HECW2 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	100666713
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Loxodonta africana(African savanna elephant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003406158.2	XP_003406206.1	E3 ubiquitin-protein ligase HECW2
XM_023542646.1	XP_023398414.1	E3 ubiquitin-protein ligase HECW2

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLo95651	XM_023542646.1 Latest version!	Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OLo33735	XM_003406158.2 Latest version!	Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_023542646.1 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_023398414.1 Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OLo95651
Clone ID Related Accession (Same CDS sequence)	XM_023542646.1
Accession Version	XM_023542646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515945600000
Organism	Loxodonta africana(African savanna elephant)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573423.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Loxodonta africana Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TACTACCATG GCATCAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCTGCTGTGA TGATGGGGGC GGAAGGCATG 
GAAGGAGGTG TGTCAGGAAG TTCGCACTCT CGGAAACTCG TCAGCTTTAC ACTGTCAGAT 
CTCAGGGCGG TTGGTCTAAA GAAAGGCATG TTCTTCAATC CTGATCCGTA TCTTAAGATG 
TCCATCCAGC CAGGTAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGA 
AGGTCTACTA TCATCAGTAA CACCACGAAT CCAATTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTTC TAACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCTATCATCA AACGTTTTCT GGGAAAACTA ACTATCCCGG TCCAGAGGCT GCTGGAGCGG 
CAGGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAGAGTGGAG GTTACATCTT CTGTTCATGA AGATGCTTCC 
CCAGAAGCTG TGGGCACAAT ACTTGGCGTC AGTACTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGATG AGGACATGCC AGGGGGCCAC CATGACAGCC AAGTGTGTTC CAATGGACCA 
ACATCTGAGG AAAGTGCTGC TGATGGGACC CCCAAACATT CTCTCAGGAC TAGCTCCACT 
TTGGAAATAG ACACAGAGGA ATTAATCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGG 
CGTCAGGATT CACTCAATGA TTATTTAGAT GCTATTGAAC ATAATGGCCA TTCCAGGCCA 
GGGGCAGCTA CTTGCTCGGA GAGGTCCATG GGAGCCTCTC CAAAACTAAG GAGTAGTTTT 
CCCACGGACA CAAGACTCAA CGCGATGCTT CATATCGACT CAGATGAAGA AGATCACGAG 
TTTCAGCAAG ACCTGGGTTA TCCGTCTTCC TTGGAGGAGG AAGGCGGATT AATAATGTTT 
AGCAGGGCAT CCAGAGCTGA TGACGATGGG AGCCTGGCAT CTCAGACGAA GCCAGAAGAC 
AACCCAGTTG AGAATGAAGA AGCCTTCGCA AATGAAGCTA CTTCCTTCGA GGATAAGCCG 
GAAAATCATG CAGAGCTTGC AGATAGCACC TTACCCTCAG GCCCATCCCC AGATGAAAAC 
GAAAATGGCC CAGAGTCTCA GCAAAGTGCT GACCAGGGCA GCGGTGAGTT GTGTGGTTCT 
CAAGAGATGG ATCAGCCCAC AAGTGGGGCA GATACGGGAA CTTCCGATAC ATCAGGAGGA 
AGCCGAAGAG CTGTCAGTGA GACTGAATCA CTTGATCAGG GGTCTGAGCC TTCCCAGGTG 
TCCTCTGAAA CGGAACCCAG TGACCCTGCC AGGACAGAGA GTGTGAGTGA GGCCAGCACC 
AGGCCCGAGG GAGAGAGTGA CCTGGAAGGC GCCGATAGCT CTTGCAATGA GAGTGTTACC 
ACGCAGCTGT CCTCGGTGGA GACACGCTGC TCGTCTCTTG AGAGTGCACG GTTTCCAGAA 
ACCCCTGCTT TTTCTTCTCA GGAGGAGGAA GATGGAGTCT GTGCAGCAGA GCCCACCAGC 
AGTGGCCCTG CCGAAGGGCC ACAGGAGTCC GTTTCCACTC CTGGTTCTTT ACCTGTGGTG 
