTCATATCC AAGCTTTCTC 
CCCCACCTAC ACAGTCATAT GGCTCCACAG CCAGGTTACC CCTCTGTCTA CCCTCAACCT 
TACCAGACTT CACCAGTAGA GGCCTCTTAC CCTGCAGGGG GGATCCCAGT GCAGAACAAT 
GTCTACTACA GCCCCGAGCA TATGCCTCAA ATGGATGGAG CTAATTTAAC TCACAGCAAT 
CCTCCACAGT GGCCCCATCC TGCCCCCCCA CCATCCATGG CTTTCACTCC TCCTCCACCT 
CCTCCTGCAC AGCAGGTTGG ACTCACCGGT TCCCCCAGCC GGACCCAGGA AAGGAGTCAC 
ATGCCCCCAA CCTCAGCCCA AGCTCCAGCT CCAGCTAAAC CCAGACCAGA GGTCTCCAGT 
TCGAATTCAA GTTCAAGCTC ATCAGATCTT TCACCATCAC CCCCAGAGAG CCCAGAAAAC 
ACTTTATCAT CTTTCAACAG CCGGTTGCAC CCTGCTGTGC CAGCGAAGAA TACAAGTACC 
CCCAAACCAA AAGCTTCTTC CCCCAAATCT GAAGTTCAGT CTGCATCAGT GACTGTTGGA 
CGATTCCAGG TGACGCCCAG CATGGACATC CCTTTGCCTG ATGCGCCTGC ACCTGCAGCC 
CCCAAGCCCG CAGAGAACGG CAGCAGCCAG TCGGAGAGCA GCACAGAGGA GCAGGGTGAG 
TCTGAGGCGA GCCTGGCCAC CTCCTTCACA ATGACCCCTC CTCACCCACA ACCCCGCAAA 
GGTTCTTCGG GAGTCCAACA GGAGGCTGAC GGGCAGGCTG GATGGGCAGT GAGCCAGGAG 
CAGCAGCAGC AGGAAGAGGA AGATGAAGAT GAGGAAGAGG TTATCGGGGA ACAGCCAAGG 
GGGAGGAGGA GAAGCCGTCG AAGGGCATAC AGCCTGAGCC TGTTGGGAAC GTCTGCAGAC 
AGTGGGCTCT CTGTGACGGC TGCTGATACA GAGGGGAGGC TGTGGGACGG AGCAGCGGAC 
AGTCCACAGT ACACCAATGC CCTTCACAAT TTATGGATGA TGACTTATAA CCGCAGTGCT 
CCCTACCTAA GCAGTGATGA CTCAGACAGT GAAGATGAGG ACATGCTGGA AGAGCTTCAG 
GAGCTCAGAG AGAAACATCT GGCAGAAGTT CTGGCCCTGC AGGCTGCCCA GAAAAGAGAA 
ATTGAAGAAC TATATAAGAA GATGGGAAAG GTCCCCCCTC CGGGCATCGT CTCTCCTGCT 
GCTATGTTGT CCAGCCGACA GCGCCGCCTC TCCAAAGGAA ACGGTTTTCC TTCGTCTCGC 
AGGAACAGCC TTCAGCGACT GGAGATGTTA CCGCTTCAAG GTATCATTAG GAGAAACTCT 
CTTGGAGGCA GCAGCAGTGG CTCGCAGGAC AAACCTTTTA AAGGCGTCAC TTTTGCCACA 
GACCTAAGTA GAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263945.1
CDS	223..4740
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPPLTPLTPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPDCSVAKPSAGVRGLPPCPNGEVSSADITSPVRPLIRSQSFHNAPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNISPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMAFTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408459.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra WNK lysine deficient protein kinase 4 (wnk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408459.1

