unc80 gene cDNA ORF clone, Maylandia zebra(zebra mbuna)

mRNA	Protein	Name
XM_023153469.1	XP_023009237.1	protein unc-80 homolog isoform X11
XM_023153470.1	XP_023009238.1	protein unc-80 homolog isoform X12
XM_012922259.3	XP_012777713.2	protein unc-80 homolog isoform X8
XM_004562575.5	XP_004562632.2	protein unc-80 homolog isoform X2
XM_004562576.4	XP_004562633.2	protein unc-80 homolog isoform X3
XM_004562579.4	XP_004562636.2	protein unc-80 homolog isoform X6
XM_004562580.4	XP_004562637.2	protein unc-80 homolog isoform X9
XM_004562581.3	XP_004562638.1	protein unc-80 homolog isoform X10
XM_004562574.3	XP_004562631.1	protein unc-80 homolog isoform X1
XM_004562574.5	XP_004562631.2	protein unc-80 homolog isoform X1
XM_004562584.4	XP_004562641.2	protein unc-80 homolog isoform X14
XM_012922258.3	XP_012777712.2	protein unc-80 homolog isoform X7
XM_012922262.2	XP_012777716.1	protein unc-80 homolog isoform X13
XM_012922263.3	XP_012777717.2	protein unc-80 homolog isoform X16
XM_012922264.2	XP_012777718.1	protein unc-80 homolog isoform X17
XM_012922264.3	XP_012777718.2	protein unc-80 homolog isoform X17
XM_004562576.3	XP_004562633.1	protein unc-80 homolog isoform X3
XM_004562578.3	XP_004562635.1	protein unc-80 homolog isoform X5
XM_023153469.2	XP_023009237.2	protein unc-80 homolog isoform X11
XM_023153470.2	XP_023009238.2	protein unc-80 homolog isoform X12
XM_004562577.4	XP_004562634.1	protein unc-80 homolog isoform X4
XM_004562580.3	XP_004562637.1	protein unc-80 homolog isoform X9
XM_004562577.3	XP_004562634.1	protein unc-80 homolog isoform X4
XM_004562574.4	XP_004562631.1	protein unc-80 homolog isoform X1
XM_004562575.4	XP_004562632.1	protein unc-80 homolog isoform X2
XM_012922258.2	XP_012777712.1	protein unc-80 homolog isoform X7
XM_012922265.2	XP_012777719.1	protein unc-80 homolog isoform X18
XM_012922265.3	XP_012777719.2	protein unc-80 homolog isoform X18
XM_004562575.3	XP_004562632.1	protein unc-80 homolog isoform X2
XM_004562577.5	XP_004562634.2	protein unc-80 homolog isoform X4
XM_012922259.2	XP_012777713.1	protein unc-80 homolog isoform X8
XM_012922262.3	XP_012777716.1	protein unc-80 homolog isoform X15
XM_004562578.4	XP_004562635.2	protein unc-80 homolog isoform X5
XM_004562579.3	XP_004562636.1	protein unc-80 homolog isoform X6
XM_004562581.4	XP_004562638.2	protein unc-80 homolog isoform X10
XM_004562584.3	XP_004562641.1	protein unc-80 homolog isoform X14
XM_012922261.2	XP_012777715.1	protein unc-80 homolog isoform X11
XM_012922261.3	XP_012777715.1	protein unc-80 homolog isoform X13
XM_012922261.4	XP_012777715.2	protein unc-80 homolog isoform X13
XM_012922262.4	XP_012777716.2	protein unc-80 homolog isoform X15
XM_012922263.2	XP_012777717.1	protein unc-80 homolog isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa09667	XM_004562574.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09668	XM_004562575.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09669	XM_004562576.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09670	XM_004562577.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09671	XM_004562578.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09672	XM_004562579.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09673	XM_012922258.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09674	XM_012922259.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09675	XM_004562580.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09676	XM_004562581.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09677	XM_012922261.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09678	XM_004562584.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09679	XM_012922262.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09680	XM_012922263.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09681	XM_012922264.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09682	XM_012922265.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa313319	XM_023153469.1 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa313320	XM_023153470.1 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370045	XM_004562574.5 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370046	XM_004562575.5 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370047	XM_004562576.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370049	XM_004562577.5 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370051	XM_004562578.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370052	XM_004562579.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370053	XM_012922258.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370054	XM_012922259.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370055	XM_004562580.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370056	XM_004562581.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370057	XM_023153469.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370058	XM_023153470.2 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370059	XM_012922261.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370060	XM_004562584.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370062	XM_012922262.4 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370064	XM_012922263.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370066	XM_012922264.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa370067	XM_012922265.3 Latest version!	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMa09670	XM_004562577.3	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09667	XM_004562574.3	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09668	XM_004562575.3	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09679	XM_012922262.2	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMa09677	XM_012922261.2	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

unc80 ( XM_004562574.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562631.1 Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa09667
Clone ID Related Accession (Same CDS sequence)	XM_004562574.3 , XM_004562574.4
Accession Version	XM_004562574.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	protein unc-80 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562631.1
CDS	398..10888
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562574.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562574.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562574.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562631.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.

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CloneID	OMa09667
Clone ID Related Accession (Same CDS sequence)	XM_004562574.3 , XM_004562574.4
Accession Version	XM_004562574.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004562574.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562631.1
CDS	399..10889
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562574.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562574.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562575.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562632.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.

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CloneID	OMa09668
Clone ID Related Accession (Same CDS sequence)	XM_004562575.4 , XM_004562575.3
Accession Version	XM_004562575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004562575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGCGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTACAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGACGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562632.1
CDS	399..10886
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562575.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562575.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGCGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTACAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGACGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562575.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562632.1 Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X2, mRNA.

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CloneID	OMa09668
Clone ID Related Accession (Same CDS sequence)	XM_004562575.4 , XM_004562575.3
Accession Version	XM_004562575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	protein unc-80 homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGCGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTACAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGACGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562632.1
CDS	398..10885
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562575.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562575.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGCGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTACAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGACGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562576.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562633.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.

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CloneID	OMa09669
Clone ID Related Accession (Same CDS sequence)	XM_004562576.3
Accession Version	XM_004562576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAC GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562633.1
CDS	398..10867
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562576.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562576.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAC GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562577.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562634.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.

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CloneID	OMa09670
Clone ID Related Accession (Same CDS sequence)	XM_004562577.4 , XM_004562577.3
Accession Version	XM_004562577.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004562577.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562634.1
CDS	399..10859
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562577.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562577.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562577.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562634.1 Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X4, mRNA.

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CloneID	OMa09670
Clone ID Related Accession (Same CDS sequence)	XM_004562577.4 , XM_004562577.3
Accession Version	XM_004562577.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	protein unc-80 homolog isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562634.1
CDS	398..10858
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562577.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562577.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562578.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562635.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.

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CloneID	OMa09671
Clone ID Related Accession (Same CDS sequence)	XM_004562578.3
Accession Version	XM_004562578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562635.1
CDS	399..10859
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562578.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562578.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562579.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562636.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.

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CloneID	OMa09672
Clone ID Related Accession (Same CDS sequence)	XM_004562579.3
Accession Version	XM_004562579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG 
AGACAGGTGG AGTGGGAGGC GGCCAGTAGC CTGATCGAGG GGATCTGCCT GACCTTGCAG 
CGACAGCCGA TCATCTCGTT CCTGCCTCAC CTGCGCTCGC TTATCAACGT CTGCGTCAAC 
CTGGTGATGG GTGTGGTCGG CCCCTCCAGT GTGGCGGACG GGCTGCCACT CCTCCACCTC 
AGCCCTTACC TGTCTCCGCC TCTGCCCTTT AGCACCGCCG TGGTCCGATT GGTTGCTCTG 
CAGATTCAGG CCTTGAAGGA AGACTTCCCC CTCAGTCACG TGATCTCACC TTTCACCAAT 
CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC CTGCTCATTC CCTTCGTGTT AACTGTAGGC 
TCCGGGAGCA AAGACAGCCC CCACCTGGAG CAGCCGGAGG TCTTCCTGCT CCTACAGACA 
GTCATCAACA TCCTGCTTCC CCCTCGTATC ATCTCCACAT CCCGCACCAA GAACTTTATG 
CTTGACGCCT CTCCGGCTCA CTGCTCCACG CCGGGTGACC CTGGGAAAGA TCTGCGTAGG 
GAGGGATTAG CAGAGTCCAC GAGCCAGGCT GCTTATTTAG CTCTGAAGGT GGTGCTGGTT 
TGCTTCGAGC GCTCGCTGGG GAACCAGTGG TATCGGCTGA GCCTGCAGGT GAAGGAGATG 
GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC TGGGACTTCA TCGACTTTAT CGTCCGAACC 
CGGATCCCTA TTTTTGTCCT GCTCAGACCC TTTATACAGT GCAAGTTGCT GACCCAGCCG 
GCCGATTCCC AGGAAGAGAT CACAGCGCGC CACCACATCG CTGATCAGCT GGAGCGGCGC 
TTCATCCCAC GACCTCTCTG CAAGAGCTCA CTGTTTGCAG AGTTCAACAA TGAGCTCAAG 
ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA GCATATCAGG GCAAGACATC CATCAGCACG 
GTGGGCACCT CCACGTCAGC CTACCGCCTC AGTTTGGCCA CAATGTCGCG ATCCAACACA 
GGCACTGGAA CAGTTTGGGA GCAGGACAGC CAACCGTCAC GGCAGCCCTC ACAGGACACG 
CTCAGCCGTA CCGACGAGGA CGATGAAGAG ATTGACTCCA TGAGCATTCC CAGCGTGGTG 
AGCGAGCACG AAGCCTTCAT GCCCAGGGTG ATGTCACAGC GCCGTTTCTC CAGCCACGCC 
ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC CCCCGCACCA CCATGTTGCC CAGCCACAGT 
GAACCCAATG TGCTAGATGA GTCCCAGGGT CTTCTGCAGG AGGGTAACCT CTCTAGGGTG 
GCCAGTGTGC AGAGCGAACC AGGTCAGAAC CTCCTGATCC AGCCCCCGTT AGGAAGAAAG 
CGAGGACTCA GACAGATGCG ACGCCCCCTG CTGTCCATTC CAAAAACCGA ACCACGCGGT 
CGATCTGGAG CGAGGCTTTC TACAACGCGC AGGAGCATCC AGCCCAAGAA CAAACCACTG 
GAAACTTTAT TGGACTGTGA AGCGCATGGA GACCAAAAGA GGTCAGTCAC CTTTACAGAG 
AATCAAGGCC AGCAGGCATC CACGGGGGCA AGCAAGCCTC CAACTCCCAG CACTGTGGAC 
CCACCGGGCG AAGGCAGAGG GACGAGTCCT GCCCCATCAA AGTCCCCAAC CCTGGAAGTA 
CCCCCCACCT CTTCAGCCAT GATCTTGGCT GACCTGCACG GACCTACAAA TGCACAGGTC 
CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA GAGCAGTGGG GGCTACGCAG CAGCCTCTCC 
CCTCCCCCAT CCATCTCCCG GCCTTCCACT CCAACACCCC CATCCCGAAC CTGCTCCCCT 
CTCCCCCTCT CCAGAACCTG CTCCCCTCTC CCTTTGTCCC GCACCTCCTC TCCTCTTCCA 
CCGCCACTCC CAGTGCTGAG CACAGCACCC GCTCCGGGTC CACCGCCGCC ACCTCCATTA 
CCACCAACTC ATCTTGCTGC TGCTCCTCCT CCTCCACCAC CCCCGGGGCC TTCTAGGATC 
AGAGAGAGCC CCGGGGACGA GGACACAACC GCCCTGCTGC CGCGCAGCGA CACCCTGCTG 
CACCTCAGCG AGGACGACGG CACCGAGAAC CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT 
CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC CTCCCCCTCT CACCCGTCGA CCTGGACCTG 
GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562636.1
CDS	398..10855
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562579.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562579.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG 
AGACAGGTGG AGTGGGAGGC GGCCAGTAGC CTGATCGAGG GGATCTGCCT GACCTTGCAG 
CGACAGCCGA TCATCTCGTT CCTGCCTCAC CTGCGCTCGC TTATCAACGT CTGCGTCAAC 
CTGGTGATGG GTGTGGTCGG CCCCTCCAGT GTGGCGGACG GGCTGCCACT CCTCCACCTC 
AGCCCTTACC TGTCTCCGCC TCTGCCCTTT AGCACCGCCG TGGTCCGATT GGTTGCTCTG 
CAGATTCAGG CCTTGAAGGA AGACTTCCCC CTCAGTCACG TGATCTCACC TTTCACCAAT 
CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC CTGCTCATTC CCTTCGTGTT AACTGTAGGC 
TCCGGGAGCA AAGACAGCCC CCACCTGGAG CAGCCGGAGG TCTTCCTGCT CCTACAGACA 
GTCATCAACA TCCTGCTTCC CCCTCGTATC ATCTCCACAT CCCGCACCAA GAACTTTATG 
CTTGACGCCT CTCCGGCTCA CTGCTCCACG CCGGGTGACC CTGGGAAAGA TCTGCGTAGG 
GAGGGATTAG CAGAGTCCAC GAGCCAGGCT GCTTATTTAG CTCTGAAGGT GGTGCTGGTT 
TGCTTCGAGC GCTCGCTGGG GAACCAGTGG TATCGGCTGA GCCTGCAGGT GAAGGAGATG 
GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC TGGGACTTCA TCGACTTTAT CGTCCGAACC 
CGGATCCCTA TTTTTGTCCT GCTCAGACCC TTTATACAGT GCAAGTTGCT GACCCAGCCG 
GCCGATTCCC AGGAAGAGAT CACAGCGCGC CACCACATCG CTGATCAGCT GGAGCGGCGC 
TTCATCCCAC GACCTCTCTG CAAGAGCTCA CTGTTTGCAG AGTTCAACAA TGAGCTCAAG 
ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA GCATATCAGG GCAAGACATC CATCAGCACG 
GTGGGCACCT CCACGTCAGC CTACCGCCTC AGTTTGGCCA CAATGTCGCG ATCCAACACA 
GGCACTGGAA CAGTTTGGGA GCAGGACAGC CAACCGTCAC GGCAGCCCTC ACAGGACACG 
CTCAGCCGTA CCGACGAGGA CGATGAAGAG ATTGACTCCA TGAGCATTCC CAGCGTGGTG 
AGCGAGCACG AAGCCTTCAT GCCCAGGGTG ATGTCACAGC GCCGTTTCTC CAGCCACGCC 
ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC CCCCGCACCA CCATGTTGCC CAGCCACAGT 
GAACCCAATG TGCTAGATGA GTCCCAGGGT CTTCTGCAGG AGGGTAACCT CTCTAGGGTG 
GCCAGTGTGC AGAGCGAACC AGGTCAGAAC CTCCTGATCC AGCCCCCGTT AGGAAGAAAG 
CGAGGACTCA GACAGATGCG ACGCCCCCTG CTGTCCATTC CAAAAACCGA ACCACGCGGT 
CGATCTGGAG CGAGGCTTTC TACAACGCGC AGGAGCATCC AGCCCAAGAA CAAACCACTG 
GAAACTTTAT TGGACTGTGA AGCGCATGGA GACCAAAAGA GGTCAGTCAC CTTTACAGAG 
AATCAAGGCC AGCAGGCATC CACGGGGGCA AGCAAGCCTC CAACTCCCAG CACTGTGGAC 
CCACCGGGCG AAGGCAGAGG GACGAGTCCT GCCCCATCAA AGTCCCCAAC CCTGGAAGTA 
CCCCCCACCT CTTCAGCCAT GATCTTGGCT GACCTGCACG GACCTACAAA TGCACAGGTC 
CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA GAGCAGTGGG GGCTACGCAG CAGCCTCTCC 
CCTCCCCCAT CCATCTCCCG GCCTTCCACT CCAACACCCC CATCCCGAAC CTGCTCCCCT 
CTCCCCCTCT CCAGAACCTG CTCCCCTCTC CCTTTGTCCC GCACCTCCTC TCCTCTTCCA 
CCGCCACTCC CAGTGCTGAG CACAGCACCC GCTCCGGGTC CACCGCCGCC ACCTCCATTA 
CCACCAACTC ATCTTGCTGC TGCTCCTCCT CCTCCACCAC CCCCGGGGCC TTCTAGGATC 
AGAGAGAGCC CCGGGGACGA GGACACAACC GCCCTGCTGC CGCGCAGCGA CACCCTGCTG 
CACCTCAGCG AGGACGACGG CACCGAGAAC CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT 
CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC CTCCCCCTCT CACCCGTCGA CCTGGACCTG 
GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922258.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777712.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.

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CloneID	OMa09673
Clone ID Related Accession (Same CDS sequence)	XM_012922258.2
Accession Version	XM_012922258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGAAGAAGTG 
GTGAACCTGG CATCACGACG TCTGTCTGTC AGCCCATCCT GCGCCTCCAG CAACTCCCAC 
AGGAACTACT CTTTCCGCCG GGGCTCTGTA TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA 
GATGAAGAAA ACACAACAGA ACACACACCC ACTCACCACA TGCTACAGCC ACCTCAAGCT 
GTCTTCCCAG CATGCATCTG TGCTGCCGTC CTACCAATAG TGCACCTCAT GGAAGACGGC 
GAAGTTAGAG AGGATGGCGT GGCTGTGAGT GCTGTTGCCC AGCAAATCCT CTGGAACTGC 
CTCATTGAAG ATCCAGCTCT CGTTCTCCGC CACTTCCTGG AAAAGCTGAC AGTTAGCAAC 
AGACAGGACG AGCTGATGTA CATGCTTAGG AAGCTCCTGC TCAACATTGG AGATCTGCCA 
GCCCAGACCT CTCACATCCT TTTTAACTAC CTGGTGGGCC TGATCATGTA TTTTGTGCGG 
ACTCCCTGTG AATGGGGCAT GGACGCCATC TCGGCCACGC TGACCTTCCT CTGGGAGGTG 
GTCGGCTACG TGGAGGGGCT CTTCTTTAAA GACCTGAAGC AGACCATGAA GAAAGAGCAG 
TGTGAGGTCA AACTGCTGGT CACAGCATCG ATGCCAGGAA CCAAAACGCT GGTGGTGCAC 
GGGCAGAATG AATGTGACAT CCCAACCCAG CTACCAGTCC ACGAAGACAC TCAGTTTGAA 
GCCCTGCTAA AGGAGTGTCT GGAATTTTTC AACATTCCCG AGGCCAGATC GGCACACTAC 
TTCCTCATGG ATAAACGATG GAACCTCATC CATTATTCAA AGACATATGT TAGAGACATC 
TACCCGTTCC GAAGGTCAGT CTCTCCTCAG CTCAACCTGG TCCACATGTT GCCTGAGAAA 
GGACAGGAGC TCATCCAGAA ACAGGTGTTT TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC 
CTCTTCCTCA TCTCCCTCAC ACAACACATG CCAGCTGTGC ACAAGCAGTC CCACGTCTCC 
CTGCTGCAGG AGGACCTGCT TCGCCTGCCC TCCTTCCCCC GCACCGCCAT CGATGCAGAG 
TTCTCCCTCT TTAATGAGCC TCAAGGAAAA GAGCTGTTTG GCTTGGACAC TCTCCACAAG 
GTGTTGTGGA TCAAACTGTT GGAGGAAATG TTTCTGGGCA TGCCCAGTGA GTACCCGTGG 
GGCGATGAGA TGATGCTGTT CCTTAACGTC TTCAACGGGG CCCTTCTGCT GCACCCCGAG 
GACAGCTCCC TGCTGAGGCA GTACACGGCG ACCGCCATCA ACACCGCCGT TCACTTTAAC 
CATCTGTTCT CCCTGAGCGG GTACCAGTGG ATGCTGCCAA CCATGCTGCA GGTCTATGCA 
GACTACGAGA GCAACCCTCT TCTGAGGCAG GGCATTGAGT TTTGCTGCCG GCAGTTTTAC 
ATCCTCCATC GAAAACCCTT TATCCTGCAG CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG 
TTCACAACCA GCACCAGTAC CGGTCTTTCT AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC 
CTGCTGGTCT CTCTAGAAGG TGAAACCCAG GATGCCCTGG ATGCTCTGGA GCTGGTAAAA 
GCTGAGAAGC CTCTACGCTC TCTGGATTTC TGTTATGGTA ATGAGGACCT GACCTTTTCT 
ATCAGCGAGG CCATCAAGCT GTGCGTCACT GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG 
AGTCTCCAGA TGCTGATGGT GCTGGAGGCC TTGGTTCCCT GCTACCTCCA GAAACTGAAG 
AGCAACACAG TTACGATGGA GTCTGCTTCG GCTGCCAGAG ATGAGATTGC GGCCATCGCT 
GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC TACAGCTCTG AAACCCTCAC AAGGCCCATG 
ACGGCTCCCC AGATATCTCG CTGTGATCAG GGCCACAAGG GCGGCACTAC AGCTAACCAC 
GCCATGTCCG GAGGGGTCAA CACCAGGGAC AATCTTCACC TGCTGGAGGA GGGCCAGGGC 
ATGCCAAGGG AAGAGCTGGA CGAGCGCATT GCCAGGGAGG AGTTCCGCCG TCCACGGGAG 
TCCCTGTTGA ACATCTGCAC CGAGTTTTAC AAACACTGCG GGCCACGGCT CAAAATCCTA 
CAGAACGTTG CAGGCGAGCC GCGGGTCACA GCTCTGGAGC TACTGGACAT CAAGTCCCAC 
ATGAGGCTGG CAGAGATCGC CCACGCCCTG CTGAAGCTGG CGCCCTACGA CACCCTGACC 
ATGGAGAGCC GAGGGCTGCG GCGTTACATC ATGGAGATGC TGCCCATCAC CGACTGGTCG 
TCCGAGGCCG TCCGGCCTGC CCTCATCCTC ATCCTCAAGA GGCTGGACCG CATGTTCAAC 
AAGATCCACA AGATGCCCAC GCTCAGGTGC GCTCGCCCTC AGGCTTTATG CAGCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTCCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGACGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777712.1
CDS	398..10852
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922258.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922258.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGAAGAAGTG 
GTGAACCTGG CATCACGACG TCTGTCTGTC AGCCCATCCT GCGCCTCCAG CAACTCCCAC 
AGGAACTACT CTTTCCGCCG GGGCTCTGTA TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA 
GATGAAGAAA ACACAACAGA ACACACACCC ACTCACCACA TGCTACAGCC ACCTCAAGCT 
GTCTTCCCAG CATGCATCTG TGCTGCCGTC CTACCAATAG TGCACCTCAT GGAAGACGGC 
GAAGTTAGAG AGGATGGCGT GGCTGTGAGT GCTGTTGCCC AGCAAATCCT CTGGAACTGC 
CTCATTGAAG ATCCAGCTCT CGTTCTCCGC CACTTCCTGG AAAAGCTGAC AGTTAGCAAC 
AGACAGGACG AGCTGATGTA CATGCTTAGG AAGCTCCTGC TCAACATTGG AGATCTGCCA 
GCCCAGACCT CTCACATCCT TTTTAACTAC CTGGTGGGCC TGATCATGTA TTTTGTGCGG 
ACTCCCTGTG AATGGGGCAT GGACGCCATC TCGGCCACGC TGACCTTCCT CTGGGAGGTG 
GTCGGCTACG TGGAGGGGCT CTTCTTTAAA GACCTGAAGC AGACCATGAA GAAAGAGCAG 
TGTGAGGTCA AACTGCTGGT CACAGCATCG ATGCCAGGAA CCAAAACGCT GGTGGTGCAC 
GGGCAGAATG AATGTGACAT CCCAACCCAG CTACCAGTCC ACGAAGACAC TCAGTTTGAA 
GCCCTGCTAA AGGAGTGTCT GGAATTTTTC AACATTCCCG AGGCCAGATC GGCACACTAC 
TTCCTCATGG ATAAACGATG GAACCTCATC CATTATTCAA AGACATATGT TAGAGACATC 
TACCCGTTCC GAAGGTCAGT CTCTCCTCAG CTCAACCTGG TCCACATGTT GCCTGAGAAA 
GGACAGGAGC TCATCCAGAA ACAGGTGTTT TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC 
CTCTTCCTCA TCTCCCTCAC ACAACACATG CCAGCTGTGC ACAAGCAGTC CCACGTCTCC 
CTGCTGCAGG AGGACCTGCT TCGCCTGCCC TCCTTCCCCC GCACCGCCAT CGATGCAGAG 
TTCTCCCTCT TTAATGAGCC TCAAGGAAAA GAGCTGTTTG GCTTGGACAC TCTCCACAAG 
GTGTTGTGGA TCAAACTGTT GGAGGAAATG TTTCTGGGCA TGCCCAGTGA GTACCCGTGG 
GGCGATGAGA TGATGCTGTT CCTTAACGTC TTCAACGGGG CCCTTCTGCT GCACCCCGAG 
GACAGCTCCC TGCTGAGGCA GTACACGGCG ACCGCCATCA ACACCGCCGT TCACTTTAAC 
CATCTGTTCT CCCTGAGCGG GTACCAGTGG ATGCTGCCAA CCATGCTGCA GGTCTATGCA 
GACTACGAGA GCAACCCTCT TCTGAGGCAG GGCATTGAGT TTTGCTGCCG GCAGTTTTAC 
ATCCTCCATC GAAAACCCTT TATCCTGCAG CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG 
TTCACAACCA GCACCAGTAC CGGTCTTTCT AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC 
CTGCTGGTCT CTCTAGAAGG TGAAACCCAG GATGCCCTGG ATGCTCTGGA GCTGGTAAAA 
GCTGAGAAGC CTCTACGCTC TCTGGATTTC TGTTATGGTA ATGAGGACCT GACCTTTTCT 
ATCAGCGAGG CCATCAAGCT GTGCGTCACT GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG 
AGTCTCCAGA TGCTGATGGT GCTGGAGGCC TTGGTTCCCT GCTACCTCCA GAAACTGAAG 
AGCAACACAG TTACGATGGA GTCTGCTTCG GCTGCCAGAG ATGAGATTGC GGCCATCGCT 
GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC TACAGCTCTG AAACCCTCAC AAGGCCCATG 
ACGGCTCCCC AGATATCTCG CTGTGATCAG GGCCACAAGG GCGGCACTAC AGCTAACCAC 
GCCATGTCCG GAGGGGTCAA CACCAGGGAC AATCTTCACC TGCTGGAGGA GGGCCAGGGC 
ATGCCAAGGG AAGAGCTGGA CGAGCGCATT GCCAGGGAGG AGTTCCGCCG TCCACGGGAG 
TCCCTGTTGA ACATCTGCAC CGAGTTTTAC AAACACTGCG GGCCACGGCT CAAAATCCTA 
CAGAACGTTG CAGGCGAGCC GCGGGTCACA GCTCTGGAGC TACTGGACAT CAAGTCCCAC 
ATGAGGCTGG CAGAGATCGC CCACGCCCTG CTGAAGCTGG CGCCCTACGA CACCCTGACC 
ATGGAGAGCC GAGGGCTGCG GCGTTACATC ATGGAGATGC TGCCCATCAC CGACTGGTCG 
TCCGAGGCCG TCCGGCCTGC CCTCATCCTC ATCCTCAAGA GGCTGGACCG CATGTTCAAC 
AAGATCCACA AGATGCCCAC GCTCAGGTGC GCTCGCCCTC AGGCTTTATG CAGCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTCCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGACGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922259.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777713.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.

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CloneID	OMa09674
Clone ID Related Accession (Same CDS sequence)	XM_012922259.2
Accession Version	XM_012922259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGCGCATG GAGACCAAAA GAGGTCAGTC ACCTTTACAG AGAATCAAGG CCAGCAGGCA 
TCCACGGGGG CAAGCAAGCC TCCAACTCCC AGCACTGTGG ACCCACCGGG CGAAGGCAGA 
GGGACGAGTC CTGCCCCATC AAAGTCCCCA ACCCTGGAAG TACCCCCCAC CTCTTCAGCC 
ATGATCTTGG CTGACCTGCA CGGACCTACA AATGCACAGG TCCCTCAAGC ATTAAGCAGC 
AAGGAGAAGA GAGAGCAGTG GGGGCTACGC AGCAGCCTCT CCCCTCCCCC ATCCATCTCC 
CGGCCTTCCA CTCCAACACC CCCATCCCGA ACCTGCTCCC CTCTCCCCCT CTCCAGAACC 
TGCTCCCCTC TCCCTTTGTC CCGCACCTCC TCTCCTCTTC CACCGCCACT CCCAGTGCTG 
AGCACAGCAC CCGCTCCGGG TCCACCGCCG CCACCTCCAT TACCACCAAC TCATCTTGCT 
GCTGCTCCTC CTCCTCCACC ACCCCCGGGG CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC 
GAGGACACAA CCGCCCTGCT GCCGCGCAGC GACACCCTGC TGCACCTCAG CGAGGACGAC 
GGCACCGAGA ACCCGCTTCT CACCCCGCTG CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC 
CGCCGCTCTC CCCTCCCCCT CTCACCCGTC GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777713.1
CDS	398..10837
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922259.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922259.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGCGCATG GAGACCAAAA GAGGTCAGTC ACCTTTACAG AGAATCAAGG CCAGCAGGCA 
TCCACGGGGG CAAGCAAGCC TCCAACTCCC AGCACTGTGG ACCCACCGGG CGAAGGCAGA 
GGGACGAGTC CTGCCCCATC AAAGTCCCCA ACCCTGGAAG TACCCCCCAC CTCTTCAGCC 
ATGATCTTGG CTGACCTGCA CGGACCTACA AATGCACAGG TCCCTCAAGC ATTAAGCAGC 
AAGGAGAAGA GAGAGCAGTG GGGGCTACGC AGCAGCCTCT CCCCTCCCCC ATCCATCTCC 
CGGCCTTCCA CTCCAACACC CCCATCCCGA ACCTGCTCCC CTCTCCCCCT CTCCAGAACC 
TGCTCCCCTC TCCCTTTGTC CCGCACCTCC TCTCCTCTTC CACCGCCACT CCCAGTGCTG 
AGCACAGCAC CCGCTCCGGG TCCACCGCCG CCACCTCCAT TACCACCAAC TCATCTTGCT 
GCTGCTCCTC CTCCTCCACC ACCCCCGGGG CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC 
GAGGACACAA CCGCCCTGCT GCCGCGCAGC GACACCCTGC TGCACCTCAG CGAGGACGAC 
GGCACCGAGA ACCCGCTTCT CACCCCGCTG CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC 
CGCCGCTCTC CCCTCCCCCT CTCACCCGTC GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562580.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562637.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.

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CloneID	OMa09675
Clone ID Related Accession (Same CDS sequence)	XM_004562580.3
Accession Version	XM_004562580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGGGTGGCCA GTGTGCAGAG CGAACCAGGT 
CAGAACCTCC TGATCCAGCC CCCGTTAGGA AGAAAGCGAG GACTCAGACA GATGCGACGC 
CCCCTGCTGT CCATTCCAAA AACCGAACCA CGCGGTCGAT CTGGAGCGAG GCTTTCTACA 
ACGCGCAGGA GCATCCAGCC CAAGAACAAA CCACTGGAAA CTTTATTGGA CTGTGAAGCG 
CATGGAGACC AAAAGAGGTC AGTCACCTTT ACAGAGAATC AAGGCCAGCA GGCATCCACG 
GGGGCAAGCA AGCCTCCAAC TCCCAGCACT GTGGACCCAC CGGGCGAAGG CAGAGGGACG 
AGTCCTGCCC CATCAAAGTC CCCAACCCTG GAAGTACCCC CCACCTCTTC AGCCATGATC 
TTGGCTGACC TGCACGGACC TACAAATGCA CAGGTCCCTC AAGCATTAAG CAGCAAGGAG 
AAGAGAGAGC AGTGGGGGCT ACGCAGCAGC CTCTCCCCTC CCCCATCCAT CTCCCGGCCT 
TCCACTCCAA CACCCCCATC CCGAACCTGC TCCCCTCTCC CCCTCTCCAG AACCTGCTCC 
CCTCTCCCTT TGTCCCGCAC CTCCTCTCCT CTTCCACCGC CACTCCCAGT GCTGAGCACA 
GCACCCGCTC CGGGTCCACC GCCGCCACCT CCATTACCAC CAACTCATCT TGCTGCTGCT 
CCTCCTCCTC CACCACCCCC GGGGCCTTCT AGGATCAGAG AGAGCCCCGG GGACGAGGAC 
ACAACCGCCC TGCTGCCGCG CAGCGACACC CTGCTGCACC TCAGCGAGGA CGACGGCACC 
GAGAACCCGC TTCTCACCCC GCTGCTGTCC CCTCCTCCCC CCCGCCGGTC GCCCCGCCGC 
TCTCCCCTCC CCCTCTCACC CGTCGACCTG GACCTGGATG AGTCCCACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562637.1
CDS	399..10832
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562580.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562580.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGGGTGGCCA GTGTGCAGAG CGAACCAGGT 
CAGAACCTCC TGATCCAGCC CCCGTTAGGA AGAAAGCGAG GACTCAGACA GATGCGACGC 
CCCCTGCTGT CCATTCCAAA AACCGAACCA CGCGGTCGAT CTGGAGCGAG GCTTTCTACA 
ACGCGCAGGA GCATCCAGCC CAAGAACAAA CCACTGGAAA CTTTATTGGA CTGTGAAGCG 
CATGGAGACC AAAAGAGGTC AGTCACCTTT ACAGAGAATC AAGGCCAGCA GGCATCCACG 
GGGGCAAGCA AGCCTCCAAC TCCCAGCACT GTGGACCCAC CGGGCGAAGG CAGAGGGACG 
AGTCCTGCCC CATCAAAGTC CCCAACCCTG GAAGTACCCC CCACCTCTTC AGCCATGATC 
TTGGCTGACC TGCACGGACC TACAAATGCA CAGGTCCCTC AAGCATTAAG CAGCAAGGAG 
AAGAGAGAGC AGTGGGGGCT ACGCAGCAGC CTCTCCCCTC CCCCATCCAT CTCCCGGCCT 
TCCACTCCAA CACCCCCATC CCGAACCTGC TCCCCTCTCC CCCTCTCCAG AACCTGCTCC 
CCTCTCCCTT TGTCCCGCAC CTCCTCTCCT CTTCCACCGC CACTCCCAGT GCTGAGCACA 
GCACCCGCTC CGGGTCCACC GCCGCCACCT CCATTACCAC CAACTCATCT TGCTGCTGCT 
CCTCCTCCTC CACCACCCCC GGGGCCTTCT AGGATCAGAG AGAGCCCCGG GGACGAGGAC 
ACAACCGCCC TGCTGCCGCG CAGCGACACC CTGCTGCACC TCAGCGAGGA CGACGGCACC 
GAGAACCCGC TTCTCACCCC GCTGCTGTCC CCTCCTCCCC CCCGCCGGTC GCCCCGCCGC 
TCTCCCCTCC CCCTCTCACC CGTCGACCTG GACCTGGATG AGTCCCACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562581.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562638.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.

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CloneID	OMa09676
Clone ID Related Accession (Same CDS sequence)	XM_004562581.3
Accession Version	XM_004562581.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562638.1
CDS	399..10829
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562581.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562581.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922261.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777715.1 Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X11, mRNA.

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CloneID	OMa09677
Clone ID Related Accession (Same CDS sequence)	XM_012922261.2 , XM_012922261.3
Accession Version	XM_012922261.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	protein unc-80 homolog isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GACGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777715.1
CDS	398..10780
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922261.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922261.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GACGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922261.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777715.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.

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CloneID	OMa09677
Clone ID Related Accession (Same CDS sequence)	XM_012922261.2 , XM_012922261.3
Accession Version	XM_012922261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012922261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GACGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777715.1
CDS	399..10781
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922261.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922261.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GACGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562584.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562641.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.

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CloneID	OMa09678
Clone ID Related Accession (Same CDS sequence)	XM_004562584.3
Accession Version	XM_004562584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004562584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562641.1
CDS	398..10768
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562584.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562584.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGCGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTACAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTC 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA CGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922262.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777716.1 Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X13, mRNA.

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CloneID	OMa09679
Clone ID Related Accession (Same CDS sequence)	XM_012922262.2 , XM_012922262.3
Accession Version	XM_012922262.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	protein unc-80 homolog isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777716.1
CDS	398..10678
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922262.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra unc-80 homolog (C. elegans) (unc80), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922262.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922262.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777716.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.

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CloneID	OMa09679
Clone ID Related Accession (Same CDS sequence)	XM_012922262.2 , XM_012922262.3
Accession Version	XM_012922262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012922262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777716.1
CDS	399..10679
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922262.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922262.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGCGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTACAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTC CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA CGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922263.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777717.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.

