dysf gene cDNA ORF clone, Maylandia zebra(zebra mbuna)

mRNA	Protein	Name
XM_012921816.1	XP_012777270.1	dysferlin isoform X5
XM_014408467.1	XP_014263953.1	dysferlin isoform X1
XM_014408469.3	XP_014263955.1	dysferlin isoform X5
XM_004561112.2	XP_004561169.1	dysferlin isoform X2
XM_004561112.3	XP_004561169.1	dysferlin isoform X2
XM_004561112.4	XP_004561169.1	dysferlin isoform X2
XM_004561113.2	XP_004561170.1	dysferlin isoform X6
XM_012921816.2	XP_012777270.1	dysferlin isoform X6
XM_012921816.3	XP_012777270.1	dysferlin isoform X6
XM_014408467.2	XP_014263953.1	dysferlin isoform X1
XM_014408468.1	XP_014263954.1	dysferlin isoform X3
XM_014408470.3	XP_014263956.1	dysferlin isoform X8
XM_014408469.1	XP_014263955.1	dysferlin isoform X4
XM_023152711.1	XP_023008479.1	dysferlin isoform X4
XM_014408470.2	XP_014263956.1	dysferlin isoform X8
XM_023152711.2	XP_023008479.1	dysferlin isoform X4
XM_004561113.3	XP_004561170.1	dysferlin isoform X7
XM_014408468.2	XP_014263954.1	dysferlin isoform X3
XM_014408469.2	XP_014263955.1	dysferlin isoform X5
XM_014408470.1	XP_014263956.1	dysferlin isoform X7
XM_014408467.3	XP_014263953.1	dysferlin isoform X1
XM_004561113.4	XP_004561170.1	dysferlin isoform X7
XM_014408468.3	XP_014263954.1	dysferlin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa312509	XM_023152711.1 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36074	XM_014408467.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36075	XM_004561112.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36076	XM_014408468.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36077	XM_014408469.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36078	XM_012921816.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36079	XM_004561113.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36080	XM_014408470.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371605	XM_014408467.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371606	XM_004561112.4 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371608	XM_014408468.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371609	XM_023152711.2 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371610	XM_014408469.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371612	XM_012921816.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371614	XM_004561113.4 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa371616	XM_014408470.3 Latest version!	Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMa36075	XM_004561112.2	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36078	XM_012921816.1	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36074	XM_014408467.1	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36079	XM_004561113.2	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36076	XM_014408468.1	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36077	XM_014408469.1	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa36080	XM_014408470.1	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

dysf ( XM_023152711.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008479.1 Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMa312509
Clone ID Related Accession (Same CDS sequence)	XM_023152711.1
Accession Version	XM_023152711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCCGGA GGAGTCAGAC 
CTTCCCTACC CACCGCCTCA GAGAGAGCCA AATGTCTTCA TGATTCCTCA GGGAATCAAA 
CCTGTTCTGC AGAGAACCGC CATCGAGATT TTAGCATGGG GCGTCCGGAA CCTGAAGAGC 
TTCCAGCTGG CCAGCGTTAC CTCCCCCAGC CTGCAGGTGG AGTGTGGAGG CAGCATGGTC 
CAGAGCTGCG TCATCCGCAG TGTGAAGAAG AAGCCTAACT TTGACATTAA TACGCTTCTC 
CTTGATGTGA GACTTCCCCA AGAGGAGCTC TACATGCCGC CAATTGTCAT CAAAGTCATT 
GACAATCGTC AGTTTGGGAG GAAGCCGGTG GTTGGGCAGT GCACCATCCG CTCGCTGGAG 
GAGTACCGCT ACAACCAAGA AGATGAGCAA GCTGTGGAGT TGGAGGGAGA GGAGGAAGGA 
TGGAGACGTG AGACGCCCCA TTTAATTTCT GGGGAGGTTT TTATTGACAT CGATGATGAG 
CAGCCGCTTG TGTCTGACCA GCTTGCGGAT GGAACCAGTT CTGCTATCAT TAACCTTTCA 
ACCTCTAGTT CCAGCCTTCA TGGAGAAGAG ATTATGGACT GGTGGAGTAA GTTCTACGCA 
TCAACTGGAG AGAAAAACAA GTGTGCGACT TATCTGGAGG AAGGCTTTGA CACTCTCAAG 
GTGTATGACC GGGAGCTGGA GAAGATCGAG GATTTTAAAG GACTTTCTGA TTTCTGCCAA 
ACATTCAAAC TGTACAGAGG AAAGACTCAA GATGAAGGAG AAGATCCCTC AGTGGTGGGA 
GAGTTCAAGG GCATGTTTAA GATCTACCCC CTGCCAGATG ACCCCGGTGC CCCTGTTCCC 
CCAAGGCAGT TCAGAAAGCT GCCTCCCAAT GGCATCGAGG AGTGCTTGGT CCGAGTCTAC 
ATCATCCAGG CTCATGGACT CCAGCCCAAA GACCCCAATG GGAAGTGTGA CCCGTATGTG 
AAGATCACGC TGGGGAAGAA GTCAGTCAAC GACCATGAAA ACTACATCCC CTGTACACTG 
GACCCAGTCT TTGGAAAGAT GTTTGAGCTC ACATGTTCTC TCCCACTGGA AAAGGACCTT 
CGCGTGATGC TGTACGACCA CGACATGCTC ACCAAAGACG AAAAGATCGG CGAGACGGTG 
ATTGACCTGG AGAACCGCTT CCTGTCCAAA TATGGAGCCA TGTGTGGCCT GCCACAGTCA 
TATCATGTAT CTGGAGTAAA CCAGTGGCGA GACCAGCTGA CACCCACGCA GCTGCTATAC 
AGATTCTGTG AGAGAAGAAA TCTGCGGAAG CCAGTCTATG AAGACGAAGC AGTCCGCTTC 
AAAGGAGAAC GATACATCGC TGCCGACCTC GAGGACAAAA ATGTGTCCAA ACGTCATCTT 
GGCCCGATTA AGGAGCGACT GTCCCTTCAC ATCTTGAGGA AACTGGGATT GGTACCCGAA 
CATGTGGAAA GCAGACCACT CTACAGCCCG CTCCAACCTG ACATAGAGCA GGGGCGTCTG 
ATGATGTGGG TTGACCTCTT CCCCAAGTCT CTGGGACCAC CTGGACCGCC GTTCAACATC 
ACGCCACGAA AGGCCAAAAA GTTCTTCCTG CGTTGCATCA TCTGGAACAC CAGTGATGTC 
ATCCTGGATG ATGTCAGCAT CAGCGGGGAG AAGATGAGCG ACATTTATGT CAAAGGCTGG 
CTCGACGGAC ATGAACACAA CAAGCAGAAG ACAGACGTCC ACTACCGCTC TCTGGGAGGA 
GAGGGGAACT TCAACTACAG ATTCCTGTTT CCCTTCAACT ACTTACCAGC AGAGCAACTG 
TGCGTCGTCG ACAAAAAGGA ACATTTCTGG AGTCTGGATA AAGCCGAGAC TAAACTGGCT 
CCTAAACTGA CCATTCAGAT CTGGGACAAC GACAAGTTCT CCTTTGACGA CTACCTAGGT 
CACCTGGTGA TGGACCTGAA CCACATGTTG CGTCCAGCAA AGTCTCCAGA GAAGTGTACC 
CTTCAGGTGC TCGACCAGCC ACCGGAAAAA CTGGTGTCTC TGTTTGAACA GAAGACTGTC 
AAAGGCTGGT GGCCCTGCAC CTGCGAGAAC AATGGAGAGA AGATCATCGC TGGGAAGGTG 
GAGATGTCTC TGGAGATAGT GACAGAGCAG GAGCAAGAGG AGAGACCAGC CGGCCTCGGC 
AGAGATGAAC CCAACATGAA CCCTCACCTG GAGGAGCCTC AGCGTCCAGA GACATCCTTC 
CTGTGGTTCT CCTCCCCTTA CAAGACCCTC AAATACATCC TGTGGAGACG GTTCAAGTGG 
TTCATCATTC TCTTCATTTT CCTCTTCTTC ATTATCCTCT TCCTTGGAGT CTTCCTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGATG GTGGGACCAT TTGGACCAGC CAAGGAAACA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008479.1
CDS	205..6570
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDEPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_023152711.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152711.1

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCCGGA GGAGTCAGAC 
CTTCCCTACC CACCGCCTCA GAGAGAGCCA AATGTCTTCA TGATTCCTCA GGGAATCAAA 
CCTGTTCTGC AGAGAACCGC CATCGAGATT TTAGCATGGG GCGTCCGGAA CCTGAAGAGC 
TTCCAGCTGG CCAGCGTTAC CTCCCCCAGC CTGCAGGTGG AGTGTGGAGG CAGCATGGTC 
CAGAGCTGCG TCATCCGCAG TGTGAAGAAG AAGCCTAACT TTGACATTAA TACGCTTCTC 
CTTGATGTGA GACTTCCCCA AGAGGAGCTC TACATGCCGC CAATTGTCAT CAAAGTCATT 
GACAATCGTC AGTTTGGGAG GAAGCCGGTG GTTGGGCAGT GCACCATCCG CTCGCTGGAG 
GAGTACCGCT ACAACCAAGA AGATGAGCAA GCTGTGGAGT TGGAGGGAGA GGAGGAAGGA 
TGGAGACGTG AGACGCCCCA TTTAATTTCT GGGGAGGTTT TTATTGACAT CGATGATGAG 
CAGCCGCTTG TGTCTGACCA GCTTGCGGAT GGAACCAGTT CTGCTATCAT TAACCTTTCA 
ACCTCTAGTT CCAGCCTTCA TGGAGAAGAG ATTATGGACT GGTGGAGTAA GTTCTACGCA 
TCAACTGGAG AGAAAAACAA GTGTGCGACT TATCTGGAGG AAGGCTTTGA CACTCTCAAG 
GTGTATGACC GGGAGCTGGA GAAGATCGAG GATTTTAAAG GACTTTCTGA TTTCTGCCAA 
ACATTCAAAC TGTACAGAGG AAAGACTCAA GATGAAGGAG AAGATCCCTC AGTGGTGGGA 
GAGTTCAAGG GCATGTTTAA GATCTACCCC CTGCCAGATG ACCCCGGTGC CCCTGTTCCC 
CCAAGGCAGT TCAGAAAGCT GCCTCCCAAT GGCATCGAGG AGTGCTTGGT CCGAGTCTAC 
ATCATCCAGG CTCATGGACT CCAGCCCAAA GACCCCAATG GGAAGTGTGA CCCGTATGTG 
AAGATCACGC TGGGGAAGAA GTCAGTCAAC GACCATGAAA ACTACATCCC CTGTACACTG 
GACCCAGTCT TTGGAAAGAT GTTTGAGCTC ACATGTTCTC TCCCACTGGA AAAGGACCTT 
CGCGTGATGC TGTACGACCA CGACATGCTC ACCAAAGACG AAAAGATCGG CGAGACGGTG 
ATTGACCTGG AGAACCGCTT CCTGTCCAAA TATGGAGCCA TGTGTGGCCT GCCACAGTCA 
TATCATGTAT CTGGAGTAAA CCAGTGGCGA GACCAGCTGA CACCCACGCA GCTGCTATAC 
AGATTCTGTG AGAGAAGAAA TCTGCGGAAG CCAGTCTATG AAGACGAAGC AGTCCGCTTC 
AAAGGAGAAC GATACATCGC TGCCGACCTC GAGGACAAAA ATGTGTCCAA ACGTCATCTT 
GGCCCGATTA AGGAGCGACT GTCCCTTCAC ATCTTGAGGA AACTGGGATT GGTACCCGAA 
CATGTGGAAA GCAGACCACT CTACAGCCCG CTCCAACCTG ACATAGAGCA GGGGCGTCTG 
ATGATGTGGG TTGACCTCTT CCCCAAGTCT CTGGGACCAC CTGGACCGCC GTTCAACATC 
ACGCCACGAA AGGCCAAAAA GTTCTTCCTG CGTTGCATCA TCTGGAACAC CAGTGATGTC 
ATCCTGGATG ATGTCAGCAT CAGCGGGGAG AAGATGAGCG ACATTTATGT CAAAGGCTGG 
CTCGACGGAC ATGAACACAA CAAGCAGAAG ACAGACGTCC ACTACCGCTC TCTGGGAGGA 
GAGGGGAACT TCAACTACAG ATTCCTGTTT CCCTTCAACT ACTTACCAGC AGAGCAACTG 
TGCGTCGTCG ACAAAAAGGA ACATTTCTGG AGTCTGGATA AAGCCGAGAC TAAACTGGCT 
CCTAAACTGA CCATTCAGAT CTGGGACAAC GACAAGTTCT CCTTTGACGA CTACCTAGGT 
CACCTGGTGA TGGACCTGAA CCACATGTTG CGTCCAGCAA AGTCTCCAGA GAAGTGTACC 
CTTCAGGTGC TCGACCAGCC ACCGGAAAAA CTGGTGTCTC TGTTTGAACA GAAGACTGTC 
AAAGGCTGGT GGCCCTGCAC CTGCGAGAAC AATGGAGAGA AGATCATCGC TGGGAAGGTG 
GAGATGTCTC TGGAGATAGT GACAGAGCAG GAGCAAGAGG AGAGACCAGC CGGCCTCGGC 
AGAGATGAAC CCAACATGAA CCCTCACCTG GAGGAGCCTC AGCGTCCAGA GACATCCTTC 
CTGTGGTTCT CCTCCCCTTA CAAGACCCTC AAATACATCC TGTGGAGACG GTTCAAGTGG 
TTCATCATTC TCTTCATTTT CCTCTTCTTC ATTATCCTCT TCCTTGGAGT CTTCCTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGATG GTGGGACCAT TTGGACCAGC CAAGGAAACA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408467.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263953.1 Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