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGACTGCAG TGTGGCTAAA 
CCGAGTGCAG GGGTACGTGG CTTGCCCCCA TGTCCCAATG GAGAGGTCTC CAGTGCAGAC 
ATTACTTCAC CTGTGCGACC TCTAATACGC TCACAGTCTT TCCACAACGC CCCAGGTTCT 
CCTCTTCATA ATCAGCACCA TCACCGCCCT ACATCTCTCC TGCCTAATCA CCAGCAATAC 
CACTTGCCCT TTATGAACCA CACCCACTTT CCATTCCCAT ATCCAGGGTC TCCTGGTTCA 
CCCAAGCCAG CCGGGATCCA TCCTCCCCTC ACACGTGTAG CCAGTAACCC CACCTTCCCC 
TCTGTTCCCT CCCCAACCCC AAACTCGCCG AGTCCTGTGA GTGAGACCCC AAGTCCTGTC 
AACACGGAAT TTGTTAACAT ATCCCCTCCT GCCCTTCAAA CTCCCACCAT GTGGCCCCCC 
CACTCGCAGC CCCTTTTTTC CTTAGCCAAT GTAATTTCCA TGGCAATGAG CATGGCTCAG 
TCTTTTATCC CTCCTGCAGG CCTTCCCACC CAAGGGCTGC CCTCATATCC AAGCTTTCTC 
CCCCACCTAC ACAGTCATAT GGCTCCACAG CCAGGTTACC CCTCTGTCTA CCCTCAACCT 
TACCAGACTT CACCAGTAGA GGCCTCTTAC CCTGCAGGGG GGATCCCAGT GCAGAACAAT 
GTCTACTACA GCCCCGAGCA TATGCCTCAA ATGGATGGAG CTAATTTAAC TCACAGCAAT 
CCTCCACAGT GGCCCCATCC TGCCCCCCCA CCATCCATGG CTTTCACTCC TCCTCCACCT 
CCTCCTGCAC AGCAGGTTGG ACTCACCGGT TCCCCCAGCC GGACCCAGGA AAGGAGTCAC 
ATGCCCCCAA CCTCAGCCCA AGCTCCAGCT CCAGCTAAAC CCAGACCAGA GGTCTCCAGT 
TCGAATTCAA GTTCAAGCTC ATCAGATCTT TCACCATCAC CCCCAGAGAG CCCAGAAAAC 
ACTTTATCAT CTTTCAACAG CCGGTTGCAC CCTGCTGTGC CAGCGAAGAA TACAAGTACC 
CCCAAACCAA AAGCTTCTTC CCCCAAATCT GAAGTTCAGT CTGCATCAGT GACTGTTGGA 
CGATTCCAGG TGACGCCCAG CATGGACATC CCTTTGCCTG ATGCGCCTGC ACCTGCAGCC 
CCCAAGCCCG CAGAGAACGG CAGCAGCCAG TCGGAGAGCA GCACAGAGGA GCAGGGTGAG 
TCTGAGGCGA GCCTGGCCAC CTCCTTCACA ATGACCCCTC CTCACCCACA ACCCCGCAAA 
GGTTCTTCGG GAGTCCAACA GGAGGCTGAC GGGCAGGCTG GATGGGCAGT GAGCCAGGAG 
CAGCAGCAGC AGGAAGAGGA AGATGAAGAT GAGGAAGAGG TTATCGGGGA ACAGCCAAGG 
GGGAGGAGGA GAAGCCGTCG AAGGGCATAC AGCCTGAGCC TGTTGGGAAC GTCTGCAGAC 
AGTGGGCTCT CTGTGACGGC TGCTGATACA GAGGGGAGGC TGTGGGACGG AGCAGCGGAC 
AGTCCACAGT ACACCAATGC CCTTCACAAT TTATGGATGA TGACTTATAA CCGCAGTGCT 
CCCTACCTAA GCAGTGATGA CTCAGACAGT GAAGATGAGG ACATGCTGGA AGAGCTTCAG 
