ORF » Species Summary » Maylandia zebra » LOC101472221 cDNA ORF clone

LOC101472221 cDNA ORF clone, Maylandia zebra(zebra mbuna)

The following LOC101472221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101472221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMa22502 XM_004548515.4
Latest version!		 Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OMa22502 XM_004548515.3 Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC101472221 ( XM_004548515.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004548572.1 Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA.
    Price & Availability↑
    CloneID OMa22502
    Clone ID Related Accession (Same CDS sequence) XM_004548515.3 , XM_004548515.4
    Accession Version XM_004548515.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1511884800000
    Organism Maylandia zebra(zebra mbuna)
    Product voltage-dependent calcium channel subunit alpha-2/delta-2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447316.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004548515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGATAG GCGAAAGGTC TTGCAGCATC GTCTGCTTGC TATCTATCCA GATTATCTTC 
    ATTTTCAGCG CATCGTGGCC TGGAGCGCAG GGTCTCTTCC CCCAGCAATA CACGATGATG 
    CACTGGGCCA GGCGTATCGA GCAGGAGATT GACAGAGTCT TTCAGCACAT CACTGGAGCT 
    CAGCAGTTGA AAGGGATATA CAATGAAGAA AGGCGTCGGT TTAGTCTGGT GAAGAATCAG 
    CCTCGCAAGA TTGTTGAAAA AGTGGCCTCG GATATTGAGA AACTTCTGGC TAAAAAGCGC 
    AAAGCACTTG ATAGGTTAGC CAGTGAAGCA GAGCGGCTCC AGCGGGAGCA TCTGTGGCAG 
    GATGAAATCA AGGAGCTGGA CATGGCTTAT TATGATTCCA AGGCAGAGCT GGATTATTAT 
    TCCATGGATG GAGAAGGAGA GGTGGAAAAT CCCTCTCACA TCAAGCTGGA GTTTGTGTAT 
    GACCCAAACT TCAAGAATAA TGTCAACTAT TCCTACACAG CTGTTCAGAT TCCCACGGAT 
    ATTTATAAAG GAGCTCCCGT CATTCTAAAC GAACTGAACT GGACGCAGGC GTTGGAGAAA 
    GTCTTCATGG AGAATAGTCG AGAGGATCCG TCACTGCTGT GGCAAGCATT TGGGAGCGCG 
    ACAGGAGTCA CTCGCTATTA TCCAGCAACA CCCTGGAAAG CTCCTGATAA AATTGACCTG 
    TATGATGTCA GGAGGAGGCC CTGGTACATC CAGGGTGCCT CATCCCCCAA AGACATGGTC 
    ATCCTTGTTG ACGTGAGTGG CAGTGTCAGT GGACTCACCC TGAAACTGAT CAAAGCGTCA 
    GTGATGGAAA TGCTGGACAC TTTATCCGAC GATGACTACG TCAATGTGGC CAGGTTTAAT 
    GAGAAGGCTG TGGCTGTGGT CCCTTGCTTT AGCCATCTAG TCCAGGCTAA TGTGCGCAAC 
    AAAAAGATTT TCAAGGATGC AGTGCAGCAG ATGCAGGCTA AAGGCACCAC TGACTACAAG 
    TCTGGATTTC ACTTTGCCTT CAATCAGCTA TTAAATAAAA CAAATGTCCC CCGGGCTAAC 
