eea1 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	eea1
Entrez Gene ID	101467934
Full Name	early endosome antigen 1
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012922509.1	XP_012777963.1	early endosome antigen 1 isoform X2
XM_012922508.1	XP_012777962.1	early endosome antigen 1 isoform X1
XM_012922509.3	XP_012777963.1	early endosome antigen 1 isoform X2
XM_012922508.3	XP_012777962.1	early endosome antigen 1 isoform X1
XM_012922508.2	XP_012777962.1	early endosome antigen 1 isoform X1
XM_012922509.2	XP_012777963.1	early endosome antigen 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following eea1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eea1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa08330	XM_012922508.3 Latest version!	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa08331	XM_012922509.3 Latest version!	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMa08331	XM_012922509.1	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa08330	XM_012922508.1	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa08330	XM_012922508.2	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa08331	XM_012922509.2	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eea1 ( XM_012922508.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777962.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa08330
Clone ID Related Accession (Same CDS sequence)	XM_012922508.1 , XM_012922508.3 , XM_012922508.2
Accession Version	XM_012922508.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	early endosome antigen 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445207.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777962.1
CDS	107..4309
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEIRKLEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922508.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922508.1

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012922508.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777962.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa08330
Clone ID Related Accession (Same CDS sequence)	XM_012922508.1 , XM_012922508.3 , XM_012922508.2
Accession Version	XM_012922508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777962.1
CDS	111..4313
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEIRKLEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922508.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922508.3

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012922508.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777962.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

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CloneID	OMa08330
Clone ID Related Accession (Same CDS sequence)	XM_012922508.1 , XM_012922508.3 , XM_012922508.2
Accession Version	XM_012922508.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	early endosome antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445207.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012922508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777962.1
CDS	111..4313
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEIRKLEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922508.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922508.2