GAGCTCAGAG AGAAACATCT GGCAGAAGTT CTGGCCCTGC AGGCTGCCCA GAAAAGAGAA 
ATTGAAGAAC TATATAAGAA GATGGGAAAG GTCCCCCCTC CGGGCATCGT CTCTCCTGCT 
GCTATGTTGT CCAGCCGACA GCGCCGCCTC TCCAAAGGAA ACGGTTTTCC TTCGTCTCGC 
AGGAACAGCC TTCAGCGACT GGAGATGTTA CCGCTTCAAG GTATCATTAG GAGAAACTCT 
CTTGGAGGCA GCAGCAGTGG CTCGCAGGAC AAACCTTTTA AAGGCGTCAC TTTTGCCACA 
GACCTAAGTA GAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101477194 ( XM_014408459.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263945.1 Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa03640
Clone ID Related Accession (Same CDS sequence)	XM_014408459.1 , XM_014408459.2
Accession Version	XM_014408459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444687.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGACTGCAG TGTGGCTAAA 
CCGAGTGCAG GGGTACGTGG CTTGCCCCCA TGTCCCAATG GAGAGGTCTC CAGTGCAGAC 
ATTACTTCAC CTGTGCGACC TCTAATACGC TCACAGTCTT TCCACAACGC CCCAGGTTCT 
CCTCTTCATA ATCAGCACCA TCACCGCCCT ACATCTCTCC TGCCTAATCA CCAGCAATAC 
CACTTGCCCT TTATGAACCA CACCCACTTT CCATTCCCAT ATCCAGGGTC TCCTGGTTCA 
CCCAAGCCAG CCGGGATCCA TCCTCCCCTC ACACGTGTAG CCAGTAACCC CACCTTCCCC 
TCTGTTCCCT CCCCAACCCC AAACTCGCCG AGTCCTGTGA GTGAGACCCC AAGTCCTGTC 
AACACGGAAT TTGTTAACAT ATCCCCTCCT GCCCTTCAAA CTCCCACCAT GTGGCCCCCC 
CACTCGCAGC CCCTTTTTTC CTTAGCCAAT GTAATTTCCA TGGCAATGAG CATGGCTCAG 
TCTTTTATCC CTCCTGCAGG CCTTCCCACC CAAGGGCTGC CCTCATATCC AAGCTTTCTC 
CCCCACCTAC ACAGTCATAT GGCTCCACAG CCAGGTTACC CCTCTGTCTA CCCTCAACCT 
TACCAGACTT CACCAGTAGA GGCCTCTTAC CCTGCAGGGG GGATCCCAGT GCAGAACAAT 
GTCTACTACA GCCCCGAGCA TATGCCTCAA ATGGATGGAG CTAATTTAAC TCACAGCAAT 
CCTCCACAGT GGCCCCATCC TGCCCCCCCA CCATCCATGG CTTTCACTCC TCCTCCACCT 
CCTCCTGCAC AGCAGGTTGG ACTCACCGGT TCCCCCAGCC GGACCCAGGA AAGGAGTCAC 
ATGCCCCCAA CCTCAGCCCA AGCTCCAGCT CCAGCTAAAC CCAGACCAGA GGTCTCCAGT 
TCGAATTCAA GTTCAAGCTC ATCAGATCTT TCACCATCAC CCCCAGAGAG CCCAGAAAAC 
ACTTTATCAT CTTTCAACAG CCGGTTGCAC CCTGCTGTGC CAGCGAAGAA TACAAGTACC 
CCCAAACCAA AAGCTTCTTC CCCCAAATCT GAAGTTCAGT CTGCATCAGT GACTGTTGGA 
CGATTCCAGG TGACGCCCAG CATGGACATC CCTTTGCCTG ATGCGCCTGC ACCTGCAGCC 
CCCAAGCCCG CAGAGAACGG CAGCAGCCAG TCGGAGAGCA GCACAGAGGA GCAGGGTGAG 
TCTGAGGCGA GCCTGGCCAC CTCCTTCACA ATGACCCCTC CTCACCCACA ACCCCGCAAA 
GGTTCTTCGG GAGTCCAACA GGAGGCTGAC GGGCAGGCTG GATGGGCAGT GAGCCAGGAG 
CAGCAGCAGC AGGAAGAGGA AGATGAAGAT GAGGAAGAGG TTATCGGGGA ACAGCCAAGG 
GGGAGGAGGA GAAGCCGTCG AAGGGCATAC AGCCTGAGCC TGTTGGGAAC GTCTGCAGAC 
AGTGGGCTCT CTGTGACGGC TGCTGATACA GAGGGGAGGC TGTGGGACGG AGCAGCGGAC 
AGTCCACAGT ACACCAATGC CCTTCACAAT TTATGGATGA TGACTTATAA CCGCAGTGCT 
CCCTACCTAA GCAGTGATGA CTCAGACAGT GAAGATGAGG ACATGCTGGA AGAGCTTCAG 
GAGCTCAGAG AGAAACATCT GGCAGAAGTT CTGGCCCTGC AGGCTGCCCA GAAAAGAGAA 
ATTGAAGAAC TATATAAGAA GATGGGAAAG GTCCCCCCTC CGGGCATCGT CTCTCCTGCT 
GCTATGTTGT CCAGCCGACA GCGCCGCCTC TCCAAAGGAA ACGGTTTTCC TTCGTCTCGC 
AGGAACAGCC TTCAGCGACT GGAGATGTTA CCGCTTCAAG GTATCATTAG GAGAAACTCT 
CTTGGAGGCA GCAGCAGTGG CTCGCAGGAC AAACCTTTTA AAGGCGTCAC TTTTGCCACA 
GACCTAAGTA GAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263945.1
CDS	230..4747
Translation	MSHAQVEIPGRGFPGLSVDMTDDCLSRVPSVLRTHGLGARRNSYGLQKISMDIDSPLYSGVLSKALSWSAENILSLPESRAEEEKTDDTPPSASPVSSSSTCRQDPDAGSVGGNWKPVARSTPQDPSSDSENELHNKQQKILSRITFDTQWEQEEKEDVETKAVATSPDGRYLKFNIEIGRGSFKTVYKGLDTETTVEVAWCELQTRKLTKAERQRFSEEVEMLKGLQHPNIVRFHDSWKSTMKGHKCIILVTELMTSGTLKTYLKRFKEMKLKLLQRWSRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKSASFAKSVIGTPEFMAPEMYEEKYDEAVDVYALGMCILEMATSEYPYSECQNAAQIYRKVTSGIKPDSFYKVKVPELKEIIEGCIRMNKDERYTIQDLLDHPFFQENNGVHVELAEEDDMVKSGLKLWLRMDDTKKLHGKYKDNNAIEFLFELYKDVPEEVAQEMVVLGFVCEADFKLVAKAIRDRVTIIKRQREKLRRQAEERKKKQAQEAIEEEPEHQAASPKVNDVVAADSPIPPLTPLTPLTPLTPLAPVLSPVTSSVDSGVSTHYPAEPEEPEVDQHFQVRHNSLSSANSDCETDGYLSPSGLQDPLEAGNVGMPANSPTTHPNPPAVNGISLRFPSSIAVSNNIEHGSSRPTSGFNSPVESYASDVTSSLSDGYEGLSEKNEKSVARRTAAKLFRRRARSRLRIIGLSDKVDRVVECQLQTHNDKMVTFKFDLDGDNPEDIAAVMVHNEFILPSEKEGFIYRLGDIIKRAEALFAKQQPVHSTGHRLAQPALHGGISSLSSSQPNLHNHALPRTHSSSSLPDCSVAKPSAGVRGLPPCPNGEVSSADITSPVRPLIRSQSFHNAPGSPLHNQHHHRPTSLLPNHQQYHLPFMNHTHFPFPYPGSPGSPKPAGIHPPLTRVASNPTFPSVPSPTPNSPSPVSETPSPVNTEFVNISPPALQTPTMWPPHSQPLFSLANVISMAMSMAQSFIPPAGLPTQGLPSYPSFLPHLHSHMAPQPGYPSVYPQPYQTSPVEASYPAGGIPVQNNVYYSPEHMPQMDGANLTHSNPPQWPHPAPPPSMAFTPPPPPPAQQVGLTGSPSRTQERSHMPPTSAQAPAPAKPRPEVSSSNSSSSSSDLSPSPPESPENTLSSFNSRLHPAVPAKNTSTPKPKASSPKSEVQSASVTVGRFQVTPSMDIPLPDAPAPAAPKPAENGSSQSESSTEEQGESEASLATSFTMTPPHPQPRKGSSGVQQEADGQAGWAVSQEQQQQEEEDEDEEEVIGEQPRGRRRSRRRAYSLSLLGTSADSGLSVTAADTEGRLWDGAADSPQYTNALHNLWMMTYNRSAPYLSSDDSDSEDEDMLEELQELREKHLAEVLALQAAQKREIEELYKKMGKVPPPGIVSPAAMLSSRQRRLSKGNGFPSSRRNSLQRLEMLPLQGIIRRNSLGGSSSGSQDKPFKGVTFATDLSRM

Target ORF information:

RefSeq Version	XM_014408459.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra serine/threonine-protein kinase WNK4 (LOC101477194), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408459.2

ORF Insert Sequence:

ATGTCTCATG CACAAGTAGA GATACCAGGA CGCGGGTTCC CGGGGCTCTC CGTGGATATG 
ACCGACGACT GTTTGTCCCG AGTCCCATCC GTGCTGAGGA CGCACGGGCT GGGCGCACGG 
AGGAACTCCT ACGGCCTGCA GAAAATTAGT ATGGACATTG ATTCTCCCCT ATACAGCGGT 
GTACTTTCCA AAGCCTTATC GTGGTCGGCA GAAAATATTC TCTCTCTGCC CGAGTCCAGA 
GCAGAGGAAG AAAAAACAGA CGACACCCCG CCGTCCGCGT CTCCGGTTTC CAGCTCCAGC 
ACGTGCCGCC AGGACCCCGA CGCCGGGTCC GTCGGTGGCA ACTGGAAACC TGTTGCCAGA 
TCCACTCCTC AAGATCCCAG CTCGGACTCT GAAAACGAAC TTCATAATAA ACAGCAAAAG 
ATTCTGTCTC GCATCACGTT TGACACTCAG TGGGAGCAGG AAGAAAAGGA GGACGTGGAG 
ACTAAAGCGG TGGCAACATC TCCTGATGGA AGATATCTCA AATTCAACAT AGAGATTGGA 
AGGGGGTCTT TCAAGACGGT CTATAAAGGG CTGGACACAG AGACTACAGT GGAGGTGGCA 
TGGTGTGAGC TACAGACTCG CAAGTTAACC AAGGCAGAGC GTCAGCGTTT CAGCGAGGAG 
GTGGAGATGC TAAAAGGTCT GCAGCATCCG AACATTGTGC GCTTCCACGA CTCCTGGAAG 
TCGACAATGA AGGGCCATAA GTGCATCATC CTGGTGACTG AACTCATGAC CTCAGGCACG 
CTCAAGACGT ACTTGAAGAG GTTCAAAGAG ATGAAGCTGA AGCTGCTCCA GCGCTGGAGT 
CGTCAGATCC TGAAAGGGCT GCACTTCCTG CACACACGCA CTCCTCCCAT CATCCACCGG 
GATCTCAAAT GTGATAATAT CTTCATCACT GGCCCCACAG GCTCTGTCAA GATTGGAGAT 
CTGGGGCTGG CCACTCTCAA AAGTGCCTCT TTTGCTAAAA GTGTCATTGG AACACCTGAG 
TTTATGGCCC CAGAGATGTA CGAGGAGAAA TATGATGAAG CGGTGGACGT CTACGCCTTA 