    TGCAATAAAA TAATCATGCT GTTTACTGAC GGAGGAGAGG ACCGAGCTCA GGATGTTTTC 
    ATGCAATACA ACTGGCCCAA CAAAACGGTT CGAGTATTTA CCTTTTCTGT GGGCCAGCAT 
    AACTATGATG TCACACCCTT ACAGTGGATT GCGTGCACCA ATAAAGGTTA CTATTTCGAG 
    ATCCGTTCCA TCTGCGCTAT AAGGATTAAC ACCCAGGAGT ACCTTGACGT GCTGGGACGC 
    CCCATGGTTC TGGCAGGCAG CGAAGCCAAG CAGGTTCAGT GGACCAATGT GTATCAGGAT 
    GCTCTGGGTC TTGGCATGGT AGTAACTGGG ACTTTGCCTG TTTTTAATCT CACCATGGAC 
    GGAAACTCAC AGAATCAGCT GATTTTAGGT GTCATGGGAG TCGACGTGCA TCTTGATGAG 
    ATAAAGCGAC TCACACCGCG GTACAATCTC GGGGCCAACG GATACATATT TGCCATCGAT 
    CCAAACGGAT ACCTGCTCCT TCATCCTAAT CTCCAGCCAA AGCTTGTGAA TCTGCCAGAA 
    CCTGTGACGC TGGACTTCCT GGACGCAGAG TTGGAGGACA GCAATAAAGA AGAGATCCGC 
    CGACAGATGA TCGATGGAAG ACCAGGTGAA ATGCAGATCA AAACTCTGGT ACTGTCGATT 
    GACGAGCGCT ACATCGATGA GGTCTACAGG GGTTACACCT GGACTCCTAT CAATGGAACA 
    GACTACAGTC TCGGTCTAGT ATTACCATCC TACAATGAGT ACTACATCAA GGCAGATCTG 
    AGCGATGTGA TGCTGCAGCT CCAGTATATG CAGTCTTTGC TGCCCAGCTC CTTTGAATCA 
    TCAGGGCACG TGTTTCTAGC TCCAAGGGAA TACTGCAAGC GTCTGCAGCT TTCTGACAAC 
    AACACCCAGT TCTTGCAGAA CTTTCTGTCT CTCATGCTTG ACCTCTCCCC TGAATCTGAT 
    GAGTGTGACC ATGGCCTGAT CCACAACCTG ATTTTTGATT CTAGGATCAT CGGGCAGCTT 
    GCTTCTCGTG TATGGAAGAA CAAGGACCTC AATTCGTATG GCTTCCTAGC TGTATTTGCA 
    TCCACAGACG GAGGAATAAC ACGAGTTTTT CCCAACATAG CTGCCGAGTT GTGGGATGAG 
    GACCCTGAAC CCTTCAATTC AAATTTCTAC AGACGGAGCC TCGACAACAA AGGCTACATG 
    TTCAGATCTA CACTCAGATC CTCTTTGGAT GACCCCATTG CAGCAGAAAA TGGGACGAGT 
    GAAATTGTGG TTAGTTCAGC TGTGGAGGTT AATTTAGCAG GAAAACTGCT CAGACCTGCA 
    GTGGTTGGTG GGAAGCTGGA CTTGGAAGCT TGGGTGGATA AGTTCAAAAT CCTGGCCAGC 
    AACATGTCAG ACGGCCGGCA GGGGTCACAC AAGTGTGGAC CTCTGAGAAG TTGCGAGATG 
    GATTGCGAGA TGGACACAAA TGAACTCGGC TGCTATCTCA TTGATGATGG TGGGTTCCTG 
    GTTATGTCCA ATCAGGCAGA TCACTTCCAA AAGATTGGTC TTTTCTTTGG CGATGTGGAC 
    GCTCCCCTGA TGTATGCCCT CTACAACAAT TCGATCTTCA CTCGACGGCA GTCTTTCCAG 
    TACCAGTCTG CATGTGAGCC GGTCAGCAGC AGCCACACCG GCGCAGCACC GAGAGGTGTT 
    TTTGTGCCGT CCATTGCTGA TGTTTTGAGC TTGGCATGGT GGACATCAAC AATGGCATGG 
    TCTGTGTTTC AGCAGCTTCT TTATGGACTG GCCTATAACA GCTGGCTCTA TCAAGCTGAT 
    GTCCTCATCG AAGGCTTTGA GACTAAAGAA AGCAGCTGTG TGACCATCCA AAGCCAGTTC 
    TACTTCACAA ACACCACAAA CTCCTACAAT ATGCTGCAGG ACTGTGGAAA CTGCTCCAGA 
    CTGTTCCATG CAAAGAGGAT CGAAAACACA AACCTGCTTT TTGTGGTCGC CGAGAAACTG 