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATTCGTAAAC TGGAGATGAC CATATCCCAA 
CTGAAGGCAG AGCTGGAGAA GGGTCCTCAG GAAGCAGCCG TCTACACGCA ACAGATTCAT 
GAGTTGCAGA GCAATTTGAA TAACTTGCAG CAGCAAAGTC AGAGCCTGTC TGAAAAGCTT 
GCACGCAAGG AGAAGGAGTA TCAAGATCTG GAGGAGCATC TAGGGGCTGA GAAAGCTTCC 
AAGAAGGAAG TCCAAGAAAG CCTGAGGGAA AGGGAATTAG AAGTGCAAGA GCTGCAGGCT 
CGGGCCACAG GGGCTGAGGC GTCCCTGCAC AAAGCCCAGA TGGAGGTCTC AGAGAGGGCT 
GAGGAGGTGG CAAAGTTGAA GAGTGAGATT ACAGAGCTAG AGGTGAAGCA TGCAGAGTTA 
AAGGTTGAGA GGAAACAGCT GGAACAGCAG CGGGAAGAAA AGGAAAGCCA AGGTGCTCAG 
CAGCAGACTG AGATCAGTCA GCTGCATGCC AAACTGTTGG AGGCAGAGAG GCAGCTGGGT 
GAGGTGCAAG GTCGATTGAA AGAACAGAGG CAGCTGTCGG GGGAGAAACT CAAGGACCGC 
GAGCAACAAG CAGCCGACCT GCAGCTCAAA CTCTCCCGTG CAGAGGAACA GTTGAAGGAG 
AGTGCCAGTA AGAGCACAGA CCTGCAGCAC CAGCTGGAGA AAGCCAAGCA GCAGCACCAG 
GAGCTGCAGG CACTGCAGCA AAACACCAAC GGCAAACTCC GCGAGGCGCA GAATGACCTG 
GAGCAGGTGT TGCGTCAAAT CGGAGATAAA GACCAGAAGA TACAGAACCT GGAAGCTCTG 
CTTCAGAAGA GTAAAGACAC AGTGAGCCAG CTGGAAGCTG AGAGAGAGGA CCTGTGTGCC 
AAAATCCAGG CCGGGGAAGG AGAGACAGCT GTCCTTAACC AGCTTAAAGA GAAAAACCAT 
GCACTACAAG AACAAGTAAC GCAGTTGACA GACAAGCTCA AGAACCAATC AGAGAGCAAC 
AAGCAAGCAC AAGATAATCT ACACAAGCAG GTCCAGGAGC AGAAGACTCT GCTCCGCTCG 
GCCCAGGACC GAGCTCATAC CCTGGAGACT TCAGTCACTG AACTCACCAC CCAGCTCACG 
GAGAGCAAGG AGAAAGTGGC CCAGTTGGAT ACTCAGCTGA AGGCAAAGAC AGAGTTGTTG 
CTCTCTGCAG AGGCAGCTAA GGCTGCACAG AAAGCAAACC TCGAGAACAG CCTGGAGTCT 
GCCCAACATG CACTACAGGA CAAGCAGCAG GAGCTGAATA AGGTTCAGAA GAACCTGGAG 
GATCATGGCC AAAGGCTCAA AGAGAGACAA GAGCAGTGCA CACAACTAGA AAGCAGTCTG 
AAGGAATGCA AAGACAAGCT ACTGGCCTCA GAGCAGCGGA TAGAGCAGCT GGAGGGACTC 
GGCAAGAAAT TGGAGTCACA GGTAGGGGAG ATGCAGGCTG CACGGGATCA GGCGCAGCAG 
GAACTGCAGC AGCTGCAGAA GGAAAGTTCG GAAGTTAAAA AGAAAGCCAA GGAGCTACAA 
CGCTCGCTGG AGACTGAGAA AGCAGGTGCT ACAAGTCTGC AGGAGGAATT AAAGAAAAAG 
GCAGGTACCT TGAGTGATGT CCAACAGAAG CTAGCACAAT CCGAGCAGGG GATTGCTACT 
TTGAAGGCAA ACCTGGAAAA GGTGACCCAG GAGGGAAAGT CTCAGCATGC AGAGCTGGAT 
AAAAAAGCTC AGAGTCTGGC AGCAGAGTTA CAAAAGGCCC AAGAGGGAAA AAAGGCTCAA 