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CloneID	OMa09680
Clone ID Related Accession (Same CDS sequence)	XM_012922263.2
Accession Version	XM_012922263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTAAATC CTTCTCTGCG CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC 
GAGCACATCC TCAAGAACCT GCAGCAGGAG GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC 
AGCATCATCA CAGCCATCCC TGTGGCTCAG GAGGCCTGCT ACGAACCCAC ATGCAGCCCT 
CCACCCGAGC AAGAGGAGGA AGAAGAAGTG GTGAACCTGG CATCACGACG TCTGTCTGTC 
AGCCCATCCT GCGCCTCCAG CAACTCCCAC AGGAACTACT CTTTCCGCCG GGGCTCTGTA 
TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA GATGAAGAAA ACACAACAGA ACACACACCC 
ACTCACCACA TGCTACAGCC ACCTCAAGCT GTCTTCCCAG CATGCATCTG TGCTGCCGTC 
CTACCAATAG TGCACCTCAT GGAAGACGGC GAAGTTAGAG AGGATGGCGT GGCTGTGAGT 
GCTGTTGCCC AGCAAATCCT CTGGAACTGC CTCATTGAAG ATCCAGCTCT CGTTCTCCGC 
CACTTCCTGG AAAAGCTGAC AGTTAGCAAC AGACAGGACG AGCTGATGTA CATGCTTAGG 
AAGCTCCTGC TCAACATTGG AGATCTGCCA GCCCAGACCT CTCACATCCT TTTTAACTAC 
CTGGTGGGCC TGATCATGTA TTTTGTGCGG ACTCCCTGTG AATGGGGCAT GGACGCCATC 
TCGGCCACGC TGACCTTCCT CTGGGAGGTG GTCGGCTACG TGGAGGGGCT CTTCTTTAAA 
GACCTGAAGC AGACCATGAA GAAAGAGCAG TGTGAGGTCA AACTGCTGGT CACAGCATCG 
ATGCCAGGAA CCAAAACGCT GGTGGTGCAC GGGCAGAATG AATGTGACAT CCCAACCCAG 
CTACCAGTCC ACGAAGACAC TCAGTTTGAA GCCCTGCTAA AGGAGTGTCT GGAATTTTTC 
AACATTCCCG AGGCCAGATC GGCACACTAC TTCCTCATGG ATAAACGATG GAACCTCATC 
CATTATTCAA AGACATATGT TAGAGACATC TACCCGTTCC GAAGGTCAGT CTCTCCTCAG 
CTCAACCTGG TCCACATGTT GCCTGAGAAA GGACAGGAGC TCATCCAGAA ACAGGTGTTT 
TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC CTCTTCCTCA TCTCCCTCAC ACAACACATG 
CCAGCTGTGC ACAAGCAGTC CCACGTCTCC CTGCTGCAGG AGGACCTGCT TCGCCTGCCC 
TCCTTCCCCC GCACCGCCAT CGATGCAGAG TTCTCCCTCT TTAATGAGCC TCAAGGAAAA 
GAGCTGTTTG GCTTGGACAC TCTCCACAAG GTGTTGTGGA TCAAACTGTT GGAGGAAATG 
TTTCTGGGCA TGCCCAGTGA GTACCCGTGG GGCGATGAGA TGATGCTGTT CCTTAACGTC 
TTCAACGGGG CCCTTCTGCT GCACCCCGAG GACAGCTCCC TGCTGAGGCA GTACACGGCG 
ACCGCCATCA ACACCGCCGT TCACTTTAAC CATCTGTTCT CCCTGAGCGG GTACCAGTGG 
ATGCTGCCAA CCATGCTGCA GGTCTATGCA GACTACGAGA GCAACCCTCT TCTGAGGCAG 
GGCATTGAGT TTTGCTGCCG GCAGTTTTAC ATCCTCCATC GAAAACCCTT TATCCTGCAG 
CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG TTCACAACCA GCACCAGTAC CGGTCTTTCT 
AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC CTGCTGGTCT CTCTAGAAGG TGAAACCCAG 
GATGCCCTGG ATGCTCTGGA GCTGGTAAAA GCTGAGAAGC CTCTACGCTC TCTGGATTTC 
TGTTATGGTA ATGAGGACCT GACCTTTTCT ATCAGCGAGG CCATCAAGCT GTGCGTCACT 
GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG AGTCTCCAGA TGCTGATGGT GCTGGAGGCC 
TTGGTTCCCT GCTACCTCCA GAAACTGAAG AGCAACACAG TTACGATGGA GTCTGCTTCG 
GCTGCCAGAG ATGAGATTGC GGCCATCGCT GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC 
TACAGCTCTG AAACCCTCAC AAGGCCCATG ACGGCTCCCC AGATATCTCG CTGTGATCAG 
GGCCACAAGG GCGGCACTAC AGCTAACCAC GCCATGTCCG GAGGGGTCAA CACCAGGGAC 
AATCTTCACC TGCTGGAGGA GGGCCAGGGC ATGCCAAGGG AAGAGCTGGA CGAGCGCATT 
GCCAGGGAGG AGTTCCGCCG TCCACGGGAG TCCCTGTTGA ACATCTGCAC CGAGTTTTAC 
AAACACTGCG GGCCACGGCT CAAAATCCTA CAGAACGTTG CAGGCGAGCC GCGGGTCACA 
GCTCTGGAGC TACTGGACAT CAAGTCCCAC ATGAGGCTGG CAGAGATCGC CCACGCCCTG 
CTGAAGCTGG CGCCCTACGA CACCCTGACC ATGGAGAGCC GAGGGCTGCG GCGTTACATC 
ATGGAGATGC TGCCCATCAC CGACTGGTCG TCCGAGGCCG TCCGGCCTGC CCTCATCCTC 
ATCCTCAAGA GGCTGGACCG CATGTTCAAC AAGATCCACA AGATGCCCAC GCTCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTCCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGACGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777717.1
CDS	398..10612
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922263.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922263.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TACTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTAAATC CTTCTCTGCG CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC 
GAGCACATCC TCAAGAACCT GCAGCAGGAG GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC 
AGCATCATCA CAGCCATCCC TGTGGCTCAG GAGGCCTGCT ACGAACCCAC ATGCAGCCCT 
CCACCCGAGC AAGAGGAGGA AGAAGAAGTG GTGAACCTGG CATCACGACG TCTGTCTGTC 
AGCCCATCCT GCGCCTCCAG CAACTCCCAC AGGAACTACT CTTTCCGCCG GGGCTCTGTA 
TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA GATGAAGAAA ACACAACAGA ACACACACCC 
ACTCACCACA TGCTACAGCC ACCTCAAGCT GTCTTCCCAG CATGCATCTG TGCTGCCGTC 
CTACCAATAG TGCACCTCAT GGAAGACGGC GAAGTTAGAG AGGATGGCGT GGCTGTGAGT 
GCTGTTGCCC AGCAAATCCT CTGGAACTGC CTCATTGAAG ATCCAGCTCT CGTTCTCCGC 
CACTTCCTGG AAAAGCTGAC AGTTAGCAAC AGACAGGACG AGCTGATGTA CATGCTTAGG 
AAGCTCCTGC TCAACATTGG AGATCTGCCA GCCCAGACCT CTCACATCCT TTTTAACTAC 
CTGGTGGGCC TGATCATGTA TTTTGTGCGG ACTCCCTGTG AATGGGGCAT GGACGCCATC 
TCGGCCACGC TGACCTTCCT CTGGGAGGTG GTCGGCTACG TGGAGGGGCT CTTCTTTAAA 
GACCTGAAGC AGACCATGAA GAAAGAGCAG TGTGAGGTCA AACTGCTGGT CACAGCATCG 
ATGCCAGGAA CCAAAACGCT GGTGGTGCAC GGGCAGAATG AATGTGACAT CCCAACCCAG 
CTACCAGTCC ACGAAGACAC TCAGTTTGAA GCCCTGCTAA AGGAGTGTCT GGAATTTTTC 
AACATTCCCG AGGCCAGATC GGCACACTAC TTCCTCATGG ATAAACGATG GAACCTCATC 
CATTATTCAA AGACATATGT TAGAGACATC TACCCGTTCC GAAGGTCAGT CTCTCCTCAG 
CTCAACCTGG TCCACATGTT GCCTGAGAAA GGACAGGAGC TCATCCAGAA ACAGGTGTTT 
TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC CTCTTCCTCA TCTCCCTCAC ACAACACATG 
CCAGCTGTGC ACAAGCAGTC CCACGTCTCC CTGCTGCAGG AGGACCTGCT TCGCCTGCCC 
TCCTTCCCCC GCACCGCCAT CGATGCAGAG TTCTCCCTCT TTAATGAGCC TCAAGGAAAA 
GAGCTGTTTG GCTTGGACAC TCTCCACAAG GTGTTGTGGA TCAAACTGTT GGAGGAAATG 
TTTCTGGGCA TGCCCAGTGA GTACCCGTGG GGCGATGAGA TGATGCTGTT CCTTAACGTC 
TTCAACGGGG CCCTTCTGCT GCACCCCGAG GACAGCTCCC TGCTGAGGCA GTACACGGCG 
ACCGCCATCA ACACCGCCGT TCACTTTAAC CATCTGTTCT CCCTGAGCGG GTACCAGTGG 
ATGCTGCCAA CCATGCTGCA GGTCTATGCA GACTACGAGA GCAACCCTCT TCTGAGGCAG 
GGCATTGAGT TTTGCTGCCG GCAGTTTTAC ATCCTCCATC GAAAACCCTT TATCCTGCAG 
CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG TTCACAACCA GCACCAGTAC CGGTCTTTCT 
AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC CTGCTGGTCT CTCTAGAAGG TGAAACCCAG 
GATGCCCTGG ATGCTCTGGA GCTGGTAAAA GCTGAGAAGC CTCTACGCTC TCTGGATTTC 
TGTTATGGTA ATGAGGACCT GACCTTTTCT ATCAGCGAGG CCATCAAGCT GTGCGTCACT 
GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG AGTCTCCAGA TGCTGATGGT GCTGGAGGCC 
TTGGTTCCCT GCTACCTCCA GAAACTGAAG AGCAACACAG TTACGATGGA GTCTGCTTCG 
GCTGCCAGAG ATGAGATTGC GGCCATCGCT GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC 
TACAGCTCTG AAACCCTCAC AAGGCCCATG ACGGCTCCCC AGATATCTCG CTGTGATCAG 
GGCCACAAGG GCGGCACTAC AGCTAACCAC GCCATGTCCG GAGGGGTCAA CACCAGGGAC 
AATCTTCACC TGCTGGAGGA GGGCCAGGGC ATGCCAAGGG AAGAGCTGGA CGAGCGCATT 
GCCAGGGAGG AGTTCCGCCG TCCACGGGAG TCCCTGTTGA ACATCTGCAC CGAGTTTTAC 
AAACACTGCG GGCCACGGCT CAAAATCCTA CAGAACGTTG CAGGCGAGCC GCGGGTCACA 
GCTCTGGAGC TACTGGACAT CAAGTCCCAC ATGAGGCTGG CAGAGATCGC CCACGCCCTG 
CTGAAGCTGG CGCCCTACGA CACCCTGACC ATGGAGAGCC GAGGGCTGCG GCGTTACATC 
ATGGAGATGC TGCCCATCAC CGACTGGTCG TCCGAGGCCG TCCGGCCTGC CCTCATCCTC 
ATCCTCAAGA GGCTGGACCG CATGTTCAAC AAGATCCACA AGATGCCCAC GCTCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTCCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGACGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922264.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777718.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.

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CloneID	OMa09681
Clone ID Related Accession (Same CDS sequence)	XM_012922264.2
Accession Version	XM_012922264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GCGCATGGAG ACCAAAAGAG GTCAGTCACC 
TTTACAGAGA ATCAAGGCCA GCAGGCATCC ACGGGGGCAA GCAAGCCTCC AACTCCCAGC 
ACTGTGGACC CACCGGGCGA AGGCAGAGGG ACGAGTCCTG CCCCATCAAA GTCCCCAACC 
CTGGAAGTAC CCCCCACCTC TTCAGCCATG ATCTTGGCTG ACCTGCACGG ACCTACAAAT 
GCACAGGTCC CTCAAGCATT AAGCAGCAAG GAGAAGAGAG AGCAGTGGGG GCTACGCAGC 
AGCCTCTCCC CTCCCCCATC CATCTCCCGG CCTTCCACTC CAACACCCCC ATCCCGAACC 
TGCTCCCCTC TCCCCCTCTC CAGAACCTGC TCCCCTCTCC CTTTGTCCCG CACCTCCTCT 
CCTCTTCCAC CGCCACTCCC AGTGCTGAGC ACAGCACCCG CTCCGGGTCC ACCGCCGCCA 
CCTCCATTAC CACCAACTCA TCTTGCTGCT GCTCCTCCTC CTCCACCACC CCCGGGGCCT 
TCTAGGATCA GAGAGAGCCC CGGGGACGAG GACACAACCG CCCTGCTGCC GCGCAGCGAC 
ACCCTGCTGC ACCTCAGCGA GGACGACGGC ACCGAGAACC CGCTTCTCAC CCCGCTGCTG 
TCCCCTCCTC CCCCCCGCCG GTCGCCCCGC CGCTCTCCCC TCCCCCTCTC ACCCGTCGAC 
CTGGACCTGG ATGAGTCCCA CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777718.1
CDS	398..10624
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922264.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922264.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GCGCATGGAG ACCAAAAGAG GTCAGTCACC 
TTTACAGAGA ATCAAGGCCA GCAGGCATCC ACGGGGGCAA GCAAGCCTCC AACTCCCAGC 
ACTGTGGACC CACCGGGCGA AGGCAGAGGG ACGAGTCCTG CCCCATCAAA GTCCCCAACC 
CTGGAAGTAC CCCCCACCTC TTCAGCCATG ATCTTGGCTG ACCTGCACGG ACCTACAAAT 
GCACAGGTCC CTCAAGCATT AAGCAGCAAG GAGAAGAGAG AGCAGTGGGG GCTACGCAGC 
AGCCTCTCCC CTCCCCCATC CATCTCCCGG CCTTCCACTC CAACACCCCC ATCCCGAACC 
TGCTCCCCTC TCCCCCTCTC CAGAACCTGC TCCCCTCTCC CTTTGTCCCG CACCTCCTCT 
CCTCTTCCAC CGCCACTCCC AGTGCTGAGC ACAGCACCCG CTCCGGGTCC ACCGCCGCCA 
CCTCCATTAC CACCAACTCA TCTTGCTGCT GCTCCTCCTC CTCCACCACC CCCGGGGCCT 
TCTAGGATCA GAGAGAGCCC CGGGGACGAG GACACAACCG CCCTGCTGCC GCGCAGCGAC 
ACCCTGCTGC ACCTCAGCGA GGACGACGGC ACCGAGAACC CGCTTCTCAC CCCGCTGCTG 
TCCCCTCCTC CCCCCCGCCG GTCGCCCCGC CGCTCTCCCC TCCCCCTCTC ACCCGTCGAC 
CTGGACCTGG ATGAGTCCCA CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922265.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777719.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.

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CloneID	OMa09682
Clone ID Related Accession (Same CDS sequence)	XM_012922265.2
Accession Version	XM_012922265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012922265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAC GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777719.1
CDS	398..10567
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922265.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922265.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGCGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TACTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGCGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTACAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTCCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAC GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_023153469.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009237.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.

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CloneID	OMa313319
Clone ID Related Accession (Same CDS sequence)	XM_023153469.1
Accession Version	XM_023153469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGACA AGGCGGGCTT CGGAAACAAC 
TTCGCCACAG GAGACAACAA ATCTGTAGCC CAGAATATGG AGGCCGTGGT GGTGGGCTGC 
ATGTTCAAGT CCCTCATCAC CCGCTGCGCC TCAACCACAC ATGAGCTGCA CAGCCCCGAG 
AACCTGGGTC TGTACTGTGA CATCCGCCAG CTGGTCCAGT TCATTAAAGA GGCCCACGGA 
AACGTGTTCC GCAGGGTGGC ACTGAGCGCG CTCCTGGACA GCGCGGAGAA GGTCACCGCC 
ACCAAGAAAC CTGATGAGAA GGAGGAGACC AAACAGCCCG GTCCCAGGAG GTCAGAGGCG 
GGTGTGTCCG GGGAGAAGGG TCAGGTGTCC AGTACTGCAG ACGAGTGTCG CAGCTACATC 
TCCAGTAGAC CCCCCCAGAC ACCCGAACAT GAAGAACAGA TCCCGGGTGC TCTGCTGGGC 
AGGAAGGATT TTTGGAGGAA GATGTTCAAG TCGCAGAGCG CCGCCAGTGA CACCAGCAGC 
CAGTCGGAAC AAGACACCTC AGAGTGCACC ACCGCACACT CTGGCACCAC CACCGACCGC 
CGCTCACGAT CCAGATCCCG TCGCATTTCG CTTCGCAAGA AGTTGAAGCT GCCTATCGGG 
AACTGGTTGA AGCGCTCCTC TCTCTCTGGA CTGACTGACG GCGTCGAGGA CTTGCTGGAT 
ATCAGCTCGG TGGATCGACT CTCCTTCATA CGTCAGAGTT CAAAGGTGAA GTTCACTAGT 
GCAGTGAAGC TATCTGAAGG TGGCGCTGTA GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG 
AATTTCTTCA AGCGGCTCGG TAAATGGAGG TCTGGCAGGA GGAATCCATC CAAGCTTCAC 
CACACAGAAG AGAAAGATGG ACCTCACGGT TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG 
GCCATGAAGA ACAAGAATGT AGTGAATCTG GGCGCCATCC GACAGGGTAT GAAACGCTTC 
CAGTTTCTCC TGAACTGCTG CGAGCCAGGA ACCATACCAG ATGCCTCCAT CCTCGCCGCT 
GCTCTGGACC TCGAAGCCCC AGTTGTGGCC AGGGCCTCCC TTTTCTTGGA ATGCGCCAGA 
TTTGTCCACC GGTGTAATCG TGGCAACTGG CCCGAATGGA TGAAGGGCCA CCACGTAAAC 
ATCACCAAGA GAGGGCTGTC CAGGGGCCGA TCGCCTATTG TGGGCAATAA AAGAAACCAG 
AAGCTCCAGT GGAACGCTGC CAAGCATTTC TGCCAGTGGG GAGACGCGAT TGGCACGAGG 
CTCAGCGAAC TCTGCCACTC GGACAGTGAG AGCCCCGCCA ACATCCTGGG TTATATTTAC 
GATGAAGAGA CCAAACGCAG GATGAGGAAA GAAGACGAGG AAGAAGACTA TTTAGATGAC 
AACACGGTCA ATCCTACAAA ATGTGGCTGC CCCTTCGCAC TGAAGATGGC CGCTTGCCAG 
CTGTTGTTGG AGATCACCAC TTTCCTGCGA GAGACCTTCC CCTGCTTACC ACGACCGCGC 
ACCGAACCGC TTGTGGATCT GGACAGCTGC CGCCTGCGTC TGGACCCAGA GCTCGGCCGC 
CATCGCTACG AGAGGAAGAT CAGCTTTGCG GGCATCCTCG ACGACGAAGA CGGGCACGAT 
TCCCTCAACA GCAGCAGCCA CACTCTGAAG TCTGACACCA CCTGTGATGA TAAGAAGACC 
CAGGAGGCTC AGGACAATCT CTCCCCACTC CCCTGCTCTC CAGCACCAGT GCGGAAGATT 
CGTATCGGAG GCTCGCGCCT GCTTCAGATC AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG 
GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG GGGAGTCTGA AGAGCACCAA GCTGTCTCGG 
CAGGACTCTG AGTCTGAGAA CGAAGAAGGC CTGCTGTCAC AAACGCAGAG CAGGGACACA 
GTTACTGACA TCGGCAGTCC CTTCAGCACC AGTGAACCCA GCATCGAGCC AGAAGGTCAG 
AGCTCAGGAG GGACGGAGGA CAACTACCAC CGCAACATGT CCTGGCTTCA CATTATGATC 
CTACTGTGCA ACCAGCAGAG TTTCATCTGT ACCCACATAG ACTTCTGCCA CCCACGCTGC 
TACCAACACC ACAGCCGCTC CTGCGCCCGT CTGGTCCGTG CCATCAAGCT CGTGTACGGG 
GAGACGGTGG ACAGCCTGAG GGAGGACAGC GCCTCCTCTG GTAACATCGG AGGACGTGTG 
AAGAAAAACA AAGAGTGTTC AGACAAGTCT TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA 
CCCACTGACT CCAACACAGA AGGGAAGAAG GATACAGGCA TGCTCAAGTA CATCCGCAAT 
CAGGTGATGA GTTTGTCACC AGCTCCTCTT TCACTGCTGA TCAAAGCAGC TCCTATCTTG 
ACTGACGACA TGTACGGAGA CATCCAGCCC GCAGCCTGGG AACTCCTACT CAGTGTGGAT 
GAACATATGG CAGCCGCTGC TGCTGCCTTG TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT 
GCAGTGACCG AAATGATGAT GGCAGAGTTC CAGCACCCTG AGGCCTGCCA GCGCATTAAC 
TCCATCCTGA AGTTTTACAC TCTGTGGCGT TTTCGCTACC AAGTGTGGCC TCGCATGGAG 
GAAGGAGCCC AGCAGATCTT TAAGATCCCT CCACCCAGCA TTAACTTCAC TCTCCCTTCT 
CCAATACTGG GCATGCCCTG TGTCCCCATA TTTGACCCTC CCTGGGTGCC CCAGAACACT 
GGCAGCGTCC AGGATCCAAT CAACGAAGAC CAGTCTTACC CCACATTAAT TCAGTCAGTC 
CATGCCATAA AATCCTTCTC TGCGCGGGCG GTGTCGCGCT CCCACCAGCG AGCCGAGCAC 
ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG AAGCGGCGTC TGGGCCGCGA GGCCAGCATC 
ATCACAGCCA TCCCTGTGGC TCAGGAGGCC TGCTACGAAC CCACATGCAG CCCTCCACCC 
GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG AACCTGGCAT CACGACGTCT GTCTGTCAGC 
CCATCCTGCG CCTCCAGCAA CTCCCACAGG AACTACTCTT TCCGCCGGGG CTCTGTATGG 
TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT GAAGAAAACA CAACAGAACA CACACCCACT 
CACCACATGC TACAGCCACC TCAAGCTGTC TTCCCAGCAT GCATCTGTGC TGCCGTCCTA 
CCAATAGTGC ACCTCATGGA AGACGGCGAA GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT 
GTTGCCCAGC AAATCCTCTG GAACTGCCTC ATTGAAGATC CAGCTCTCGT TCTCCGCCAC 
TTCCTGGAAA AGCTGACAGT TAGCAACAGA CAGGACGAGC TGATGTACAT GCTTAGGAAG 
CTCCTGCTCA ACATTGGAGA TCTGCCAGCC CAGACCTCTC ACATCCTTTT TAACTACCTG 
GTGGGCCTGA TCATGTATTT TGTGCGGACT CCCTGTGAAT GGGGCATGGA CGCCATCTCG 
GCCACGCTGA CCTTCCTCTG GGAGGTGGTC GGCTACGTGG AGGGGCTCTT CTTTAAAGAC 
CTGAAGCAGA CCATGAAGAA AGAGCAGTGT GAGGTCAAAC TGCTGGTCAC AGCATCGATG 
CCAGGAACCA AAACGCTGGT GGTGCACGGG CAGAATGAAT GTGACATCCC AACCCAGCTA 
CCAGTCCACG AAGACACTCA GTTTGAAGCC CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC 
ATTCCCGAGG CCAGATCGGC ACACTACTTC CTCATGGATA AACGATGGAA CCTCATCCAT 
TATTCAAAGA CATATGTTAG AGACATCTAC CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC 
AACCTGGTCC ACATGTTGCC TGAGAAAGGA CAGGAGCTCA TCCAGAAACA GGTGTTTTCC 
CGTAAACTAG AAGAAGTCGG TCGAGTCCTC TTCCTCATCT CCCTCACACA ACACATGCCA 
GCTGTGCACA AGCAGTCCCA CGTCTCCCTG CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC 
TTCCCCCGCA CCGCCATCGA TGCAGAGTTC TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG 
CTGTTTGGCT TGGACACTCT CCACAAGGTG TTGTGGATCA AACTGTTGGA GGAAATGTTT 
CTGGGCATGC CCAGTGAGTA CCCGTGGGGC GATGAGATGA TGCTGTTCCT TAACGTCTTC 
AACGGGGCCC TTCTGCTGCA CCCCGAGGAC AGCTCCCTGC TGAGGCAGTA CACGGCGACC 
GCCATCAACA CCGCCGTTCA CTTTAACCAT CTGTTCTCCC TGAGCGGGTA CCAGTGGATG 
CTGCCAACCA TGCTGCAGGT CTATGCAGAC TACGAGAGCA ACCCTCTTCT GAGGCAGGGC 
ATTGAGTTTT GCTGCCGGCA GTTTTACATC CTCCATCGAA AACCCTTTAT CCTGCAGCTG 
TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC ACAACCAGCA CCAGTACCGG TCTTTCTAAA 
GGAGTTTCGG CCCAGTGTCT GTTCGACCTG CTGGTCTCTC TAGAAGGTGA AACCCAGGAT 
GCCCTGGATG CTCTGGAGCT GGTAAAAGCT GAGAAGCCTC TACGCTCTCT GGATTTCTGT 
TATGGTAATG AGGACCTGAC CTTTTCTATC AGCGAGGCCA TCAAGCTGTG CGTCACTGTG 
GTTGCATATG CCCCCGAATC CTTCAGGAGT CTCCAGATGC TGATGGTGCT GGAGGCCTTG 
GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC AACACAGTTA CGATGGAGTC TGCTTCGGCT 
GCCAGAGATG AGATTGCGGC CATCGCTGCC CTAGCTACGT CTCTGCAGGC CCTGCTCTAC 
AGCTCTGAAA CCCTCACAAG GCCCATGACG GCTCCCCAGA TATCTCGCTG TGATCAGGGC 
CACAAGGGCG GCACTACAGC TAACCACGCC ATGTCCGGAG GGGTCAACAC CAGGGACAAT 
CTTCACCTGC TGGAGGAGGG CCAGGGCATG CCAAGGGAAG AGCTGGACGA GCGCATTGCC 
AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC CTGTTGAACA TCTGCACCGA GTTTTACAAA 
CACTGCGGGC CACGGCTCAA AATCCTACAG AACGTTGCAG GCGAGCCGCG GGTCACAGCT 
CTGGAGCTAC TGGACATCAA GTCCCACATG AGGCTGGCAG AGATCGCCCA CGCCCTGCTG 
AAGCTGGCGC CCTACGACAC CCTGACCATG GAGAGCCGAG GGCTGCGGCG TTACATCATG 
GAGATGCTGC CCATCACCGA CTGGTCGTCC GAGGCCGTCC GGCCTGCCCT CATCCTCATC 
CTCAAGAGGC TGGACCGCAT GTTCAACAAG ATCCACAAGA TGCCCACGCT CAGGTGCGCT 
CGCCCTCAGG CTTTATGCAG CAGGAGACAG GTGGAGTGGG AGGCGGCCAG TAGCCTGATC 
GAGGGGATCT GCCTGACCTT GCAGCGACAG CCGATCATCT CGTTCCTGCC TCACCTGCGC 
TCGCTTATCA ACGTCTGCGT CAACCTGGTG ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG 
GACGGGCTGC CACTCCTCCA CCTCAGCCCT TACCTGTCTC CGCCTCTGCC CTTTAGCACC 
GCCGTGGTCC GATTGGTTGC TCTGCAGATT CAGGCCTTGA AGGAAGACTT CCCCCTCAGT 
CACGTGATCT CACCTTTCAC CAATCAGGAA AGGCGGGAGG GGATGCTGCT CAACCTGCTC 
ATTCCCTTCG TGTTAACTGT AGGCTCCGGG AGCAAAGACA GCCCCCACCT GGAGCAGCCG 
GAGGTCTTCC TGCTCCTACA GACAGTCATC AACATCCTGC TTCCCCCTCG TATCATCTCC 
ACATCCCGCA CCAAGAACTT TATGCTTGAC GCCTCTCCGG CTCACTGCTC CACGCCGGGT 
GACCCTGGGA AAGATCTGCG TAGGGAGGGA TTAGCAGAGT CCACGAGCCA GGCTGCTTAT 
TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC GAGCGCTCGC TGGGGAACCA GTGGTATCGG 
CTGAGCCTGC AGGTGAAGGA GATGGCTCTG AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC 
TTCATCGACT TTATCGTCCG AACCCGGATC CCTATTTTTG TCCTGCTCAG ACCCTTTATA 
CAGTGCAAGT TGCTGACCCA GCCGGCCGAT TCCCAGGAAG AGATCACAGC GCGCCACCAC 
ATCGCTGATC AGCTGGAGCG GCGCTTCATC CCACGACCTC TCTGCAAGAG CTCACTGTTT 
GCAGAGTTCA ACAATGAGCT CAAGATCCTC AAAGAGGCTG TGCACAGCGG CTCAGCATAT 
CAGGGCAAGA CATCCATCAG CACGGTGGGC ACCTCCACGT CAGCCTACCG CCTCAGTTTG 
GCCACAATGT CGCGATCCAA CACAGGCACT GGAACAGTTT GGGAGCAGGA CAGCCAACCG 
TCACGGCAGC CCTCACAGGA CACGCTCAGC CGTACCGACG AGGACGATGA AGAGATTGAC 
TCCATGAGCA TTCCCAGCGT GGTGAGCGAG CACGAAGCCT TCATGCCCAG GGTGATGTCA 
CAGCGCCGTT TCTCCAGCCA CGCCACTGGC TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC 
ACCACCATGT TGCCCAGCCA CAGTGAACCC AATGTGCTAG ATGAGTCCCA GGGTCTTCTG 
CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT GTGCAGAGCG AACCAGGTCA GAACCTCCTG 
ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA CTCAGACAGA TGCGACGCCC CCTGCTGTCC 
ATTCCAAAAA CCGAACCACG CGGTCGATCT GGAGCGAGGC TTTCTACAAC GCGCAGGAGC 
ATCCAGCCCA AGAACAAACC ACTGGAAACT TTATTGGACT GTGAAGCGCA TGGAGACCAA 
AAGAGGTCAG TCACCTTTAC AGAGAATCAA GGCCAGCAGG CATCCACGGG GGCAAGCAAG 
CCTCCAACTC CCAGCACTGT GGACCCACCG GGCGAAGGCA GAGGGACGAG TCCTGCCCCA 
TCAAAGTCCC CAACCCTGGA AGTACCCCCC ACCTCTTCAG CCATGATCTT GGCTGACCTG 
CACGGACCTA CAAATGCACA GGTCCCTCAA GCATTAAGCA GCAAGGAGAA GAGAGAGCAG 
TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC CCATCCATCT CCCGGCCTTC CACTCCAACA 
CCCCCATCCC GAACCTGCTC CCCTCTCCCC CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG 
TCCCGCACCT CCTCTCCTCT TCCACCGCCA CTCCCAGTGC TGAGCACAGC ACCCGCTCCG 
GGTCCACCGC CGCCACCTCC ATTACCACCA ACTCATCTTG CTGCTGCTCC TCCTCCTCCA 
CCACCCCCGG GGCCTTCTAG GATCAGAGAG AGCCCCGGGG ACGAGGACAC AACCGCCCTG 
CTGCCGCGCA GCGACACCCT GCTGCACCTC AGCGAGGACG ACGGCACCGA GAACCCGCTT 
CTCACCCCGC TGCTGTCCCC TCCTCCCCCC CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC 
CTCTCACCCG TCGACCTGGA CCTGGATGAG TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009237.1
CDS	398..10819
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_023153469.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153469.1

