rusc2 gene cDNA ORF clone, Maylandia zebra(zebra mbuna)

mRNA	Protein	Name
XM_004555658.4	XP_004555715.2	iporin isoform X2
XM_004555659.2	XP_004555716.1	iporin isoform X3
XM_004555659.4	XP_004555716.2	iporin isoform X3
XM_012920345.4	XP_012775799.2	iporin isoform X4
XM_004555657.3	XP_004555714.1	iporin isoform X1
XM_004555657.4	XP_004555714.2	iporin isoform X1
XM_004555661.3	XP_004555718.1	iporin isoform X5
XM_023152555.1	XP_023008323.1	iporin isoform X5
XM_023152555.2	XP_023008323.2	iporin isoform X5
XM_004555657.2	XP_004555714.1	iporin isoform X1
XM_004555658.2	XP_004555715.1	iporin isoform X2
XM_004555659.3	XP_004555716.1	iporin isoform X3
XM_004555661.5	XP_004555718.2	iporin isoform X6
XM_004555658.3	XP_004555715.1	iporin isoform X2
XM_004555661.4	XP_004555718.1	iporin isoform X6
XM_012920345.2	XP_012775799.1	iporin isoform X4
XM_012920345.3	XP_012775799.1	iporin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa312396	XM_023152555.1 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34673	XM_004555657.3 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34674	XM_004555658.3 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34675	XM_004555659.3 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34676	XM_012920345.3 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34677	XM_004555661.4 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361235	XM_004555657.4 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361236	XM_004555658.4 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361237	XM_004555659.4 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361238	XM_012920345.4 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361239	XM_023152555.2 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa361240	XM_004555661.5 Latest version!	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMa34673	XM_004555657.2	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34675	XM_004555659.2	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34674	XM_004555658.2	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34677	XM_004555661.3	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMa34676	XM_012920345.2	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

rusc2 ( XM_023152555.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008323.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMa312396
Clone ID Related Accession (Same CDS sequence)	XM_023152555.1
Accession Version	XM_023152555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCAGTGGGCC AATCAGCTAA GCTGCAGCAG 
AGCTACAGCG ACTTCCTGTC CGACTACTTT TCCCTGACAG AGAAGCCGCC TGAGGAGTTC 
TGCCTCTCCC CGGACGCCTC CACCTCCTCC TCTTTGTGCT CGTCCTCGCA GTCTCACATC 
TCTGTTGACC TGACGCAGAA GAGAGCTCTG GTGAAAGCCG TGAACACTGC AGTGGATCTG 
ATCGTGGCTC ACTTCGGCAC CAGCAGAGAC CCCGATGTTA AGGCAAAGCT CGGGAACAGC 
TGGGTGAGTC CCAACGTGGG TCACCTCATC CTGAAGTATC TGTGTCCGGC TCTACGTGAC 
GTGCTGCAGG ACGGTCTGAA GGCCTACGTG CTGGACCTGA TCATTGGACA GCGCCGCTGT 
CAGCCCTGGA GCCTCGTGGA GGCGTCCACA CAGCTGGGCC CGTCCACACG TGTTCTCCAC 
AGCCTGCTCT CAAAGGTGAG CCAGTACTCG GAGCTCACGA GCCACAGCAT GAGACTGAAT 
GCTTTCATCT TCGGCCTGCT CAATCTGAAA TCTTTGGAAT TCTGGTTCAA TCACCTCTAC 
ACGCATGAAG ATATTATTGC CGCATACTAC AGCCCTTGGG GTTTCCTTCC CCTGTCACAG 
GGGCCGTGTC AGCCCCTCTT TGAGGAGCTC CTCCTCCTGC TGCAGCCTCT CTCACTGCTC 
CCCTTTGACC TGGACCTGCT GTTTGAACCA CATCTCCTCC AAAAGGGACA AGAGCAGCTT 
CGTCGCAAAG AGCAGCTCTG CTCTGCAGGC CAGAGCGTCG ACCAGTCGAA GTGCTCCACC 
TTCCAGCTCA TGAGGGGGTG GAGCAACACT GTCAGTGAAA TGGTCAAAGA CTCAAGCGCA 
GAGGTGAAGA AAGAGAAGGC GGGGCTCAGG CGAGAGGGAA CATGGCCAAG GATGGAGGGA 
GTTGGATCGA GAAGAGAGGC AGCAGCGGGG AAATCGATGC TGAGGAAAGA GAGCGTTACA 
GCTCAGAGCA TGACAGCTGG GCCTGATTGC AGAGGGACAG TGAAGGAAGT AGGGTTTGCA 
CATTTATGGA AGGAGAGGGG GGTCCAAGGG CAGAGGGTGA AAGGTGTAGG ACAGGAGAGC 
CATGGAGAAT ATGAAGATAA AAAGAAGAAG GACAAGAGGA AGGAAAAAGA CAGATTTCCT 
GCAGATGAAG GGCGTCAGCG GCAAGACAGG CAGGCCGGCT GGTGGTACCA GCTCATGCAG 
TCCTCCCAGG TCTACATCGA CCAATCCACA GAGGGGACAA AGTTTGTTAA GACCGAGAAG 
AGGAAGAAGT CATCGGAGAG ACGACAGGGC CAAATCCCAC CCACAAGAGA GGGAGTGGTG 
GAGGGAGCGG AGGCCTCTCA GGAAGGTGAG GGCAGGAAGA GCAGCAGCAG TAGCTTGAGC 
GGGGACTCAT CTGGATCCAG GGGGCGGCCT TCGTGGATGG GCAGCCCCCC AGAGTCTGTC 
CTCAACCAGG AGAATCACAC CAACCCTCTG GAGGTCACGG TGACTGGGAG CCACGGCGGA 
GCCCAGGATG AGAGCCCCTC ACAGGGACAG AGTCTGCGCT GGAGCAGGCT CTTTGGCTCT 
AGTGTCGGTT CTCCCTCGAA AGCAGAAGGA GGTGAACAGA AGCCAAAAAC CCAAAAGACC 
AGACCTCCAT CTGGCTGGCT CGGCTTGGAT CGGTCTGTTC TCGACCTTGT AGCTTTGACC 
ATTGGAGCAG GCCCCGGAAA AAAGGCGGAG CTTCCTGCCA CACCAACTCG CACCCAAAGT 
ACAGCTCCAC CAGCGTCGAC GTACCCAGCT GAGGCCAAAC TACAGTCCAG CTGCGAAGTA 
CGAGCACTGT GCCACCACAT CGCCACCGAG CCCGGACAGC TGAGCTTCAA CAAGGGCGAC 
ATCCTGCGCG TTCTGAGCAA GGCTGACCCC GATTGGCTGC TCTGTTCCCT GGGCTCCACC 
CGGGGCTTGG TTCCAATTGT CTACGTCACC CTCAACAGCA TGGAGGACAG CCAGGATGCC 
CCTGGTTTCG GGCAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008323.1
CDS	391..5211
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_023152555.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152555.1

