ORF » Species Summary » Maylandia zebra » LOC101473709 cDNA ORF clone

LOC101473709 cDNA ORF clone, Maylandia zebra(zebra mbuna)

The following LOC101473709 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101473709 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMa23364 XM_004552536.1
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMa23365 XM_004552537.3
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMa23365 XM_004552538.2
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMa23365 XM_004552539.2
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMa357040 XM_004552536.2
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMa357041 XM_004552538.3
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMa357041 XM_004552537.4
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMa357041 XM_004552539.3
Latest version!		 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC101473709 ( XM_004552536.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552593.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMa23364
    Clone ID Related Accession (Same CDS sequence) XM_004552536.1
    Accession Version XM_004552536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1511884800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTTGGA TCAAGGACAC ACCTAAAACC TGCAGGACAC TGGCCCTTGA GACCTGGAGT 
    TCCCCAACAC TGATCATTAT GAGTGCAAAG CTGTGGACAG AGGAGCAGTT TAACTGCCCC 
    GTGTGTCTGG ATCTGCCAAA TGATCCTGTC ACCATCCCTT GCGGTCACAG CTACTGCATG 
    GCCTGCATTA AGGACTACTG GACTAAAGAT GACCCCAAAG GGATCTACAG CTGCCCTCAG 
    TGCCGTCAGA CCTTCTGCCC CAAGCCCTCC CTGTCCAGGA ACACCATGCT GGCAGAGGCT 
    GTGGAGCAGC TCCGCAAAGG AGCCCTCAAA TCTGAAGTGC GTCAATCCAT GCGACTCGCC 
    AAAGAGAAGC TTTCCTCCTC GGTCGTACCA TGTGACATGT GCAAAAATGA AAAACGAGCT 
    GCAGTCAAGA GCTGCCTGGT GTGTATGAGC TCCTACTGCG AGGCCCATCT GAAGCCTCAC 
    CAGACAAAGA AATCCCTAAA GCAGCATGAG CTCATCCCAC CGACAGGCAA CCTGGCCGAA 
    AAGATCTGCA CCCAGCACAA ATACCTGCAG GAGTTCGTCT GCCGACAGTG CAAGATGTTC 
    ATCTGCTGGC TGTGCACCAG CAACCAGCAC AAAGGCCACG AGAGCGTGTC CACCAAGGTC 
    GAAAGGCTGG AGAAGCAGAA ATTGCTGTCC GACCTGCAGT CAGCGAACCA ACAGAAGCTG 
    AAAGAGAGAG AGCAGGAGCT GAAAGACATG AAGAAGATGA TGGAGCAGAT GAAGCGTTCT 