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGACA AGGCGGGCTT CGGAAACAAC 
TTCGCCACAG GAGACAACAA ATCTGTAGCC CAGAATATGG AGGCCGTGGT GGTGGGCTGC 
ATGTTCAAGT CCCTCATCAC CCGCTGCGCC TCAACCACAC ATGAGCTGCA CAGCCCCGAG 
AACCTGGGTC TGTACTGTGA CATCCGCCAG CTGGTCCAGT TCATTAAAGA GGCCCACGGA 
AACGTGTTCC GCAGGGTGGC ACTGAGCGCG CTCCTGGACA GCGCGGAGAA GGTCACCGCC 
ACCAAGAAAC CTGATGAGAA GGAGGAGACC AAACAGCCCG GTCCCAGGAG GTCAGAGGCG 
GGTGTGTCCG GGGAGAAGGG TCAGGTGTCC AGTACTGCAG ACGAGTGTCG CAGCTACATC 
TCCAGTAGAC CCCCCCAGAC ACCCGAACAT GAAGAACAGA TCCCGGGTGC TCTGCTGGGC 
AGGAAGGATT TTTGGAGGAA GATGTTCAAG TCGCAGAGCG CCGCCAGTGA CACCAGCAGC 
CAGTCGGAAC AAGACACCTC AGAGTGCACC ACCGCACACT CTGGCACCAC CACCGACCGC 
CGCTCACGAT CCAGATCCCG TCGCATTTCG CTTCGCAAGA AGTTGAAGCT GCCTATCGGG 
AACTGGTTGA AGCGCTCCTC TCTCTCTGGA CTGACTGACG GCGTCGAGGA CTTGCTGGAT 
ATCAGCTCGG TGGATCGACT CTCCTTCATA CGTCAGAGTT CAAAGGTGAA GTTCACTAGT 
GCAGTGAAGC TATCTGAAGG TGGCGCTGTA GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG 
AATTTCTTCA AGCGGCTCGG TAAATGGAGG TCTGGCAGGA GGAATCCATC CAAGCTTCAC 
CACACAGAAG AGAAAGATGG ACCTCACGGT TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG 
GCCATGAAGA ACAAGAATGT AGTGAATCTG GGCGCCATCC GACAGGGTAT GAAACGCTTC 
CAGTTTCTCC TGAACTGCTG CGAGCCAGGA ACCATACCAG ATGCCTCCAT CCTCGCCGCT 
GCTCTGGACC TCGAAGCCCC AGTTGTGGCC AGGGCCTCCC TTTTCTTGGA ATGCGCCAGA 
TTTGTCCACC GGTGTAATCG TGGCAACTGG CCCGAATGGA TGAAGGGCCA CCACGTAAAC 
ATCACCAAGA GAGGGCTGTC CAGGGGCCGA TCGCCTATTG TGGGCAATAA AAGAAACCAG 
AAGCTCCAGT GGAACGCTGC CAAGCATTTC TGCCAGTGGG GAGACGCGAT TGGCACGAGG 
CTCAGCGAAC TCTGCCACTC GGACAGTGAG AGCCCCGCCA ACATCCTGGG TTATATTTAC 
GATGAAGAGA CCAAACGCAG GATGAGGAAA GAAGACGAGG AAGAAGACTA TTTAGATGAC 
AACACGGTCA ATCCTACAAA ATGTGGCTGC CCCTTCGCAC TGAAGATGGC CGCTTGCCAG 
CTGTTGTTGG AGATCACCAC TTTCCTGCGA GAGACCTTCC CCTGCTTACC ACGACCGCGC 
ACCGAACCGC TTGTGGATCT GGACAGCTGC CGCCTGCGTC TGGACCCAGA GCTCGGCCGC 
CATCGCTACG AGAGGAAGAT CAGCTTTGCG GGCATCCTCG ACGACGAAGA CGGGCACGAT 
TCCCTCAACA GCAGCAGCCA CACTCTGAAG TCTGACACCA CCTGTGATGA TAAGAAGACC 
CAGGAGGCTC AGGACAATCT CTCCCCACTC CCCTGCTCTC CAGCACCAGT GCGGAAGATT 
CGTATCGGAG GCTCGCGCCT GCTTCAGATC AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG 
GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG GGGAGTCTGA AGAGCACCAA GCTGTCTCGG 
CAGGACTCTG AGTCTGAGAA CGAAGAAGGC CTGCTGTCAC AAACGCAGAG CAGGGACACA 
GTTACTGACA TCGGCAGTCC CTTCAGCACC AGTGAACCCA GCATCGAGCC AGAAGGTCAG 
AGCTCAGGAG GGACGGAGGA CAACTACCAC CGCAACATGT CCTGGCTTCA CATTATGATC 
CTACTGTGCA ACCAGCAGAG TTTCATCTGT ACCCACATAG ACTTCTGCCA CCCACGCTGC 
TACCAACACC ACAGCCGCTC CTGCGCCCGT CTGGTCCGTG CCATCAAGCT CGTGTACGGG 
GAGACGGTGG ACAGCCTGAG GGAGGACAGC GCCTCCTCTG GTAACATCGG AGGACGTGTG 
AAGAAAAACA AAGAGTGTTC AGACAAGTCT TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA 
CCCACTGACT CCAACACAGA AGGGAAGAAG GATACAGGCA TGCTCAAGTA CATCCGCAAT 
CAGGTGATGA GTTTGTCACC AGCTCCTCTT TCACTGCTGA TCAAAGCAGC TCCTATCTTG 
ACTGACGACA TGTACGGAGA CATCCAGCCC GCAGCCTGGG AACTCCTACT CAGTGTGGAT 
GAACATATGG CAGCCGCTGC TGCTGCCTTG TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT 
GCAGTGACCG AAATGATGAT GGCAGAGTTC CAGCACCCTG AGGCCTGCCA GCGCATTAAC 
TCCATCCTGA AGTTTTACAC TCTGTGGCGT TTTCGCTACC AAGTGTGGCC TCGCATGGAG 
GAAGGAGCCC AGCAGATCTT TAAGATCCCT CCACCCAGCA TTAACTTCAC TCTCCCTTCT 
CCAATACTGG GCATGCCCTG TGTCCCCATA TTTGACCCTC CCTGGGTGCC CCAGAACACT 
GGCAGCGTCC AGGATCCAAT CAACGAAGAC CAGTCTTACC CCACATTAAT TCAGTCAGTC 
CATGCCATAA AATCCTTCTC TGCGCGGGCG GTGTCGCGCT CCCACCAGCG AGCCGAGCAC 
ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG AAGCGGCGTC TGGGCCGCGA GGCCAGCATC 
ATCACAGCCA TCCCTGTGGC TCAGGAGGCC TGCTACGAAC CCACATGCAG CCCTCCACCC 
GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG AACCTGGCAT CACGACGTCT GTCTGTCAGC 
CCATCCTGCG CCTCCAGCAA CTCCCACAGG AACTACTCTT TCCGCCGGGG CTCTGTATGG 
TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT GAAGAAAACA CAACAGAACA CACACCCACT 
CACCACATGC TACAGCCACC TCAAGCTGTC TTCCCAGCAT GCATCTGTGC TGCCGTCCTA 
CCAATAGTGC ACCTCATGGA AGACGGCGAA GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT 
GTTGCCCAGC AAATCCTCTG GAACTGCCTC ATTGAAGATC CAGCTCTCGT TCTCCGCCAC 
TTCCTGGAAA AGCTGACAGT TAGCAACAGA CAGGACGAGC TGATGTACAT GCTTAGGAAG 
CTCCTGCTCA ACATTGGAGA TCTGCCAGCC CAGACCTCTC ACATCCTTTT TAACTACCTG 
GTGGGCCTGA TCATGTATTT TGTGCGGACT CCCTGTGAAT GGGGCATGGA CGCCATCTCG 
GCCACGCTGA CCTTCCTCTG GGAGGTGGTC GGCTACGTGG AGGGGCTCTT CTTTAAAGAC 
CTGAAGCAGA CCATGAAGAA AGAGCAGTGT GAGGTCAAAC TGCTGGTCAC AGCATCGATG 
CCAGGAACCA AAACGCTGGT GGTGCACGGG CAGAATGAAT GTGACATCCC AACCCAGCTA 
CCAGTCCACG AAGACACTCA GTTTGAAGCC CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC 
ATTCCCGAGG CCAGATCGGC ACACTACTTC CTCATGGATA AACGATGGAA CCTCATCCAT 
TATTCAAAGA CATATGTTAG AGACATCTAC CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC 
AACCTGGTCC ACATGTTGCC TGAGAAAGGA CAGGAGCTCA TCCAGAAACA GGTGTTTTCC 
CGTAAACTAG AAGAAGTCGG TCGAGTCCTC TTCCTCATCT CCCTCACACA ACACATGCCA 
GCTGTGCACA AGCAGTCCCA CGTCTCCCTG CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC 
TTCCCCCGCA CCGCCATCGA TGCAGAGTTC TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG 
CTGTTTGGCT TGGACACTCT CCACAAGGTG TTGTGGATCA AACTGTTGGA GGAAATGTTT 
CTGGGCATGC CCAGTGAGTA CCCGTGGGGC GATGAGATGA TGCTGTTCCT TAACGTCTTC 
AACGGGGCCC TTCTGCTGCA CCCCGAGGAC AGCTCCCTGC TGAGGCAGTA CACGGCGACC 
GCCATCAACA CCGCCGTTCA CTTTAACCAT CTGTTCTCCC TGAGCGGGTA CCAGTGGATG 
CTGCCAACCA TGCTGCAGGT CTATGCAGAC TACGAGAGCA ACCCTCTTCT GAGGCAGGGC 
ATTGAGTTTT GCTGCCGGCA GTTTTACATC CTCCATCGAA AACCCTTTAT CCTGCAGCTG 
TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC ACAACCAGCA CCAGTACCGG TCTTTCTAAA 
GGAGTTTCGG CCCAGTGTCT GTTCGACCTG CTGGTCTCTC TAGAAGGTGA AACCCAGGAT 
GCCCTGGATG CTCTGGAGCT GGTAAAAGCT GAGAAGCCTC TACGCTCTCT GGATTTCTGT 
TATGGTAATG AGGACCTGAC CTTTTCTATC AGCGAGGCCA TCAAGCTGTG CGTCACTGTG 
GTTGCATATG CCCCCGAATC CTTCAGGAGT CTCCAGATGC TGATGGTGCT GGAGGCCTTG 
GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC AACACAGTTA CGATGGAGTC TGCTTCGGCT 
GCCAGAGATG AGATTGCGGC CATCGCTGCC CTAGCTACGT CTCTGCAGGC CCTGCTCTAC 
AGCTCTGAAA CCCTCACAAG GCCCATGACG GCTCCCCAGA TATCTCGCTG TGATCAGGGC 
CACAAGGGCG GCACTACAGC TAACCACGCC ATGTCCGGAG GGGTCAACAC CAGGGACAAT 
CTTCACCTGC TGGAGGAGGG CCAGGGCATG CCAAGGGAAG AGCTGGACGA GCGCATTGCC 
AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC CTGTTGAACA TCTGCACCGA GTTTTACAAA 
CACTGCGGGC CACGGCTCAA AATCCTACAG AACGTTGCAG GCGAGCCGCG GGTCACAGCT 
CTGGAGCTAC TGGACATCAA GTCCCACATG AGGCTGGCAG AGATCGCCCA CGCCCTGCTG 
AAGCTGGCGC CCTACGACAC CCTGACCATG GAGAGCCGAG GGCTGCGGCG TTACATCATG 
GAGATGCTGC CCATCACCGA CTGGTCGTCC GAGGCCGTCC GGCCTGCCCT CATCCTCATC 
CTCAAGAGGC TGGACCGCAT GTTCAACAAG ATCCACAAGA TGCCCACGCT CAGGTGCGCT 
CGCCCTCAGG CTTTATGCAG CAGGAGACAG GTGGAGTGGG AGGCGGCCAG TAGCCTGATC 
GAGGGGATCT GCCTGACCTT GCAGCGACAG CCGATCATCT CGTTCCTGCC TCACCTGCGC 
TCGCTTATCA ACGTCTGCGT CAACCTGGTG ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG 
GACGGGCTGC CACTCCTCCA CCTCAGCCCT TACCTGTCTC CGCCTCTGCC CTTTAGCACC 
GCCGTGGTCC GATTGGTTGC TCTGCAGATT CAGGCCTTGA AGGAAGACTT CCCCCTCAGT 
CACGTGATCT CACCTTTCAC CAATCAGGAA AGGCGGGAGG GGATGCTGCT CAACCTGCTC 
ATTCCCTTCG TGTTAACTGT AGGCTCCGGG AGCAAAGACA GCCCCCACCT GGAGCAGCCG 
GAGGTCTTCC TGCTCCTACA GACAGTCATC AACATCCTGC TTCCCCCTCG TATCATCTCC 
ACATCCCGCA CCAAGAACTT TATGCTTGAC GCCTCTCCGG CTCACTGCTC CACGCCGGGT 
GACCCTGGGA AAGATCTGCG TAGGGAGGGA TTAGCAGAGT CCACGAGCCA GGCTGCTTAT 
TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC GAGCGCTCGC TGGGGAACCA GTGGTATCGG 
CTGAGCCTGC AGGTGAAGGA GATGGCTCTG AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC 
TTCATCGACT TTATCGTCCG AACCCGGATC CCTATTTTTG TCCTGCTCAG ACCCTTTATA 
CAGTGCAAGT TGCTGACCCA GCCGGCCGAT TCCCAGGAAG AGATCACAGC GCGCCACCAC 
ATCGCTGATC AGCTGGAGCG GCGCTTCATC CCACGACCTC TCTGCAAGAG CTCACTGTTT 
GCAGAGTTCA ACAATGAGCT CAAGATCCTC AAAGAGGCTG TGCACAGCGG CTCAGCATAT 
CAGGGCAAGA CATCCATCAG CACGGTGGGC ACCTCCACGT CAGCCTACCG CCTCAGTTTG 
GCCACAATGT CGCGATCCAA CACAGGCACT GGAACAGTTT GGGAGCAGGA CAGCCAACCG 
TCACGGCAGC CCTCACAGGA CACGCTCAGC CGTACCGACG AGGACGATGA AGAGATTGAC 
TCCATGAGCA TTCCCAGCGT GGTGAGCGAG CACGAAGCCT TCATGCCCAG GGTGATGTCA 
CAGCGCCGTT TCTCCAGCCA CGCCACTGGC TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC 
ACCACCATGT TGCCCAGCCA CAGTGAACCC AATGTGCTAG ATGAGTCCCA GGGTCTTCTG 
CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT GTGCAGAGCG AACCAGGTCA GAACCTCCTG 
ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA CTCAGACAGA TGCGACGCCC CCTGCTGTCC 
ATTCCAAAAA CCGAACCACG CGGTCGATCT GGAGCGAGGC TTTCTACAAC GCGCAGGAGC 
ATCCAGCCCA AGAACAAACC ACTGGAAACT TTATTGGACT GTGAAGCGCA TGGAGACCAA 
AAGAGGTCAG TCACCTTTAC AGAGAATCAA GGCCAGCAGG CATCCACGGG GGCAAGCAAG 
CCTCCAACTC CCAGCACTGT GGACCCACCG GGCGAAGGCA GAGGGACGAG TCCTGCCCCA 
TCAAAGTCCC CAACCCTGGA AGTACCCCCC ACCTCTTCAG CCATGATCTT GGCTGACCTG 
CACGGACCTA CAAATGCACA GGTCCCTCAA GCATTAAGCA GCAAGGAGAA GAGAGAGCAG 
TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC CCATCCATCT CCCGGCCTTC CACTCCAACA 
CCCCCATCCC GAACCTGCTC CCCTCTCCCC CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG 
TCCCGCACCT CCTCTCCTCT TCCACCGCCA CTCCCAGTGC TGAGCACAGC ACCCGCTCCG 
GGTCCACCGC CGCCACCTCC ATTACCACCA ACTCATCTTG CTGCTGCTCC TCCTCCTCCA 
CCACCCCCGG GGCCTTCTAG GATCAGAGAG AGCCCCGGGG ACGAGGACAC AACCGCCCTG 
CTGCCGCGCA GCGACACCCT GCTGCACCTC AGCGAGGACG ACGGCACCGA GAACCCGCTT 
CTCACCCCGC TGCTGTCCCC TCCTCCCCCC CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC 
CTCTCACCCG TCGACCTGGA CCTGGATGAG TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_023153470.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009238.1 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.

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CloneID	OMa313320
Clone ID Related Accession (Same CDS sequence)	XM_023153470.1
Accession Version	XM_023153470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGT 
GAAGAACAGA TCCCGGGTGC TCTGCTGGGC AGGAAGGATT TTTGGAGGAA GATGTTCAAG 
TCGCAGAGCG CCGCCAGTGA CACCAGCAGC CAGTCGGAAC AAGACACCTC AGAGTGCACC 
ACCGCACACT CTGGCACCAC CACCGACCGC CGCTCACGAT CCAGATCCCG TCGCATTTCG 
CTTCGCAAGA AGTTGAAGCT GCCTATCGGG AACTGGTTGA AGCGCTCCTC TCTCTCTGGA 
CTGACTGACG GCGTCGAGGA CTTGCTGGAT ATCAGCTCGG TGGATCGACT CTCCTTCATA 
CGTCAGAGTT CAAAGGTGAA GTTCACTAGT GCAGTGAAGC TATCTGAAGG TGGCGCTGTA 
GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG AATTTCTTCA AGCGGCTCGG TAAATGGAGG 
TCTGGCAGGA GGAATCCATC CAAGCTTCAC CACACAGAAG AGAAAGATGG ACCTCACGGT 
TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG GCCATGAAGA ACAAGAATGT AGTGAATCTG 
GGCGCCATCC GACAGGGTAT GAAACGCTTC CAGTTTCTCC TGAACTGCTG CGAGCCAGGA 
ACCATACCAG ATGCCTCCAT CCTCGCCGCT GCTCTGGACC TCGAAGCCCC AGTTGTGGCC 
AGGGCCTCCC TTTTCTTGGA ATGCGCCAGA TTTGTCCACC GGTGTAATCG TGGCAACTGG 
CCCGAATGGA TGAAGGGCCA CCACGTAAAC ATCACCAAGA GAGGGCTGTC CAGGGGCCGA 
TCGCCTATTG TGGGCAATAA AAGAAACCAG AAGCTCCAGT GGAACGCTGC CAAGCATTTC 
TGCCAGTGGG GAGACGCGAT TGGCACGAGG CTCAGCGAAC TCTGCCACTC GGACAGTGAG 
AGCCCCGCCA ACATCCTGGG TTATATTTAC GATGAAGAGA CCAAACGCAG GATGAGGAAA 
GAAGACGAGG AAGAAGACTA TTTAGATGAC AACACGGTCA ATCCTACAAA ATGTGGCTGC 
CCCTTCGCAC TGAAGATGGC CGCTTGCCAG CTGTTGTTGG AGATCACCAC TTTCCTGCGA 
GAGACCTTCC CCTGCTTACC ACGACCGCGC ACCGAACCGC TTGTGGATCT GGACAGCTGC 
CGCCTGCGTC TGGACCCAGA GCTCGGCCGC CATCGCTACG AGAGGAAGAT CAGCTTTGCG 
GGCATCCTCG ACGACGAAGA CGGGCACGAT TCCCTCAACA GCAGCAGCCA CACTCTGAAG 
TCTGACACCA CCTGTGATGA TAAGAAGACC CAGGAGGCTC AGGACAATCT CTCCCCACTC 
CCCTGCTCTC CAGCACCAGT GCGGAAGATT CGTATCGGAG GCTCGCGCCT GCTTCAGATC 
AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG 
GGGAGTCTGA AGAGCACCAA GCTGTCTCGG CAGGACTCTG AGTCTGAGAA CGAAGAAGGC 
CTGCTGTCAC AAACGCAGAG CAGGGACACA GTTACTGACA TCGGCAGTCC CTTCAGCACC 
AGTGAACCCA GCATCGAGCC AGAAGGTCAG AGCTCAGGAG GGACGGAGGA CAACTACCAC 
CGCAACATGT CCTGGCTTCA CATTATGATC CTACTGTGCA ACCAGCAGAG TTTCATCTGT 
ACCCACATAG ACTTCTGCCA CCCACGCTGC TACCAACACC ACAGCCGCTC CTGCGCCCGT 
CTGGTCCGTG CCATCAAGCT CGTGTACGGG GAGACGGTGG ACAGCCTGAG GGAGGACAGC 
GCCTCCTCTG GTAACATCGG AGGACGTGTG AAGAAAAACA AAGAGTGTTC AGACAAGTCT 
TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA CCCACTGACT CCAACACAGA AGGGAAGAAG 
GATACAGGCA TGCTCAAGTA CATCCGCAAT CAGGTGATGA GTTTGTCACC AGCTCCTCTT 
TCACTGCTGA TCAAAGCAGC TCCTATCTTG ACTGACGACA TGTACGGAGA CATCCAGCCC 
GCAGCCTGGG AACTCCTACT CAGTGTGGAT GAACATATGG CAGCCGCTGC TGCTGCCTTG 
TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT GCAGTGACCG AAATGATGAT GGCAGAGTTC 
CAGCACCCTG AGGCCTGCCA GCGCATTAAC TCCATCCTGA AGTTTTACAC TCTGTGGCGT 
TTTCGCTACC AAGTGTGGCC TCGCATGGAG GAAGGAGCCC AGCAGATCTT TAAGATCCCT 
CCACCCAGCA TTAACTTCAC TCTCCCTTCT CCAATACTGG GCATGCCCTG TGTCCCCATA 
TTTGACCCTC CCTGGGTGCC CCAGAACACT GGCAGCGTCC AGGATCCAAT CAACGAAGAC 
CAGTCTTACC CCACATTAAT TCAGTCAGTC CATGCCATAA AATCCTTCTC TGCGCGGGCG 
GTGTCGCGCT CCCACCAGCG AGCCGAGCAC ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG 
AAGCGGCGTC TGGGCCGCGA GGCCAGCATC ATCACAGCCA TCCCTGTGGC TCAGGAGGCC 
TGCTACGAAC CCACATGCAG CCCTCCACCC GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG 
AACCTGGCAT CACGACGTCT GTCTGTCAGC CCATCCTGCG CCTCCAGCAA CTCCCACAGG 
AACTACTCTT TCCGCCGGGG CTCTGTATGG TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT 
GAAGAAAACA CAACAGAACA CACACCCACT CACCACATGC TACAGCCACC TCAAGCTGTC 
TTCCCAGCAT GCATCTGTGC TGCCGTCCTA CCAATAGTGC ACCTCATGGA AGACGGCGAA 
GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT GTTGCCCAGC AAATCCTCTG GAACTGCCTC 
ATTGAAGATC CAGCTCTCGT TCTCCGCCAC TTCCTGGAAA AGCTGACAGT TAGCAACAGA 
CAGGACGAGC TGATGTACAT GCTTAGGAAG CTCCTGCTCA ACATTGGAGA TCTGCCAGCC 
CAGACCTCTC ACATCCTTTT TAACTACCTG GTGGGCCTGA TCATGTATTT TGTGCGGACT 
CCCTGTGAAT GGGGCATGGA CGCCATCTCG GCCACGCTGA CCTTCCTCTG GGAGGTGGTC 
GGCTACGTGG AGGGGCTCTT CTTTAAAGAC CTGAAGCAGA CCATGAAGAA AGAGCAGTGT 
GAGGTCAAAC TGCTGGTCAC AGCATCGATG CCAGGAACCA AAACGCTGGT GGTGCACGGG 
CAGAATGAAT GTGACATCCC AACCCAGCTA CCAGTCCACG AAGACACTCA GTTTGAAGCC 
CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC ATTCCCGAGG CCAGATCGGC ACACTACTTC 
CTCATGGATA AACGATGGAA CCTCATCCAT TATTCAAAGA CATATGTTAG AGACATCTAC 
CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC AACCTGGTCC ACATGTTGCC TGAGAAAGGA 
CAGGAGCTCA TCCAGAAACA GGTGTTTTCC CGTAAACTAG AAGAAGTCGG TCGAGTCCTC 
TTCCTCATCT CCCTCACACA ACACATGCCA GCTGTGCACA AGCAGTCCCA CGTCTCCCTG 
CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC TTCCCCCGCA CCGCCATCGA TGCAGAGTTC 
TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG CTGTTTGGCT TGGACACTCT CCACAAGGTG 
TTGTGGATCA AACTGTTGGA GGAAATGTTT CTGGGCATGC CCAGTGAGTA CCCGTGGGGC 
GATGAGATGA TGCTGTTCCT TAACGTCTTC AACGGGGCCC TTCTGCTGCA CCCCGAGGAC 
AGCTCCCTGC TGAGGCAGTA CACGGCGACC GCCATCAACA CCGCCGTTCA CTTTAACCAT 
CTGTTCTCCC TGAGCGGGTA CCAGTGGATG CTGCCAACCA TGCTGCAGGT CTATGCAGAC 
TACGAGAGCA ACCCTCTTCT GAGGCAGGGC ATTGAGTTTT GCTGCCGGCA GTTTTACATC 
CTCCATCGAA AACCCTTTAT CCTGCAGCTG TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC 
ACAACCAGCA CCAGTACCGG TCTTTCTAAA GGAGTTTCGG CCCAGTGTCT GTTCGACCTG 
CTGGTCTCTC TAGAAGGTGA AACCCAGGAT GCCCTGGATG CTCTGGAGCT GGTAAAAGCT 
GAGAAGCCTC TACGCTCTCT GGATTTCTGT TATGGTAATG AGGACCTGAC CTTTTCTATC 
AGCGAGGCCA TCAAGCTGTG CGTCACTGTG GTTGCATATG CCCCCGAATC CTTCAGGAGT 
CTCCAGATGC TGATGGTGCT GGAGGCCTTG GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC 
AACACAGTTA CGATGGAGTC TGCTTCGGCT GCCAGAGATG AGATTGCGGC CATCGCTGCC 
CTAGCTACGT CTCTGCAGGC CCTGCTCTAC AGCTCTGAAA CCCTCACAAG GCCCATGACG 
GCTCCCCAGA TATCTCGCTG TGATCAGGGC CACAAGGGCG GCACTACAGC TAACCACGCC 
ATGTCCGGAG GGGTCAACAC CAGGGACAAT CTTCACCTGC TGGAGGAGGG CCAGGGCATG 
CCAAGGGAAG AGCTGGACGA GCGCATTGCC AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC 
CTGTTGAACA TCTGCACCGA GTTTTACAAA CACTGCGGGC CACGGCTCAA AATCCTACAG 
AACGTTGCAG GCGAGCCGCG GGTCACAGCT CTGGAGCTAC TGGACATCAA GTCCCACATG 
AGGCTGGCAG AGATCGCCCA CGCCCTGCTG AAGCTGGCGC CCTACGACAC CCTGACCATG 
GAGAGCCGAG GGCTGCGGCG TTACATCATG GAGATGCTGC CCATCACCGA CTGGTCGTCC 
GAGGCCGTCC GGCCTGCCCT CATCCTCATC CTCAAGAGGC TGGACCGCAT GTTCAACAAG 
ATCCACAAGA TGCCCACGCT CAGGTGCGCT CGCCCTCAGG CTTTATGCAG CAGGAGACAG 
GTGGAGTGGG AGGCGGCCAG TAGCCTGATC GAGGGGATCT GCCTGACCTT GCAGCGACAG 
CCGATCATCT CGTTCCTGCC TCACCTGCGC TCGCTTATCA ACGTCTGCGT CAACCTGGTG 
ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG GACGGGCTGC CACTCCTCCA CCTCAGCCCT 
TACCTGTCTC CGCCTCTGCC CTTTAGCACC GCCGTGGTCC GATTGGTTGC TCTGCAGATT 
CAGGCCTTGA AGGAAGACTT CCCCCTCAGT CACGTGATCT CACCTTTCAC CAATCAGGAA 
AGGCGGGAGG GGATGCTGCT CAACCTGCTC ATTCCCTTCG TGTTAACTGT AGGCTCCGGG 
AGCAAAGACA GCCCCCACCT GGAGCAGCCG GAGGTCTTCC TGCTCCTACA GACAGTCATC 
AACATCCTGC TTCCCCCTCG TATCATCTCC ACATCCCGCA CCAAGAACTT TATGCTTGAC 
GCCTCTCCGG CTCACTGCTC CACGCCGGGT GACCCTGGGA AAGATCTGCG TAGGGAGGGA 
TTAGCAGAGT CCACGAGCCA GGCTGCTTAT TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC 
GAGCGCTCGC TGGGGAACCA GTGGTATCGG CTGAGCCTGC AGGTGAAGGA GATGGCTCTG 
AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC TTCATCGACT TTATCGTCCG AACCCGGATC 
CCTATTTTTG TCCTGCTCAG ACCCTTTATA CAGTGCAAGT TGCTGACCCA GCCGGCCGAT 
TCCCAGGAAG AGATCACAGC GCGCCACCAC ATCGCTGATC AGCTGGAGCG GCGCTTCATC 
CCACGACCTC TCTGCAAGAG CTCACTGTTT GCAGAGTTCA ACAATGAGCT CAAGATCCTC 
AAAGAGGCTG TGCACAGCGG CTCAGCATAT CAGGGCAAGA CATCCATCAG CACGGTGGGC 
ACCTCCACGT CAGCCTACCG CCTCAGTTTG GCCACAATGT CGCGATCCAA CACAGGCACT 
GGAACAGTTT GGGAGCAGGA CAGCCAACCG TCACGGCAGC CCTCACAGGA CACGCTCAGC 
CGTACCGACG AGGACGATGA AGAGATTGAC TCCATGAGCA TTCCCAGCGT GGTGAGCGAG 
CACGAAGCCT TCATGCCCAG GGTGATGTCA CAGCGCCGTT TCTCCAGCCA CGCCACTGGC 
TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC ACCACCATGT TGCCCAGCCA CAGTGAACCC 
AATGTGCTAG ATGAGTCCCA GGGTCTTCTG CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT 
GTGCAGAGCG AACCAGGTCA GAACCTCCTG ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA 
CTCAGACAGA TGCGACGCCC CCTGCTGTCC ATTCCAAAAA CCGAACCACG CGGTCGATCT 
GGAGCGAGGC TTTCTACAAC GCGCAGGAGC ATCCAGCCCA AGAACAAACC ACTGGAAACT 
TTATTGGACT GTGAAGCGCA TGGAGACCAA AAGAGGTCAG TCACCTTTAC AGAGAATCAA 
GGCCAGCAGG CATCCACGGG GGCAAGCAAG CCTCCAACTC CCAGCACTGT GGACCCACCG 
GGCGAAGGCA GAGGGACGAG TCCTGCCCCA TCAAAGTCCC CAACCCTGGA AGTACCCCCC 
ACCTCTTCAG CCATGATCTT GGCTGACCTG CACGGACCTA CAAATGCACA GGTCCCTCAA 
GCATTAAGCA GCAAGGAGAA GAGAGAGCAG TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC 
CCATCCATCT CCCGGCCTTC CACTCCAACA CCCCCATCCC GAACCTGCTC CCCTCTCCCC 
CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG TCCCGCACCT CCTCTCCTCT TCCACCGCCA 
CTCCCAGTGC TGAGCACAGC ACCCGCTCCG GGTCCACCGC CGCCACCTCC ATTACCACCA 
ACTCATCTTG CTGCTGCTCC TCCTCCTCCA CCACCCCCGG GGCCTTCTAG GATCAGAGAG 
AGCCCCGGGG ACGAGGACAC AACCGCCCTG CTGCCGCGCA GCGACACCCT GCTGCACCTC 
AGCGAGGACG ACGGCACCGA GAACCCGCTT CTCACCCCGC TGCTGTCCCC TCCTCCCCCC 
CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC CTCTCACCCG TCGACCTGGA CCTGGATGAG 
TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009238.1
CDS	399..10790
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEARVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRSEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_023153470.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153470.1

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAAGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATCCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAAAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGCTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGT 
GAAGAACAGA TCCCGGGTGC TCTGCTGGGC AGGAAGGATT TTTGGAGGAA GATGTTCAAG 
TCGCAGAGCG CCGCCAGTGA CACCAGCAGC CAGTCGGAAC AAGACACCTC AGAGTGCACC 
ACCGCACACT CTGGCACCAC CACCGACCGC CGCTCACGAT CCAGATCCCG TCGCATTTCG 
CTTCGCAAGA AGTTGAAGCT GCCTATCGGG AACTGGTTGA AGCGCTCCTC TCTCTCTGGA 
CTGACTGACG GCGTCGAGGA CTTGCTGGAT ATCAGCTCGG TGGATCGACT CTCCTTCATA 
CGTCAGAGTT CAAAGGTGAA GTTCACTAGT GCAGTGAAGC TATCTGAAGG TGGCGCTGTA 
GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG AATTTCTTCA AGCGGCTCGG TAAATGGAGG 
TCTGGCAGGA GGAATCCATC CAAGCTTCAC CACACAGAAG AGAAAGATGG ACCTCACGGT 
TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG GCCATGAAGA ACAAGAATGT AGTGAATCTG 
GGCGCCATCC GACAGGGTAT GAAACGCTTC CAGTTTCTCC TGAACTGCTG CGAGCCAGGA 
ACCATACCAG ATGCCTCCAT CCTCGCCGCT GCTCTGGACC TCGAAGCCCC AGTTGTGGCC 
AGGGCCTCCC TTTTCTTGGA ATGCGCCAGA TTTGTCCACC GGTGTAATCG TGGCAACTGG 
CCCGAATGGA TGAAGGGCCA CCACGTAAAC ATCACCAAGA GAGGGCTGTC CAGGGGCCGA 
TCGCCTATTG TGGGCAATAA AAGAAACCAG AAGCTCCAGT GGAACGCTGC CAAGCATTTC 
TGCCAGTGGG GAGACGCGAT TGGCACGAGG CTCAGCGAAC TCTGCCACTC GGACAGTGAG 
AGCCCCGCCA ACATCCTGGG TTATATTTAC GATGAAGAGA CCAAACGCAG GATGAGGAAA 
GAAGACGAGG AAGAAGACTA TTTAGATGAC AACACGGTCA ATCCTACAAA ATGTGGCTGC 
CCCTTCGCAC TGAAGATGGC CGCTTGCCAG CTGTTGTTGG AGATCACCAC TTTCCTGCGA 
GAGACCTTCC CCTGCTTACC ACGACCGCGC ACCGAACCGC TTGTGGATCT GGACAGCTGC 
CGCCTGCGTC TGGACCCAGA GCTCGGCCGC CATCGCTACG AGAGGAAGAT CAGCTTTGCG 
GGCATCCTCG ACGACGAAGA CGGGCACGAT TCCCTCAACA GCAGCAGCCA CACTCTGAAG 
TCTGACACCA CCTGTGATGA TAAGAAGACC CAGGAGGCTC AGGACAATCT CTCCCCACTC 
CCCTGCTCTC CAGCACCAGT GCGGAAGATT CGTATCGGAG GCTCGCGCCT GCTTCAGATC 
AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG 
GGGAGTCTGA AGAGCACCAA GCTGTCTCGG CAGGACTCTG AGTCTGAGAA CGAAGAAGGC 
CTGCTGTCAC AAACGCAGAG CAGGGACACA GTTACTGACA TCGGCAGTCC CTTCAGCACC 
AGTGAACCCA GCATCGAGCC AGAAGGTCAG AGCTCAGGAG GGACGGAGGA CAACTACCAC 
CGCAACATGT CCTGGCTTCA CATTATGATC CTACTGTGCA ACCAGCAGAG TTTCATCTGT 
ACCCACATAG ACTTCTGCCA CCCACGCTGC TACCAACACC ACAGCCGCTC CTGCGCCCGT 
CTGGTCCGTG CCATCAAGCT CGTGTACGGG GAGACGGTGG ACAGCCTGAG GGAGGACAGC 
GCCTCCTCTG GTAACATCGG AGGACGTGTG AAGAAAAACA AAGAGTGTTC AGACAAGTCT 
TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA CCCACTGACT CCAACACAGA AGGGAAGAAG 
GATACAGGCA TGCTCAAGTA CATCCGCAAT CAGGTGATGA GTTTGTCACC AGCTCCTCTT 
TCACTGCTGA TCAAAGCAGC TCCTATCTTG ACTGACGACA TGTACGGAGA CATCCAGCCC 
GCAGCCTGGG AACTCCTACT CAGTGTGGAT GAACATATGG CAGCCGCTGC TGCTGCCTTG 
TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT GCAGTGACCG AAATGATGAT GGCAGAGTTC 
CAGCACCCTG AGGCCTGCCA GCGCATTAAC TCCATCCTGA AGTTTTACAC TCTGTGGCGT 
TTTCGCTACC AAGTGTGGCC TCGCATGGAG GAAGGAGCCC AGCAGATCTT TAAGATCCCT 
CCACCCAGCA TTAACTTCAC TCTCCCTTCT CCAATACTGG GCATGCCCTG TGTCCCCATA 
TTTGACCCTC CCTGGGTGCC CCAGAACACT GGCAGCGTCC AGGATCCAAT CAACGAAGAC 
CAGTCTTACC CCACATTAAT TCAGTCAGTC CATGCCATAA AATCCTTCTC TGCGCGGGCG 
GTGTCGCGCT CCCACCAGCG AGCCGAGCAC ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG 
AAGCGGCGTC TGGGCCGCGA GGCCAGCATC ATCACAGCCA TCCCTGTGGC TCAGGAGGCC 
TGCTACGAAC CCACATGCAG CCCTCCACCC GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG 
AACCTGGCAT CACGACGTCT GTCTGTCAGC CCATCCTGCG CCTCCAGCAA CTCCCACAGG 
AACTACTCTT TCCGCCGGGG CTCTGTATGG TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT 
GAAGAAAACA CAACAGAACA CACACCCACT CACCACATGC TACAGCCACC TCAAGCTGTC 
TTCCCAGCAT GCATCTGTGC TGCCGTCCTA CCAATAGTGC ACCTCATGGA AGACGGCGAA 
GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT GTTGCCCAGC AAATCCTCTG GAACTGCCTC 
ATTGAAGATC CAGCTCTCGT TCTCCGCCAC TTCCTGGAAA AGCTGACAGT TAGCAACAGA 
CAGGACGAGC TGATGTACAT GCTTAGGAAG CTCCTGCTCA ACATTGGAGA TCTGCCAGCC 
CAGACCTCTC ACATCCTTTT TAACTACCTG GTGGGCCTGA TCATGTATTT TGTGCGGACT 
CCCTGTGAAT GGGGCATGGA CGCCATCTCG GCCACGCTGA CCTTCCTCTG GGAGGTGGTC 
GGCTACGTGG AGGGGCTCTT CTTTAAAGAC CTGAAGCAGA CCATGAAGAA AGAGCAGTGT 
GAGGTCAAAC TGCTGGTCAC AGCATCGATG CCAGGAACCA AAACGCTGGT GGTGCACGGG 
CAGAATGAAT GTGACATCCC AACCCAGCTA CCAGTCCACG AAGACACTCA GTTTGAAGCC 
CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC ATTCCCGAGG CCAGATCGGC ACACTACTTC 
CTCATGGATA AACGATGGAA CCTCATCCAT TATTCAAAGA CATATGTTAG AGACATCTAC 
CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC AACCTGGTCC ACATGTTGCC TGAGAAAGGA 
CAGGAGCTCA TCCAGAAACA GGTGTTTTCC CGTAAACTAG AAGAAGTCGG TCGAGTCCTC 
TTCCTCATCT CCCTCACACA ACACATGCCA GCTGTGCACA AGCAGTCCCA CGTCTCCCTG 
CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC TTCCCCCGCA CCGCCATCGA TGCAGAGTTC 
TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG CTGTTTGGCT TGGACACTCT CCACAAGGTG 
TTGTGGATCA AACTGTTGGA GGAAATGTTT CTGGGCATGC CCAGTGAGTA CCCGTGGGGC 
GATGAGATGA TGCTGTTCCT TAACGTCTTC AACGGGGCCC TTCTGCTGCA CCCCGAGGAC 
AGCTCCCTGC TGAGGCAGTA CACGGCGACC GCCATCAACA CCGCCGTTCA CTTTAACCAT 
CTGTTCTCCC TGAGCGGGTA CCAGTGGATG CTGCCAACCA TGCTGCAGGT CTATGCAGAC 
TACGAGAGCA ACCCTCTTCT GAGGCAGGGC ATTGAGTTTT GCTGCCGGCA GTTTTACATC 
CTCCATCGAA AACCCTTTAT CCTGCAGCTG TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC 
ACAACCAGCA CCAGTACCGG TCTTTCTAAA GGAGTTTCGG CCCAGTGTCT GTTCGACCTG 
CTGGTCTCTC TAGAAGGTGA AACCCAGGAT GCCCTGGATG CTCTGGAGCT GGTAAAAGCT 
GAGAAGCCTC TACGCTCTCT GGATTTCTGT TATGGTAATG AGGACCTGAC CTTTTCTATC 
AGCGAGGCCA TCAAGCTGTG CGTCACTGTG GTTGCATATG CCCCCGAATC CTTCAGGAGT 
CTCCAGATGC TGATGGTGCT GGAGGCCTTG GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC 
AACACAGTTA CGATGGAGTC TGCTTCGGCT GCCAGAGATG AGATTGCGGC CATCGCTGCC 
CTAGCTACGT CTCTGCAGGC CCTGCTCTAC AGCTCTGAAA CCCTCACAAG GCCCATGACG 
GCTCCCCAGA TATCTCGCTG TGATCAGGGC CACAAGGGCG GCACTACAGC TAACCACGCC 
ATGTCCGGAG GGGTCAACAC CAGGGACAAT CTTCACCTGC TGGAGGAGGG CCAGGGCATG 
CCAAGGGAAG AGCTGGACGA GCGCATTGCC AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC 
CTGTTGAACA TCTGCACCGA GTTTTACAAA CACTGCGGGC CACGGCTCAA AATCCTACAG 
AACGTTGCAG GCGAGCCGCG GGTCACAGCT CTGGAGCTAC TGGACATCAA GTCCCACATG 
AGGCTGGCAG AGATCGCCCA CGCCCTGCTG AAGCTGGCGC CCTACGACAC CCTGACCATG 
GAGAGCCGAG GGCTGCGGCG TTACATCATG GAGATGCTGC CCATCACCGA CTGGTCGTCC 
GAGGCCGTCC GGCCTGCCCT CATCCTCATC CTCAAGAGGC TGGACCGCAT GTTCAACAAG 
ATCCACAAGA TGCCCACGCT CAGGTGCGCT CGCCCTCAGG CTTTATGCAG CAGGAGACAG 
GTGGAGTGGG AGGCGGCCAG TAGCCTGATC GAGGGGATCT GCCTGACCTT GCAGCGACAG 
CCGATCATCT CGTTCCTGCC TCACCTGCGC TCGCTTATCA ACGTCTGCGT CAACCTGGTG 
ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG GACGGGCTGC CACTCCTCCA CCTCAGCCCT 
TACCTGTCTC CGCCTCTGCC CTTTAGCACC GCCGTGGTCC GATTGGTTGC TCTGCAGATT 
CAGGCCTTGA AGGAAGACTT CCCCCTCAGT CACGTGATCT CACCTTTCAC CAATCAGGAA 
AGGCGGGAGG GGATGCTGCT CAACCTGCTC ATTCCCTTCG TGTTAACTGT AGGCTCCGGG 
AGCAAAGACA GCCCCCACCT GGAGCAGCCG GAGGTCTTCC TGCTCCTACA GACAGTCATC 
AACATCCTGC TTCCCCCTCG TATCATCTCC ACATCCCGCA CCAAGAACTT TATGCTTGAC 
GCCTCTCCGG CTCACTGCTC CACGCCGGGT GACCCTGGGA AAGATCTGCG TAGGGAGGGA 
TTAGCAGAGT CCACGAGCCA GGCTGCTTAT TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC 
GAGCGCTCGC TGGGGAACCA GTGGTATCGG CTGAGCCTGC AGGTGAAGGA GATGGCTCTG 
AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC TTCATCGACT TTATCGTCCG AACCCGGATC 
CCTATTTTTG TCCTGCTCAG ACCCTTTATA CAGTGCAAGT TGCTGACCCA GCCGGCCGAT 
TCCCAGGAAG AGATCACAGC GCGCCACCAC ATCGCTGATC AGCTGGAGCG GCGCTTCATC 
CCACGACCTC TCTGCAAGAG CTCACTGTTT GCAGAGTTCA ACAATGAGCT CAAGATCCTC 
AAAGAGGCTG TGCACAGCGG CTCAGCATAT CAGGGCAAGA CATCCATCAG CACGGTGGGC 
ACCTCCACGT CAGCCTACCG CCTCAGTTTG GCCACAATGT CGCGATCCAA CACAGGCACT 
GGAACAGTTT GGGAGCAGGA CAGCCAACCG TCACGGCAGC CCTCACAGGA CACGCTCAGC 
CGTACCGACG AGGACGATGA AGAGATTGAC TCCATGAGCA TTCCCAGCGT GGTGAGCGAG 
CACGAAGCCT TCATGCCCAG GGTGATGTCA CAGCGCCGTT TCTCCAGCCA CGCCACTGGC 
TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC ACCACCATGT TGCCCAGCCA CAGTGAACCC 
AATGTGCTAG ATGAGTCCCA GGGTCTTCTG CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT 
GTGCAGAGCG AACCAGGTCA GAACCTCCTG ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA 
CTCAGACAGA TGCGACGCCC CCTGCTGTCC ATTCCAAAAA CCGAACCACG CGGTCGATCT 
GGAGCGAGGC TTTCTACAAC GCGCAGGAGC ATCCAGCCCA AGAACAAACC ACTGGAAACT 
TTATTGGACT GTGAAGCGCA TGGAGACCAA AAGAGGTCAG TCACCTTTAC AGAGAATCAA 
GGCCAGCAGG CATCCACGGG GGCAAGCAAG CCTCCAACTC CCAGCACTGT GGACCCACCG 
GGCGAAGGCA GAGGGACGAG TCCTGCCCCA TCAAAGTCCC CAACCCTGGA AGTACCCCCC 
ACCTCTTCAG CCATGATCTT GGCTGACCTG CACGGACCTA CAAATGCACA GGTCCCTCAA 
GCATTAAGCA GCAAGGAGAA GAGAGAGCAG TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC 
CCATCCATCT CCCGGCCTTC CACTCCAACA CCCCCATCCC GAACCTGCTC CCCTCTCCCC 
CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG TCCCGCACCT CCTCTCCTCT TCCACCGCCA 
CTCCCAGTGC TGAGCACAGC ACCCGCTCCG GGTCCACCGC CGCCACCTCC ATTACCACCA 
ACTCATCTTG CTGCTGCTCC TCCTCCTCCA CCACCCCCGG GGCCTTCTAG GATCAGAGAG 
AGCCCCGGGG ACGAGGACAC AACCGCCCTG CTGCCGCGCA GCGACACCCT GCTGCACCTC 
AGCGAGGACG ACGGCACCGA GAACCCGCTT CTCACCCCGC TGCTGTCCCC TCCTCCCCCC 
CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC CTCTCACCCG TCGACCTGGA CCTGGATGAG 
TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562574.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562631.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.