CAAGTGCCCA GTGGGGAGGA GGAAGGGCCA GAATCAGCTA CTTTGCCTGA CCAAGAGGAG 
ATGGGAGCGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CCACTGCTGA GAACCAGCCC 
CAAGAAGAGG GTGATGCTGG GGATGCCCAA GGCACTTGTG AAGGGGCCAC TGCCCAGGAC 
GAGGGTGCGA CGGGAGGTTC CCAAGCCAAC GGCCACCAGC CATTGAGATC ACTACCTTCA 
GTACGCCAGG ACGTGAGCCG ATACCAGAGG GTGGACGAGG CGCTCCCACC AAACTGGGAG 
GCACGCATTG ACAGCCACGG GAGGATCTTT TATGTGGATC ATGTAAATAG AACCACGACA 
TGGCAGCGGC CGACAGCTCC CCCAGCCCCA CAGATGCTTC AGAGATCCAA CTCCATCCAG 
CAAATGGAGC AGTTGAACCG GCGATATCAG AGTATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TCTTGATGGG GCAGGGGAGG AAGCTGACTT TCACCAAGCC 
AGTGCAGATT TCCGACGGGA GAACGTTCTT CCCCACTCTA CCTCCAGATC CAGGCTCACG 
TTGCTGTTAC AGTCTCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCCAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCATCACTTT GAACGTTACC AACATAACCG GGACCTTGTG 
GGATTCCTCA ACATGTTTGC AAACAAACAG CTGGAGCTGC CGAGGGGCTG GGAAATGAAA 
CATGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAATT CCCGTACCAC CACTTTCATC 
GATCCTCGGC TCCCGCTTCA GAGCAGTAGA CCCACAAGTG CACTGGTTCA TCGGCAGCAC 
TTGACCAGGC AGCGCAGTCA CAGTGCGGGT GAGGTAGGAG AAGATTCCCG ACATGCAGGA 
CCACCTGTTC TTCCCAGGCC ATCCAGTACA TTCAACACAG TCAGTAGGCC ACAGTACCAG 
GACATGGTTC CAGTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGTCA ACCCAATATC 
TTTGAAATTC TACAGGAGCG TCAACCTGAT CTTACCAGGA ATCACTCACT CAGGGAGAAG 
ATTCAGTTTA TCCGAACTGA AGGAACCCCA GGATTGGTGC GTCTTTCAAG TGATGCAGAC 
CTTGTTATGT TGCTGAGTTT ATTTGAAGAA GAGATAATGT CATATGTGCC TCCACATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCA CGTGGCTCCC CTGTGTCCTC TCCTCAGAAC 
TCGCCAGGTG TGTCCCAGAT CTCTCACCTG AACTCCAGTT CTCTATTTTC AAGTGTCCAT 
AGAGCAGTTG CCCACCTGAC CAGCTATCAC CTCCAACTCA GTACTCAGCG TGCCAATGCC 
CGGGCTCCAG CCCCTTACAA GCGAGATTTT GAAGCCAAAC TAAGGAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGAAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTACTGG AAGATGCTTT TAATCAAATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTAT ATGTCACATT TGTTGGTGAG GAAGGGCTGG ATTACAGTGG ACCATCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCGT ATTATGGCCT ATTTGAATAT 
TCAGCCAATG ATACATACAC AGTACAAATA AGCCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TTCGGTTCAG TGGTCGGATC CTTGGTCTTG CACTCATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCGTTTTAT AAGGCTCTTC TCAGAATCCT GTGTGACCTG 
AGTGATCTAG AATATCTTGA TGAAGAGTTC CACCAGAGCC TGCAGTGGAT GAAAGATAAC 
GATATCCATG ACATCCTGGA CCTGACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACGGAACGAG AATTGAAGCC AGGGGGTGCC AATATCCCAG TCACAGAGAA GAACAAGAAG 
GAATACATCG AGAGGATGGT GAAGTGGAGA ATTGAGAGGG GTGTTGTACA ACAAACAGAG 
AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAGCTGG AACTGGTCAT TGCGGGCACA GCTGAAATAG ACTTAAGTGA TTGGAGAAAC 
AACACAGAAT ATAGAGGAGG ATACCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA CGAACAGCGA CTAAGGTTGT TACAGTTTGT CACAGGCACG 
TCCAGCATTC CCTACGAAGG ATTTGCATCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACTGCT CTTCCCAGGG CCCATACTTG TTTTAACCGT 