    CTCTGCCAGT CGTGTGAGAT TGAGACACTG ACCCAGGTCA GAACAGAGTT TCAGGAGGAA 
    AATCCGTGTG AAGTATTGAA CAATACACGA TATCGTAAAG GCCCGACTTC CTGCTTTGAC 
    TACAGTGCCT TAGAAAACAC GTCAGAGTGT GGACGAGGTC ATTCCCTGCA GTCCTCCATA 
    GGAGTCCTCC TCTTCATTCA GCTCACTCTG CCTCTCTTTC ATCTCTCACA CATGCAAACG 
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004548572.1
    CDS321..3626
    Translation

    Target ORF information:

    RefSeq Version XM_004548515.3
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004548515.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGATAG GCGAAAGGTC TTGCAGCATC GTCTGCTTGC TATCTATCCA GATTATCTTC 
    ATTTTCAGCG CATCGTGGCC TGGAGCGCAG GGTCTCTTCC CCCAGCAATA CACGATGATG 
    CACTGGGCCA GGCGTATCGA GCAGGAGATT GACAGAGTCT TTCAGCACAT CACTGGAGCT 
    CAGCAGTTGA AAGGGATATA CAATGAAGAA AGGCGTCGGT TTAGTCTGGT GAAGAATCAG 
    CCTCGCAAGA TTGTTGAAAA AGTGGCCTCG GATATTGAGA AACTTCTGGC TAAAAAGCGC 
    AAAGCACTTG ATAGGTTAGC CAGTGAAGCA GAGCGGCTCC AGCGGGAGCA TCTGTGGCAG 
    GATGAAATCA AGGAGCTGGA CATGGCTTAT TATGATTCCA AGGCAGAGCT GGATTATTAT 
    TCCATGGATG GAGAAGGAGA GGTGGAAAAT CCCTCTCACA TCAAGCTGGA GTTTGTGTAT 
    GACCCAAACT TCAAGAATAA TGTCAACTAT TCCTACACAG CTGTTCAGAT TCCCACGGAT 
    ATTTATAAAG GAGCTCCCGT CATTCTAAAC GAACTGAACT GGACGCAGGC GTTGGAGAAA 
    GTCTTCATGG AGAATAGTCG AGAGGATCCG TCACTGCTGT GGCAAGCATT TGGGAGCGCG 
    ACAGGAGTCA CTCGCTATTA TCCAGCAACA CCCTGGAAAG CTCCTGATAA AATTGACCTG 
    TATGATGTCA GGAGGAGGCC CTGGTACATC CAGGGTGCCT CATCCCCCAA AGACATGGTC 
    ATCCTTGTTG ACGTGAGTGG CAGTGTCAGT GGACTCACCC TGAAACTGAT CAAAGCGTCA 
    GTGATGGAAA TGCTGGACAC TTTATCCGAC GATGACTACG TCAATGTGGC CAGGTTTAAT 
    GAGAAGGCTG TGGCTGTGGT CCCTTGCTTT AGCCATCTAG TCCAGGCTAA TGTGCGCAAC 
    AAAAAGATTT TCAAGGATGC AGTGCAGCAG ATGCAGGCTA AAGGCACCAC TGACTACAAG 
    TCTGGATTTC ACTTTGCCTT CAATCAGCTA TTAAATAAAA CAAATGTCCC CCGGGCTAAC 
    TGCAATAAAA TAATCATGCT GTTTACTGAC GGAGGAGAGG ACCGAGCTCA GGATGTTTTC 
    ATGCAATACA ACTGGCCCAA CAAAACGGTT CGAGTATTTA CCTTTTCTGT GGGCCAGCAT 
    AACTATGATG TCACACCCTT ACAGTGGATT GCGTGCACCA ATAAAGGTTA CTATTTCGAG 
    ATCCGTTCCA TCTGCGCTAT AAGGATTAAC ACCCAGGAGT ACCTTGACGT GCTGGGACGC 
    CCCATGGTTC TGGCAGGCAG CGAAGCCAAG CAGGTTCAGT GGACCAATGT GTATCAGGAT 
    GCTCTGGGTC TTGGCATGGT AGTAACTGGG ACTTTGCCTG TTTTTAATCT CACCATGGAC 
    GGAAACTCAC AGAATCAGCT GATTTTAGGT GTCATGGGAG TCGACGTGCA TCTTGATGAG 