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CloneID	OMa370045
Clone ID Related Accession (Same CDS sequence)	XM_004562574.5
Accession Version	XM_004562574.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562574.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562631.2
CDS	398..10888
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562574.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562574.5

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562575.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562632.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.

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CloneID	OMa370046
Clone ID Related Accession (Same CDS sequence)	XM_004562575.5
Accession Version	XM_004562575.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562575.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGTGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTGCAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGATGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562632.2
CDS	397..10884
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562575.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562575.5

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGAAGAA GTGGTGAACC TGGCATCACG ACGTCTGTCT 
GTCAGCCCAT CCTGCGCCTC CAGCAACTCC CACAGGAACT ACTCTTTCCG CCGGGGCTCT 
GTATGGTCTG TCCGCTCGCT GGCCAGTGCT GAAGATGAAG AAAACACAAC AGAACACACA 
CCCACTCACC ACATGCTACA GCCACCTCAA GCTGTCTTCC CAGCATGCAT CTGTGCTGCC 
GTCCTACCAA TAGTGCACCT CATGGAAGAC GGTGAAGTTA GAGAGGATGG CGTGGCTGTG 
AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC TGCCTCATTG AAGATCCAGC TCTCGTTCTC 
CGCCACTTCC TGGAAAAGCT GACAGTTAGC AACAGACAGG ACGAGCTGAT GTACATGCTT 
AGGAAGCTCC TGCTCAACAT TGGAGATCTG CCAGCCCAGA CCTCTCACAT CCTTTTTAAC 
TACCTGGTGG GCCTGATCAT GTATTTTGTG CGGACTCCCT GTGAATGGGG CATGGACGCC 
ATCTCGGCCA CGCTGACCTT CCTCTGGGAG GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT 
AAAGACCTGA AGCAGACCAT GAAGAAAGAG CAGTGTGAGG TCAAACTGCT GGTCACAGCA 
TCGATGCCAG GAACCAAAAC GCTGGTGGTG CACGGGCAGA ATGAATGTGA CATCCCAACC 
CAGCTACCAG TCCACGAAGA CACTCAGTTT GAAGCCCTGC TAAAGGAGTG TCTGGAATTT 
TTCAACATTC CCGAGGCCAG ATCGGCACAC TACTTCCTCA TGGATAAACG ATGGAACCTC 
ATCCATTATT CAAAGACATA TGTTAGAGAC ATCTACCCGT TCCGAAGGTC AGTCTCTCCT 
CAGCTCAACC TGGTCCACAT GTTGCCTGAG AAAGGACAGG AGCTCATCCA GAAACAGGTG 
TTTTCCCGTA AACTAGAAGA AGTCGGTCGA GTCCTCTTCC TCATCTCCCT CACACAACAC 
ATGCCAGCTG TGCACAAGCA GTCCCACGTC TCCCTGCTGC AGGAGGACCT GCTTCGCCTG 
CCCTCCTTCC CCCGCACCGC CATCGATGCA GAGTTCTCCC TCTTTAATGA GCCTCAAGGA 
AAAGAGCTGT TTGGCTTGGA CACTCTCCAC AAGGTGTTGT GGATCAAACT GTTGGAGGAA 
ATGTTTCTGG GCATGCCCAG TGAGTACCCG TGGGGCGATG AGATGATGCT GTTCCTTAAC 
GTCTTCAACG GGGCCCTTCT GCTGCACCCC GAGGACAGCT CCCTGCTGAG GCAGTACACG 
GCGACCGCCA TCAACACCGC CGTTCACTTT AACCATCTGT TCTCCCTGAG CGGGTACCAG 
TGGATGCTGC CAACCATGCT GCAGGTCTAT GCAGACTACG AGAGCAACCC TCTTCTGAGG 
CAGGGCATTG AGTTTTGCTG CCGGCAGTTT TACATCCTCC ATCGAAAACC CTTTATCCTG 
CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC GAGTTCACAA CCAGCACCAG TACCGGTCTT 
TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC GACCTGCTGG TCTCTCTAGA AGGTGAAACC 
CAGGATGCCC TGGATGCTCT GGAGCTGGTA AAAGCTGAGA AGCCTCTACG CTCTCTGGAT 
TTCTGTTATG GTAATGAGGA CCTGACCTTT TCTATCAGCG AGGCCATCAA GCTGTGCGTC 
ACTGTGGTTG CATATGCCCC CGAATCCTTC AGGAGTCTCC AGATGCTGAT GGTGCTGGAG 
GCCTTGGTTC CCTGCTACCT CCAGAAACTG AAGAGCAACA CAGTTACGAT GGAGTCTGCT 
TCGGCTGCCA GAGATGAGAT TGCGGCCATC GCTGCCCTAG CTACGTCTCT GCAGGCCCTG 
CTCTACAGCT CTGAAACCCT CACAAGGCCC ATGACGGCTC CCCAGATATC TCGCTGTGAT 
CAGGGCCACA AGGGCGGCAC TACAGCTAAC CACGCCATGT CCGGAGGGGT CAACACCAGG 
GACAATCTTC ACCTGCTGGA GGAGGGCCAG GGCATGCCAA GGGAAGAGCT GGACGAGCGC 
ATTGCCAGGG AGGAGTTCCG CCGTCCACGG GAGTCCCTGT TGAACATCTG CACCGAGTTT 
TACAAACACT GCGGGCCACG GCTCAAAATC CTGCAGAACG TTGCAGGCGA GCCGCGGGTC 
ACAGCTCTGG AGCTACTGGA CATCAAGTCC CACATGAGGC TGGCAGAGAT CGCCCACGCC 
CTGCTGAAGC TGGCGCCCTA CGACACCCTG ACCATGGAGA GCCGAGGGCT GCGGCGTTAC 
ATCATGGAGA TGCTGCCCAT CACCGACTGG TCGTCCGAGG CCGTCCGGCC TGCCCTCATC 
CTCATCCTCA AGAGGCTGGA CCGCATGTTC AACAAGATCC ACAAGATGCC CACGCTCAGG 
TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GAAACTTTAT TGGACTGTGA AGCGCATGGA 
GACCAAAAGA GGTCAGTCAC CTTTACAGAG AATCAAGGCC AGCAGGCATC CACGGGGGCA 
AGCAAGCCTC CAACTCCCAG CACTGTGGAC CCACCGGGCG AAGGCAGAGG GACGAGTCCT 
GCCCCATCAA AGTCCCCAAC CCTGGAAGTA CCCCCCACCT CTTCAGCCAT GATCTTGGCT 
GACCTGCACG GACCTACAAA TGCACAGGTC CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA 
GAGCAGTGGG GGCTACGCAG CAGCCTCTCC CCTCCCCCAT CCATCTCCCG GCCTTCCACT 
CCAACACCCC CATCCCGAAC CTGCTCCCCT CTCCCCCTCT CCAGAACCTG CTCCCCTCTC 
CCTTTGTCCC GCACCTCCTC TCCTCTTCCA CCGCCACTCC CAGTGCTGAG CACAGCACCC 
GCTCCGGGTC CACCGCCGCC ACCTCCATTA CCACCAACTC ATCTTGCTGC TGCTCCTCCT 
CCTCCACCAC CCCCGGGGCC TTCTAGGATC AGAGAGAGCC CCGGGGACGA GGACACAACC 
GCCCTGCTGC CGCGCAGCGA CACCCTGCTG CACCTCAGCG AGGACGATGG CACCGAGAAC 
CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC 
CTCCCCCTCT CACCCGTCGA CCTGGACCTG GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562576.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562633.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.

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CloneID	OMa370047
Clone ID Related Accession (Same CDS sequence)	XM_004562576.4
Accession Version	XM_004562576.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562576.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAT GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562633.2
CDS	397..10866
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562576.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562576.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAT GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562577.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562634.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.

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CloneID	OMa370049
Clone ID Related Accession (Same CDS sequence)	XM_004562577.5
Accession Version	XM_004562577.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562577.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562634.2
CDS	398..10858
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562577.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562577.5

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562578.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562635.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.

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CloneID	OMa370051
Clone ID Related Accession (Same CDS sequence)	XM_004562578.4
Accession Version	XM_004562578.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562578.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGTGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562635.2
CDS	398..10858
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562578.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562578.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGTGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562579.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562636.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.

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CloneID	OMa370052
Clone ID Related Accession (Same CDS sequence)	XM_004562579.4
Accession Version	XM_004562579.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562579.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG 
AGACAGGTGG AGTGGGAGGC GGCCAGTAGC CTGATCGAGG GGATCTGCCT GACCTTGCAG 
CGACAGCCGA TCATCTCGTT CCTGCCTCAC CTGCGCTCGC TTATCAACGT CTGCGTCAAC 
CTGGTGATGG GTGTGGTCGG CCCCTCCAGT GTGGCGGACG GGCTGCCACT CCTCCACCTC 
AGCCCTTACC TGTCTCCGCC TCTGCCCTTT AGCACCGCCG TGGTCCGATT GGTTGCTCTG 
CAGATTCAGG CCTTGAAGGA AGACTTTCCC CTCAGTCACG TGATCTCACC TTTCACCAAT 
CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC CTGCTCATTC CCTTCGTGTT AACTGTAGGC 
TCCGGGAGCA AAGACAGCCC CCACCTGGAG CAGCCGGAGG TCTTCCTGCT CCTACAGACA 
GTCATCAACA TCCTGCTTCC CCCTCGTATC ATCTCCACAT CCCGCACCAA GAACTTTATG 
CTTGACGCCT CTCCGGCTCA CTGCTCCACG CCGGGTGACC CTGGGAAAGA TCTGCGTAGG 
GAGGGATTAG CAGAGTCCAC GAGCCAGGCT GCTTATTTAG CTCTGAAGGT GGTGCTGGTT 
TGCTTCGAGC GCTCGCTGGG GAACCAGTGG TATCGGCTGA GCCTGCAGGT GAAGGAGATG 
GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC TGGGACTTCA TCGACTTTAT CGTCCGAACC 
CGGATCCCTA TTTTTGTCCT GCTCAGACCC TTTATACAGT GCAAGTTGCT GACCCAGCCG 
GCCGATTCCC AGGAAGAGAT CACAGCGCGC CACCACATCG CTGATCAGCT GGAGCGGCGC 
TTCATCCCAC GACCTCTCTG CAAGAGCTCA CTGTTTGCAG AGTTCAACAA TGAGCTCAAG 
ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA GCATATCAGG GCAAGACATC CATCAGCACG 
GTGGGCACCT CCACGTCAGC CTACCGCCTC AGTTTGGCCA CAATGTCGCG ATCCAACACA 
GGCACTGGAA CAGTTTGGGA GCAGGACAGC CAACCGTCAC GGCAGCCCTC ACAGGACACG 
CTCAGCCGTA CCGACGAGGA CGATGAAGAG ATTGACTCCA TGAGCATTCC CAGCGTGGTG 
AGCGAGCACG AAGCCTTCAT GCCCAGGGTG ATGTCACAGC GCCGTTTCTC CAGCCACGCC 
ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC CCCCGCACCA CCATGTTGCC CAGCCACAGT 
GAACCCAATG TGCTAGATGA GTCCCAGGGT CTTCTGCAGG AGGGTAACCT CTCTAGGGTG 
GCCAGTGTGC AGAGCGAACC AGGTCAGAAC CTCCTGATCC AGCCCCCGTT AGGAAGAAAG 
CGAGGACTCA GACAGATGCG ACGCCCCCTG CTGTCCATTC CAAAAACCGA ACCACGCGGT 
CGATCTGGAG CGAGGCTTTC TACAACGCGC AGGAGCATCC AGCCCAAGAA CAAACCACTG 
GAAACTTTAT TGGACTGTGA AGCGCATGGA GACCAAAAGA GGTCAGTCAC CTTTACAGAG 
AATCAAGGCC AGCAGGCATC CACGGGGGCA AGCAAGCCTC CAACTCCCAG CACTGTGGAC 
CCACCGGGCG AAGGCAGAGG GACGAGTCCT GCCCCATCAA AGTCCCCAAC CCTGGAAGTA 
CCCCCCACCT CTTCAGCCAT GATCTTGGCT GACCTGCACG GACCTACAAA TGCACAGGTC 
CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA GAGCAGTGGG GGCTACGCAG CAGCCTCTCC 
CCTCCCCCAT CCATCTCCCG GCCTTCCACT CCAACACCCC CATCCCGAAC CTGCTCCCCT 
CTCCCCCTCT CCAGAACCTG CTCCCCTCTC CCTTTGTCCC GCACCTCCTC TCCTCTTCCA 
CCGCCACTCC CAGTGCTGAG CACAGCACCC GCTCCGGGTC CACCGCCGCC ACCTCCATTA 
CCACCAACTC ATCTTGCTGC TGCTCCTCCT CCTCCACCAC CCCCGGGGCC TTCTAGGATC 
AGAGAGAGCC CCGGGGACGA GGACACAACC GCCCTGCTGC CGCGCAGCGA CACCCTGCTG 
CACCTCAGCG AGGACGATGG CACCGAGAAC CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT 
CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC CTCCCCCTCT CACCCGTCGA CCTGGACCTG 
GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562636.2
CDS	397..10854
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562579.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562579.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG TGCGCTCGCC CTCAGGCTTT ATGCAGCAGG 
AGACAGGTGG AGTGGGAGGC GGCCAGTAGC CTGATCGAGG GGATCTGCCT GACCTTGCAG 
CGACAGCCGA TCATCTCGTT CCTGCCTCAC CTGCGCTCGC TTATCAACGT CTGCGTCAAC 
CTGGTGATGG GTGTGGTCGG CCCCTCCAGT GTGGCGGACG GGCTGCCACT CCTCCACCTC 
AGCCCTTACC TGTCTCCGCC TCTGCCCTTT AGCACCGCCG TGGTCCGATT GGTTGCTCTG 
CAGATTCAGG CCTTGAAGGA AGACTTTCCC CTCAGTCACG TGATCTCACC TTTCACCAAT 
CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC CTGCTCATTC CCTTCGTGTT AACTGTAGGC 
TCCGGGAGCA AAGACAGCCC CCACCTGGAG CAGCCGGAGG TCTTCCTGCT CCTACAGACA 
GTCATCAACA TCCTGCTTCC CCCTCGTATC ATCTCCACAT CCCGCACCAA GAACTTTATG 
CTTGACGCCT CTCCGGCTCA CTGCTCCACG CCGGGTGACC CTGGGAAAGA TCTGCGTAGG 
GAGGGATTAG CAGAGTCCAC GAGCCAGGCT GCTTATTTAG CTCTGAAGGT GGTGCTGGTT 
TGCTTCGAGC GCTCGCTGGG GAACCAGTGG TATCGGCTGA GCCTGCAGGT GAAGGAGATG 
GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC TGGGACTTCA TCGACTTTAT CGTCCGAACC 
CGGATCCCTA TTTTTGTCCT GCTCAGACCC TTTATACAGT GCAAGTTGCT GACCCAGCCG 
GCCGATTCCC AGGAAGAGAT CACAGCGCGC CACCACATCG CTGATCAGCT GGAGCGGCGC 
TTCATCCCAC GACCTCTCTG CAAGAGCTCA CTGTTTGCAG AGTTCAACAA TGAGCTCAAG 
ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA GCATATCAGG GCAAGACATC CATCAGCACG 
GTGGGCACCT CCACGTCAGC CTACCGCCTC AGTTTGGCCA CAATGTCGCG ATCCAACACA 
GGCACTGGAA CAGTTTGGGA GCAGGACAGC CAACCGTCAC GGCAGCCCTC ACAGGACACG 
CTCAGCCGTA CCGACGAGGA CGATGAAGAG ATTGACTCCA TGAGCATTCC CAGCGTGGTG 
AGCGAGCACG AAGCCTTCAT GCCCAGGGTG ATGTCACAGC GCCGTTTCTC CAGCCACGCC 
ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC CCCCGCACCA CCATGTTGCC CAGCCACAGT 
GAACCCAATG TGCTAGATGA GTCCCAGGGT CTTCTGCAGG AGGGTAACCT CTCTAGGGTG 
GCCAGTGTGC AGAGCGAACC AGGTCAGAAC CTCCTGATCC AGCCCCCGTT AGGAAGAAAG 
CGAGGACTCA GACAGATGCG ACGCCCCCTG CTGTCCATTC CAAAAACCGA ACCACGCGGT 
CGATCTGGAG CGAGGCTTTC TACAACGCGC AGGAGCATCC AGCCCAAGAA CAAACCACTG 
GAAACTTTAT TGGACTGTGA AGCGCATGGA GACCAAAAGA GGTCAGTCAC CTTTACAGAG 
AATCAAGGCC AGCAGGCATC CACGGGGGCA AGCAAGCCTC CAACTCCCAG CACTGTGGAC 
CCACCGGGCG AAGGCAGAGG GACGAGTCCT GCCCCATCAA AGTCCCCAAC CCTGGAAGTA 
CCCCCCACCT CTTCAGCCAT GATCTTGGCT GACCTGCACG GACCTACAAA TGCACAGGTC 
CCTCAAGCAT TAAGCAGCAA GGAGAAGAGA GAGCAGTGGG GGCTACGCAG CAGCCTCTCC 
CCTCCCCCAT CCATCTCCCG GCCTTCCACT CCAACACCCC CATCCCGAAC CTGCTCCCCT 
CTCCCCCTCT CCAGAACCTG CTCCCCTCTC CCTTTGTCCC GCACCTCCTC TCCTCTTCCA 
CCGCCACTCC CAGTGCTGAG CACAGCACCC GCTCCGGGTC CACCGCCGCC ACCTCCATTA 
CCACCAACTC ATCTTGCTGC TGCTCCTCCT CCTCCACCAC CCCCGGGGCC TTCTAGGATC 
AGAGAGAGCC CCGGGGACGA GGACACAACC GCCCTGCTGC CGCGCAGCGA CACCCTGCTG 
CACCTCAGCG AGGACGATGG CACCGAGAAC CCGCTTCTCA CCCCGCTGCT GTCCCCTCCT 
CCCCCCCGCC GGTCGCCCCG CCGCTCTCCC CTCCCCCTCT CACCCGTCGA CCTGGACCTG 
GATGAGTCCC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922258.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777712.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.

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CloneID	OMa370053
Clone ID Related Accession (Same CDS sequence)	XM_012922258.3
Accession Version	XM_012922258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGAAGAAGTG 
GTGAACCTGG CATCACGACG TCTGTCTGTC AGCCCATCCT GCGCCTCCAG CAACTCCCAC 
AGGAACTACT CTTTCCGCCG GGGCTCTGTA TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA 
GATGAAGAAA ACACAACAGA ACACACACCC ACTCACCACA TGCTACAGCC ACCTCAAGCT 
GTCTTCCCAG CATGCATCTG TGCTGCCGTC CTACCAATAG TGCACCTCAT GGAAGACGGT 
GAAGTTAGAG AGGATGGCGT GGCTGTGAGT GCTGTTGCCC AGCAAATCCT CTGGAACTGC 
CTCATTGAAG ATCCAGCTCT CGTTCTCCGC CACTTCCTGG AAAAGCTGAC AGTTAGCAAC 
AGACAGGACG AGCTGATGTA CATGCTTAGG AAGCTCCTGC TCAACATTGG AGATCTGCCA 
GCCCAGACCT CTCACATCCT TTTTAACTAC CTGGTGGGCC TGATCATGTA TTTTGTGCGG 
ACTCCCTGTG AATGGGGCAT GGACGCCATC TCGGCCACGC TGACCTTCCT CTGGGAGGTG 
GTCGGCTACG TGGAGGGGCT CTTCTTTAAA GACCTGAAGC AGACCATGAA GAAAGAGCAG 
TGTGAGGTCA AACTGCTGGT CACAGCATCG ATGCCAGGAA CCAAAACGCT GGTGGTGCAC 
GGGCAGAATG AATGTGACAT CCCAACCCAG CTACCAGTCC ACGAAGACAC TCAGTTTGAA 
GCCCTGCTAA AGGAGTGTCT GGAATTTTTC AACATTCCCG AGGCCAGATC GGCACACTAC 
TTCCTCATGG ATAAACGATG GAACCTCATC CATTATTCAA AGACATATGT TAGAGACATC 
TACCCGTTCC GAAGGTCAGT CTCTCCTCAG CTCAACCTGG TCCACATGTT GCCTGAGAAA 
GGACAGGAGC TCATCCAGAA ACAGGTGTTT TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC 
CTCTTCCTCA TCTCCCTCAC ACAACACATG CCAGCTGTGC ACAAGCAGTC CCACGTCTCC 
CTGCTGCAGG AGGACCTGCT TCGCCTGCCC TCCTTCCCCC GCACCGCCAT CGATGCAGAG 
TTCTCCCTCT TTAATGAGCC TCAAGGAAAA GAGCTGTTTG GCTTGGACAC TCTCCACAAG 
GTGTTGTGGA TCAAACTGTT GGAGGAAATG TTTCTGGGCA TGCCCAGTGA GTACCCGTGG 
GGCGATGAGA TGATGCTGTT CCTTAACGTC TTCAACGGGG CCCTTCTGCT GCACCCCGAG 
GACAGCTCCC TGCTGAGGCA GTACACGGCG ACCGCCATCA ACACCGCCGT TCACTTTAAC 
CATCTGTTCT CCCTGAGCGG GTACCAGTGG ATGCTGCCAA CCATGCTGCA GGTCTATGCA 
GACTACGAGA GCAACCCTCT TCTGAGGCAG GGCATTGAGT TTTGCTGCCG GCAGTTTTAC 
ATCCTCCATC GAAAACCCTT TATCCTGCAG CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG 
TTCACAACCA GCACCAGTAC CGGTCTTTCT AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC 
CTGCTGGTCT CTCTAGAAGG TGAAACCCAG GATGCCCTGG ATGCTCTGGA GCTGGTAAAA 
GCTGAGAAGC CTCTACGCTC TCTGGATTTC TGTTATGGTA ATGAGGACCT GACCTTTTCT 
ATCAGCGAGG CCATCAAGCT GTGCGTCACT GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG 
AGTCTCCAGA TGCTGATGGT GCTGGAGGCC TTGGTTCCCT GCTACCTCCA GAAACTGAAG 
AGCAACACAG TTACGATGGA GTCTGCTTCG GCTGCCAGAG ATGAGATTGC GGCCATCGCT 
GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC TACAGCTCTG AAACCCTCAC AAGGCCCATG 
ACGGCTCCCC AGATATCTCG CTGTGATCAG GGCCACAAGG GCGGCACTAC AGCTAACCAC 
GCCATGTCCG GAGGGGTCAA CACCAGGGAC AATCTTCACC TGCTGGAGGA GGGCCAGGGC 
ATGCCAAGGG AAGAGCTGGA CGAGCGCATT GCCAGGGAGG AGTTCCGCCG TCCACGGGAG 
TCCCTGTTGA ACATCTGCAC CGAGTTTTAC AAACACTGCG GGCCACGGCT CAAAATCCTG 
CAGAACGTTG CAGGCGAGCC GCGGGTCACA GCTCTGGAGC TACTGGACAT CAAGTCCCAC 
ATGAGGCTGG CAGAGATCGC CCACGCCCTG CTGAAGCTGG CGCCCTACGA CACCCTGACC 
ATGGAGAGCC GAGGGCTGCG GCGTTACATC ATGGAGATGC TGCCCATCAC CGACTGGTCG 
TCCGAGGCCG TCCGGCCTGC CCTCATCCTC ATCCTCAAGA GGCTGGACCG CATGTTCAAC 
AAGATCCACA AGATGCCCAC GCTCAGGTGC GCTCGCCCTC AGGCTTTATG CAGCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTTCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGATGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777712.2
CDS	397..10851
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922258.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922258.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGAAGAAGTG 
GTGAACCTGG CATCACGACG TCTGTCTGTC AGCCCATCCT GCGCCTCCAG CAACTCCCAC 
AGGAACTACT CTTTCCGCCG GGGCTCTGTA TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA 
GATGAAGAAA ACACAACAGA ACACACACCC ACTCACCACA TGCTACAGCC ACCTCAAGCT 
GTCTTCCCAG CATGCATCTG TGCTGCCGTC CTACCAATAG TGCACCTCAT GGAAGACGGT 
GAAGTTAGAG AGGATGGCGT GGCTGTGAGT GCTGTTGCCC AGCAAATCCT CTGGAACTGC 
CTCATTGAAG ATCCAGCTCT CGTTCTCCGC CACTTCCTGG AAAAGCTGAC AGTTAGCAAC 
AGACAGGACG AGCTGATGTA CATGCTTAGG AAGCTCCTGC TCAACATTGG AGATCTGCCA 
GCCCAGACCT CTCACATCCT TTTTAACTAC CTGGTGGGCC TGATCATGTA TTTTGTGCGG 
ACTCCCTGTG AATGGGGCAT GGACGCCATC TCGGCCACGC TGACCTTCCT CTGGGAGGTG 
GTCGGCTACG TGGAGGGGCT CTTCTTTAAA GACCTGAAGC AGACCATGAA GAAAGAGCAG 
TGTGAGGTCA AACTGCTGGT CACAGCATCG ATGCCAGGAA CCAAAACGCT GGTGGTGCAC 
GGGCAGAATG AATGTGACAT CCCAACCCAG CTACCAGTCC ACGAAGACAC TCAGTTTGAA 
GCCCTGCTAA AGGAGTGTCT GGAATTTTTC AACATTCCCG AGGCCAGATC GGCACACTAC 
TTCCTCATGG ATAAACGATG GAACCTCATC CATTATTCAA AGACATATGT TAGAGACATC 
TACCCGTTCC GAAGGTCAGT CTCTCCTCAG CTCAACCTGG TCCACATGTT GCCTGAGAAA 
GGACAGGAGC TCATCCAGAA ACAGGTGTTT TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC 
CTCTTCCTCA TCTCCCTCAC ACAACACATG CCAGCTGTGC ACAAGCAGTC CCACGTCTCC 
CTGCTGCAGG AGGACCTGCT TCGCCTGCCC TCCTTCCCCC GCACCGCCAT CGATGCAGAG 
TTCTCCCTCT TTAATGAGCC TCAAGGAAAA GAGCTGTTTG GCTTGGACAC TCTCCACAAG 
GTGTTGTGGA TCAAACTGTT GGAGGAAATG TTTCTGGGCA TGCCCAGTGA GTACCCGTGG 
GGCGATGAGA TGATGCTGTT CCTTAACGTC TTCAACGGGG CCCTTCTGCT GCACCCCGAG 
GACAGCTCCC TGCTGAGGCA GTACACGGCG ACCGCCATCA ACACCGCCGT TCACTTTAAC 
CATCTGTTCT CCCTGAGCGG GTACCAGTGG ATGCTGCCAA CCATGCTGCA GGTCTATGCA 
GACTACGAGA GCAACCCTCT TCTGAGGCAG GGCATTGAGT TTTGCTGCCG GCAGTTTTAC 
ATCCTCCATC GAAAACCCTT TATCCTGCAG CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG 
TTCACAACCA GCACCAGTAC CGGTCTTTCT AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC 
CTGCTGGTCT CTCTAGAAGG TGAAACCCAG GATGCCCTGG ATGCTCTGGA GCTGGTAAAA 
GCTGAGAAGC CTCTACGCTC TCTGGATTTC TGTTATGGTA ATGAGGACCT GACCTTTTCT 
ATCAGCGAGG CCATCAAGCT GTGCGTCACT GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG 
AGTCTCCAGA TGCTGATGGT GCTGGAGGCC TTGGTTCCCT GCTACCTCCA GAAACTGAAG 
AGCAACACAG TTACGATGGA GTCTGCTTCG GCTGCCAGAG ATGAGATTGC GGCCATCGCT 
GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC TACAGCTCTG AAACCCTCAC AAGGCCCATG 
ACGGCTCCCC AGATATCTCG CTGTGATCAG GGCCACAAGG GCGGCACTAC AGCTAACCAC 
GCCATGTCCG GAGGGGTCAA CACCAGGGAC AATCTTCACC TGCTGGAGGA GGGCCAGGGC 
ATGCCAAGGG AAGAGCTGGA CGAGCGCATT GCCAGGGAGG AGTTCCGCCG TCCACGGGAG 
TCCCTGTTGA ACATCTGCAC CGAGTTTTAC AAACACTGCG GGCCACGGCT CAAAATCCTG 
CAGAACGTTG CAGGCGAGCC GCGGGTCACA GCTCTGGAGC TACTGGACAT CAAGTCCCAC 
ATGAGGCTGG CAGAGATCGC CCACGCCCTG CTGAAGCTGG CGCCCTACGA CACCCTGACC 
ATGGAGAGCC GAGGGCTGCG GCGTTACATC ATGGAGATGC TGCCCATCAC CGACTGGTCG 
TCCGAGGCCG TCCGGCCTGC CCTCATCCTC ATCCTCAAGA GGCTGGACCG CATGTTCAAC 
AAGATCCACA AGATGCCCAC GCTCAGGTGC GCTCGCCCTC AGGCTTTATG CAGCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTTCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGATGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922259.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777713.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.

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CloneID	OMa370054
Clone ID Related Accession (Same CDS sequence)	XM_012922259.3
Accession Version	XM_012922259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGCGCATG GAGACCAAAA GAGGTCAGTC ACCTTTACAG AGAATCAAGG CCAGCAGGCA 
TCCACGGGGG CAAGCAAGCC TCCAACTCCC AGCACTGTGG ACCCACCGGG CGAAGGCAGA 
GGGACGAGTC CTGCCCCATC AAAGTCCCCA ACCCTGGAAG TACCCCCCAC CTCTTCAGCC 
ATGATCTTGG CTGACCTGCA CGGACCTACA AATGCACAGG TCCCTCAAGC ATTAAGCAGC 
AAGGAGAAGA GAGAGCAGTG GGGGCTACGC AGCAGCCTCT CCCCTCCCCC ATCCATCTCC 
CGGCCTTCCA CTCCAACACC CCCATCCCGA ACCTGCTCCC CTCTCCCCCT CTCCAGAACC 
TGCTCCCCTC TCCCTTTGTC CCGCACCTCC TCTCCTCTTC CACCGCCACT CCCAGTGCTG 
AGCACAGCAC CCGCTCCGGG TCCACCGCCG CCACCTCCAT TACCACCAAC TCATCTTGCT 
GCTGCTCCTC CTCCTCCACC ACCCCCGGGG CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC 
GAGGACACAA CCGCCCTGCT GCCGCGCAGC GACACCCTGC TGCACCTCAG CGAGGACGAT 
GGCACCGAGA ACCCGCTTCT CACCCCGCTG CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC 
CGCCGCTCTC CCCTCCCCCT CTCACCCGTC GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777713.2
CDS	397..10836
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922259.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922259.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGCGCATG GAGACCAAAA GAGGTCAGTC ACCTTTACAG AGAATCAAGG CCAGCAGGCA 
TCCACGGGGG CAAGCAAGCC TCCAACTCCC AGCACTGTGG ACCCACCGGG CGAAGGCAGA 
GGGACGAGTC CTGCCCCATC AAAGTCCCCA ACCCTGGAAG TACCCCCCAC CTCTTCAGCC 
ATGATCTTGG CTGACCTGCA CGGACCTACA AATGCACAGG TCCCTCAAGC ATTAAGCAGC 
AAGGAGAAGA GAGAGCAGTG GGGGCTACGC AGCAGCCTCT CCCCTCCCCC ATCCATCTCC 
CGGCCTTCCA CTCCAACACC CCCATCCCGA ACCTGCTCCC CTCTCCCCCT CTCCAGAACC 
TGCTCCCCTC TCCCTTTGTC CCGCACCTCC TCTCCTCTTC CACCGCCACT CCCAGTGCTG 
AGCACAGCAC CCGCTCCGGG TCCACCGCCG CCACCTCCAT TACCACCAAC TCATCTTGCT 
GCTGCTCCTC CTCCTCCACC ACCCCCGGGG CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC 
GAGGACACAA CCGCCCTGCT GCCGCGCAGC GACACCCTGC TGCACCTCAG CGAGGACGAT 
GGCACCGAGA ACCCGCTTCT CACCCCGCTG CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC 
CGCCGCTCTC CCCTCCCCCT CTCACCCGTC GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562580.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562637.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.