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CloneID	OMa36074
Clone ID Related Accession (Same CDS sequence)	XM_014408467.1 , XM_014408467.2
Accession Version	XM_014408467.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263953.1
CDS	190..6597
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408467.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408467.1

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408467.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263953.1 Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

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CloneID	OMa36074
Clone ID Related Accession (Same CDS sequence)	XM_014408467.1 , XM_014408467.2
Accession Version	XM_014408467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263953.1
CDS	205..6612
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408467.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408467.2

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561112.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561169.1 Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

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CloneID	OMa36075
Clone ID Related Accession (Same CDS sequence)	XM_004561112.2 , XM_004561112.3
Accession Version	XM_004561112.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004561112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTAAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ACGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561169.1
CDS	395..6787
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561112.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561112.2

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTAAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ACGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561112.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561169.1 Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

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CloneID	OMa36075
Clone ID Related Accession (Same CDS sequence)	XM_004561112.2 , XM_004561112.3
Accession Version	XM_004561112.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004561112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTAAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ACGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561169.1
CDS	430..6822
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561112.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561112.3

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTAAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ACGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408468.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263954.1 Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

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CloneID	OMa36076
Clone ID Related Accession (Same CDS sequence)	XM_014408468.1 , XM_014408468.2
Accession Version	XM_014408468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263954.1
CDS	190..6564
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408468.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408468.1

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408468.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263954.1 Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

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CloneID	OMa36076
Clone ID Related Accession (Same CDS sequence)	XM_014408468.1 , XM_014408468.2
Accession Version	XM_014408468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263954.1
CDS	205..6579
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408468.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408468.2

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408469.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263955.1 Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

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CloneID	OMa36077
Clone ID Related Accession (Same CDS sequence)	XM_014408469.1 , XM_014408469.2
Accession Version	XM_014408469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263955.1
CDS	190..6537
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408469.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408469.1

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408469.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263955.1 Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

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CloneID	OMa36077
Clone ID Related Accession (Same CDS sequence)	XM_014408469.1 , XM_014408469.2
Accession Version	XM_014408469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263955.1
CDS	205..6552
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408469.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408469.2

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTAA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGACGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_012921816.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777270.1 Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

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CloneID	OMa36078
Clone ID Related Accession (Same CDS sequence)	XM_012921816.1 , XM_012921816.2
Accession Version	XM_012921816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777270.1
CDS	395..6694
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_012921816.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921816.1

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_012921816.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777270.1 Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

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CloneID	OMa36078
Clone ID Related Accession (Same CDS sequence)	XM_012921816.1 , XM_012921816.2
Accession Version	XM_012921816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012921816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777270.1
CDS	430..6729
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_012921816.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921816.2

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561113.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561170.1 Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

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CloneID	OMa36079
Clone ID Related Accession (Same CDS sequence)	XM_004561113.2 , XM_004561113.3
Accession Version	XM_004561113.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004561113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561170.1
CDS	395..6754
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561113.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561113.2

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561113.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561170.1 Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

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CloneID	OMa36079
Clone ID Related Accession (Same CDS sequence)	XM_004561113.2 , XM_004561113.3
Accession Version	XM_004561113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004561113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561170.1
CDS	430..6789
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561113.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561113.3

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT AAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGACG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408470.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263956.1 Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.

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CloneID	OMa36080
Clone ID Related Accession (Same CDS sequence)	XM_014408470.2 , XM_014408470.1
Accession Version	XM_014408470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_014408470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263956.1
CDS	205..6519
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408470.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408470.2

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408470.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263956.1 Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

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CloneID	OMa36080
Clone ID Related Accession (Same CDS sequence)	XM_014408470.2 , XM_014408470.1
Accession Version	XM_014408470.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	dysferlin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447917.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263956.1
CDS	190..6504
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408470.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408470.1

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTAAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGACGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408467.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263953.1 Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

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CloneID	OMa371605
Clone ID Related Accession (Same CDS sequence)	XM_014408467.3
Accession Version	XM_014408467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263953.1
CDS	205..6612
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408467.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408467.3

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGCTTGCGG ATGGAACCAG TTCTGCTATC ATTAACCTTT CAACCTCTAG TTCCAGCCTT 
CATGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561112.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561169.1 Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

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CloneID	OMa371606
Clone ID Related Accession (Same CDS sequence)	XM_004561112.4
Accession Version	XM_004561112.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004561112.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTGAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ATGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561169.1
CDS	430..6822
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561112.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561112.4