    GCTGATGTGG TGCACAATGA AGCGGAGCAG GTGCTGTCGG AGCTGCTGCG CTCCATGGAA 
    CGTCTTCAGG AGCTGCTGGA GGAGGTGATG GATCAGGCCA ACCAGGAGAA GATGGGCCAT 
    GCACAAGAAG TTACAGATAG TCTGGAGGCT GAGATCAAGG AGCTGAAGAA AAGGGACACT 
    GAGATGAAGG ACCTGGCAAG CTGCGAAGAC AACATCTACT ATCTGCAGAC GTACGAGTCT 
    ATGTGCAGTC CTCTTGAGTC TGGGGATCTG CCAGCTGTGA AAGTCAATCA CGAGGCTTCC 
    TTTGAGCCTG TACGAGATGC CATCCTGGGT CTCAGGGAGC GAGTGGAAGA TCTATGCAAC 
    CAGGAGCTGG GCAAGATCAC TAAACAAGTG AATGACACAA CACTGTTCGC TCTGGGAGAC 
    TCCAACCGAA GCGGACAGAA AGGAGGGATT TTAAAATTGT TTTCTGGTCT CAGTGCCCGC 
    AACACAAACG CCCGTCCACA GGCTTCTACA CCCACTATCT CAATTGGAGG GCGAAGTACA 
    GACAAACGAG GACTTGGTAT AGGACTCAGG ACTCAAGATG TGAGGAGCAG AGACACAGCA 
    CGGGACAGAG CTAACACAAG CTCACCCAGA CTGCAGGACA AACAGAGGAG AGAAGACAGA 
    GATGCATTGA GGGAGCCTAA CCCCAGAGCC AGCACCAGAT CCAACATCAG CATCGGGTCC 
    AGCATCAGCC ACAGACTGAG CCCCAGAGCT AGCCCCACCC CCAGCCGCAG AGAATCTCAG 
    TCACTCTGGA GTAGGTCTAG CCAGAGCCAG GCCACAACTC CTACTCCAGT CCCAGCCAGC 
    CTCACTCCTG CTCCTACGCC TGCTCCTGCT CCAGCACGGG CATCTACTGG ACATTTTAGT 
    CGAATGGCGT CGATCAGCAG CATCTTCCGC TCCAATCGCC GTGGCACCAG CCAGGCTACT 
    CCAGCCGCAC AGGCCAGCAA CACACCGTCC GCAGGAGGAA ATCCATGGGG CATGGCTGAG 
    CTGTCTGAAA CTCCCACAGA AATTAACCCC GGTCTCTTTT TGGATACGCC CACCCCGGAC 
    TCCATGAATC ACGCTCCGGC TTTCCCCGCC TTGAGAGAAA TCAGCCTTGA CAGTATTCAA 
    GCCCCGGAGC CGAGGACTAG AGAGGAGTTC CTGCAGTACT CGGTGAGCTT GACCCTCGAC 
    TCCAACACGG CCCACAGGCA GCTGACGCTC TCTGAGAACA ACACTAAAGC CGCCCTGCAG 
    GGGACAGCCC AGCTTTACCC CGACAGCCCC CAGCGTTTTG ATGGCTGGAC CCAGGTGATG 
    TGTGACAGTC CGCTGTATGC CCAACGCTGC TACTGGGAGG TGGAGTGGAG AGGCCGGGGC 
    TCCTCAACCG GCATAGCTTA CGGGGCGATG GCCAGGAAGG GCTCGGACGC CAGGTCAGGC 
    CTCGGATACA ATGCCCAGTC CTGGACCCTG GAGCTGTCGG ACACCTGCTG CACAGCCATG 
    CATGACAATC AGAAAACGGA CATTCCGGTC GTGTACTGCC CCCGTGTGGG CATTTACCTG 
    GACCTGTCCG CGGGAACGCT GTCGTTCTAC AGCGTCGCAG AGAGCATGAC CCACCTCCAC 
    ACCTTCCGAG CCAACTTTAC GCAGCCGCTT TATGCCACCT TCTGGGTGGG GAGCGGAATC 
    GGGGTTGGGC TAGACTTCAG TTCAAGTTCT GAGAGCATCA AGATCTGCCC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552593.1
    CDS10..2526
    Translation

