LOC101470954 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101470954
Entrez Gene ID	101470954
Full Name	laminin subunit beta-2
Synonyms	lamb2
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014409270.1	XP_014264756.1	laminin subunit beta-2 isoform X3
XM_014409269.1	XP_014264755.1	laminin subunit beta-2 isoform X2
XM_014409269.2	XP_014264755.2	laminin subunit beta-2 isoform X2
XM_014409268.1	XP_014264754.1	laminin subunit beta-2 isoform X1
XM_014409270.2	XP_014264756.2	laminin subunit beta-2 isoform X3
XM_014409268.2	XP_014264754.2	laminin subunit beta-2 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101470954 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101470954 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa06439	XM_014409268.1 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa06440	XM_014409269.1 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa06441	XM_014409270.1 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa368026	XM_014409268.2 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa368027	XM_014409269.2 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa368028	XM_014409270.2 Latest version!	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101470954 ( XM_014409268.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264754.1 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.

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CloneID	OMa06439
Clone ID Related Accession (Same CDS sequence)	XM_014409268.1
Accession Version	XM_014409268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTGT GTGTGTGTGT GTGTGAAGGA GCGAACAGAG CCAGCAGAAA GCTTCGGGGC 
AGGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCGGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCGCTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG AACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ATGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264754.1
CDS	149..5755
Translation	MCVCVCVCEGANRASRKLRGRQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCRNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIRYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRELRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409268.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409268.1

ORF Insert Sequence:

ATGTGTGTGT GTGTGTGTGT GTGTGAAGGA GCGAACAGAG CCAGCAGAAA GCTTCGGGGC 
AGGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCGGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCGCTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG AACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ATGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101470954 ( XM_014409269.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264755.1 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.

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CloneID	OMa06440
Clone ID Related Accession (Same CDS sequence)	XM_014409269.1
Accession Version	XM_014409269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC AAAGAGACAG CCTGTTGTCC ATCATGAATG TTCATCTGAG CCTCCTCATG 
CTGGCTGTCT GTGTATTTGG ACAAGAGCTC CCATCAAGCC CTCATGGCTG CACTGAGGGA 
AGCTGTTACC CTGCAACAGG AAACCTCCTG ATTGGACGTG CTGTCAATCT AACGGCCACC 
TCTACTTGCG GCCTTAATGG ACCAGAGCGA TATTGTATTG TCAGTCATCT GCAGCAACCA 
GAGAAGTGTT TTGAGTGCAA CTCCCAGCAT CCCTACGACC CGAACGTACA CTCGAACAGC 
CACCGCATTG AAAATGTGAT CTACCTCACA GACAGCAACA GAGTCAACAC CTGGTGGCAA 
TCTGTCCATG GCCAGGAGAG TGTCAGCATC AGACTGAACC TGGAGGCTGA ATTCCACTTT 
ACACATCTCA TCATGAAGTT TAAGACTTTC CGACCTGCAG CTCTGGTCAT TGAGCGTTCA 
GCTGACTTTG GCCGAACATG GCGTCCCTAC CGCTACTATG CCTCCAACTG TACCAGAACC 
TTCCCCAGCA TTCCTGCCAA TGGCTTGCAC CATATCAATG ACATCATCTG TGAGGAACGC 
TACTCTGACA TCGAACCCTC CACTAATGGA GAGGTTATTT ATAAAGTGCT GGATCCTGCC 
ATTCCAGTGA AAGACCCCTA CAGCTTAGAT ATTCAAGATC TTTTGCGCAT CACCAACCTG 
CGCATTAACT TCACAAAGCT CAACACTCTG GGAGATGACC TTCTGGACCG ACGCCCTGAA 
GTCCTGCAGA AATATTACTA CGCTATCTAT GAGCTGGTAG TGCGAGGGAG CTGCTTCTGT 
TACGGACATG CTTCAGAGTG CGCTCCTGTG CCGGGGGTCA ACGCCAGGGA GATCGGCATG 
ATCCACGGTC GCTGTGTGTG CAAACACAAC ACAGAAGGCC TGAACTGCGA GCGCTGCCGG 
AACTTCCACC ATGACCTGCC GTGGAGACCA GCGGAGGCCG AGAACCCTCA CACCTGCAGA 
GAGTGTAACT GTAATGGCCA CTCGGACCAC TGCCACTTTG ACATGGCAGT GTATATGGCA 
ACGGGTAATG TTACTGGAGG AGTTTGTGAT GACTGTCTGC ACAACACTAT GGGACGCAAC 
TGTGAGACGT GTAAACCTTT CTACTACCAA GATCCAAACA GAGATGTTAG AGATCCACGG 
GTTTGTATCT CCTGTGACTG TGACCCAGTG GGGTCATTGG AGGGAGGCGT GTGTGACACC 
CACACCAACT TTGAAATGGG AATGATTGCG GGACAGTGTC GCTGCAAAGT CAACGTGAAG 
GGGACGCGCT GTGATGACTG CAAGGAGGGT TATTATGGAC TCAGCCAGAA TGACCCTTTG 
GGTTGCCAAC CTTGCAACTG TGATCCTCGT GGCATCATCG TGATGGGAGC TCCTTGTGAT 
CAGATCAGTG GGGACTGCTC CTGTAAAAGA TATGTCACCG GCCGCTACTG CAACCAGTGT 
CTGCCTGAAT ACTGGGGCCT TAGTAATGAC CTGGCAGGCT GCAGACCTTG TGACTGTGAC 
TTTGGAGGAG CCTTCAACAA CAGGTGCATG ATGGAAACCG GCCAGTGTGA CTGCAGGAGG 