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGATGAC TTGACTCCAC GGAGCGTCCA CTCTGGCATG 
GATGACAGTC TTGGTTTCCT GCCAACATTT GGTCCATGTT TTGTAAACCT ATACGGCAGC 
CCCAGAGAGT TCACCGCCTT CAATGACCCC CATGAGGCTC TTAACTTGGG AAAAGGGGAG 
GGCGTGGCAT ACCGAGGCCG GGTTTTACTG GAACTTACCA CGAAACTGGT GGACAAGCCG 
GAGCAAAAAA CTGAAGACAT ACCCTCAGAT GATCTTTTGG TGGTGGAGAA GTTTCTCAGG 
AAGAGGAAGT TCTCGCTCTT CGTTGCATTT TACTCTGCCA CGCTCCTCCA GGACGTCGAC 
GACGCCATCC AGTTTGAAGT CAGCATCGGC AACTACGGCA ACAAGTTCGA CTACACCTGC 
CTTCCTCTGG CCTCCACTAC TCAGTTTAGC AGGGCAGTGT TTGACGGTTG CCACTATTAC 
TACCTCCCAT GGGGAAATGT TAAGCCGGTG GTTGTTCTCT CTTCTGAGTG GGAGGACATC 
AAGCCAAGGA TCGAGGCTCT CAACATGTTG CTGGCCATCA CAGACAGGCT GGAGGCGAAC 
CTGCAGAGGG TGCAGCTGGC AGTGAAGGCT GGCGGTGATC TGGAGGAGGT GGAGCTCAGA 
GTGGTGGAGT TGTTGGACCA GCTTATCAGT GACTGCAGTG ATGAGCTGCC CTCACTCGAT 
CAGTGGCAGT GTGCAACGCC TCTGGATCGC TCGCTGAGGC ACGTTCGTAC CCTCCACCTG 
CAGCAGATCG CCGCGGCGGC GCTGGCTCTC AAACACGGCC CGGAGACTGA GCTCTCCACG 
ATGCTGGAGC AGGCTGAGGA CTGGGCCAAC AGGCTGAGGA CCATGACTGA AGAGCCCCAG 
AACAGCCTTC CAGACATCGT GATATGGATG TTGCAGGGTG ACCGCAGGGT AGCGTACCAC 
CGCATCCCAG CACACACCGT CATCTTCTCC CAGCAGCACT GTGGGAAACA CTGCGGTCAG 
CTGCAGACTG TCTTTCTTAA GTGTCCACAG GGCAGTGGAG GAGAAGCCAA ACTTCCCGGT 
CAGTTGAGGG TTAAAGTTTG GTTTGGATTG GCTGCTGATG TCAAACACTT CAATGAATAT 
GCTGAAGGAA AACTGTCTGT CTTTGCCGAG GCGTATGAGA ACCAGACACG GCTCGCCCTG 
GTGGGCAGCT GGGGAACTAC AGGTTTAACC TACCCCAAGT ATAGCGACAT CACCGGGAGA 
GTTAAATTGC CCAAAGAGAG TTTCAAACCC TCGCCCGGCT GGTCCTGGGC TGGAGACTGG 
TACATCAGCC CCGAGAGGAC ACTCCTGTTT GATGTGGATG CCGGTCACAT GACCTTCACA 
GAGGAAGTGT TTGAAAACCA GATGAGGCTA CCAGGTGGCC AGTGGATCGG CATGCAGGAA 
GGATACACGG ATGTGAACGG GGAGAAAGCG GTGCCAAAGG ACGAGATTGA GTGTCCACCA 
GGTTGGGTAT GGGAGGAGGT GGAGTGGAGT GAGGATCTCA ACAGGGCTGT GGATGATCAA 
GGCTGGGAGT ACGGCATCAC CATTCCCCCA GACCGGCGTC CCAAGTCGTG GGTGCCGGCA 
GAGAAGATGT ACCACACCAA CCGACGAAGA CGATGGATAC GGATGAGGCG ACGAGACCAG 
CAGAAGATGG ATGCTCTGAG AAAGCAGCGT CCAGACGAGG CGGAGAGGGA GGGCTGGGAG 
TACGCCTCGC TTTTTGGCTG GAGGTTCCAC CTCAAGCCCA GGAAGACGGA CAGCTTCAGG 
AGGCGCAGGT GGAGGCGCCG CATGGAGCCG CTGGAGAAGA CAGGACCGGC AGCCATCTTT 
GCCCTTGAGT GTTCTCTGAG CAGTATAGAG GACAAGAATG AAGACAAGTC AGTTACGACC 
ACGTTTGGAG TCAACAGGCC TACCATTTCC TGTTTCTTTG AGCGTGGCAC TCGATACCAT 
CTGCGGTGCT ATCTGTATCA AGCCAGAGAC CTGCTGCCCA TGGACAAAGA CAGCTTCTCA 
GACCCCTACG CGATCGTATC CTTCCTCCAC CAATCCCAGA AGACGGTGAC GGTGCGAAAC 
ACTCTCAATC CCACCTGGGA CCAGACGCTC ATCTTCTATG AAGTGGAGAT TTTTGGCGAC 
TCCGAGGTCA CGATGGCCAG CCCCCCCAAT ATTATAGTGG AACTCTACGA TCAGGACACA 
TACGGAGCTG ATGAGTTCAT GGGTCGCTGC GTGTGCCAGC CCTCACTGAC TCGGTCGCCA 
CGCCTGGCCT GGTTCCCAAT TCGCAGAGGG GACAAGAACG CTGGCGAGCT GCTGGCTGCC 
TTTGAGCTTA TACGCAGAGA AAAGCCGTCA CTTCACCATA TTCCAGGTCA AGAGGGAGAT 
TTCCTGACTT CCACCCACAT CTTAGATGAG CTGATGTTTC CAGGATTCCT CTCTGAAAAC 
CTGCAGCAAT GGCCGGAGGA GTCAGACCTT CCCTACCCAC CGCCTCAGAG AGAGCCAAAT 
GTCTTCATGA TTCCTCAGGG AATCAAACCT GTTCTGCAGA GAACCGCCAT CGAGATTTTA 
GCATGGGGCG TCCGGAACCT GAAGAGCTTC CAGCTGGCCA GCGTTACCTC CCCCAGCCTG 
CAGGTGGAGT GTGGAGGCAG CATGGTCCAG AGCTGCGTCA TCCGCAGTGT GAAGAAGAAG 
CCTAACTTTG ACATTAATAC GCTTCTCCTT GATGTGAGAC TTCCCCAAGA GGAGCTCTAC 
ATGCCGCCAA TTGTCATCAA AGTCATTGAC AATCGTCAGT TTGGGAGGAA GCCGGTGGTT 
GGGCAGTGCA CCATCCGCTC GCTGGAGGAG TACCGCTACA ACCAAGAAGA TGAGCAAGCT 
GTGGAGTTGG AGGGAGAGGA GGAAGGATGG AGACGTGAGA CGCCCCATTT AATTTCTGGG 
GAGGTTTTTA TTGACATCGA TGATGAGCAG CCGCTTGTGT CTGACCAGCT TGCGGATGGA 
ACCAGTTCTG CTATCATTAA CCTTTCAACC TCTAGTTCCA GCCTTCATGG AGAAGAGATT 
ATGGACTGGT GGAGTAAGTT CTACGCATCA ACTGGAGAGA AAAACAAGTG TGCGACTTAT 
CTGGAGGAAG GCTTTGACAC TCTCAAGGTG TATGACCGGG AGCTGGAGAA GATCGAGGAT 
TTTAAAGGAC TTTCTGATTT CTGCCAAACA TTCAAACTGT ACAGAGGAAA GACTCAAGAT 
GAAGGAGAAG ATCCCTCAGT GGTGGGAGAG TTCAAGGGCA TGTTTAAGAT CTACCCCCTG 
CCAGATGACC CCGGTGCCCC TGTTCCCCCA AGGCAGTTCA GAAAGCTGCC TCCCAATGGC 
ATCGAGGAGT GCTTGGTCCG AGTCTACATC ATCCAGGCTC ATGGACTCCA GCCCAAAGAC 
CCCAATGGGA AGTGTGACCC GTATGTGAAG ATCACGCTGG GGAAGAAGTC AGTCAACGAC 
CATGAAAACT ACATCCCCTG TACACTGGAC CCAGTCTTTG GAAAGATGTT TGAGCTCACA 
TGTTCTCTCC CACTGGAAAA GGACCTTCGC GTGATGCTGT ACGACCACGA CATGCTCACC 
AAAGACGAAA AGATCGGCGA GACGGTGATT GACCTGGAGA ACCGCTTCCT GTCCAAATAT 
GGAGCCATGT GTGGCCTGCC ACAGTCATAT CATGTATCTG GAGTAAACCA GTGGCGAGAC 
CAGCTGACAC CCACGCAGCT GCTATACAGA TTCTGTGAGA GAAGAAATCT GCGGAAGCCA 
GTCTATGAAG ACGAAGCAGT CCGCTTCAAA GGAGAACGAT ACATCGCTGC CGACCTCGAG 
GACAAAAATG TGTCCAAACG TCATCTTGGC CCGATTAAGG AGCGACTGTC CCTTCACATC 
TTGAGGAAAC TGGGATTGGT ACCCGAACAT GTGGAAAGCA GACCACTCTA CAGCCCGCTC 
CAACCTGACA TAGAGCAGGG GCGTCTGATG ATGTGGGTTG ACCTCTTCCC CAAGTCTCTG 
GGACCACCTG GACCGCCGTT CAACATCACG CCACGAAAGG CCAAAAAGTT CTTCCTGCGT 
TGCATCATCT GGAACACCAG TGATGTCATC CTGGATGATG TCAGCATCAG CGGGGAGAAG 
ATGAGCGACA TTTATGTCAA AGGCTGGCTC GACGGACATG AACACAACAA GCAGAAGACA 
GACGTCCACT ACCGCTCTCT GGGAGGAGAG GGGAACTTCA ACTACAGATT CCTGTTTCCC 
TTCAACTACT TACCAGCAGA GCAACTGTGC GTCGTCGACA AAAAGGAACA TTTCTGGAGT 
CTGGATAAAG CCGAGACTAA ACTGGCTCCT AAACTGACCA TTCAGATCTG GGACAACGAC 
AAGTTCTCCT TTGACGACTA CCTAGGTCAC CTGGTGATGG ACCTGAACCA CATGTTGCGT 
CCAGCAAAGT CTCCAGAGAA GTGTACCCTT CAGGTGCTCG ACCAGCCACC GGAAAAACTG 
GTGTCTCTGT TTGAACAGAA GACTGTCAAA GGCTGGTGGC CCTGCACCTG CGAGAACAAT 
GGAGAGAAGA TCATCGCTGG GAAGGTGGAG ATGTCTCTGG AGATAGTGAC AGAGCAGGAG 
CAAGAGGAGA GACCAGCCGG CCTCGGCAGA GATGAACCCA ACATGAACCC TCACCTGGAG 
GAGCCTCAGC GTCCAGAGAC ATCCTTCCTG TGGTTCTCCT CCCCTTACAA GACCCTCAAA 
TACATCCTGT GGAGACGGTT CAAGTGGTTC ATCATTCTCT TCATTTTCCT CTTCTTCATT 
ATCCTCTTCC TTGGAGTCTT CCTCTACTCC TTCCCGAACT ATGCTGCCAT GAAGATGGTG 
GGACCATTTG GACCAGCCAA GGAAACAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408468.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263954.1 Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

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CloneID	OMa371608
Clone ID Related Accession (Same CDS sequence)	XM_014408468.3
Accession Version	XM_014408468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408468.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTGAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGATGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263954.1
CDS	205..6579
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408468.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408468.3

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTGAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGATGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG CTTGCGGATG GAACCAGTTC TGCTATCATT 
AACCTTTCAA CCTCTAGTTC CAGCCTTCAT GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_023152711.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008479.1 Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

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CloneID	OMa371609
Clone ID Related Accession (Same CDS sequence)	XM_023152711.2
Accession Version	XM_023152711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023152711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCCGGA GGAGTCAGAC 
CTTCCCTACC CACCGCCTCA GAGAGAGCCA AATGTCTTCA TGATTCCTCA GGGAATCAAA 
CCTGTTCTGC AGAGAACCGC CATCGAGATT TTAGCATGGG GCGTCCGGAA CCTGAAGAGC 
TTCCAGCTGG CCAGCGTTAC CTCCCCCAGC CTGCAGGTGG AGTGTGGAGG CAGCATGGTC 
CAGAGCTGCG TCATCCGCAG TGTGAAGAAG AAGCCTAACT TTGACATTAA TACGCTTCTC 
CTTGATGTGA GACTTCCCCA AGAGGAGCTC TACATGCCGC CAATTGTCAT CAAAGTCATT 
GACAATCGTC AGTTTGGGAG GAAGCCGGTG GTTGGGCAGT GCACCATCCG CTCGCTGGAG 
GAGTACCGCT ACAACCAAGA AGATGAGCAA GCTGTGGAGT TGGAGGGAGA GGAGGAAGGA 
TGGAGACGTG AGACGCCCCA TTTAATTTCT GGGGAGGTTT TTATTGACAT CGATGATGAG 
CAGCCGCTTG TGTCTGACCA GCTTGCGGAT GGAACCAGTT CTGCTATCAT TAACCTTTCA 
ACCTCTAGTT CCAGCCTTCA TGGAGAAGAG ATTATGGACT GGTGGAGTAA GTTCTACGCA 
TCAACTGGAG AGAAAAACAA GTGTGCGACT TATCTGGAGG AAGGCTTTGA CACTCTCAAG 
GTGTATGACC GGGAGCTGGA GAAGATCGAG GATTTTAAAG GACTTTCTGA TTTCTGCCAA 
ACATTCAAAC TGTACAGAGG AAAGACTCAA GATGAAGGAG AAGATCCCTC AGTGGTGGGA 
GAGTTCAAGG GCATGTTTAA GATCTACCCC CTGCCAGATG ACCCCGGTGC CCCTGTTCCC 
CCAAGGCAGT TCAGAAAGCT GCCTCCCAAT GGCATCGAGG AGTGCTTGGT CCGAGTCTAC 
ATCATCCAGG CTCATGGACT CCAGCCCAAA GACCCCAATG GGAAGTGTGA CCCGTATGTG 
AAGATCACGC TGGGGAAGAA GTCAGTCAAC GACCATGAAA ACTACATCCC CTGTACACTG 
GACCCAGTCT TTGGAAAGAT GTTTGAGCTC ACATGTTCTC TCCCACTGGA AAAGGACCTT 
CGCGTGATGC TGTACGACCA CGACATGCTC ACCAAAGACG AAAAGATCGG CGAGACGGTG 
ATTGACCTGG AGAACCGCTT CCTGTCCAAA TATGGAGCCA TGTGTGGCCT GCCACAGTCA 
TATCATGTAT CTGGAGTAAA CCAGTGGCGA GACCAGCTGA CACCCACGCA GCTGCTATAC 
AGATTCTGTG AGAGAAGAAA TCTGCGGAAG CCAGTCTATG AAGACGAAGC AGTCCGCTTC 
AAAGGAGAAC GATACATCGC TGCCGACCTC GAGGACAAAA ATGTGTCCAA ACGTCATCTT 
GGCCCGATTA AGGAGCGACT GTCCCTTCAC ATCTTGAGGA AACTGGGATT GGTACCCGAA 
CATGTGGAAA GCAGACCACT CTACAGCCCG CTCCAACCTG ACATAGAGCA GGGGCGTCTG 
ATGATGTGGG TTGACCTCTT CCCCAAGTCT CTGGGACCAC CTGGACCGCC GTTCAACATC 
ACGCCACGAA AGGCCAAAAA GTTCTTCCTG CGTTGCATCA TCTGGAACAC CAGTGATGTC 
ATCCTGGATG ATGTCAGCAT CAGCGGGGAG AAGATGAGCG ACATTTATGT CAAAGGCTGG 
CTCGACGGAC ATGAACACAA CAAGCAGAAG ACAGACGTCC ACTACCGCTC TCTGGGAGGA 
GAGGGGAACT TCAACTACAG ATTCCTGTTT CCCTTCAACT ACTTACCAGC AGAGCAACTG 
TGCGTCGTCG ACAAAAAGGA ACATTTCTGG AGTCTGGATA AAGCCGAGAC TAAACTGGCT 
CCTAAACTGA CCATTCAGAT CTGGGACAAC GACAAGTTCT CCTTTGACGA CTACCTAGGT 
CACCTGGTGA TGGACCTGAA CCACATGTTG CGTCCAGCAA AGTCTCCAGA GAAGTGTACC 
CTTCAGGTGC TCGACCAGCC ACCGGAAAAA CTGGTGTCTC TGTTTGAACA GAAGACTGTC 
AAAGGCTGGT GGCCCTGCAC CTGCGAGAAC AATGGAGAGA AGATCATCGC TGGGAAGGTG 
GAGATGTCTC TGGAGATAGT GACAGAGCAG GAGCAAGAGG AGAGACCAGC CGGCCTCGGC 
AGAGATGAAC CCAACATGAA CCCTCACCTG GAGGAGCCTC AGCGTCCAGA GACATCCTTC 
CTGTGGTTCT CCTCCCCTTA CAAGACCCTC AAATACATCC TGTGGAGACG GTTCAAGTGG 
TTCATCATTC TCTTCATTTT CCTCTTCTTC ATTATCCTCT TCCTTGGAGT CTTCCTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGATG GTGGGACCAT TTGGACCAGC CAAGGAAACA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008479.1
CDS	205..6570
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDEPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_023152711.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152711.2