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCAGTGGGCC AATCAGCTAA GCTGCAGCAG 
AGCTACAGCG ACTTCCTGTC CGACTACTTT TCCCTGACAG AGAAGCCGCC TGAGGAGTTC 
TGCCTCTCCC CGGACGCCTC CACCTCCTCC TCTTTGTGCT CGTCCTCGCA GTCTCACATC 
TCTGTTGACC TGACGCAGAA GAGAGCTCTG GTGAAAGCCG TGAACACTGC AGTGGATCTG 
ATCGTGGCTC ACTTCGGCAC CAGCAGAGAC CCCGATGTTA AGGCAAAGCT CGGGAACAGC 
TGGGTGAGTC CCAACGTGGG TCACCTCATC CTGAAGTATC TGTGTCCGGC TCTACGTGAC 
GTGCTGCAGG ACGGTCTGAA GGCCTACGTG CTGGACCTGA TCATTGGACA GCGCCGCTGT 
CAGCCCTGGA GCCTCGTGGA GGCGTCCACA CAGCTGGGCC CGTCCACACG TGTTCTCCAC 
AGCCTGCTCT CAAAGGTGAG CCAGTACTCG GAGCTCACGA GCCACAGCAT GAGACTGAAT 
GCTTTCATCT TCGGCCTGCT CAATCTGAAA TCTTTGGAAT TCTGGTTCAA TCACCTCTAC 
ACGCATGAAG ATATTATTGC CGCATACTAC AGCCCTTGGG GTTTCCTTCC CCTGTCACAG 
GGGCCGTGTC AGCCCCTCTT TGAGGAGCTC CTCCTCCTGC TGCAGCCTCT CTCACTGCTC 
CCCTTTGACC TGGACCTGCT GTTTGAACCA CATCTCCTCC AAAAGGGACA AGAGCAGCTT 
CGTCGCAAAG AGCAGCTCTG CTCTGCAGGC CAGAGCGTCG ACCAGTCGAA GTGCTCCACC 
TTCCAGCTCA TGAGGGGGTG GAGCAACACT GTCAGTGAAA TGGTCAAAGA CTCAAGCGCA 
GAGGTGAAGA AAGAGAAGGC GGGGCTCAGG CGAGAGGGAA CATGGCCAAG GATGGAGGGA 
GTTGGATCGA GAAGAGAGGC AGCAGCGGGG AAATCGATGC TGAGGAAAGA GAGCGTTACA 
GCTCAGAGCA TGACAGCTGG GCCTGATTGC AGAGGGACAG TGAAGGAAGT AGGGTTTGCA 
CATTTATGGA AGGAGAGGGG GGTCCAAGGG CAGAGGGTGA AAGGTGTAGG ACAGGAGAGC 
CATGGAGAAT ATGAAGATAA AAAGAAGAAG GACAAGAGGA AGGAAAAAGA CAGATTTCCT 
GCAGATGAAG GGCGTCAGCG GCAAGACAGG CAGGCCGGCT GGTGGTACCA GCTCATGCAG 
TCCTCCCAGG TCTACATCGA CCAATCCACA GAGGGGACAA AGTTTGTTAA GACCGAGAAG 
AGGAAGAAGT CATCGGAGAG ACGACAGGGC CAAATCCCAC CCACAAGAGA GGGAGTGGTG 
GAGGGAGCGG AGGCCTCTCA GGAAGGTGAG GGCAGGAAGA GCAGCAGCAG TAGCTTGAGC 
GGGGACTCAT CTGGATCCAG GGGGCGGCCT TCGTGGATGG GCAGCCCCCC AGAGTCTGTC 
CTCAACCAGG AGAATCACAC CAACCCTCTG GAGGTCACGG TGACTGGGAG CCACGGCGGA 
GCCCAGGATG AGAGCCCCTC ACAGGGACAG AGTCTGCGCT GGAGCAGGCT CTTTGGCTCT 
AGTGTCGGTT CTCCCTCGAA AGCAGAAGGA GGTGAACAGA AGCCAAAAAC CCAAAAGACC 
AGACCTCCAT CTGGCTGGCT CGGCTTGGAT CGGTCTGTTC TCGACCTTGT AGCTTTGACC 
ATTGGAGCAG GCCCCGGAAA AAAGGCGGAG CTTCCTGCCA CACCAACTCG CACCCAAAGT 
ACAGCTCCAC CAGCGTCGAC GTACCCAGCT GAGGCCAAAC TACAGTCCAG CTGCGAAGTA 
CGAGCACTGT GCCACCACAT CGCCACCGAG CCCGGACAGC TGAGCTTCAA CAAGGGCGAC 
ATCCTGCGCG TTCTGAGCAA GGCTGACCCC GATTGGCTGC TCTGTTCCCT GGGCTCCACC 
CGGGGCTTGG TTCCAATTGT CTACGTCACC CTCAACAGCA TGGAGGACAG CCAGGATGCC 
CCTGGTTTCG GGCAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555657.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555714.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

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CloneID	OMa34673
Clone ID Related Accession (Same CDS sequence)	XM_004555657.3 , XM_004555657.2
Accession Version	XM_004555657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004555657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555714.1
CDS	391..5388
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555657.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555657.3

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555657.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555714.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa34673
Clone ID Related Accession (Same CDS sequence)	XM_004555657.3 , XM_004555657.2
Accession Version	XM_004555657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	iporin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004555657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555714.1
CDS	390..5387
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555657.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555657.2

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555658.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555715.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

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CloneID	OMa34674
Clone ID Related Accession (Same CDS sequence)	XM_004555658.2 , XM_004555658.3
Accession Version	XM_004555658.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	iporin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004555658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555715.1
CDS	390..5384
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555658.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555658.2

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555658.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555715.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

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CloneID	OMa34674
Clone ID Related Accession (Same CDS sequence)	XM_004555658.2 , XM_004555658.3
Accession Version	XM_004555658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004555658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555715.1
CDS	391..5385
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555658.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555658.3

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555659.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555716.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

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CloneID	OMa34675
Clone ID Related Accession (Same CDS sequence)	XM_004555659.2 , XM_004555659.3
Accession Version	XM_004555659.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	iporin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004555659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TACCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555716.1
CDS	390..5384
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555659.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555659.2

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TACCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555659.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555716.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

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CloneID	OMa34675
Clone ID Related Accession (Same CDS sequence)	XM_004555659.2 , XM_004555659.3
Accession Version	XM_004555659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004555659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TACCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555716.1
CDS	391..5385
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555659.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555659.3

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TACCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACAGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGCAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_012920345.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012775799.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

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CloneID	OMa34676
Clone ID Related Accession (Same CDS sequence)	XM_012920345.2 , XM_012920345.3
Accession Version	XM_012920345.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	iporin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012920345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTAC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACA GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGCAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012775799.1
CDS	390..5381
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_012920345.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012920345.2

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTAC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACA GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGCAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_012920345.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012775799.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMa34676
Clone ID Related Accession (Same CDS sequence)	XM_012920345.2 , XM_012920345.3
Accession Version	XM_012920345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_012920345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTAC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACA GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGCAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012775799.1
CDS	391..5382
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_012920345.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012920345.3

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTAC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACA GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGCAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555661.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555718.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

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CloneID	OMa34677
Clone ID Related Accession (Same CDS sequence)	XM_004555661.3 , XM_004555661.4
Accession Version	XM_004555661.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	iporin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004555661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555718.1
CDS	390..5207
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555661.3
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555661.3

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555661.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555718.1 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.