CACCTAATTG GCCGGCAATG CTCTGATGTG CAGCCCGGGT ATTTCTGCGC TCCGCTGGAC 
TTCTACAAGT ATGAGGCCGA AGATGCCACC GGACACTCTC CTGCTGACCC CGTACTGCCG 
GGCCAAGTTC GCCAACAAGC AACTAATGAC TGTGTTCAGC ACCTCAGCAA CCAGCTCAGG 
AGGCACCGGC GGCACCGTAA CATTGCCAAG TCGCAGCAAC ATCAGGCAGC ACTGAGACGT 
ATTCGGCAGC TTCAACAAAC ACCGGATGTG AGGACCGTTC ATAGAGAGCA CACTCTCAGC 
CATATGGTGA CATGGACAGG ACCTGGTTTT TCCCGTGTTA AAGATGGCGC TGGTCTGGTT 
TTCACCATTG ATAACATACC TTATCCCATG GAGTATGACA TCATGATCCG CTATGAACCT 
GAGTCTACAG AGAACTGGGA GGCTGTTGTT AGTATTACAT CTGTAGAATT GCCCTCCAGT 
CTCCGGTGTG GAAACCTCCT ACCTACTGAA CAGCTCTACA CTGTCACTTT GCCACACCAC 
AACAGATACA TCCAGATGCC CAGACCATTT TGTTTTGAGC CCAGCAATCG TTACGTGGTT 
ACAATCAGGT TCCAGCGTCA TGGCGCCACA CACAGACATC TCACTGCCTT CATTCTTATT 
GACTCGCTGG TATTGATCCC TAAATACATG GAGCTTCCTG GGTTTCAAGG TAACACACCA 
GAGTCAGAGC AGCGTCAGGA GGAGATGATC CGCTACATGT GTCTTGATTC CTTTATGATC 
ACTCCGATGC CTGCACTGGC TGAGATGTGC TCTAAACTCA TCTGCAGTAT TTCTTCCATT 
ATTCACGACG GTGCCCTGCC CTGTGAGTGT GACCCTCAGG GCTCCCTCAG CGGTGAGTGT 
GACGTAGTGG GAGGTCAGTG CCGTTGTAAA CCCAACGTGA TTGGCAGGCG CTGTGACCAG 
TGTGCCCCGG GGACCTACGG CTTCGGTGTG AGCGGCTGCA CTGCCTGTGA CTGCCACTCC 
GACGGCTCAT TGAGCCACCA GTGTGACCCA GTTACAGGCC AGTGTCAGTG CAGACAAGGA 
GCCACAGGGC GGCAGTGCTC TGACTGTCAG CCGGGCCAGT GGGGCTTCCC CAGCTGCACC 
CCCTGTCAGT GTAACGGCCA TGCAGACCTC TGCGATCCCC GCACGGGAGA ATGCCGAGGC 
TGCAGGGACT TCACAGCAGG ACATCTCTGT GAGCGTTGCA TGGACACGTT CTTTGGAAAC 
CCAGCACTTG GATCAGGGCA GCACTGCCGG CCCTGCCCCT GCCCAAATAA CCCCAACTCC 
GATAACTTCA ATGCGTTATC TTGTCAGGTT GACCACACCT CTGATCAGAT CATGTGTAAC 
TGCAAACAGG GCTACATGGG TCTTCGGTGT GACCAGTGTG CCCCTGGTTA CTATGGTAAC 
CCTGAGCAAC CTGGCGGACA GTGCTTTCCA TGTGAGTGCA ACGGCAACAT AGACACCCAG 
GATCCTGAGT CATGTGATCC CAGGACTGGC GAGTGCATCA AGTGCCTGTA CCACACTGAC 
GGCCCGTCTT GCGCCCAGTG TCAAATCGGT TACTATGGCA ACGCTTTGGC CCACGATTGC 
AGACGCTGTA CCTGTGTGAC TGCTGGTACC CTACAGTCTG CTTGCAGCGA TGGACACTGT 
GACTGTGATC GGCAGGCTGG AGCCTGTCTC TGCAGGGAGC ATGTGGTAGG CCATAACTGT 
GACCAGTGTG CACCTAACTA CTGGAACTTC GGTCAGGATC AGGGCTGTGA GCCGTGTAAT 
TGCAACTCGC AGCACGCTCT GGGAACTCAC TGCAACATGT TCACAGGCCA GTGTCACTGT 
CATTCAGGCT TTGGAGGTAA ACAATGCACC GAGTGTGAGC TGTTCCACTG GGGAGATCCA 
CGGGAGCAGT GCCACGAATG CAACTGTAAC CCAGAGGGCA CAGAGATTGC CCAGTGTGAC 
AGATTAACGG GTGCGTGTGA ATGCCGGGAA GGAGCCGCGG GACTGCAGTG CAACCAGTGC 
GCTCGTGGTT TCACCGGCAT CTTCCCAAAA TGCATCCAGT GTCACCCGTG CTTCCAGCTC 
TGGGATGACG CCCTGTGCCA GATTAGAAGG GACCTGGATC ACATCCAGTC CAGAGTGCAG 
GAGATCCTGA AGGGTGGCAT CACACCGGGA GCTGGGGACG CACGCATCAA AGAACTGGAA 
GGGAAGCTGA AGCAGGTGAA GGATCTGATC ATTACGCAGG ACAGGGACAG GGTCTACCAG 
CTCATTGGCC AGAGCATCGA TGACCTCAGG GCTGAGATTG CCCTCACTGA TGGGCGTCTG 
ATGGCCATGG GCCAAGAGCT GAATACGACA GCAGAAACAG ACAGGGAACT CCGACACACG 
CTGGATAACA TGGAAAAAGA GCTGCGGGAC CTCAACACCA CTGTGACTGA CAAACAGAAG 
CTGCTTGAAC TGTTCGGCTC AGGGTTTGCA GATCAGTTTG AGAAAGTGAA GCAGTACTAT 
CAGGAATCGC TGCAGGCTGA GATGAAATGC AACGCTTCAG TGTTTGGTCC TATGAGTCCT 
GTGGAAGAGT CAAATAAGAC CAGAGCGCGC ACAGAAGACC TGCTGGATGA AATTGAAAAC 
AGCATTAACC GTAATGTGGC TGCTCACAAC AAGTCACTCA GCGACCTACA AAAACAAGCT 
CACGACCTCC ATAAACAGGT CAATCAGCTG AGCGACCAGG TGTGTGGCGG TCACAGCAAT 
AACAGCGTCA ATGGCAGTTG CCCAGACAGT CAGTGTGGTG GTGCCGGCTG CCATGACAAT 
CAGGGAAACC ATGTATGTGG AGGAGTGGGA TGCAGCGGGA CTGTGGGCAC ATCTGTAGCT 
GCGCTGAATG ACACCAGGAA CGTTACAGAC AGTCTGAACA CTGCCAGTAA GGAGCTGCAG 
AAAGTCTCCA AAAAGCTCCA GGACATAGCT GCTATGACAC AAAAACTGAA GAACCAGACG 
ATAAACACGC TGGAAAAAGC CAACAAGTTG AGCAAGGAGC TTAAAGAAAG CAATAAGAAC 
CTGACAGAGT TCATTGAGAA AATTAAAAAC TTTCTCACTG AGGAGGGAGC CGACCCAGAG 
AGCATAGAGA AAGTGGCTCA GGAGGTCTTG TCGATCACAC TGCCACTGAA CAGGACCGTT 
CTGGACAAAA TGATCGCGCA GATAAAGGAA AGTCTTTCCA ACATCAGCAA TATTGAGGGG 
ATTGTAAATC AAACCACACT GCACACCACT AAAGTCAAGG AGCTTCTTGA TGCAGCTAAA 
GAGGCCAAGA GGCGAGCAGA GGAAGTGAAA GACATGACCA ATATTACCAA GGAGGCGTTA 
AAAGCATCTG GAAAGGCCAT AGAGAAAGCA CAGGCTGCCC TGAAAGAAGC CCACAACAAC 
CTGAACAGCA CAAGAAATGC TACTGCTGAG GTGGAGGGAA AGCTGGTGAG CCTAGACGCC 
AAGCAGATGG ATGTCATGAT GCGTTTAATG AACCTCTCCA AGGAGGTGGA AGCTCTGAAG 
AACAAGACGG AGCAGAACAG ACAGATGGCA ATCGAGGCTA AAGCACTGGC CGACAATGCC 
ACTGACCTAA CATCTTCACT GGAGCAGAGC CTCAACAACA CAGAGAAACA GTATCGTGAG 
CTGCAGATGA AGATCGAGTC TCTTGGTGGG GTGTCTGGAG GTCTGAAGGA CATCAACCAG 
AGGGTCAAAG ATCTCAAAGA GGATGCAGAG AAATGGCTCA AGAATGCCAC CAGTGGGATA 
AGAGAACTGG ACGAACTTGA GGAAAAGTTC AAAAACAATG AGAGGCGGAT GGAGAAGCAG 
CGCTTGGAGT TGGACAAGCT GAAAGACAAC GCAACTAGTG TGCGGGACGA GATCCGGCAT 
CGAGTGCAGA ACTACAACAC CTGTGTGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264755.1
CDS	193..5745
Translation	MAVQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCRNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIRYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRELRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409269.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409269.1

ORF Insert Sequence:

ATGGCTGTGC AAAGAGACAG CCTGTTGTCC ATCATGAATG TTCATCTGAG CCTCCTCATG 
CTGGCTGTCT GTGTATTTGG ACAAGAGCTC CCATCAAGCC CTCATGGCTG CACTGAGGGA 
AGCTGTTACC CTGCAACAGG AAACCTCCTG ATTGGACGTG CTGTCAATCT AACGGCCACC 
TCTACTTGCG GCCTTAATGG ACCAGAGCGA TATTGTATTG TCAGTCATCT GCAGCAACCA 
GAGAAGTGTT TTGAGTGCAA CTCCCAGCAT CCCTACGACC CGAACGTACA CTCGAACAGC 
CACCGCATTG AAAATGTGAT CTACCTCACA GACAGCAACA GAGTCAACAC CTGGTGGCAA 
TCTGTCCATG GCCAGGAGAG TGTCAGCATC AGACTGAACC TGGAGGCTGA ATTCCACTTT 
ACACATCTCA TCATGAAGTT TAAGACTTTC CGACCTGCAG CTCTGGTCAT TGAGCGTTCA 
GCTGACTTTG GCCGAACATG GCGTCCCTAC CGCTACTATG CCTCCAACTG TACCAGAACC 
TTCCCCAGCA TTCCTGCCAA TGGCTTGCAC CATATCAATG ACATCATCTG TGAGGAACGC 
TACTCTGACA TCGAACCCTC CACTAATGGA GAGGTTATTT ATAAAGTGCT GGATCCTGCC 
ATTCCAGTGA AAGACCCCTA CAGCTTAGAT ATTCAAGATC TTTTGCGCAT CACCAACCTG 
CGCATTAACT TCACAAAGCT CAACACTCTG GGAGATGACC TTCTGGACCG ACGCCCTGAA 
GTCCTGCAGA AATATTACTA CGCTATCTAT GAGCTGGTAG TGCGAGGGAG CTGCTTCTGT 
TACGGACATG CTTCAGAGTG CGCTCCTGTG CCGGGGGTCA ACGCCAGGGA GATCGGCATG 
ATCCACGGTC GCTGTGTGTG CAAACACAAC ACAGAAGGCC TGAACTGCGA GCGCTGCCGG 
AACTTCCACC ATGACCTGCC GTGGAGACCA GCGGAGGCCG AGAACCCTCA CACCTGCAGA 
GAGTGTAACT GTAATGGCCA CTCGGACCAC TGCCACTTTG ACATGGCAGT GTATATGGCA 
ACGGGTAATG TTACTGGAGG AGTTTGTGAT GACTGTCTGC ACAACACTAT GGGACGCAAC 
TGTGAGACGT GTAAACCTTT CTACTACCAA GATCCAAACA GAGATGTTAG AGATCCACGG 
GTTTGTATCT CCTGTGACTG TGACCCAGTG GGGTCATTGG AGGGAGGCGT GTGTGACACC 
CACACCAACT TTGAAATGGG AATGATTGCG GGACAGTGTC GCTGCAAAGT CAACGTGAAG 
GGGACGCGCT GTGATGACTG CAAGGAGGGT TATTATGGAC TCAGCCAGAA TGACCCTTTG 
GGTTGCCAAC CTTGCAACTG TGATCCTCGT GGCATCATCG TGATGGGAGC TCCTTGTGAT 
CAGATCAGTG GGGACTGCTC CTGTAAAAGA TATGTCACCG GCCGCTACTG CAACCAGTGT 
CTGCCTGAAT ACTGGGGCCT TAGTAATGAC CTGGCAGGCT GCAGACCTTG TGACTGTGAC 
TTTGGAGGAG CCTTCAACAA CAGGTGCATG ATGGAAACCG GCCAGTGTGA CTGCAGGAGG 
CACCTAATTG GCCGGCAATG CTCTGATGTG CAGCCCGGGT ATTTCTGCGC TCCGCTGGAC 
TTCTACAAGT ATGAGGCCGA AGATGCCACC GGACACTCTC CTGCTGACCC CGTACTGCCG 
GGCCAAGTTC GCCAACAAGC AACTAATGAC TGTGTTCAGC ACCTCAGCAA CCAGCTCAGG 
AGGCACCGGC GGCACCGTAA CATTGCCAAG TCGCAGCAAC ATCAGGCAGC ACTGAGACGT 
ATTCGGCAGC TTCAACAAAC ACCGGATGTG AGGACCGTTC ATAGAGAGCA CACTCTCAGC 
CATATGGTGA CATGGACAGG ACCTGGTTTT TCCCGTGTTA AAGATGGCGC TGGTCTGGTT 
TTCACCATTG ATAACATACC TTATCCCATG GAGTATGACA TCATGATCCG CTATGAACCT 
GAGTCTACAG AGAACTGGGA GGCTGTTGTT AGTATTACAT CTGTAGAATT GCCCTCCAGT 
CTCCGGTGTG GAAACCTCCT ACCTACTGAA CAGCTCTACA CTGTCACTTT GCCACACCAC 
AACAGATACA TCCAGATGCC CAGACCATTT TGTTTTGAGC CCAGCAATCG TTACGTGGTT 
ACAATCAGGT TCCAGCGTCA TGGCGCCACA CACAGACATC TCACTGCCTT CATTCTTATT 
GACTCGCTGG TATTGATCCC TAAATACATG GAGCTTCCTG GGTTTCAAGG TAACACACCA 
GAGTCAGAGC AGCGTCAGGA GGAGATGATC CGCTACATGT GTCTTGATTC CTTTATGATC 
ACTCCGATGC CTGCACTGGC TGAGATGTGC TCTAAACTCA TCTGCAGTAT TTCTTCCATT 
ATTCACGACG GTGCCCTGCC CTGTGAGTGT GACCCTCAGG GCTCCCTCAG CGGTGAGTGT 
GACGTAGTGG GAGGTCAGTG CCGTTGTAAA CCCAACGTGA TTGGCAGGCG CTGTGACCAG 
TGTGCCCCGG GGACCTACGG CTTCGGTGTG AGCGGCTGCA CTGCCTGTGA CTGCCACTCC 
GACGGCTCAT TGAGCCACCA GTGTGACCCA GTTACAGGCC AGTGTCAGTG CAGACAAGGA 
GCCACAGGGC GGCAGTGCTC TGACTGTCAG CCGGGCCAGT GGGGCTTCCC CAGCTGCACC 
CCCTGTCAGT GTAACGGCCA TGCAGACCTC TGCGATCCCC GCACGGGAGA ATGCCGAGGC 
TGCAGGGACT TCACAGCAGG ACATCTCTGT GAGCGTTGCA TGGACACGTT CTTTGGAAAC 
CCAGCACTTG GATCAGGGCA GCACTGCCGG CCCTGCCCCT GCCCAAATAA CCCCAACTCC 
GATAACTTCA ATGCGTTATC TTGTCAGGTT GACCACACCT CTGATCAGAT CATGTGTAAC 
TGCAAACAGG GCTACATGGG TCTTCGGTGT GACCAGTGTG CCCCTGGTTA CTATGGTAAC 
CCTGAGCAAC CTGGCGGACA GTGCTTTCCA TGTGAGTGCA ACGGCAACAT AGACACCCAG 
GATCCTGAGT CATGTGATCC CAGGACTGGC GAGTGCATCA AGTGCCTGTA CCACACTGAC 
GGCCCGTCTT GCGCCCAGTG TCAAATCGGT TACTATGGCA ACGCTTTGGC CCACGATTGC 
AGACGCTGTA CCTGTGTGAC TGCTGGTACC CTACAGTCTG CTTGCAGCGA TGGACACTGT 
GACTGTGATC GGCAGGCTGG AGCCTGTCTC TGCAGGGAGC ATGTGGTAGG CCATAACTGT 
GACCAGTGTG CACCTAACTA CTGGAACTTC GGTCAGGATC AGGGCTGTGA GCCGTGTAAT 
TGCAACTCGC AGCACGCTCT GGGAACTCAC TGCAACATGT TCACAGGCCA GTGTCACTGT 
CATTCAGGCT TTGGAGGTAA ACAATGCACC GAGTGTGAGC TGTTCCACTG GGGAGATCCA 
CGGGAGCAGT GCCACGAATG CAACTGTAAC CCAGAGGGCA CAGAGATTGC CCAGTGTGAC 
AGATTAACGG GTGCGTGTGA ATGCCGGGAA GGAGCCGCGG GACTGCAGTG CAACCAGTGC 
GCTCGTGGTT TCACCGGCAT CTTCCCAAAA TGCATCCAGT GTCACCCGTG CTTCCAGCTC 
TGGGATGACG CCCTGTGCCA GATTAGAAGG GACCTGGATC ACATCCAGTC CAGAGTGCAG 
GAGATCCTGA AGGGTGGCAT CACACCGGGA GCTGGGGACG CACGCATCAA AGAACTGGAA 
GGGAAGCTGA AGCAGGTGAA GGATCTGATC ATTACGCAGG ACAGGGACAG GGTCTACCAG 
CTCATTGGCC AGAGCATCGA TGACCTCAGG GCTGAGATTG CCCTCACTGA TGGGCGTCTG 
ATGGCCATGG GCCAAGAGCT GAATACGACA GCAGAAACAG ACAGGGAACT CCGACACACG 
CTGGATAACA TGGAAAAAGA GCTGCGGGAC CTCAACACCA CTGTGACTGA CAAACAGAAG 
CTGCTTGAAC TGTTCGGCTC AGGGTTTGCA GATCAGTTTG AGAAAGTGAA GCAGTACTAT 
CAGGAATCGC TGCAGGCTGA GATGAAATGC AACGCTTCAG TGTTTGGTCC TATGAGTCCT 
GTGGAAGAGT CAAATAAGAC CAGAGCGCGC ACAGAAGACC TGCTGGATGA AATTGAAAAC 
AGCATTAACC GTAATGTGGC TGCTCACAAC AAGTCACTCA GCGACCTACA AAAACAAGCT 
CACGACCTCC ATAAACAGGT CAATCAGCTG AGCGACCAGG TGTGTGGCGG TCACAGCAAT 
AACAGCGTCA ATGGCAGTTG CCCAGACAGT CAGTGTGGTG GTGCCGGCTG CCATGACAAT 
CAGGGAAACC ATGTATGTGG AGGAGTGGGA TGCAGCGGGA CTGTGGGCAC ATCTGTAGCT 
GCGCTGAATG ACACCAGGAA CGTTACAGAC AGTCTGAACA CTGCCAGTAA GGAGCTGCAG 
AAAGTCTCCA AAAAGCTCCA GGACATAGCT GCTATGACAC AAAAACTGAA GAACCAGACG 
ATAAACACGC TGGAAAAAGC CAACAAGTTG AGCAAGGAGC TTAAAGAAAG CAATAAGAAC 
CTGACAGAGT TCATTGAGAA AATTAAAAAC TTTCTCACTG AGGAGGGAGC CGACCCAGAG 
AGCATAGAGA AAGTGGCTCA GGAGGTCTTG TCGATCACAC TGCCACTGAA CAGGACCGTT 
CTGGACAAAA TGATCGCGCA GATAAAGGAA AGTCTTTCCA ACATCAGCAA TATTGAGGGG 
ATTGTAAATC AAACCACACT GCACACCACT AAAGTCAAGG AGCTTCTTGA TGCAGCTAAA 
GAGGCCAAGA GGCGAGCAGA GGAAGTGAAA GACATGACCA ATATTACCAA GGAGGCGTTA 
AAAGCATCTG GAAAGGCCAT AGAGAAAGCA CAGGCTGCCC TGAAAGAAGC CCACAACAAC 
CTGAACAGCA CAAGAAATGC TACTGCTGAG GTGGAGGGAA AGCTGGTGAG CCTAGACGCC 
AAGCAGATGG ATGTCATGAT GCGTTTAATG AACCTCTCCA AGGAGGTGGA AGCTCTGAAG 
AACAAGACGG AGCAGAACAG ACAGATGGCA ATCGAGGCTA AAGCACTGGC CGACAATGCC 
ACTGACCTAA CATCTTCACT GGAGCAGAGC CTCAACAACA CAGAGAAACA GTATCGTGAG 
CTGCAGATGA AGATCGAGTC TCTTGGTGGG GTGTCTGGAG GTCTGAAGGA CATCAACCAG 
AGGGTCAAAG ATCTCAAAGA GGATGCAGAG AAATGGCTCA AGAATGCCAC CAGTGGGATA 
AGAGAACTGG ACGAACTTGA GGAAAAGTTC AAAAACAATG AGAGGCGGAT GGAGAAGCAG 
CGCTTGGAGT TGGACAAGCT GAAAGACAAC GCAACTAGTG TGCGGGACGA GATCCGGCAT 
CGAGTGCAGA ACTACAACAC CTGTGTGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101470954 ( XM_014409270.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264756.1 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.

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CloneID	OMa06441
Clone ID Related Accession (Same CDS sequence)	XM_014409270.1
Accession Version	XM_014409270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCGGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCGCTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG AACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ATGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264756.1
CDS	69..5615
Translation	MQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCRNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIRYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRELRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409270.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409270.1

ORF Insert Sequence:

ATGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCGGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCGCTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG AACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ATGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101470954 ( XM_014409268.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264754.2 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.

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CloneID	OMa368026
Clone ID Related Accession (Same CDS sequence)	XM_014409268.2
Accession Version	XM_014409268.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036799.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014409268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTGT GTGTGTGTGT GTGTGAAGGA GCGAACAGAG CCAGCAGAAA GCTTCGGGGC 
AGGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCAGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCACTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG CACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ACGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264754.2
CDS	149..5755
Translation	MCVCVCVCEGANRASRKLRGRQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCQNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIHYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRALRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409268.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409268.2

ORF Insert Sequence:

ATGTGTGTGT GTGTGTGTGT GTGTGAAGGA GCGAACAGAG CCAGCAGAAA GCTTCGGGGC 
AGGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCAGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCACTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG CACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ACGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101470954 ( XM_014409269.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264755.2 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.

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CloneID	OMa368027
Clone ID Related Accession (Same CDS sequence)	XM_014409269.2
Accession Version	XM_014409269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036799.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014409269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGC AAAGAGACAG CCTGTTGTCC ATCATGAATG TTCATCTGAG CCTCCTCATG 
CTGGCTGTCT GTGTATTTGG ACAAGAGCTC CCATCAAGCC CTCATGGCTG CACTGAGGGA 
AGCTGTTACC CTGCAACAGG AAACCTCCTG ATTGGACGTG CTGTCAATCT AACGGCCACC 
TCTACTTGCG GCCTTAATGG ACCAGAGCGA TATTGTATTG TCAGTCATCT GCAGCAACCA 
GAGAAGTGTT TTGAGTGCAA CTCCCAGCAT CCCTACGACC CGAACGTACA CTCGAACAGC 
CACCGCATTG AAAATGTGAT CTACCTCACA GACAGCAACA GAGTCAACAC CTGGTGGCAA 
TCTGTCCATG GCCAGGAGAG TGTCAGCATC AGACTGAACC TGGAGGCTGA ATTCCACTTT 
ACACATCTCA TCATGAAGTT TAAGACTTTC CGACCTGCAG CTCTGGTCAT TGAGCGTTCA 
GCTGACTTTG GCCGAACATG GCGTCCCTAC CGCTACTATG CCTCCAACTG TACCAGAACC 
TTCCCCAGCA TTCCTGCCAA TGGCTTGCAC CATATCAATG ACATCATCTG TGAGGAACGC 
TACTCTGACA TCGAACCCTC CACTAATGGA GAGGTTATTT ATAAAGTGCT GGATCCTGCC 
ATTCCAGTGA AAGACCCCTA CAGCTTAGAT ATTCAAGATC TTTTGCGCAT CACCAACCTG 
CGCATTAACT TCACAAAGCT CAACACTCTG GGAGATGACC TTCTGGACCG ACGCCCTGAA 
GTCCTGCAGA AATATTACTA CGCTATCTAT GAGCTGGTAG TGCGAGGGAG CTGCTTCTGT 
TACGGACATG CTTCAGAGTG CGCTCCTGTG CCGGGGGTCA ACGCCAGGGA GATCGGCATG 
ATCCACGGTC GCTGTGTGTG CAAACACAAC ACAGAAGGCC TGAACTGCGA GCGCTGCCAG 
AACTTCCACC ATGACCTGCC GTGGAGACCA GCGGAGGCCG AGAACCCTCA CACCTGCAGA 
GAGTGTAACT GTAATGGCCA CTCGGACCAC TGCCACTTTG ACATGGCAGT GTATATGGCA 
ACGGGTAATG TTACTGGAGG AGTTTGTGAT GACTGTCTGC ACAACACTAT GGGACGCAAC 
TGTGAGACGT GTAAACCTTT CTACTACCAA GATCCAAACA GAGATGTTAG AGATCCACGG 
GTTTGTATCT CCTGTGACTG TGACCCAGTG GGGTCATTGG AGGGAGGCGT GTGTGACACC 
CACACCAACT TTGAAATGGG AATGATTGCG GGACAGTGTC GCTGCAAAGT CAACGTGAAG 
GGGACGCGCT GTGATGACTG CAAGGAGGGT TATTATGGAC TCAGCCAGAA TGACCCTTTG 
GGTTGCCAAC CTTGCAACTG TGATCCTCGT GGCATCATCG TGATGGGAGC TCCTTGTGAT 
CAGATCAGTG GGGACTGCTC CTGTAAAAGA TATGTCACCG GCCGCTACTG CAACCAGTGT 
CTGCCTGAAT ACTGGGGCCT TAGTAATGAC CTGGCAGGCT GCAGACCTTG TGACTGTGAC 