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CloneID	OMa370055
Clone ID Related Accession (Same CDS sequence)	XM_004562580.4
Accession Version	XM_004562580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562580.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGGGTGGCCA GTGTGCAGAG CGAACCAGGT 
CAGAACCTCC TGATCCAGCC CCCGTTAGGA AGAAAGCGAG GACTCAGACA GATGCGACGC 
CCCCTGCTGT CCATTCCAAA AACCGAACCA CGCGGTCGAT CTGGAGCGAG GCTTTCTACA 
ACGCGCAGGA GCATCCAGCC CAAGAACAAA CCACTGGAAA CTTTATTGGA CTGTGAAGCG 
CATGGAGACC AAAAGAGGTC AGTCACCTTT ACAGAGAATC AAGGCCAGCA GGCATCCACG 
GGGGCAAGCA AGCCTCCAAC TCCCAGCACT GTGGACCCAC CGGGCGAAGG CAGAGGGACG 
AGTCCTGCCC CATCAAAGTC CCCAACCCTG GAAGTACCCC CCACCTCTTC AGCCATGATC 
TTGGCTGACC TGCACGGACC TACAAATGCA CAGGTCCCTC AAGCATTAAG CAGCAAGGAG 
AAGAGAGAGC AGTGGGGGCT ACGCAGCAGC CTCTCCCCTC CCCCATCCAT CTCCCGGCCT 
TCCACTCCAA CACCCCCATC CCGAACCTGC TCCCCTCTCC CCCTCTCCAG AACCTGCTCC 
CCTCTCCCTT TGTCCCGCAC CTCCTCTCCT CTTCCACCGC CACTCCCAGT GCTGAGCACA 
GCACCCGCTC CGGGTCCACC GCCGCCACCT CCATTACCAC CAACTCATCT TGCTGCTGCT 
CCTCCTCCTC CACCACCCCC GGGGCCTTCT AGGATCAGAG AGAGCCCCGG GGACGAGGAC 
ACAACCGCCC TGCTGCCGCG CAGCGACACC CTGCTGCACC TCAGCGAGGA CGATGGCACC 
GAGAACCCGC TTCTCACCCC GCTGCTGTCC CCTCCTCCCC CCCGCCGGTC GCCCCGCCGC 
TCTCCCCTCC CCCTCTCACC CGTCGACCTG GACCTGGATG AGTCCCACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562637.2
CDS	398..10831
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562580.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562580.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGGGTGGCCA GTGTGCAGAG CGAACCAGGT 
CAGAACCTCC TGATCCAGCC CCCGTTAGGA AGAAAGCGAG GACTCAGACA GATGCGACGC 
CCCCTGCTGT CCATTCCAAA AACCGAACCA CGCGGTCGAT CTGGAGCGAG GCTTTCTACA 
ACGCGCAGGA GCATCCAGCC CAAGAACAAA CCACTGGAAA CTTTATTGGA CTGTGAAGCG 
CATGGAGACC AAAAGAGGTC AGTCACCTTT ACAGAGAATC AAGGCCAGCA GGCATCCACG 
GGGGCAAGCA AGCCTCCAAC TCCCAGCACT GTGGACCCAC CGGGCGAAGG CAGAGGGACG 
AGTCCTGCCC CATCAAAGTC CCCAACCCTG GAAGTACCCC CCACCTCTTC AGCCATGATC 
TTGGCTGACC TGCACGGACC TACAAATGCA CAGGTCCCTC AAGCATTAAG CAGCAAGGAG 
AAGAGAGAGC AGTGGGGGCT ACGCAGCAGC CTCTCCCCTC CCCCATCCAT CTCCCGGCCT 
TCCACTCCAA CACCCCCATC CCGAACCTGC TCCCCTCTCC CCCTCTCCAG AACCTGCTCC 
CCTCTCCCTT TGTCCCGCAC CTCCTCTCCT CTTCCACCGC CACTCCCAGT GCTGAGCACA 
GCACCCGCTC CGGGTCCACC GCCGCCACCT CCATTACCAC CAACTCATCT TGCTGCTGCT 
CCTCCTCCTC CACCACCCCC GGGGCCTTCT AGGATCAGAG AGAGCCCCGG GGACGAGGAC 
ACAACCGCCC TGCTGCCGCG CAGCGACACC CTGCTGCACC TCAGCGAGGA CGATGGCACC 
GAGAACCCGC TTCTCACCCC GCTGCTGTCC CCTCCTCCCC CCCGCCGGTC GCCCCGCCGC 
TCTCCCCTCC CCCTCTCACC CGTCGACCTG GACCTGGATG AGTCCCACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562581.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562638.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.

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CloneID	OMa370056
Clone ID Related Accession (Same CDS sequence)	XM_004562581.4
Accession Version	XM_004562581.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562638.2
CDS	398..10828
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562581.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562581.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_023153469.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009237.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.

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CloneID	OMa370057
Clone ID Related Accession (Same CDS sequence)	XM_023153469.2
Accession Version	XM_023153469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGACA AGGCGGGCTT CGGAAACAAC 
TTCGCCACAG GAGACAACAA ATCTGTAGCC CAGAATATGG AGGCCGTGGT GGTGGGCTGC 
ATGTTCAAGT CCCTCATCAC CCGCTGCGCC TCAACCACAC ATGAGCTGCA CAGCCCCGAG 
AACCTGGGTC TGTACTGTGA CATCCGCCAG CTGGTCCAGT TCATTAAAGA GGCCCACGGA 
AACGTGTTCC GCAGGGTGGC ACTGAGCGCG CTCCTGGACA GCGCGGAGAA GGTCACCGCC 
ACCAAGAAAC CTGATGAGAA GGAGGAGACC AAACAGCCCG GTCCCAGGAG GTCAGAGGCG 
GGTGTGTCCG GGGAGAAGGG TCAGGTGTCC AGTACTGCAG ACGAGTGTCG CAGCTACATC 
TCCAGTAGAC CCCCCCAGAC ACCCGAACAT GAAGAACAGA TCCCGGGTGC TCTGCTGGGC 
AGGAAGGATT TTTGGAGGAA GATGTTCAAG TCGCAGAGCG CCGCCAGTGA CACCAGCAGC 
CAGTCGGAAC AAGACACCTC AGAGTGCACC ACCGCACACT CTGGCACCAC CACCGACCGC 
CGCTCACGAT CCAGATCCCG TCGCATTTCG CTTCGCAAGA AGTTGAAGCT GCCTATCGGG 
AACTGGTTGA AGCGCTCCTC TCTCTCTGGA CTGACTGACG GCGTCGAGGA CTTGCTGGAT 
ATCAGCTCGG TGGATCGACT CTCCTTCATA CGTCAGAGTT CAAAGGTGAA GTTCACTAGT 
GCAGTGAAGC TATCTGAAGG TGGCGCTGTA GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG 
AATTTCTTCA AGCGGCTCGG TAAATGGAGG TCTGGCAGGA GGAATCCATC CAAGCTTCAC 
CACACAGAAG AGAAAGATGG ACCTCACGGT TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG 
GCCATGAAGA ACAAGAATGT AGTGAATCTG GGCGCCATCC GACAGGGTAT GAAACGCTTC 
CAGTTTCTCC TGAACTGCTG CGAGCCAGGA ACCATACCAG ATGCCTCCAT CCTCGCCGCT 
GCTCTGGACC TCGAAGCCCC AGTTGTGGCC AGGGCCTCCC TTTTCTTGGA ATGTGCCAGA 
TTTGTCCACC GGTGTAATCG TGGCAACTGG CCCGAATGGA TGAAGGGCCA CCACGTAAAC 
ATCACCAAGA GAGGGCTGTC CAGGGGCCGA TCGCCTATTG TGGGCAATAA AAGAAACCAG 
AAGCTCCAGT GGAACGCTGC CAAGCATTTC TGCCAGTGGG GAGACGCGAT TGGCACGAGG 
CTCAGCGAAC TCTGCCACTC GGACAGTGAG AGCCCCGCCA ACATCCTGGG TTATATTTAC 
GATGAAGAGA CCAAACGCAG GATGAGGAAA GAAGACGAGG AAGAAGACTA TTTAGATGAC 
AACACGGTCA ATCCTACAAA ATGTGGCTGC CCCTTCGCAC TGAAGATGGC CGCTTGCCAG 
CTGTTGTTGG AGATCACCAC TTTCCTGCGA GAGACCTTCC CCTGCTTACC ACGACCGCGC 
ACCGAACCGC TTGTGGATCT GGACAGCTGC CGCCTGCGTC TGGACCCAGA GCTCGGCCGC 
CATCGCTACG AGAGGAAGAT CAGCTTTGCG GGCATCCTCG ACGACGAAGA CGGGCACGAT 
TCCCTCAACA GCAGCAGCCA CACTCTGAAG TCTGACACCA CCTGTGATGA TAAGAAGACC 
CAGGAGGCTC AGGACAATCT CTCCCCACTC CCCTGCTCTC CAGCACCAGT GCGGAAGATT 
CGTATCGGAG GCTCGCGCCT GCTTCAGATC AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG 
GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG GGGAGTCTGA AGAGCACCAA GCTGTCTCGG 
CAGGACTCTG AGTCTGAGAA CGAAGAAGGC CTGCTGTCAC AAACGCAGAG CAGGGACACA 
GTTACTGACA TCGGCAGTCC CTTCAGCACC AGTGAACCCA GCATCGAGCC AGAAGGTCAG 
AGCTCAGGAG GGACGGAGGA CAACTACCAC CGCAACATGT CCTGGCTTCA CATTATGATC 
CTGCTGTGCA ACCAGCAGAG TTTCATCTGT ACCCACATAG ACTTCTGCCA CCCACGCTGC 
TACCAACACC ACAGCCGCTC CTGCGCCCGT CTGGTCCGTG CCATCAAGCT CGTGTACGGG 
GAGACGGTGG ACAGCCTGAG GGAGGACAGC GCCTCCTCTG GTAACATCGG AGGACGTGTG 
AAGAAAAACA AAGAGTGTTC AGACAAGTCT TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA 
CCCACTGACT CCAACACAGA AGGGAAGAAG GATACAGGCA TGCTCAAGTA CATCCGCAAT 
CAGGTGATGA GTTTGTCACC AGCTCCTCTT TCACTGCTGA TCAAAGCAGC TCCTATCTTG 
ACTGACGACA TGTACGGAGA CATCCAGCCC GCAGCCTGGG AACTCCTACT CAGTGTGGAT 
GAACATATGG CAGCCGCTGC TGCTGCCTTG TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT 
GCAGTGACCG AAATGATGAT GGCAGAGTTC CAGCACCCTG AGGCCTGCCA GCGCATTAAC 
TCCATCCTGA AGTTTTACAC TCTGTGGCGT TTTCGCTACC AAGTGTGGCC TCGCATGGAG 
GAAGGAGCCC AGCAGATCTT TAAGATCCCT CCACCCAGCA TTAACTTCAC TCTCCCTTCT 
CCAATACTGG GCATGCCCTG TGTCCCCATA TTTGACCCTC CCTGGGTGCC CCAGAACACT 
GGCAGCGTCC AGGATCCAAT CAACGAAGAC CAGTCTTACC CCACATTAAT TCAGTCAGTC 
CATGCCATAA AATCCTTCTC TGCGCGGGCG GTGTCGCGCT CCCACCAGCG AGCCGAGCAC 
ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG AAGCGGCGTC TGGGCCGCGA GGCCAGCATC 
ATCACAGCCA TCCCTGTGGC TCAGGAGGCC TGCTACGAAC CCACATGCAG CCCTCCACCC 
GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG AACCTGGCAT CACGACGTCT GTCTGTCAGC 
CCATCCTGCG CCTCCAGCAA CTCCCACAGG AACTACTCTT TCCGCCGGGG CTCTGTATGG 
TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT GAAGAAAACA CAACAGAACA CACACCCACT 
CACCACATGC TACAGCCACC TCAAGCTGTC TTCCCAGCAT GCATCTGTGC TGCCGTCCTA 
CCAATAGTGC ACCTCATGGA AGACGGTGAA GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT 
GTTGCCCAGC AAATCCTCTG GAACTGCCTC ATTGAAGATC CAGCTCTCGT TCTCCGCCAC 
TTCCTGGAAA AGCTGACAGT TAGCAACAGA CAGGACGAGC TGATGTACAT GCTTAGGAAG 
CTCCTGCTCA ACATTGGAGA TCTGCCAGCC CAGACCTCTC ACATCCTTTT TAACTACCTG 
GTGGGCCTGA TCATGTATTT TGTGCGGACT CCCTGTGAAT GGGGCATGGA CGCCATCTCG 
GCCACGCTGA CCTTCCTCTG GGAGGTGGTC GGCTACGTGG AGGGGCTCTT CTTTAAAGAC 
CTGAAGCAGA CCATGAAGAA AGAGCAGTGT GAGGTCAAAC TGCTGGTCAC AGCATCGATG 
CCAGGAACCA AAACGCTGGT GGTGCACGGG CAGAATGAAT GTGACATCCC AACCCAGCTA 
CCAGTCCACG AAGACACTCA GTTTGAAGCC CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC 
ATTCCCGAGG CCAGATCGGC ACACTACTTC CTCATGGATA AACGATGGAA CCTCATCCAT 
TATTCAAAGA CATATGTTAG AGACATCTAC CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC 
AACCTGGTCC ACATGTTGCC TGAGAAAGGA CAGGAGCTCA TCCAGAAACA GGTGTTTTCC 
CGTAAACTAG AAGAAGTCGG TCGAGTCCTC TTCCTCATCT CCCTCACACA ACACATGCCA 
GCTGTGCACA AGCAGTCCCA CGTCTCCCTG CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC 
TTCCCCCGCA CCGCCATCGA TGCAGAGTTC TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG 
CTGTTTGGCT TGGACACTCT CCACAAGGTG TTGTGGATCA AACTGTTGGA GGAAATGTTT 
CTGGGCATGC CCAGTGAGTA CCCGTGGGGC GATGAGATGA TGCTGTTCCT TAACGTCTTC 
AACGGGGCCC TTCTGCTGCA CCCCGAGGAC AGCTCCCTGC TGAGGCAGTA CACGGCGACC 
GCCATCAACA CCGCCGTTCA CTTTAACCAT CTGTTCTCCC TGAGCGGGTA CCAGTGGATG 
CTGCCAACCA TGCTGCAGGT CTATGCAGAC TACGAGAGCA ACCCTCTTCT GAGGCAGGGC 
ATTGAGTTTT GCTGCCGGCA GTTTTACATC CTCCATCGAA AACCCTTTAT CCTGCAGCTG 
TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC ACAACCAGCA CCAGTACCGG TCTTTCTAAA 
GGAGTTTCGG CCCAGTGTCT GTTCGACCTG CTGGTCTCTC TAGAAGGTGA AACCCAGGAT 
GCCCTGGATG CTCTGGAGCT GGTAAAAGCT GAGAAGCCTC TACGCTCTCT GGATTTCTGT 
TATGGTAATG AGGACCTGAC CTTTTCTATC AGCGAGGCCA TCAAGCTGTG CGTCACTGTG 
GTTGCATATG CCCCCGAATC CTTCAGGAGT CTCCAGATGC TGATGGTGCT GGAGGCCTTG 
GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC AACACAGTTA CGATGGAGTC TGCTTCGGCT 
GCCAGAGATG AGATTGCGGC CATCGCTGCC CTAGCTACGT CTCTGCAGGC CCTGCTCTAC 
AGCTCTGAAA CCCTCACAAG GCCCATGACG GCTCCCCAGA TATCTCGCTG TGATCAGGGC 
CACAAGGGCG GCACTACAGC TAACCACGCC ATGTCCGGAG GGGTCAACAC CAGGGACAAT 
CTTCACCTGC TGGAGGAGGG CCAGGGCATG CCAAGGGAAG AGCTGGACGA GCGCATTGCC 
AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC CTGTTGAACA TCTGCACCGA GTTTTACAAA 
CACTGCGGGC CACGGCTCAA AATCCTGCAG AACGTTGCAG GCGAGCCGCG GGTCACAGCT 
CTGGAGCTAC TGGACATCAA GTCCCACATG AGGCTGGCAG AGATCGCCCA CGCCCTGCTG 
AAGCTGGCGC CCTACGACAC CCTGACCATG GAGAGCCGAG GGCTGCGGCG TTACATCATG 
GAGATGCTGC CCATCACCGA CTGGTCGTCC GAGGCCGTCC GGCCTGCCCT CATCCTCATC 
CTCAAGAGGC TGGACCGCAT GTTCAACAAG ATCCACAAGA TGCCCACGCT CAGGTGCGCT 
CGCCCTCAGG CTTTATGCAG CAGGAGACAG GTGGAGTGGG AGGCGGCCAG TAGCCTGATC 
GAGGGGATCT GCCTGACCTT GCAGCGACAG CCGATCATCT CGTTCCTGCC TCACCTGCGC 
TCGCTTATCA ACGTCTGCGT CAACCTGGTG ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG 
GACGGGCTGC CACTCCTCCA CCTCAGCCCT TACCTGTCTC CGCCTCTGCC CTTTAGCACC 
GCCGTGGTCC GATTGGTTGC TCTGCAGATT CAGGCCTTGA AGGAAGACTT TCCCCTCAGT 
CACGTGATCT CACCTTTCAC CAATCAGGAA AGGCGGGAGG GGATGCTGCT CAACCTGCTC 
ATTCCCTTCG TGTTAACTGT AGGCTCCGGG AGCAAAGACA GCCCCCACCT GGAGCAGCCG 
GAGGTCTTCC TGCTCCTACA GACAGTCATC AACATCCTGC TTCCCCCTCG TATCATCTCC 
ACATCCCGCA CCAAGAACTT TATGCTTGAC GCCTCTCCGG CTCACTGCTC CACGCCGGGT 
GACCCTGGGA AAGATCTGCG TAGGGAGGGA TTAGCAGAGT CCACGAGCCA GGCTGCTTAT 
TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC GAGCGCTCGC TGGGGAACCA GTGGTATCGG 
CTGAGCCTGC AGGTGAAGGA GATGGCTCTG AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC 
TTCATCGACT TTATCGTCCG AACCCGGATC CCTATTTTTG TCCTGCTCAG ACCCTTTATA 
CAGTGCAAGT TGCTGACCCA GCCGGCCGAT TCCCAGGAAG AGATCACAGC GCGCCACCAC 
ATCGCTGATC AGCTGGAGCG GCGCTTCATC CCACGACCTC TCTGCAAGAG CTCACTGTTT 
GCAGAGTTCA ACAATGAGCT CAAGATCCTC AAAGAGGCTG TGCACAGCGG CTCAGCATAT 
CAGGGCAAGA CATCCATCAG CACGGTGGGC ACCTCCACGT CAGCCTACCG CCTCAGTTTG 
GCCACAATGT CGCGATCCAA CACAGGCACT GGAACAGTTT GGGAGCAGGA CAGCCAACCG 
TCACGGCAGC CCTCACAGGA CACGCTCAGC CGTACCGACG AGGACGATGA AGAGATTGAC 
TCCATGAGCA TTCCCAGCGT GGTGAGCGAG CACGAAGCCT TCATGCCCAG GGTGATGTCA 
CAGCGCCGTT TCTCCAGCCA CGCCACTGGC TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC 
ACCACCATGT TGCCCAGCCA CAGTGAACCC AATGTGCTAG ATGAGTCCCA GGGTCTTCTG 
CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT GTGCAGAGCG AACCAGGTCA GAACCTCCTG 
ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA CTCAGACAGA TGCGACGCCC CCTGCTGTCC 
ATTCCAAAAA CCGAACCACG CGGTCGATCT GGAGCGAGGC TTTCTACAAC GCGCAGGAGC 
ATCCAGCCCA AGAACAAACC ACTGGAAACT TTATTGGACT GTGAAGCGCA TGGAGACCAA 
AAGAGGTCAG TCACCTTTAC AGAGAATCAA GGCCAGCAGG CATCCACGGG GGCAAGCAAG 
CCTCCAACTC CCAGCACTGT GGACCCACCG GGCGAAGGCA GAGGGACGAG TCCTGCCCCA 
TCAAAGTCCC CAACCCTGGA AGTACCCCCC ACCTCTTCAG CCATGATCTT GGCTGACCTG 
CACGGACCTA CAAATGCACA GGTCCCTCAA GCATTAAGCA GCAAGGAGAA GAGAGAGCAG 
TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC CCATCCATCT CCCGGCCTTC CACTCCAACA 
CCCCCATCCC GAACCTGCTC CCCTCTCCCC CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG 
TCCCGCACCT CCTCTCCTCT TCCACCGCCA CTCCCAGTGC TGAGCACAGC ACCCGCTCCG 
GGTCCACCGC CGCCACCTCC ATTACCACCA ACTCATCTTG CTGCTGCTCC TCCTCCTCCA 
CCACCCCCGG GGCCTTCTAG GATCAGAGAG AGCCCCGGGG ACGAGGACAC AACCGCCCTG 
CTGCCGCGCA GCGACACCCT GCTGCACCTC AGCGAGGACG ATGGCACCGA GAACCCGCTT 
CTCACCCCGC TGCTGTCCCC TCCTCCCCCC CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC 
CTCTCACCCG TCGACCTGGA CCTGGATGAG TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009237.2
CDS	397..10818
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_023153469.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153469.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGACA AGGCGGGCTT CGGAAACAAC 
TTCGCCACAG GAGACAACAA ATCTGTAGCC CAGAATATGG AGGCCGTGGT GGTGGGCTGC 
ATGTTCAAGT CCCTCATCAC CCGCTGCGCC TCAACCACAC ATGAGCTGCA CAGCCCCGAG 
AACCTGGGTC TGTACTGTGA CATCCGCCAG CTGGTCCAGT TCATTAAAGA GGCCCACGGA 
AACGTGTTCC GCAGGGTGGC ACTGAGCGCG CTCCTGGACA GCGCGGAGAA GGTCACCGCC 
ACCAAGAAAC CTGATGAGAA GGAGGAGACC AAACAGCCCG GTCCCAGGAG GTCAGAGGCG 
GGTGTGTCCG GGGAGAAGGG TCAGGTGTCC AGTACTGCAG ACGAGTGTCG CAGCTACATC 
TCCAGTAGAC CCCCCCAGAC ACCCGAACAT GAAGAACAGA TCCCGGGTGC TCTGCTGGGC 
AGGAAGGATT TTTGGAGGAA GATGTTCAAG TCGCAGAGCG CCGCCAGTGA CACCAGCAGC 
CAGTCGGAAC AAGACACCTC AGAGTGCACC ACCGCACACT CTGGCACCAC CACCGACCGC 
CGCTCACGAT CCAGATCCCG TCGCATTTCG CTTCGCAAGA AGTTGAAGCT GCCTATCGGG 
AACTGGTTGA AGCGCTCCTC TCTCTCTGGA CTGACTGACG GCGTCGAGGA CTTGCTGGAT 
ATCAGCTCGG TGGATCGACT CTCCTTCATA CGTCAGAGTT CAAAGGTGAA GTTCACTAGT 
GCAGTGAAGC TATCTGAAGG TGGCGCTGTA GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG 
AATTTCTTCA AGCGGCTCGG TAAATGGAGG TCTGGCAGGA GGAATCCATC CAAGCTTCAC 
CACACAGAAG AGAAAGATGG ACCTCACGGT TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG 
GCCATGAAGA ACAAGAATGT AGTGAATCTG GGCGCCATCC GACAGGGTAT GAAACGCTTC 
CAGTTTCTCC TGAACTGCTG CGAGCCAGGA ACCATACCAG ATGCCTCCAT CCTCGCCGCT 
GCTCTGGACC TCGAAGCCCC AGTTGTGGCC AGGGCCTCCC TTTTCTTGGA ATGTGCCAGA 
TTTGTCCACC GGTGTAATCG TGGCAACTGG CCCGAATGGA TGAAGGGCCA CCACGTAAAC 
ATCACCAAGA GAGGGCTGTC CAGGGGCCGA TCGCCTATTG TGGGCAATAA AAGAAACCAG 
AAGCTCCAGT GGAACGCTGC CAAGCATTTC TGCCAGTGGG GAGACGCGAT TGGCACGAGG 
CTCAGCGAAC TCTGCCACTC GGACAGTGAG AGCCCCGCCA ACATCCTGGG TTATATTTAC 
GATGAAGAGA CCAAACGCAG GATGAGGAAA GAAGACGAGG AAGAAGACTA TTTAGATGAC 
AACACGGTCA ATCCTACAAA ATGTGGCTGC CCCTTCGCAC TGAAGATGGC CGCTTGCCAG 
CTGTTGTTGG AGATCACCAC TTTCCTGCGA GAGACCTTCC CCTGCTTACC ACGACCGCGC 
ACCGAACCGC TTGTGGATCT GGACAGCTGC CGCCTGCGTC TGGACCCAGA GCTCGGCCGC 
CATCGCTACG AGAGGAAGAT CAGCTTTGCG GGCATCCTCG ACGACGAAGA CGGGCACGAT 
TCCCTCAACA GCAGCAGCCA CACTCTGAAG TCTGACACCA CCTGTGATGA TAAGAAGACC 
CAGGAGGCTC AGGACAATCT CTCCCCACTC CCCTGCTCTC CAGCACCAGT GCGGAAGATT 
CGTATCGGAG GCTCGCGCCT GCTTCAGATC AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG 
GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG GGGAGTCTGA AGAGCACCAA GCTGTCTCGG 
CAGGACTCTG AGTCTGAGAA CGAAGAAGGC CTGCTGTCAC AAACGCAGAG CAGGGACACA 
GTTACTGACA TCGGCAGTCC CTTCAGCACC AGTGAACCCA GCATCGAGCC AGAAGGTCAG 
AGCTCAGGAG GGACGGAGGA CAACTACCAC CGCAACATGT CCTGGCTTCA CATTATGATC 
CTGCTGTGCA ACCAGCAGAG TTTCATCTGT ACCCACATAG ACTTCTGCCA CCCACGCTGC 
TACCAACACC ACAGCCGCTC CTGCGCCCGT CTGGTCCGTG CCATCAAGCT CGTGTACGGG 
GAGACGGTGG ACAGCCTGAG GGAGGACAGC GCCTCCTCTG GTAACATCGG AGGACGTGTG 
AAGAAAAACA AAGAGTGTTC AGACAAGTCT TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA 
CCCACTGACT CCAACACAGA AGGGAAGAAG GATACAGGCA TGCTCAAGTA CATCCGCAAT 
CAGGTGATGA GTTTGTCACC AGCTCCTCTT TCACTGCTGA TCAAAGCAGC TCCTATCTTG 
ACTGACGACA TGTACGGAGA CATCCAGCCC GCAGCCTGGG AACTCCTACT CAGTGTGGAT 
GAACATATGG CAGCCGCTGC TGCTGCCTTG TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT 
GCAGTGACCG AAATGATGAT GGCAGAGTTC CAGCACCCTG AGGCCTGCCA GCGCATTAAC 
TCCATCCTGA AGTTTTACAC TCTGTGGCGT TTTCGCTACC AAGTGTGGCC TCGCATGGAG 
GAAGGAGCCC AGCAGATCTT TAAGATCCCT CCACCCAGCA TTAACTTCAC TCTCCCTTCT 
CCAATACTGG GCATGCCCTG TGTCCCCATA TTTGACCCTC CCTGGGTGCC CCAGAACACT 
GGCAGCGTCC AGGATCCAAT CAACGAAGAC CAGTCTTACC CCACATTAAT TCAGTCAGTC 
CATGCCATAA AATCCTTCTC TGCGCGGGCG GTGTCGCGCT CCCACCAGCG AGCCGAGCAC 
ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG AAGCGGCGTC TGGGCCGCGA GGCCAGCATC 
ATCACAGCCA TCCCTGTGGC TCAGGAGGCC TGCTACGAAC CCACATGCAG CCCTCCACCC 
GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG AACCTGGCAT CACGACGTCT GTCTGTCAGC 
CCATCCTGCG CCTCCAGCAA CTCCCACAGG AACTACTCTT TCCGCCGGGG CTCTGTATGG 
TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT GAAGAAAACA CAACAGAACA CACACCCACT 
CACCACATGC TACAGCCACC TCAAGCTGTC TTCCCAGCAT GCATCTGTGC TGCCGTCCTA 
CCAATAGTGC ACCTCATGGA AGACGGTGAA GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT 
GTTGCCCAGC AAATCCTCTG GAACTGCCTC ATTGAAGATC CAGCTCTCGT TCTCCGCCAC 
TTCCTGGAAA AGCTGACAGT TAGCAACAGA CAGGACGAGC TGATGTACAT GCTTAGGAAG 
CTCCTGCTCA ACATTGGAGA TCTGCCAGCC CAGACCTCTC ACATCCTTTT TAACTACCTG 
GTGGGCCTGA TCATGTATTT TGTGCGGACT CCCTGTGAAT GGGGCATGGA CGCCATCTCG 
GCCACGCTGA CCTTCCTCTG GGAGGTGGTC GGCTACGTGG AGGGGCTCTT CTTTAAAGAC 
CTGAAGCAGA CCATGAAGAA AGAGCAGTGT GAGGTCAAAC TGCTGGTCAC AGCATCGATG 
CCAGGAACCA AAACGCTGGT GGTGCACGGG CAGAATGAAT GTGACATCCC AACCCAGCTA 
CCAGTCCACG AAGACACTCA GTTTGAAGCC CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC 
ATTCCCGAGG CCAGATCGGC ACACTACTTC CTCATGGATA AACGATGGAA CCTCATCCAT 
TATTCAAAGA CATATGTTAG AGACATCTAC CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC 
AACCTGGTCC ACATGTTGCC TGAGAAAGGA CAGGAGCTCA TCCAGAAACA GGTGTTTTCC 
CGTAAACTAG AAGAAGTCGG TCGAGTCCTC TTCCTCATCT CCCTCACACA ACACATGCCA 
GCTGTGCACA AGCAGTCCCA CGTCTCCCTG CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC 
TTCCCCCGCA CCGCCATCGA TGCAGAGTTC TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG 
CTGTTTGGCT TGGACACTCT CCACAAGGTG TTGTGGATCA AACTGTTGGA GGAAATGTTT 
CTGGGCATGC CCAGTGAGTA CCCGTGGGGC GATGAGATGA TGCTGTTCCT TAACGTCTTC 
AACGGGGCCC TTCTGCTGCA CCCCGAGGAC AGCTCCCTGC TGAGGCAGTA CACGGCGACC 
GCCATCAACA CCGCCGTTCA CTTTAACCAT CTGTTCTCCC TGAGCGGGTA CCAGTGGATG 
CTGCCAACCA TGCTGCAGGT CTATGCAGAC TACGAGAGCA ACCCTCTTCT GAGGCAGGGC 
ATTGAGTTTT GCTGCCGGCA GTTTTACATC CTCCATCGAA AACCCTTTAT CCTGCAGCTG 
TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC ACAACCAGCA CCAGTACCGG TCTTTCTAAA 
GGAGTTTCGG CCCAGTGTCT GTTCGACCTG CTGGTCTCTC TAGAAGGTGA AACCCAGGAT 
GCCCTGGATG CTCTGGAGCT GGTAAAAGCT GAGAAGCCTC TACGCTCTCT GGATTTCTGT 
TATGGTAATG AGGACCTGAC CTTTTCTATC AGCGAGGCCA TCAAGCTGTG CGTCACTGTG 
GTTGCATATG CCCCCGAATC CTTCAGGAGT CTCCAGATGC TGATGGTGCT GGAGGCCTTG 
GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC AACACAGTTA CGATGGAGTC TGCTTCGGCT 
GCCAGAGATG AGATTGCGGC CATCGCTGCC CTAGCTACGT CTCTGCAGGC CCTGCTCTAC 
AGCTCTGAAA CCCTCACAAG GCCCATGACG GCTCCCCAGA TATCTCGCTG TGATCAGGGC 
CACAAGGGCG GCACTACAGC TAACCACGCC ATGTCCGGAG GGGTCAACAC CAGGGACAAT 
CTTCACCTGC TGGAGGAGGG CCAGGGCATG CCAAGGGAAG AGCTGGACGA GCGCATTGCC 
AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC CTGTTGAACA TCTGCACCGA GTTTTACAAA 
CACTGCGGGC CACGGCTCAA AATCCTGCAG AACGTTGCAG GCGAGCCGCG GGTCACAGCT 
CTGGAGCTAC TGGACATCAA GTCCCACATG AGGCTGGCAG AGATCGCCCA CGCCCTGCTG 
AAGCTGGCGC CCTACGACAC CCTGACCATG GAGAGCCGAG GGCTGCGGCG TTACATCATG 
GAGATGCTGC CCATCACCGA CTGGTCGTCC GAGGCCGTCC GGCCTGCCCT CATCCTCATC 
CTCAAGAGGC TGGACCGCAT GTTCAACAAG ATCCACAAGA TGCCCACGCT CAGGTGCGCT 
CGCCCTCAGG CTTTATGCAG CAGGAGACAG GTGGAGTGGG AGGCGGCCAG TAGCCTGATC 
GAGGGGATCT GCCTGACCTT GCAGCGACAG CCGATCATCT CGTTCCTGCC TCACCTGCGC 
TCGCTTATCA ACGTCTGCGT CAACCTGGTG ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG 
GACGGGCTGC CACTCCTCCA CCTCAGCCCT TACCTGTCTC CGCCTCTGCC CTTTAGCACC 
GCCGTGGTCC GATTGGTTGC TCTGCAGATT CAGGCCTTGA AGGAAGACTT TCCCCTCAGT 
CACGTGATCT CACCTTTCAC CAATCAGGAA AGGCGGGAGG GGATGCTGCT CAACCTGCTC 
ATTCCCTTCG TGTTAACTGT AGGCTCCGGG AGCAAAGACA GCCCCCACCT GGAGCAGCCG 
GAGGTCTTCC TGCTCCTACA GACAGTCATC AACATCCTGC TTCCCCCTCG TATCATCTCC 
ACATCCCGCA CCAAGAACTT TATGCTTGAC GCCTCTCCGG CTCACTGCTC CACGCCGGGT 
GACCCTGGGA AAGATCTGCG TAGGGAGGGA TTAGCAGAGT CCACGAGCCA GGCTGCTTAT 
TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC GAGCGCTCGC TGGGGAACCA GTGGTATCGG 
CTGAGCCTGC AGGTGAAGGA GATGGCTCTG AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC 
TTCATCGACT TTATCGTCCG AACCCGGATC CCTATTTTTG TCCTGCTCAG ACCCTTTATA 
CAGTGCAAGT TGCTGACCCA GCCGGCCGAT TCCCAGGAAG AGATCACAGC GCGCCACCAC 
ATCGCTGATC AGCTGGAGCG GCGCTTCATC CCACGACCTC TCTGCAAGAG CTCACTGTTT 
GCAGAGTTCA ACAATGAGCT CAAGATCCTC AAAGAGGCTG TGCACAGCGG CTCAGCATAT 
CAGGGCAAGA CATCCATCAG CACGGTGGGC ACCTCCACGT CAGCCTACCG CCTCAGTTTG 
GCCACAATGT CGCGATCCAA CACAGGCACT GGAACAGTTT GGGAGCAGGA CAGCCAACCG 
TCACGGCAGC CCTCACAGGA CACGCTCAGC CGTACCGACG AGGACGATGA AGAGATTGAC 
TCCATGAGCA TTCCCAGCGT GGTGAGCGAG CACGAAGCCT TCATGCCCAG GGTGATGTCA 
CAGCGCCGTT TCTCCAGCCA CGCCACTGGC TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC 
ACCACCATGT TGCCCAGCCA CAGTGAACCC AATGTGCTAG ATGAGTCCCA GGGTCTTCTG 
CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT GTGCAGAGCG AACCAGGTCA GAACCTCCTG 
ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA CTCAGACAGA TGCGACGCCC CCTGCTGTCC 
ATTCCAAAAA CCGAACCACG CGGTCGATCT GGAGCGAGGC TTTCTACAAC GCGCAGGAGC 
ATCCAGCCCA AGAACAAACC ACTGGAAACT TTATTGGACT GTGAAGCGCA TGGAGACCAA 
AAGAGGTCAG TCACCTTTAC AGAGAATCAA GGCCAGCAGG CATCCACGGG GGCAAGCAAG 
CCTCCAACTC CCAGCACTGT GGACCCACCG GGCGAAGGCA GAGGGACGAG TCCTGCCCCA 
TCAAAGTCCC CAACCCTGGA AGTACCCCCC ACCTCTTCAG CCATGATCTT GGCTGACCTG 
CACGGACCTA CAAATGCACA GGTCCCTCAA GCATTAAGCA GCAAGGAGAA GAGAGAGCAG 
TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC CCATCCATCT CCCGGCCTTC CACTCCAACA 
CCCCCATCCC GAACCTGCTC CCCTCTCCCC CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG 
TCCCGCACCT CCTCTCCTCT TCCACCGCCA CTCCCAGTGC TGAGCACAGC ACCCGCTCCG 
GGTCCACCGC CGCCACCTCC ATTACCACCA ACTCATCTTG CTGCTGCTCC TCCTCCTCCA 
CCACCCCCGG GGCCTTCTAG GATCAGAGAG AGCCCCGGGG ACGAGGACAC AACCGCCCTG 
CTGCCGCGCA GCGACACCCT GCTGCACCTC AGCGAGGACG ATGGCACCGA GAACCCGCTT 
CTCACCCCGC TGCTGTCCCC TCCTCCCCCC CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC 
CTCTCACCCG TCGACCTGGA CCTGGATGAG TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_023153470.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023009238.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.