    Target ORF information:

    RefSeq Version XM_004552536.1
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTTGGA TCAAGGACAC ACCTAAAACC TGCAGGACAC TGGCCCTTGA GACCTGGAGT 
    TCCCCAACAC TGATCATTAT GAGTGCAAAG CTGTGGACAG AGGAGCAGTT TAACTGCCCC 
    GTGTGTCTGG ATCTGCCAAA TGATCCTGTC ACCATCCCTT GCGGTCACAG CTACTGCATG 
    GCCTGCATTA AGGACTACTG GACTAAAGAT GACCCCAAAG GGATCTACAG CTGCCCTCAG 
    TGCCGTCAGA CCTTCTGCCC CAAGCCCTCC CTGTCCAGGA ACACCATGCT GGCAGAGGCT 
    GTGGAGCAGC TCCGCAAAGG AGCCCTCAAA TCTGAAGTGC GTCAATCCAT GCGACTCGCC 
    AAAGAGAAGC TTTCCTCCTC GGTCGTACCA TGTGACATGT GCAAAAATGA AAAACGAGCT 
    GCAGTCAAGA GCTGCCTGGT GTGTATGAGC TCCTACTGCG AGGCCCATCT GAAGCCTCAC 
    CAGACAAAGA AATCCCTAAA GCAGCATGAG CTCATCCCAC CGACAGGCAA CCTGGCCGAA 
    AAGATCTGCA CCCAGCACAA ATACCTGCAG GAGTTCGTCT GCCGACAGTG CAAGATGTTC 
    ATCTGCTGGC TGTGCACCAG CAACCAGCAC AAAGGCCACG AGAGCGTGTC CACCAAGGTC 
    GAAAGGCTGG AGAAGCAGAA ATTGCTGTCC GACCTGCAGT CAGCGAACCA ACAGAAGCTG 
    AAAGAGAGAG AGCAGGAGCT GAAAGACATG AAGAAGATGA TGGAGCAGAT GAAGCGTTCT 
    GCTGATGTGG TGCACAATGA AGCGGAGCAG GTGCTGTCGG AGCTGCTGCG CTCCATGGAA 
    CGTCTTCAGG AGCTGCTGGA GGAGGTGATG GATCAGGCCA ACCAGGAGAA GATGGGCCAT 
    GCACAAGAAG TTACAGATAG TCTGGAGGCT GAGATCAAGG AGCTGAAGAA AAGGGACACT 
    GAGATGAAGG ACCTGGCAAG CTGCGAAGAC AACATCTACT ATCTGCAGAC GTACGAGTCT 
    ATGTGCAGTC CTCTTGAGTC TGGGGATCTG CCAGCTGTGA AAGTCAATCA CGAGGCTTCC 
    TTTGAGCCTG TACGAGATGC CATCCTGGGT CTCAGGGAGC GAGTGGAAGA TCTATGCAAC 
    CAGGAGCTGG GCAAGATCAC TAAACAAGTG AATGACACAA CACTGTTCGC TCTGGGAGAC 
    TCCAACCGAA GCGGACAGAA AGGAGGGATT TTAAAATTGT TTTCTGGTCT CAGTGCCCGC 
    AACACAAACG CCCGTCCACA GGCTTCTACA CCCACTATCT CAATTGGAGG GCGAAGTACA 
    GACAAACGAG GACTTGGTAT AGGACTCAGG ACTCAAGATG TGAGGAGCAG AGACACAGCA 
    CGGGACAGAG CTAACACAAG CTCACCCAGA CTGCAGGACA AACAGAGGAG AGAAGACAGA 
    GATGCATTGA GGGAGCCTAA CCCCAGAGCC AGCACCAGAT CCAACATCAG CATCGGGTCC 
    AGCATCAGCC ACAGACTGAG CCCCAGAGCT AGCCCCACCC CCAGCCGCAG AGAATCTCAG 
    TCACTCTGGA GTAGGTCTAG CCAGAGCCAG GCCACAACTC CTACTCCAGT CCCAGCCAGC 
    CTCACTCCTG CTCCTACGCC TGCTCCTGCT CCAGCACGGG CATCTACTGG ACATTTTAGT 
    CGAATGGCGT CGATCAGCAG CATCTTCCGC TCCAATCGCC GTGGCACCAG CCAGGCTACT 
    CCAGCCGCAC AGGCCAGCAA CACACCGTCC GCAGGAGGAA ATCCATGGGG CATGGCTGAG 
    CTGTCTGAAA CTCCCACAGA AATTAACCCC GGTCTCTTTT TGGATACGCC CACCCCGGAC 
    TCCATGAATC ACGCTCCGGC TTTCCCCGCC TTGAGAGAAA TCAGCCTTGA CAGTATTCAA 
    GCCCCGGAGC CGAGGACTAG AGAGGAGTTC CTGCAGTACT CGGTGAGCTT GACCCTCGAC 
    TCCAACACGG CCCACAGGCA GCTGACGCTC TCTGAGAACA ACACTAAAGC CGCCCTGCAG 
    GGGACAGCCC AGCTTTACCC CGACAGCCCC CAGCGTTTTG ATGGCTGGAC CCAGGTGATG 
    TGTGACAGTC CGCTGTATGC CCAACGCTGC TACTGGGAGG TGGAGTGGAG AGGCCGGGGC 
    TCCTCAACCG GCATAGCTTA CGGGGCGATG GCCAGGAAGG GCTCGGACGC CAGGTCAGGC 
    CTCGGATACA ATGCCCAGTC CTGGACCCTG GAGCTGTCGG ACACCTGCTG CACAGCCATG 
    CATGACAATC AGAAAACGGA CATTCCGGTC GTGTACTGCC CCCGTGTGGG CATTTACCTG 
    GACCTGTCCG CGGGAACGCT GTCGTTCTAC AGCGTCGCAG AGAGCATGAC CCACCTCCAC 
    ACCTTCCGAG CCAACTTTAC GCAGCCGCTT TATGCCACCT TCTGGGTGGG GAGCGGAATC 
    GGGGTTGGGC TAGACTTCAG TTCAAGTTCT GAGAGCATCA AGATCTGCCC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552538.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552595.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMa23365
    Clone ID Related Accession (Same CDS sequence) XM_004552538.2 , XM_004552539.2 , XM_004552537.3
    Accession Version XM_004552538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1511884800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004552538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552595.1
    CDS105..2543
    Translation