TTTGGAGGAG CCTTCAACAA CAGGTGCATG ATGGAAACCG GCCAGTGTGA CTGCAGGAGG 
CACCTAATTG GCCGGCAATG CTCTGATGTG CAGCCCGGGT ATTTCTGCGC TCCGCTGGAC 
TTCTACAAGT ATGAGGCCGA AGATGCCACC GGACACTCTC CTGCTGACCC CGTACTGCCG 
GGCCAAGTTC GCCAACAAGC AACTAATGAC TGTGTTCAGC ACCTCAGCAA CCAGCTCAGG 
AGGCACCGGC GGCACCGTAA CATTGCCAAG TCGCAGCAAC ATCAGGCAGC ACTGAGACGT 
ATTCGGCAGC TTCAACAAAC ACCGGATGTG AGGACCGTTC ATAGAGAGCA CACTCTCAGC 
CATATGGTGA CATGGACAGG ACCTGGTTTT TCCCGTGTTA AAGATGGCGC TGGTCTGGTT 
TTCACCATTG ATAACATACC TTATCCCATG GAGTATGACA TCATGATCCG CTATGAACCT 
GAGTCTACAG AGAACTGGGA GGCTGTTGTT AGTATTACAT CTGTAGAATT GCCCTCCAGT 
CTCCGGTGTG GAAACCTCCT ACCTACTGAA CAGCTCTACA CTGTCACTTT GCCACACCAC 
AACAGATACA TCCAGATGCC CAGACCATTT TGTTTTGAGC CCAGCAATCG TTACGTGGTT 
ACAATCAGGT TCCAGCGTCA TGGCGCCACA CACAGACATC TCACTGCCTT CATTCTTATT 
GACTCGCTGG TATTGATCCC TAAATACATG GAGCTTCCTG GGTTTCAAGG TAACACACCA 
GAGTCAGAGC AGCGTCAGGA GGAGATGATC CACTACATGT GTCTTGATTC CTTTATGATC 
ACTCCGATGC CTGCACTGGC TGAGATGTGC TCTAAACTCA TCTGCAGTAT TTCTTCCATT 
ATTCACGACG GTGCCCTGCC CTGTGAGTGT GACCCTCAGG GCTCCCTCAG CGGTGAGTGT 
GACGTAGTGG GAGGTCAGTG CCGTTGTAAA CCCAACGTGA TTGGCAGGCG CTGTGACCAG 
TGTGCCCCGG GGACCTACGG CTTCGGTGTG AGCGGCTGCA CTGCCTGTGA CTGCCACTCC 
GACGGCTCAT TGAGCCACCA GTGTGACCCA GTTACAGGCC AGTGTCAGTG CAGACAAGGA 
GCCACAGGGC GGCAGTGCTC TGACTGTCAG CCGGGCCAGT GGGGCTTCCC CAGCTGCACC 
CCCTGTCAGT GTAACGGCCA TGCAGACCTC TGCGATCCCC GCACGGGAGA ATGCCGAGGC 
TGCAGGGACT TCACAGCAGG ACATCTCTGT GAGCGTTGCA TGGACACGTT CTTTGGAAAC 
CCAGCACTTG GATCAGGGCA GCACTGCCGG CCCTGCCCCT GCCCAAATAA CCCCAACTCC 
GATAACTTCA ATGCGTTATC TTGTCAGGTT GACCACACCT CTGATCAGAT CATGTGTAAC 
TGCAAACAGG GCTACATGGG TCTTCGGTGT GACCAGTGTG CCCCTGGTTA CTATGGTAAC 
CCTGAGCAAC CTGGCGGACA GTGCTTTCCA TGTGAGTGCA ACGGCAACAT AGACACCCAG 
GATCCTGAGT CATGTGATCC CAGGACTGGC GAGTGCATCA AGTGCCTGTA CCACACTGAC 
GGCCCGTCTT GCGCCCAGTG TCAAATCGGT TACTATGGCA ACGCTTTGGC CCACGATTGC 
AGACGCTGTA CCTGTGTGAC TGCTGGTACC CTACAGTCTG CTTGCAGCGA TGGACACTGT 
GACTGTGATC GGCAGGCTGG AGCCTGTCTC TGCAGGGAGC ATGTGGTAGG CCATAACTGT 
GACCAGTGTG CACCTAACTA CTGGAACTTC GGTCAGGATC AGGGCTGTGA GCCGTGTAAT 
TGCAACTCGC AGCACGCTCT GGGAACTCAC TGCAACATGT TCACAGGCCA GTGTCACTGT 
CATTCAGGCT TTGGAGGTAA ACAATGCACC GAGTGTGAGC TGTTCCACTG GGGAGATCCA 
CGGGAGCAGT GCCACGAATG CAACTGTAAC CCAGAGGGCA CAGAGATTGC CCAGTGTGAC 
AGATTAACGG GTGCGTGTGA ATGCCGGGAA GGAGCCGCGG GACTGCAGTG CAACCAGTGC 
GCTCGTGGTT TCACCGGCAT CTTCCCAAAA TGCATCCAGT GTCACCCGTG CTTCCAGCTC 
TGGGATGACG CCCTGTGCCA GATTAGAAGG GACCTGGATC ACATCCAGTC CAGAGTGCAG 
GAGATCCTGA AGGGTGGCAT CACACCGGGA GCTGGGGACG CACGCATCAA AGAACTGGAA 
GGGAAGCTGA AGCAGGTGAA GGATCTGATC ATTACGCAGG ACAGGGACAG GGTCTACCAG 
CTCATTGGCC AGAGCATCGA TGACCTCAGG GCTGAGATTG CCCTCACTGA TGGGCGTCTG 
ATGGCCATGG GCCAAGAGCT GAATACGACA GCAGAAACAG ACAGGGCACT CCGACACACG 
CTGGATAACA TGGAAAAAGA GCTGCGGGAC CTCAACACCA CTGTGACTGA CAAACAGAAG 
CTGCTTGAAC TGTTCGGCTC AGGGTTTGCA GATCAGTTTG AGAAAGTGAA GCAGTACTAT 
CAGGAATCGC TGCAGGCTGA GATGAAATGC AACGCTTCAG TGTTTGGTCC TATGAGTCCT 
GTGGAAGAGT CAAATAAGAC CAGAGCGCGC ACAGAAGACC TGCTGGATGA AATTGAAAAC 
AGCATTAACC GTAATGTGGC TGCTCACAAC AAGTCACTCA GCGACCTACA AAAACAAGCT 
CACGACCTCC ATAAACAGGT CAATCAGCTG AGCGACCAGG TGTGTGGCGG TCACAGCAAT 
AACAGCGTCA ATGGCAGTTG CCCAGACAGT CAGTGTGGTG GTGCCGGCTG CCATGACAAT 
CAGGGAAACC ATGTATGTGG AGGAGTGGGA TGCAGCGGGA CTGTGGGCAC ATCTGTAGCT 
GCGCTGAATG ACACCAGGAA CGTTACAGAC AGTCTGAACA CTGCCAGTAA GGAGCTGCAG 
AAAGTCTCCA AAAAGCTCCA GGACATAGCT GCTATGACAC AAAAACTGAA GAACCAGACG 
ATAAACACGC TGGAAAAAGC CAACAAGTTG AGCAAGGAGC TTAAAGAAAG CAATAAGAAC 
CTGACAGAGT TCATTGAGAA AATTAAAAAC TTTCTCACTG AGGAGGGAGC CGACCCAGAG 
AGCATAGAGA AAGTGGCTCA GGAGGTCTTG TCGATCACAC TGCCACTGAA CAGGACCGTT 
CTGGACAAAA TGATCGCGCA GATAAAGGAA AGTCTTTCCA ACATCAGCAA TATTGAGGGG 
ATTGTAAATC AAACCACACT GCACACCACT AAAGTCAAGG AGCTTCTTGA TGCAGCTAAA 
GAGGCCAAGA GGCGAGCAGA GGAAGTGAAA GACATGACCA ATATTACCAA GGAGGCGTTA 
AAAGCATCTG GAAAGGCCAT AGAGAAAGCA CAGGCTGCCC TGAAAGAAGC CCACAACAAC 
CTGAACAGCA CAAGAAACGC TACTGCTGAG GTGGAGGGAA AGCTGGTGAG CCTAGACGCC 
AAGCAGATGG ATGTCATGAT GCGTTTAATG AACCTCTCCA AGGAGGTGGA AGCTCTGAAG 
AACAAGACGG AGCAGAACAG ACAGATGGCA ATCGAGGCTA AAGCACTGGC CGACAATGCC 
ACTGACCTAA CATCTTCACT GGAGCAGAGC CTCAACAACA CAGAGAAACA GTATCGTGAG 
CTGCAGATGA AGATCGAGTC TCTTGGTGGG GTGTCTGGAG GTCTGAAGGA CATCAACCAG 
AGGGTCAAAG ATCTCAAAGA GGATGCAGAG AAATGGCTCA AGAATGCCAC CAGTGGGATA 
AGAGAACTGG ACGAACTTGA GGAAAAGTTC AAAAACAATG AGAGGCGGAT GGAGAAGCAG 
CGCTTGGAGT TGGACAAGCT GAAAGACAAC GCAACTAGTG TGCGGGACGA GATCCGGCAT 
CGAGTGCAGA ACTACAACAC CTGTGTGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264755.2
CDS	193..5745
Translation	MAVQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCQNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIHYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRALRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409269.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409269.2