    ATAAAGCGAC TCACACCGCG GTACAATCTC GGGGCCAACG GATACATATT TGCCATCGAT 
    CCAAACGGAT ACCTGCTCCT TCATCCTAAT CTCCAGCCAA AGCTTGTGAA TCTGCCAGAA 
    CCTGTGACGC TGGACTTCCT GGACGCAGAG TTGGAGGACA GCAATAAAGA AGAGATCCGC 
    CGACAGATGA TCGATGGAAG ACCAGGTGAA ATGCAGATCA AAACTCTGGT ACTGTCGATT 
    GACGAGCGCT ACATCGATGA GGTCTACAGG GGTTACACCT GGACTCCTAT CAATGGAACA 
    GACTACAGTC TCGGTCTAGT ATTACCATCC TACAATGAGT ACTACATCAA GGCAGATCTG 
    AGCGATGTGA TGCTGCAGCT CCAGTATATG CAGTCTTTGC TGCCCAGCTC CTTTGAATCA 
    TCAGGGCACG TGTTTCTAGC TCCAAGGGAA TACTGCAAGC GTCTGCAGCT TTCTGACAAC 
    AACACCCAGT TCTTGCAGAA CTTTCTGTCT CTCATGCTTG ACCTCTCCCC TGAATCTGAT 
    GAGTGTGACC ATGGCCTGAT CCACAACCTG ATTTTTGATT CTAGGATCAT CGGGCAGCTT 
    GCTTCTCGTG TATGGAAGAA CAAGGACCTC AATTCGTATG GCTTCCTAGC TGTATTTGCA 
    TCCACAGACG GAGGAATAAC ACGAGTTTTT CCCAACATAG CTGCCGAGTT GTGGGATGAG 
    GACCCTGAAC CCTTCAATTC AAATTTCTAC AGACGGAGCC TCGACAACAA AGGCTACATG 
    TTCAGATCTA CACTCAGATC CTCTTTGGAT GACCCCATTG CAGCAGAAAA TGGGACGAGT 
    GAAATTGTGG TTAGTTCAGC TGTGGAGGTT AATTTAGCAG GAAAACTGCT CAGACCTGCA 
    GTGGTTGGTG GGAAGCTGGA CTTGGAAGCT TGGGTGGATA AGTTCAAAAT CCTGGCCAGC 
    AACATGTCAG ACGGCCGGCA GGGGTCACAC AAGTGTGGAC CTCTGAGAAG TTGCGAGATG 
    GATTGCGAGA TGGACACAAA TGAACTCGGC TGCTATCTCA TTGATGATGG TGGGTTCCTG 
    GTTATGTCCA ATCAGGCAGA TCACTTCCAA AAGATTGGTC TTTTCTTTGG CGATGTGGAC 
    GCTCCCCTGA TGTATGCCCT CTACAACAAT TCGATCTTCA CTCGACGGCA GTCTTTCCAG 
    TACCAGTCTG CATGTGAGCC GGTCAGCAGC AGCCACACCG GCGCAGCACC GAGAGGTGTT 
    TTTGTGCCGT CCATTGCTGA TGTTTTGAGC TTGGCATGGT GGACATCAAC AATGGCATGG 
    TCTGTGTTTC AGCAGCTTCT TTATGGACTG GCCTATAACA GCTGGCTCTA TCAAGCTGAT 
    GTCCTCATCG AAGGCTTTGA GACTAAAGAA AGCAGCTGTG TGACCATCCA AAGCCAGTTC 
    TACTTCACAA ACACCACAAA CTCCTACAAT ATGCTGCAGG ACTGTGGAAA CTGCTCCAGA 
    CTGTTCCATG CAAAGAGGAT CGAAAACACA AACCTGCTTT TTGTGGTCGC CGAGAAACTG 
    CTCTGCCAGT CGTGTGAGAT TGAGACACTG ACCCAGGTCA GAACAGAGTT TCAGGAGGAA 
    AATCCGTGTG AAGTATTGAA CAATACACGA TATCGTAAAG GCCCGACTTC CTGCTTTGAC 
    TACAGTGCCT TAGAAAACAC GTCAGAGTGT GGACGAGGTC ATTCCCTGCA GTCCTCCATA 
    GGAGTCCTCC TCTTCATTCA GCTCACTCTG CCTCTCTTTC ATCTCTCACA CATGCAAACG 
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC101472221 ( XM_004548515.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004548572.1 Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA.