CAAAAGGAGC TTGCAGCTAC TCAGGAGAGT CTCGGAAAGG CTAACAAGGC ACTGAAAGAG 
AGTCAGAGTC AACTGGACAC TGAGAGGAAG AACCATAAAT CAGCTATAGA GGAGAAGGAT 
AAGTCTAATG AAAAAGCAAG ACAGGAGCTT CTGAAAAATA ATGAGTCCAT CAGAAAGGCA 
ATGAAGGAAT CCAAAGAACA GCTGGAGCAG ATGAGAGAGT CAGAGAAGCA GCTGAAAGTG 
CAGCTGACCT CATTAGAACA GCAGCACACA AAGACTCGGG AGACACTGAG GGAAAAGGAG 
AACGAGCTGG AAAAACTGCG GACGCAACTG AAGATGACCC AGGGATCTTT GGAAGAAGAG 
ATTAAGAAAC TGAATGGCCA AGTGACAGAG CTAAAGGAAG CTGGTGTCAA GAAAGCACAA 
GAAGAAAATA AGCTGAGGGC ACAGGTGTCA GGGCTCGGCC AGGAGCTGAC CTCCGAGAAG 
AGCCGGACAG CAGAGCTGCA GAAGGCCTTG GAGCAAAGCA AGGAAAACAT CAGTAAGCTT 
CAGTCTGACG TGTACGGAAA AGACTCTGAG GTTTCTGCCC TCCGTCAGGA TCTCAAGGCG 
TCTGAGCAGA AACTGAGCGT GGCTCAGGAG GAGCTGGCTG CTAATCAAAC CCATCAGTCA 
GGCTTAGAGT CCCAGATCCA GGACTTGAAA TCAGCCCGTA GCTCGCTGGA GCAGGAGCTT 
GCCAAACGGG ACGAGAAGCT CCAACAGAAG GAGCAAACCC TGAAGGAGTT GCAGAAGCAA 
AAGGGTCAAG TTAAGGAGGA GCTGGACAAG GAGAAGAGCA ATGTGGAGGA GCTGAACAAA 
GCAAAGAGTG TCCTGGAGAA GAACAACAGC AGGCTGACTT CAGAGTTAAA AACGCTAACA 
GAGAAGAGTG AGAAGGAGCT CGGCGAATTG CAGGAAGCCA AACAGCTGTT GATCCAGCAG 
AAACTTGAGC TGCAGGCTCA GGTGGAGGCA GCACAGGGTG CTCTCAAGCA GGAGCAGAAG 
GAGCACCAGA CCACCAAGGA CGGCAGGAGT GAGAGGGAGG AGCAGCTCCT AGCTCAAATC 
AGGGATGCTC AGGATCAGCT GGCAGCAGAG AAGAAGAGAT GGCAAGAGCA GGTGAAACGT 
GGGGAGGAGG CAGAAGCTAA GATGGGTTTG CAGGTGACAG CCCTGAATGA AAACGTGGCC 
ACGTTGAAGA GGGAATGGCA GGGCAGCCAG CGGAGAGTCG GCGAACTCGA GAAACAGACG 
GATGAGCTGA GAGGAGAAAT CGCTGTGCTG GAGGCCACCG TGCAGAACAA CCAGGACGAG 
CGACGGGCTC TTTTGGAAAG GTGCGTGAAG GGTGAAGGTG AGATCGAGAA GCTCCAGGCC 
AAAGTGGTGG AGCTAAGGAG GAAACTGGAT GACACGACGG CTGCCATGCA GGAGCTCGGC 
AGAGAGAACC AGTCGCTTCA GATCAAACAG TCACAGAGTC TGACCAGAAA GTGGGCTGAG 
GATCATGAAG TGCAGAACTG CATGGCCTGT GGGAAAGGCT TCACCGTCAC AGTCAGGAAG 
CACCACTGCA GACATTGTGG AAACATCTTC TGTGCCGAGT GTTCGTCAAG GAACGCCCTC 
ACTCCATCCT CTAAAAAGCC AGTACGCGTG TGTGAGACAT GCTTCGAGGA GCTCCAAGGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012922509.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777963.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

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CloneID	OMa08331
Clone ID Related Accession (Same CDS sequence)	XM_012922509.1 , XM_012922509.3 , XM_012922509.2
Accession Version	XM_012922509.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	early endosome antigen 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445207.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777963.1
CDS	107..4294
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922509.1
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922509.1

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012922509.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777963.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa08331
Clone ID Related Accession (Same CDS sequence)	XM_012922509.1 , XM_012922509.3 , XM_012922509.2
Accession Version	XM_012922509.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012922509.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777963.1
CDS	111..4298
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922509.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922509.3

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eea1 ( XM_012922509.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012777963.1 Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa08331
Clone ID Related Accession (Same CDS sequence)	XM_012922509.1 , XM_012922509.3 , XM_012922509.2
Accession Version	XM_012922509.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	early endosome antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014445207.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012922509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012777963.1
CDS	111..4298
Translation	MLRRILQMTPGKGGSQNAESEQPSTDLNHDQTSEGFICPQCMKSHNSAEELFKHYELFHDTGDLPAHVAPTREDLTMLRQEVQDLHTSLKEERWFSGELKKELDKVQGHLKQNDGQMSADDSVLERKLNEAETEKFNIKQMKDLFEQKAAQLATEIVDLKSRYDEEKSLREAADQRLAKLNEQLQEAKEENERLQTELLQRPGVEDVEVLQKELVQVQTLMDNMTREREEESERLKNHYEQLQANYTTSEMTISQLKAELEKGPQEAAVYTQQIHELQSNLNNLQQQSQSLSEKLARKEKEYQDLEEHLGAEKASKKEVQESLRERELEVQELQARATGAEASLHKAQMEVSERAEEVAKLKSEITELEVKHAELKVERKQLEQQREEKESQGAQQQTEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQAADLQLKLSRAEEQLKESASKSTDLQHQLEKAKQQHQELQALQQNTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKDTVSQLEAEREDLCAKIQAGEGETAVLNQLKEKNHALQEQVTQLTDKLKNQSESNKQAQDNLHKQVQEQKTLLRSAQDRAHTLETSVTELTTQLTESKEKVAQLDTQLKAKTELLLSAEAAKAAQKANLENSLESAQHALQDKQQELNKVQKNLEDHGQRLKERQEQCTQLESSLKECKDKLLASEQRIEQLEGLGKKLESQVGEMQAARDQAQQELQQLQKESSEVKKKAKELQRSLETEKAGATSLQEELKKKAGTLSDVQQKLAQSEQGIATLKANLEKVTQEGKSQHAELDKKAQSLAAELQKAQEGKKAQQKELAATQESLGKANKALKESQSQLDTERKNHKSAIEEKDKSNEKARQELLKNNESIRKAMKESKEQLEQMRESEKQLKVQLTSLEQQHTKTRETLREKENELEKLRTQLKMTQGSLEEEIKKLNGQVTELKEAGVKKAQEENKLRAQVSGLGQELTSEKSRTAELQKALEQSKENISKLQSDVYGKDSEVSALRQDLKASEQKLSVAQEELAANQTHQSGLESQIQDLKSARSSLEQELAKRDEKLQQKEQTLKELQKQKGQVKEELDKEKSNVEELNKAKSVLEKNNSRLTSELKTLTEKSEKELGELQEAKQLLIQQKLELQAQVEAAQGALKQEQKEHQTTKDGRSEREEQLLAQIRDAQDQLAAEKKRWQEQVKRGEEAEAKMGLQVTALNENVATLKREWQGSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVELRRKLDDTTAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFTVTVRKHHCRHCGNIFCAECSSRNALTPSSKKPVRVCETCFEELQG

Target ORF information:

RefSeq Version	XM_012922509.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra early endosome antigen 1 (eea1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012922509.2

ORF Insert Sequence:

ATGCTCAGAC GGATACTGCA AATGACTCCC GGGAAGGGAG GGTCTCAGAA TGCAGAGTCA 
GAGCAGCCCA GCACCGACCT TAACCACGAT CAGACATCTG AGGGCTTCAT CTGTCCTCAG 
TGCATGAAGT CTCACAACTC TGCAGAGGAG CTGTTCAAGC ACTATGAGCT GTTCCATGAT 
ACCGGAGACC TCCCTGCTCA CGTGGCCCCC ACTAGGGAAG ACCTCACAAT GCTGCGGCAG 
GAGGTCCAGG ACCTGCACAC TTCTCTCAAG GAGGAAAGAT GGTTCTCTGG TGAGCTAAAA 
AAGGAGTTAG ACAAAGTCCA AGGACACCTG AAGCAGAACG ATGGGCAGAT GAGCGCGGAT 
GATTCAGTAC TTGAAAGGAA GCTGAATGAA GCTGAGACAG AGAAGTTCAA CATCAAACAG 
ATGAAAGACC TGTTTGAGCA GAAGGCTGCC CAGCTGGCCA CAGAGATAGT AGATTTGAAG 
TCCCGTTACG ATGAGGAGAA AAGCCTCAGG GAGGCTGCTG ACCAGAGATT AGCCAAGTTG 
AACGAACAAC TGCAGGAAGC GAAGGAGGAG AACGAGCGAC TCCAAACAGA GCTGCTGCAG 
CGACCGGGCG TTGAGGATGT CGAGGTCCTG CAGAAGGAGC TGGTCCAGGT GCAAACACTG 
ATGGACAACA TGACCCGTGA GAGGGAGGAA GAGTCAGAGC GCCTCAAAAA CCATTATGAG 
CAACTGCAGG CTAATTACAC TACCTCAGAG ATGACCATAT CCCAACTGAA GGCAGAGCTG 
GAGAAGGGTC CTCAGGAAGC AGCCGTCTAC ACGCAACAGA TTCATGAGTT GCAGAGCAAT 
TTGAATAACT TGCAGCAGCA AAGTCAGAGC CTGTCTGAAA AGCTTGCACG CAAGGAGAAG 
GAGTATCAAG ATCTGGAGGA GCATCTAGGG GCTGAGAAAG CTTCCAAGAA GGAAGTCCAA 
GAAAGCCTGA GGGAAAGGGA ATTAGAAGTG CAAGAGCTGC AGGCTCGGGC CACAGGGGCT 
GAGGCGTCCC TGCACAAAGC CCAGATGGAG GTCTCAGAGA GGGCTGAGGA GGTGGCAAAG 
TTGAAGAGTG AGATTACAGA GCTAGAGGTG AAGCATGCAG AGTTAAAGGT TGAGAGGAAA 
CAGCTGGAAC AGCAGCGGGA AGAAAAGGAA AGCCAAGGTG CTCAGCAGCA GACTGAGATC 
AGTCAGCTGC ATGCCAAACT GTTGGAGGCA GAGAGGCAGC TGGGTGAGGT GCAAGGTCGA 
TTGAAAGAAC AGAGGCAGCT GTCGGGGGAG AAACTCAAGG ACCGCGAGCA ACAAGCAGCC 
GACCTGCAGC TCAAACTCTC CCGTGCAGAG GAACAGTTGA AGGAGAGTGC CAGTAAGAGC 
ACAGACCTGC AGCACCAGCT GGAGAAAGCC AAGCAGCAGC ACCAGGAGCT GCAGGCACTG 
CAGCAAAACA CCAACGGCAA ACTCCGCGAG GCGCAGAATG ACCTGGAGCA GGTGTTGCGT 
CAAATCGGAG ATAAAGACCA GAAGATACAG AACCTGGAAG CTCTGCTTCA GAAGAGTAAA 
GACACAGTGA GCCAGCTGGA AGCTGAGAGA GAGGACCTGT GTGCCAAAAT CCAGGCCGGG 
GAAGGAGAGA CAGCTGTCCT TAACCAGCTT AAAGAGAAAA ACCATGCACT ACAAGAACAA 
GTAACGCAGT TGACAGACAA GCTCAAGAAC CAATCAGAGA GCAACAAGCA AGCACAAGAT 
AATCTACACA AGCAGGTCCA GGAGCAGAAG ACTCTGCTCC GCTCGGCCCA GGACCGAGCT 
CATACCCTGG AGACTTCAGT CACTGAACTC ACCACCCAGC TCACGGAGAG CAAGGAGAAA 
GTGGCCCAGT TGGATACTCA GCTGAAGGCA AAGACAGAGT TGTTGCTCTC TGCAGAGGCA 
GCTAAGGCTG CACAGAAAGC AAACCTCGAG AACAGCCTGG AGTCTGCCCA ACATGCACTA 
CAGGACAAGC AGCAGGAGCT GAATAAGGTT CAGAAGAACC TGGAGGATCA TGGCCAAAGG 
CTCAAAGAGA GACAAGAGCA GTGCACACAA CTAGAAAGCA GTCTGAAGGA ATGCAAAGAC 
AAGCTACTGG CCTCAGAGCA GCGGATAGAG CAGCTGGAGG GACTCGGCAA GAAATTGGAG 