CTGGATCTGC CCCCATACCC ATCCTTCTCC ATGCTTTATG AAAAACTCTT GACAGCAGTT 
GAAGAGACGA GTACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023398414.1
CDS	638..5047
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGVSGSSHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVSTVNGDLGSPSDDEDMPGGHHDSQVCSNGPTSEESAADGTPKHSLRTSSTLEIDTEELISTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDDGSLASQTKPEDNPVENEEAFANEATSFEDKPENHAELADSTLPSGPSPDENENGPESQQSADQGSGELCGSQEMDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGVCAAEPTSSGPAEGPQESVSTPGSLPVVQVPSGEEEGPESATLPDQEEMGAVWQRRGSLEGATAENQPQEEGDAGDAQGTCEGATAQDEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQMLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSALDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGVSQISHLNSSSLFSSVHRAVAHLTSYHLQLSTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_023542646.1
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023542646.1

ORF Insert Sequence:

ATGGAACCGG AGATAAAAAT CTGTTTTAAA TACTACCATG GCATCAGTGG AGCCCTGCGA 
GCCACAACCC CCTGCATCAC CGTGAAGAAC CCTGCTGTGA TGATGGGGGC GGAAGGCATG 
GAAGGAGGTG TGTCAGGAAG TTCGCACTCT CGGAAACTCG TCAGCTTTAC ACTGTCAGAT 
CTCAGGGCGG TTGGTCTAAA GAAAGGCATG TTCTTCAATC CTGATCCGTA TCTTAAGATG 
TCCATCCAGC CAGGTAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGA 
AGGTCTACTA TCATCAGTAA CACCACGAAT CCAATTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTTC TAACTGATGT CTTAGAAATT GAAATTAAAG ACAAATTTGC CAAGAGCCGT 
CCTATCATCA AACGTTTTCT GGGAAAACTA ACTATCCCGG TCCAGAGGCT GCTGGAGCGG 
CAGGCCATCG GTGATCAAAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAC 
GTGAGTGGGT ACCTCCAGTT TAGAGTGGAG GTTACATCTT CTGTTCATGA AGATGCTTCC 
CCAGAAGCTG TGGGCACAAT ACTTGGCGTC AGTACTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGATG AGGACATGCC AGGGGGCCAC CATGACAGCC AAGTGTGTTC CAATGGACCA 
ACATCTGAGG AAAGTGCTGC TGATGGGACC CCCAAACATT CTCTCAGGAC TAGCTCCACT 
TTGGAAATAG ACACAGAGGA ATTAATCTCT ACTTCTTCAA GGACCTCACC TCCCAGAGGG 
CGTCAGGATT CACTCAATGA TTATTTAGAT GCTATTGAAC ATAATGGCCA TTCCAGGCCA 
GGGGCAGCTA CTTGCTCGGA GAGGTCCATG GGAGCCTCTC CAAAACTAAG GAGTAGTTTT 
CCCACGGACA CAAGACTCAA CGCGATGCTT CATATCGACT CAGATGAAGA AGATCACGAG 
TTTCAGCAAG ACCTGGGTTA TCCGTCTTCC TTGGAGGAGG AAGGCGGATT AATAATGTTT 
AGCAGGGCAT CCAGAGCTGA TGACGATGGG AGCCTGGCAT CTCAGACGAA GCCAGAAGAC 
AACCCAGTTG AGAATGAAGA AGCCTTCGCA AATGAAGCTA CTTCCTTCGA GGATAAGCCG 
GAAAATCATG CAGAGCTTGC AGATAGCACC TTACCCTCAG GCCCATCCCC AGATGAAAAC 
GAAAATGGCC CAGAGTCTCA GCAAAGTGCT GACCAGGGCA GCGGTGAGTT GTGTGGTTCT 
CAAGAGATGG ATCAGCCCAC AAGTGGGGCA GATACGGGAA CTTCCGATAC ATCAGGAGGA 
AGCCGAAGAG CTGTCAGTGA GACTGAATCA CTTGATCAGG GGTCTGAGCC TTCCCAGGTG 
TCCTCTGAAA CGGAACCCAG TGACCCTGCC AGGACAGAGA GTGTGAGTGA GGCCAGCACC 
AGGCCCGAGG GAGAGAGTGA CCTGGAAGGC GCCGATAGCT CTTGCAATGA GAGTGTTACC 
ACGCAGCTGT CCTCGGTGGA GACACGCTGC TCGTCTCTTG AGAGTGCACG GTTTCCAGAA 
ACCCCTGCTT TTTCTTCTCA GGAGGAGGAA GATGGAGTCT GTGCAGCAGA GCCCACCAGC 
AGTGGCCCTG CCGAAGGGCC ACAGGAGTCC GTTTCCACTC CTGGTTCTTT ACCTGTGGTG 
CAAGTGCCCA GTGGGGAGGA GGAAGGGCCA GAATCAGCTA CTTTGCCTGA CCAAGAGGAG 
ATGGGAGCGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CCACTGCTGA GAACCAGCCC 
CAAGAAGAGG GTGATGCTGG GGATGCCCAA GGCACTTGTG AAGGGGCCAC TGCCCAGGAC 
GAGGGTGCGA CGGGAGGTTC CCAAGCCAAC GGCCACCAGC CATTGAGATC ACTACCTTCA 
GTACGCCAGG ACGTGAGCCG ATACCAGAGG GTGGACGAGG CGCTCCCACC AAACTGGGAG 
GCACGCATTG ACAGCCACGG GAGGATCTTT TATGTGGATC ATGTAAATAG AACCACGACA 
TGGCAGCGGC CGACAGCTCC CCCAGCCCCA CAGATGCTTC AGAGATCCAA CTCCATCCAG 
CAAATGGAGC AGTTGAACCG GCGATATCAG AGTATCCGCA GAACCATGAC TAATGAGAGG 
CCTGAGGAAA ATACCAGTGC TCTTGATGGG GCAGGGGAGG AAGCTGACTT TCACCAAGCC 
AGTGCAGATT TCCGACGGGA GAACGTTCTT CCCCACTCTA CCTCCAGATC CAGGCTCACG 
TTGCTGTTAC AGTCTCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG 
CATTCCAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCATCACTTT GAACGTTACC AACATAACCG GGACCTTGTG 
GGATTCCTCA ACATGTTTGC AAACAAACAG CTGGAGCTGC CGAGGGGCTG GGAAATGAAA 
CATGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAATT CCCGTACCAC CACTTTCATC 
GATCCTCGGC TCCCGCTTCA GAGCAGTAGA CCCACAAGTG CACTGGTTCA TCGGCAGCAC 
TTGACCAGGC AGCGCAGTCA CAGTGCGGGT GAGGTAGGAG AAGATTCCCG ACATGCAGGA 
CCACCTGTTC TTCCCAGGCC ATCCAGTACA TTCAACACAG TCAGTAGGCC ACAGTACCAG 
GACATGGTTC CAGTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGTCA ACCCAATATC 
TTTGAAATTC TACAGGAGCG TCAACCTGAT CTTACCAGGA ATCACTCACT CAGGGAGAAG 
ATTCAGTTTA TCCGAACTGA AGGAACCCCA GGATTGGTGC GTCTTTCAAG TGATGCAGAC 
CTTGTTATGT TGCTGAGTTT ATTTGAAGAA GAGATAATGT CATATGTGCC TCCACATGCC 
TTACTCCACC CCAGCTACTG TCAGTCCCCA CGTGGCTCCC CTGTGTCCTC TCCTCAGAAC 
TCGCCAGGTG TGTCCCAGAT CTCTCACCTG AACTCCAGTT CTCTATTTTC AAGTGTCCAT 
AGAGCAGTTG CCCACCTGAC CAGCTATCAC CTCCAACTCA GTACTCAGCG TGCCAATGCC 
CGGGCTCCAG CCCCTTACAA GCGAGATTTT GAAGCCAAAC TAAGGAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGAAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTACTGG AAGATGCTTT TAATCAAATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTAT ATGTCACATT TGTTGGTGAG GAAGGGCTGG ATTACAGTGG ACCATCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCGT ATTATGGCCT ATTTGAATAT 
TCAGCCAATG ATACATACAC AGTACAAATA AGCCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TTCGGTTCAG TGGTCGGATC CTTGGTCTTG CACTCATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCGTTTTAT AAGGCTCTTC TCAGAATCCT GTGTGACCTG 
AGTGATCTAG AATATCTTGA TGAAGAGTTC CACCAGAGCC TGCAGTGGAT GAAAGATAAC 
GATATCCATG ACATCCTGGA CCTGACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACGGAACGAG AATTGAAGCC AGGGGGTGCC AATATCCCAG TCACAGAGAA GAACAAGAAG 
GAATACATCG AGAGGATGGT GAAGTGGAGA ATTGAGAGGG GTGTTGTACA ACAAACAGAG 
AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAGCTGG AACTGGTCAT TGCGGGCACA GCTGAAATAG ACTTAAGTGA TTGGAGAAAC 
AACACAGAAT ATAGAGGAGG ATACCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA CGAACAGCGA CTAAGGTTGT TACAGTTTGT CACAGGCACG 
TCCAGCATTC CCTACGAAGG ATTTGCATCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACTGCT CTTCCCAGGG CCCATACTTG TTTTAACCGT 
CTGGATCTGC CCCCATACCC ATCCTTCTCC ATGCTTTATG AAAAACTCTT GACAGCAGTT 