    Price & Availability↑
    CloneID OMa22502
    Clone ID Related Accession (Same CDS sequence) XM_004548515.3 , XM_004548515.4
    Accession Version XM_004548515.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1524412800000
    Organism Maylandia zebra(zebra mbuna)
    Product voltage-dependent calcium channel subunit alpha-2/delta-2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004548515.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGATAG GCGAAAGGTC TTGCAGCATC GTCTGCTTGC TATCTATCCA GATTATCTTC 
    ATTTTCAGCG CATCGTGGCC TGGAGCGCAG GGTCTCTTCC CCCAGCAATA CACGATGATG 
    CACTGGGCCA GGCGTATCGA GCAGGAGATT GACAGAGTCT TTCAGCACAT CACTGGAGCT 
    CAGCAGTTGA AAGGGATATA CAATGAAGAA AGGCGTCGGT TTAGTCTGGT GAAGAATCAG 
    CCTCGCAAGA TTGTTGAAAA AGTGGCCTCG GATATTGAGA AACTTCTGGC TAAAAAGCGC 
    AAAGCACTTG ATAGGTTAGC CAGTGAAGCA GAGCGGCTCC AGCGGGAGCA TCTGTGGCAG 
    GATGAAATCA AGGAGCTGGA CATGGCTTAT TATGATTCCA AGGCAGAGCT GGATTATTAT 
    TCCATGGATG GAGAAGGAGA GGTGGAAAAT CCCTCTCACA TCAAGCTGGA GTTTGTGTAT 
    GACCCAAACT TCAAGAATAA TGTCAACTAT TCCTACACAG CTGTTCAGAT TCCCACGGAT 
    ATTTATAAAG GAGCTCCCGT CATTCTAAAC GAACTGAACT GGACGCAGGC GTTGGAGAAA 
    GTCTTCATGG AGAATAGTCG AGAGGATCCG TCACTGCTGT GGCAAGCATT TGGGAGCGCG 
    ACAGGAGTCA CTCGCTATTA TCCAGCAACA CCCTGGAAAG CTCCTGATAA AATTGACCTG 
    TATGATGTCA GGAGGAGGCC CTGGTACATC CAGGGTGCCT CATCCCCCAA AGACATGGTC 
    ATCCTTGTTG ACGTGAGTGG CAGTGTCAGT GGACTCACCC TGAAACTGAT CAAAGCGTCA 
    GTGATGGAAA TGCTGGACAC TTTATCCGAC GATGACTACG TCAATGTGGC CAGGTTTAAT 
    GAGAAGGCTG TGGCTGTGGT CCCTTGCTTT AGCCATCTAG TCCAGGCTAA TGTGCGCAAC 
    AAAAAGATTT TCAAGGATGC AGTGCAGCAG ATGCAGGCTA AAGGCACCAC TGACTACAAG 
    TCTGGATTTC ACTTTGCCTT CAATCAGCTA TTAAATAAAA CAAATGTCCC CCGGGCTAAC 
    TGCAATAAAA TAATCATGCT GTTTACTGAC GGAGGAGAGG ACCGAGCTCA GGATGTTTTC 
    ATGCAATACA ACTGGCCCAA CAAAACGGTT CGAGTATTTA CCTTTTCTGT GGGCCAGCAT 
    AACTATGATG TCACACCCTT ACAGTGGATT GCGTGCACCA ATAAAGGTTA CTATTTCGAG 
    ATCCGTTCCA TCTGCGCTAT AAGGATTAAC ACCCAGGAGT ACCTTGACGT GCTGGGACGC 
    CCCATGGTTC TGGCAGGCAG CGAAGCCAAG CAGGTTCAGT GGACCAATGT GTATCAGGAT 
    GCTCTGGGTC TTGGCATGGT AGTAACTGGG ACTTTGCCTG TTTTTAATCT CACCATGGAC 
    GGAAACTCAC AGAATCAGCT GATTTTAGGT GTCATGGGAG TCGACGTGCA TCTTGATGAG 
    ATAAAGCGAC TCACACCGCG GTACAATCTC GGGGCCAACG GATACATATT TGCCATCGAT 
    CCAAACGGAT ACCTGCTCCT TCATCCTAAT CTCCAGCCAA AGCTTGTGAA TCTGCCAGAA 
    CCTGTGACGC TGGACTTCCT GGACGCAGAG TTGGAGGACA GCAATAAAGA AGAGATCCGC 
    CGACAGATGA TCGATGGAAG ACCAGGTGAA ATGCAGATCA AAACTCTGGT ACTGTCGATT 
    GACGAGCGCT ACATCGATGA GGTCTACAGG GGTTACACCT GGACTCCTAT CAATGGAACA 
    GACTACAGTC TCGGTCTAGT ATTACCATCC TACAATGAGT ACTACATCAA GGCAGATCTG 
    AGCGATGTGA TGCTGCAGCT CCAGTATATG CAGTCTTTGC TGCCCAGCTC CTTTGAATCA 
    TCAGGGCACG TGTTTCTAGC TCCAAGGGAA TACTGCAAGC GTCTGCAGCT TTCTGACAAC 
    AACACCCAGT TCTTGCAGAA CTTTCTGTCT CTCATGCTTG ACCTCTCCCC TGAATCTGAT 
    GAGTGTGACC ATGGCCTGAT CCACAACCTG ATTTTTGATT CTAGGATCAT CGGGCAGCTT 
    GCTTCTCGTG TATGGAAGAA CAAGGACCTC AATTCGTATG GCTTCCTAGC TGTATTTGCA 
    TCCACAGACG GAGGAATAAC ACGAGTTTTT CCCAACATAG CTGCCGAGTT GTGGGATGAG 
    GACCCTGAAC CCTTCAATTC AAATTTCTAC AGACGGAGCC TCGACAACAA AGGCTACATG 
    TTCAGATCTA CACTCAGATC CTCTTTGGAT GACCCCATTG CAGCAGAAAA TGGGACGAGT 
    GAAATTGTGG TTAGTTCAGC TGTGGAGGTT AATTTAGCAG GAAAACTGCT CAGACCTGCA 
    GTGGTTGGTG GGAAGCTGGA CTTGGAAGCT TGGGTGGATA AGTTCAAAAT CCTGGCCAGC 
    AACATGTCAG ACGGCCGGCA GGGGTCACAC AAGTGTGGAC CTCTGAGAAG TTGCGAGATG 
    GATTGCGAGA TGGACACAAA TGAACTCGGC TGCTATCTCA TTGATGATGG TGGGTTCCTG 
    GTTATGTCCA ATCAGGCAGA TCACTTCCAA AAGATTGGTC TTTTCTTTGG CGATGTGGAC 
    GCTCCCCTGA TGTATGCCCT CTACAACAAT TCGATCTTCA CTCGACGGCA GTCTTTCCAG 
    TACCAGTCTG CATGTGAGCC GGTCAGCAGC AGCCACACCG GCGCAGCACC GAGAGGTGTT 
    TTTGTGCCGT CCATTGCTGA TGTTTTGAGC TTGGCATGGT GGACATCAAC AATGGCATGG 
    TCTGTGTTTC AGCAGCTTCT TTATGGACTG GCCTATAACA GCTGGCTCTA TCAAGCTGAT 
    GTCCTCATCG AAGGCTTTGA GACTAAAGAA AGCAGCTGTG TGACCATCCA AAGCCAGTTC 
    TACTTCACAA ACACCACAAA CTCCTACAAT ATGCTGCAGG ACTGTGGAAA CTGCTCCAGA 
    CTGTTCCATG CAAAGAGGAT CGAAAACACA AACCTGCTTT TTGTGGTCGC CGAGAAACTG 
    CTCTGCCAGT CGTGTGAGAT TGAGACACTG ACCCAGGTCA GAACAGAGTT TCAGGAGGAA 
    AATCCGTGTG AAGTATTGAA CAATACACGA TATCGTAAAG GCCCGACTTC CTGCTTTGAC 
    TACAGTGCCT TAGAAAACAC GTCAGAGTGT GGACGAGGTC ATTCCCTGCA GTCCTCCATA 
    GGAGTCCTCC TCTTCATTCA GCTCACTCTG CCTCTCTTTC ATCTCTCACA CATGCAAACG 
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004548572.1