TCACAGGTAG GGGAGATGCA GGCTGCACGG GATCAGGCGC AGCAGGAACT GCAGCAGCTG 
CAGAAGGAAA GTTCGGAAGT TAAAAAGAAA GCCAAGGAGC TACAACGCTC GCTGGAGACT 
GAGAAAGCAG GTGCTACAAG TCTGCAGGAG GAATTAAAGA AAAAGGCAGG TACCTTGAGT 
GATGTCCAAC AGAAGCTAGC ACAATCCGAG CAGGGGATTG CTACTTTGAA GGCAAACCTG 
GAAAAGGTGA CCCAGGAGGG AAAGTCTCAG CATGCAGAGC TGGATAAAAA AGCTCAGAGT 
CTGGCAGCAG AGTTACAAAA GGCCCAAGAG GGAAAAAAGG CTCAACAAAA GGAGCTTGCA 
GCTACTCAGG AGAGTCTCGG AAAGGCTAAC AAGGCACTGA AAGAGAGTCA GAGTCAACTG 
GACACTGAGA GGAAGAACCA TAAATCAGCT ATAGAGGAGA AGGATAAGTC TAATGAAAAA 
GCAAGACAGG AGCTTCTGAA AAATAATGAG TCCATCAGAA AGGCAATGAA GGAATCCAAA 
GAACAGCTGG AGCAGATGAG AGAGTCAGAG AAGCAGCTGA AAGTGCAGCT GACCTCATTA 
GAACAGCAGC ACACAAAGAC TCGGGAGACA CTGAGGGAAA AGGAGAACGA GCTGGAAAAA 
CTGCGGACGC AACTGAAGAT GACCCAGGGA TCTTTGGAAG AAGAGATTAA GAAACTGAAT 
GGCCAAGTGA CAGAGCTAAA GGAAGCTGGT GTCAAGAAAG CACAAGAAGA AAATAAGCTG 
AGGGCACAGG TGTCAGGGCT CGGCCAGGAG CTGACCTCCG AGAAGAGCCG GACAGCAGAG 
CTGCAGAAGG CCTTGGAGCA AAGCAAGGAA AACATCAGTA AGCTTCAGTC TGACGTGTAC 
GGAAAAGACT CTGAGGTTTC TGCCCTCCGT CAGGATCTCA AGGCGTCTGA GCAGAAACTG 
AGCGTGGCTC AGGAGGAGCT GGCTGCTAAT CAAACCCATC AGTCAGGCTT AGAGTCCCAG 
ATCCAGGACT TGAAATCAGC CCGTAGCTCG CTGGAGCAGG AGCTTGCCAA ACGGGACGAG 
AAGCTCCAAC AGAAGGAGCA AACCCTGAAG GAGTTGCAGA AGCAAAAGGG TCAAGTTAAG 
GAGGAGCTGG ACAAGGAGAA GAGCAATGTG GAGGAGCTGA ACAAAGCAAA GAGTGTCCTG 
GAGAAGAACA ACAGCAGGCT GACTTCAGAG TTAAAAACGC TAACAGAGAA GAGTGAGAAG 
GAGCTCGGCG AATTGCAGGA AGCCAAACAG CTGTTGATCC AGCAGAAACT TGAGCTGCAG 
GCTCAGGTGG AGGCAGCACA GGGTGCTCTC AAGCAGGAGC AGAAGGAGCA CCAGACCACC 
AAGGACGGCA GGAGTGAGAG GGAGGAGCAG CTCCTAGCTC AAATCAGGGA TGCTCAGGAT 
CAGCTGGCAG CAGAGAAGAA GAGATGGCAA GAGCAGGTGA AACGTGGGGA GGAGGCAGAA 
GCTAAGATGG GTTTGCAGGT GACAGCCCTG AATGAAAACG TGGCCACGTT GAAGAGGGAA 
TGGCAGGGCA GCCAGCGGAG AGTCGGCGAA CTCGAGAAAC AGACGGATGA GCTGAGAGGA 
GAAATCGCTG TGCTGGAGGC CACCGTGCAG AACAACCAGG ACGAGCGACG GGCTCTTTTG 
GAAAGGTGCG TGAAGGGTGA AGGTGAGATC GAGAAGCTCC AGGCCAAAGT GGTGGAGCTA 
AGGAGGAAAC TGGATGACAC GACGGCTGCC ATGCAGGAGC TCGGCAGAGA GAACCAGTCG 
CTTCAGATCA AACAGTCACA GAGTCTGACC AGAAAGTGGG CTGAGGATCA TGAAGTGCAG 
AACTGCATGG CCTGTGGGAA AGGCTTCACC GTCACAGTCA GGAAGCACCA CTGCAGACAT 
TGTGGAAACA TCTTCTGTGC CGAGTGTTCG TCAAGGAACG CCCTCACTCC ATCCTCTAAA 
AAGCCAGTAC GCGTGTGTGA GACATGCTTC GAGGAGCTCC AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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eea1 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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