GAAGAGACGA GTACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_003406158.2 ) cDNA ORF clone, Loxodonta africana(African savanna elephant) -> XP_003406206.1 Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OLo33735
Clone ID Related Accession (Same CDS sequence)	XM_003406158.2
Accession Version	XM_003406158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1418140800000
Organism	Loxodonta africana(African savanna elephant)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003573423.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Dec 11, 2014 this sequence version replaced XM_003406158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Loxodonta africana Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA ACATCTGCTT TTTGTGCGGC GTCGAAATCC CCAGATGCGC 
TACACACTGA GCCCTGAGAA CCTCCAGAGC CTTGCTGCCC AGAGCTCCAT GCCGGAGAAC 
ATGACCCTCC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAAAG CCGCTCCAGT 
TTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCATAT TTTATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCGGAAGGC ATGGAAGGAG GTGTGTCAGG AAGTTCGCAC 
TCTCGGAAAC TCGTCAGCTT TACACTGTCA GATCTCAGGG CGGTTGGTCT AAAGAAAGGC 
ATGTTCTTCA ATCCTGATCC GTATCTTAAG ATGTCCATCC AGCCAGGTAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACG 
AATCCAATTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TTCTAACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAACGTTT TCTGGGAAAA 
CTAACTATCC CGGTCCAGAG GCTGCTGGAG CGGCAGGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAGAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCT TCCCCAGAAG CTGTGGGCAC AATACTTGGC 
GTCAGTACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG ATGAGGACAT GCCAGGGGGC 
CACCATGACA GCCAAGTGTG TTCCAATGGA CCAACATCTG AGGAAAGTGC TGCTGATGGG 
ACCCCCAAAC ATTCTCTCAG GACTAGCTCC ACTTTGGAAA TAGACACAGA GGAATTAATC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGGCGTCAGG ATTCACTCAA TGATTATTTA 
GATGCTATTG AACATAATGG CCATTCCAGG CCAGGGGCAG CTACTTGCTC GGAGAGGTCC 
ATGGGAGCCT CTCCAAAACT AAGGAGTAGT TTTCCCACGG ACACAAGACT CAACGCGATG 
CTTCATATCG ACTCAGATGA AGAAGATCAC GAGTTTCAGC AAGACCTGGG TTATCCGTCT 
TCCTTGGAGG AGGAAGGCGG ATTAATAATG TTTAGCAGGG CATCCAGAGC TGATGACGAT 
GGGAGCCTGG CATCTCAGAC GAAGCCAGAA GACAACCCAG TTGAGAATGA AGAAGCCTTC 
GCAAATGAAG CTACTTCCTT CGAGGATAAG CCGGAAAATC ATGCAGAGCT TGCAGATAGC 
ACCTTACCCT CAGGCCCATC CCCAGATGAA AACGAAAATG GCCCAGAGTC TCAGCAAAGT 
GCTGACCAGG GCAGCGGTGA GTTGTGTGGT TCTCAAGAGA TGGATCAGCC CACAAGTGGG 
GCAGATACGG GAACTTCCGA TACATCAGGA GGAAGCCGAA GAGCTGTCAG TGAGACTGAA 
TCACTTGATC AGGGGTCTGA GCCTTCCCAG GTGTCCTCTG AAACGGAACC CAGTGACCCT 
GCCAGGACAG AGAGTGTGAG TGAGGCCAGC ACCAGGCCCG AGGGAGAGAG TGACCTGGAA 
GGCGCCGATA GCTCTTGCAA TGAGAGTGTT ACCACGCAGC TGTCCTCGGT GGAGACACGC 
TGCTCGTCTC TTGAGAGTGC ACGGTTTCCA GAAACCCCTG CTTTTTCTTC TCAGGAGGAG 
GAAGATGGAG TCTGTGCAGC AGAGCCCACC AGCAGTGGCC CTGCCGAAGG GCCACAGGAG 
TCCGTTTCCA CTCCTGGTTC TTTACCTGTG GTGCAAGTGC CCAGTGGGGA GGAGGAAGGG 
CCAGAATCAG CTACTTTGCC TGACCAAGAG GAGATGGGAG CGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCCACTGC TGAGAACCAG CCCCAAGAAG AGGGTGATGC TGGGGATGCC 
CAAGGCACTT GTGAAGGGGC CACTGCCCAG GACGAGGGTG CGACGGGAGG TTCCCAAGCC 