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CloneID	OMa370058
Clone ID Related Accession (Same CDS sequence)	XM_023153470.2
Accession Version	XM_023153470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023153470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGT 
GAAGAACAGA TCCCGGGTGC TCTGCTGGGC AGGAAGGATT TTTGGAGGAA GATGTTCAAG 
TCGCAGAGCG CCGCCAGTGA CACCAGCAGC CAGTCGGAAC AAGACACCTC AGAGTGCACC 
ACCGCACACT CTGGCACCAC CACCGACCGC CGCTCACGAT CCAGATCCCG TCGCATTTCG 
CTTCGCAAGA AGTTGAAGCT GCCTATCGGG AACTGGTTGA AGCGCTCCTC TCTCTCTGGA 
CTGACTGACG GCGTCGAGGA CTTGCTGGAT ATCAGCTCGG TGGATCGACT CTCCTTCATA 
CGTCAGAGTT CAAAGGTGAA GTTCACTAGT GCAGTGAAGC TATCTGAAGG TGGCGCTGTA 
GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG AATTTCTTCA AGCGGCTCGG TAAATGGAGG 
TCTGGCAGGA GGAATCCATC CAAGCTTCAC CACACAGAAG AGAAAGATGG ACCTCACGGT 
TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG GCCATGAAGA ACAAGAATGT AGTGAATCTG 
GGCGCCATCC GACAGGGTAT GAAACGCTTC CAGTTTCTCC TGAACTGCTG CGAGCCAGGA 
ACCATACCAG ATGCCTCCAT CCTCGCCGCT GCTCTGGACC TCGAAGCCCC AGTTGTGGCC 
AGGGCCTCCC TTTTCTTGGA ATGTGCCAGA TTTGTCCACC GGTGTAATCG TGGCAACTGG 
CCCGAATGGA TGAAGGGCCA CCACGTAAAC ATCACCAAGA GAGGGCTGTC CAGGGGCCGA 
TCGCCTATTG TGGGCAATAA AAGAAACCAG AAGCTCCAGT GGAACGCTGC CAAGCATTTC 
TGCCAGTGGG GAGACGCGAT TGGCACGAGG CTCAGCGAAC TCTGCCACTC GGACAGTGAG 
AGCCCCGCCA ACATCCTGGG TTATATTTAC GATGAAGAGA CCAAACGCAG GATGAGGAAA 
GAAGACGAGG AAGAAGACTA TTTAGATGAC AACACGGTCA ATCCTACAAA ATGTGGCTGC 
CCCTTCGCAC TGAAGATGGC CGCTTGCCAG CTGTTGTTGG AGATCACCAC TTTCCTGCGA 
GAGACCTTCC CCTGCTTACC ACGACCGCGC ACCGAACCGC TTGTGGATCT GGACAGCTGC 
CGCCTGCGTC TGGACCCAGA GCTCGGCCGC CATCGCTACG AGAGGAAGAT CAGCTTTGCG 
GGCATCCTCG ACGACGAAGA CGGGCACGAT TCCCTCAACA GCAGCAGCCA CACTCTGAAG 
TCTGACACCA CCTGTGATGA TAAGAAGACC CAGGAGGCTC AGGACAATCT CTCCCCACTC 
CCCTGCTCTC CAGCACCAGT GCGGAAGATT CGTATCGGAG GCTCGCGCCT GCTTCAGATC 
AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG 
GGGAGTCTGA AGAGCACCAA GCTGTCTCGG CAGGACTCTG AGTCTGAGAA CGAAGAAGGC 
CTGCTGTCAC AAACGCAGAG CAGGGACACA GTTACTGACA TCGGCAGTCC CTTCAGCACC 
AGTGAACCCA GCATCGAGCC AGAAGGTCAG AGCTCAGGAG GGACGGAGGA CAACTACCAC 
CGCAACATGT CCTGGCTTCA CATTATGATC CTGCTGTGCA ACCAGCAGAG TTTCATCTGT 
ACCCACATAG ACTTCTGCCA CCCACGCTGC TACCAACACC ACAGCCGCTC CTGCGCCCGT 
CTGGTCCGTG CCATCAAGCT CGTGTACGGG GAGACGGTGG ACAGCCTGAG GGAGGACAGC 
GCCTCCTCTG GTAACATCGG AGGACGTGTG AAGAAAAACA AAGAGTGTTC AGACAAGTCT 
TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA CCCACTGACT CCAACACAGA AGGGAAGAAG 
GATACAGGCA TGCTCAAGTA CATCCGCAAT CAGGTGATGA GTTTGTCACC AGCTCCTCTT 
TCACTGCTGA TCAAAGCAGC TCCTATCTTG ACTGACGACA TGTACGGAGA CATCCAGCCC 
GCAGCCTGGG AACTCCTACT CAGTGTGGAT GAACATATGG CAGCCGCTGC TGCTGCCTTG 
TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT GCAGTGACCG AAATGATGAT GGCAGAGTTC 
CAGCACCCTG AGGCCTGCCA GCGCATTAAC TCCATCCTGA AGTTTTACAC TCTGTGGCGT 
TTTCGCTACC AAGTGTGGCC TCGCATGGAG GAAGGAGCCC AGCAGATCTT TAAGATCCCT 
CCACCCAGCA TTAACTTCAC TCTCCCTTCT CCAATACTGG GCATGCCCTG TGTCCCCATA 
TTTGACCCTC CCTGGGTGCC CCAGAACACT GGCAGCGTCC AGGATCCAAT CAACGAAGAC 
CAGTCTTACC CCACATTAAT TCAGTCAGTC CATGCCATAA AATCCTTCTC TGCGCGGGCG 
GTGTCGCGCT CCCACCAGCG AGCCGAGCAC ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG 
AAGCGGCGTC TGGGCCGCGA GGCCAGCATC ATCACAGCCA TCCCTGTGGC TCAGGAGGCC 
TGCTACGAAC CCACATGCAG CCCTCCACCC GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG 
AACCTGGCAT CACGACGTCT GTCTGTCAGC CCATCCTGCG CCTCCAGCAA CTCCCACAGG 
AACTACTCTT TCCGCCGGGG CTCTGTATGG TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT 
GAAGAAAACA CAACAGAACA CACACCCACT CACCACATGC TACAGCCACC TCAAGCTGTC 
TTCCCAGCAT GCATCTGTGC TGCCGTCCTA CCAATAGTGC ACCTCATGGA AGACGGTGAA 
GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT GTTGCCCAGC AAATCCTCTG GAACTGCCTC 
ATTGAAGATC CAGCTCTCGT TCTCCGCCAC TTCCTGGAAA AGCTGACAGT TAGCAACAGA 
CAGGACGAGC TGATGTACAT GCTTAGGAAG CTCCTGCTCA ACATTGGAGA TCTGCCAGCC 
CAGACCTCTC ACATCCTTTT TAACTACCTG GTGGGCCTGA TCATGTATTT TGTGCGGACT 
CCCTGTGAAT GGGGCATGGA CGCCATCTCG GCCACGCTGA CCTTCCTCTG GGAGGTGGTC 
GGCTACGTGG AGGGGCTCTT CTTTAAAGAC CTGAAGCAGA CCATGAAGAA AGAGCAGTGT 
GAGGTCAAAC TGCTGGTCAC AGCATCGATG CCAGGAACCA AAACGCTGGT GGTGCACGGG 
CAGAATGAAT GTGACATCCC AACCCAGCTA CCAGTCCACG AAGACACTCA GTTTGAAGCC 
CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC ATTCCCGAGG CCAGATCGGC ACACTACTTC 
CTCATGGATA AACGATGGAA CCTCATCCAT TATTCAAAGA CATATGTTAG AGACATCTAC 
CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC AACCTGGTCC ACATGTTGCC TGAGAAAGGA 
CAGGAGCTCA TCCAGAAACA GGTGTTTTCC CGTAAACTAG AAGAAGTCGG TCGAGTCCTC 
TTCCTCATCT CCCTCACACA ACACATGCCA GCTGTGCACA AGCAGTCCCA CGTCTCCCTG 
CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC TTCCCCCGCA CCGCCATCGA TGCAGAGTTC 
TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG CTGTTTGGCT TGGACACTCT CCACAAGGTG 
TTGTGGATCA AACTGTTGGA GGAAATGTTT CTGGGCATGC CCAGTGAGTA CCCGTGGGGC 
GATGAGATGA TGCTGTTCCT TAACGTCTTC AACGGGGCCC TTCTGCTGCA CCCCGAGGAC 
AGCTCCCTGC TGAGGCAGTA CACGGCGACC GCCATCAACA CCGCCGTTCA CTTTAACCAT 
CTGTTCTCCC TGAGCGGGTA CCAGTGGATG CTGCCAACCA TGCTGCAGGT CTATGCAGAC 
TACGAGAGCA ACCCTCTTCT GAGGCAGGGC ATTGAGTTTT GCTGCCGGCA GTTTTACATC 
CTCCATCGAA AACCCTTTAT CCTGCAGCTG TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC 
ACAACCAGCA CCAGTACCGG TCTTTCTAAA GGAGTTTCGG CCCAGTGTCT GTTCGACCTG 
CTGGTCTCTC TAGAAGGTGA AACCCAGGAT GCCCTGGATG CTCTGGAGCT GGTAAAAGCT 
GAGAAGCCTC TACGCTCTCT GGATTTCTGT TATGGTAATG AGGACCTGAC CTTTTCTATC 
AGCGAGGCCA TCAAGCTGTG CGTCACTGTG GTTGCATATG CCCCCGAATC CTTCAGGAGT 
CTCCAGATGC TGATGGTGCT GGAGGCCTTG GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC 
AACACAGTTA CGATGGAGTC TGCTTCGGCT GCCAGAGATG AGATTGCGGC CATCGCTGCC 
CTAGCTACGT CTCTGCAGGC CCTGCTCTAC AGCTCTGAAA CCCTCACAAG GCCCATGACG 
GCTCCCCAGA TATCTCGCTG TGATCAGGGC CACAAGGGCG GCACTACAGC TAACCACGCC 
ATGTCCGGAG GGGTCAACAC CAGGGACAAT CTTCACCTGC TGGAGGAGGG CCAGGGCATG 
CCAAGGGAAG AGCTGGACGA GCGCATTGCC AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC 
CTGTTGAACA TCTGCACCGA GTTTTACAAA CACTGCGGGC CACGGCTCAA AATCCTGCAG 
AACGTTGCAG GCGAGCCGCG GGTCACAGCT CTGGAGCTAC TGGACATCAA GTCCCACATG 
AGGCTGGCAG AGATCGCCCA CGCCCTGCTG AAGCTGGCGC CCTACGACAC CCTGACCATG 
GAGAGCCGAG GGCTGCGGCG TTACATCATG GAGATGCTGC CCATCACCGA CTGGTCGTCC 
GAGGCCGTCC GGCCTGCCCT CATCCTCATC CTCAAGAGGC TGGACCGCAT GTTCAACAAG 
ATCCACAAGA TGCCCACGCT CAGGTGCGCT CGCCCTCAGG CTTTATGCAG CAGGAGACAG 
GTGGAGTGGG AGGCGGCCAG TAGCCTGATC GAGGGGATCT GCCTGACCTT GCAGCGACAG 
CCGATCATCT CGTTCCTGCC TCACCTGCGC TCGCTTATCA ACGTCTGCGT CAACCTGGTG 
ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG GACGGGCTGC CACTCCTCCA CCTCAGCCCT 
TACCTGTCTC CGCCTCTGCC CTTTAGCACC GCCGTGGTCC GATTGGTTGC TCTGCAGATT 
CAGGCCTTGA AGGAAGACTT TCCCCTCAGT CACGTGATCT CACCTTTCAC CAATCAGGAA 
AGGCGGGAGG GGATGCTGCT CAACCTGCTC ATTCCCTTCG TGTTAACTGT AGGCTCCGGG 
AGCAAAGACA GCCCCCACCT GGAGCAGCCG GAGGTCTTCC TGCTCCTACA GACAGTCATC 
AACATCCTGC TTCCCCCTCG TATCATCTCC ACATCCCGCA CCAAGAACTT TATGCTTGAC 
GCCTCTCCGG CTCACTGCTC CACGCCGGGT GACCCTGGGA AAGATCTGCG TAGGGAGGGA 
TTAGCAGAGT CCACGAGCCA GGCTGCTTAT TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC 
GAGCGCTCGC TGGGGAACCA GTGGTATCGG CTGAGCCTGC AGGTGAAGGA GATGGCTCTG 
AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC TTCATCGACT TTATCGTCCG AACCCGGATC 
CCTATTTTTG TCCTGCTCAG ACCCTTTATA CAGTGCAAGT TGCTGACCCA GCCGGCCGAT 
TCCCAGGAAG AGATCACAGC GCGCCACCAC ATCGCTGATC AGCTGGAGCG GCGCTTCATC 
CCACGACCTC TCTGCAAGAG CTCACTGTTT GCAGAGTTCA ACAATGAGCT CAAGATCCTC 
AAAGAGGCTG TGCACAGCGG CTCAGCATAT CAGGGCAAGA CATCCATCAG CACGGTGGGC 
ACCTCCACGT CAGCCTACCG CCTCAGTTTG GCCACAATGT CGCGATCCAA CACAGGCACT 
GGAACAGTTT GGGAGCAGGA CAGCCAACCG TCACGGCAGC CCTCACAGGA CACGCTCAGC 
CGTACCGACG AGGACGATGA AGAGATTGAC TCCATGAGCA TTCCCAGCGT GGTGAGCGAG 
CACGAAGCCT TCATGCCCAG GGTGATGTCA CAGCGCCGTT TCTCCAGCCA CGCCACTGGC 
TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC ACCACCATGT TGCCCAGCCA CAGTGAACCC 
AATGTGCTAG ATGAGTCCCA GGGTCTTCTG CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT 
GTGCAGAGCG AACCAGGTCA GAACCTCCTG ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA 
CTCAGACAGA TGCGACGCCC CCTGCTGTCC ATTCCAAAAA CCGAACCACG CGGTCGATCT 
GGAGCGAGGC TTTCTACAAC GCGCAGGAGC ATCCAGCCCA AGAACAAACC ACTGGAAACT 
TTATTGGACT GTGAAGCGCA TGGAGACCAA AAGAGGTCAG TCACCTTTAC AGAGAATCAA 
GGCCAGCAGG CATCCACGGG GGCAAGCAAG CCTCCAACTC CCAGCACTGT GGACCCACCG 
GGCGAAGGCA GAGGGACGAG TCCTGCCCCA TCAAAGTCCC CAACCCTGGA AGTACCCCCC 
ACCTCTTCAG CCATGATCTT GGCTGACCTG CACGGACCTA CAAATGCACA GGTCCCTCAA 
GCATTAAGCA GCAAGGAGAA GAGAGAGCAG TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC 
CCATCCATCT CCCGGCCTTC CACTCCAACA CCCCCATCCC GAACCTGCTC CCCTCTCCCC 
CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG TCCCGCACCT CCTCTCCTCT TCCACCGCCA 
CTCCCAGTGC TGAGCACAGC ACCCGCTCCG GGTCCACCGC CGCCACCTCC ATTACCACCA 
ACTCATCTTG CTGCTGCTCC TCCTCCTCCA CCACCCCCGG GGCCTTCTAG GATCAGAGAG 
AGCCCCGGGG ACGAGGACAC AACCGCCCTG CTGCCGCGCA GCGACACCCT GCTGCACCTC 
AGCGAGGACG ATGGCACCGA GAACCCGCTT CTCACCCCGC TGCTGTCCCC TCCTCCCCCC 
CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC CTCTCACCCG TCGACCTGGA CCTGGATGAG 
TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023009238.2
CDS	397..10788
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRSEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_023153470.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023153470.2

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGT 
GAAGAACAGA TCCCGGGTGC TCTGCTGGGC AGGAAGGATT TTTGGAGGAA GATGTTCAAG 
TCGCAGAGCG CCGCCAGTGA CACCAGCAGC CAGTCGGAAC AAGACACCTC AGAGTGCACC 
ACCGCACACT CTGGCACCAC CACCGACCGC CGCTCACGAT CCAGATCCCG TCGCATTTCG 
CTTCGCAAGA AGTTGAAGCT GCCTATCGGG AACTGGTTGA AGCGCTCCTC TCTCTCTGGA 
CTGACTGACG GCGTCGAGGA CTTGCTGGAT ATCAGCTCGG TGGATCGACT CTCCTTCATA 
CGTCAGAGTT CAAAGGTGAA GTTCACTAGT GCAGTGAAGC TATCTGAAGG TGGCGCTGTA 
GGGCCGGAGT ACGGCAGGGA CGAGGAGGAG AATTTCTTCA AGCGGCTCGG TAAATGGAGG 
TCTGGCAGGA GGAATCCATC CAAGCTTCAC CACACAGAAG AGAAAGATGG ACCTCACGGT 
TTCCAGGAGC GTCTCGCAGC CAGCCAGGAG GCCATGAAGA ACAAGAATGT AGTGAATCTG 
GGCGCCATCC GACAGGGTAT GAAACGCTTC CAGTTTCTCC TGAACTGCTG CGAGCCAGGA 
ACCATACCAG ATGCCTCCAT CCTCGCCGCT GCTCTGGACC TCGAAGCCCC AGTTGTGGCC 
AGGGCCTCCC TTTTCTTGGA ATGTGCCAGA TTTGTCCACC GGTGTAATCG TGGCAACTGG 
CCCGAATGGA TGAAGGGCCA CCACGTAAAC ATCACCAAGA GAGGGCTGTC CAGGGGCCGA 
TCGCCTATTG TGGGCAATAA AAGAAACCAG AAGCTCCAGT GGAACGCTGC CAAGCATTTC 
TGCCAGTGGG GAGACGCGAT TGGCACGAGG CTCAGCGAAC TCTGCCACTC GGACAGTGAG 
AGCCCCGCCA ACATCCTGGG TTATATTTAC GATGAAGAGA CCAAACGCAG GATGAGGAAA 
GAAGACGAGG AAGAAGACTA TTTAGATGAC AACACGGTCA ATCCTACAAA ATGTGGCTGC 
CCCTTCGCAC TGAAGATGGC CGCTTGCCAG CTGTTGTTGG AGATCACCAC TTTCCTGCGA 
GAGACCTTCC CCTGCTTACC ACGACCGCGC ACCGAACCGC TTGTGGATCT GGACAGCTGC 
CGCCTGCGTC TGGACCCAGA GCTCGGCCGC CATCGCTACG AGAGGAAGAT CAGCTTTGCG 
GGCATCCTCG ACGACGAAGA CGGGCACGAT TCCCTCAACA GCAGCAGCCA CACTCTGAAG 
TCTGACACCA CCTGTGATGA TAAGAAGACC CAGGAGGCTC AGGACAATCT CTCCCCACTC 
CCCTGCTCTC CAGCACCAGT GCGGAAGATT CGTATCGGAG GCTCGCGCCT GCTTCAGATC 
AAAGGAGCCC GCAGTTTTCG TGTGAAGAAG GTTGGCTCGC TGTCTTCGAT ACGCAGAGCG 
GGGAGTCTGA AGAGCACCAA GCTGTCTCGG CAGGACTCTG AGTCTGAGAA CGAAGAAGGC 
CTGCTGTCAC AAACGCAGAG CAGGGACACA GTTACTGACA TCGGCAGTCC CTTCAGCACC 
AGTGAACCCA GCATCGAGCC AGAAGGTCAG AGCTCAGGAG GGACGGAGGA CAACTACCAC 
CGCAACATGT CCTGGCTTCA CATTATGATC CTGCTGTGCA ACCAGCAGAG TTTCATCTGT 
ACCCACATAG ACTTCTGCCA CCCACGCTGC TACCAACACC ACAGCCGCTC CTGCGCCCGT 
CTGGTCCGTG CCATCAAGCT CGTGTACGGG GAGACGGTGG ACAGCCTGAG GGAGGACAGC 
GCCTCCTCTG GTAACATCGG AGGACGTGTG AAGAAAAACA AAGAGTGTTC AGACAAGTCT 
TGTCTGAGGA CCCCGTCCAT GAAGAGAAGA CCCACTGACT CCAACACAGA AGGGAAGAAG 
GATACAGGCA TGCTCAAGTA CATCCGCAAT CAGGTGATGA GTTTGTCACC AGCTCCTCTT 
TCACTGCTGA TCAAAGCAGC TCCTATCTTG ACTGACGACA TGTACGGAGA CATCCAGCCC 
GCAGCCTGGG AACTCCTACT CAGTGTGGAT GAACATATGG CAGCCGCTGC TGCTGCCTTG 
TTTCTCCTGT GCGCCGTAAA GGTCCCTGAT GCAGTGACCG AAATGATGAT GGCAGAGTTC 
CAGCACCCTG AGGCCTGCCA GCGCATTAAC TCCATCCTGA AGTTTTACAC TCTGTGGCGT 
TTTCGCTACC AAGTGTGGCC TCGCATGGAG GAAGGAGCCC AGCAGATCTT TAAGATCCCT 
CCACCCAGCA TTAACTTCAC TCTCCCTTCT CCAATACTGG GCATGCCCTG TGTCCCCATA 
TTTGACCCTC CCTGGGTGCC CCAGAACACT GGCAGCGTCC AGGATCCAAT CAACGAAGAC 
CAGTCTTACC CCACATTAAT TCAGTCAGTC CATGCCATAA AATCCTTCTC TGCGCGGGCG 
GTGTCGCGCT CCCACCAGCG AGCCGAGCAC ATCCTCAAGA ACCTGCAGCA GGAGGAGGAG 
AAGCGGCGTC TGGGCCGCGA GGCCAGCATC ATCACAGCCA TCCCTGTGGC TCAGGAGGCC 
TGCTACGAAC CCACATGCAG CCCTCCACCC GAGCAAGAGG AGGAAGCAGA AGAAGTGGTG 
AACCTGGCAT CACGACGTCT GTCTGTCAGC CCATCCTGCG CCTCCAGCAA CTCCCACAGG 
AACTACTCTT TCCGCCGGGG CTCTGTATGG TCTGTCCGCT CGCTGGCCAG TGCTGAAGAT 
GAAGAAAACA CAACAGAACA CACACCCACT CACCACATGC TACAGCCACC TCAAGCTGTC 
TTCCCAGCAT GCATCTGTGC TGCCGTCCTA CCAATAGTGC ACCTCATGGA AGACGGTGAA 
GTTAGAGAGG ATGGCGTGGC TGTGAGTGCT GTTGCCCAGC AAATCCTCTG GAACTGCCTC 
ATTGAAGATC CAGCTCTCGT TCTCCGCCAC TTCCTGGAAA AGCTGACAGT TAGCAACAGA 
CAGGACGAGC TGATGTACAT GCTTAGGAAG CTCCTGCTCA ACATTGGAGA TCTGCCAGCC 
CAGACCTCTC ACATCCTTTT TAACTACCTG GTGGGCCTGA TCATGTATTT TGTGCGGACT 
CCCTGTGAAT GGGGCATGGA CGCCATCTCG GCCACGCTGA CCTTCCTCTG GGAGGTGGTC 
GGCTACGTGG AGGGGCTCTT CTTTAAAGAC CTGAAGCAGA CCATGAAGAA AGAGCAGTGT 
GAGGTCAAAC TGCTGGTCAC AGCATCGATG CCAGGAACCA AAACGCTGGT GGTGCACGGG 
CAGAATGAAT GTGACATCCC AACCCAGCTA CCAGTCCACG AAGACACTCA GTTTGAAGCC 
CTGCTAAAGG AGTGTCTGGA ATTTTTCAAC ATTCCCGAGG CCAGATCGGC ACACTACTTC 
CTCATGGATA AACGATGGAA CCTCATCCAT TATTCAAAGA CATATGTTAG AGACATCTAC 
CCGTTCCGAA GGTCAGTCTC TCCTCAGCTC AACCTGGTCC ACATGTTGCC TGAGAAAGGA 
CAGGAGCTCA TCCAGAAACA GGTGTTTTCC CGTAAACTAG AAGAAGTCGG TCGAGTCCTC 
TTCCTCATCT CCCTCACACA ACACATGCCA GCTGTGCACA AGCAGTCCCA CGTCTCCCTG 
CTGCAGGAGG ACCTGCTTCG CCTGCCCTCC TTCCCCCGCA CCGCCATCGA TGCAGAGTTC 
TCCCTCTTTA ATGAGCCTCA AGGAAAAGAG CTGTTTGGCT TGGACACTCT CCACAAGGTG 
TTGTGGATCA AACTGTTGGA GGAAATGTTT CTGGGCATGC CCAGTGAGTA CCCGTGGGGC 
GATGAGATGA TGCTGTTCCT TAACGTCTTC AACGGGGCCC TTCTGCTGCA CCCCGAGGAC 
AGCTCCCTGC TGAGGCAGTA CACGGCGACC GCCATCAACA CCGCCGTTCA CTTTAACCAT 
CTGTTCTCCC TGAGCGGGTA CCAGTGGATG CTGCCAACCA TGCTGCAGGT CTATGCAGAC 
TACGAGAGCA ACCCTCTTCT GAGGCAGGGC ATTGAGTTTT GCTGCCGGCA GTTTTACATC 
CTCCATCGAA AACCCTTTAT CCTGCAGCTG TTTGCCAGCG TTGCTCCGCT GCTCGAGTTC 
ACAACCAGCA CCAGTACCGG TCTTTCTAAA GGAGTTTCGG CCCAGTGTCT GTTCGACCTG 
CTGGTCTCTC TAGAAGGTGA AACCCAGGAT GCCCTGGATG CTCTGGAGCT GGTAAAAGCT 
GAGAAGCCTC TACGCTCTCT GGATTTCTGT TATGGTAATG AGGACCTGAC CTTTTCTATC 
AGCGAGGCCA TCAAGCTGTG CGTCACTGTG GTTGCATATG CCCCCGAATC CTTCAGGAGT 
CTCCAGATGC TGATGGTGCT GGAGGCCTTG GTTCCCTGCT ACCTCCAGAA ACTGAAGAGC 
AACACAGTTA CGATGGAGTC TGCTTCGGCT GCCAGAGATG AGATTGCGGC CATCGCTGCC 
CTAGCTACGT CTCTGCAGGC CCTGCTCTAC AGCTCTGAAA CCCTCACAAG GCCCATGACG 
GCTCCCCAGA TATCTCGCTG TGATCAGGGC CACAAGGGCG GCACTACAGC TAACCACGCC 
ATGTCCGGAG GGGTCAACAC CAGGGACAAT CTTCACCTGC TGGAGGAGGG CCAGGGCATG 
CCAAGGGAAG AGCTGGACGA GCGCATTGCC AGGGAGGAGT TCCGCCGTCC ACGGGAGTCC 
CTGTTGAACA TCTGCACCGA GTTTTACAAA CACTGCGGGC CACGGCTCAA AATCCTGCAG 
AACGTTGCAG GCGAGCCGCG GGTCACAGCT CTGGAGCTAC TGGACATCAA GTCCCACATG 
AGGCTGGCAG AGATCGCCCA CGCCCTGCTG AAGCTGGCGC CCTACGACAC CCTGACCATG 
GAGAGCCGAG GGCTGCGGCG TTACATCATG GAGATGCTGC CCATCACCGA CTGGTCGTCC 
GAGGCCGTCC GGCCTGCCCT CATCCTCATC CTCAAGAGGC TGGACCGCAT GTTCAACAAG 
ATCCACAAGA TGCCCACGCT CAGGTGCGCT CGCCCTCAGG CTTTATGCAG CAGGAGACAG 
GTGGAGTGGG AGGCGGCCAG TAGCCTGATC GAGGGGATCT GCCTGACCTT GCAGCGACAG 
CCGATCATCT CGTTCCTGCC TCACCTGCGC TCGCTTATCA ACGTCTGCGT CAACCTGGTG 
ATGGGTGTGG TCGGCCCCTC CAGTGTGGCG GACGGGCTGC CACTCCTCCA CCTCAGCCCT 
TACCTGTCTC CGCCTCTGCC CTTTAGCACC GCCGTGGTCC GATTGGTTGC TCTGCAGATT 
CAGGCCTTGA AGGAAGACTT TCCCCTCAGT CACGTGATCT CACCTTTCAC CAATCAGGAA 
AGGCGGGAGG GGATGCTGCT CAACCTGCTC ATTCCCTTCG TGTTAACTGT AGGCTCCGGG 
AGCAAAGACA GCCCCCACCT GGAGCAGCCG GAGGTCTTCC TGCTCCTACA GACAGTCATC 
AACATCCTGC TTCCCCCTCG TATCATCTCC ACATCCCGCA CCAAGAACTT TATGCTTGAC 
GCCTCTCCGG CTCACTGCTC CACGCCGGGT GACCCTGGGA AAGATCTGCG TAGGGAGGGA 
TTAGCAGAGT CCACGAGCCA GGCTGCTTAT TTAGCTCTGA AGGTGGTGCT GGTTTGCTTC 
GAGCGCTCGC TGGGGAACCA GTGGTATCGG CTGAGCCTGC AGGTGAAGGA GATGGCTCTG 
AGGAAGGTGG GCGGCTTGGC CTTCTGGGAC TTCATCGACT TTATCGTCCG AACCCGGATC 
CCTATTTTTG TCCTGCTCAG ACCCTTTATA CAGTGCAAGT TGCTGACCCA GCCGGCCGAT 
TCCCAGGAAG AGATCACAGC GCGCCACCAC ATCGCTGATC AGCTGGAGCG GCGCTTCATC 
CCACGACCTC TCTGCAAGAG CTCACTGTTT GCAGAGTTCA ACAATGAGCT CAAGATCCTC 
AAAGAGGCTG TGCACAGCGG CTCAGCATAT CAGGGCAAGA CATCCATCAG CACGGTGGGC 
ACCTCCACGT CAGCCTACCG CCTCAGTTTG GCCACAATGT CGCGATCCAA CACAGGCACT 
GGAACAGTTT GGGAGCAGGA CAGCCAACCG TCACGGCAGC CCTCACAGGA CACGCTCAGC 
CGTACCGACG AGGACGATGA AGAGATTGAC TCCATGAGCA TTCCCAGCGT GGTGAGCGAG 
CACGAAGCCT TCATGCCCAG GGTGATGTCA CAGCGCCGTT TCTCCAGCCA CGCCACTGGC 
TCGGCGGCCT CTCAGCCCGA GGCCCCCCGC ACCACCATGT TGCCCAGCCA CAGTGAACCC 
AATGTGCTAG ATGAGTCCCA GGGTCTTCTG CAGGAGGGTA ACCTCTCTAG GGTGGCCAGT 
GTGCAGAGCG AACCAGGTCA GAACCTCCTG ATCCAGCCCC CGTTAGGAAG AAAGCGAGGA 
CTCAGACAGA TGCGACGCCC CCTGCTGTCC ATTCCAAAAA CCGAACCACG CGGTCGATCT 
GGAGCGAGGC TTTCTACAAC GCGCAGGAGC ATCCAGCCCA AGAACAAACC ACTGGAAACT 
TTATTGGACT GTGAAGCGCA TGGAGACCAA AAGAGGTCAG TCACCTTTAC AGAGAATCAA 
GGCCAGCAGG CATCCACGGG GGCAAGCAAG CCTCCAACTC CCAGCACTGT GGACCCACCG 
GGCGAAGGCA GAGGGACGAG TCCTGCCCCA TCAAAGTCCC CAACCCTGGA AGTACCCCCC 
ACCTCTTCAG CCATGATCTT GGCTGACCTG CACGGACCTA CAAATGCACA GGTCCCTCAA 
GCATTAAGCA GCAAGGAGAA GAGAGAGCAG TGGGGGCTAC GCAGCAGCCT CTCCCCTCCC 
CCATCCATCT CCCGGCCTTC CACTCCAACA CCCCCATCCC GAACCTGCTC CCCTCTCCCC 
CTCTCCAGAA CCTGCTCCCC TCTCCCTTTG TCCCGCACCT CCTCTCCTCT TCCACCGCCA 
CTCCCAGTGC TGAGCACAGC ACCCGCTCCG GGTCCACCGC CGCCACCTCC ATTACCACCA 
ACTCATCTTG CTGCTGCTCC TCCTCCTCCA CCACCCCCGG GGCCTTCTAG GATCAGAGAG 
AGCCCCGGGG ACGAGGACAC AACCGCCCTG CTGCCGCGCA GCGACACCCT GCTGCACCTC 
AGCGAGGACG ATGGCACCGA GAACCCGCTT CTCACCCCGC TGCTGTCCCC TCCTCCCCCC 
CGCCGGTCGC CCCGCCGCTC TCCCCTCCCC CTCTCACCCG TCGACCTGGA CCTGGATGAG 
TCCCACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922261.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777715.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.

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CloneID	OMa370059
Clone ID Related Accession (Same CDS sequence)	XM_012922261.4
Accession Version	XM_012922261.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922261.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GATGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777715.2
CDS	397..10779
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDKYGSAGERSRRAKKRNIVLSAMTKPPAVSSMVSPASLLLPDKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922261.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922261.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAAGTA CGGCAGTGCA 
GGTGAGAGGT CCAGGAGGGC GAAAAAAAGA AACATCGTGC TGTCAGCAAT GACTAAACCA 
CCTGCTGTGT CATCCATGGT CTCTCCTGCC TCCCTGCTGC TTCCAGACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGGGTGGCCA GTGTGCAGAG CGAACCAGGT CAGAACCTCC TGATCCAGCC CCCGTTAGGA 
AGAAAGCGAG GACTCAGACA GATGCGACGC CCCCTGCTGT CCATTCCAAA AACCGAACCA 
CGCGGTCGAT CTGGAGCGAG GCTTTCTACA ACGCGCAGGA GCATCCAGCC CAAGAACAAA 
CCACTGGCGC ATGGAGACCA AAAGAGGTCA GTCACCTTTA CAGAGAATCA AGGCCAGCAG 
GCATCCACGG GGGCAAGCAA GCCTCCAACT CCCAGCACTG TGGACCCACC GGGCGAAGGC 
AGAGGGACGA GTCCTGCCCC ATCAAAGTCC CCAACCCTGG AAGTACCCCC CACCTCTTCA 
GCCATGATCT TGGCTGACCT GCACGGACCT ACAAATGCAC AGGTCCCTCA AGCATTAAGC 
AGCAAGGAGA AGAGAGAGCA GTGGGGGCTA CGCAGCAGCC TCTCCCCTCC CCCATCCATC 
TCCCGGCCTT CCACTCCAAC ACCCCCATCC CGAACCTGCT CCCCTCTCCC CCTCTCCAGA 
ACCTGCTCCC CTCTCCCTTT GTCCCGCACC TCCTCTCCTC TTCCACCGCC ACTCCCAGTG 
CTGAGCACAG CACCCGCTCC GGGTCCACCG CCGCCACCTC CATTACCACC AACTCATCTT 
GCTGCTGCTC CTCCTCCTCC ACCACCCCCG GGGCCTTCTA GGATCAGAGA GAGCCCCGGG 
GACGAGGACA CAACCGCCCT GCTGCCGCGC AGCGACACCC TGCTGCACCT CAGCGAGGAC 
GATGGCACCG AGAACCCGCT TCTCACCCCG CTGCTGTCCC CTCCTCCCCC CCGCCGGTCG 
CCCCGCCGCT CTCCCCTCCC CCTCTCACCC GTCGACCTGG ACCTGGATGA GTCCCACGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_004562584.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004562641.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.