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCCGGA GGAGTCAGAC 
CTTCCCTACC CACCGCCTCA GAGAGAGCCA AATGTCTTCA TGATTCCTCA GGGAATCAAA 
CCTGTTCTGC AGAGAACCGC CATCGAGATT TTAGCATGGG GCGTCCGGAA CCTGAAGAGC 
TTCCAGCTGG CCAGCGTTAC CTCCCCCAGC CTGCAGGTGG AGTGTGGAGG CAGCATGGTC 
CAGAGCTGCG TCATCCGCAG TGTGAAGAAG AAGCCTAACT TTGACATTAA TACGCTTCTC 
CTTGATGTGA GACTTCCCCA AGAGGAGCTC TACATGCCGC CAATTGTCAT CAAAGTCATT 
GACAATCGTC AGTTTGGGAG GAAGCCGGTG GTTGGGCAGT GCACCATCCG CTCGCTGGAG 
GAGTACCGCT ACAACCAAGA AGATGAGCAA GCTGTGGAGT TGGAGGGAGA GGAGGAAGGA 
TGGAGACGTG AGACGCCCCA TTTAATTTCT GGGGAGGTTT TTATTGACAT CGATGATGAG 
CAGCCGCTTG TGTCTGACCA GCTTGCGGAT GGAACCAGTT CTGCTATCAT TAACCTTTCA 
ACCTCTAGTT CCAGCCTTCA TGGAGAAGAG ATTATGGACT GGTGGAGTAA GTTCTACGCA 
TCAACTGGAG AGAAAAACAA GTGTGCGACT TATCTGGAGG AAGGCTTTGA CACTCTCAAG 
GTGTATGACC GGGAGCTGGA GAAGATCGAG GATTTTAAAG GACTTTCTGA TTTCTGCCAA 
ACATTCAAAC TGTACAGAGG AAAGACTCAA GATGAAGGAG AAGATCCCTC AGTGGTGGGA 
GAGTTCAAGG GCATGTTTAA GATCTACCCC CTGCCAGATG ACCCCGGTGC CCCTGTTCCC 
CCAAGGCAGT TCAGAAAGCT GCCTCCCAAT GGCATCGAGG AGTGCTTGGT CCGAGTCTAC 
ATCATCCAGG CTCATGGACT CCAGCCCAAA GACCCCAATG GGAAGTGTGA CCCGTATGTG 
AAGATCACGC TGGGGAAGAA GTCAGTCAAC GACCATGAAA ACTACATCCC CTGTACACTG 
GACCCAGTCT TTGGAAAGAT GTTTGAGCTC ACATGTTCTC TCCCACTGGA AAAGGACCTT 
CGCGTGATGC TGTACGACCA CGACATGCTC ACCAAAGACG AAAAGATCGG CGAGACGGTG 
ATTGACCTGG AGAACCGCTT CCTGTCCAAA TATGGAGCCA TGTGTGGCCT GCCACAGTCA 
TATCATGTAT CTGGAGTAAA CCAGTGGCGA GACCAGCTGA CACCCACGCA GCTGCTATAC 
AGATTCTGTG AGAGAAGAAA TCTGCGGAAG CCAGTCTATG AAGACGAAGC AGTCCGCTTC 
AAAGGAGAAC GATACATCGC TGCCGACCTC GAGGACAAAA ATGTGTCCAA ACGTCATCTT 
GGCCCGATTA AGGAGCGACT GTCCCTTCAC ATCTTGAGGA AACTGGGATT GGTACCCGAA 
CATGTGGAAA GCAGACCACT CTACAGCCCG CTCCAACCTG ACATAGAGCA GGGGCGTCTG 
ATGATGTGGG TTGACCTCTT CCCCAAGTCT CTGGGACCAC CTGGACCGCC GTTCAACATC 
ACGCCACGAA AGGCCAAAAA GTTCTTCCTG CGTTGCATCA TCTGGAACAC CAGTGATGTC 
ATCCTGGATG ATGTCAGCAT CAGCGGGGAG AAGATGAGCG ACATTTATGT CAAAGGCTGG 
CTCGACGGAC ATGAACACAA CAAGCAGAAG ACAGACGTCC ACTACCGCTC TCTGGGAGGA 
GAGGGGAACT TCAACTACAG ATTCCTGTTT CCCTTCAACT ACTTACCAGC AGAGCAACTG 
TGCGTCGTCG ACAAAAAGGA ACATTTCTGG AGTCTGGATA AAGCCGAGAC TAAACTGGCT 
CCTAAACTGA CCATTCAGAT CTGGGACAAC GACAAGTTCT CCTTTGACGA CTACCTAGGT 
CACCTGGTGA TGGACCTGAA CCACATGTTG CGTCCAGCAA AGTCTCCAGA GAAGTGTACC 
CTTCAGGTGC TCGACCAGCC ACCGGAAAAA CTGGTGTCTC TGTTTGAACA GAAGACTGTC 
AAAGGCTGGT GGCCCTGCAC CTGCGAGAAC AATGGAGAGA AGATCATCGC TGGGAAGGTG 
GAGATGTCTC TGGAGATAGT GACAGAGCAG GAGCAAGAGG AGAGACCAGC CGGCCTCGGC 
AGAGATGAAC CCAACATGAA CCCTCACCTG GAGGAGCCTC AGCGTCCAGA GACATCCTTC 
CTGTGGTTCT CCTCCCCTTA CAAGACCCTC AAATACATCC TGTGGAGACG GTTCAAGTGG 
TTCATCATTC TCTTCATTTT CCTCTTCTTC ATTATCCTCT TCCTTGGAGT CTTCCTCTAC 
TCCTTCCCGA ACTATGCTGC CATGAAGATG GTGGGACCAT TTGGACCAGC CAAGGAAACA 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408469.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263955.1 Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

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CloneID	OMa371610
Clone ID Related Accession (Same CDS sequence)	XM_014408469.3
Accession Version	XM_014408469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263955.1
CDS	205..6552
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDDDLTPRSVHSGMDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408469.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408469.3