ORF Insert Sequence:

ATGGCTGTGC AAAGAGACAG CCTGTTGTCC ATCATGAATG TTCATCTGAG CCTCCTCATG 
CTGGCTGTCT GTGTATTTGG ACAAGAGCTC CCATCAAGCC CTCATGGCTG CACTGAGGGA 
AGCTGTTACC CTGCAACAGG AAACCTCCTG ATTGGACGTG CTGTCAATCT AACGGCCACC 
TCTACTTGCG GCCTTAATGG ACCAGAGCGA TATTGTATTG TCAGTCATCT GCAGCAACCA 
GAGAAGTGTT TTGAGTGCAA CTCCCAGCAT CCCTACGACC CGAACGTACA CTCGAACAGC 
CACCGCATTG AAAATGTGAT CTACCTCACA GACAGCAACA GAGTCAACAC CTGGTGGCAA 
TCTGTCCATG GCCAGGAGAG TGTCAGCATC AGACTGAACC TGGAGGCTGA ATTCCACTTT 
ACACATCTCA TCATGAAGTT TAAGACTTTC CGACCTGCAG CTCTGGTCAT TGAGCGTTCA 
GCTGACTTTG GCCGAACATG GCGTCCCTAC CGCTACTATG CCTCCAACTG TACCAGAACC 
TTCCCCAGCA TTCCTGCCAA TGGCTTGCAC CATATCAATG ACATCATCTG TGAGGAACGC 
TACTCTGACA TCGAACCCTC CACTAATGGA GAGGTTATTT ATAAAGTGCT GGATCCTGCC 
ATTCCAGTGA AAGACCCCTA CAGCTTAGAT ATTCAAGATC TTTTGCGCAT CACCAACCTG 
CGCATTAACT TCACAAAGCT CAACACTCTG GGAGATGACC TTCTGGACCG ACGCCCTGAA 
GTCCTGCAGA AATATTACTA CGCTATCTAT GAGCTGGTAG TGCGAGGGAG CTGCTTCTGT 
TACGGACATG CTTCAGAGTG CGCTCCTGTG CCGGGGGTCA ACGCCAGGGA GATCGGCATG 
ATCCACGGTC GCTGTGTGTG CAAACACAAC ACAGAAGGCC TGAACTGCGA GCGCTGCCAG 
AACTTCCACC ATGACCTGCC GTGGAGACCA GCGGAGGCCG AGAACCCTCA CACCTGCAGA 
GAGTGTAACT GTAATGGCCA CTCGGACCAC TGCCACTTTG ACATGGCAGT GTATATGGCA 
ACGGGTAATG TTACTGGAGG AGTTTGTGAT GACTGTCTGC ACAACACTAT GGGACGCAAC 
TGTGAGACGT GTAAACCTTT CTACTACCAA GATCCAAACA GAGATGTTAG AGATCCACGG 
GTTTGTATCT CCTGTGACTG TGACCCAGTG GGGTCATTGG AGGGAGGCGT GTGTGACACC 
CACACCAACT TTGAAATGGG AATGATTGCG GGACAGTGTC GCTGCAAAGT CAACGTGAAG 
GGGACGCGCT GTGATGACTG CAAGGAGGGT TATTATGGAC TCAGCCAGAA TGACCCTTTG 
GGTTGCCAAC CTTGCAACTG TGATCCTCGT GGCATCATCG TGATGGGAGC TCCTTGTGAT 
CAGATCAGTG GGGACTGCTC CTGTAAAAGA TATGTCACCG GCCGCTACTG CAACCAGTGT 
CTGCCTGAAT ACTGGGGCCT TAGTAATGAC CTGGCAGGCT GCAGACCTTG TGACTGTGAC 
TTTGGAGGAG CCTTCAACAA CAGGTGCATG ATGGAAACCG GCCAGTGTGA CTGCAGGAGG 
CACCTAATTG GCCGGCAATG CTCTGATGTG CAGCCCGGGT ATTTCTGCGC TCCGCTGGAC 
TTCTACAAGT ATGAGGCCGA AGATGCCACC GGACACTCTC CTGCTGACCC CGTACTGCCG 
GGCCAAGTTC GCCAACAAGC AACTAATGAC TGTGTTCAGC ACCTCAGCAA CCAGCTCAGG 
AGGCACCGGC GGCACCGTAA CATTGCCAAG TCGCAGCAAC ATCAGGCAGC ACTGAGACGT 
ATTCGGCAGC TTCAACAAAC ACCGGATGTG AGGACCGTTC ATAGAGAGCA CACTCTCAGC 
CATATGGTGA CATGGACAGG ACCTGGTTTT TCCCGTGTTA AAGATGGCGC TGGTCTGGTT 
TTCACCATTG ATAACATACC TTATCCCATG GAGTATGACA TCATGATCCG CTATGAACCT 
GAGTCTACAG AGAACTGGGA GGCTGTTGTT AGTATTACAT CTGTAGAATT GCCCTCCAGT 
CTCCGGTGTG GAAACCTCCT ACCTACTGAA CAGCTCTACA CTGTCACTTT GCCACACCAC 
AACAGATACA TCCAGATGCC CAGACCATTT TGTTTTGAGC CCAGCAATCG TTACGTGGTT 
ACAATCAGGT TCCAGCGTCA TGGCGCCACA CACAGACATC TCACTGCCTT CATTCTTATT 
GACTCGCTGG TATTGATCCC TAAATACATG GAGCTTCCTG GGTTTCAAGG TAACACACCA 
GAGTCAGAGC AGCGTCAGGA GGAGATGATC CACTACATGT GTCTTGATTC CTTTATGATC 
ACTCCGATGC CTGCACTGGC TGAGATGTGC TCTAAACTCA TCTGCAGTAT TTCTTCCATT 
ATTCACGACG GTGCCCTGCC CTGTGAGTGT GACCCTCAGG GCTCCCTCAG CGGTGAGTGT 
GACGTAGTGG GAGGTCAGTG CCGTTGTAAA CCCAACGTGA TTGGCAGGCG CTGTGACCAG 
TGTGCCCCGG GGACCTACGG CTTCGGTGTG AGCGGCTGCA CTGCCTGTGA CTGCCACTCC 
GACGGCTCAT TGAGCCACCA GTGTGACCCA GTTACAGGCC AGTGTCAGTG CAGACAAGGA 
GCCACAGGGC GGCAGTGCTC TGACTGTCAG CCGGGCCAGT GGGGCTTCCC CAGCTGCACC 
CCCTGTCAGT GTAACGGCCA TGCAGACCTC TGCGATCCCC GCACGGGAGA ATGCCGAGGC 
TGCAGGGACT TCACAGCAGG ACATCTCTGT GAGCGTTGCA TGGACACGTT CTTTGGAAAC 
CCAGCACTTG GATCAGGGCA GCACTGCCGG CCCTGCCCCT GCCCAAATAA CCCCAACTCC 
GATAACTTCA ATGCGTTATC TTGTCAGGTT GACCACACCT CTGATCAGAT CATGTGTAAC 
TGCAAACAGG GCTACATGGG TCTTCGGTGT GACCAGTGTG CCCCTGGTTA CTATGGTAAC 
CCTGAGCAAC CTGGCGGACA GTGCTTTCCA TGTGAGTGCA ACGGCAACAT AGACACCCAG 
GATCCTGAGT CATGTGATCC CAGGACTGGC GAGTGCATCA AGTGCCTGTA CCACACTGAC 
GGCCCGTCTT GCGCCCAGTG TCAAATCGGT TACTATGGCA ACGCTTTGGC CCACGATTGC 
AGACGCTGTA CCTGTGTGAC TGCTGGTACC CTACAGTCTG CTTGCAGCGA TGGACACTGT 
GACTGTGATC GGCAGGCTGG AGCCTGTCTC TGCAGGGAGC ATGTGGTAGG CCATAACTGT 
GACCAGTGTG CACCTAACTA CTGGAACTTC GGTCAGGATC AGGGCTGTGA GCCGTGTAAT 
TGCAACTCGC AGCACGCTCT GGGAACTCAC TGCAACATGT TCACAGGCCA GTGTCACTGT 
CATTCAGGCT TTGGAGGTAA ACAATGCACC GAGTGTGAGC TGTTCCACTG GGGAGATCCA 
CGGGAGCAGT GCCACGAATG CAACTGTAAC CCAGAGGGCA CAGAGATTGC CCAGTGTGAC 
AGATTAACGG GTGCGTGTGA ATGCCGGGAA GGAGCCGCGG GACTGCAGTG CAACCAGTGC 
GCTCGTGGTT TCACCGGCAT CTTCCCAAAA TGCATCCAGT GTCACCCGTG CTTCCAGCTC 
TGGGATGACG CCCTGTGCCA GATTAGAAGG GACCTGGATC ACATCCAGTC CAGAGTGCAG 
GAGATCCTGA AGGGTGGCAT CACACCGGGA GCTGGGGACG CACGCATCAA AGAACTGGAA 
GGGAAGCTGA AGCAGGTGAA GGATCTGATC ATTACGCAGG ACAGGGACAG GGTCTACCAG 
CTCATTGGCC AGAGCATCGA TGACCTCAGG GCTGAGATTG CCCTCACTGA TGGGCGTCTG 
ATGGCCATGG GCCAAGAGCT GAATACGACA GCAGAAACAG ACAGGGCACT CCGACACACG 
CTGGATAACA TGGAAAAAGA GCTGCGGGAC CTCAACACCA CTGTGACTGA CAAACAGAAG 
CTGCTTGAAC TGTTCGGCTC AGGGTTTGCA GATCAGTTTG AGAAAGTGAA GCAGTACTAT 
CAGGAATCGC TGCAGGCTGA GATGAAATGC AACGCTTCAG TGTTTGGTCC TATGAGTCCT 
GTGGAAGAGT CAAATAAGAC CAGAGCGCGC ACAGAAGACC TGCTGGATGA AATTGAAAAC 
AGCATTAACC GTAATGTGGC TGCTCACAAC AAGTCACTCA GCGACCTACA AAAACAAGCT 
CACGACCTCC ATAAACAGGT CAATCAGCTG AGCGACCAGG TGTGTGGCGG TCACAGCAAT 
AACAGCGTCA ATGGCAGTTG CCCAGACAGT CAGTGTGGTG GTGCCGGCTG CCATGACAAT 
CAGGGAAACC ATGTATGTGG AGGAGTGGGA TGCAGCGGGA CTGTGGGCAC ATCTGTAGCT 
GCGCTGAATG ACACCAGGAA CGTTACAGAC AGTCTGAACA CTGCCAGTAA GGAGCTGCAG 
AAAGTCTCCA AAAAGCTCCA GGACATAGCT GCTATGACAC AAAAACTGAA GAACCAGACG 
ATAAACACGC TGGAAAAAGC CAACAAGTTG AGCAAGGAGC TTAAAGAAAG CAATAAGAAC 
CTGACAGAGT TCATTGAGAA AATTAAAAAC TTTCTCACTG AGGAGGGAGC CGACCCAGAG 
AGCATAGAGA AAGTGGCTCA GGAGGTCTTG TCGATCACAC TGCCACTGAA CAGGACCGTT 
CTGGACAAAA TGATCGCGCA GATAAAGGAA AGTCTTTCCA ACATCAGCAA TATTGAGGGG 
ATTGTAAATC AAACCACACT GCACACCACT AAAGTCAAGG AGCTTCTTGA TGCAGCTAAA 
GAGGCCAAGA GGCGAGCAGA GGAAGTGAAA GACATGACCA ATATTACCAA GGAGGCGTTA 
AAAGCATCTG GAAAGGCCAT AGAGAAAGCA CAGGCTGCCC TGAAAGAAGC CCACAACAAC 
CTGAACAGCA CAAGAAACGC TACTGCTGAG GTGGAGGGAA AGCTGGTGAG CCTAGACGCC 
AAGCAGATGG ATGTCATGAT GCGTTTAATG AACCTCTCCA AGGAGGTGGA AGCTCTGAAG 
AACAAGACGG AGCAGAACAG ACAGATGGCA ATCGAGGCTA AAGCACTGGC CGACAATGCC 
ACTGACCTAA CATCTTCACT GGAGCAGAGC CTCAACAACA CAGAGAAACA GTATCGTGAG 
CTGCAGATGA AGATCGAGTC TCTTGGTGGG GTGTCTGGAG GTCTGAAGGA CATCAACCAG 
AGGGTCAAAG ATCTCAAAGA GGATGCAGAG AAATGGCTCA AGAATGCCAC CAGTGGGATA 
AGAGAACTGG ACGAACTTGA GGAAAAGTTC AAAAACAATG AGAGGCGGAT GGAGAAGCAG 
CGCTTGGAGT TGGACAAGCT GAAAGACAAC GCAACTAGTG TGCGGGACGA GATCCGGCAT 
CGAGTGCAGA ACTACAACAC CTGTGTGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101470954 ( XM_014409270.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014264756.2 Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.