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CloneID	OMa34677
Clone ID Related Accession (Same CDS sequence)	XM_004555661.3 , XM_004555661.4
Accession Version	XM_004555661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004555661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555718.1
CDS	391..5208
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERRQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555661.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555661.4

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGAGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTACCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACAGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGCA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555657.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555714.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

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CloneID	OMa361235
Clone ID Related Accession (Same CDS sequence)	XM_004555657.4
Accession Version	XM_004555657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004555657.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACGGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGTAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555714.2
CDS	391..5385
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555657.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555657.4

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGCA GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACGGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGTAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555658.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555715.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

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CloneID	OMa361236
Clone ID Related Accession (Same CDS sequence)	XM_004555658.4
Accession Version	XM_004555658.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004555658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACGGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGTAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555715.2
CDS	387..5378
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555658.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555658.4

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCGCACCACC TCTCGCCTCA GGCTCTCAAA 
TGGAGGGAGT ACCGCCGTCG AAACCCTCTG GGAGTGGAGA GGGTGTCGGG GTGCCATGCT 
TCTGCTGGAT TGGGGCCGCG ACCTGCCAGA CGCAACGTTT TTGATTTCCC TTCGGTCCCC 
TCTGGCCACA AGCTGGGACG ACTCAACGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACGGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGTAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555659.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555716.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

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CloneID	OMa361237
Clone ID Related Accession (Same CDS sequence)	XM_004555659.4
Accession Version	XM_004555659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004555659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TGCCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACGGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGTAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555716.2
CDS	387..5378
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555659.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555659.4

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATGATAA TGACAATGAT GAGGATGAGG AGAAGAGGAA GAATCAGCAG 
GGAGCTAAAG CTGGTCCTGT GATTGAAGGC CAGGTGTATG TCAACATTTC TCCTCCTCTG 
TCTGGTCGGG GCGTTATTGG AGCGTCTGCA GGAAGCCGTC CACGTTCTCG CAGCTACGAT 
CGCAACCTGG ACAAGTCTCC GTCTCCACGG CTCGGCTCGC TGGAGCGGAT GCTGAGCTGC 
CCCGTCCGGC TCAGCGAAGG CGCTGCCGCG ACCCCACCTC CTCCTCCAAG GGTCACCTCC 
TTCGCAGAGA TAGCTAGAAG CAAACGAAGA AATGGAGGAG CGGGTGGATC GCCCTCGGTG 
AAGGCTGCTT CAGACGCCGT CTCCTCTCAC TCGCACTCCT CAGTGGACTT TTCTCCGATC 
CTGGAGCAGC GTGTCGAGGC TGACAGTCAG GCCCTGTCGC CTGTGCCCTT TACCAGATGT 
TACAGTCAAG GCAGCATAGA GCGACACCTG GAAGGGGGGA GAGAAACCCG GGCCAAGGCA 
GAAGGTGGTC TCTCCAGTTC TGCAGACAGC CGCTCAGCAG TGGTCCGCTA CAGCAAGGAC 
CAGCGGCCCA CGACCCTTCC CATCCAGCCC TTTACCTTCC ACCACCAGTT TGCAGCTAAA 
GCCCCCCAGC CCAAGCCCCT GGCGCCCCTG CTCACAGGCT ACATCTCTAG CATGCAGGCC 
CGCTCCAGCT TGGGCTCAGG CTCTGGAGGT CCAGTCGGGG AGGACGGCGA CGACGCAGCT 
GAGAAAGTCC ACAGCTTCCA GGGTTCTCTC ATCACAACGG CTCCTCCTCC TGGATCGGTT 
AGGCCCTCGC CTCTTGGGAG TTACTCCCCG GTCCGACTCC AGGGAGCACC CAGCTCCGGG 
ACCTGCTCCA CCTGCACCCC CAGCCCTAAG CCAACGCACA GCCTCTCCTG CCCCCTTTCA 
GCCGGCCTCG GCCCCCTGCA GACCCCGCCC ACAATAAAAC AGGCGGGGGC ATCAGCGCCG 
TTACCCCCTA CTCCTCCTCC TTCATCACTG GGGCTGAAGA GAGGGGCGGT GCCACCAATG 
CTGCCAGCAG TGCAGGGACA CTGTTTCCGC CGTGGAGGGC TGCCTGGCTT ACCAGCGCTT 
CACAGTGACA CTCTGAGCCC ACTGGGCTGC CTCGACTCTG CAAAGCACGA GGAGGGCAAC 
AAAGGGCACG CAGCCAGGAC ACATAATGCG CACCACCTCT CGCCTCAGGC TCTCAAATGG 
AGGGAGTACC GCCGTCGAAA CCCTCTGGGA GTGGAGAGGG TGTCGGGGTG CCATGCTTCT 
GCTGGATTGG GGCCGCGACC TGCCAGACGC AACGTTTTTG ATTTCCCTTC GGTCCCCTCT 
GGCCACAAGC TGGGACGACT CAACGCAGTG GGCCAATCAG CTAAGCTGCA GCAGAGCTAC 
AGCGACTTCC TGTCCGACTA CTTTTCCCTG ACGGAGAAGC CGCCTGAGGA GTTCTGCCTC 
TCCCCGGACG CCTCCACCTC CTCCTCTTTG TGCTCGTCCT CGCAGTCTCA CATCTCTGTT 
GACCTGACGC AGAAGAGAGC TCTGGTGAAA GCCGTGAACA CTGCAGTGGA TCTGATCGTG 
GCTCACTTCG GCACCAGCAG AGACCCCGAT GTTAAGGCAA AGCTCGGGAA CAGCTGGGTG 
AGTCCCAACG TGGGTCACCT CATCCTGAAG TATCTGTGTC CGGCTCTACG TGACGTGCTG 
CAGGACGGTC TGAAGGCCTA CGTGCTGGAC CTGATCATTG GACAGCGCCG CTGTCAGCCC 
TGGAGCCTCG TGGAGGCGTC CACACAGCTG GGCCCGTCCA CACGTGTTCT CCACAGCCTG 
CTCTCAAAGG TGAGCCAGTA CTCGGAGCTC ACGAGCCACA GCATGAGACT GAATGCTTTC 
ATCTTCGGCC TGCTCAATCT GAAATCTTTG GAATTCTGGT TCAATCACCT CTACACGCAT 
GAAGATATTA TTGCCGCATA CTACAGCCCT TGGGGTTTCC TTCCCCTGTC ACAGGGGCCG 
TGTCAGCCCC TCTTTGAGGA GCTCCTCCTC CTGCTGCAGC CTCTCTCACT GCTCCCCTTT 
GACCTGGACC TGCTGTTTGA ACCACATCTC CTCCAAAAGG GACAAGAGCA GCTTCGTCGC 
AAAGAGCAGC TCTGCTCTGC AGGCCAGAGC GTCGACCAGT CGAAGTGCTC CACCTTCCAG 
CTCATGAGGG GGTGGAGCAA CACTGTCAGT GAAATGGTCA AAGACTCAAG CGCAGAGGTG 
AAGAAAGAGA AGGCGGGGCT CAGGCGAGAG GGAACATGGC CAAGGATGGA GGGAGTTGGA 
TCGAGAAGAG AGGCAGCAGC GGGGAAATCG ATGCTGAGGA AAGAGAGCGT TACAGCTCAG 
AGCATGACAG CTGGGCCTGA TTGCAGAGGG ACAGTGAAGG AAGTAGGGTT TGCACATTTA 
TGGAAGGAGA GGGGGGTCCA AGGGCAGAGG GTGAAAGGTG TAGGACAGGA GAGCCATGGA 
GAATATGAAG ATAAAAAGAA GAAGGACAAG AGGAAGGAAA AAGACAGATT TCCTGCAGAT 
GAAGGGCGTC AGCGGCAAGA CAGGCAGGCC GGCTGGTGGT ACCAGCTCAT GCAGTCCTCC 
CAGGTCTACA TCGACCAATC CACAGAGGGG ACAAAGTTTG TTAAGACCGA GAAGAGGAAG 
AAGTCATCGG AGAGACAGGG CCAAATCCCA CCCACAAGAG AGGGAGTGGT GGAGGGAGCG 
GAGGCCTCTC AGGAAGGTGA GGGCAGGAAG AGCAGCAGCA GTAGCTTGAG CGGGGACTCA 
TCTGGATCCA GGGGGCGGCC TTCGTGGATG GGTAGCCCCC CAGAGTCTGT CCTCAACCAG 
GAGAATCACA CCAACCCTCT GGAGGTCACG GTGACTGGGA GCCACGGCGG AGCCCAGGAT 
GAGAGCCCCT CACAGGGACA GAGTCTGCGC TGGAGCAGGC TCTTTGGCTC TAGTGTCGGT 
TCTCCCTCGA AAGCAGAAGG AGGTGAACAG AAGCCAAAAA CCCAAAAGAC CAGACCTCCA 
TCTGGCTGGC TCGGCTTGGA TCGGTCTGTT CTCGACCTTG TAGCTTTGAC CATTGGAGCA 
GGCCCCGGAA AAAAGGCGGA GCTTCCTGCC ACACCAACTC GCACCCAAAG TACAGCTCCA 
CCAGCGTCGA CGTACCCAGC TGAGGCCAAA CTACAGTCCA GCTGCGAAGT ACGAGCACTG 
TGCCACCACA TCGCCACCGA GCCCGGACAG CTGAGCTTCA ACAAGGGCGA CATCCTGCGC 
GTTCTGAGCA AGGCTGACCC CGATTGGCTG CTCTGTTCCC TGGGCTCCAC CCGGGGCTTG 
GTTCCAATTG TCTACGTCAC CCTCAACAGC ATGGAGGACA GCCAGGATGC CCCTGGTTTC 
GGGCAATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_012920345.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012775799.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMa361238
Clone ID Related Accession (Same CDS sequence)	XM_012920345.4
Accession Version	XM_012920345.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012920345.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTGC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACG GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACAGGGCCA AATCCCACCC ACAAGAGAGG GAGTGGTGGA GGGAGCGGAG 
GCCTCTCAGG AAGGTGAGGG CAGGAAGAGC AGCAGCAGTA GCTTGAGCGG GGACTCATCT 
GGATCCAGGG GGCGGCCTTC GTGGATGGGT AGCCCCCCAG AGTCTGTCCT CAACCAGGAG 
AATCACACCA ACCCTCTGGA GGTCACGGTG ACTGGGAGCC ACGGCGGAGC CCAGGATGAG 
AGCCCCTCAC AGGGACAGAG TCTGCGCTGG AGCAGGCTCT TTGGCTCTAG TGTCGGTTCT 
CCCTCGAAAG CAGAAGGAGG TGAACAGAAG CCAAAAACCC AAAAGACCAG ACCTCCATCT 
GGCTGGCTCG GCTTGGATCG GTCTGTTCTC GACCTTGTAG CTTTGACCAT TGGAGCAGGC 
CCCGGAAAAA AGGCGGAGCT TCCTGCCACA CCAACTCGCA CCCAAAGTAC AGCTCCACCA 
GCGTCGACGT ACCCAGCTGA GGCCAAACTA CAGTCCAGCT GCGAAGTACG AGCACTGTGC 
CACCACATCG CCACCGAGCC CGGACAGCTG AGCTTCAACA AGGGCGACAT CCTGCGCGTT 
CTGAGCAAGG CTGACCCCGA TTGGCTGCTC TGTTCCCTGG GCTCCACCCG GGGCTTGGTT 
CCAATTGTCT ACGTCACCCT CAACAGCATG GAGGACAGCC AGGATGCCCC TGGTTTCGGG 
CAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012775799.2
CDS	387..5375
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAHHLSPQALKWREYRRRNPLGVERVSGCHASAGLGPRPARRNVFDFPSVPSGHKLGRLNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_012920345.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012920345.4

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
GATGATGATG ATGATAATGA CAATGATGAG GATGAGGAGA AGAGGAAGAA TCAGCAGGGA 
GCTAAAGCTG GTCCTGTGAT TGAAGGCCAG GTGTATGTCA ACATTTCTCC TCCTCTGTCT 
GGTCGGGGCG TTATTGGAGC GTCTGCAGGA AGCCGTCCAC GTTCTCGCAG CTACGATCGC 
AACCTGGACA AGTCTCCGTC TCCACGGCTC GGCTCGCTGG AGCGGATGCT GAGCTGCCCC 
GTCCGGCTCA GCGAAGGCGC TGCCGCGACC CCACCTCCTC CTCCAAGGGT CACCTCCTTC 
GCAGAGATAG CTAGAAGCAA ACGAAGAAAT GGAGGAGCGG GTGGATCGCC CTCGGTGAAG 
GCTGCTTCAG ACGCCGTCTC CTCTCACTCG CACTCCTCAG TGGACTTTTC TCCGATCCTG 
GAGCAGCGTG TCGAGGCTGA CAGTCAGGCC CTGTCGCCTG TGCCCTTTAC CAGATGTTAC 
AGTCAAGGCA GCATAGAGCG ACACCTGGAA GGGGGGAGAG AAACCCGGGC CAAGGCAGAA 
GGTGGTCTCT CCAGTTCTGC AGACAGCCGC TCAGCAGTGG TCCGCTACAG CAAGGACCAG 
CGGCCCACGA CCCTTCCCAT CCAGCCCTTT ACCTTCCACC ACCAGTTTGC AGCTAAAGCC 
CCCCAGCCCA AGCCCCTGGC GCCCCTGCTC ACAGGCTACA TCTCTAGCAT GCAGGCCCGC 
TCCAGCTTGG GCTCAGGCTC TGGAGGTCCA GTCGGGGAGG ACGGCGACGA CGCAGCTGAG 
AAAGTCCACA GCTTCCAGGG TTCTCTCATC ACAACGGCTC CTCCTCCTGG ATCGGTTAGG 
CCCTCGCCTC TTGGGAGTTA CTCCCCGGTC CGACTCCAGG GAGCACCCAG CTCCGGGACC 
TGCTCCACCT GCACCCCCAG CCCTAAGCCA ACGCACAGCC TCTCCTGCCC CCTTTCAGCC 
GGCCTCGGCC CCCTGCAGAC CCCGCCCACA ATAAAACAGG CGGGGGCATC AGCGCCGTTA 
CCCCCTACTC CTCCTCCTTC ATCACTGGGG CTGAAGAGAG GGGCGGTGCC ACCAATGCTG 
CCAGCAGTGC AGGGACACTG TTTCCGCCGT GGAGGGCTGC CTGGCTTACC AGCGCTTCAC 
AGTGACACTC TGAGCCCACT GGGCTGCCTC GACTCTGCAA AGCACGAGGA GGGCAACAAA 
GGGCACGCAG CCAGGACACA TAATGCGCAC CACCTCTCGC CTCAGGCTCT CAAATGGAGG 
GAGTACCGCC GTCGAAACCC TCTGGGAGTG GAGAGGGTGT CGGGGTGCCA TGCTTCTGCT 
GGATTGGGGC CGCGACCTGC CAGACGCAAC GTTTTTGATT TCCCTTCGGT CCCCTCTGGC 
CACAAGCTGG GACGACTCAA CGCAGTGGGC CAATCAGCTA AGCTGCAGCA GAGCTACAGC 
GACTTCCTGT CCGACTACTT TTCCCTGACG GAGAAGCCGC CTGAGGAGTT CTGCCTCTCC 
CCGGACGCCT CCACCTCCTC CTCTTTGTGC TCGTCCTCGC AGTCTCACAT CTCTGTTGAC 
CTGACGCAGA AGAGAGCTCT GGTGAAAGCC GTGAACACTG CAGTGGATCT GATCGTGGCT 
CACTTCGGCA CCAGCAGAGA CCCCGATGTT AAGGCAAAGC TCGGGAACAG CTGGGTGAGT 
CCCAACGTGG GTCACCTCAT CCTGAAGTAT CTGTGTCCGG CTCTACGTGA CGTGCTGCAG 
GACGGTCTGA AGGCCTACGT GCTGGACCTG ATCATTGGAC AGCGCCGCTG TCAGCCCTGG 
AGCCTCGTGG AGGCGTCCAC ACAGCTGGGC CCGTCCACAC GTGTTCTCCA CAGCCTGCTC 
TCAAAGGTGA GCCAGTACTC GGAGCTCACG AGCCACAGCA TGAGACTGAA TGCTTTCATC 
TTCGGCCTGC TCAATCTGAA ATCTTTGGAA TTCTGGTTCA ATCACCTCTA CACGCATGAA 
GATATTATTG CCGCATACTA CAGCCCTTGG GGTTTCCTTC CCCTGTCACA GGGGCCGTGT 
CAGCCCCTCT TTGAGGAGCT CCTCCTCCTG CTGCAGCCTC TCTCACTGCT CCCCTTTGAC 
CTGGACCTGC TGTTTGAACC ACATCTCCTC CAAAAGGGAC AAGAGCAGCT TCGTCGCAAA 
GAGCAGCTCT GCTCTGCAGG CCAGAGCGTC GACCAGTCGA AGTGCTCCAC CTTCCAGCTC 
ATGAGGGGGT GGAGCAACAC TGTCAGTGAA ATGGTCAAAG ACTCAAGCGC AGAGGTGAAG 
AAAGAGAAGG CGGGGCTCAG GCGAGAGGGA ACATGGCCAA GGATGGAGGG AGTTGGATCG 
AGAAGAGAGG CAGCAGCGGG GAAATCGATG CTGAGGAAAG AGAGCGTTAC AGCTCAGAGC 
ATGACAGCTG GGCCTGATTG CAGAGGGACA GTGAAGGAAG TAGGGTTTGC ACATTTATGG 
AAGGAGAGGG GGGTCCAAGG GCAGAGGGTG AAAGGTGTAG GACAGGAGAG CCATGGAGAA 
TATGAAGATA AAAAGAAGAA GGACAAGAGG AAGGAAAAAG ACAGATTTCC TGCAGATGAA 
GGGCGTCAGC GGCAAGACAG GCAGGCCGGC TGGTGGTACC AGCTCATGCA GTCCTCCCAG 
GTCTACATCG ACCAATCCAC AGAGGGGACA AAGTTTGTTA AGACCGAGAA GAGGAAGAAG 
TCATCGGAGA GACAGGGCCA AATCCCACCC ACAAGAGAGG GAGTGGTGGA GGGAGCGGAG 
GCCTCTCAGG AAGGTGAGGG CAGGAAGAGC AGCAGCAGTA GCTTGAGCGG GGACTCATCT 
GGATCCAGGG GGCGGCCTTC GTGGATGGGT AGCCCCCCAG AGTCTGTCCT CAACCAGGAG 
AATCACACCA ACCCTCTGGA GGTCACGGTG ACTGGGAGCC ACGGCGGAGC CCAGGATGAG 
AGCCCCTCAC AGGGACAGAG TCTGCGCTGG AGCAGGCTCT TTGGCTCTAG TGTCGGTTCT 
CCCTCGAAAG CAGAAGGAGG TGAACAGAAG CCAAAAACCC AAAAGACCAG ACCTCCATCT 
GGCTGGCTCG GCTTGGATCG GTCTGTTCTC GACCTTGTAG CTTTGACCAT TGGAGCAGGC 
CCCGGAAAAA AGGCGGAGCT TCCTGCCACA CCAACTCGCA CCCAAAGTAC AGCTCCACCA 
GCGTCGACGT ACCCAGCTGA GGCCAAACTA CAGTCCAGCT GCGAAGTACG AGCACTGTGC 
CACCACATCG CCACCGAGCC CGGACAGCTG AGCTTCAACA AGGGCGACAT CCTGCGCGTT 
CTGAGCAAGG CTGACCCCGA TTGGCTGCTC TGTTCCCTGG GCTCCACCCG GGGCTTGGTT 
CCAATTGTCT ACGTCACCCT CAACAGCATG GAGGACAGCC AGGATGCCCC TGGTTTCGGG 
CAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_023152555.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023008323.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

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CloneID	OMa361239
Clone ID Related Accession (Same CDS sequence)	XM_023152555.2
Accession Version	XM_023152555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023152555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCAGTGGGCC AATCAGCTAA GCTGCAGCAG 
AGCTACAGCG ACTTCCTGTC CGACTACTTT TCCCTGACGG AGAAGCCGCC TGAGGAGTTC 
TGCCTCTCCC CGGACGCCTC CACCTCCTCC TCTTTGTGCT CGTCCTCGCA GTCTCACATC 
TCTGTTGACC TGACGCAGAA GAGAGCTCTG GTGAAAGCCG TGAACACTGC AGTGGATCTG 
ATCGTGGCTC ACTTCGGCAC CAGCAGAGAC CCCGATGTTA AGGCAAAGCT CGGGAACAGC 
TGGGTGAGTC CCAACGTGGG TCACCTCATC CTGAAGTATC TGTGTCCGGC TCTACGTGAC 
GTGCTGCAGG ACGGTCTGAA GGCCTACGTG CTGGACCTGA TCATTGGACA GCGCCGCTGT 
CAGCCCTGGA GCCTCGTGGA GGCGTCCACA CAGCTGGGCC CGTCCACACG TGTTCTCCAC 
AGCCTGCTCT CAAAGGTGAG CCAGTACTCG GAGCTCACGA GCCACAGCAT GAGACTGAAT 
GCTTTCATCT TCGGCCTGCT CAATCTGAAA TCTTTGGAAT TCTGGTTCAA TCACCTCTAC 
ACGCATGAAG ATATTATTGC CGCATACTAC AGCCCTTGGG GTTTCCTTCC CCTGTCACAG 
GGGCCGTGTC AGCCCCTCTT TGAGGAGCTC CTCCTCCTGC TGCAGCCTCT CTCACTGCTC 
CCCTTTGACC TGGACCTGCT GTTTGAACCA CATCTCCTCC AAAAGGGACA AGAGCAGCTT 
CGTCGCAAAG AGCAGCTCTG CTCTGCAGGC CAGAGCGTCG ACCAGTCGAA GTGCTCCACC 
TTCCAGCTCA TGAGGGGGTG GAGCAACACT GTCAGTGAAA TGGTCAAAGA CTCAAGCGCA 
GAGGTGAAGA AAGAGAAGGC GGGGCTCAGG CGAGAGGGAA CATGGCCAAG GATGGAGGGA 
GTTGGATCGA GAAGAGAGGC AGCAGCGGGG AAATCGATGC TGAGGAAAGA GAGCGTTACA 
GCTCAGAGCA TGACAGCTGG GCCTGATTGC AGAGGGACAG TGAAGGAAGT AGGGTTTGCA 
CATTTATGGA AGGAGAGGGG GGTCCAAGGG CAGAGGGTGA AAGGTGTAGG ACAGGAGAGC 
CATGGAGAAT ATGAAGATAA AAAGAAGAAG GACAAGAGGA AGGAAAAAGA CAGATTTCCT 
GCAGATGAAG GGCGTCAGCG GCAAGACAGG CAGGCCGGCT GGTGGTACCA GCTCATGCAG 
TCCTCCCAGG TCTACATCGA CCAATCCACA GAGGGGACAA AGTTTGTTAA GACCGAGAAG 
AGGAAGAAGT CATCGGAGAG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGTA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023008323.2
CDS	387..5204
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNAVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_023152555.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023152555.2