AACGGCCACC AGCCATTGAG ATCACTACCT TCAGTACGCC AGGACGTGAG CCGATACCAG 
AGGGTGGACG AGGCGCTCCC ACCAAACTGG GAGGCACGCA TTGACAGCCA CGGGAGGATC 
TTTTATGTGG ATCATGTAAA TAGAACCACG ACATGGCAGC GGCCGACAGC TCCCCCAGCC 
CCACAGATGC TTCAGAGATC CAACTCCATC CAGCAAATGG AGCAGTTGAA CCGGCGATAT 
CAGAGTATCC GCAGAACCAT GACTAATGAG AGGCCTGAGG AAAATACCAG TGCTCTTGAT 
GGGGCAGGGG AGGAAGCTGA CTTTCACCAA GCCAGTGCAG ATTTCCGACG GGAGAACGTT 
CTTCCCCACT CTACCTCCAG ATCCAGGCTC ACGTTGCTGT TACAGTCTCC CCCTGTGAAG 
TTCCTCATCA GCCCAGAGTT CTTCACCGTG CTGCATTCCA ACCCTAGTGC CTACCGCATG 
TTTACAAACA ACACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACCCATCAC 
TTTGAACGTT ACCAACATAA CCGGGACCTT GTGGGATTCC TCAACATGTT TGCAAACAAA 
CAGCTGGAGC TGCCGAGGGG CTGGGAAATG AAACATGATC ATCAGGGCAA GGCATTTTTT 
GTCGACCACA ATTCCCGTAC CACCACTTTC ATCGATCCTC GGCTCCCGCT TCAGAGCAGT 
AGACCCACAA GTGCACTGGT TCATCGGCAG CACTTGACCA GGCAGCGCAG TCACAGTGCG 
GGTGAGGTAG GAGAAGATTC CCGACATGCA GGACCACCTG TTCTTCCCAG GCCATCCAGT 
ACATTCAACA CAGTCAGTAG GCCACAGTAC CAGGACATGG TTCCAGTGGC TTACAATGAC 
AAGATTGTTG CATTTCTACG TCAACCCAAT ATCTTTGAAA TTCTACAGGA GCGTCAACCT 
GATCTTACCA GGAATCACTC ACTCAGGGAG AAGATTCAGT TTATCCGAAC TGAAGGAACC 
CCAGGATTGG TGCGTCTTTC AAGTGATGCA GACCTTGTTA TGTTGCTGAG TTTATTTGAA 
GAAGAGATAA TGTCATATGT GCCTCCACAT GCCTTACTCC ACCCCAGCTA CTGTCAGTCC 
CCACGTGGCT CCCCTGTGTC CTCTCCTCAG AACTCGCCAG GTACTCAGCG TGCCAATGCC 
CGGGCTCCAG CCCCTTACAA GCGAGATTTT GAAGCCAAAC TAAGGAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGAAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTACTGG AAGATGCTTT TAATCAAATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTAT ATGTCACATT TGTTGGTGAG GAAGGGCTGG ATTACAGTGG ACCATCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCGT ATTATGGCCT ATTTGAATAT 
TCAGCCAATG ATACATACAC AGTACAAATA AGCCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TTCGGTTCAG TGGTCGGATC CTTGGTCTTG CACTCATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCGTTTTAT AAGGCTCTTC TCAGAATCCT GTGTGACCTG 
AGTGATCTAG AATATCTTGA TGAAGAGTTC CACCAGAGCC TGCAGTGGAT GAAAGATAAC 
GATATCCATG ACATCCTGGA CCTGACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACGGAACGAG AATTGAAGCC AGGGGGTGCC AATATCCCAG TCACAGAGAA GAACAAGAAG 
GAATACATCG AGAGGATGGT GAAGTGGAGA ATTGAGAGGG GTGTTGTACA ACAAACAGAG 
AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAGCTGG AACTGGTCAT TGCGGGCACA GCTGAAATAG ACTTAAGTGA TTGGAGAAAC 
AACACAGAAT ATAGAGGAGG ATACCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA CGAACAGCGA CTAAGGTTGT TACAGTTTGT CACAGGCACG 
TCCAGCATTC CCTACGAAGG ATTTGCATCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACTGCT CTTCCCAGGG CCCATACTTG TTTTAACCGT 
CTGGATCTGC CCCCATACCC ATCCTTCTCC ATGCTTTATG AAAAACTCTT GACAGCAGTT 
GAAGAGACGA GTACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003406206.1
CDS	183..4892
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGVSGSSHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSVHEDASPEAVGTILGVSTVNGDLGSPSDDEDMPGGHHDSQVCSNGPTSEESAADGTPKHSLRTSSTLEIDTEELISTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGAATCSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDDGSLASQTKPEDNPVENEEAFANEATSFEDKPENHAELADSTLPSGPSPDENENGPESQQSADQGSGELCGSQEMDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGVCAAEPTSSGPAEGPQESVSTPGSLPVVQVPSGEEEGPESATLPDQEEMGAVWQRRGSLEGATAENQPQEEGDAGDAQGTCEGATAQDEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQMLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSALDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_003406158.2
Organism	Loxodonta africana(African savanna elephant)
Definition	Loxodonta africana HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003406158.2

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA ACATCTGCTT TTTGTGCGGC GTCGAAATCC CCAGATGCGC 
TACACACTGA GCCCTGAGAA CCTCCAGAGC CTTGCTGCCC AGAGCTCCAT GCCGGAGAAC 
ATGACCCTCC AGCGGGCCAA CAGTGACACG GACCTGGTGA CTTCTGAAAG CCGCTCCAGT 
TTGACTGCCA GCATGTATGA GTACACGCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGAC TTTATCATAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCATAT TTTATGGAAC CGGAGATAAA AATCTGTTTT 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACAA CCCCCTGCAT CACCGTGAAG 
AACCCTGCTG TGATGATGGG GGCGGAAGGC ATGGAAGGAG GTGTGTCAGG AAGTTCGCAC 
TCTCGGAAAC TCGTCAGCTT TACACTGTCA GATCTCAGGG CGGTTGGTCT AAAGAAAGGC 
ATGTTCTTCA ATCCTGATCC GTATCTTAAG ATGTCCATCC AGCCAGGTAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACG 
AATCCAATTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TTCTAACTGA TGTCTTAGAA 
ATTGAAATTA AAGACAAATT TGCCAAGAGC CGTCCTATCA TCAAACGTTT TCTGGGAAAA 
CTAACTATCC CGGTCCAGAG GCTGCTGGAG CGGCAGGCCA TCGGTGATCA AATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CACGTGAGTG GGTACCTCCA GTTTAGAGTG 
GAGGTTACAT CTTCTGTTCA TGAAGATGCT TCCCCAGAAG CTGTGGGCAC AATACTTGGC 
GTCAGTACTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG ATGAGGACAT GCCAGGGGGC 
CACCATGACA GCCAAGTGTG TTCCAATGGA CCAACATCTG AGGAAAGTGC TGCTGATGGG 
ACCCCCAAAC ATTCTCTCAG GACTAGCTCC ACTTTGGAAA TAGACACAGA GGAATTAATC 
TCTACTTCTT CAAGGACCTC ACCTCCCAGA GGGCGTCAGG ATTCACTCAA TGATTATTTA 
GATGCTATTG AACATAATGG CCATTCCAGG CCAGGGGCAG CTACTTGCTC GGAGAGGTCC 
ATGGGAGCCT CTCCAAAACT AAGGAGTAGT TTTCCCACGG ACACAAGACT CAACGCGATG 
CTTCATATCG ACTCAGATGA AGAAGATCAC GAGTTTCAGC AAGACCTGGG TTATCCGTCT 
TCCTTGGAGG AGGAAGGCGG ATTAATAATG TTTAGCAGGG CATCCAGAGC TGATGACGAT 
GGGAGCCTGG CATCTCAGAC GAAGCCAGAA GACAACCCAG TTGAGAATGA AGAAGCCTTC 
GCAAATGAAG CTACTTCCTT CGAGGATAAG CCGGAAAATC ATGCAGAGCT TGCAGATAGC 
ACCTTACCCT CAGGCCCATC CCCAGATGAA AACGAAAATG GCCCAGAGTC TCAGCAAAGT 
GCTGACCAGG GCAGCGGTGA GTTGTGTGGT TCTCAAGAGA TGGATCAGCC CACAAGTGGG 
GCAGATACGG GAACTTCCGA TACATCAGGA GGAAGCCGAA GAGCTGTCAG TGAGACTGAA 
TCACTTGATC AGGGGTCTGA GCCTTCCCAG GTGTCCTCTG AAACGGAACC CAGTGACCCT 
GCCAGGACAG AGAGTGTGAG TGAGGCCAGC ACCAGGCCCG AGGGAGAGAG TGACCTGGAA 
GGCGCCGATA GCTCTTGCAA TGAGAGTGTT ACCACGCAGC TGTCCTCGGT GGAGACACGC 
TGCTCGTCTC TTGAGAGTGC ACGGTTTCCA GAAACCCCTG CTTTTTCTTC TCAGGAGGAG 
GAAGATGGAG TCTGTGCAGC AGAGCCCACC AGCAGTGGCC CTGCCGAAGG GCCACAGGAG 
TCCGTTTCCA CTCCTGGTTC TTTACCTGTG GTGCAAGTGC CCAGTGGGGA GGAGGAAGGG 
CCAGAATCAG CTACTTTGCC TGACCAAGAG GAGATGGGAG CGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCCACTGC TGAGAACCAG CCCCAAGAAG AGGGTGATGC TGGGGATGCC 
CAAGGCACTT GTGAAGGGGC CACTGCCCAG GACGAGGGTG CGACGGGAGG TTCCCAAGCC 
AACGGCCACC AGCCATTGAG ATCACTACCT TCAGTACGCC AGGACGTGAG CCGATACCAG 
AGGGTGGACG AGGCGCTCCC ACCAAACTGG GAGGCACGCA TTGACAGCCA CGGGAGGATC 
TTTTATGTGG ATCATGTAAA TAGAACCACG ACATGGCAGC GGCCGACAGC TCCCCCAGCC 
CCACAGATGC TTCAGAGATC CAACTCCATC CAGCAAATGG AGCAGTTGAA CCGGCGATAT 
CAGAGTATCC GCAGAACCAT GACTAATGAG AGGCCTGAGG AAAATACCAG TGCTCTTGAT 
GGGGCAGGGG AGGAAGCTGA CTTTCACCAA GCCAGTGCAG ATTTCCGACG GGAGAACGTT 
CTTCCCCACT CTACCTCCAG ATCCAGGCTC ACGTTGCTGT TACAGTCTCC CCCTGTGAAG 
TTCCTCATCA GCCCAGAGTT CTTCACCGTG CTGCATTCCA ACCCTAGTGC CTACCGCATG 
TTTACAAACA ACACGTGTTT GAAGCACATG ATCACCAAAG TCCGGCGGGA CACCCATCAC 
TTTGAACGTT ACCAACATAA CCGGGACCTT GTGGGATTCC TCAACATGTT TGCAAACAAA 
CAGCTGGAGC TGCCGAGGGG CTGGGAAATG AAACATGATC ATCAGGGCAA GGCATTTTTT 
GTCGACCACA ATTCCCGTAC CACCACTTTC ATCGATCCTC GGCTCCCGCT TCAGAGCAGT 
AGACCCACAA GTGCACTGGT TCATCGGCAG CACTTGACCA GGCAGCGCAG TCACAGTGCG 
GGTGAGGTAG GAGAAGATTC CCGACATGCA GGACCACCTG TTCTTCCCAG GCCATCCAGT 
ACATTCAACA CAGTCAGTAG GCCACAGTAC CAGGACATGG TTCCAGTGGC TTACAATGAC 
AAGATTGTTG CATTTCTACG TCAACCCAAT ATCTTTGAAA TTCTACAGGA GCGTCAACCT 
GATCTTACCA GGAATCACTC ACTCAGGGAG AAGATTCAGT TTATCCGAAC TGAAGGAACC 
CCAGGATTGG TGCGTCTTTC AAGTGATGCA GACCTTGTTA TGTTGCTGAG TTTATTTGAA 
GAAGAGATAA TGTCATATGT GCCTCCACAT GCCTTACTCC ACCCCAGCTA CTGTCAGTCC 
CCACGTGGCT CCCCTGTGTC CTCTCCTCAG AACTCGCCAG GTACTCAGCG TGCCAATGCC 
CGGGCTCCAG CCCCTTACAA GCGAGATTTT GAAGCCAAAC TAAGGAACTT TTACAGGAAG 
TTAGAGACTA AAGGATATGG ACAAGGCCCA GGAAAATTAA AGTTAATTAT CAGAAGAGAT 
CACTTACTGG AAGATGCTTT TAATCAAATT ATGGGCTACT CCAGAAAAGA CCTGCAGAGA 
AATAAGCTAT ATGTCACATT TGTTGGTGAG GAAGGGCTGG ATTACAGTGG ACCATCCAGA 
GAGTTTTTCT TCCTGGTATC CAGAGAACTC TTTAACCCGT ATTATGGCCT ATTTGAATAT 
TCAGCCAATG ATACATACAC AGTACAAATA AGCCCCATGT CTGCTTTTGT AGACAATCAC 
CATGAATGGT TTCGGTTCAG TGGTCGGATC CTTGGTCTTG CACTCATTCA CCAGTATTTG 
TTGGATGCCT TCTTCACACG GCCGTTTTAT AAGGCTCTTC TCAGAATCCT GTGTGACCTG 
AGTGATCTAG AATATCTTGA TGAAGAGTTC CACCAGAGCC TGCAGTGGAT GAAAGATAAC 
GATATCCATG ACATCCTGGA CCTGACGTTC ACTGTGAACG AAGAAGTTTT TGGGCAGATT 
ACGGAACGAG AATTGAAGCC AGGGGGTGCC AATATCCCAG TCACAGAGAA GAACAAGAAG 
GAATACATCG AGAGGATGGT GAAGTGGAGA ATTGAGAGGG GTGTTGTACA ACAAACAGAG 
AGCTTAGTGC GTGGCTTCTA TGAGGTGGTG GATGCCAGGC TGGTATCTGT TTTTGATGCG 
AGAGAGCTGG AACTGGTCAT TGCGGGCACA GCTGAAATAG ACTTAAGTGA TTGGAGAAAC 
AACACAGAAT ATAGAGGAGG ATACCATGAC AATCATATTG TAATTCGGTG GTTCTGGGCT 
GCAGTGGAAA GATTCAACAA CGAACAGCGA CTAAGGTTGT TACAGTTTGT CACAGGCACG 
TCCAGCATTC CCTACGAAGG ATTTGCATCT CTCCGAGGGA GTAACGGCCC AAGAAGATTC 
TGTGTGGAGA AATGGGGGAA AATCACTGCT CTTCCCAGGG CCCATACTTG TTTTAACCGT 
CTGGATCTGC CCCCATACCC ATCCTTCTCC ATGCTTTATG AAAAACTCTT GACAGCAGTT 
GAAGAGACGA GTACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW2 cDNA ORF clone, Loxodonta africana(African savanna elephant)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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