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CloneID	OMa370060
Clone ID Related Accession (Same CDS sequence)	XM_004562584.4
Accession Version	XM_004562584.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004562584.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004562641.2
CDS	397..10767
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGKWRSGRRNPSKLHHTEEKDGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_004562584.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004562584.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGT AAATGGAGGT CTGGCAGGAG GAATCCATCC 
AAGCTTCACC ACACAGAAGA GAAAGATGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTTACCC CACATTAATT 
CAGTCAGTCC ATGCCATAAA ATCCTTCTCT GCGCGGGCGG TGTCGCGCTC CCACCAGCGA 
GCCGAGCACA TCCTCAAGAA CCTGCAGCAG GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG 
GCCAGCATCA TCACAGCCAT CCCTGTGGCT CAGGAGGCCT GCTACGAACC CACATGCAGC 
CCTCCACCCG AGCAAGAGGA GGAAGCAGAA GAAGTGGTGA ACCTGGCATC ACGACGTCTG 
TCTGTCAGCC CATCCTGCGC CTCCAGCAAC TCCCACAGGA ACTACTCTTT CCGCCGGGGC 
TCTGTATGGT CTGTCCGCTC GCTGGCCAGT GCTGAAGATG AAGAAAACAC AACAGAACAC 
ACACCCACTC ACCACATGCT ACAGCCACCT CAAGCTGTCT TCCCAGCATG CATCTGTGCT 
GCCGTCCTAC CAATAGTGCA CCTCATGGAA GACGGTGAAG TTAGAGAGGA TGGCGTGGCT 
GTGAGTGCTG TTGCCCAGCA AATCCTCTGG AACTGCCTCA TTGAAGATCC AGCTCTCGTT 
CTCCGCCACT TCCTGGAAAA GCTGACAGTT AGCAACAGAC AGGACGAGCT GATGTACATG 
CTTAGGAAGC TCCTGCTCAA CATTGGAGAT CTGCCAGCCC AGACCTCTCA CATCCTTTTT 
AACTACCTGG TGGGCCTGAT CATGTATTTT GTGCGGACTC CCTGTGAATG GGGCATGGAC 
GCCATCTCGG CCACGCTGAC CTTCCTCTGG GAGGTGGTCG GCTACGTGGA GGGGCTCTTC 
TTTAAAGACC TGAAGCAGAC CATGAAGAAA GAGCAGTGTG AGGTCAAACT GCTGGTCACA 
GCATCGATGC CAGGAACCAA AACGCTGGTG GTGCACGGGC AGAATGAATG TGACATCCCA 
ACCCAGCTAC CAGTCCACGA AGACACTCAG TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA 
TTTTTCAACA TTCCCGAGGC CAGATCGGCA CACTACTTCC TCATGGATAA ACGATGGAAC 
CTCATCCATT ATTCAAAGAC ATATGTTAGA GACATCTACC CGTTCCGAAG GTCAGTCTCT 
CCTCAGCTCA ACCTGGTCCA CATGTTGCCT GAGAAAGGAC AGGAGCTCAT CCAGAAACAG 
GTGTTTTCCC GTAAACTAGA AGAAGTCGGT CGAGTCCTCT TCCTCATCTC CCTCACACAA 
CACATGCCAG CTGTGCACAA GCAGTCCCAC GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC 
CTGCCCTCCT TCCCCCGCAC CGCCATCGAT GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA 
GGAAAAGAGC TGTTTGGCTT GGACACTCTC CACAAGGTGT TGTGGATCAA ACTGTTGGAG 
GAAATGTTTC TGGGCATGCC CAGTGAGTAC CCGTGGGGCG ATGAGATGAT GCTGTTCCTT 
AACGTCTTCA ACGGGGCCCT TCTGCTGCAC CCCGAGGACA GCTCCCTGCT GAGGCAGTAC 
ACGGCGACCG CCATCAACAC CGCCGTTCAC TTTAACCATC TGTTCTCCCT GAGCGGGTAC 
CAGTGGATGC TGCCAACCAT GCTGCAGGTC TATGCAGACT ACGAGAGCAA CCCTCTTCTG 
AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG TTTTACATCC TCCATCGAAA ACCCTTTATC 
CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG CTCGAGTTCA CAACCAGCAC CAGTACCGGT 
CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG TTCGACCTGC TGGTCTCTCT AGAAGGTGAA 
ACCCAGGATG CCCTGGATGC TCTGGAGCTG GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG 
GATTTCTGTT ATGGTAATGA GGACCTGACC TTTTCTATCA GCGAGGCCAT CAAGCTGTGC 
GTCACTGTGG TTGCATATGC CCCCGAATCC TTCAGGAGTC TCCAGATGCT GATGGTGCTG 
GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA CTGAAGAGCA ACACAGTTAC GATGGAGTCT 
GCTTCGGCTG CCAGAGATGA GATTGCGGCC ATCGCTGCCC TAGCTACGTC TCTGCAGGCC 
CTGCTCTACA GCTCTGAAAC CCTCACAAGG CCCATGACGG CTCCCCAGAT ATCTCGCTGT 
GATCAGGGCC ACAAGGGCGG CACTACAGCT AACCACGCCA TGTCCGGAGG GGTCAACACC 
AGGGACAATC TTCACCTGCT GGAGGAGGGC CAGGGCATGC CAAGGGAAGA GCTGGACGAG 
CGCATTGCCA GGGAGGAGTT CCGCCGTCCA CGGGAGTCCC TGTTGAACAT CTGCACCGAG 
TTTTACAAAC ACTGCGGGCC ACGGCTCAAA ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG 
GTCACAGCTC TGGAGCTACT GGACATCAAG TCCCACATGA GGCTGGCAGA GATCGCCCAC 
GCCCTGCTGA AGCTGGCGCC CTACGACACC CTGACCATGG AGAGCCGAGG GCTGCGGCGT 
TACATCATGG AGATGCTGCC CATCACCGAC TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC 
ATCCTCATCC TCAAGAGGCT GGACCGCATG TTCAACAAGA TCCACAAGAT GCCCACGCTC 
AGGTGCGCTC GCCCTCAGGC TTTATGCAGC AGGAGACAGG TGGAGTGGGA GGCGGCCAGT 
AGCCTGATCG AGGGGATCTG CCTGACCTTG CAGCGACAGC CGATCATCTC GTTCCTGCCT 
CACCTGCGCT CGCTTATCAA CGTCTGCGTC AACCTGGTGA TGGGTGTGGT CGGCCCCTCC 
AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC 
TTTAGCACCG CCGTGGTCCG ATTGGTTGCT CTGCAGATTC AGGCCTTGAA GGAAGACTTT 
CCCCTCAGTC ACGTGATCTC ACCTTTCACC AATCAGGAAA GGCGGGAGGG GATGCTGCTC 
AACCTGCTCA TTCCCTTCGT GTTAACTGTA GGCTCCGGGA GCAAAGACAG CCCCCACCTG 
GAGCAGCCGG AGGTCTTCCT GCTCCTACAG ACAGTCATCA ACATCCTGCT TCCCCCTCGT 
ATCATCTCCA CATCCCGCAC CAAGAACTTT ATGCTTGACG CCTCTCCGGC TCACTGCTCC 
ACGCCGGGTG ACCCTGGGAA AGATCTGCGT AGGGAGGGAT TAGCAGAGTC CACGAGCCAG 
GCTGCTTATT TAGCTCTGAA GGTGGTGCTG GTTTGCTTCG AGCGCTCGCT GGGGAACCAG 
TGGTATCGGC TGAGCCTGCA GGTGAAGGAG ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC 
TTCTGGGACT TCATCGACTT TATCGTCCGA ACCCGGATCC CTATTTTTGT CCTGCTCAGA 
CCCTTTATAC AGTGCAAGTT GCTGACCCAG CCGGCCGATT CCCAGGAAGA GATCACAGCG 
CGCCACCACA TCGCTGATCA GCTGGAGCGG CGCTTCATCC CACGACCTCT CTGCAAGAGC 
TCACTGTTTG CAGAGTTCAA CAATGAGCTC AAGATCCTCA AAGAGGCTGT GCACAGCGGC 
TCAGCATATC AGGGCAAGAC ATCCATCAGC ACGGTGGGCA CCTCCACGTC AGCCTACCGC 
CTCAGTTTGG CCACAATGTC GCGATCCAAC ACAGGCACTG GAACAGTTTG GGAGCAGGAC 
AGCCAACCGT CACGGCAGCC CTCACAGGAC ACGCTCAGCC GTACCGACGA GGACGATGAA 
GAGATTGACT CCATGAGCAT TCCCAGCGTG GTGAGCGAGC ACGAAGCCTT CATGCCCAGG 
GTGATGTCAC AGCGCCGTTT CTCCAGCCAC GCCACTGGCT CGGCGGCCTC TCAGCCCGAG 
GCCCCCCGCA CCACCATGTT GCCCAGCCAC AGTGAACCCA ATGTGCTAGA TGAGTCCCAG 
GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGAAACTT TATTGGACTG TGAAGCGCAT 
GGAGACCAAA AGAGGTCAGT CACCTTTACA GAGAATCAAG GCCAGCAGGC ATCCACGGGG 
GCAAGCAAGC CTCCAACTCC CAGCACTGTG GACCCACCGG GCGAAGGCAG AGGGACGAGT 
CCTGCCCCAT CAAAGTCCCC AACCCTGGAA GTACCCCCCA CCTCTTCAGC CATGATCTTG 
GCTGACCTGC ACGGACCTAC AAATGCACAG GTCCCTCAAG CATTAAGCAG CAAGGAGAAG 
AGAGAGCAGT GGGGGCTACG CAGCAGCCTC TCCCCTCCCC CATCCATCTC CCGGCCTTCC 
ACTCCAACAC CCCCATCCCG AACCTGCTCC CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT 
CTCCCTTTGT CCCGCACCTC CTCTCCTCTT CCACCGCCAC TCCCAGTGCT GAGCACAGCA 
CCCGCTCCGG GTCCACCGCC GCCACCTCCA TTACCACCAA CTCATCTTGC TGCTGCTCCT 
CCTCCTCCAC CACCCCCGGG GCCTTCTAGG ATCAGAGAGA GCCCCGGGGA CGAGGACACA 
ACCGCCCTGC TGCCGCGCAG CGACACCCTG CTGCACCTCA GCGAGGACGA TGGCACCGAG 
AACCCGCTTC TCACCCCGCT GCTGTCCCCT CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT 
CCCCTCCCCC TCTCACCCGT CGACCTGGAC CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922262.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777716.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.

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CloneID	OMa370062
Clone ID Related Accession (Same CDS sequence)	XM_012922262.4
Accession Version	XM_012922262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGTGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777716.2
CDS	397..10677
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSYPTLIQSVHAIKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRCARPQALCSRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922262.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922262.4

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTTACCC CACATTAATT CAGTCAGTCC ATGCCATAAA ATCCTTCTCT 
GCGCGGGCGG TGTCGCGCTC CCACCAGCGA GCCGAGCACA TCCTCAAGAA CCTGCAGCAG 
GAGGAGGAGA AGCGGCGTCT GGGCCGCGAG GCCAGCATCA TCACAGCCAT CCCTGTGGCT 
CAGGAGGCCT GCTACGAACC CACATGCAGC CCTCCACCCG AGCAAGAGGA GGAAGCAGAA 
GAAGTGGTGA ACCTGGCATC ACGACGTCTG TCTGTCAGCC CATCCTGCGC CTCCAGCAAC 
TCCCACAGGA ACTACTCTTT CCGCCGGGGC TCTGTATGGT CTGTCCGCTC GCTGGCCAGT 
GCTGAAGATG AAGAAAACAC AACAGAACAC ACACCCACTC ACCACATGCT ACAGCCACCT 
CAAGCTGTCT TCCCAGCATG CATCTGTGCT GCCGTCCTAC CAATAGTGCA CCTCATGGAA 
GACGGTGAAG TTAGAGAGGA TGGCGTGGCT GTGAGTGCTG TTGCCCAGCA AATCCTCTGG 
AACTGCCTCA TTGAAGATCC AGCTCTCGTT CTCCGCCACT TCCTGGAAAA GCTGACAGTT 
AGCAACAGAC AGGACGAGCT GATGTACATG CTTAGGAAGC TCCTGCTCAA CATTGGAGAT 
CTGCCAGCCC AGACCTCTCA CATCCTTTTT AACTACCTGG TGGGCCTGAT CATGTATTTT 
GTGCGGACTC CCTGTGAATG GGGCATGGAC GCCATCTCGG CCACGCTGAC CTTCCTCTGG 
GAGGTGGTCG GCTACGTGGA GGGGCTCTTC TTTAAAGACC TGAAGCAGAC CATGAAGAAA 
GAGCAGTGTG AGGTCAAACT GCTGGTCACA GCATCGATGC CAGGAACCAA AACGCTGGTG 
GTGCACGGGC AGAATGAATG TGACATCCCA ACCCAGCTAC CAGTCCACGA AGACACTCAG 
TTTGAAGCCC TGCTAAAGGA GTGTCTGGAA TTTTTCAACA TTCCCGAGGC CAGATCGGCA 
CACTACTTCC TCATGGATAA ACGATGGAAC CTCATCCATT ATTCAAAGAC ATATGTTAGA 
GACATCTACC CGTTCCGAAG GTCAGTCTCT CCTCAGCTCA ACCTGGTCCA CATGTTGCCT 
GAGAAAGGAC AGGAGCTCAT CCAGAAACAG GTGTTTTCCC GTAAACTAGA AGAAGTCGGT 
CGAGTCCTCT TCCTCATCTC CCTCACACAA CACATGCCAG CTGTGCACAA GCAGTCCCAC 
GTCTCCCTGC TGCAGGAGGA CCTGCTTCGC CTGCCCTCCT TCCCCCGCAC CGCCATCGAT 
GCAGAGTTCT CCCTCTTTAA TGAGCCTCAA GGAAAAGAGC TGTTTGGCTT GGACACTCTC 
CACAAGGTGT TGTGGATCAA ACTGTTGGAG GAAATGTTTC TGGGCATGCC CAGTGAGTAC 
CCGTGGGGCG ATGAGATGAT GCTGTTCCTT AACGTCTTCA ACGGGGCCCT TCTGCTGCAC 
CCCGAGGACA GCTCCCTGCT GAGGCAGTAC ACGGCGACCG CCATCAACAC CGCCGTTCAC 
TTTAACCATC TGTTCTCCCT GAGCGGGTAC CAGTGGATGC TGCCAACCAT GCTGCAGGTC 
TATGCAGACT ACGAGAGCAA CCCTCTTCTG AGGCAGGGCA TTGAGTTTTG CTGCCGGCAG 
TTTTACATCC TCCATCGAAA ACCCTTTATC CTGCAGCTGT TTGCCAGCGT TGCTCCGCTG 
CTCGAGTTCA CAACCAGCAC CAGTACCGGT CTTTCTAAAG GAGTTTCGGC CCAGTGTCTG 
TTCGACCTGC TGGTCTCTCT AGAAGGTGAA ACCCAGGATG CCCTGGATGC TCTGGAGCTG 
GTAAAAGCTG AGAAGCCTCT ACGCTCTCTG GATTTCTGTT ATGGTAATGA GGACCTGACC 
TTTTCTATCA GCGAGGCCAT CAAGCTGTGC GTCACTGTGG TTGCATATGC CCCCGAATCC 
TTCAGGAGTC TCCAGATGCT GATGGTGCTG GAGGCCTTGG TTCCCTGCTA CCTCCAGAAA 
CTGAAGAGCA ACACAGTTAC GATGGAGTCT GCTTCGGCTG CCAGAGATGA GATTGCGGCC 
ATCGCTGCCC TAGCTACGTC TCTGCAGGCC CTGCTCTACA GCTCTGAAAC CCTCACAAGG 
CCCATGACGG CTCCCCAGAT ATCTCGCTGT GATCAGGGCC ACAAGGGCGG CACTACAGCT 
AACCACGCCA TGTCCGGAGG GGTCAACACC AGGGACAATC TTCACCTGCT GGAGGAGGGC 
CAGGGCATGC CAAGGGAAGA GCTGGACGAG CGCATTGCCA GGGAGGAGTT CCGCCGTCCA 
CGGGAGTCCC TGTTGAACAT CTGCACCGAG TTTTACAAAC ACTGCGGGCC ACGGCTCAAA 
ATCCTGCAGA ACGTTGCAGG CGAGCCGCGG GTCACAGCTC TGGAGCTACT GGACATCAAG 
TCCCACATGA GGCTGGCAGA GATCGCCCAC GCCCTGCTGA AGCTGGCGCC CTACGACACC 
CTGACCATGG AGAGCCGAGG GCTGCGGCGT TACATCATGG AGATGCTGCC CATCACCGAC 
TGGTCGTCCG AGGCCGTCCG GCCTGCCCTC ATCCTCATCC TCAAGAGGCT GGACCGCATG 
TTCAACAAGA TCCACAAGAT GCCCACGCTC AGGTGCGCTC GCCCTCAGGC TTTATGCAGC 
AGGAGACAGG TGGAGTGGGA GGCGGCCAGT AGCCTGATCG AGGGGATCTG CCTGACCTTG 
CAGCGACAGC CGATCATCTC GTTCCTGCCT CACCTGCGCT CGCTTATCAA CGTCTGCGTC 
AACCTGGTGA TGGGTGTGGT CGGCCCCTCC AGTGTGGCGG ACGGGCTGCC ACTCCTCCAC 
CTCAGCCCTT ACCTGTCTCC GCCTCTGCCC TTTAGCACCG CCGTGGTCCG ATTGGTTGCT 
CTGCAGATTC AGGCCTTGAA GGAAGACTTT CCCCTCAGTC ACGTGATCTC ACCTTTCACC 
AATCAGGAAA GGCGGGAGGG GATGCTGCTC AACCTGCTCA TTCCCTTCGT GTTAACTGTA 
GGCTCCGGGA GCAAAGACAG CCCCCACCTG GAGCAGCCGG AGGTCTTCCT GCTCCTACAG 
ACAGTCATCA ACATCCTGCT TCCCCCTCGT ATCATCTCCA CATCCCGCAC CAAGAACTTT 
ATGCTTGACG CCTCTCCGGC TCACTGCTCC ACGCCGGGTG ACCCTGGGAA AGATCTGCGT 
AGGGAGGGAT TAGCAGAGTC CACGAGCCAG GCTGCTTATT TAGCTCTGAA GGTGGTGCTG 
GTTTGCTTCG AGCGCTCGCT GGGGAACCAG TGGTATCGGC TGAGCCTGCA GGTGAAGGAG 
ATGGCTCTGA GGAAGGTGGG CGGCTTGGCC TTCTGGGACT TCATCGACTT TATCGTCCGA 
ACCCGGATCC CTATTTTTGT CCTGCTCAGA CCCTTTATAC AGTGCAAGTT GCTGACCCAG 
CCGGCCGATT CCCAGGAAGA GATCACAGCG CGCCACCACA TCGCTGATCA GCTGGAGCGG 
CGCTTCATCC CACGACCTCT CTGCAAGAGC TCACTGTTTG CAGAGTTCAA CAATGAGCTC 
AAGATCCTCA AAGAGGCTGT GCACAGCGGC TCAGCATATC AGGGCAAGAC ATCCATCAGC 
ACGGTGGGCA CCTCCACGTC AGCCTACCGC CTCAGTTTGG CCACAATGTC GCGATCCAAC 
ACAGGCACTG GAACAGTTTG GGAGCAGGAC AGCCAACCGT CACGGCAGCC CTCACAGGAC 
ACGCTCAGCC GTACCGACGA GGACGATGAA GAGATTGACT CCATGAGCAT TCCCAGCGTG 
GTGAGCGAGC ACGAAGCCTT CATGCCCAGG GTGATGTCAC AGCGCCGTTT CTCCAGCCAC 
GCCACTGGCT CGGCGGCCTC TCAGCCCGAG GCCCCCCGCA CCACCATGTT GCCCAGCCAC 
AGTGAACCCA ATGTGCTAGA TGAGTCCCAG GGTCTTCTGC AGGAGGGTAA CCTCTCTAGG 
GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG AACCTCCTGA TCCAGCCCCC GTTAGGAAGA 
AAGCGAGGAC TCAGACAGAT GCGACGCCCC CTGCTGTCCA TTCCAAAAAC CGAACCACGC 
GGTCGATCTG GAGCGAGGCT TTCTACAACG CGCAGGAGCA TCCAGCCCAA GAACAAACCA 
CTGGAAACTT TATTGGACTG TGAAGCGCAT GGAGACCAAA AGAGGTCAGT CACCTTTACA 
GAGAATCAAG GCCAGCAGGC ATCCACGGGG GCAAGCAAGC CTCCAACTCC CAGCACTGTG 
GACCCACCGG GCGAAGGCAG AGGGACGAGT CCTGCCCCAT CAAAGTCCCC AACCCTGGAA 
GTACCCCCCA CCTCTTCAGC CATGATCTTG GCTGACCTGC ACGGACCTAC AAATGCACAG 
GTCCCTCAAG CATTAAGCAG CAAGGAGAAG AGAGAGCAGT GGGGGCTACG CAGCAGCCTC 
TCCCCTCCCC CATCCATCTC CCGGCCTTCC ACTCCAACAC CCCCATCCCG AACCTGCTCC 
CCTCTCCCCC TCTCCAGAAC CTGCTCCCCT CTCCCTTTGT CCCGCACCTC CTCTCCTCTT 
CCACCGCCAC TCCCAGTGCT GAGCACAGCA CCCGCTCCGG GTCCACCGCC GCCACCTCCA 
TTACCACCAA CTCATCTTGC TGCTGCTCCT CCTCCTCCAC CACCCCCGGG GCCTTCTAGG 
ATCAGAGAGA GCCCCGGGGA CGAGGACACA ACCGCCCTGC TGCCGCGCAG CGACACCCTG 
CTGCACCTCA GCGAGGACGA TGGCACCGAG AACCCGCTTC TCACCCCGCT GCTGTCCCCT 
CCTCCCCCCC GCCGGTCGCC CCGCCGCTCT CCCCTCCCCC TCTCACCCGT CGACCTGGAC 
CTGGATGAGT CCCACGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922263.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777717.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.

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CloneID	OMa370064
Clone ID Related Accession (Same CDS sequence)	XM_012922263.3
Accession Version	XM_012922263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTAAATC CTTCTCTGCG CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC 
GAGCACATCC TCAAGAACCT GCAGCAGGAG GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC 
AGCATCATCA CAGCCATCCC TGTGGCTCAG GAGGCCTGCT ACGAACCCAC ATGCAGCCCT 
CCACCCGAGC AAGAGGAGGA AGAAGAAGTG GTGAACCTGG CATCACGACG TCTGTCTGTC 
AGCCCATCCT GCGCCTCCAG CAACTCCCAC AGGAACTACT CTTTCCGCCG GGGCTCTGTA 
TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA GATGAAGAAA ACACAACAGA ACACACACCC 
ACTCACCACA TGCTACAGCC ACCTCAAGCT GTCTTCCCAG CATGCATCTG TGCTGCCGTC 
CTACCAATAG TGCACCTCAT GGAAGACGGT GAAGTTAGAG AGGATGGCGT GGCTGTGAGT 
GCTGTTGCCC AGCAAATCCT CTGGAACTGC CTCATTGAAG ATCCAGCTCT CGTTCTCCGC 
CACTTCCTGG AAAAGCTGAC AGTTAGCAAC AGACAGGACG AGCTGATGTA CATGCTTAGG 
AAGCTCCTGC TCAACATTGG AGATCTGCCA GCCCAGACCT CTCACATCCT TTTTAACTAC 
CTGGTGGGCC TGATCATGTA TTTTGTGCGG ACTCCCTGTG AATGGGGCAT GGACGCCATC 
TCGGCCACGC TGACCTTCCT CTGGGAGGTG GTCGGCTACG TGGAGGGGCT CTTCTTTAAA 
GACCTGAAGC AGACCATGAA GAAAGAGCAG TGTGAGGTCA AACTGCTGGT CACAGCATCG 
ATGCCAGGAA CCAAAACGCT GGTGGTGCAC GGGCAGAATG AATGTGACAT CCCAACCCAG 
CTACCAGTCC ACGAAGACAC TCAGTTTGAA GCCCTGCTAA AGGAGTGTCT GGAATTTTTC 
AACATTCCCG AGGCCAGATC GGCACACTAC TTCCTCATGG ATAAACGATG GAACCTCATC 
CATTATTCAA AGACATATGT TAGAGACATC TACCCGTTCC GAAGGTCAGT CTCTCCTCAG 
CTCAACCTGG TCCACATGTT GCCTGAGAAA GGACAGGAGC TCATCCAGAA ACAGGTGTTT 
TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC CTCTTCCTCA TCTCCCTCAC ACAACACATG 
CCAGCTGTGC ACAAGCAGTC CCACGTCTCC CTGCTGCAGG AGGACCTGCT TCGCCTGCCC 
TCCTTCCCCC GCACCGCCAT CGATGCAGAG TTCTCCCTCT TTAATGAGCC TCAAGGAAAA 
GAGCTGTTTG GCTTGGACAC TCTCCACAAG GTGTTGTGGA TCAAACTGTT GGAGGAAATG 
TTTCTGGGCA TGCCCAGTGA GTACCCGTGG GGCGATGAGA TGATGCTGTT CCTTAACGTC 
TTCAACGGGG CCCTTCTGCT GCACCCCGAG GACAGCTCCC TGCTGAGGCA GTACACGGCG 
ACCGCCATCA ACACCGCCGT TCACTTTAAC CATCTGTTCT CCCTGAGCGG GTACCAGTGG 
ATGCTGCCAA CCATGCTGCA GGTCTATGCA GACTACGAGA GCAACCCTCT TCTGAGGCAG 
GGCATTGAGT TTTGCTGCCG GCAGTTTTAC ATCCTCCATC GAAAACCCTT TATCCTGCAG 
CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG TTCACAACCA GCACCAGTAC CGGTCTTTCT 
AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC CTGCTGGTCT CTCTAGAAGG TGAAACCCAG 
GATGCCCTGG ATGCTCTGGA GCTGGTAAAA GCTGAGAAGC CTCTACGCTC TCTGGATTTC 
TGTTATGGTA ATGAGGACCT GACCTTTTCT ATCAGCGAGG CCATCAAGCT GTGCGTCACT 
GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG AGTCTCCAGA TGCTGATGGT GCTGGAGGCC 
TTGGTTCCCT GCTACCTCCA GAAACTGAAG AGCAACACAG TTACGATGGA GTCTGCTTCG 
GCTGCCAGAG ATGAGATTGC GGCCATCGCT GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC 
TACAGCTCTG AAACCCTCAC AAGGCCCATG ACGGCTCCCC AGATATCTCG CTGTGATCAG 
GGCCACAAGG GCGGCACTAC AGCTAACCAC GCCATGTCCG GAGGGGTCAA CACCAGGGAC 
AATCTTCACC TGCTGGAGGA GGGCCAGGGC ATGCCAAGGG AAGAGCTGGA CGAGCGCATT 
GCCAGGGAGG AGTTCCGCCG TCCACGGGAG TCCCTGTTGA ACATCTGCAC CGAGTTTTAC 
AAACACTGCG GGCCACGGCT CAAAATCCTG CAGAACGTTG CAGGCGAGCC GCGGGTCACA 
GCTCTGGAGC TACTGGACAT CAAGTCCCAC ATGAGGCTGG CAGAGATCGC CCACGCCCTG 
CTGAAGCTGG CGCCCTACGA CACCCTGACC ATGGAGAGCC GAGGGCTGCG GCGTTACATC 
ATGGAGATGC TGCCCATCAC CGACTGGTCG TCCGAGGCCG TCCGGCCTGC CCTCATCCTC 
ATCCTCAAGA GGCTGGACCG CATGTTCAAC AAGATCCACA AGATGCCCAC GCTCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTTCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGATGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777717.2
CDS	397..10611
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLETLLDCEAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922263.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922263.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGCACCAGTG CGGAAGATTC GTATCGGAGG CTCGCGCCTG 
CTTCAGATCA AAGGAGCCCG CAGTTTTCGT GTGAAGAAGG TTGGCTCGCT GTCTTCGATA 
CGCAGAGCGG GGAGTCTGAA GAGCACCAAG CTGTCTCGGC AGGACTCTGA GTCTGAGAAC 
GAAGAAGGCC TGCTGTCACA AACGCAGAGC AGGGACACAG TTACTGACAT CGGCAGTCCC 
TTCAGCACCA GTGAACCCAG CATCGAGCCA GAAGGTCAGA GCTCAGGAGG GACGGAGGAC 
AACTACCACC GCAACATGTC CTGGCTTCAC ATTATGATCC TGCTGTGCAA CCAGCAGAGT 
TTCATCTGTA CCCACATAGA CTTCTGCCAC CCACGCTGCT ACCAACACCA CAGCCGCTCC 
TGCGCCCGTC TGGTCCGTGC CATCAAGCTC GTGTACGGGG AGACGGTGGA CAGCCTGAGG 
GAGGACAGCG CCTCCTCTGG TAACATCGGA GGACGTGTGA AGAAAAACAA AGAGTGTTCA 
GACAAGTCTT GTCTGAGGAC CCCGTCCATG AAGAGAAGAC CCACTGACTC CAACACAGAA 
GGGAAGAAGG ATACAGGCAT GCTCAAGTAC ATCCGCAATC AGGTGATGAG TTTGTCACCA 
GCTCCTCTTT CACTGCTGAT CAAAGCAGCT CCTATCTTGA CTGACGACAT GTACGGAGAC 
ATCCAGCCCG CAGCCTGGGA ACTCCTACTC AGTGTGGATG AACATATGGC AGCCGCTGCT 
GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG GTCCCTGATG CAGTGACCGA AATGATGATG 
GCAGAGTTCC AGCACCCTGA GGCCTGCCAG CGCATTAACT CCATCCTGAA GTTTTACACT 
CTGTGGCGTT TTCGCTACCA AGTGTGGCCT CGCATGGAGG AAGGAGCCCA GCAGATCTTT 
AAGATCCCTC CACCCAGCAT TAACTTCACT CTCCCTTCTC CAATACTGGG CATGCCCTGT 
GTCCCCATAT TTGACCCTCC CTGGGTGCCC CAGAACACTG GCAGCGTCCA GGATCCAATC 
AACGAAGACC AGTCTAAATC CTTCTCTGCG CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC 
GAGCACATCC TCAAGAACCT GCAGCAGGAG GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC 
AGCATCATCA CAGCCATCCC TGTGGCTCAG GAGGCCTGCT ACGAACCCAC ATGCAGCCCT 
CCACCCGAGC AAGAGGAGGA AGAAGAAGTG GTGAACCTGG CATCACGACG TCTGTCTGTC 
AGCCCATCCT GCGCCTCCAG CAACTCCCAC AGGAACTACT CTTTCCGCCG GGGCTCTGTA 
TGGTCTGTCC GCTCGCTGGC CAGTGCTGAA GATGAAGAAA ACACAACAGA ACACACACCC 
ACTCACCACA TGCTACAGCC ACCTCAAGCT GTCTTCCCAG CATGCATCTG TGCTGCCGTC 
CTACCAATAG TGCACCTCAT GGAAGACGGT GAAGTTAGAG AGGATGGCGT GGCTGTGAGT 
GCTGTTGCCC AGCAAATCCT CTGGAACTGC CTCATTGAAG ATCCAGCTCT CGTTCTCCGC 
CACTTCCTGG AAAAGCTGAC AGTTAGCAAC AGACAGGACG AGCTGATGTA CATGCTTAGG 
AAGCTCCTGC TCAACATTGG AGATCTGCCA GCCCAGACCT CTCACATCCT TTTTAACTAC 
CTGGTGGGCC TGATCATGTA TTTTGTGCGG ACTCCCTGTG AATGGGGCAT GGACGCCATC 
TCGGCCACGC TGACCTTCCT CTGGGAGGTG GTCGGCTACG TGGAGGGGCT CTTCTTTAAA 
GACCTGAAGC AGACCATGAA GAAAGAGCAG TGTGAGGTCA AACTGCTGGT CACAGCATCG 
ATGCCAGGAA CCAAAACGCT GGTGGTGCAC GGGCAGAATG AATGTGACAT CCCAACCCAG 
CTACCAGTCC ACGAAGACAC TCAGTTTGAA GCCCTGCTAA AGGAGTGTCT GGAATTTTTC 
AACATTCCCG AGGCCAGATC GGCACACTAC TTCCTCATGG ATAAACGATG GAACCTCATC 
CATTATTCAA AGACATATGT TAGAGACATC TACCCGTTCC GAAGGTCAGT CTCTCCTCAG 
CTCAACCTGG TCCACATGTT GCCTGAGAAA GGACAGGAGC TCATCCAGAA ACAGGTGTTT 
TCCCGTAAAC TAGAAGAAGT CGGTCGAGTC CTCTTCCTCA TCTCCCTCAC ACAACACATG 
CCAGCTGTGC ACAAGCAGTC CCACGTCTCC CTGCTGCAGG AGGACCTGCT TCGCCTGCCC 
TCCTTCCCCC GCACCGCCAT CGATGCAGAG TTCTCCCTCT TTAATGAGCC TCAAGGAAAA 
GAGCTGTTTG GCTTGGACAC TCTCCACAAG GTGTTGTGGA TCAAACTGTT GGAGGAAATG 
TTTCTGGGCA TGCCCAGTGA GTACCCGTGG GGCGATGAGA TGATGCTGTT CCTTAACGTC 
TTCAACGGGG CCCTTCTGCT GCACCCCGAG GACAGCTCCC TGCTGAGGCA GTACACGGCG 
ACCGCCATCA ACACCGCCGT TCACTTTAAC CATCTGTTCT CCCTGAGCGG GTACCAGTGG 
ATGCTGCCAA CCATGCTGCA GGTCTATGCA GACTACGAGA GCAACCCTCT TCTGAGGCAG 
GGCATTGAGT TTTGCTGCCG GCAGTTTTAC ATCCTCCATC GAAAACCCTT TATCCTGCAG 
CTGTTTGCCA GCGTTGCTCC GCTGCTCGAG TTCACAACCA GCACCAGTAC CGGTCTTTCT 
AAAGGAGTTT CGGCCCAGTG TCTGTTCGAC CTGCTGGTCT CTCTAGAAGG TGAAACCCAG 
GATGCCCTGG ATGCTCTGGA GCTGGTAAAA GCTGAGAAGC CTCTACGCTC TCTGGATTTC 
TGTTATGGTA ATGAGGACCT GACCTTTTCT ATCAGCGAGG CCATCAAGCT GTGCGTCACT 
GTGGTTGCAT ATGCCCCCGA ATCCTTCAGG AGTCTCCAGA TGCTGATGGT GCTGGAGGCC 
TTGGTTCCCT GCTACCTCCA GAAACTGAAG AGCAACACAG TTACGATGGA GTCTGCTTCG 
GCTGCCAGAG ATGAGATTGC GGCCATCGCT GCCCTAGCTA CGTCTCTGCA GGCCCTGCTC 
TACAGCTCTG AAACCCTCAC AAGGCCCATG ACGGCTCCCC AGATATCTCG CTGTGATCAG 
GGCCACAAGG GCGGCACTAC AGCTAACCAC GCCATGTCCG GAGGGGTCAA CACCAGGGAC 
AATCTTCACC TGCTGGAGGA GGGCCAGGGC ATGCCAAGGG AAGAGCTGGA CGAGCGCATT 
GCCAGGGAGG AGTTCCGCCG TCCACGGGAG TCCCTGTTGA ACATCTGCAC CGAGTTTTAC 
AAACACTGCG GGCCACGGCT CAAAATCCTG CAGAACGTTG CAGGCGAGCC GCGGGTCACA 
GCTCTGGAGC TACTGGACAT CAAGTCCCAC ATGAGGCTGG CAGAGATCGC CCACGCCCTG 
CTGAAGCTGG CGCCCTACGA CACCCTGACC ATGGAGAGCC GAGGGCTGCG GCGTTACATC 
ATGGAGATGC TGCCCATCAC CGACTGGTCG TCCGAGGCCG TCCGGCCTGC CCTCATCCTC 
ATCCTCAAGA GGCTGGACCG CATGTTCAAC AAGATCCACA AGATGCCCAC GCTCAGGAGA 
CAGGTGGAGT GGGAGGCGGC CAGTAGCCTG ATCGAGGGGA TCTGCCTGAC CTTGCAGCGA 
CAGCCGATCA TCTCGTTCCT GCCTCACCTG CGCTCGCTTA TCAACGTCTG CGTCAACCTG 
GTGATGGGTG TGGTCGGCCC CTCCAGTGTG GCGGACGGGC TGCCACTCCT CCACCTCAGC 
CCTTACCTGT CTCCGCCTCT GCCCTTTAGC ACCGCCGTGG TCCGATTGGT TGCTCTGCAG 
ATTCAGGCCT TGAAGGAAGA CTTTCCCCTC AGTCACGTGA TCTCACCTTT CACCAATCAG 
GAAAGGCGGG AGGGGATGCT GCTCAACCTG CTCATTCCCT TCGTGTTAAC TGTAGGCTCC 
GGGAGCAAAG ACAGCCCCCA CCTGGAGCAG CCGGAGGTCT TCCTGCTCCT ACAGACAGTC 
ATCAACATCC TGCTTCCCCC TCGTATCATC TCCACATCCC GCACCAAGAA CTTTATGCTT 
GACGCCTCTC CGGCTCACTG CTCCACGCCG GGTGACCCTG GGAAAGATCT GCGTAGGGAG 
GGATTAGCAG AGTCCACGAG CCAGGCTGCT TATTTAGCTC TGAAGGTGGT GCTGGTTTGC 
TTCGAGCGCT CGCTGGGGAA CCAGTGGTAT CGGCTGAGCC TGCAGGTGAA GGAGATGGCT 
CTGAGGAAGG TGGGCGGCTT GGCCTTCTGG GACTTCATCG ACTTTATCGT CCGAACCCGG 
ATCCCTATTT TTGTCCTGCT CAGACCCTTT ATACAGTGCA AGTTGCTGAC CCAGCCGGCC 
GATTCCCAGG AAGAGATCAC AGCGCGCCAC CACATCGCTG ATCAGCTGGA GCGGCGCTTC 
ATCCCACGAC CTCTCTGCAA GAGCTCACTG TTTGCAGAGT TCAACAATGA GCTCAAGATC 
CTCAAAGAGG CTGTGCACAG CGGCTCAGCA TATCAGGGCA AGACATCCAT CAGCACGGTG 
GGCACCTCCA CGTCAGCCTA CCGCCTCAGT TTGGCCACAA TGTCGCGATC CAACACAGGC 
ACTGGAACAG TTTGGGAGCA GGACAGCCAA CCGTCACGGC AGCCCTCACA GGACACGCTC 
AGCCGTACCG ACGAGGACGA TGAAGAGATT GACTCCATGA GCATTCCCAG CGTGGTGAGC 
GAGCACGAAG CCTTCATGCC CAGGGTGATG TCACAGCGCC GTTTCTCCAG CCACGCCACT 
GGCTCGGCGG CCTCTCAGCC CGAGGCCCCC CGCACCACCA TGTTGCCCAG CCACAGTGAA 
CCCAATGTGC TAGATGAGTC CCAGGGTCTT CTGCAGGAGG GTAACCTCTC TAGGGTGGCC 
AGTGTGCAGA GCGAACCAGG TCAGAACCTC CTGATCCAGC CCCCGTTAGG AAGAAAGCGA 
GGACTCAGAC AGATGCGACG CCCCCTGCTG TCCATTCCAA AAACCGAACC ACGCGGTCGA 
TCTGGAGCGA GGCTTTCTAC AACGCGCAGG AGCATCCAGC CCAAGAACAA ACCACTGGAA 
ACTTTATTGG ACTGTGAAGC GCATGGAGAC CAAAAGAGGT CAGTCACCTT TACAGAGAAT 
CAAGGCCAGC AGGCATCCAC GGGGGCAAGC AAGCCTCCAA CTCCCAGCAC TGTGGACCCA 
CCGGGCGAAG GCAGAGGGAC GAGTCCTGCC CCATCAAAGT CCCCAACCCT GGAAGTACCC 
CCCACCTCTT CAGCCATGAT CTTGGCTGAC CTGCACGGAC CTACAAATGC ACAGGTCCCT 
CAAGCATTAA GCAGCAAGGA GAAGAGAGAG CAGTGGGGGC TACGCAGCAG CCTCTCCCCT 
CCCCCATCCA TCTCCCGGCC TTCCACTCCA ACACCCCCAT CCCGAACCTG CTCCCCTCTC 
CCCCTCTCCA GAACCTGCTC CCCTCTCCCT TTGTCCCGCA CCTCCTCTCC TCTTCCACCG 
CCACTCCCAG TGCTGAGCAC AGCACCCGCT CCGGGTCCAC CGCCGCCACC TCCATTACCA 
CCAACTCATC TTGCTGCTGC TCCTCCTCCT CCACCACCCC CGGGGCCTTC TAGGATCAGA 
GAGAGCCCCG GGGACGAGGA CACAACCGCC CTGCTGCCGC GCAGCGACAC CCTGCTGCAC 
CTCAGCGAGG ACGATGGCAC CGAGAACCCG CTTCTCACCC CGCTGCTGTC CCCTCCTCCC 
CCCCGCCGGT CGCCCCGCCG CTCTCCCCTC CCCCTCTCAC CCGTCGACCT GGACCTGGAT 
GAGTCCCACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922264.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777718.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.