    CDS322..3627
    Translation

    Target ORF information:

    RefSeq Version XM_004548515.4
    Organism Maylandia zebra(zebra mbuna)
    Definition Maylandia zebra voltage-dependent calcium channel subunit alpha-2/delta-2 (LOC101472221), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_004548515.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCGATAG GCGAAAGGTC TTGCAGCATC GTCTGCTTGC TATCTATCCA GATTATCTTC 
    ATTTTCAGCG CATCGTGGCC TGGAGCGCAG GGTCTCTTCC CCCAGCAATA CACGATGATG 
    CACTGGGCCA GGCGTATCGA GCAGGAGATT GACAGAGTCT TTCAGCACAT CACTGGAGCT 
    CAGCAGTTGA AAGGGATATA CAATGAAGAA AGGCGTCGGT TTAGTCTGGT GAAGAATCAG 
    CCTCGCAAGA TTGTTGAAAA AGTGGCCTCG GATATTGAGA AACTTCTGGC TAAAAAGCGC 
    AAAGCACTTG ATAGGTTAGC CAGTGAAGCA GAGCGGCTCC AGCGGGAGCA TCTGTGGCAG 
    GATGAAATCA AGGAGCTGGA CATGGCTTAT TATGATTCCA AGGCAGAGCT GGATTATTAT 
    TCCATGGATG GAGAAGGAGA GGTGGAAAAT CCCTCTCACA TCAAGCTGGA GTTTGTGTAT 
    GACCCAAACT TCAAGAATAA TGTCAACTAT TCCTACACAG CTGTTCAGAT TCCCACGGAT 
    ATTTATAAAG GAGCTCCCGT CATTCTAAAC GAACTGAACT GGACGCAGGC GTTGGAGAAA 
    GTCTTCATGG AGAATAGTCG AGAGGATCCG TCACTGCTGT GGCAAGCATT TGGGAGCGCG 
    ACAGGAGTCA CTCGCTATTA TCCAGCAACA CCCTGGAAAG CTCCTGATAA AATTGACCTG 
    TATGATGTCA GGAGGAGGCC CTGGTACATC CAGGGTGCCT CATCCCCCAA AGACATGGTC 
    ATCCTTGTTG ACGTGAGTGG CAGTGTCAGT GGACTCACCC TGAAACTGAT CAAAGCGTCA 
    GTGATGGAAA TGCTGGACAC TTTATCCGAC GATGACTACG TCAATGTGGC CAGGTTTAAT 
    GAGAAGGCTG TGGCTGTGGT CCCTTGCTTT AGCCATCTAG TCCAGGCTAA TGTGCGCAAC 
    AAAAAGATTT TCAAGGATGC AGTGCAGCAG ATGCAGGCTA AAGGCACCAC TGACTACAAG 
    TCTGGATTTC ACTTTGCCTT CAATCAGCTA TTAAATAAAA CAAATGTCCC CCGGGCTAAC 
    TGCAATAAAA TAATCATGCT GTTTACTGAC GGAGGAGAGG ACCGAGCTCA GGATGTTTTC 
    ATGCAATACA ACTGGCCCAA CAAAACGGTT CGAGTATTTA CCTTTTCTGT GGGCCAGCAT 
    AACTATGATG TCACACCCTT ACAGTGGATT GCGTGCACCA ATAAAGGTTA CTATTTCGAG 
    ATCCGTTCCA TCTGCGCTAT AAGGATTAAC ACCCAGGAGT ACCTTGACGT GCTGGGACGC 
    CCCATGGTTC TGGCAGGCAG CGAAGCCAAG CAGGTTCAGT GGACCAATGT GTATCAGGAT 
    GCTCTGGGTC TTGGCATGGT AGTAACTGGG ACTTTGCCTG TTTTTAATCT CACCATGGAC 
    GGAAACTCAC AGAATCAGCT GATTTTAGGT GTCATGGGAG TCGACGTGCA TCTTGATGAG 
    ATAAAGCGAC TCACACCGCG GTACAATCTC GGGGCCAACG GATACATATT TGCCATCGAT 
    CCAAACGGAT ACCTGCTCCT TCATCCTAAT CTCCAGCCAA AGCTTGTGAA TCTGCCAGAA 
    CCTGTGACGC TGGACTTCCT GGACGCAGAG TTGGAGGACA GCAATAAAGA AGAGATCCGC 
    CGACAGATGA TCGATGGAAG ACCAGGTGAA ATGCAGATCA AAACTCTGGT ACTGTCGATT 
    GACGAGCGCT ACATCGATGA GGTCTACAGG GGTTACACCT GGACTCCTAT CAATGGAACA 
    GACTACAGTC TCGGTCTAGT ATTACCATCC TACAATGAGT ACTACATCAA GGCAGATCTG 
    AGCGATGTGA TGCTGCAGCT CCAGTATATG CAGTCTTTGC TGCCCAGCTC CTTTGAATCA 
    TCAGGGCACG TGTTTCTAGC TCCAAGGGAA TACTGCAAGC GTCTGCAGCT TTCTGACAAC 
    AACACCCAGT TCTTGCAGAA CTTTCTGTCT CTCATGCTTG ACCTCTCCCC TGAATCTGAT 
    GAGTGTGACC ATGGCCTGAT CCACAACCTG ATTTTTGATT CTAGGATCAT CGGGCAGCTT 
    GCTTCTCGTG TATGGAAGAA CAAGGACCTC AATTCGTATG GCTTCCTAGC TGTATTTGCA 
    TCCACAGACG GAGGAATAAC ACGAGTTTTT CCCAACATAG CTGCCGAGTT GTGGGATGAG 
    GACCCTGAAC CCTTCAATTC AAATTTCTAC AGACGGAGCC TCGACAACAA AGGCTACATG 
    TTCAGATCTA CACTCAGATC CTCTTTGGAT GACCCCATTG CAGCAGAAAA TGGGACGAGT 
    GAAATTGTGG TTAGTTCAGC TGTGGAGGTT AATTTAGCAG GAAAACTGCT CAGACCTGCA 
    GTGGTTGGTG GGAAGCTGGA CTTGGAAGCT TGGGTGGATA AGTTCAAAAT CCTGGCCAGC 
    AACATGTCAG ACGGCCGGCA GGGGTCACAC AAGTGTGGAC CTCTGAGAAG TTGCGAGATG 
    GATTGCGAGA TGGACACAAA TGAACTCGGC TGCTATCTCA TTGATGATGG TGGGTTCCTG 
    GTTATGTCCA ATCAGGCAGA TCACTTCCAA AAGATTGGTC TTTTCTTTGG CGATGTGGAC 
    GCTCCCCTGA TGTATGCCCT CTACAACAAT TCGATCTTCA CTCGACGGCA GTCTTTCCAG 
    TACCAGTCTG CATGTGAGCC GGTCAGCAGC AGCCACACCG GCGCAGCACC GAGAGGTGTT 
    TTTGTGCCGT CCATTGCTGA TGTTTTGAGC TTGGCATGGT GGACATCAAC AATGGCATGG 
    TCTGTGTTTC AGCAGCTTCT TTATGGACTG GCCTATAACA GCTGGCTCTA TCAAGCTGAT 
    GTCCTCATCG AAGGCTTTGA GACTAAAGAA AGCAGCTGTG TGACCATCCA AAGCCAGTTC 
    TACTTCACAA ACACCACAAA CTCCTACAAT ATGCTGCAGG ACTGTGGAAA CTGCTCCAGA 
    CTGTTCCATG CAAAGAGGAT CGAAAACACA AACCTGCTTT TTGTGGTCGC CGAGAAACTG 
    CTCTGCCAGT CGTGTGAGAT TGAGACACTG ACCCAGGTCA GAACAGAGTT TCAGGAGGAA 
    AATCCGTGTG AAGTATTGAA CAATACACGA TATCGTAAAG GCCCGACTTC CTGCTTTGAC 
    TACAGTGCCT TAGAAAACAC GTCAGAGTGT GGACGAGGTC ATTCCCTGCA GTCCTCCATA 
    GGAGTCCTCC TCTTCATTCA GCTCACTCTG CCTCTCTTTC ATCTCTCACA CATGCAAACG 
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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