    Target ORF information:

    RefSeq Version XM_004552538.2
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552539.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552596.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMa23365
    Clone ID Related Accession (Same CDS sequence) XM_004552538.2 , XM_004552539.2 , XM_004552537.3
    Accession Version XM_004552539.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1511884800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004552539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552596.1
    CDS88..2526
    Translation

    Target ORF information:

    RefSeq Version XM_004552539.2
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552539.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552537.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552594.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMa23365
    Clone ID Related Accession (Same CDS sequence) XM_004552538.2 , XM_004552539.2 , XM_004552537.3
    Accession Version XM_004552537.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1511884800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004552537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552594.1
    CDS174..2612
    Translation

    Target ORF information:

    RefSeq Version XM_004552537.3
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552537.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACCCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552536.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552593.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMa357040
    Clone ID Related Accession (Same CDS sequence) XM_004552536.2
    Accession Version XM_004552536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1524412800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020192511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTTGGA TCAAGGACAC ACCTAAAACC TGCAGGACAC TGGCCCTTGA GACCTGGAGT 
    TCCCCAACAC TGATCATTAT GAGTGCAAAG CTGTGGACAG AGGAGCAGTT TAACTGCCCC 
    GTGTGTCTGG ATCTGCCAAA TGATCCTGTC ACCATCCCTT GCGGTCACAG CTACTGCATG 
    GCCTGCATTA AGGACTACTG GACTAAAGAT GACCCCAAAG GGATCTACAG CTGCCCTCAG 
    TGCCGTCAGA CCTTCTGCCC CAAGCCCTCC CTGTCCAGGA ACACCATGCT GGCAGAGGCT 
    GTGGAGCAGC TCCGCAAAGG AGCCCTCAAA TCTGAAGTGC GTCAATCCAT GCGACTCGCC 
    AAAGAGAAGC TTTCCTCCTC GGTCGTACCA TGTGACATGT GCAAAAATGA AAAACGAGCT 
    GCAGTCAAGA GCTGCCTGGT GTGTATGAGC TCCTACTGCG AGGCCCATCT GAAGCCTCAC 
    CAGACAAAGA AATCCCTAAA GCAGCATGAG CTCATCCCAC CGACAGGCAA CCTGGCCGAA 
    AAGATCTGCA CCCAGCACAA ATACCTGCAG GAGTTCGTCT GCCGACAGTG CAAGATGTTC 
    ATCTGCTGGC TGTGCACCAG CAACCAGCAC AAAGGCCACG AGAGCGTGTC CACCAAGGTC 
    GAAAGGCTGG AGAAGCAGAA ATTGCTGTCC GACCTGCAGT CAGCGAACCA ACAGAAGCTG 
    AAAGAGAGAG AGCAGGAGCT GAAAGACATG AAGAAGATGA TGGAGCAGAT GAAGCGTTCT 
    GCTGATGTGG TGCACAATGA AGCGGAGCAG GTGCTGTCGG AGCTGCTGCG CTCCATGGAA 
    CGTCTTCAGG AGCTGCTGGA GGAGGTGATG GATCAGGCCA ACCAGGAGAA GATGGGCCAT 
    GCACAAGAAG TTACAGATAG TCTGGAGGCT GAGATCAAGG AGCTGAAGAA AAGGGACACT 
    GAGATGAAGG ACCTGGCAAG CTGCGAAGAC AACATCTACT ATCTGCAGAC GTACGAGTCT 
    ATGTGCAGTC CTCTTGAGTC TGGGGATCTG CCAGCTGTGA AAGTCAATCA CGAGGCTTCC 
    TTTGAGCCTG TACGAGATGC CATCCTGGGT CTCAGGGAGC GAGTGGAAGA TCTATGCAAC 
    CAGGAGCTGG GCAAGATCAC TAAACAAGTG AATGACACAA CACTGTTCGC TCTGGGAGAC 
    TCCAACCGAA GCGGACAGAA AGGAGGGATT TTAAAATTGT TTTCTGGTCT CAGTGCCCGC 
    AACACAAACG CCCGTCCACA GGCTTCTACA CCCACTATCT CAATTGGAGG GCGAAGTACA 
    GACAAACGAG GACTTGGTAT AGGACTCAGG ACTCAAGATG TGAGGAGCAG AGACACAGCA 
    CGGGACAGAG CTAACACAAG CTCACCCAGA CTGCAGGACA AACAGAGGAG AGAAGACAGA 
    GATGCATTGA GGGAGCCTAA CCCCAGAGCC AGCACCAGAT CCAACATCAG CATCGGGTCC 
    AGCATCAGCC ACAGACTGAG CCCCAGAGCT AGCCCCACCC CCAGCCGCAG AGAATCTCAG 
    TCACTCTGGA GTAGGTCTAG CCAGAGCCAG GCCACAACTC CTACTCCAGT CCCAGCCAGC 
    CTCACTCCTG CTCCTACGCC TGCTCCTGCT CCAGCACGGG CATCTACTGG ACATTTTAGT 
    CGAATGGCGT CGATCAGCAG CATCTTCCGC TCCAATCGCC GTGGCACCAG CCAGGCTACT 
    CCAGCCGCAC AGGCCAGCAA CACACCGTCC GCAGGAGGAA ATCCATGGGG CATGGCTGAG 
    CTGTCTGAAA CTCCCACAGA AATTAACCCC GGTCTCTTTT TGGATACGCC CACTCCGGAC 
    TCCATGAATC ACGCTCCGGC TTTCCCCGCC TTGAGAGAAA TCAGCCTTGA CAGTATTCAA 
    GCCCCGGAGC CGAGGACTAG AGAGGAGTTC CTGCAGTACT CGGTGAGCTT GACCCTCGAC 
    TCCAACACGG CCCACAGGCA GCTGACGCTC TCTGAGAACA ACACTAAAGC CGCCCTGCAG 
    GGGACAGCCC AGCTTTACCC CGACAGCCCC CAGCGTTTTG ATGGCTGGAC CCAGGTGATG 
    TGTGACAGTC CGCTGTATGC CCAACGCTGC TACTGGGAGG TGGAGTGGAG AGGCCGGGGC 
    TCCTCAACCG GCATAGCTTA CGGGGCGATG GCCAGGAAGG GCTCGGACGC CAGGTCAGGC 
    CTCGGATACA ATGCCCAGTC CTGGACCCTG GAGCTGTCGG ACACCTGCTG CACAGCCATG 
    CATGACAATC AGAAAACGGA CATTCCGGTC GTGTACTGCC CCCGTGTGGG CATTTACCTG 
    GACCTGTCCG CGGGAACGCT GTCGTTCTAC AGCGTCGCAG AGAGCATGAC CCACCTCCAC 
    ACCTTCCGAG CCAACTTTAC GCAGCCGCTT TATGCCACCT TCTGGGTGGG GAGCGGAATC 
    GGGGTTGGGC TAGACTTCAG TTCAAGTTCT GAGAGCATCA AGATCTGCCC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552593.1
    CDS10..2526
    Translation

    Target ORF information:

    RefSeq Version XM_004552536.2
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTGTTGGA TCAAGGACAC ACCTAAAACC TGCAGGACAC TGGCCCTTGA GACCTGGAGT 
    TCCCCAACAC TGATCATTAT GAGTGCAAAG CTGTGGACAG AGGAGCAGTT TAACTGCCCC 
    GTGTGTCTGG ATCTGCCAAA TGATCCTGTC ACCATCCCTT GCGGTCACAG CTACTGCATG 
    GCCTGCATTA AGGACTACTG GACTAAAGAT GACCCCAAAG GGATCTACAG CTGCCCTCAG 
    TGCCGTCAGA CCTTCTGCCC CAAGCCCTCC CTGTCCAGGA ACACCATGCT GGCAGAGGCT 
    GTGGAGCAGC TCCGCAAAGG AGCCCTCAAA TCTGAAGTGC GTCAATCCAT GCGACTCGCC 
    AAAGAGAAGC TTTCCTCCTC GGTCGTACCA TGTGACATGT GCAAAAATGA AAAACGAGCT 
    GCAGTCAAGA GCTGCCTGGT GTGTATGAGC TCCTACTGCG AGGCCCATCT GAAGCCTCAC 
    CAGACAAAGA AATCCCTAAA GCAGCATGAG CTCATCCCAC CGACAGGCAA CCTGGCCGAA 
    AAGATCTGCA CCCAGCACAA ATACCTGCAG GAGTTCGTCT GCCGACAGTG CAAGATGTTC 
    ATCTGCTGGC TGTGCACCAG CAACCAGCAC AAAGGCCACG AGAGCGTGTC CACCAAGGTC 
    GAAAGGCTGG AGAAGCAGAA ATTGCTGTCC GACCTGCAGT CAGCGAACCA ACAGAAGCTG 
    AAAGAGAGAG AGCAGGAGCT GAAAGACATG AAGAAGATGA TGGAGCAGAT GAAGCGTTCT 
    GCTGATGTGG TGCACAATGA AGCGGAGCAG GTGCTGTCGG AGCTGCTGCG CTCCATGGAA 
    CGTCTTCAGG AGCTGCTGGA GGAGGTGATG GATCAGGCCA ACCAGGAGAA GATGGGCCAT 
    GCACAAGAAG TTACAGATAG TCTGGAGGCT GAGATCAAGG AGCTGAAGAA AAGGGACACT 
    GAGATGAAGG ACCTGGCAAG CTGCGAAGAC AACATCTACT ATCTGCAGAC GTACGAGTCT 
    ATGTGCAGTC CTCTTGAGTC TGGGGATCTG CCAGCTGTGA AAGTCAATCA CGAGGCTTCC 
    TTTGAGCCTG TACGAGATGC CATCCTGGGT CTCAGGGAGC GAGTGGAAGA TCTATGCAAC 
    CAGGAGCTGG GCAAGATCAC TAAACAAGTG AATGACACAA CACTGTTCGC TCTGGGAGAC 
    TCCAACCGAA GCGGACAGAA AGGAGGGATT TTAAAATTGT TTTCTGGTCT CAGTGCCCGC 
    AACACAAACG CCCGTCCACA GGCTTCTACA CCCACTATCT CAATTGGAGG GCGAAGTACA 
    GACAAACGAG GACTTGGTAT AGGACTCAGG ACTCAAGATG TGAGGAGCAG AGACACAGCA 
    CGGGACAGAG CTAACACAAG CTCACCCAGA CTGCAGGACA AACAGAGGAG AGAAGACAGA 
    GATGCATTGA GGGAGCCTAA CCCCAGAGCC AGCACCAGAT CCAACATCAG CATCGGGTCC 
    AGCATCAGCC ACAGACTGAG CCCCAGAGCT AGCCCCACCC CCAGCCGCAG AGAATCTCAG 
    TCACTCTGGA GTAGGTCTAG CCAGAGCCAG GCCACAACTC CTACTCCAGT CCCAGCCAGC 
    CTCACTCCTG CTCCTACGCC TGCTCCTGCT CCAGCACGGG CATCTACTGG ACATTTTAGT 
    CGAATGGCGT CGATCAGCAG CATCTTCCGC TCCAATCGCC GTGGCACCAG CCAGGCTACT 
    CCAGCCGCAC AGGCCAGCAA CACACCGTCC GCAGGAGGAA ATCCATGGGG CATGGCTGAG 
    CTGTCTGAAA CTCCCACAGA AATTAACCCC GGTCTCTTTT TGGATACGCC CACTCCGGAC 
    TCCATGAATC ACGCTCCGGC TTTCCCCGCC TTGAGAGAAA TCAGCCTTGA CAGTATTCAA 
    GCCCCGGAGC CGAGGACTAG AGAGGAGTTC CTGCAGTACT CGGTGAGCTT GACCCTCGAC 
    TCCAACACGG CCCACAGGCA GCTGACGCTC TCTGAGAACA ACACTAAAGC CGCCCTGCAG 
    GGGACAGCCC AGCTTTACCC CGACAGCCCC CAGCGTTTTG ATGGCTGGAC CCAGGTGATG 
    TGTGACAGTC CGCTGTATGC CCAACGCTGC TACTGGGAGG TGGAGTGGAG AGGCCGGGGC 
    TCCTCAACCG GCATAGCTTA CGGGGCGATG GCCAGGAAGG GCTCGGACGC CAGGTCAGGC 
    CTCGGATACA ATGCCCAGTC CTGGACCCTG GAGCTGTCGG ACACCTGCTG CACAGCCATG 
    CATGACAATC AGAAAACGGA CATTCCGGTC GTGTACTGCC CCCGTGTGGG CATTTACCTG 
    GACCTGTCCG CGGGAACGCT GTCGTTCTAC AGCGTCGCAG AGAGCATGAC CCACCTCCAC 
    ACCTTCCGAG CCAACTTTAC GCAGCCGCTT TATGCCACCT TCTGGGTGGG GAGCGGAATC 
    GGGGTTGGGC TAGACTTCAG TTCAAGTTCT GAGAGCATCA AGATCTGCCC CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552538.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552595.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMa357041
    Clone ID Related Accession (Same CDS sequence) XM_004552538.3 , XM_004552537.4 , XM_004552539.3
    Accession Version XM_004552538.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1524412800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020192511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552595.1
    CDS105..2543
    Translation

    Target ORF information:

    RefSeq Version XM_004552538.3
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552538.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552537.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552594.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMa357041
    Clone ID Related Accession (Same CDS sequence) XM_004552538.3 , XM_004552537.4 , XM_004552539.3
    Accession Version XM_004552537.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1524412800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020192511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552537.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552594.1
    CDS174..2612
    Translation

    Target ORF information:

    RefSeq Version XM_004552537.4
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552537.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101473709 ( XM_004552539.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004552596.1 Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMa357041
    Clone ID Related Accession (Same CDS sequence) XM_004552538.3 , XM_004552537.4 , XM_004552539.3
    Accession Version XM_004552539.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1524412800000
    Organism Maylandia zebra(zebra mbuna)
    Product E3 ubiquitin/ISG15 ligase TRIM25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020192511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004552539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004552596.1
    CDS88..2526
    Translation

    Target ORF information:

    RefSeq Version XM_004552539.3
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra E3 ubiquitin/ISG15 ligase TRIM25 (LOC101473709), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004552539.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAGTGCAA AGCTGTGGAC AGAGGAGCAG TTTAACTGCC CCGTGTGTCT GGATCTGCCA 
    AATGATCCTG TCACCATCCC TTGCGGTCAC AGCTACTGCA TGGCCTGCAT TAAGGACTAC 
    TGGACTAAAG ATGACCCCAA AGGGATCTAC AGCTGCCCTC AGTGCCGTCA GACCTTCTGC 
    CCCAAGCCCT CCCTGTCCAG GAACACCATG CTGGCAGAGG CTGTGGAGCA GCTCCGCAAA 
    GGAGCCCTCA AATCTGAAGT GCGTCAATCC ATGCGACTCG CCAAAGAGAA GCTTTCCTCC 
    TCGGTCGTAC CATGTGACAT GTGCAAAAAT GAAAAACGAG CTGCAGTCAA GAGCTGCCTG 
    GTGTGTATGA GCTCCTACTG CGAGGCCCAT CTGAAGCCTC ACCAGACAAA GAAATCCCTA 
    AAGCAGCATG AGCTCATCCC ACCGACAGGC AACCTGGCCG AAAAGATCTG CACCCAGCAC 
    AAATACCTGC AGGAGTTCGT CTGCCGACAG TGCAAGATGT TCATCTGCTG GCTGTGCACC 
    AGCAACCAGC ACAAAGGCCA CGAGAGCGTG TCCACCAAGG TCGAAAGGCT GGAGAAGCAG 
    AAATTGCTGT CCGACCTGCA GTCAGCGAAC CAACAGAAGC TGAAAGAGAG AGAGCAGGAG 
    CTGAAAGACA TGAAGAAGAT GATGGAGCAG ATGAAGCGTT CTGCTGATGT GGTGCACAAT 
    GAAGCGGAGC AGGTGCTGTC GGAGCTGCTG CGCTCCATGG AACGTCTTCA GGAGCTGCTG 
    GAGGAGGTGA TGGATCAGGC CAACCAGGAG AAGATGGGCC ATGCACAAGA AGTTACAGAT 
    AGTCTGGAGG CTGAGATCAA GGAGCTGAAG AAAAGGGACA CTGAGATGAA GGACCTGGCA 
    AGCTGCGAAG ACAACATCTA CTATCTGCAG ACGTACGAGT CTATGTGCAG TCCTCTTGAG 
    TCTGGGGATC TGCCAGCTGT GAAAGTCAAT CACGAGGCTT CCTTTGAGCC TGTACGAGAT 
    GCCATCCTGG GTCTCAGGGA GCGAGTGGAA GATCTATGCA ACCAGGAGCT GGGCAAGATC 
    ACTAAACAAG TGAATGACAC AACACTGTTC GCTCTGGGAG ACTCCAACCG AAGCGGACAG 
    AAAGGAGGGA TTTTAAAATT GTTTTCTGGT CTCAGTGCCC GCAACACAAA CGCCCGTCCA 
    CAGGCTTCTA CACCCACTAT CTCAATTGGA GGGCGAAGTA CAGACAAACG AGGACTTGGT 
    ATAGGACTCA GGACTCAAGA TGTGAGGAGC AGAGACACAG CACGGGACAG AGCTAACACA 
    AGCTCACCCA GACTGCAGGA CAAACAGAGG AGAGAAGACA GAGATGCATT GAGGGAGCCT 
    AACCCCAGAG CCAGCACCAG ATCCAACATC AGCATCGGGT CCAGCATCAG CCACAGACTG 
    AGCCCCAGAG CTAGCCCCAC CCCCAGCCGC AGAGAATCTC AGTCACTCTG GAGTAGGTCT 
    AGCCAGAGCC AGGCCACAAC TCCTACTCCA GTCCCAGCCA GCCTCACTCC TGCTCCTACG 
    CCTGCTCCTG CTCCAGCACG GGCATCTACT GGACATTTTA GTCGAATGGC GTCGATCAGC 
    AGCATCTTCC GCTCCAATCG CCGTGGCACC AGCCAGGCTA CTCCAGCCGC ACAGGCCAGC 
    AACACACCGT CCGCAGGAGG AAATCCATGG GGCATGGCTG AGCTGTCTGA AACTCCCACA 
    GAAATTAACC CCGGTCTCTT TTTGGATACG CCCACTCCGG ACTCCATGAA TCACGCTCCG 
    GCTTTCCCCG CCTTGAGAGA AATCAGCCTT GACAGTATTC AAGCCCCGGA GCCGAGGACT 
    AGAGAGGAGT TCCTGCAGTA CTCGGTGAGC TTGACCCTCG ACTCCAACAC GGCCCACAGG 
    CAGCTGACGC TCTCTGAGAA CAACACTAAA GCCGCCCTGC AGGGGACAGC CCAGCTTTAC 
    CCCGACAGCC CCCAGCGTTT TGATGGCTGG ACCCAGGTGA TGTGTGACAG TCCGCTGTAT 
    GCCCAACGCT GCTACTGGGA GGTGGAGTGG AGAGGCCGGG GCTCCTCAAC CGGCATAGCT 
    TACGGGGCGA TGGCCAGGAA GGGCTCGGAC GCCAGGTCAG GCCTCGGATA CAATGCCCAG 
    TCCTGGACCC TGGAGCTGTC GGACACCTGC TGCACAGCCA TGCATGACAA TCAGAAAACG 
    GACATTCCGG TCGTGTACTG CCCCCGTGTG GGCATTTACC TGGACCTGTC CGCGGGAACG 
    CTGTCGTTCT ACAGCGTCGC AGAGAGCATG ACCCACCTCC ACACCTTCCG AGCCAACTTT 
    ACGCAGCCGC TTTATGCCAC CTTCTGGGTG GGGAGCGGAA TCGGGGTTGG GCTAGACTTC 
    AGTTCAAGTT CTGAGAGCAT CAAGATCTGC CCCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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