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CloneID	OMa370066
Clone ID Related Accession (Same CDS sequence)	XM_012922264.3
Accession Version	XM_012922264.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922264.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GCGCATGGAG ACCAAAAGAG GTCAGTCACC 
TTTACAGAGA ATCAAGGCCA GCAGGCATCC ACGGGGGCAA GCAAGCCTCC AACTCCCAGC 
ACTGTGGACC CACCGGGCGA AGGCAGAGGG ACGAGTCCTG CCCCATCAAA GTCCCCAACC 
CTGGAAGTAC CCCCCACCTC TTCAGCCATG ATCTTGGCTG ACCTGCACGG ACCTACAAAT 
GCACAGGTCC CTCAAGCATT AAGCAGCAAG GAGAAGAGAG AGCAGTGGGG GCTACGCAGC 
AGCCTCTCCC CTCCCCCATC CATCTCCCGG CCTTCCACTC CAACACCCCC ATCCCGAACC 
TGCTCCCCTC TCCCCCTCTC CAGAACCTGC TCCCCTCTCC CTTTGTCCCG CACCTCCTCT 
CCTCTTCCAC CGCCACTCCC AGTGCTGAGC ACAGCACCCG CTCCGGGTCC ACCGCCGCCA 
CCTCCATTAC CACCAACTCA TCTTGCTGCT GCTCCTCCTC CTCCACCACC CCCGGGGCCT 
TCTAGGATCA GAGAGAGCCC CGGGGACGAG GACACAACCG CCCTGCTGCC GCGCAGCGAC 
ACCCTGCTGC ACCTCAGCGA GGACGATGGC ACCGAGAACC CGCTTCTCAC CCCGCTGCTG 
TCCCCTCCTC CCCCCCGCCG GTCGCCCCGC CGCTCTCCCC TCCCCCTCTC ACCCGTCGAC 
CTGGACCTGG ATGAGTCCCA CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777718.2
CDS	397..10623
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHSEPNVLDESQGLLQEGNLSRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922264.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922264.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGT GAACCCAATG TGCTAGATGA GTCCCAGGGT 
CTTCTGCAGG AGGGTAACCT CTCTAGGGTG GCCAGTGTGC AGAGCGAACC AGGTCAGAAC 
CTCCTGATCC AGCCCCCGTT AGGAAGAAAG CGAGGACTCA GACAGATGCG ACGCCCCCTG 
CTGTCCATTC CAAAAACCGA ACCACGCGGT CGATCTGGAG CGAGGCTTTC TACAACGCGC 
AGGAGCATCC AGCCCAAGAA CAAACCACTG GCGCATGGAG ACCAAAAGAG GTCAGTCACC 
TTTACAGAGA ATCAAGGCCA GCAGGCATCC ACGGGGGCAA GCAAGCCTCC AACTCCCAGC 
ACTGTGGACC CACCGGGCGA AGGCAGAGGG ACGAGTCCTG CCCCATCAAA GTCCCCAACC 
CTGGAAGTAC CCCCCACCTC TTCAGCCATG ATCTTGGCTG ACCTGCACGG ACCTACAAAT 
GCACAGGTCC CTCAAGCATT AAGCAGCAAG GAGAAGAGAG AGCAGTGGGG GCTACGCAGC 
AGCCTCTCCC CTCCCCCATC CATCTCCCGG CCTTCCACTC CAACACCCCC ATCCCGAACC 
TGCTCCCCTC TCCCCCTCTC CAGAACCTGC TCCCCTCTCC CTTTGTCCCG CACCTCCTCT 
CCTCTTCCAC CGCCACTCCC AGTGCTGAGC ACAGCACCCG CTCCGGGTCC ACCGCCGCCA 
CCTCCATTAC CACCAACTCA TCTTGCTGCT GCTCCTCCTC CTCCACCACC CCCGGGGCCT 
TCTAGGATCA GAGAGAGCCC CGGGGACGAG GACACAACCG CCCTGCTGCC GCGCAGCGAC 
ACCCTGCTGC ACCTCAGCGA GGACGATGGC ACCGAGAACC CGCTTCTCAC CCCGCTGCTG 
TCCCCTCCTC CCCCCCGCCG GTCGCCCCGC CGCTCTCCCC TCCCCCTCTC ACCCGTCGAC 
CTGGACCTGG ATGAGTCCCA CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc80 ( XM_012922265.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777719.2 Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.

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CloneID	OMa370067
Clone ID Related Accession (Same CDS sequence)	XM_012922265.3
Accession Version	XM_012922265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein unc-80 homolog isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922265.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAT GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777719.2
CDS	397..10566
Translation	MVKRKSLDENEPECGKGIPFPIQTFLWRQTSAFLRPKLGKQYEASCVSFERVLVENKLHGLSPALSEAIQSISRWELVQAALPHVLHCTSILLSNRNKLGHQDKLGVAETKLLHTLHWMLLEAPQECNHEPSLSHGWSRGSSSSAFLQPVGNQGSSPGAPSSCSSSGLGGLGQQHSGSLLEEDEHTRTKLFQKSMATVELFVFLFAPLVHRIKESDLTFRLASGLVIWQPMWEHRLPDIPAFTALIKPVRNIVTAKRNSPVNNQCSPCGSGNPGPTGFQVVCEATQSDSSSPSVGEQSCRRGNSVEKGGPSQQQPPPVKGPSKKKTSAPAGLPASLAQRARYATYFDVAVLRCLLQPHWTEEGVHWALMYYLQRLRQILEERPERPPEPLVAPLPRPRSSSMVAATPSLVNTHKTQDMSLKCNEEGKSLSTETFARVSLTNLRRQAVPDLSSDLGMNIFKKFKNRREDRERKGSIPFHHTGKKRQRRMGVPFLLHDDHLDVSPTRSTFSFGSFSGLGDDRRALERGGWQATIMGKFTRRGSTDTAADADSLSAKHSHSHHSLLRDMPDHSNSHSDNTVKEVRSQISTITMAAFNTTVASFNVGYADFFTEHMKKLCNPVAVPEIPVEPLACANLPRSLTDSCINYSCLEEGENIEGTNNFVLKNGMLDLTAILRALYSILSHDISSRICDVALNIIDCLLQLGVVPGMGKLSKPDNKENQEGRVKDAAAQGLGGVAQGGGPIAGAGGGGGGGGGGGGGGGSGGGSGQGSKDDVKNNKDDDKKEEAGGLSPHRLALTMLIKIVKSLGCAYGCGEGHRGLSGDRLRMQAQNCLTNLYKLDKLQFRLTMREYVNRDSLNNIVDFLHALLGFCMEPITDNKAGFGNNFATGDNKSVAQNMEAVVVGCMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKVTATKKPDEKEETKQPGPRRSEAGVSGEKGQVSSTADECRSYISSRPPQTPEHEEQIPGALLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGTTTDRRSRSRSRRISLRKKLKLPIGNWLKRSSLSGLTDGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGAVGPEYGRDEEENFFKRLGPHGFQERLAASQEAMKNKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARASLFLECARFVHRCNRGNWPEWMKGHHVNITKRGLSRGRSPIVGNKRNQKLQWNAAKHFCQWGDAIGTRLSELCHSDSESPANILGYIYDEETKRRMRKEDEEEDYLDDNTVNPTKCGCPFALKMAACQLLLEITTFLRETFPCLPRPRTEPLVDLDSCRLRLDPELGRHRYERKISFAGILDDEDGHDSLNSSSHTLKSDTTCDDKKTQEAQDNLSPLPCSPAPVRKIRIGGSRLLQIKGARSFRVKKVGSLSSIRRAGSLKSTKLSRQDSESENEEGLLSQTQSRDTVTDIGSPFSTSEPSIEPEGQSSGGTEDNYHRNMSWLHIMILLCNQQSFICTHIDFCHPRCYQHHSRSCARLVRAIKLVYGETVDSLREDSASSGNIGGRVKKNKECSDKSCLRTPSMKRRPTDSNTEGKKDTGMLKYIRNQVMSLSPAPLSLLIKAAPILTDDMYGDIQPAAWELLLSVDEHMAAAAAALFLLCAVKVPDAVTEMMMAEFQHPEACQRINSILKFYTLWRFRYQVWPRMEEGAQQIFKIPPPSINFTLPSPILGMPCVPIFDPPWVPQNTGSVQDPINEDQSKSFSARAVSRSHQRAEHILKNLQQEEEKRRLGREASIITAIPVAQEACYEPTCSPPPEQEEEAEEVVNLASRRLSVSPSCASSNSHRNYSFRRGSVWSVRSLASAEDEENTTEHTPTHHMLQPPQAVFPACICAAVLPIVHLMEDGEVREDGVAVSAVAQQILWNCLIEDPALVLRHFLEKLTVSNRQDELMYMLRKLLLNIGDLPAQTSHILFNYLVGLIMYFVRTPCEWGMDAISATLTFLWEVVGYVEGLFFKDLKQTMKKEQCEVKLLVTASMPGTKTLVVHGQNECDIPTQLPVHEDTQFEALLKECLEFFNIPEARSAHYFLMDKRWNLIHYSKTYVRDIYPFRRSVSPQLNLVHMLPEKGQELIQKQVFSRKLEEVGRVLFLISLTQHMPAVHKQSHVSLLQEDLLRLPSFPRTAIDAEFSLFNEPQGKELFGLDTLHKVLWIKLLEEMFLGMPSEYPWGDEMMLFLNVFNGALLLHPEDSSLLRQYTATAINTAVHFNHLFSLSGYQWMLPTMLQVYADYESNPLLRQGIEFCCRQFYILHRKPFILQLFASVAPLLEFTTSTSTGLSKGVSAQCLFDLLVSLEGETQDALDALELVKAEKPLRSLDFCYGNEDLTFSISEAIKLCVTVVAYAPESFRSLQMLMVLEALVPCYLQKLKSNTVTMESASAARDEIAAIAALATSLQALLYSSETLTRPMTAPQISRCDQGHKGGTTANHAMSGGVNTRDNLHLLEEGQGMPREELDERIAREEFRRPRESLLNICTEFYKHCGPRLKILQNVAGEPRVTALELLDIKSHMRLAEIAHALLKLAPYDTLTMESRGLRRYIMEMLPITDWSSEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEAASSLIEGICLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPHLEQPEVFLLLQTVINILLPPRIISTSRTKNFMLDASPAHCSTPGDPGKDLRREGLAESTSQAAYLALKVVLVCFERSLGNQWYRLSLQVKEMALRKVGGLAFWDFIDFIVRTRIPIFVLLRPFIQCKLLTQPADSQEEITARHHIADQLERRFIPRPLCKSSLFAEFNNELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSQPSRQPSQDTLSRTDEDDEEIDSMSIPSVVSEHEAFMPRVMSQRRFSSHATGSAASQPEAPRTTMLPSHRVASVQSEPGQNLLIQPPLGRKRGLRQMRRPLLSIPKTEPRGRSGARLSTTRRSIQPKNKPLAHGDQKRSVTFTENQGQQASTGASKPPTPSTVDPPGEGRGTSPAPSKSPTLEVPPTSSAMILADLHGPTNAQVPQALSSKEKREQWGLRSSLSPPPSISRPSTPTPPSRTCSPLPLSRTCSPLPLSRTSSPLPPPLPVLSTAPAPGPPPPPPLPPTHLAAAPPPPPPPGPSRIRESPGDEDTTALLPRSDTLLHLSEDDGTENPLLTPLLSPPPPRRSPRRSPLPLSPVDLDLDESHV

Target ORF information:

RefSeq Version	XM_012922265.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra unc-80 homolog, NALCN channel complex subunit (unc80), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922265.3

ORF Insert Sequence:

ATGGTGAAGA GGAAAAGCCT GGATGAAAAC GAACCGGAGT GCGGGAAGGG AATACCGTTC 
CCGATCCAGA CCTTCCTGTG GAGGCAGACC AGTGCTTTTC TGCGGCCAAA GTTGGGAAAA 
CAGTATGAGG CATCCTGTGT GTCCTTTGAG CGAGTGCTGG TGGAGAACAA GCTCCACGGG 
CTGTCGCCGG CCCTGTCAGA GGCCATCCAG AGCATCTCCC GCTGGGAGCT GGTCCAGGCG 
GCGCTGCCTC ACGTACTCCA CTGCACCTCC ATTCTGCTGT CTAACAGGAA CAAGCTTGGT 
CACCAGGACA AGTTGGGTGT GGCGGAGACC AAGCTGCTCC ACACGCTGCA CTGGATGCTG 
CTGGAGGCCC CACAGGAGTG CAACCATGAG CCCAGCCTGA GCCACGGCTG GTCCAGGGGC 
AGCAGCAGTA GCGCCTTCCT CCAGCCTGTG GGGAACCAGG GTTCCTCCCC GGGAGCTCCT 
AGCTCCTGCT CCAGCTCGGG CCTGGGCGGG TTAGGACAAC AGCACAGCGG CTCCCTGCTG 
GAGGAGGATG AGCATACTCG CACCAAACTA TTCCAAAAGA GCATGGCAAC AGTAGAGCTG 
TTTGTGTTCC TGTTTGCACC GCTTGTTCAT CGGATAAAGG AGTCAGACTT GACCTTCAGA 
CTGGCTAGTG GTCTGGTAAT ATGGCAGCCA ATGTGGGAGC ACCGCCTGCC TGACATCCCT 
GCTTTTACTG CCCTCATCAA ACCTGTCAGA AACATTGTTA CAGCAAAGAG GAACTCCCCA 
GTCAACAACC AGTGCAGCCC CTGTGGATCA GGCAACCCGG GTCCCACGGG CTTCCAGGTG 
GTTTGCGAAG CCACCCAATC AGATTCCTCC TCCCCGTCAG TCGGGGAGCA GAGTTGTCGT 
CGTGGTAACT CAGTGGAGAA AGGTGGCCCT TCCCAACAGC AACCCCCACC GGTCAAAGGC 
CCTTCCAAGA AAAAAACATC AGCTCCTGCC GGCCTGCCAG CATCGTTGGC CCAGCGGGCA 
CGTTACGCCA CTTATTTTGA TGTGGCTGTG CTGCGCTGTC TGCTGCAGCC GCACTGGACG 
GAGGAGGGCG TGCACTGGGC CTTGATGTAC TACCTGCAGC GCCTCAGGCA GATCCTGGAG 
GAGAGGCCTG AACGTCCCCC AGAGCCCTTG GTTGCACCTC TGCCACGGCC ACGCAGCAGC 
TCCATGGTGG CTGCGACACC CTCACTGGTC AACACCCACA AGACTCAGGA TATGAGCTTA 
AAATGCAATG AAGAGGGGAA ATCTCTGAGC ACAGAGACCT TTGCAAGGGT TTCCCTGACT 
AACCTTCGTC GGCAGGCTGT CCCTGACTTG TCTTCCGACC TGGGCATGAA CATCTTCAAG 
AAGTTTAAGA ACCGCCGCGA GGACCGCGAG AGAAAGGGCT CCATCCCCTT CCATCACACG 
GGGAAGAAGC GCCAGCGTCG GATGGGGGTT CCCTTCCTGC TGCACGATGA CCACCTGGAC 
GTCTCTCCCA CCCGCAGCAC CTTCTCCTTC GGAAGCTTCT CTGGCCTGGG TGACGACAGG 
CGGGCCCTGG AACGTGGAGG CTGGCAGGCC ACCATCATGG GCAAATTCAC ACGGCGGGGC 
AGCACAGACA CAGCTGCAGA TGCAGACAGC CTGAGTGCCA AACACTCTCA TTCCCACCAC 
TCGCTGCTCA GAGACATGCC CGACCACTCT AACAGCCACA GTGACAACAC CGTCAAGGAG 
GTTCGATCTC AGATCTCCAC CATCACCATG GCGGCGTTCA ACACAACAGT TGCGTCCTTT 
AACGTGGGCT ACGCCGACTT CTTCACTGAG CACATGAAGA AACTCTGCAA CCCCGTGGCT 
GTACCTGAAA TCCCAGTGGA GCCGCTGGCA TGCGCCAACC TGCCGCGCAG CCTCACTGAC 
TCCTGTATCA ACTACTCCTG TCTGGAGGAA GGCGAGAACA TCGAGGGCAC CAATAACTTT 
GTGCTGAAAA ACGGCATGTT GGACCTCACA GCTATCCTGA GAGCTCTTTA CTCCATTCTC 
AGCCACGATA TCAGTTCCAG GATCTGCGAC GTGGCTCTCA ACATCATCGA CTGCCTGCTG 
CAGCTGGGCG TGGTGCCCGG GATGGGCAAG TTGTCCAAGC CAGATAACAA GGAGAACCAA 
GAGGGTCGTG TCAAAGATGC GGCAGCTCAG GGCCTCGGAG GAGTCGCCCA GGGAGGCGGG 
CCGATTGCAG GGGCTGGAGG AGGTGGAGGA GGGGGTGGTG GAGGAGGAGG GGGAGGGGGA 
AGTGGTGGAG GGAGCGGGCA GGGGAGCAAG GACGATGTGA AAAATAATAA GGATGACGAT 
AAAAAGGAAG AAGCTGGTGG CCTCAGCCCC CACCGCCTGG CTTTGACAAT GCTCATAAAG 
ATAGTCAAGT CTCTGGGCTG TGCTTACGGC TGTGGTGAGG GCCATCGAGG ACTATCAGGA 
GATCGCCTGA GGATGCAGGC CCAGAACTGC CTCACCAACC TGTATAAGCT GGACAAGCTT 
CAGTTTCGCC TGACTATGCG CGAGTACGTC AACAGAGACT CCCTCAATAA CATAGTCGAC 
TTCCTGCATG CACTGCTGGG CTTCTGCATG GAGCCCATCA CTGACAACAA GGCGGGCTTC 
GGAAACAACT TCGCCACAGG AGACAACAAA TCTGTAGCCC AGAATATGGA GGCCGTGGTG 
GTGGGCTGCA TGTTCAAGTC CCTCATCACC CGCTGCGCCT CAACCACACA TGAGCTGCAC 
AGCCCCGAGA ACCTGGGTCT GTACTGTGAC ATCCGCCAGC TGGTCCAGTT CATTAAAGAG 
GCCCACGGAA ACGTGTTCCG CAGGGTGGCA CTGAGCGCGC TCCTGGACAG CGCGGAGAAG 
GTCACCGCCA CCAAGAAACC TGATGAGAAG GAGGAGACCA AACAGCCCGG TCCCAGGAGG 
TCAGAGGCGG GTGTGTCCGG GGAGAAGGGT CAGGTGTCCA GTACTGCAGA CGAGTGTCGC 
AGCTACATCT CCAGTAGACC CCCCCAGACA CCCGAACATG AAGAACAGAT CCCGGGTGCT 
CTGCTGGGCA GGAAGGATTT TTGGAGGAAG ATGTTCAAGT CGCAGAGCGC CGCCAGTGAC 
ACCAGCAGCC AGTCGGAACA AGACACCTCA GAGTGCACCA CCGCACACTC TGGCACCACC 
ACCGACCGCC GCTCACGATC CAGATCCCGT CGCATTTCGC TTCGCAAGAA GTTGAAGCTG 
CCTATCGGGA ACTGGTTGAA GCGCTCCTCT CTCTCTGGAC TGACTGACGG CGTCGAGGAC 
TTGCTGGATA TCAGCTCGGT GGATCGACTC TCCTTCATAC GTCAGAGTTC AAAGGTGAAG 
TTCACTAGTG CAGTGAAGCT ATCTGAAGGT GGCGCTGTAG GGCCGGAGTA CGGCAGGGAC 
GAGGAGGAGA ATTTCTTCAA GCGGCTCGGA CCTCACGGTT TCCAGGAGCG TCTCGCAGCC 
AGCCAGGAGG CCATGAAGAA CAAGAATGTA GTGAATCTGG GCGCCATCCG ACAGGGTATG 
AAACGCTTCC AGTTTCTCCT GAACTGCTGC GAGCCAGGAA CCATACCAGA TGCCTCCATC 
CTCGCCGCTG CTCTGGACCT CGAAGCCCCA GTTGTGGCCA GGGCCTCCCT TTTCTTGGAA 
TGTGCCAGAT TTGTCCACCG GTGTAATCGT GGCAACTGGC CCGAATGGAT GAAGGGCCAC 
CACGTAAACA TCACCAAGAG AGGGCTGTCC AGGGGCCGAT CGCCTATTGT GGGCAATAAA 
AGAAACCAGA AGCTCCAGTG GAACGCTGCC AAGCATTTCT GCCAGTGGGG AGACGCGATT 
GGCACGAGGC TCAGCGAACT CTGCCACTCG GACAGTGAGA GCCCCGCCAA CATCCTGGGT 
TATATTTACG ATGAAGAGAC CAAACGCAGG ATGAGGAAAG AAGACGAGGA AGAAGACTAT 
TTAGATGACA ACACGGTCAA TCCTACAAAA TGTGGCTGCC CCTTCGCACT GAAGATGGCC 
GCTTGCCAGC TGTTGTTGGA GATCACCACT TTCCTGCGAG AGACCTTCCC CTGCTTACCA 
CGACCGCGCA CCGAACCGCT TGTGGATCTG GACAGCTGCC GCCTGCGTCT GGACCCAGAG 
CTCGGCCGCC ATCGCTACGA GAGGAAGATC AGCTTTGCGG GCATCCTCGA CGACGAAGAC 
GGGCACGATT CCCTCAACAG CAGCAGCCAC ACTCTGAAGT CTGACACCAC CTGTGATGAT 
AAGAAGACCC AGGAGGCTCA GGACAATCTC TCCCCACTCC CCTGCTCTCC AGCACCAGTG 
CGGAAGATTC GTATCGGAGG CTCGCGCCTG CTTCAGATCA AAGGAGCCCG CAGTTTTCGT 
GTGAAGAAGG TTGGCTCGCT GTCTTCGATA CGCAGAGCGG GGAGTCTGAA GAGCACCAAG 
CTGTCTCGGC AGGACTCTGA GTCTGAGAAC GAAGAAGGCC TGCTGTCACA AACGCAGAGC 
AGGGACACAG TTACTGACAT CGGCAGTCCC TTCAGCACCA GTGAACCCAG CATCGAGCCA 
GAAGGTCAGA GCTCAGGAGG GACGGAGGAC AACTACCACC GCAACATGTC CTGGCTTCAC 
ATTATGATCC TGCTGTGCAA CCAGCAGAGT TTCATCTGTA CCCACATAGA CTTCTGCCAC 
CCACGCTGCT ACCAACACCA CAGCCGCTCC TGCGCCCGTC TGGTCCGTGC CATCAAGCTC 
GTGTACGGGG AGACGGTGGA CAGCCTGAGG GAGGACAGCG CCTCCTCTGG TAACATCGGA 
GGACGTGTGA AGAAAAACAA AGAGTGTTCA GACAAGTCTT GTCTGAGGAC CCCGTCCATG 
AAGAGAAGAC CCACTGACTC CAACACAGAA GGGAAGAAGG ATACAGGCAT GCTCAAGTAC 
ATCCGCAATC AGGTGATGAG TTTGTCACCA GCTCCTCTTT CACTGCTGAT CAAAGCAGCT 
CCTATCTTGA CTGACGACAT GTACGGAGAC ATCCAGCCCG CAGCCTGGGA ACTCCTACTC 
AGTGTGGATG AACATATGGC AGCCGCTGCT GCTGCCTTGT TTCTCCTGTG CGCCGTAAAG 
GTCCCTGATG CAGTGACCGA AATGATGATG GCAGAGTTCC AGCACCCTGA GGCCTGCCAG 
CGCATTAACT CCATCCTGAA GTTTTACACT CTGTGGCGTT TTCGCTACCA AGTGTGGCCT 
CGCATGGAGG AAGGAGCCCA GCAGATCTTT AAGATCCCTC CACCCAGCAT TAACTTCACT 
CTCCCTTCTC CAATACTGGG CATGCCCTGT GTCCCCATAT TTGACCCTCC CTGGGTGCCC 
CAGAACACTG GCAGCGTCCA GGATCCAATC AACGAAGACC AGTCTAAATC CTTCTCTGCG 
CGGGCGGTGT CGCGCTCCCA CCAGCGAGCC GAGCACATCC TCAAGAACCT GCAGCAGGAG 
GAGGAGAAGC GGCGTCTGGG CCGCGAGGCC AGCATCATCA CAGCCATCCC TGTGGCTCAG 
GAGGCCTGCT ACGAACCCAC ATGCAGCCCT CCACCCGAGC AAGAGGAGGA AGCAGAAGAA 
GTGGTGAACC TGGCATCACG ACGTCTGTCT GTCAGCCCAT CCTGCGCCTC CAGCAACTCC 
CACAGGAACT ACTCTTTCCG CCGGGGCTCT GTATGGTCTG TCCGCTCGCT GGCCAGTGCT 
GAAGATGAAG AAAACACAAC AGAACACACA CCCACTCACC ACATGCTACA GCCACCTCAA 
GCTGTCTTCC CAGCATGCAT CTGTGCTGCC GTCCTACCAA TAGTGCACCT CATGGAAGAC 
GGTGAAGTTA GAGAGGATGG CGTGGCTGTG AGTGCTGTTG CCCAGCAAAT CCTCTGGAAC 
TGCCTCATTG AAGATCCAGC TCTCGTTCTC CGCCACTTCC TGGAAAAGCT GACAGTTAGC 
AACAGACAGG ACGAGCTGAT GTACATGCTT AGGAAGCTCC TGCTCAACAT TGGAGATCTG 
CCAGCCCAGA CCTCTCACAT CCTTTTTAAC TACCTGGTGG GCCTGATCAT GTATTTTGTG 
CGGACTCCCT GTGAATGGGG CATGGACGCC ATCTCGGCCA CGCTGACCTT CCTCTGGGAG 
GTGGTCGGCT ACGTGGAGGG GCTCTTCTTT AAAGACCTGA AGCAGACCAT GAAGAAAGAG 
CAGTGTGAGG TCAAACTGCT GGTCACAGCA TCGATGCCAG GAACCAAAAC GCTGGTGGTG 
CACGGGCAGA ATGAATGTGA CATCCCAACC CAGCTACCAG TCCACGAAGA CACTCAGTTT 
GAAGCCCTGC TAAAGGAGTG TCTGGAATTT TTCAACATTC CCGAGGCCAG ATCGGCACAC 
TACTTCCTCA TGGATAAACG ATGGAACCTC ATCCATTATT CAAAGACATA TGTTAGAGAC 
ATCTACCCGT TCCGAAGGTC AGTCTCTCCT CAGCTCAACC TGGTCCACAT GTTGCCTGAG 
AAAGGACAGG AGCTCATCCA GAAACAGGTG TTTTCCCGTA AACTAGAAGA AGTCGGTCGA 
GTCCTCTTCC TCATCTCCCT CACACAACAC ATGCCAGCTG TGCACAAGCA GTCCCACGTC 
TCCCTGCTGC AGGAGGACCT GCTTCGCCTG CCCTCCTTCC CCCGCACCGC CATCGATGCA 
GAGTTCTCCC TCTTTAATGA GCCTCAAGGA AAAGAGCTGT TTGGCTTGGA CACTCTCCAC 
AAGGTGTTGT GGATCAAACT GTTGGAGGAA ATGTTTCTGG GCATGCCCAG TGAGTACCCG 
TGGGGCGATG AGATGATGCT GTTCCTTAAC GTCTTCAACG GGGCCCTTCT GCTGCACCCC 
GAGGACAGCT CCCTGCTGAG GCAGTACACG GCGACCGCCA TCAACACCGC CGTTCACTTT 
AACCATCTGT TCTCCCTGAG CGGGTACCAG TGGATGCTGC CAACCATGCT GCAGGTCTAT 
GCAGACTACG AGAGCAACCC TCTTCTGAGG CAGGGCATTG AGTTTTGCTG CCGGCAGTTT 
TACATCCTCC ATCGAAAACC CTTTATCCTG CAGCTGTTTG CCAGCGTTGC TCCGCTGCTC 
GAGTTCACAA CCAGCACCAG TACCGGTCTT TCTAAAGGAG TTTCGGCCCA GTGTCTGTTC 
GACCTGCTGG TCTCTCTAGA AGGTGAAACC CAGGATGCCC TGGATGCTCT GGAGCTGGTA 
AAAGCTGAGA AGCCTCTACG CTCTCTGGAT TTCTGTTATG GTAATGAGGA CCTGACCTTT 
TCTATCAGCG AGGCCATCAA GCTGTGCGTC ACTGTGGTTG CATATGCCCC CGAATCCTTC 
AGGAGTCTCC AGATGCTGAT GGTGCTGGAG GCCTTGGTTC CCTGCTACCT CCAGAAACTG 
AAGAGCAACA CAGTTACGAT GGAGTCTGCT TCGGCTGCCA GAGATGAGAT TGCGGCCATC 
GCTGCCCTAG CTACGTCTCT GCAGGCCCTG CTCTACAGCT CTGAAACCCT CACAAGGCCC 
ATGACGGCTC CCCAGATATC TCGCTGTGAT CAGGGCCACA AGGGCGGCAC TACAGCTAAC 
CACGCCATGT CCGGAGGGGT CAACACCAGG GACAATCTTC ACCTGCTGGA GGAGGGCCAG 
GGCATGCCAA GGGAAGAGCT GGACGAGCGC ATTGCCAGGG AGGAGTTCCG CCGTCCACGG 
GAGTCCCTGT TGAACATCTG CACCGAGTTT TACAAACACT GCGGGCCACG GCTCAAAATC 
CTGCAGAACG TTGCAGGCGA GCCGCGGGTC ACAGCTCTGG AGCTACTGGA CATCAAGTCC 
CACATGAGGC TGGCAGAGAT CGCCCACGCC CTGCTGAAGC TGGCGCCCTA CGACACCCTG 
ACCATGGAGA GCCGAGGGCT GCGGCGTTAC ATCATGGAGA TGCTGCCCAT CACCGACTGG 
TCGTCCGAGG CCGTCCGGCC TGCCCTCATC CTCATCCTCA AGAGGCTGGA CCGCATGTTC 
AACAAGATCC ACAAGATGCC CACGCTCAGG AGACAGGTGG AGTGGGAGGC GGCCAGTAGC 
CTGATCGAGG GGATCTGCCT GACCTTGCAG CGACAGCCGA TCATCTCGTT CCTGCCTCAC 
CTGCGCTCGC TTATCAACGT CTGCGTCAAC CTGGTGATGG GTGTGGTCGG CCCCTCCAGT 
GTGGCGGACG GGCTGCCACT CCTCCACCTC AGCCCTTACC TGTCTCCGCC TCTGCCCTTT 
AGCACCGCCG TGGTCCGATT GGTTGCTCTG CAGATTCAGG CCTTGAAGGA AGACTTTCCC 
CTCAGTCACG TGATCTCACC TTTCACCAAT CAGGAAAGGC GGGAGGGGAT GCTGCTCAAC 
CTGCTCATTC CCTTCGTGTT AACTGTAGGC TCCGGGAGCA AAGACAGCCC CCACCTGGAG 
CAGCCGGAGG TCTTCCTGCT CCTACAGACA GTCATCAACA TCCTGCTTCC CCCTCGTATC 
ATCTCCACAT CCCGCACCAA GAACTTTATG CTTGACGCCT CTCCGGCTCA CTGCTCCACG 
CCGGGTGACC CTGGGAAAGA TCTGCGTAGG GAGGGATTAG CAGAGTCCAC GAGCCAGGCT 
GCTTATTTAG CTCTGAAGGT GGTGCTGGTT TGCTTCGAGC GCTCGCTGGG GAACCAGTGG 
TATCGGCTGA GCCTGCAGGT GAAGGAGATG GCTCTGAGGA AGGTGGGCGG CTTGGCCTTC 
TGGGACTTCA TCGACTTTAT CGTCCGAACC CGGATCCCTA TTTTTGTCCT GCTCAGACCC 
TTTATACAGT GCAAGTTGCT GACCCAGCCG GCCGATTCCC AGGAAGAGAT CACAGCGCGC 
CACCACATCG CTGATCAGCT GGAGCGGCGC TTCATCCCAC GACCTCTCTG CAAGAGCTCA 
CTGTTTGCAG AGTTCAACAA TGAGCTCAAG ATCCTCAAAG AGGCTGTGCA CAGCGGCTCA 
GCATATCAGG GCAAGACATC CATCAGCACG GTGGGCACCT CCACGTCAGC CTACCGCCTC 
AGTTTGGCCA CAATGTCGCG ATCCAACACA GGCACTGGAA CAGTTTGGGA GCAGGACAGC 
CAACCGTCAC GGCAGCCCTC ACAGGACACG CTCAGCCGTA CCGACGAGGA CGATGAAGAG 
ATTGACTCCA TGAGCATTCC CAGCGTGGTG AGCGAGCACG AAGCCTTCAT GCCCAGGGTG 
ATGTCACAGC GCCGTTTCTC CAGCCACGCC ACTGGCTCGG CGGCCTCTCA GCCCGAGGCC 
CCCCGCACCA CCATGTTGCC CAGCCACAGG GTGGCCAGTG TGCAGAGCGA ACCAGGTCAG 
AACCTCCTGA TCCAGCCCCC GTTAGGAAGA AAGCGAGGAC TCAGACAGAT GCGACGCCCC 
CTGCTGTCCA TTCCAAAAAC CGAACCACGC GGTCGATCTG GAGCGAGGCT TTCTACAACG 
CGCAGGAGCA TCCAGCCCAA GAACAAACCA CTGGCGCATG GAGACCAAAA GAGGTCAGTC 
ACCTTTACAG AGAATCAAGG CCAGCAGGCA TCCACGGGGG CAAGCAAGCC TCCAACTCCC 
AGCACTGTGG ACCCACCGGG CGAAGGCAGA GGGACGAGTC CTGCCCCATC AAAGTCCCCA 
ACCCTGGAAG TACCCCCCAC CTCTTCAGCC ATGATCTTGG CTGACCTGCA CGGACCTACA 
AATGCACAGG TCCCTCAAGC ATTAAGCAGC AAGGAGAAGA GAGAGCAGTG GGGGCTACGC 
AGCAGCCTCT CCCCTCCCCC ATCCATCTCC CGGCCTTCCA CTCCAACACC CCCATCCCGA 
ACCTGCTCCC CTCTCCCCCT CTCCAGAACC TGCTCCCCTC TCCCTTTGTC CCGCACCTCC 
TCTCCTCTTC CACCGCCACT CCCAGTGCTG AGCACAGCAC CCGCTCCGGG TCCACCGCCG 
CCACCTCCAT TACCACCAAC TCATCTTGCT GCTGCTCCTC CTCCTCCACC ACCCCCGGGG 
CCTTCTAGGA TCAGAGAGAG CCCCGGGGAC GAGGACACAA CCGCCCTGCT GCCGCGCAGC 
GACACCCTGC TGCACCTCAG CGAGGACGAT GGCACCGAGA ACCCGCTTCT CACCCCGCTG 
CTGTCCCCTC CTCCCCCCCG CCGGTCGCCC CGCCGCTCTC CCCTCCCCCT CTCACCCGTC 
GACCTGGACC TGGATGAGTC CCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	unc80
Entrez Gene ID	101470347
Full Name	unc-80 homolog, NALCN channel complex subunit
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

unc80 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: