LMJF_32_3150 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	LMJF_32_3150
Entrez Gene ID	5656439
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001685589.1	XP_001685641.1	conserved hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LMJF_32_3150 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_32_3150 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb06629	XM_001685589.1 Latest version!	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LMJF_32_3150 ( XM_001685589.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001685641.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OLb06629
Clone ID Related Accession (Same CDS sequence)	XM_001685589.1
Accession Version	XM_001685589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	conserved hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007273). COMPLETENESS: incomplete on both ends.

ATGGCATCCA TGGTACGCCA CTACTCTCTT TCCAATCCTC CGCCGGTCGA GGGCGCGCTG 
GATGGCCGCG AGACAGTGCA CGAGGCGGAG ATACTGGAGG TGCAAGCTCT CCAGGCAGAG 
CGCGATGGCG AATGCGGGCG CGGTGTACTG CTCATGGAGC GCGCGCTGCA GATTCGCTGC 
CACCTGGTGA ACCGACTGCG CGAGAAATGG GCCAGTGCTG CGTCAGGGAC GGGCTCGTCG 
TCGGCCGCCG GCGCTCATCG CGTGTCTGTC TTCGCTAATG CGGATGCGAT GGGTAAAGAA 
GAGCTTCTTT TGGAGTATCA GACCCAATGC GGTGAGCTCT ACGACACGGC CGAGCGCCTT 
GTGGTTCGCT GCAACGGGCT CGCTGTCGAG CACTTTAAGC GAGGCGCATT CTCGGAAGCC 
AGCCCACTGC TCGAGTACGC GATGCAGTTA ACGGAGGATG GGGCGTACCC GCTATGCGAG 
GTCGATGAGC GACGGCGGCA CTTGCGCGGG GTCACGCTCA ACAACCTCGG CTGCATGGAG 
CGCCGCCGCG GCCACTTCTC CGAGGCACTA CAGTACATGA AGTCGTCGAT GGAGATGACC 
GGGGTCGAGT CACCTGTGGC ATACATGAAC ACCAGCGCTA TTCTTATCCA GCTGCGGCTC 
AGCGACGAGG CGGTGCGCAT GGCGGAGCGC TCCATCGAGC TACTTTACCA AACACCCGAG 
GACCCGTCAC TGCTGGCAGT GGCGCATCAC AACCTGGCCA TGGCACTCGA GCCGGTTGAT 
CCGGCACGGT GCTTGGAGGA GTATGCGCTA GCATACCGGA CGGCCTGCTC CACGCTGGGC 
CCGGAGAGCG AGACGAGTCT CTGTATTCAG CGCAGCTGGA GGCGGTATGA GGCGACGCGC 
GTGCCCCCGG CAACGGGGGC GTTTTTCATG GGCGGTGATG GCGCCGCTGC ATCTCGGCTG 
CGTAGGCTCG TTGCTGCCGC GCCAACCAGG CGTGCATACA CTGCACCGCA TCACTTGCCT 
GCAACGGTGC CGCGCACGCA CACCGGCAAG AGCGGTGCTG GCTCCCCGAT GGACGTGATA 
GAGCTCTTTC CGCACCCTTT TCTGCCCAGT GCGTCCGTCT CGTCCGCGCC GACAAAGACG 
CCGCGGGATG TGACGCCGAT CTACCGCCCC GCAGTGGTGC CCCGTCGGCC TCTTGCCCCT 
GGACCGCCGC CAGGCGTAAG GGCGACCTCA TCCAGTCGCA GTCGTGAGCC ACAGTCGCAG 
CCGCGCCACC AGCAGCAAGA GCAGCACCGA CAGCCGCGGC GTCAGTATGG GACGGAGAAA 
GTGATGCGCT CCTCGCCACC AGCCTCCCGT ACTGCGGTGG CGGCCCCACG ACCGCCACCC 
ACCAACCGGG CCAAGGAGGC TGCGGTGGCA GCCGGCAGCG GCCGCGTCGC CGCTTCAATG 
ACGCCGTCGC CCCCGCCCTC TCGCCAGAGG CAAGCGACCG CCCAGCGGCG CTACTCTCCT 
ACGACACAAG CGCAAGAGTC GTCACGGCAG TCACCGCCGC GTACAGGCAA CCGTCAGCAA 
CGCTTGTCGG TGCAAAAGAG TACGGCGGAT GCGTGCCAAA CCGCTAGCGG GTCACGAAAG 
CGGGAGAAGA GCACATTGCA CTACGCCAAT GACCGGCTCA CAACGCAGGC ATCGTCCACC 
GCGCTGTCGT CTTCCGCTGC GCAGTCACCA GGACGCGGGC CACGTCCCCC CATAAAGGAG 
AGCAGCAGCA GCAACCATTC TCTCCCCATG CCACTACCGC GAAACCTCCC GCCATTGCAG 
ACCGCCGTCG CTGCGGAGCC GCCTGCGGTG GCGCCGAATT CGTCGTACTC CCGCTCGTTG 
CACGACGCCA AGGCGAATGC GTCGCTTCCG TCCACCTCAG CGTCGCTTTC CCTCACGTCG 
GATCCGCTGA CATTTCTGCA GAATCGACTC GAAATTCTCC TTCAAGACGA AGAAGAGCTA 
GAGCACAAGT ACGTTCAAGC GACAGTGATT CAGCGGTACT ACCGCGGCCA CCTGGCCCAT 
CGTCGTGTGG CAGCGCTCCG AGCTACCCGC GCGAGTTATG CGCGGCTCGC CCAGCTGCGC 
TGCCATATTG CAGCACGTTG CATTCAGCGA GCGTTTCGGC GCCACCGCCG TCACAGCCGC 
TACCCTCTTG CAGCTGGTCA GCTGGGCCGC TACGCAGCAG CCGGAGGCCG ACGCGGTGCT 
CAGCACCAAG CGGCTACACT GCTGCAGCGA ATTGCGCGCG GTTGGCTGGC GCGGCGTCAC 
TACGCGCAGC TGCGCAAGTA CGCATGTGAG AGTCCAGCTG CCGCGACTCG CATCCAGCGC 
TGGATTCGCG CACTGCAAGC GCGACGCCAC TACGCAGTCT TGCTCGCCGC AAAGGCGGAG 
CAGGATGCCG TGGCGCTGGA GCACGAACGT CGTCAGTACG CGGCGACTCG CATTCAAGGC 
CAATGGCTCA CCCACCTGCA GCGCAGAGCA TGCCAGGCGG CCCTGCGCCA CCGTATGATG 
GCCCGTGCCG CCGAGGATGC CCGGTGCCGC ATGAGGGCGG CTATCATCAT CCAGTCAGCA 
TGGCGAGGGT ACCAGGCGCG CCGGCTCTAT CAAGACACGT ACTCGCGCAC CTTCCAGCTG 
CGTACGGCCC GGCTGGAGTA CCAGCGCCGT CGTCAGGCTG CTGTGCGACT TCAGGCATTT 
GGGCGGATGC TCATAGCCAG GCAGCACGCA ACGCCGCTGC TCACGGTCGC CCGCTTCCGA 
GCAGCGGCTG AGATTCGAAC CCGCAGCCAT GAAGGGCAGG CCGCTATTAA GATTCAGTGT 
GCCTACCGCC GCCACGTGGC TCATCGAGTG TTTGTCACCA AGCGTGCTGC GCGCAAGAAC 
CAGATTAGTC AGGACCTAGT GCAGGTGCAC ACCACGACCG TGCAACGTGC GGGACGAGGC 
TACATGGCGC GGAAAGACGT AGGGACCAAG CTGTCAGTGC TGCAGGCTGA GGCAGAGCGG 
TACGAGCGTC GTCTGCTGGC TTTGAAGCAG AAAGAGCTGG AGGCTGCACG CGCTGTCGCG 
CTGGCAGTTC AGCTCAGTAT CTCCGAACTA CGCGAATGCG AGGGTGAGCA GCGGCGTATC 
CTTGCGCGCG CGGAGCAGCA GCAGTGGCAC GACATGCTCA CACAGTGCAA CGCACTCTTA 
GCGGCGACAA AGGAGACTGA GGTAGAGCAA GAGGCGATGA AGGTGATTTC ATCAGAGGCC 
TTACATGACT TTTCCCGCAT CATCAAGGCG AAGAAGCAGC GAGCCCATCG CCAAGAGGCG 
CGCGACGCCT ACCTGGACCA GTGCGCGGCA GAGGATGCCG AGGCGCATAA GGAGGCGAGC 
GCGCGCGTGG TAACTGACTT CATGCGTAGC GCCGCCGCGC GCAGCACCTT GCGTAGCTTA 
GTCAAGACGA TGAGCTCGTA CAACGACGCT CGTGCGATGC AGGAGGAGGA TGATAAGACG 
CCTGCGGCTG CGACGACGGA TGGGGCAGCC CCGAAGCGCC CGCTGACGGC GGAGCAGATT 
GCGGCTGAGC GTGCTGAGCT GGACCGGCTG GAGCGCGAGG AGCTGCTGGC CTCTGAGGCC 
GCGGCGGCCC CGAAGCGCCC GCTGACGGCG GAGCAGATTG CGGCTGAGCG TGCTGAGCTG 
GACCGGCTGG AGCGCGAGGA GCTGCTGGCC TCTGAGGCCG CGGCGGCCCC GAAGCGCCCG 
CTGACGGCGG AGCAGATTGC GGCTGAGCGT GCTGAGCTGG ACCGGCTGGA GCGCGAGGAG 
CTGCTGGCCT CTGAGGCCGC GGCGGCCCCG AAGCGCCCGC TGACGGCGGA ACAGATTGCG 
GCTGAGCGTG CTGAGCTGGA CCGGCTGGAG CGCGAGGAGC TGCTGGCCTC TGAGGCCGCG 
GCGGCCCCGA AGCGCCCGCT GACGGCGGAG CAGATTGCGG CTGAGCGTGC TGAGCTGGAC 
CGGCTGGAGC GCGAGGAGCT GCTGGCCTCT GAGGCCGCGG CGGCCCCGAA GCGCCCGCTG 
ACGGCGGAAC AGATTGCGGC TGAGCGTGCT GAGCTGGACC GGCTGGAGCG CGAGGAGCTG 
CTGGCCTCTG AGGCCGCGGC GGCCCCGAAG CGCCCGCTGA CGGCGGAGCA GATTGCGGCT 
GAGCGTGCTG AGCTGGACCG GCTGGAGCGC GAGGAGCTGC TGGCCTCTGA GGCCGCGGCG 
GCCCCGAAGC GCCCGCTGAC GGCGGAGCAG ATTGCGGCTG AGCGTGCTGA GCTGGACCGG 
CTGGAGCGCG AGGAGCTGCT GGCCTCTGAG GCCGCGGCGG CCCCGAAGCG CCCGCTGACG 
GCGGAGCAGA TTGCGGCTGA GCGTGCTGAG CTGGACCGGT CGGAGCGCGA GGAGCTGGTA 
TGTAAGAGAG ATACCACAAG CGCGTACGAG GAAGAGGGAC CTCCGGCTGC TGATGAGTCG 
TTCACTTCGG CAGATGCATC TATTAGTGCC AGTGCTTTGG TAGATGTGAT GCTTGCTCGT 
CTGAAGGCCA TCTGGCAGGC CGAGGTGGCG GCAGAGAAGC AGCGCCGCCA CCAACGCGAG 
GAGCTACATT GGGCTATCTT CGAACACAGG CAGCAGCGTA TCGCAGCCGC CGAAGAGGGA 
GAGGACCTTG CTCCCTATCG TATTAACCGG GTGCACCTGC AGATGCCCTC CCCTGCTGCG 
CCGAAGAAGG CCCACCCGTC GCCTGCAGAG TTGTCCACAG CTATGGGGAA TAGGTACCGT 
CACGAGCGCG CTGCACAGGA GCTTGCTGCT GTACAGTGCA TCGAGCGCTG CTTCATTGGC 
TGGGGTGCCC GAAAGCACCT GAAGGCGTTG CTACGTGTGC TGGATGAGTA TCTCAGTGCC 
CTGCAGGACT TTGACCGTCA TGAGGGACCG GTGCTTACCT ACGCACGGCT ACGCGAAGTA 
CTAGCGCGTT ACCCAGATTT GCGAACGCGT GTTTCGCAGC TGCCCGTTTC CCTATCCCCT 
CCTGAGCGTG CCGCCATTGA AGACGGCAAC TCATCAGCTT CACCAGCAAT ATCACCAACC 
TCGAGGAAAA CACTGCAAGC CTTTTCCTGC ATCATCGAGG CGAAGAAGCA GCGAGCCCAT 
CGCCAAGAGG CGCGCGACGC CTACCTGGAC CAGTGCGCGG CAGAGGATGC CGAGGCGCAT 
AAGGAGGCGA GCGCGCGCGT GGTAACTGAC TTCATGCGTA GCGCCGCCGC GCGCAGCACC 
TTGCGTAGCT TAGTCAAGAC GATGAGCTCG TACAACGACG CTCGTGCGAT GCAGGAGGAG 
GATGATAAGA CGCCTGCGGC TGCGACGACG GATGGGGCAG CCCCGAAGCG CCCGCTGACG 
GCGGAGCAGA TTGCGGCTGA GCGTGCTGAG CTGGACCGGC TGGAGCGCGA GGAGCTGCTG 
GCCTCTGAGG CCGCGGCGGC CCCGAAGCGC CCGCTGACGG CGGAACAGAT TGCGGCTGAG 
CGTGCTGAGC TGGACCGGCT GGAGCGCGAG GAGCTGCTGG CCTCTGAGGC CGCGGCGGCC 
CCGAAGCGCC CGCTGACGGC GGAGCAGATT GCGGCTGAGC GTGCTGAGCT GGACCGGCTG 
GAGCGCGAGG AGCTGCTGGC CTCTGAGGCC GCGGCGGTCC CGAAGCGCCC GCTGACGGCG 
GAACAGATTG CGGCTGAGCG TGCTGAGCTG GACCGGCTGG AGCGCGAGGA GCTGCTGGCC 
TCTGAGGCCG CGGCGGCCCC GAAGCGCCCG CTGACGGCGG AGCAGATTGC GGCTGAGCGT 
GCTGAGCGGG ACCGGCTGGA GCGCGAGGAG CTGCTGGCCT CTGAGGCCGC GGCGGCCCCG 
AAGCGCCCGC TGACGGCGGA ACAGATTGCG GCTGAGCGTG CTGAGCTGGA CCGGTCGGAG 
CGCGAGGAGA GAGATGTCGA TGCAGGGGAG GTTAAGGCGT CGCAGCCCTT GGGGTTCGAG 
CAGCTGCTCG CAGAAGGTGC AGCAGTTGGC CATCTTGAAC GCTGGGAACA GATAAGCTTC 
GATGGCGGAG CGAGACATCA TCCGCGACGA CTGACCGAAC AGCAGGTTGT GGTGGAGCGC 
TTGGCCCTGG ACCAGCTGGA CGACCTAGAG CAGGTGGCAA TGGAGCGCGG AAGGCTGGGC 
CGACTCAGCT GCGTGGAGCA CAACGTGTTG AGTGCAGCTG CTCTTCTTGC ACAGAGGGTG 
CAGGAGGAGC ATTGCAGCGC CGATTCTGCG TACACCTCGA CGTCGTCATC GGTGAGGCCA 
TTCGAGCCAC GGCAGCGGGA AGGCCTCACC AGCAGCGAGG CGGGTGGTCG CTTGTGGGTG 
TTTGCCAAGA CCATCCGAGA CAAGAAGGAC CTGCGCCAAC GGCAAGCTGC CCGGGACGCT 
TATCTGCAAG AGATGCACAA TGAAAATGCC ATTGATACCG ACGAAGAGGA GAAGCAGCAG 
GAGGAGCGCC CACCGACGCC CTCTTCGGGC GCAATTACGT GGCGGCGCTG CAGCAACGAG 
GTTAGCGGCG ACGATAGAGA GATGATGAGC CAGGAGGCGT TGCACCAGCT GTACCATCAA 
GAGCGCCGAT CCTTTGCCTC CTCGCTTGTA GGCGGCTTTG TCGATATTAT CAAGGCGAAG 
AAGCAGCGAG CCCATCGCCA AGAGGCGCGC GACGCCTACC TGGACCAGTG CGCGGCAGAG 
GATGCCGAGG CGCATAAGGA GGCGAGCGCG CGCGTGGTAA CTGACTTCAT GCGTAGCGCC 
GCCGCGCGCA GCACCTTGTG TAGCCTAGTC AAGACGATGA GCTCGTACAA CGACGCTCGT 
GCGATGCAGG AGGAGGATGA TAAGACGCCT GCGGCTGCGA CGACGGATGA TGGCAGCGGT 
GAGGGTGCGT ACGCAACCCC TGCCGAGTCG TGTGAGCACT CCCCTTTGTC TGCGGCTCTC 
AGAATCCAGC GCTTCTTCAG GGGCGTGCAG GCAAGGCGTC GAGTGCGAGT ACTGCAAGAG 
GCACACCGTG CCTACCTCGA GGAGGAGCTT GCAGTAGAGG AGCTACTCTA CTCAGCGGCG 
GTTGCCATTC AGTGCTTGTA CCGCGGCCAT CTGGCGCGTC GGCAGGCGGC CATGATCATT 
GTGCAGCGTG ATCTCTACCT GTTGTCACTC CAGGAAGAGG ATTGGTTAGG TGGGGATTCG 
AAGCGCGGAG AAGGGAGTGA TGCGTCCTTG CCAGCAGCTC TATCGTTTTT TGCTTCCTCA 
CCAAAGAGTG TCGCAGGCGG CGGAGCCAAG GCAGCGGCGA CTCGCCCCTT TACCCCACGC 
CGCTCTTCCT CGTCATCCTA TACTGGTCTG CGCGAAGTTG CCAGCACAGC CATTGTGACC 
GAGGTGAATG AGGCGGACGA GGCAGCGCTG GACCAGCTTT TGCATCGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001685641.1
CDS	1..7374
Translation	MASMVRHYSLSNPPPVEGALDGRETVHEAEILEVQALQAERDGECGRGVLLMERALQIRCHLVNRLREKWASAASGTGSSSAAGAHRVSVFANADAMGKEELLLEYQTQCGELYDTAERLVVRCNGLAVEHFKRGAFSEASPLLEYAMQLTEDGAYPLCEVDERRRHLRGVTLNNLGCMERRRGHFSEALQYMKSSMEMTGVESPVAYMNTSAILIQLRLSDEAVRMAERSIELLYQTPEDPSLLAVAHHNLAMALEPVDPARCLEEYALAYRTACSTLGPESETSLCIQRSWRRYEATRVPPATGAFFMGGDGAAASRLRRLVAAAPTRRAYTAPHHLPATVPRTHTGKSGAGSPMDVIELFPHPFLPSASVSSAPTKTPRDVTPIYRPAVVPRRPLAPGPPPGVRATSSSRSREPQSQPRHQQQEQHRQPRRQYGTEKVMRSSPPASRTAVAAPRPPPTNRAKEAAVAAGSGRVAASMTPSPPPSRQRQATAQRRYSPTTQAQESSRQSPPRTGNRQQRLSVQKSTADACQTASGSRKREKSTLHYANDRLTTQASSTALSSSAAQSPGRGPRPPIKESSSSNHSLPMPLPRNLPPLQTAVAAEPPAVAPNSSYSRSLHDAKANASLPSTSASLSLTSDPLTFLQNRLEILLQDEEELEHKYVQATVIQRYYRGHLAHRRVAALRATRASYARLAQLRCHIAARCIQRAFRRHRRHSRYPLAAGQLGRYAAAGGRRGAQHQAATLLQRIARGWLARRHYAQLRKYACESPAAATRIQRWIRALQARRHYAVLLAAKAEQDAVALEHERRQYAATRIQGQWLTHLQRRACQAALRHRMMARAAEDARCRMRAAIIIQSAWRGYQARRLYQDTYSRTFQLRTARLEYQRRRQAAVRLQAFGRMLIARQHATPLLTVARFRAAAEIRTRSHEGQAAIKIQCAYRRHVAHRVFVTKRAARKNQISQDLVQVHTTTVQRAGRGYMARKDVGTKLSVLQAEAERYERRLLALKQKELEAARAVALAVQLSISELRECEGEQRRILARAEQQQWHDMLTQCNALLAATKETEVEQEAMKVISSEALHDFSRIIKAKKQRAHRQEARDAYLDQCAAEDAEAHKEASARVVTDFMRSAAARSTLRSLVKTMSSYNDARAMQEEDDKTPAAATTDGAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRSEREELVCKRDTTSAYEEEGPPAADESFTSADASISASALVDVMLARLKAIWQAEVAAEKQRRHQREELHWAIFEHRQQRIAAAEEGEDLAPYRINRVHLQMPSPAAPKKAHPSPAELSTAMGNRYRHERAAQELAAVQCIERCFIGWGARKHLKALLRVLDEYLSALQDFDRHEGPVLTYARLREVLARYPDLRTRVSQLPVSLSPPERAAIEDGNSSASPAISPTSRKTLQAFSCIIEAKKQRAHRQEARDAYLDQCAAEDAEAHKEASARVVTDFMRSAAARSTLRSLVKTMSSYNDARAMQEEDDKTPAAATTDGAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRLEREELLASEAAAVPKRPLTAEQIAAERAELDRLEREELLASEAAAAPKRPLTAEQIAAERAERDRLEREELLASEAAAAPKRPLTAEQIAAERAELDRSEREERDVDAGEVKASQPLGFEQLLAEGAAVGHLERWEQISFDGGARHHPRRLTEQQVVVERLALDQLDDLEQVAMERGRLGRLSCVEHNVLSAAALLAQRVQEEHCSADSAYTSTSSSVRPFEPRQREGLTSSEAGGRLWVFAKTIRDKKDLRQRQAARDAYLQEMHNENAIDTDEEEKQQEERPPTPSSGAITWRRCSNEVSGDDREMMSQEALHQLYHQERRSFASSLVGGFVDIIKAKKQRAHRQEARDAYLDQCAAEDAEAHKEASARVVTDFMRSAAARSTLCSLVKTMSSYNDARAMQEEDDKTPAAATTDDGSGEGAYATPAESCEHSPLSAALRIQRFFRGVQARRRVRVLQEAHRAYLEEELAVEELLYSAAVAIQCLYRGHLARRQAAMIIVQRDLYLLSLQEEDWLGGDSKRGEGSDASLPAALSFFASSPKSVAGGGAKAAATRPFTPRRSSSSSYTGLREVASTAIVTEVNEADEAALDQLLHRP

Target ORF information:

RefSeq Version	XM_001685589.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001685589.1

ORF Insert Sequence:

ATGGCATCCA TGGTACGCCA CTACTCTCTT TCCAATCCTC CGCCGGTCGA GGGCGCGCTG 
GATGGCCGCG AGACAGTGCA CGAGGCGGAG ATACTGGAGG TGCAAGCTCT CCAGGCAGAG 
CGCGATGGCG AATGCGGGCG CGGTGTACTG CTCATGGAGC GCGCGCTGCA GATTCGCTGC 
CACCTGGTGA ACCGACTGCG CGAGAAATGG GCCAGTGCTG CGTCAGGGAC GGGCTCGTCG 
TCGGCCGCCG GCGCTCATCG CGTGTCTGTC TTCGCTAATG CGGATGCGAT GGGTAAAGAA 
GAGCTTCTTT TGGAGTATCA GACCCAATGC GGTGAGCTCT ACGACACGGC CGAGCGCCTT 
GTGGTTCGCT GCAACGGGCT CGCTGTCGAG CACTTTAAGC GAGGCGCATT CTCGGAAGCC 
AGCCCACTGC TCGAGTACGC GATGCAGTTA ACGGAGGATG GGGCGTACCC GCTATGCGAG 
GTCGATGAGC GACGGCGGCA CTTGCGCGGG GTCACGCTCA ACAACCTCGG CTGCATGGAG 
CGCCGCCGCG GCCACTTCTC CGAGGCACTA CAGTACATGA AGTCGTCGAT GGAGATGACC 
GGGGTCGAGT CACCTGTGGC ATACATGAAC ACCAGCGCTA TTCTTATCCA GCTGCGGCTC 
AGCGACGAGG CGGTGCGCAT GGCGGAGCGC TCCATCGAGC TACTTTACCA AACACCCGAG 
GACCCGTCAC TGCTGGCAGT GGCGCATCAC AACCTGGCCA TGGCACTCGA GCCGGTTGAT 
CCGGCACGGT GCTTGGAGGA GTATGCGCTA GCATACCGGA CGGCCTGCTC CACGCTGGGC 
CCGGAGAGCG AGACGAGTCT CTGTATTCAG CGCAGCTGGA GGCGGTATGA GGCGACGCGC 
GTGCCCCCGG CAACGGGGGC GTTTTTCATG GGCGGTGATG GCGCCGCTGC ATCTCGGCTG 
CGTAGGCTCG TTGCTGCCGC GCCAACCAGG CGTGCATACA CTGCACCGCA TCACTTGCCT 
GCAACGGTGC CGCGCACGCA CACCGGCAAG AGCGGTGCTG GCTCCCCGAT GGACGTGATA 
GAGCTCTTTC CGCACCCTTT TCTGCCCAGT GCGTCCGTCT CGTCCGCGCC GACAAAGACG 
CCGCGGGATG TGACGCCGAT CTACCGCCCC GCAGTGGTGC CCCGTCGGCC TCTTGCCCCT 
GGACCGCCGC CAGGCGTAAG GGCGACCTCA TCCAGTCGCA GTCGTGAGCC ACAGTCGCAG 
CCGCGCCACC AGCAGCAAGA GCAGCACCGA CAGCCGCGGC GTCAGTATGG GACGGAGAAA 
GTGATGCGCT CCTCGCCACC AGCCTCCCGT ACTGCGGTGG CGGCCCCACG ACCGCCACCC 
ACCAACCGGG CCAAGGAGGC TGCGGTGGCA GCCGGCAGCG GCCGCGTCGC CGCTTCAATG 
ACGCCGTCGC CCCCGCCCTC TCGCCAGAGG CAAGCGACCG CCCAGCGGCG CTACTCTCCT 
ACGACACAAG CGCAAGAGTC GTCACGGCAG TCACCGCCGC GTACAGGCAA CCGTCAGCAA 
CGCTTGTCGG TGCAAAAGAG TACGGCGGAT GCGTGCCAAA CCGCTAGCGG GTCACGAAAG 
CGGGAGAAGA GCACATTGCA CTACGCCAAT GACCGGCTCA CAACGCAGGC ATCGTCCACC 
GCGCTGTCGT CTTCCGCTGC GCAGTCACCA GGACGCGGGC CACGTCCCCC CATAAAGGAG 
AGCAGCAGCA GCAACCATTC TCTCCCCATG CCACTACCGC GAAACCTCCC GCCATTGCAG 
ACCGCCGTCG CTGCGGAGCC GCCTGCGGTG GCGCCGAATT CGTCGTACTC CCGCTCGTTG 
CACGACGCCA AGGCGAATGC GTCGCTTCCG TCCACCTCAG CGTCGCTTTC CCTCACGTCG 
GATCCGCTGA CATTTCTGCA GAATCGACTC GAAATTCTCC TTCAAGACGA AGAAGAGCTA 
GAGCACAAGT ACGTTCAAGC GACAGTGATT CAGCGGTACT ACCGCGGCCA CCTGGCCCAT 
CGTCGTGTGG CAGCGCTCCG AGCTACCCGC GCGAGTTATG CGCGGCTCGC CCAGCTGCGC 
TGCCATATTG CAGCACGTTG CATTCAGCGA GCGTTTCGGC GCCACCGCCG TCACAGCCGC 
TACCCTCTTG CAGCTGGTCA GCTGGGCCGC TACGCAGCAG CCGGAGGCCG ACGCGGTGCT 
CAGCACCAAG CGGCTACACT GCTGCAGCGA ATTGCGCGCG GTTGGCTGGC GCGGCGTCAC 
TACGCGCAGC TGCGCAAGTA CGCATGTGAG AGTCCAGCTG CCGCGACTCG CATCCAGCGC 
TGGATTCGCG CACTGCAAGC GCGACGCCAC TACGCAGTCT TGCTCGCCGC AAAGGCGGAG 
CAGGATGCCG TGGCGCTGGA GCACGAACGT CGTCAGTACG CGGCGACTCG CATTCAAGGC 
CAATGGCTCA CCCACCTGCA GCGCAGAGCA TGCCAGGCGG CCCTGCGCCA CCGTATGATG 
GCCCGTGCCG CCGAGGATGC CCGGTGCCGC ATGAGGGCGG CTATCATCAT CCAGTCAGCA 
TGGCGAGGGT ACCAGGCGCG CCGGCTCTAT CAAGACACGT ACTCGCGCAC CTTCCAGCTG 
CGTACGGCCC GGCTGGAGTA CCAGCGCCGT CGTCAGGCTG CTGTGCGACT TCAGGCATTT 
GGGCGGATGC TCATAGCCAG GCAGCACGCA ACGCCGCTGC TCACGGTCGC CCGCTTCCGA 
GCAGCGGCTG AGATTCGAAC CCGCAGCCAT GAAGGGCAGG CCGCTATTAA GATTCAGTGT 
GCCTACCGCC GCCACGTGGC TCATCGAGTG TTTGTCACCA AGCGTGCTGC GCGCAAGAAC 
CAGATTAGTC AGGACCTAGT GCAGGTGCAC ACCACGACCG TGCAACGTGC GGGACGAGGC 
TACATGGCGC GGAAAGACGT AGGGACCAAG CTGTCAGTGC TGCAGGCTGA GGCAGAGCGG 
TACGAGCGTC GTCTGCTGGC TTTGAAGCAG AAAGAGCTGG AGGCTGCACG CGCTGTCGCG 
CTGGCAGTTC AGCTCAGTAT CTCCGAACTA CGCGAATGCG AGGGTGAGCA GCGGCGTATC 
CTTGCGCGCG CGGAGCAGCA GCAGTGGCAC GACATGCTCA CACAGTGCAA CGCACTCTTA 
GCGGCGACAA AGGAGACTGA GGTAGAGCAA GAGGCGATGA AGGTGATTTC ATCAGAGGCC 
TTACATGACT TTTCCCGCAT CATCAAGGCG AAGAAGCAGC GAGCCCATCG CCAAGAGGCG 
CGCGACGCCT ACCTGGACCA GTGCGCGGCA GAGGATGCCG AGGCGCATAA GGAGGCGAGC 
GCGCGCGTGG TAACTGACTT CATGCGTAGC GCCGCCGCGC GCAGCACCTT GCGTAGCTTA 
GTCAAGACGA TGAGCTCGTA CAACGACGCT CGTGCGATGC AGGAGGAGGA TGATAAGACG 
CCTGCGGCTG CGACGACGGA TGGGGCAGCC CCGAAGCGCC CGCTGACGGC GGAGCAGATT 
GCGGCTGAGC GTGCTGAGCT GGACCGGCTG GAGCGCGAGG AGCTGCTGGC CTCTGAGGCC 
GCGGCGGCCC CGAAGCGCCC GCTGACGGCG GAGCAGATTG CGGCTGAGCG TGCTGAGCTG 
GACCGGCTGG AGCGCGAGGA GCTGCTGGCC TCTGAGGCCG CGGCGGCCCC GAAGCGCCCG 
CTGACGGCGG AGCAGATTGC GGCTGAGCGT GCTGAGCTGG ACCGGCTGGA GCGCGAGGAG 
CTGCTGGCCT CTGAGGCCGC GGCGGCCCCG AAGCGCCCGC TGACGGCGGA ACAGATTGCG 
GCTGAGCGTG CTGAGCTGGA CCGGCTGGAG CGCGAGGAGC TGCTGGCCTC TGAGGCCGCG 
GCGGCCCCGA AGCGCCCGCT GACGGCGGAG CAGATTGCGG CTGAGCGTGC TGAGCTGGAC 
CGGCTGGAGC GCGAGGAGCT GCTGGCCTCT GAGGCCGCGG CGGCCCCGAA GCGCCCGCTG 
ACGGCGGAAC AGATTGCGGC TGAGCGTGCT GAGCTGGACC GGCTGGAGCG CGAGGAGCTG 
CTGGCCTCTG AGGCCGCGGC GGCCCCGAAG CGCCCGCTGA CGGCGGAGCA GATTGCGGCT 
GAGCGTGCTG AGCTGGACCG GCTGGAGCGC GAGGAGCTGC TGGCCTCTGA GGCCGCGGCG 
GCCCCGAAGC GCCCGCTGAC GGCGGAGCAG ATTGCGGCTG AGCGTGCTGA GCTGGACCGG 
CTGGAGCGCG AGGAGCTGCT GGCCTCTGAG GCCGCGGCGG CCCCGAAGCG CCCGCTGACG 
GCGGAGCAGA TTGCGGCTGA GCGTGCTGAG CTGGACCGGT CGGAGCGCGA GGAGCTGGTA 
TGTAAGAGAG ATACCACAAG CGCGTACGAG GAAGAGGGAC CTCCGGCTGC TGATGAGTCG 
TTCACTTCGG CAGATGCATC TATTAGTGCC AGTGCTTTGG TAGATGTGAT GCTTGCTCGT 
CTGAAGGCCA TCTGGCAGGC CGAGGTGGCG GCAGAGAAGC AGCGCCGCCA CCAACGCGAG 
GAGCTACATT GGGCTATCTT CGAACACAGG CAGCAGCGTA TCGCAGCCGC CGAAGAGGGA 
GAGGACCTTG CTCCCTATCG TATTAACCGG GTGCACCTGC AGATGCCCTC CCCTGCTGCG 
CCGAAGAAGG CCCACCCGTC GCCTGCAGAG TTGTCCACAG CTATGGGGAA TAGGTACCGT 
CACGAGCGCG CTGCACAGGA GCTTGCTGCT GTACAGTGCA TCGAGCGCTG CTTCATTGGC 
TGGGGTGCCC GAAAGCACCT GAAGGCGTTG CTACGTGTGC TGGATGAGTA TCTCAGTGCC 
CTGCAGGACT TTGACCGTCA TGAGGGACCG GTGCTTACCT ACGCACGGCT ACGCGAAGTA 
CTAGCGCGTT ACCCAGATTT GCGAACGCGT GTTTCGCAGC TGCCCGTTTC CCTATCCCCT 
CCTGAGCGTG CCGCCATTGA AGACGGCAAC TCATCAGCTT CACCAGCAAT ATCACCAACC 
TCGAGGAAAA CACTGCAAGC CTTTTCCTGC ATCATCGAGG CGAAGAAGCA GCGAGCCCAT 
CGCCAAGAGG CGCGCGACGC CTACCTGGAC CAGTGCGCGG CAGAGGATGC CGAGGCGCAT 
AAGGAGGCGA GCGCGCGCGT GGTAACTGAC TTCATGCGTA GCGCCGCCGC GCGCAGCACC 
TTGCGTAGCT TAGTCAAGAC GATGAGCTCG TACAACGACG CTCGTGCGAT GCAGGAGGAG 
GATGATAAGA CGCCTGCGGC TGCGACGACG GATGGGGCAG CCCCGAAGCG CCCGCTGACG 
GCGGAGCAGA TTGCGGCTGA GCGTGCTGAG CTGGACCGGC TGGAGCGCGA GGAGCTGCTG 
GCCTCTGAGG CCGCGGCGGC CCCGAAGCGC CCGCTGACGG CGGAACAGAT TGCGGCTGAG 
CGTGCTGAGC TGGACCGGCT GGAGCGCGAG GAGCTGCTGG CCTCTGAGGC CGCGGCGGCC 
CCGAAGCGCC CGCTGACGGC GGAGCAGATT GCGGCTGAGC GTGCTGAGCT GGACCGGCTG 
GAGCGCGAGG AGCTGCTGGC CTCTGAGGCC GCGGCGGTCC CGAAGCGCCC GCTGACGGCG 
GAACAGATTG CGGCTGAGCG TGCTGAGCTG GACCGGCTGG AGCGCGAGGA GCTGCTGGCC 
TCTGAGGCCG CGGCGGCCCC GAAGCGCCCG CTGACGGCGG AGCAGATTGC GGCTGAGCGT 
GCTGAGCGGG ACCGGCTGGA GCGCGAGGAG CTGCTGGCCT CTGAGGCCGC GGCGGCCCCG 
AAGCGCCCGC TGACGGCGGA ACAGATTGCG GCTGAGCGTG CTGAGCTGGA CCGGTCGGAG 
CGCGAGGAGA GAGATGTCGA TGCAGGGGAG GTTAAGGCGT CGCAGCCCTT GGGGTTCGAG 
CAGCTGCTCG CAGAAGGTGC AGCAGTTGGC CATCTTGAAC GCTGGGAACA GATAAGCTTC 
GATGGCGGAG CGAGACATCA TCCGCGACGA CTGACCGAAC AGCAGGTTGT GGTGGAGCGC 
TTGGCCCTGG ACCAGCTGGA CGACCTAGAG CAGGTGGCAA TGGAGCGCGG AAGGCTGGGC 
CGACTCAGCT GCGTGGAGCA CAACGTGTTG AGTGCAGCTG CTCTTCTTGC ACAGAGGGTG 
CAGGAGGAGC ATTGCAGCGC CGATTCTGCG TACACCTCGA CGTCGTCATC GGTGAGGCCA 
TTCGAGCCAC GGCAGCGGGA AGGCCTCACC AGCAGCGAGG CGGGTGGTCG CTTGTGGGTG 
TTTGCCAAGA CCATCCGAGA CAAGAAGGAC CTGCGCCAAC GGCAAGCTGC CCGGGACGCT 
TATCTGCAAG AGATGCACAA TGAAAATGCC ATTGATACCG ACGAAGAGGA GAAGCAGCAG 
GAGGAGCGCC CACCGACGCC CTCTTCGGGC GCAATTACGT GGCGGCGCTG CAGCAACGAG 
GTTAGCGGCG ACGATAGAGA GATGATGAGC CAGGAGGCGT TGCACCAGCT GTACCATCAA 
GAGCGCCGAT CCTTTGCCTC CTCGCTTGTA GGCGGCTTTG TCGATATTAT CAAGGCGAAG 
AAGCAGCGAG CCCATCGCCA AGAGGCGCGC GACGCCTACC TGGACCAGTG CGCGGCAGAG 
GATGCCGAGG CGCATAAGGA GGCGAGCGCG CGCGTGGTAA CTGACTTCAT GCGTAGCGCC 
GCCGCGCGCA GCACCTTGTG TAGCCTAGTC AAGACGATGA GCTCGTACAA CGACGCTCGT 
GCGATGCAGG AGGAGGATGA TAAGACGCCT GCGGCTGCGA CGACGGATGA TGGCAGCGGT 
GAGGGTGCGT ACGCAACCCC TGCCGAGTCG TGTGAGCACT CCCCTTTGTC TGCGGCTCTC 
AGAATCCAGC GCTTCTTCAG GGGCGTGCAG GCAAGGCGTC GAGTGCGAGT ACTGCAAGAG 
GCACACCGTG CCTACCTCGA GGAGGAGCTT GCAGTAGAGG AGCTACTCTA CTCAGCGGCG 
GTTGCCATTC AGTGCTTGTA CCGCGGCCAT CTGGCGCGTC GGCAGGCGGC CATGATCATT 
GTGCAGCGTG ATCTCTACCT GTTGTCACTC CAGGAAGAGG ATTGGTTAGG TGGGGATTCG 
AAGCGCGGAG AAGGGAGTGA TGCGTCCTTG CCAGCAGCTC TATCGTTTTT TGCTTCCTCA 
CCAAAGAGTG TCGCAGGCGG CGGAGCCAAG GCAGCGGCGA CTCGCCCCTT TACCCCACGC 
CGCTCTTCCT CGTCATCCTA TACTGGTCTG CGCGAAGTTG CCAGCACAGC CATTGTGACC 
GAGGTGAATG AGGCGGACGA GGCAGCGCTG GACCAGCTTT TGCATCGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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LMJF_32_3150 cDNA ORF clone, Leishmania major strain Friedlin

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Target ORF information:

ORF Insert Sequence:

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