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CloneID	OMa368028
Clone ID Related Accession (Same CDS sequence)	XM_014409270.2
Accession Version	XM_014409270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	laminin subunit beta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036799.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014409270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCAGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCACTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG CACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ACGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014264756.2
CDS	69..5615
Translation	MQRDSLLSIMNVHLSLLMLAVCVFGQELPSSPHGCTEGSCYPATGNLLIGRAVNLTATSTCGLNGPERYCIVSHLQQPEKCFECNSQHPYDPNVHSNSHRIENVIYLTDSNRVNTWWQSVHGQESVSIRLNLEAEFHFTHLIMKFKTFRPAALVIERSADFGRTWRPYRYYASNCTRTFPSIPANGLHHINDIICEERYSDIEPSTNGEVIYKVLDPAIPVKDPYSLDIQDLLRITNLRINFTKLNTLGDDLLDRRPEVLQKYYYAIYELVVRGSCFCYGHASECAPVPGVNAREIGMIHGRCVCKHNTEGLNCERCQNFHHDLPWRPAEAENPHTCRECNCNGHSDHCHFDMAVYMATGNVTGGVCDDCLHNTMGRNCETCKPFYYQDPNRDVRDPRVCISCDCDPVGSLEGGVCDTHTNFEMGMIAGQCRCKVNVKGTRCDDCKEGYYGLSQNDPLGCQPCNCDPRGIIVMGAPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSNDLAGCRPCDCDFGGAFNNRCMMETGQCDCRRHLIGRQCSDVQPGYFCAPLDFYKYEAEDATGHSPADPVLPGQVRQQATNDCVQHLSNQLRRHRRHRNIAKSQQHQAALRRIRQLQQTPDVRTVHREHTLSHMVTWTGPGFSRVKDGAGLVFTIDNIPYPMEYDIMIRYEPESTENWEAVVSITSVELPSSLRCGNLLPTEQLYTVTLPHHNRYIQMPRPFCFEPSNRYVVTIRFQRHGATHRHLTAFILIDSLVLIPKYMELPGFQGNTPESEQRQEEMIHYMCLDSFMITPMPALAEMCSKLICSISSIIHDGALPCECDPQGSLSGECDVVGGQCRCKPNVIGRRCDQCAPGTYGFGVSGCTACDCHSDGSLSHQCDPVTGQCQCRQGATGRQCSDCQPGQWGFPSCTPCQCNGHADLCDPRTGECRGCRDFTAGHLCERCMDTFFGNPALGSGQHCRPCPCPNNPNSDNFNALSCQVDHTSDQIMCNCKQGYMGLRCDQCAPGYYGNPEQPGGQCFPCECNGNIDTQDPESCDPRTGECIKCLYHTDGPSCAQCQIGYYGNALAHDCRRCTCVTAGTLQSACSDGHCDCDRQAGACLCREHVVGHNCDQCAPNYWNFGQDQGCEPCNCNSQHALGTHCNMFTGQCHCHSGFGGKQCTECELFHWGDPREQCHECNCNPEGTEIAQCDRLTGACECREGAAGLQCNQCARGFTGIFPKCIQCHPCFQLWDDALCQIRRDLDHIQSRVQEILKGGITPGAGDARIKELEGKLKQVKDLIITQDRDRVYQLIGQSIDDLRAEIALTDGRLMAMGQELNTTAETDRALRHTLDNMEKELRDLNTTVTDKQKLLELFGSGFADQFEKVKQYYQESLQAEMKCNASVFGPMSPVEESNKTRARTEDLLDEIENSINRNVAAHNKSLSDLQKQAHDLHKQVNQLSDQVCGGHSNNSVNGSCPDSQCGGAGCHDNQGNHVCGGVGCSGTVGTSVAALNDTRNVTDSLNTASKELQKVSKKLQDIAAMTQKLKNQTINTLEKANKLSKELKESNKNLTEFIEKIKNFLTEEGADPESIEKVAQEVLSITLPLNRTVLDKMIAQIKESLSNISNIEGIVNQTTLHTTKVKELLDAAKEAKRRAEEVKDMTNITKEALKASGKAIEKAQAALKEAHNNLNSTRNATAEVEGKLVSLDAKQMDVMMRLMNLSKEVEALKNKTEQNRQMAIEAKALADNATDLTSSLEQSLNNTEKQYRELQMKIESLGGVSGGLKDINQRVKDLKEDAEKWLKNATSGIRELDELEEKFKNNERRMEKQRLELDKLKDNATSVRDEIRHRVQNYNTCVR

Target ORF information:

RefSeq Version	XM_014409270.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra laminin subunit beta-2 (LOC101470954), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014409270.2

ORF Insert Sequence:

ATGCAAAGAG ACAGCCTGTT GTCCATCATG AATGTTCATC TGAGCCTCCT CATGCTGGCT 
GTCTGTGTAT TTGGACAAGA GCTCCCATCA AGCCCTCATG GCTGCACTGA GGGAAGCTGT 
TACCCTGCAA CAGGAAACCT CCTGATTGGA CGTGCTGTCA ATCTAACGGC CACCTCTACT 
TGCGGCCTTA ATGGACCAGA GCGATATTGT ATTGTCAGTC ATCTGCAGCA ACCAGAGAAG 
TGTTTTGAGT GCAACTCCCA GCATCCCTAC GACCCGAACG TACACTCGAA CAGCCACCGC 
ATTGAAAATG TGATCTACCT CACAGACAGC AACAGAGTCA ACACCTGGTG GCAATCTGTC 
CATGGCCAGG AGAGTGTCAG CATCAGACTG AACCTGGAGG CTGAATTCCA CTTTACACAT 
CTCATCATGA AGTTTAAGAC TTTCCGACCT GCAGCTCTGG TCATTGAGCG TTCAGCTGAC 
TTTGGCCGAA CATGGCGTCC CTACCGCTAC TATGCCTCCA ACTGTACCAG AACCTTCCCC 
AGCATTCCTG CCAATGGCTT GCACCATATC AATGACATCA TCTGTGAGGA ACGCTACTCT 
GACATCGAAC CCTCCACTAA TGGAGAGGTT ATTTATAAAG TGCTGGATCC TGCCATTCCA 
GTGAAAGACC CCTACAGCTT AGATATTCAA GATCTTTTGC GCATCACCAA CCTGCGCATT 
AACTTCACAA AGCTCAACAC TCTGGGAGAT GACCTTCTGG ACCGACGCCC TGAAGTCCTG 
CAGAAATATT ACTACGCTAT CTATGAGCTG GTAGTGCGAG GGAGCTGCTT CTGTTACGGA 
CATGCTTCAG AGTGCGCTCC TGTGCCGGGG GTCAACGCCA GGGAGATCGG CATGATCCAC 
GGTCGCTGTG TGTGCAAACA CAACACAGAA GGCCTGAACT GCGAGCGCTG CCAGAACTTC 
CACCATGACC TGCCGTGGAG ACCAGCGGAG GCCGAGAACC CTCACACCTG CAGAGAGTGT 
AACTGTAATG GCCACTCGGA CCACTGCCAC TTTGACATGG CAGTGTATAT GGCAACGGGT 
AATGTTACTG GAGGAGTTTG TGATGACTGT CTGCACAACA CTATGGGACG CAACTGTGAG 
ACGTGTAAAC CTTTCTACTA CCAAGATCCA AACAGAGATG TTAGAGATCC ACGGGTTTGT 
ATCTCCTGTG ACTGTGACCC AGTGGGGTCA TTGGAGGGAG GCGTGTGTGA CACCCACACC 
AACTTTGAAA TGGGAATGAT TGCGGGACAG TGTCGCTGCA AAGTCAACGT GAAGGGGACG 
CGCTGTGATG ACTGCAAGGA GGGTTATTAT GGACTCAGCC AGAATGACCC TTTGGGTTGC 
CAACCTTGCA ACTGTGATCC TCGTGGCATC ATCGTGATGG GAGCTCCTTG TGATCAGATC 
AGTGGGGACT GCTCCTGTAA AAGATATGTC ACCGGCCGCT ACTGCAACCA GTGTCTGCCT 
GAATACTGGG GCCTTAGTAA TGACCTGGCA GGCTGCAGAC CTTGTGACTG TGACTTTGGA 
GGAGCCTTCA ACAACAGGTG CATGATGGAA ACCGGCCAGT GTGACTGCAG GAGGCACCTA 
ATTGGCCGGC AATGCTCTGA TGTGCAGCCC GGGTATTTCT GCGCTCCGCT GGACTTCTAC 
AAGTATGAGG CCGAAGATGC CACCGGACAC TCTCCTGCTG ACCCCGTACT GCCGGGCCAA 
GTTCGCCAAC AAGCAACTAA TGACTGTGTT CAGCACCTCA GCAACCAGCT CAGGAGGCAC 
CGGCGGCACC GTAACATTGC CAAGTCGCAG CAACATCAGG CAGCACTGAG ACGTATTCGG 
CAGCTTCAAC AAACACCGGA TGTGAGGACC GTTCATAGAG AGCACACTCT CAGCCATATG 
GTGACATGGA CAGGACCTGG TTTTTCCCGT GTTAAAGATG GCGCTGGTCT GGTTTTCACC 
ATTGATAACA TACCTTATCC CATGGAGTAT GACATCATGA TCCGCTATGA ACCTGAGTCT 
ACAGAGAACT GGGAGGCTGT TGTTAGTATT ACATCTGTAG AATTGCCCTC CAGTCTCCGG 
TGTGGAAACC TCCTACCTAC TGAACAGCTC TACACTGTCA CTTTGCCACA CCACAACAGA 
TACATCCAGA TGCCCAGACC ATTTTGTTTT GAGCCCAGCA ATCGTTACGT GGTTACAATC 
AGGTTCCAGC GTCATGGCGC CACACACAGA CATCTCACTG CCTTCATTCT TATTGACTCG 
CTGGTATTGA TCCCTAAATA CATGGAGCTT CCTGGGTTTC AAGGTAACAC ACCAGAGTCA 
GAGCAGCGTC AGGAGGAGAT GATCCACTAC ATGTGTCTTG ATTCCTTTAT GATCACTCCG 
ATGCCTGCAC TGGCTGAGAT GTGCTCTAAA CTCATCTGCA GTATTTCTTC CATTATTCAC 
GACGGTGCCC TGCCCTGTGA GTGTGACCCT CAGGGCTCCC TCAGCGGTGA GTGTGACGTA 
GTGGGAGGTC AGTGCCGTTG TAAACCCAAC GTGATTGGCA GGCGCTGTGA CCAGTGTGCC 
CCGGGGACCT ACGGCTTCGG TGTGAGCGGC TGCACTGCCT GTGACTGCCA CTCCGACGGC 
TCATTGAGCC ACCAGTGTGA CCCAGTTACA GGCCAGTGTC AGTGCAGACA AGGAGCCACA 
GGGCGGCAGT GCTCTGACTG TCAGCCGGGC CAGTGGGGCT TCCCCAGCTG CACCCCCTGT 
CAGTGTAACG GCCATGCAGA CCTCTGCGAT CCCCGCACGG GAGAATGCCG AGGCTGCAGG 
GACTTCACAG CAGGACATCT CTGTGAGCGT TGCATGGACA CGTTCTTTGG AAACCCAGCA 
CTTGGATCAG GGCAGCACTG CCGGCCCTGC CCCTGCCCAA ATAACCCCAA CTCCGATAAC 
TTCAATGCGT TATCTTGTCA GGTTGACCAC ACCTCTGATC AGATCATGTG TAACTGCAAA 
CAGGGCTACA TGGGTCTTCG GTGTGACCAG TGTGCCCCTG GTTACTATGG TAACCCTGAG 
CAACCTGGCG GACAGTGCTT TCCATGTGAG TGCAACGGCA ACATAGACAC CCAGGATCCT 
GAGTCATGTG ATCCCAGGAC TGGCGAGTGC ATCAAGTGCC TGTACCACAC TGACGGCCCG 
TCTTGCGCCC AGTGTCAAAT CGGTTACTAT GGCAACGCTT TGGCCCACGA TTGCAGACGC 
TGTACCTGTG TGACTGCTGG TACCCTACAG TCTGCTTGCA GCGATGGACA CTGTGACTGT 
GATCGGCAGG CTGGAGCCTG TCTCTGCAGG GAGCATGTGG TAGGCCATAA CTGTGACCAG 
TGTGCACCTA ACTACTGGAA CTTCGGTCAG GATCAGGGCT GTGAGCCGTG TAATTGCAAC 
TCGCAGCACG CTCTGGGAAC TCACTGCAAC ATGTTCACAG GCCAGTGTCA CTGTCATTCA 
GGCTTTGGAG GTAAACAATG CACCGAGTGT GAGCTGTTCC ACTGGGGAGA TCCACGGGAG 
CAGTGCCACG AATGCAACTG TAACCCAGAG GGCACAGAGA TTGCCCAGTG TGACAGATTA 
ACGGGTGCGT GTGAATGCCG GGAAGGAGCC GCGGGACTGC AGTGCAACCA GTGCGCTCGT 
GGTTTCACCG GCATCTTCCC AAAATGCATC CAGTGTCACC CGTGCTTCCA GCTCTGGGAT 
GACGCCCTGT GCCAGATTAG AAGGGACCTG GATCACATCC AGTCCAGAGT GCAGGAGATC 
CTGAAGGGTG GCATCACACC GGGAGCTGGG GACGCACGCA TCAAAGAACT GGAAGGGAAG 
CTGAAGCAGG TGAAGGATCT GATCATTACG CAGGACAGGG ACAGGGTCTA CCAGCTCATT 
GGCCAGAGCA TCGATGACCT CAGGGCTGAG ATTGCCCTCA CTGATGGGCG TCTGATGGCC 
ATGGGCCAAG AGCTGAATAC GACAGCAGAA ACAGACAGGG CACTCCGACA CACGCTGGAT 
AACATGGAAA AAGAGCTGCG GGACCTCAAC ACCACTGTGA CTGACAAACA GAAGCTGCTT 
GAACTGTTCG GCTCAGGGTT TGCAGATCAG TTTGAGAAAG TGAAGCAGTA CTATCAGGAA 
TCGCTGCAGG CTGAGATGAA ATGCAACGCT TCAGTGTTTG GTCCTATGAG TCCTGTGGAA 
GAGTCAAATA AGACCAGAGC GCGCACAGAA GACCTGCTGG ATGAAATTGA AAACAGCATT 
AACCGTAATG TGGCTGCTCA CAACAAGTCA CTCAGCGACC TACAAAAACA AGCTCACGAC 
CTCCATAAAC AGGTCAATCA GCTGAGCGAC CAGGTGTGTG GCGGTCACAG CAATAACAGC 
GTCAATGGCA GTTGCCCAGA CAGTCAGTGT GGTGGTGCCG GCTGCCATGA CAATCAGGGA 
AACCATGTAT GTGGAGGAGT GGGATGCAGC GGGACTGTGG GCACATCTGT AGCTGCGCTG 
AATGACACCA GGAACGTTAC AGACAGTCTG AACACTGCCA GTAAGGAGCT GCAGAAAGTC 
TCCAAAAAGC TCCAGGACAT AGCTGCTATG ACACAAAAAC TGAAGAACCA GACGATAAAC 
ACGCTGGAAA AAGCCAACAA GTTGAGCAAG GAGCTTAAAG AAAGCAATAA GAACCTGACA 
GAGTTCATTG AGAAAATTAA AAACTTTCTC ACTGAGGAGG GAGCCGACCC AGAGAGCATA 
GAGAAAGTGG CTCAGGAGGT CTTGTCGATC ACACTGCCAC TGAACAGGAC CGTTCTGGAC 
AAAATGATCG CGCAGATAAA GGAAAGTCTT TCCAACATCA GCAATATTGA GGGGATTGTA 
AATCAAACCA CACTGCACAC CACTAAAGTC AAGGAGCTTC TTGATGCAGC TAAAGAGGCC 
AAGAGGCGAG CAGAGGAAGT GAAAGACATG ACCAATATTA CCAAGGAGGC GTTAAAAGCA 
TCTGGAAAGG CCATAGAGAA AGCACAGGCT GCCCTGAAAG AAGCCCACAA CAACCTGAAC 
AGCACAAGAA ACGCTACTGC TGAGGTGGAG GGAAAGCTGG TGAGCCTAGA CGCCAAGCAG 
ATGGATGTCA TGATGCGTTT AATGAACCTC TCCAAGGAGG TGGAAGCTCT GAAGAACAAG 
ACGGAGCAGA ACAGACAGAT GGCAATCGAG GCTAAAGCAC TGGCCGACAA TGCCACTGAC 
CTAACATCTT CACTGGAGCA GAGCCTCAAC AACACAGAGA AACAGTATCG TGAGCTGCAG 
ATGAAGATCG AGTCTCTTGG TGGGGTGTCT GGAGGTCTGA AGGACATCAA CCAGAGGGTC 
AAAGATCTCA AAGAGGATGC AGAGAAATGG CTCAAGAATG CCACCAGTGG GATAAGAGAA 
CTGGACGAAC TTGAGGAAAA GTTCAAAAAC AATGAGAGGC GGATGGAGAA GCAGCGCTTG 
GAGTTGGACA AGCTGAAAGA CAACGCAACT AGTGTGCGGG ACGAGATCCG GCATCGAGTG 
CAGAACTACA ACACCTGTGT GCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101470954 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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