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG ATGACTTGAC TCCACGGAGC 
GTCCACTCTG GCATGGATGA CAGTCTTGGT TTCCTGCCAA CATTTGGTCC ATGTTTTGTA 
AACCTATACG GCAGCCCCAG AGAGTTCACC GCCTTCAATG ACCCCCATGA GGCTCTTAAC 
TTGGGAAAAG GGGAGGGCGT GGCATACCGA GGCCGGGTTT TACTGGAACT TACCACGAAA 
CTGGTGGACA AGCCGGAGCA AAAAACTGAA GACATACCCT CAGATGATCT TTTGGTGGTG 
GAGAAGTTTC TCAGGAAGAG GAAGTTCTCG CTCTTCGTTG CATTTTACTC TGCCACGCTC 
CTCCAGGACG TCGACGACGC CATCCAGTTT GAAGTCAGCA TCGGCAACTA CGGCAACAAG 
TTCGACTACA CCTGCCTTCC TCTGGCCTCC ACTACTCAGT TTAGCAGGGC AGTGTTTGAC 
GGTTGCCACT ATTACTACCT CCCATGGGGA AATGTTAAGC CGGTGGTTGT TCTCTCTTCT 
GAGTGGGAGG ACATCAAGCC AAGGATCGAG GCTCTCAACA TGTTGCTGGC CATCACAGAC 
AGGCTGGAGG CGAACCTGCA GAGGGTGCAG CTGGCAGTGA AGGCTGGCGG TGATCTGGAG 
GAGGTGGAGC TCAGAGTGGT GGAGTTGTTG GACCAGCTTA TCAGTGACTG CAGTGATGAG 
CTGCCCTCAC TCGATCAGTG GCAGTGTGCA ACGCCTCTGG ATCGCTCGCT GAGGCACGTT 
CGTACCCTCC ACCTGCAGCA GATCGCCGCG GCGGCGCTGG CTCTCAAACA CGGCCCGGAG 
ACTGAGCTCT CCACGATGCT GGAGCAGGCT GAGGACTGGG CCAACAGGCT GAGGACCATG 
ACTGAAGAGC CCCAGAACAG CCTTCCAGAC ATCGTGATAT GGATGTTGCA GGGTGACCGC 
AGGGTAGCGT ACCACCGCAT CCCAGCACAC ACCGTCATCT TCTCCCAGCA GCACTGTGGG 
AAACACTGCG GTCAGCTGCA GACTGTCTTT CTTAAGTGTC CACAGGGCAG TGGAGGAGAA 
GCCAAACTTC CCGGTCAGTT GAGGGTTAAA GTTTGGTTTG GATTGGCTGC TGATGTCAAA 
CACTTCAATG AATATGCTGA AGGAAAACTG TCTGTCTTTG CCGAGGCGTA TGAGAACCAG 
ACACGGCTCG CCCTGGTGGG CAGCTGGGGA ACTACAGGTT TAACCTACCC CAAGTATAGC 
GACATCACCG GGAGAGTTAA ATTGCCCAAA GAGAGTTTCA AACCCTCGCC CGGCTGGTCC 
TGGGCTGGAG ACTGGTACAT CAGCCCCGAG AGGACACTCC TGTTTGATGT GGATGCCGGT 
CACATGACCT TCACAGAGGA AGTGTTTGAA AACCAGATGA GGCTACCAGG TGGCCAGTGG 
ATCGGCATGC AGGAAGGATA CACGGATGTG AACGGGGAGA AAGCGGTGCC AAAGGACGAG 
ATTGAGTGTC CACCAGGTTG GGTATGGGAG GAGGTGGAGT GGAGTGAGGA TCTCAACAGG 
GCTGTGGATG ATCAAGGCTG GGAGTACGGC ATCACCATTC CCCCAGACCG GCGTCCCAAG 
TCGTGGGTGC CGGCAGAGAA GATGTACCAC ACCAACCGAC GAAGACGATG GATACGGATG 
AGGCGACGAG ACCAGCAGAA GATGGATGCT CTGAGAAAGC AGCGTCCAGA CGAGGCGGAG 
AGGGAGGGCT GGGAGTACGC CTCGCTTTTT GGCTGGAGGT TCCACCTCAA GCCCAGGAAG 
ACGGACAGCT TCAGGAGGCG CAGGTGGAGG CGCCGCATGG AGCCGCTGGA GAAGACAGGA 
CCGGCAGCCA TCTTTGCCCT TGAGTGTTCT CTGAGCAGTA TAGAGGACAA GAATGAAGAC 
AAGTCAGTTA CGACCACGTT TGGAGTCAAC AGGCCTACCA TTTCCTGTTT CTTTGAGCGT 
GGCACTCGAT ACCATCTGCG GTGCTATCTG TATCAAGCCA GAGACCTGCT GCCCATGGAC 
AAAGACAGCT TCTCAGACCC CTACGCGATC GTATCCTTCC TCCACCAATC CCAGAAGACG 
GTGACGGTGC GAAACACTCT CAATCCCACC TGGGACCAGA CGCTCATCTT CTATGAAGTG 
GAGATTTTTG GCGACTCCGA GGTCACGATG GCCAGCCCCC CCAATATTAT AGTGGAACTC 
TACGATCAGG ACACATACGG AGCTGATGAG TTCATGGGTC GCTGCGTGTG CCAGCCCTCA 
CTGACTCGGT CGCCACGCCT GGCCTGGTTC CCAATTCGCA GAGGGGACAA GAACGCTGGC 
GAGCTGCTGG CTGCCTTTGA GCTTATACGC AGAGAAAAGC CGTCACTTCA CCATATTCCA 
GGTCAAGAGG GAGATTTCCT GACTTCCACC CACATCTTAG ATGAGCTGAT GTTTCCAGGA 
TTCCTCTCTG AAAACCTGCA GCAATGGCCG GAGGAGTCAG ACCTTCCCTA CCCACCGCCT 
CAGAGAGAGC CAAATGTCTT CATGATTCCT CAGGGAATCA AACCTGTTCT GCAGAGAACC 
GCCATCGAGA TTTTAGCATG GGGCGTCCGG AACCTGAAGA GCTTCCAGCT GGCCAGCGTT 
ACCTCCCCCA GCCTGCAGGT GGAGTGTGGA GGCAGCATGG TCCAGAGCTG CGTCATCCGC 
AGTGTGAAGA AGAAGCCTAA CTTTGACATT AATACGCTTC TCCTTGATGT GAGACTTCCC 
CAAGAGGAGC TCTACATGCC GCCAATTGTC ATCAAAGTCA TTGACAATCG TCAGTTTGGG 
AGGAAGCCGG TGGTTGGGCA GTGCACCATC CGCTCGCTGG AGGAGTACCG CTACAACCAA 
GAAGATGAGC AAGCTGTGGA GTTGGAGGGA GAGGAGGAAG GATGGAGACG TGAGACGCCC 
CATTTAATTT CTGGGGAGGT TTTTATTGAC ATCGATGATG AGCAGCCGCT TGTGTCTGAC 
CAGGGAGAAG AGATTATGGA CTGGTGGAGT AAGTTCTACG CATCAACTGG AGAGAAAAAC 
AAGTGTGCGA CTTATCTGGA GGAAGGCTTT GACACTCTCA AGGTGTATGA CCGGGAGCTG 
GAGAAGATCG AGGATTTTAA AGGACTTTCT GATTTCTGCC AAACATTCAA ACTGTACAGA 
GGAAAGACTC AAGATGAAGG AGAAGATCCC TCAGTGGTGG GAGAGTTCAA GGGCATGTTT 
AAGATCTACC CCCTGCCAGA TGACCCCGGT GCCCCTGTTC CCCCAAGGCA GTTCAGAAAG 
CTGCCTCCCA ATGGCATCGA GGAGTGCTTG GTCCGAGTCT ACATCATCCA GGCTCATGGA 
CTCCAGCCCA AAGACCCCAA TGGGAAGTGT GACCCGTATG TGAAGATCAC GCTGGGGAAG 
AAGTCAGTCA ACGACCATGA AAACTACATC CCCTGTACAC TGGACCCAGT CTTTGGAAAG 
ATGTTTGAGC TCACATGTTC TCTCCCACTG GAAAAGGACC TTCGCGTGAT GCTGTACGAC 
CACGACATGC TCACCAAAGA CGAAAAGATC GGCGAGACGG TGATTGACCT GGAGAACCGC 
TTCCTGTCCA AATATGGAGC CATGTGTGGC CTGCCACAGT CATATCATGT ATCTGGAGTA 
AACCAGTGGC GAGACCAGCT GACACCCACG CAGCTGCTAT ACAGATTCTG TGAGAGAAGA 
AATCTGCGGA AGCCAGTCTA TGAAGACGAA GCAGTCCGCT TCAAAGGAGA ACGATACATC 
GCTGCCGACC TCGAGGACAA AAATGTGTCC AAACGTCATC TTGGCCCGAT TAAGGAGCGA 
CTGTCCCTTC ACATCTTGAG GAAACTGGGA TTGGTACCCG AACATGTGGA AAGCAGACCA 
CTCTACAGCC CGCTCCAACC TGACATAGAG CAGGGGCGTC TGATGATGTG GGTTGACCTC 
TTCCCCAAGT CTCTGGGACC ACCTGGACCG CCGTTCAACA TCACGCCACG AAAGGCCAAA 
AAGTTCTTCC TGCGTTGCAT CATCTGGAAC ACCAGTGATG TCATCCTGGA TGATGTCAGC 
ATCAGCGGGG AGAAGATGAG CGACATTTAT GTCAAAGGCT GGCTCGACGG ACATGAACAC 
AACAAGCAGA AGACAGACGT CCACTACCGC TCTCTGGGAG GAGAGGGGAA CTTCAACTAC 
AGATTCCTGT TTCCCTTCAA CTACTTACCA GCAGAGCAAC TGTGCGTCGT CGACAAAAAG 
GAACATTTCT GGAGTCTGGA TAAAGCCGAG ACTAAACTGG CTCCTAAACT GACCATTCAG 
ATCTGGGACA ACGACAAGTT CTCCTTTGAC GACTACCTAG GTCACCTGGT GATGGACCTG 
AACCACATGT TGCGTCCAGC AAAGTCTCCA GAGAAGTGTA CCCTTCAGGT GCTCGACCAG 
CCACCGGAAA AACTGGTGTC TCTGTTTGAA CAGAAGACTG TCAAAGGCTG GTGGCCCTGC 
ACCTGCGAGA ACAATGGAGA GAAGATCATC GCTGGGAAGG TGGAGATGTC TCTGGAGATA 
GTGACAGAGC AGGAGCAAGA GGAGAGACCA GCCGGCCTCG GCAGAGATGA ACCCAACATG 
AACCCTCACC TGGAGGAGCC TCAGCGTCCA GAGACATCCT TCCTGTGGTT CTCCTCCCCT 
TACAAGACCC TCAAATACAT CCTGTGGAGA CGGTTCAAGT GGTTCATCAT TCTCTTCATT 
TTCCTCTTCT TCATTATCCT CTTCCTTGGA GTCTTCCTCT ACTCCTTCCC GAACTATGCT 
GCCATGAAGA TGGTGGGACC ATTTGGACCA GCCAAGGAAA CAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_012921816.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777270.1 Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

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CloneID	OMa371612
Clone ID Related Accession (Same CDS sequence)	XM_012921816.3
Accession Version	XM_012921816.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012921816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT GAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGATG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777270.1
CDS	430..6729
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_012921816.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012921816.3

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT GAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGATG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_004561113.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004561170.1 Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

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CloneID	OMa371614
Clone ID Related Accession (Same CDS sequence)	XM_004561113.4
Accession Version	XM_004561113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004561113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT GAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGATG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004561170.1
CDS	430..6789
Translation	MLRCIIQRANNLKHSDPQASVIFRGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQLADGTSSAIINLSTSSSSLHGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_004561113.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004561113.4

ORF Insert Sequence:

ATGTTACGCT GTATTATTCA GCGAGCCAAC AACTTGAAAC ACTCTGACCC TCAGGCCAGT 
GTCATTTTCA GAGGGTCCAA GAAGAAGACA AAGGTCATCA AAAACAATCC TAACCCTGTG 
TGGAATGAGG GTTTTGAGTG GGATCTGAAG GGGATTCCTC TGGACTCTGG AGCTGAGATT 
CACTGTGTTG TTAAAGATCA TGAGAAAATG GGGAGAAACA GGTTTCTGGG GGAGTGTCGG 
CTAGGTCTTA GAGATGTGCT CAACTCTCCC AATTTCGCCG CCACCTTCAC CGTCTCCTTG 
CTGGACACTA AGAGGAACAA CACAGGGGCC ACGCTGACCC TGCAAGTTTC CTACCTTCCT 
CCTCCGGGAT CGGCTCCGAT GTTTCAGCCT CCTCCCCAGC TGGACCCTCA GCCTCATAAC 
AATCCCAGTG TGGAGCTGGA CACGGTCACA ATGATCTCTC TGGATACTTT GGGCGAGGAG 
GATACAGAGA GTATGATGAT GCTTGAGCCC ACAGAGGACC AGGGAGCAGA CGATGGACGC 
TCCATGGTGA TAATTGGTCC TCAGGGGCCC CCGGGCCAGG AATCCCCCAC CGCCCAGACG 
CCCAAGCGTT CACCGCCATC CTACCACACC CAAGGAGGGC TGAGGAAGAG GAGGAGAGGA 
GCCAGCAGTC AGCCCAAACA GCTACCCAAC AAACCACAGG ATCTACAGAT TCGTGTGCGG 
GTCATTGAGG GACGACAGTT GCCGGGCATC AACATTAAAC CTGTTGTTAA GATAGCAGTT 
GCCGGGCAGT CCAAGAGGAC CCGAATCAGA AAGGGGAACA ACCCAGTCTT TGATGAGACT 
TTCTTCCTGA ACTTCTTCGA GACACCCTCA GACTTGTTTG ACGAACCCAT TTTCATCACT 
GTATGTGACT CTCGTTCACT TAGAACTGAC GCCGTGATAG GTGAATTCAA GATGGATGTG 
GGCACCATCT ACAATGAGCA CAGGCACTGC TTCTTGAGGA AGTGGCTTCT GCTTTGTGAC 
CCTGACGACC TCTCTGCTGG GGTCAAAGGC TACCTGAAGG TCAGCTTGTT TGTCTTAGCT 
GCTGGAGATG AGCCACCGGC TGATAAACGT GAGTGTGTGG AGGATAAAGA GGATATAGAA 
TCAAACCTGT TGAGGCCTGC AGGTCTGTCT TTGAGAGGAG CCTCGTTCAA CCTGAGGATC 
TTCAGAGCTG AAGACCTGCC ACAGATGGAC GATGCCTTCA TGGATGGGAT GAGGCAGATT 
CTTGGCTTTG ATAGCAACAG GAAGAATCTG GTGGATCCTC TGGTGGAGAT TCACTTTGCT 
GGCAAAACGA TTTGCACTAA GACTTTGGAA AAGAATGCCA ATCCACAGTG GAACCAGCGC 
CTGGCCATGC CAGTCAGATT TCCTTCCATG TGTGAGAAGA TGAGGATCAG AATTCTGGAC 
TGGGATAGAG CCAGTCACAA CGACGTTATC GGCACCACCC ACCTCTGTAT GTCCAAGATC 
TCCGCCCCCG GAGGAGAGAT AGATGTGGAT GACAGTCTTG GTTTCCTGCC AACATTTGGT 
CCATGTTTTG TAAACCTATA CGGCAGCCCC AGAGAGTTCA CCGCCTTCAA TGACCCCCAT 
GAGGCTCTTA ACTTGGGAAA AGGGGAGGGC GTGGCATACC GAGGCCGGGT TTTACTGGAA 
CTTACCACGA AACTGGTGGA CAAGCCGGAG CAAAAAACTG AAGACATACC CTCAGATGAT 
CTTTTGGTGG TGGAGAAGTT TCTCAGGAAG AGGAAGTTCT CGCTCTTCGT TGCATTTTAC 
TCTGCCACGC TCCTCCAGGA CGTCGACGAC GCCATCCAGT TTGAAGTCAG CATCGGCAAC 
TACGGCAACA AGTTCGACTA CACCTGCCTT CCTCTGGCCT CCACTACTCA GTTTAGCAGG 
GCAGTGTTTG ACGGTTGCCA CTATTACTAC CTCCCATGGG GAAATGTTAA GCCGGTGGTT 
GTTCTCTCTT CTGAGTGGGA GGACATCAAG CCAAGGATCG AGGCTCTCAA CATGTTGCTG 
GCCATCACAG ACAGGCTGGA GGCGAACCTG CAGAGGGTGC AGCTGGCAGT GAAGGCTGGC 
GGTGATCTGG AGGAGGTGGA GCTCAGAGTG GTGGAGTTGT TGGACCAGCT TATCAGTGAC 
TGCAGTGATG AGCTGCCCTC ACTCGATCAG TGGCAGTGTG CAACGCCTCT GGATCGCTCG 
CTGAGGCACG TTCGTACCCT CCACCTGCAG CAGATCGCCG CGGCGGCGCT GGCTCTCAAA 
CACGGCCCGG AGACTGAGCT CTCCACGATG CTGGAGCAGG CTGAGGACTG GGCCAACAGG 
CTGAGGACCA TGACTGAAGA GCCCCAGAAC AGCCTTCCAG ACATCGTGAT ATGGATGTTG 
CAGGGTGACC GCAGGGTAGC GTACCACCGC ATCCCAGCAC ACACCGTCAT CTTCTCCCAG 
CAGCACTGTG GGAAACACTG CGGTCAGCTG CAGACTGTCT TTCTTAAGTG TCCACAGGGC 
AGTGGAGGAG AAGCCAAACT TCCCGGTCAG TTGAGGGTTA AAGTTTGGTT TGGATTGGCT 
GCTGATGTCA AACACTTCAA TGAATATGCT GAAGGAAAAC TGTCTGTCTT TGCCGAGGCG 
TATGAGAACC AGACACGGCT CGCCCTGGTG GGCAGCTGGG GAACTACAGG TTTAACCTAC 
CCCAAGTATA GCGACATCAC CGGGAGAGTT AAATTGCCCA AAGAGAGTTT CAAACCCTCG 
CCCGGCTGGT CCTGGGCTGG AGACTGGTAC ATCAGCCCCG AGAGGACACT CCTGTTTGAT 
GTGGATGCCG GTCACATGAC CTTCACAGAG GAAGTGTTTG AAAACCAGAT GAGGCTACCA 
GGTGGCCAGT GGATCGGCAT GCAGGAAGGA TACACGGATG TGAACGGGGA GAAAGCGGTG 
CCAAAGGACG AGATTGAGTG TCCACCAGGT TGGGTATGGG AGGAGGTGGA GTGGAGTGAG 
GATCTCAACA GGGCTGTGGA TGATCAAGGC TGGGAGTACG GCATCACCAT TCCCCCAGAC 
CGGCGTCCCA AGTCGTGGGT GCCGGCAGAG AAGATGTACC ACACCAACCG ACGAAGACGA 
TGGATACGGA TGAGGCGACG AGACCAGCAG AAGATGGATG CTCTGAGAAA GCAGCGTCCA 
GACGAGGCGG AGAGGGAGGG CTGGGAGTAC GCCTCGCTTT TTGGCTGGAG GTTCCACCTC 
AAGCCCAGGA AGACGGACAG CTTCAGGAGG CGCAGGTGGA GGCGCCGCAT GGAGCCGCTG 
GAGAAGACAG GACCGGCAGC CATCTTTGCC CTTGAGTGTT CTCTGAGCAG TATAGAGGAC 
AAGAATGAAG ACAAGTCAGT TACGACCACG TTTGGAGTCA ACAGGCCTAC CATTTCCTGT 
TTCTTTGAGC GTGGCACTCG ATACCATCTG CGGTGCTATC TGTATCAAGC CAGAGACCTG 
CTGCCCATGG ACAAAGACAG CTTCTCAGAC CCCTACGCGA TCGTATCCTT CCTCCACCAA 
TCCCAGAAGA CGGTGACGGT GCGAAACACT CTCAATCCCA CCTGGGACCA GACGCTCATC 
TTCTATGAAG TGGAGATTTT TGGCGACTCC GAGGTCACGA TGGCCAGCCC CCCCAATATT 
ATAGTGGAAC TCTACGATCA GGACACATAC GGAGCTGATG AGTTCATGGG TCGCTGCGTG 
TGCCAGCCCT CACTGACTCG GTCGCCACGC CTGGCCTGGT TCCCAATTCG CAGAGGGGAC 
AAGAACGCTG GCGAGCTGCT GGCTGCCTTT GAGCTTATAC GCAGAGAAAA GCCGTCACTT 
CACCATATTC CAGGTCAAGA GGGAGATTTC CTGACTTCCA CCCACATCTT AGATGAGCTG 
ATGTTTCCAG GATTCCTCTC TGAAAACCTG CAGCAATGGC CGGAGGAGTC AGACCTTCCC 
TACCCACCGC CTCAGAGAGA GCCAAATGTC TTCATGATTC CTCAGGGAAT CAAACCTGTT 
CTGCAGAGAA CCGCCATCGA GATTTTAGCA TGGGGCGTCC GGAACCTGAA GAGCTTCCAG 
CTGGCCAGCG TTACCTCCCC CAGCCTGCAG GTGGAGTGTG GAGGCAGCAT GGTCCAGAGC 
TGCGTCATCC GCAGTGTGAA GAAGAAGCCT AACTTTGACA TTAATACGCT TCTCCTTGAT 
GTGAGACTTC CCCAAGAGGA GCTCTACATG CCGCCAATTG TCATCAAAGT CATTGACAAT 
CGTCAGTTTG GGAGGAAGCC GGTGGTTGGG CAGTGCACCA TCCGCTCGCT GGAGGAGTAC 
CGCTACAACC AAGAAGATGA GCAAGCTGTG GAGTTGGAGG GAGAGGAGGA AGGATGGAGA 
CGTGAGACGC CCCATTTAAT TTCTGGGGAG GTTTTTATTG ACATCGATGA TGAGCAGCCG 
CTTGTGTCTG ACCAGCTTGC GGATGGAACC AGTTCTGCTA TCATTAACCT TTCAACCTCT 
AGTTCCAGCC TTCATGGAGA AGAGATTATG GACTGGTGGA GTAAGTTCTA CGCATCAACT 
GGAGAGAAAA ACAAGTGTGC GACTTATCTG GAGGAAGGCT TTGACACTCT CAAGGTGTAT 
GACCGGGAGC TGGAGAAGAT CGAGGATTTT AAAGGACTTT CTGATTTCTG CCAAACATTC 
AAACTGTACA GAGGAAAGAC TCAAGATGAA GGAGAAGATC CCTCAGTGGT GGGAGAGTTC 
AAGGGCATGT TTAAGATCTA CCCCCTGCCA GATGACCCCG GTGCCCCTGT TCCCCCAAGG 
CAGTTCAGAA AGCTGCCTCC CAATGGCATC GAGGAGTGCT TGGTCCGAGT CTACATCATC 
CAGGCTCATG GACTCCAGCC CAAAGACCCC AATGGGAAGT GTGACCCGTA TGTGAAGATC 
ACGCTGGGGA AGAAGTCAGT CAACGACCAT GAAAACTACA TCCCCTGTAC ACTGGACCCA 
GTCTTTGGAA AGATGTTTGA GCTCACATGT TCTCTCCCAC TGGAAAAGGA CCTTCGCGTG 
ATGCTGTACG ACCACGACAT GCTCACCAAA GACGAAAAGA TCGGCGAGAC GGTGATTGAC 
CTGGAGAACC GCTTCCTGTC CAAATATGGA GCCATGTGTG GCCTGCCACA GTCATATCAT 
GTATCTGGAG TAAACCAGTG GCGAGACCAG CTGACACCCA CGCAGCTGCT ATACAGATTC 
TGTGAGAGAA GAAATCTGCG GAAGCCAGTC TATGAAGACG AAGCAGTCCG CTTCAAAGGA 
GAACGATACA TCGCTGCCGA CCTCGAGGAC AAAAATGTGT CCAAACGTCA TCTTGGCCCG 
ATTAAGGAGC GACTGTCCCT TCACATCTTG AGGAAACTGG GATTGGTACC CGAACATGTG 
GAAAGCAGAC CACTCTACAG CCCGCTCCAA CCTGACATAG AGCAGGGGCG TCTGATGATG 
TGGGTTGACC TCTTCCCCAA GTCTCTGGGA CCACCTGGAC CGCCGTTCAA CATCACGCCA 
CGAAAGGCCA AAAAGTTCTT CCTGCGTTGC ATCATCTGGA ACACCAGTGA TGTCATCCTG 
GATGATGTCA GCATCAGCGG GGAGAAGATG AGCGACATTT ATGTCAAAGG CTGGCTCGAC 
GGACATGAAC ACAACAAGCA GAAGACAGAC GTCCACTACC GCTCTCTGGG AGGAGAGGGG 
AACTTCAACT ACAGATTCCT GTTTCCCTTC AACTACTTAC CAGCAGAGCA ACTGTGCGTC 
GTCGACAAAA AGGAACATTT CTGGAGTCTG GATAAAGCCG AGACTAAACT GGCTCCTAAA 
CTGACCATTC AGATCTGGGA CAACGACAAG TTCTCCTTTG ACGACTACCT AGGTCACCTG 
GTGATGGACC TGAACCACAT GTTGCGTCCA GCAAAGTCTC CAGAGAAGTG TACCCTTCAG 
GTGCTCGACC AGCCACCGGA AAAACTGGTG TCTCTGTTTG AACAGAAGAC TGTCAAAGGC 
TGGTGGCCCT GCACCTGCGA GAACAATGGA GAGAAGATCA TCGCTGGGAA GGTGGAGATG 
TCTCTGGAGA TAGTGACAGA GCAGGAGCAA GAGGAGAGAC CAGCCGGCCT CGGCAGAGAT 
GAACCCAACA TGAACCCTCA CCTGGAGGAG CCTCAGCGTC CAGAGACATC CTTCCTGTGG 
TTCTCCTCCC CTTACAAGAC CCTCAAATAC ATCCTGTGGA GACGGTTCAA GTGGTTCATC 
ATTCTCTTCA TTTTCCTCTT CTTCATTATC CTCTTCCTTG GAGTCTTCCT CTACTCCTTC 
CCGAACTATG CTGCCATGAA GATGGTGGGA CCATTTGGAC CAGCCAAGGA AACAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dysf ( XM_014408470.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014263956.1 Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.