GGAATGTGCA TCCTGGAGAT GGCCACATCT GAGTACCCAT ATTCTGAGTG CCAAAATGCC 
GCCCAGATCT ACCGCAAAGT CACCAGTGGC ATTAAACCTG ACAGCTTCTA TAAAGTCAAA 
GTCCCAGAAC TTAAGGAGAT CATTGAGGGC TGCATCCGTA TGAACAAAGA TGAAAGGTAC 
ACCATTCAGG ACCTTCTGGA TCACCCCTTC TTCCAGGAAA ACAATGGCGT ACATGTGGAG 
TTGGCAGAGG AGGATGACAT GGTGAAGTCA GGCCTGAAGC TGTGGCTGCG CATGGACGAC 
ACCAAGAAGC TCCACGGGAA GTACAAGGAC AACAACGCCA TCGAGTTCCT CTTCGAGCTC 
TACAAAGATG TGCCTGAGGA GGTGGCACAG GAAATGGTTG TGCTTGGCTT TGTGTGTGAA 
GCAGACTTCA AACTAGTAGC GAAGGCTATA CGCGACCGAG TGACGATCAT CAAGCGTCAG 
AGAGAGAAAC TGAGGCGGCA GGCCGAGGAG AGGAAGAAGA AGCAGGCGCA AGAAGCCATA 
GAAGAAGAAC CAGAGCACCA GGCCGCTTCG CCCAAGGTCA ACGATGTTGT TGCAGCAGAT 
TCCCCCATCC CACCCCTGAC ACCTCTAACA CCTTTGACGC CATTGACACC ATTGGCCCCA 
GTTTTGTCCC CCGTCACCAG CTCTGTGGAC TCTGGCGTCA GCACCCACTA CCCTGCAGAG 
CCAGAGGAGC CCGAAGTTGA CCAGCACTTT CAAGTTCGCC ACAACAGCCT GTCTTCTGCT 
AACTCTGACT GCGAGACGGA CGGCTATCTG AGCCCCTCTG GTCTTCAGGA TCCGCTGGAG 
GCCGGCAACG TCGGCATGCC TGCGAACTCT CCCACCACGC ATCCCAACCC CCCCGCCGTA 
AACGGTATCT CCCTCCGCTT CCCCTCGAGT ATTGCTGTCT CCAACAACAT AGAACACGGC 
AGTTCAAGGC CTACGAGTGG CTTCAACTCC CCCGTGGAGA GCTACGCGTC TGATGTGACT 
TCAAGCCTGA GCGACGGCTA CGAGGGCCTA TCGGAGAAGA ACGAGAAATC AGTGGCGAGA 
CGAACGGCTG CGAAGCTGTT CAGGAGGAGG GCGCGCTCGA GGCTTCGCAT CATCGGGCTC 
TCTGATAAAG TGGACCGGGT GGTGGAGTGC CAGCTGCAGA CGCACAATGA CAAGATGGTG 
ACCTTCAAGT TCGACCTGGA TGGAGATAAC CCAGAAGACA TTGCTGCTGT CATGGTGCAC 
AACGAGTTCA TCTTGCCATC AGAGAAGGAG GGCTTTATCT ACCGCTTGGG TGACATCATC 
AAACGAGCCG AGGCACTGTT TGCCAAACAA CAGCCGGTCC ACTCCACTGG GCACCGACTT 
GCCCAGCCTG CATTGCACGG AGGGATTAGC TCACTGTCGT CCTCACAGCC TAATCTGCAC 
AATCACGCCC TGCCTCGAAC ACACTCGTCC TCTTCTCTGC CTGACTGCAG TGTGGCTAAA 
CCGAGTGCAG GGGTACGTGG CTTGCCCCCA TGTCCCAATG GAGAGGTCTC CAGTGCAGAC 
ATTACTTCAC CTGTGCGACC TCTAATACGC TCACAGTCTT TCCACAACGC CCCAGGTTCT 
CCTCTTCATA ATCAGCACCA TCACCGCCCT ACATCTCTCC TGCCTAATCA CCAGCAATAC 
CACTTGCCCT TTATGAACCA CACCCACTTT CCATTCCCAT ATCCAGGGTC TCCTGGTTCA 
CCCAAGCCAG CCGGGATCCA TCCTCCCCTC ACACGTGTAG CCAGTAACCC CACCTTCCCC 
TCTGTTCCCT CCCCAACCCC AAACTCGCCG AGTCCTGTGA GTGAGACCCC AAGTCCTGTC 
AACACGGAAT TTGTTAACAT ATCCCCTCCT GCCCTTCAAA CTCCCACCAT GTGGCCCCCC 
CACTCGCAGC CCCTTTTTTC CTTAGCCAAT GTAATTTCCA TGGCAATGAG CATGGCTCAG 
TCTTTTATCC CTCCTGCAGG CCTTCCCACC CAAGGGCTGC CCTCATATCC AAGCTTTCTC 
CCCCACCTAC ACAGTCATAT GGCTCCACAG CCAGGTTACC CCTCTGTCTA CCCTCAACCT 
TACCAGACTT CACCAGTAGA GGCCTCTTAC CCTGCAGGGG GGATCCCAGT GCAGAACAAT 
GTCTACTACA GCCCCGAGCA TATGCCTCAA ATGGATGGAG CTAATTTAAC TCACAGCAAT 
CCTCCACAGT GGCCCCATCC TGCCCCCCCA CCATCCATGG CTTTCACTCC TCCTCCACCT 
CCTCCTGCAC AGCAGGTTGG ACTCACCGGT TCCCCCAGCC GGACCCAGGA AAGGAGTCAC 
ATGCCCCCAA CCTCAGCCCA AGCTCCAGCT CCAGCTAAAC CCAGACCAGA GGTCTCCAGT 
TCGAATTCAA GTTCAAGCTC ATCAGATCTT TCACCATCAC CCCCAGAGAG CCCAGAAAAC 
ACTTTATCAT CTTTCAACAG CCGGTTGCAC CCTGCTGTGC CAGCGAAGAA TACAAGTACC 
CCCAAACCAA AAGCTTCTTC CCCCAAATCT GAAGTTCAGT CTGCATCAGT GACTGTTGGA 
CGATTCCAGG TGACGCCCAG CATGGACATC CCTTTGCCTG ATGCGCCTGC ACCTGCAGCC 
CCCAAGCCCG CAGAGAACGG CAGCAGCCAG TCGGAGAGCA GCACAGAGGA GCAGGGTGAG 
TCTGAGGCGA GCCTGGCCAC CTCCTTCACA ATGACCCCTC CTCACCCACA ACCCCGCAAA 
GGTTCTTCGG GAGTCCAACA GGAGGCTGAC GGGCAGGCTG GATGGGCAGT GAGCCAGGAG 
CAGCAGCAGC AGGAAGAGGA AGATGAAGAT GAGGAAGAGG TTATCGGGGA ACAGCCAAGG 
GGGAGGAGGA GAAGCCGTCG AAGGGCATAC AGCCTGAGCC TGTTGGGAAC GTCTGCAGAC 
AGTGGGCTCT CTGTGACGGC TGCTGATACA GAGGGGAGGC TGTGGGACGG AGCAGCGGAC 
AGTCCACAGT ACACCAATGC CCTTCACAAT TTATGGATGA TGACTTATAA CCGCAGTGCT 
CCCTACCTAA GCAGTGATGA CTCAGACAGT GAAGATGAGG ACATGCTGGA AGAGCTTCAG 
GAGCTCAGAG AGAAACATCT GGCAGAAGTT CTGGCCCTGC AGGCTGCCCA GAAAAGAGAA 
ATTGAAGAAC TATATAAGAA GATGGGAAAG GTCCCCCCTC CGGGCATCGT CTCTCCTGCT 
GCTATGTTGT CCAGCCGACA GCGCCGCCTC TCCAAAGGAA ACGGTTTTCC TTCGTCTCGC 
AGGAACAGCC TTCAGCGACT GGAGATGTTA CCGCTTCAAG GTATCATTAG GAGAAACTCT 
CTTGGAGGCA GCAGCAGTGG CTCGCAGGAC AAACCTTTTA AAGGCGTCAC TTTTGCCACA 
GACCTAAGTA GAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101477194 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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