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GCAGTGGGCC AATCAGCTAA GCTGCAGCAG 
AGCTACAGCG ACTTCCTGTC CGACTACTTT TCCCTGACGG AGAAGCCGCC TGAGGAGTTC 
TGCCTCTCCC CGGACGCCTC CACCTCCTCC TCTTTGTGCT CGTCCTCGCA GTCTCACATC 
TCTGTTGACC TGACGCAGAA GAGAGCTCTG GTGAAAGCCG TGAACACTGC AGTGGATCTG 
ATCGTGGCTC ACTTCGGCAC CAGCAGAGAC CCCGATGTTA AGGCAAAGCT CGGGAACAGC 
TGGGTGAGTC CCAACGTGGG TCACCTCATC CTGAAGTATC TGTGTCCGGC TCTACGTGAC 
GTGCTGCAGG ACGGTCTGAA GGCCTACGTG CTGGACCTGA TCATTGGACA GCGCCGCTGT 
CAGCCCTGGA GCCTCGTGGA GGCGTCCACA CAGCTGGGCC CGTCCACACG TGTTCTCCAC 
AGCCTGCTCT CAAAGGTGAG CCAGTACTCG GAGCTCACGA GCCACAGCAT GAGACTGAAT 
GCTTTCATCT TCGGCCTGCT CAATCTGAAA TCTTTGGAAT TCTGGTTCAA TCACCTCTAC 
ACGCATGAAG ATATTATTGC CGCATACTAC AGCCCTTGGG GTTTCCTTCC CCTGTCACAG 
GGGCCGTGTC AGCCCCTCTT TGAGGAGCTC CTCCTCCTGC TGCAGCCTCT CTCACTGCTC 
CCCTTTGACC TGGACCTGCT GTTTGAACCA CATCTCCTCC AAAAGGGACA AGAGCAGCTT 
CGTCGCAAAG AGCAGCTCTG CTCTGCAGGC CAGAGCGTCG ACCAGTCGAA GTGCTCCACC 
TTCCAGCTCA TGAGGGGGTG GAGCAACACT GTCAGTGAAA TGGTCAAAGA CTCAAGCGCA 
GAGGTGAAGA AAGAGAAGGC GGGGCTCAGG CGAGAGGGAA CATGGCCAAG GATGGAGGGA 
GTTGGATCGA GAAGAGAGGC AGCAGCGGGG AAATCGATGC TGAGGAAAGA GAGCGTTACA 
GCTCAGAGCA TGACAGCTGG GCCTGATTGC AGAGGGACAG TGAAGGAAGT AGGGTTTGCA 
CATTTATGGA AGGAGAGGGG GGTCCAAGGG CAGAGGGTGA AAGGTGTAGG ACAGGAGAGC 
CATGGAGAAT ATGAAGATAA AAAGAAGAAG GACAAGAGGA AGGAAAAAGA CAGATTTCCT 
GCAGATGAAG GGCGTCAGCG GCAAGACAGG CAGGCCGGCT GGTGGTACCA GCTCATGCAG 
TCCTCCCAGG TCTACATCGA CCAATCCACA GAGGGGACAA AGTTTGTTAA GACCGAGAAG 
AGGAAGAAGT CATCGGAGAG ACAGGGCCAA ATCCCACCCA CAAGAGAGGG AGTGGTGGAG 
GGAGCGGAGG CCTCTCAGGA AGGTGAGGGC AGGAAGAGCA GCAGCAGTAG CTTGAGCGGG 
GACTCATCTG GATCCAGGGG GCGGCCTTCG TGGATGGGTA GCCCCCCAGA GTCTGTCCTC 
AACCAGGAGA ATCACACCAA CCCTCTGGAG GTCACGGTGA CTGGGAGCCA CGGCGGAGCC 
CAGGATGAGA GCCCCTCACA GGGACAGAGT CTGCGCTGGA GCAGGCTCTT TGGCTCTAGT 
GTCGGTTCTC CCTCGAAAGC AGAAGGAGGT GAACAGAAGC CAAAAACCCA AAAGACCAGA 
CCTCCATCTG GCTGGCTCGG CTTGGATCGG TCTGTTCTCG ACCTTGTAGC TTTGACCATT 
GGAGCAGGCC CCGGAAAAAA GGCGGAGCTT CCTGCCACAC CAACTCGCAC CCAAAGTACA 
GCTCCACCAG CGTCGACGTA CCCAGCTGAG GCCAAACTAC AGTCCAGCTG CGAAGTACGA 
GCACTGTGCC ACCACATCGC CACCGAGCCC GGACAGCTGA GCTTCAACAA GGGCGACATC 
CTGCGCGTTC TGAGCAAGGC TGACCCCGAT TGGCTGCTCT GTTCCCTGGG CTCCACCCGG 
GGCTTGGTTC CAATTGTCTA CGTCACCCTC AACAGCATGG AGGACAGCCA GGATGCCCCT 
GGTTTCGGGC AATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rusc2 ( XM_004555661.5 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004555718.2 Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.