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CloneID	OMa371616
Clone ID Related Accession (Same CDS sequence)	XM_014408470.3
Accession Version	XM_014408470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036782.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014408470.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTGAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGATGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014263956.1
CDS	205..6519
Translation	MLRVFILCAERLQTPDDDISDAYCSVTYEGSKKKTKVIKNNPNPVWNEGFEWDLKGIPLDSGAEIHCVVKDHEKMGRNRFLGECRLGLRDVLNSPNFAATFTVSLLDTKRNNTGATLTLQVSYLPPPGSAPMFQPPPQLDPQPHNNPSVELDTVTMISLDTLGEEDTESMMMLEPTEDQGADDGRSMVIIGPQGPPGQESPTAQTPKRSPPSYHTQGGLRKRRRGASSQPKQLPNKPQDLQIRVRVIEGRQLPGINIKPVVKIAVAGQSKRTRIRKGNNPVFDETFFLNFFETPSDLFDEPIFITVCDSRSLRTDAVIGEFKMDVGTIYNEHRHCFLRKWLLLCDPDDLSAGVKGYLKVSLFVLAAGDEPPADKRECVEDKEDIESNLLRPAGLSLRGASFNLRIFRAEDLPQMDDAFMDGMRQILGFDSNRKNLVDPLVEIHFAGKTICTKTLEKNANPQWNQRLAMPVRFPSMCEKMRIRILDWDRASHNDVIGTTHLCMSKISAPGGEIDVDDSLGFLPTFGPCFVNLYGSPREFTAFNDPHEALNLGKGEGVAYRGRVLLELTTKLVDKPEQKTEDIPSDDLLVVEKFLRKRKFSLFVAFYSATLLQDVDDAIQFEVSIGNYGNKFDYTCLPLASTTQFSRAVFDGCHYYYLPWGNVKPVVVLSSEWEDIKPRIEALNMLLAITDRLEANLQRVQLAVKAGGDLEEVELRVVELLDQLISDCSDELPSLDQWQCATPLDRSLRHVRTLHLQQIAAAALALKHGPETELSTMLEQAEDWANRLRTMTEEPQNSLPDIVIWMLQGDRRVAYHRIPAHTVIFSQQHCGKHCGQLQTVFLKCPQGSGGEAKLPGQLRVKVWFGLAADVKHFNEYAEGKLSVFAEAYENQTRLALVGSWGTTGLTYPKYSDITGRVKLPKESFKPSPGWSWAGDWYISPERTLLFDVDAGHMTFTEEVFENQMRLPGGQWIGMQEGYTDVNGEKAVPKDEIECPPGWVWEEVEWSEDLNRAVDDQGWEYGITIPPDRRPKSWVPAEKMYHTNRRRRWIRMRRRDQQKMDALRKQRPDEAEREGWEYASLFGWRFHLKPRKTDSFRRRRWRRRMEPLEKTGPAAIFALECSLSSIEDKNEDKSVTTTFGVNRPTISCFFERGTRYHLRCYLYQARDLLPMDKDSFSDPYAIVSFLHQSQKTVTVRNTLNPTWDQTLIFYEVEIFGDSEVTMASPPNIIVELYDQDTYGADEFMGRCVCQPSLTRSPRLAWFPIRRGDKNAGELLAAFELIRREKPSLHHIPGQEGDFLTSTHILDELMFPGFLSENLQQWPEESDLPYPPPQREPNVFMIPQGIKPVLQRTAIEILAWGVRNLKSFQLASVTSPSLQVECGGSMVQSCVIRSVKKKPNFDINTLLLDVRLPQEELYMPPIVIKVIDNRQFGRKPVVGQCTIRSLEEYRYNQEDEQAVELEGEEEGWRRETPHLISGEVFIDIDDEQPLVSDQGEEIMDWWSKFYASTGEKNKCATYLEEGFDTLKVYDRELEKIEDFKGLSDFCQTFKLYRGKTQDEGEDPSVVGEFKGMFKIYPLPDDPGAPVPPRQFRKLPPNGIEECLVRVYIIQAHGLQPKDPNGKCDPYVKITLGKKSVNDHENYIPCTLDPVFGKMFELTCSLPLEKDLRVMLYDHDMLTKDEKIGETVIDLENRFLSKYGAMCGLPQSYHVSGVNQWRDQLTPTQLLYRFCERRNLRKPVYEDEAVRFKGERYIAADLEDKNVSKRHLGPIKERLSLHILRKLGLVPEHVESRPLYSPLQPDIEQGRLMMWVDLFPKSLGPPGPPFNITPRKAKKFFLRCIIWNTSDVILDDVSISGEKMSDIYVKGWLDGHEHNKQKTDVHYRSLGGEGNFNYRFLFPFNYLPAEQLCVVDKKEHFWSLDKAETKLAPKLTIQIWDNDKFSFDDYLGHLVMDLNHMLRPAKSPEKCTLQVLDQPPEKLVSLFEQKTVKGWWPCTCENNGEKIIAGKVEMSLEIVTEQEQEERPAGLGRDEPNMNPHLEEPQRPETSFLWFSSPYKTLKYILWRRFKWFIILFIFLFFIILFLGVFLYSFPNYAAMKMVGPFGPAKETT

Target ORF information:

RefSeq Version	XM_014408470.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra dysferlin (dysf), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014408470.3

ORF Insert Sequence:

ATGCTACGCG TGTTCATTCT GTGTGCAGAG CGGTTGCAAA CGCCGGACGA TGACATCAGT 
GATGCCTACT GCAGCGTCAC CTATGAAGGG TCCAAGAAGA AGACAAAGGT CATCAAAAAC 
AATCCTAACC CTGTGTGGAA TGAGGGTTTT GAGTGGGATC TGAAGGGGAT TCCTCTGGAC 
TCTGGAGCTG AGATTCACTG TGTTGTTAAA GATCATGAGA AAATGGGGAG AAACAGGTTT 
CTGGGGGAGT GTCGGCTAGG TCTTAGAGAT GTGCTCAACT CTCCCAATTT CGCCGCCACC 
TTCACCGTCT CCTTGCTGGA CACTAAGAGG AACAACACAG GGGCCACGCT GACCCTGCAA 
GTTTCCTACC TTCCTCCTCC GGGATCGGCT CCGATGTTTC AGCCTCCTCC CCAGCTGGAC 
CCTCAGCCTC ATAACAATCC CAGTGTGGAG CTGGACACGG TCACAATGAT CTCTCTGGAT 
ACTTTGGGCG AGGAGGATAC AGAGAGTATG ATGATGCTTG AGCCCACAGA GGACCAGGGA 
GCAGACGATG GACGCTCCAT GGTGATAATT GGTCCTCAGG GGCCCCCGGG CCAGGAATCC 
CCCACCGCCC AGACGCCCAA GCGTTCACCG CCATCCTACC ACACCCAAGG AGGGCTGAGG 
AAGAGGAGGA GAGGAGCCAG CAGTCAGCCC AAACAGCTAC CCAACAAACC ACAGGATCTA 
CAGATTCGTG TGCGGGTCAT TGAGGGACGA CAGTTGCCGG GCATCAACAT TAAACCTGTT 
GTTAAGATAG CAGTTGCCGG GCAGTCCAAG AGGACCCGAA TCAGAAAGGG GAACAACCCA 
GTCTTTGATG AGACTTTCTT CCTGAACTTC TTCGAGACAC CCTCAGACTT GTTTGACGAA 
CCCATTTTCA TCACTGTATG TGACTCTCGT TCACTTAGAA CTGACGCCGT GATAGGTGAA 
TTCAAGATGG ATGTGGGCAC CATCTACAAT GAGCACAGGC ACTGCTTCTT GAGGAAGTGG 
CTTCTGCTTT GTGACCCTGA CGACCTCTCT GCTGGGGTCA AAGGCTACCT GAAGGTCAGC 
TTGTTTGTCT TAGCTGCTGG AGATGAGCCA CCGGCTGATA AACGTGAGTG TGTGGAGGAT 
AAAGAGGATA TAGAATCAAA CCTGTTGAGG CCTGCAGGTC TGTCTTTGAG AGGAGCCTCG 
TTCAACCTGA GGATCTTCAG AGCTGAAGAC CTGCCACAGA TGGACGATGC CTTCATGGAT 
GGGATGAGGC AGATTCTTGG CTTTGATAGC AACAGGAAGA ATCTGGTGGA TCCTCTGGTG 
GAGATTCACT TTGCTGGCAA AACGATTTGC ACTAAGACTT TGGAAAAGAA TGCCAATCCA 
CAGTGGAACC AGCGCCTGGC CATGCCAGTC AGATTTCCTT CCATGTGTGA GAAGATGAGG 
ATCAGAATTC TGGACTGGGA TAGAGCCAGT CACAACGACG TTATCGGCAC CACCCACCTC 
TGTATGTCCA AGATCTCCGC CCCCGGAGGA GAGATAGATG TGGATGACAG TCTTGGTTTC 
CTGCCAACAT TTGGTCCATG TTTTGTAAAC CTATACGGCA GCCCCAGAGA GTTCACCGCC 
TTCAATGACC CCCATGAGGC TCTTAACTTG GGAAAAGGGG AGGGCGTGGC ATACCGAGGC 
CGGGTTTTAC TGGAACTTAC CACGAAACTG GTGGACAAGC CGGAGCAAAA AACTGAAGAC 
ATACCCTCAG ATGATCTTTT GGTGGTGGAG AAGTTTCTCA GGAAGAGGAA GTTCTCGCTC 
TTCGTTGCAT TTTACTCTGC CACGCTCCTC CAGGACGTCG ACGACGCCAT CCAGTTTGAA 
GTCAGCATCG GCAACTACGG CAACAAGTTC GACTACACCT GCCTTCCTCT GGCCTCCACT 
ACTCAGTTTA GCAGGGCAGT GTTTGACGGT TGCCACTATT ACTACCTCCC ATGGGGAAAT 
GTTAAGCCGG TGGTTGTTCT CTCTTCTGAG TGGGAGGACA TCAAGCCAAG GATCGAGGCT 
CTCAACATGT TGCTGGCCAT CACAGACAGG CTGGAGGCGA ACCTGCAGAG GGTGCAGCTG 
GCAGTGAAGG CTGGCGGTGA TCTGGAGGAG GTGGAGCTCA GAGTGGTGGA GTTGTTGGAC 
CAGCTTATCA GTGACTGCAG TGATGAGCTG CCCTCACTCG ATCAGTGGCA GTGTGCAACG 
CCTCTGGATC GCTCGCTGAG GCACGTTCGT ACCCTCCACC TGCAGCAGAT CGCCGCGGCG 
GCGCTGGCTC TCAAACACGG CCCGGAGACT GAGCTCTCCA CGATGCTGGA GCAGGCTGAG 
GACTGGGCCA ACAGGCTGAG GACCATGACT GAAGAGCCCC AGAACAGCCT TCCAGACATC 
GTGATATGGA TGTTGCAGGG TGACCGCAGG GTAGCGTACC ACCGCATCCC AGCACACACC 
GTCATCTTCT CCCAGCAGCA CTGTGGGAAA CACTGCGGTC AGCTGCAGAC TGTCTTTCTT 
AAGTGTCCAC AGGGCAGTGG AGGAGAAGCC AAACTTCCCG GTCAGTTGAG GGTTAAAGTT 
TGGTTTGGAT TGGCTGCTGA TGTCAAACAC TTCAATGAAT ATGCTGAAGG AAAACTGTCT 
GTCTTTGCCG AGGCGTATGA GAACCAGACA CGGCTCGCCC TGGTGGGCAG CTGGGGAACT 
ACAGGTTTAA CCTACCCCAA GTATAGCGAC ATCACCGGGA GAGTTAAATT GCCCAAAGAG 
AGTTTCAAAC CCTCGCCCGG CTGGTCCTGG GCTGGAGACT GGTACATCAG CCCCGAGAGG 
ACACTCCTGT TTGATGTGGA TGCCGGTCAC ATGACCTTCA CAGAGGAAGT GTTTGAAAAC 
CAGATGAGGC TACCAGGTGG CCAGTGGATC GGCATGCAGG AAGGATACAC GGATGTGAAC 
GGGGAGAAAG CGGTGCCAAA GGACGAGATT GAGTGTCCAC CAGGTTGGGT ATGGGAGGAG 
GTGGAGTGGA GTGAGGATCT CAACAGGGCT GTGGATGATC AAGGCTGGGA GTACGGCATC 
ACCATTCCCC CAGACCGGCG TCCCAAGTCG TGGGTGCCGG CAGAGAAGAT GTACCACACC 
AACCGACGAA GACGATGGAT ACGGATGAGG CGACGAGACC AGCAGAAGAT GGATGCTCTG 
AGAAAGCAGC GTCCAGACGA GGCGGAGAGG GAGGGCTGGG AGTACGCCTC GCTTTTTGGC 
TGGAGGTTCC ACCTCAAGCC CAGGAAGACG GACAGCTTCA GGAGGCGCAG GTGGAGGCGC 
CGCATGGAGC CGCTGGAGAA GACAGGACCG GCAGCCATCT TTGCCCTTGA GTGTTCTCTG 
AGCAGTATAG AGGACAAGAA TGAAGACAAG TCAGTTACGA CCACGTTTGG AGTCAACAGG 
CCTACCATTT CCTGTTTCTT TGAGCGTGGC ACTCGATACC ATCTGCGGTG CTATCTGTAT 
CAAGCCAGAG ACCTGCTGCC CATGGACAAA GACAGCTTCT CAGACCCCTA CGCGATCGTA 
TCCTTCCTCC ACCAATCCCA GAAGACGGTG ACGGTGCGAA ACACTCTCAA TCCCACCTGG 
GACCAGACGC TCATCTTCTA TGAAGTGGAG ATTTTTGGCG ACTCCGAGGT CACGATGGCC 
AGCCCCCCCA ATATTATAGT GGAACTCTAC GATCAGGACA CATACGGAGC TGATGAGTTC 
ATGGGTCGCT GCGTGTGCCA GCCCTCACTG ACTCGGTCGC CACGCCTGGC CTGGTTCCCA 
ATTCGCAGAG GGGACAAGAA CGCTGGCGAG CTGCTGGCTG CCTTTGAGCT TATACGCAGA 
GAAAAGCCGT CACTTCACCA TATTCCAGGT CAAGAGGGAG ATTTCCTGAC TTCCACCCAC 
ATCTTAGATG AGCTGATGTT TCCAGGATTC CTCTCTGAAA ACCTGCAGCA ATGGCCGGAG 
GAGTCAGACC TTCCCTACCC ACCGCCTCAG AGAGAGCCAA ATGTCTTCAT GATTCCTCAG 
GGAATCAAAC CTGTTCTGCA GAGAACCGCC ATCGAGATTT TAGCATGGGG CGTCCGGAAC 
CTGAAGAGCT TCCAGCTGGC CAGCGTTACC TCCCCCAGCC TGCAGGTGGA GTGTGGAGGC 
AGCATGGTCC AGAGCTGCGT CATCCGCAGT GTGAAGAAGA AGCCTAACTT TGACATTAAT 
ACGCTTCTCC TTGATGTGAG ACTTCCCCAA GAGGAGCTCT ACATGCCGCC AATTGTCATC 
AAAGTCATTG ACAATCGTCA GTTTGGGAGG AAGCCGGTGG TTGGGCAGTG CACCATCCGC 
TCGCTGGAGG AGTACCGCTA CAACCAAGAA GATGAGCAAG CTGTGGAGTT GGAGGGAGAG 
GAGGAAGGAT GGAGACGTGA GACGCCCCAT TTAATTTCTG GGGAGGTTTT TATTGACATC 
GATGATGAGC AGCCGCTTGT GTCTGACCAG GGAGAAGAGA TTATGGACTG GTGGAGTAAG 
TTCTACGCAT CAACTGGAGA GAAAAACAAG TGTGCGACTT ATCTGGAGGA AGGCTTTGAC 
ACTCTCAAGG TGTATGACCG GGAGCTGGAG AAGATCGAGG ATTTTAAAGG ACTTTCTGAT 
TTCTGCCAAA CATTCAAACT GTACAGAGGA AAGACTCAAG ATGAAGGAGA AGATCCCTCA 
GTGGTGGGAG AGTTCAAGGG CATGTTTAAG ATCTACCCCC TGCCAGATGA CCCCGGTGCC 
CCTGTTCCCC CAAGGCAGTT CAGAAAGCTG CCTCCCAATG GCATCGAGGA GTGCTTGGTC 
CGAGTCTACA TCATCCAGGC TCATGGACTC CAGCCCAAAG ACCCCAATGG GAAGTGTGAC 
CCGTATGTGA AGATCACGCT GGGGAAGAAG TCAGTCAACG ACCATGAAAA CTACATCCCC 
TGTACACTGG ACCCAGTCTT TGGAAAGATG TTTGAGCTCA CATGTTCTCT CCCACTGGAA 
AAGGACCTTC GCGTGATGCT GTACGACCAC GACATGCTCA CCAAAGACGA AAAGATCGGC 
GAGACGGTGA TTGACCTGGA GAACCGCTTC CTGTCCAAAT ATGGAGCCAT GTGTGGCCTG 
CCACAGTCAT ATCATGTATC TGGAGTAAAC CAGTGGCGAG ACCAGCTGAC ACCCACGCAG 
CTGCTATACA GATTCTGTGA GAGAAGAAAT CTGCGGAAGC CAGTCTATGA AGACGAAGCA 
GTCCGCTTCA AAGGAGAACG ATACATCGCT GCCGACCTCG AGGACAAAAA TGTGTCCAAA 
CGTCATCTTG GCCCGATTAA GGAGCGACTG TCCCTTCACA TCTTGAGGAA ACTGGGATTG 
GTACCCGAAC ATGTGGAAAG CAGACCACTC TACAGCCCGC TCCAACCTGA CATAGAGCAG 
GGGCGTCTGA TGATGTGGGT TGACCTCTTC CCCAAGTCTC TGGGACCACC TGGACCGCCG 
TTCAACATCA CGCCACGAAA GGCCAAAAAG TTCTTCCTGC GTTGCATCAT CTGGAACACC 
AGTGATGTCA TCCTGGATGA TGTCAGCATC AGCGGGGAGA AGATGAGCGA CATTTATGTC 
AAAGGCTGGC TCGACGGACA TGAACACAAC AAGCAGAAGA CAGACGTCCA CTACCGCTCT 
CTGGGAGGAG AGGGGAACTT CAACTACAGA TTCCTGTTTC CCTTCAACTA CTTACCAGCA 
GAGCAACTGT GCGTCGTCGA CAAAAAGGAA CATTTCTGGA GTCTGGATAA AGCCGAGACT 
AAACTGGCTC CTAAACTGAC CATTCAGATC TGGGACAACG ACAAGTTCTC CTTTGACGAC 
TACCTAGGTC ACCTGGTGAT GGACCTGAAC CACATGTTGC GTCCAGCAAA GTCTCCAGAG 
AAGTGTACCC TTCAGGTGCT CGACCAGCCA CCGGAAAAAC TGGTGTCTCT GTTTGAACAG 
AAGACTGTCA AAGGCTGGTG GCCCTGCACC TGCGAGAACA ATGGAGAGAA GATCATCGCT 
GGGAAGGTGG AGATGTCTCT GGAGATAGTG ACAGAGCAGG AGCAAGAGGA GAGACCAGCC 
GGCCTCGGCA GAGATGAACC CAACATGAAC CCTCACCTGG AGGAGCCTCA GCGTCCAGAG 
ACATCCTTCC TGTGGTTCTC CTCCCCTTAC AAGACCCTCA AATACATCCT GTGGAGACGG 
TTCAAGTGGT TCATCATTCT CTTCATTTTC CTCTTCTTCA TTATCCTCTT CCTTGGAGTC 
TTCCTCTACT CCTTCCCGAA CTATGCTGCC ATGAAGATGG TGGGACCATT TGGACCAGCC 
AAGGAAACAA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	dysf
Entrez Gene ID	101470638
Full Name	dysferlin
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

dysf cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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