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CloneID	OMa361240
Clone ID Related Accession (Same CDS sequence)	XM_004555661.5
Accession Version	XM_004555661.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	iporin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036791.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004555661.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACGGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGTAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004555718.2
CDS	387..5201
Translation	MDSPPKLSGETLIVHHIPLVHCQVSSRRQGGCGSSMKRTNPFSPPENLGLSRTTSLPERDVLQREALLYSSLIQTSSGSWSSHNGGRERKGGGRGGSTASDDSSFASSNSEDQLITAHTLPRAKPRNRNPFRQNPFLLNTEEDEDDEGEEDDGDNLSGYLEDSSFHLHSDAHSALSDGLTSFHLHDLGFAPQPFLLHSSVGGSSGESLRGGASDLSNHLEDLEILELASQRRHGSSGSNMSMDCGDQDWGDDDEEDEDHPMRCGRSSKTGSYSSSSSTQHCSCCTLSQNYPRHFPEAFSEPFLECQQGYGSDSSCNSSDGVLVNFSAIYNKMNNSVPEKPPVSGPTNLNSSTDHSCTSSASDLPGNRDSTGGAFYLDLHTSPTEPPLSQQLGNTFPLIREPRLSSSSTCSCSVEHQGALDLDANCNSYHPPHAGSSGDLASCLQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSVHSCSDEHSKGSPTPTQPSEYFLFRQQAYEEGVEEEDEEGETSRQDDDDDDNDNDEDEEKRKNQQGAKAGPVIEGQVYVNISPPLSGRGVIGASAGSRPRSRSYDRNLDKSPSPRLGSLERMLSCPVRLSEGAAATPPPPPRVTSFAEIARSKRRNGGAGGSPSVKAASDAVSSHSHSSVDFSPILEQRVEADSQALSPVPFTRCYSQGSIERHLEGGRETRAKAEGGLSSSADSRSAVVRYSKDQRPTTLPIQPFTFHHQFAAKAPQPKPLAPLLTGYISSMQARSSLGSGSGGPVGEDGDDAAEKVHSFQGSLITTAPPPGSVRPSPLGSYSPVRLQGAPSSGTCSTCTPSPKPTHSLSCPLSAGLGPLQTPPTIKQAGASAPLPPTPPPSSLGLKRGAVPPMLPAVQGHCFRRGGLPGLPALHSDTLSPLGCLDSAKHEEGNKGHAARTHNVGQSAKLQQSYSDFLSDYFSLTEKPPEEFCLSPDASTSSSLCSSSQSHISVDLTQKRALVKAVNTAVDLIVAHFGTSRDPDVKAKLGNSWVSPNVGHLILKYLCPALRDVLQDGLKAYVLDLIIGQRRCQPWSLVEASTQLGPSTRVLHSLLSKVSQYSELTSHSMRLNAFIFGLLNLKSLEFWFNHLYTHEDIIAAYYSPWGFLPLSQGPCQPLFEELLLLLQPLSLLPFDLDLLFEPHLLQKGQEQLRRKEQLCSAGQSVDQSKCSTFQLMRGWSNTVSEMVKDSSAEVKKEKAGLRREGTWPRMEGVGSRREAAAGKSMLRKESVTAQSMTAGPDCRGTVKEVGFAHLWKERGVQGQRVKGVGQESHGEYEDKKKKDKRKEKDRFPADEGRQRQDRQAGWWYQLMQSSQVYIDQSTEGTKFVKTEKRKKSSERQGQIPPTREGVVEGAEASQEGEGRKSSSSSLSGDSSGSRGRPSWMGSPPESVLNQENHTNPLEVTVTGSHGGAQDESPSQGQSLRWSRLFGSSVGSPSKAEGGEQKPKTQKTRPPSGWLGLDRSVLDLVALTIGAGPGKKAELPATPTRTQSTAPPASTYPAEAKLQSSCEVRALCHHIATEPGQLSFNKGDILRVLSKADPDWLLCSLGSTRGLVPIVYVTLNSMEDSQDAPGFGQC

Target ORF information:

RefSeq Version	XM_004555661.5
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra RUN and SH3 domain containing 2 (rusc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004555661.5

ORF Insert Sequence:

ATGGACAGCC CTCCTAAACT GTCTGGTGAG ACGCTGATTG TGCACCACAT TCCCCTGGTG 
CACTGTCAGG TGTCGAGTAG GCGGCAGGGT GGCTGCGGGA GCTCAATGAA GAGGACCAAC 
CCTTTTAGCC CACCAGAGAA CCTGGGCTTG AGTCGCACCA CTTCCCTTCC GGAGAGAGAC 
GTCCTCCAGA GAGAAGCTCT CCTCTACAGC AGCCTGATCC AGACCTCTAG TGGCTCCTGG 
TCCTCCCACA ATGGAGGAAG AGAAAGAAAG GGTGGCGGGA GAGGCGGCAG CACAGCCAGC 
GATGATTCAT CATTTGCCTC GAGCAATTCG GAGGACCAGC TGATTACAGC GCACACATTA 
CCCAGAGCCA AACCGAGGAA CAGGAATCCA TTCCGTCAAA ATCCTTTTCT GCTGAATACC 
GAAGAGGACG AGGATGACGA AGGGGAAGAA GATGACGGTG ACAACCTCAG TGGTTACCTT 
GAAGACTCTT CCTTTCACCT TCACAGTGAC GCTCACTCTG CTCTCAGTGA CGGTTTGACT 
TCCTTCCATT TGCACGACCT TGGTTTTGCT CCCCAGCCCT TCCTCCTTCA CAGCTCAGTG 
GGAGGAAGTA GTGGGGAGTC CCTGAGAGGT GGCGCCTCGG ATCTTTCAAA CCACCTAGAA 
GACCTCGAAA TTTTGGAACT TGCCAGTCAA CGGCGCCATG GAAGCAGTGG CTCCAACATG 
TCGATGGACT GCGGAGATCA AGATTGGGGT GATGACGACG AAGAAGACGA AGATCATCCG 
ATGAGGTGTG GGAGAAGCAG CAAGACAGGC TCCTACTCTT CCAGCTCTTC CACCCAGCAC 
TGCTCCTGCT GTACTCTCTC CCAGAATTAC CCTCGGCACT TCCCCGAGGC GTTCTCCGAG 
CCGTTCTTAG AGTGCCAGCA AGGCTACGGC AGCGACTCCT CCTGCAACAG CTCCGATGGT 
GTGCTCGTCA ACTTCAGTGC CATCTACAAC AAGATGAACA ACAGCGTCCC AGAGAAGCCA 
CCAGTGTCCG GTCCCACCAA CCTCAACAGC TCCACCGACC ACTCCTGCAC CTCATCTGCT 
TCCGATCTAC CAGGAAACCG AGACTCAACT GGAGGGGCTT TCTATTTGGA CCTTCACACT 
TCTCCAACCG AACCTCCCCT ATCTCAACAA CTTGGAAACA CATTCCCTCT CATCCGTGAA 
CCACGCCTGT CCTCTTCCTC CACCTGTTCC TGCTCAGTGG AGCACCAGGG GGCTCTTGAC 
CTCGATGCCA ACTGCAACTC CTACCACCCT CCTCACGCAG GGTCCTCTGG CGACCTCGCT 
TCTTGTCTCC AGAGTCAGGC ACGACTGGTC GTCGCCACCC AGAACTACTA CAAACTGGTC 
ACGTGCGACC TTTCGTCGCA GTCGTCCCCG AGTCCCGCGG GCTCCTCGGT TCACAGTTGC 
TCAGACGAAC ACAGCAAAGG CAGCCCCACC CCGACTCAGC CGAGTGAGTA CTTCCTCTTC 
AGGCAGCAAG CTTACGAGGA AGGAGTGGAG GAAGAGGATG AAGAGGGAGA GACATCTAGG 
CAGGATGATG ATGATGATGA TAATGACAAT GATGAGGATG AGGAGAAGAG GAAGAATCAG 
CAGGGAGCTA AAGCTGGTCC TGTGATTGAA GGCCAGGTGT ATGTCAACAT TTCTCCTCCT 
CTGTCTGGTC GGGGCGTTAT TGGAGCGTCT GCAGGAAGCC GTCCACGTTC TCGCAGCTAC 
GATCGCAACC TGGACAAGTC TCCGTCTCCA CGGCTCGGCT CGCTGGAGCG GATGCTGAGC 
TGCCCCGTCC GGCTCAGCGA AGGCGCTGCC GCGACCCCAC CTCCTCCTCC AAGGGTCACC 
TCCTTCGCAG AGATAGCTAG AAGCAAACGA AGAAATGGAG GAGCGGGTGG ATCGCCCTCG 
GTGAAGGCTG CTTCAGACGC CGTCTCCTCT CACTCGCACT CCTCAGTGGA CTTTTCTCCG 
ATCCTGGAGC AGCGTGTCGA GGCTGACAGT CAGGCCCTGT CGCCTGTGCC CTTTACCAGA 
TGTTACAGTC AAGGCAGCAT AGAGCGACAC CTGGAAGGGG GGAGAGAAAC CCGGGCCAAG 
GCAGAAGGTG GTCTCTCCAG TTCTGCAGAC AGCCGCTCAG CAGTGGTCCG CTACAGCAAG 
GACCAGCGGC CCACGACCCT TCCCATCCAG CCCTTTACCT TCCACCACCA GTTTGCAGCT 
AAAGCCCCCC AGCCCAAGCC CCTGGCGCCC CTGCTCACAG GCTACATCTC TAGCATGCAG 
GCCCGCTCCA GCTTGGGCTC AGGCTCTGGA GGTCCAGTCG GGGAGGACGG CGACGACGCA 
GCTGAGAAAG TCCACAGCTT CCAGGGTTCT CTCATCACAA CGGCTCCTCC TCCTGGATCG 
GTTAGGCCCT CGCCTCTTGG GAGTTACTCC CCGGTCCGAC TCCAGGGAGC ACCCAGCTCC 
GGGACCTGCT CCACCTGCAC CCCCAGCCCT AAGCCAACGC ACAGCCTCTC CTGCCCCCTT 
TCAGCCGGCC TCGGCCCCCT GCAGACCCCG CCCACAATAA AACAGGCGGG GGCATCAGCG 
CCGTTACCCC CTACTCCTCC TCCTTCATCA CTGGGGCTGA AGAGAGGGGC GGTGCCACCA 
ATGCTGCCAG CAGTGCAGGG ACACTGTTTC CGCCGTGGAG GGCTGCCTGG CTTACCAGCG 
CTTCACAGTG ACACTCTGAG CCCACTGGGC TGCCTCGACT CTGCAAAGCA CGAGGAGGGC 
AACAAAGGGC ACGCAGCCAG GACACATAAT GTGGGCCAAT CAGCTAAGCT GCAGCAGAGC 
TACAGCGACT TCCTGTCCGA CTACTTTTCC CTGACGGAGA AGCCGCCTGA GGAGTTCTGC 
CTCTCCCCGG ACGCCTCCAC CTCCTCCTCT TTGTGCTCGT CCTCGCAGTC TCACATCTCT 
GTTGACCTGA CGCAGAAGAG AGCTCTGGTG AAAGCCGTGA ACACTGCAGT GGATCTGATC 
GTGGCTCACT TCGGCACCAG CAGAGACCCC GATGTTAAGG CAAAGCTCGG GAACAGCTGG 
GTGAGTCCCA ACGTGGGTCA CCTCATCCTG AAGTATCTGT GTCCGGCTCT ACGTGACGTG 
CTGCAGGACG GTCTGAAGGC CTACGTGCTG GACCTGATCA TTGGACAGCG CCGCTGTCAG 
CCCTGGAGCC TCGTGGAGGC GTCCACACAG CTGGGCCCGT CCACACGTGT TCTCCACAGC 
CTGCTCTCAA AGGTGAGCCA GTACTCGGAG CTCACGAGCC ACAGCATGAG ACTGAATGCT 
TTCATCTTCG GCCTGCTCAA TCTGAAATCT TTGGAATTCT GGTTCAATCA CCTCTACACG 
CATGAAGATA TTATTGCCGC ATACTACAGC CCTTGGGGTT TCCTTCCCCT GTCACAGGGG 
CCGTGTCAGC CCCTCTTTGA GGAGCTCCTC CTCCTGCTGC AGCCTCTCTC ACTGCTCCCC 
TTTGACCTGG ACCTGCTGTT TGAACCACAT CTCCTCCAAA AGGGACAAGA GCAGCTTCGT 
CGCAAAGAGC AGCTCTGCTC TGCAGGCCAG AGCGTCGACC AGTCGAAGTG CTCCACCTTC 
CAGCTCATGA GGGGGTGGAG CAACACTGTC AGTGAAATGG TCAAAGACTC AAGCGCAGAG 
GTGAAGAAAG AGAAGGCGGG GCTCAGGCGA GAGGGAACAT GGCCAAGGAT GGAGGGAGTT 
GGATCGAGAA GAGAGGCAGC AGCGGGGAAA TCGATGCTGA GGAAAGAGAG CGTTACAGCT 
CAGAGCATGA CAGCTGGGCC TGATTGCAGA GGGACAGTGA AGGAAGTAGG GTTTGCACAT 
TTATGGAAGG AGAGGGGGGT CCAAGGGCAG AGGGTGAAAG GTGTAGGACA GGAGAGCCAT 
GGAGAATATG AAGATAAAAA GAAGAAGGAC AAGAGGAAGG AAAAAGACAG ATTTCCTGCA 
GATGAAGGGC GTCAGCGGCA AGACAGGCAG GCCGGCTGGT GGTACCAGCT CATGCAGTCC 
TCCCAGGTCT ACATCGACCA ATCCACAGAG GGGACAAAGT TTGTTAAGAC CGAGAAGAGG 
AAGAAGTCAT CGGAGAGACA GGGCCAAATC CCACCCACAA GAGAGGGAGT GGTGGAGGGA 
GCGGAGGCCT CTCAGGAAGG TGAGGGCAGG AAGAGCAGCA GCAGTAGCTT GAGCGGGGAC 
TCATCTGGAT CCAGGGGGCG GCCTTCGTGG ATGGGTAGCC CCCCAGAGTC TGTCCTCAAC 
CAGGAGAATC ACACCAACCC TCTGGAGGTC ACGGTGACTG GGAGCCACGG CGGAGCCCAG 
GATGAGAGCC CCTCACAGGG ACAGAGTCTG CGCTGGAGCA GGCTCTTTGG CTCTAGTGTC 
GGTTCTCCCT CGAAAGCAGA AGGAGGTGAA CAGAAGCCAA AAACCCAAAA GACCAGACCT 
CCATCTGGCT GGCTCGGCTT GGATCGGTCT GTTCTCGACC TTGTAGCTTT GACCATTGGA 
GCAGGCCCCG GAAAAAAGGC GGAGCTTCCT GCCACACCAA CTCGCACCCA AAGTACAGCT 
CCACCAGCGT CGACGTACCC AGCTGAGGCC AAACTACAGT CCAGCTGCGA AGTACGAGCA 
CTGTGCCACC ACATCGCCAC CGAGCCCGGA CAGCTGAGCT TCAACAAGGG CGACATCCTG 
CGCGTTCTGA GCAAGGCTGA CCCCGATTGG CTGCTCTGTT CCCTGGGCTC CACCCGGGGC 
TTGGTTCCAA TTGTCTACGT CACCCTCAAC AGCATGGAGG ACAGCCAGGA TGCCCCTGGT 
TTCGGGCAAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rusc2
Entrez Gene ID	101466259
Full Name	RUN and SH3 domain containing 2
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rusc2 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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