ORF » Species Summary » Leishmania major strain Friedlin » LMJF_32_2290 cDNA ORF clone

LMJF_32_2290 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_32_2290 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_32_2290 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb06542 XM_001685500.1
Latest version!		 Leishmania major strain Friedlin putative sphingosine-1-phosphate phosphatase partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_32_2290 ( XM_001685500.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001685552.1 Leishmania major strain Friedlin putative sphingosine-1-phosphate phosphatase partial mRNA.
    Price & Availability↑
    CloneID OLb06542
    Clone ID Related Accession (Same CDS sequence) XM_001685500.1
    Accession Version XM_001685500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative sphingosine-1-phosphate phosphatase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007273). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTCGTCG TGACATCAAA GCCTGCCAGC CACTTTGTTG GCCCCTCCTC CGCTCAGGAC 
    TTCACGCCGG TGCCATCGCC ACCGTTGACG ACGCTGGCAT CGACCCGCAG GGATGCTGTT 
    CGAGAAGGCG TTGCTGTCAG CGCCACATGT ACTGACGATA CCACAAATGG CATTGGGAAT 
    GGTGGCATAG CCGGCGTTGC ACCATGCAAC GGGAACAACA GTGGCTGGCC TTACCCCCGC 
    GGCGGCGGCA ACCCAGCTGC CAATGGCACT GCTAGTGGTA AGTCGAAGCC AATCTCGCTG 
    GGTATCGTGC TGAAAGGCCC CAACAGAGAG GGGGAGAGCA ACCCGTCAGA CTTCGCGGAA 
    AACTCCGATA GCCTCGGCAC AGATGCACCC CAAGTCGGCG ACACCGTCGA GTCGGCAATC 
    AAGGTTCCCA TGACCGTCGT GACACCGGCG GCAGTTTCAA GCGATGTGGT GGCGTCGCCG 
    CTCACGGTGC ACGAAAATCT CGTGCAGTGG CGCCCCCCGC AGCGGGCGGT TGTGTTTCGC 
    GAGTGGTACA TCTCCTGCTT CTGTAACGAG CAGCAAGTGT GGTGGATCAA GAAGGCGCAG 
    CGCATAGGTG GCCCACTGCA GCCGCTGCTC GTGCCATACT TCCAGTTGTG GAGCTTCACT 
    GGCGAGGCTG AATTCTACAT TCTGTTCATT CCGACGACGG CTTGGCTTGG TGCGCCTCTC 
    AGCAGTGTGC AAATGGCGTC GATGCTATGG GTGGGGCAGT ACGTGACCAG CACCATGAAG 
    GACGCTTTCT GCTGCCCACG TCCGCCGTGT CCACCGCTTC AGCTGCACGG CAAGCGCGAC 
    GCTCACGACA ACGAGTACGG CTTCCCAAGC ACCCACTCCT GCCAAAGCGG CGTCTTCTCC 
    TTCTTGCTGT ACTGCCAACT GGTGCACGCC TTCCCTGATC ACGCCTTCCT GTGCTGGCTG 
    GCGGCCGTTT GCTACTTCGC GCACGTCAGT TTCTCGCGCA TCTACCTGGG CATGCACTGG 
    GTCGGTGACT TGATTGGTGG CGGTGTGGTG GCCTTCCTTA CTGTTCTCTC CCACGTCGCC 
    TTCCTCGATG GGTGGGAGGC CAGCATCCTG CAGCGTGCCG ACACGCCGTG GTGGGCGTAC 
    CTGCTTTTGT ACGTCACCGT GCACCTGCTC TCCATGGCCC ATGCCACCCC CCATGACCCG 
    TGCCCGTGCT ACATTGATAG CCTGCGCTTC ACCGGTGCGG TGATGGGCTC TGCGATCGGC 
    CTCTGGAGAT TCTACGCAAT CTACGGTACC CTGGCCGCAC GCCCGCAATC CGACCACATG 
    CTCGACGTGG TGTTCTCCTT CAGCTTTCTG ATGCAGTGGG TCGTGTGCAT GGCCGTTGTC 
    TTTGCCAGCA GGGAGCTGTC GTTGCTCATC GCCGCCGTTG TACTGAAGGC GGTCTTCAAG 
    TTCTTGTCGG GTGAGTGCGC GCCGCGGTTG CCCAAGTCGC TTCGGCAGCC CTACTTGGCC 
    ATGGCGAAGG TGGTCGGGCT GACCACGCTT GGCAACGAGC GAGGCTCGCG CTCCTGCATC 
    CCCGTCACCG CCAATGGTTC TCTCCCCATG CAAAGTGACC GCTTCAACGG CGGATTTGGC 
    CAAGACGAGA AAGCCGCCGC GGCCGCCGCT GACGGCAAGA ACGCGCTGAA TTCACGCAGC 
    AACACGGAAA CGGTGGAGGA GCTGGACGGG TACTTGAACA GTCAGCAAGT CTGGTCGCTC 
    CGCACTCACC GACACTGGTG GCTTTGGGAC GTGCACGGGC GCACCGTTTC CTATGCCGTC 
    ATGGGCTTCG TGGCCACATT TGTGTGCCAA GTGCTCTTGA GGGAAACTTT TGGGGTTGGT 
    CAGGAGCTTA CCGCGCTGGC AGCGCATCGT CCGTGGTCAC CACCACCGTC GCCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001685552.1
    CDS1..1920
    Translation

    Target ORF information:

    RefSeq Version XM_001685500.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative sphingosine-1-phosphate phosphatase partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001685500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTCGTCG TGACATCAAA GCCTGCCAGC CACTTTGTTG GCCCCTCCTC CGCTCAGGAC 
    TTCACGCCGG TGCCATCGCC ACCGTTGACG ACGCTGGCAT CGACCCGCAG GGATGCTGTT 
    CGAGAAGGCG TTGCTGTCAG CGCCACATGT ACTGACGATA CCACAAATGG CATTGGGAAT 
    GGTGGCATAG CCGGCGTTGC ACCATGCAAC GGGAACAACA GTGGCTGGCC TTACCCCCGC 
    GGCGGCGGCA ACCCAGCTGC CAATGGCACT GCTAGTGGTA AGTCGAAGCC AATCTCGCTG 
    GGTATCGTGC TGAAAGGCCC CAACAGAGAG GGGGAGAGCA ACCCGTCAGA CTTCGCGGAA 
    AACTCCGATA GCCTCGGCAC AGATGCACCC CAAGTCGGCG ACACCGTCGA GTCGGCAATC 
    AAGGTTCCCA TGACCGTCGT GACACCGGCG GCAGTTTCAA GCGATGTGGT GGCGTCGCCG 
    CTCACGGTGC ACGAAAATCT CGTGCAGTGG CGCCCCCCGC AGCGGGCGGT TGTGTTTCGC 
    GAGTGGTACA TCTCCTGCTT CTGTAACGAG CAGCAAGTGT GGTGGATCAA GAAGGCGCAG 
    CGCATAGGTG GCCCACTGCA GCCGCTGCTC GTGCCATACT TCCAGTTGTG GAGCTTCACT 
    GGCGAGGCTG AATTCTACAT TCTGTTCATT CCGACGACGG CTTGGCTTGG TGCGCCTCTC 
    AGCAGTGTGC AAATGGCGTC GATGCTATGG GTGGGGCAGT ACGTGACCAG CACCATGAAG 
    GACGCTTTCT GCTGCCCACG TCCGCCGTGT CCACCGCTTC AGCTGCACGG CAAGCGCGAC 
    GCTCACGACA ACGAGTACGG CTTCCCAAGC ACCCACTCCT GCCAAAGCGG CGTCTTCTCC 
    TTCTTGCTGT ACTGCCAACT GGTGCACGCC TTCCCTGATC ACGCCTTCCT GTGCTGGCTG 
    GCGGCCGTTT GCTACTTCGC GCACGTCAGT TTCTCGCGCA TCTACCTGGG CATGCACTGG 
    GTCGGTGACT TGATTGGTGG CGGTGTGGTG GCCTTCCTTA CTGTTCTCTC CCACGTCGCC 
    TTCCTCGATG GGTGGGAGGC CAGCATCCTG CAGCGTGCCG ACACGCCGTG GTGGGCGTAC 
    CTGCTTTTGT ACGTCACCGT GCACCTGCTC TCCATGGCCC ATGCCACCCC CCATGACCCG 
    TGCCCGTGCT ACATTGATAG CCTGCGCTTC ACCGGTGCGG TGATGGGCTC TGCGATCGGC 
    CTCTGGAGAT TCTACGCAAT CTACGGTACC CTGGCCGCAC GCCCGCAATC CGACCACATG 
    CTCGACGTGG TGTTCTCCTT CAGCTTTCTG ATGCAGTGGG TCGTGTGCAT GGCCGTTGTC 
    TTTGCCAGCA GGGAGCTGTC GTTGCTCATC GCCGCCGTTG TACTGAAGGC GGTCTTCAAG 
    TTCTTGTCGG GTGAGTGCGC GCCGCGGTTG CCCAAGTCGC TTCGGCAGCC CTACTTGGCC 
    ATGGCGAAGG TGGTCGGGCT GACCACGCTT GGCAACGAGC GAGGCTCGCG CTCCTGCATC 
    CCCGTCACCG CCAATGGTTC TCTCCCCATG CAAAGTGACC GCTTCAACGG CGGATTTGGC 
    CAAGACGAGA AAGCCGCCGC GGCCGCCGCT GACGGCAAGA ACGCGCTGAA TTCACGCAGC 
    AACACGGAAA CGGTGGAGGA GCTGGACGGG TACTTGAACA GTCAGCAAGT CTGGTCGCTC 
    CGCACTCACC GACACTGGTG GCTTTGGGAC GTGCACGGGC GCACCGTTTC CTATGCCGTC 
    ATGGGCTTCG TGGCCACATT TGTGTGCCAA GTGCTCTTGA GGGAAACTTT TGGGGTTGGT 
    CAGGAGCTTA CCGCGCTGGC AGCGCATCGT CCGTGGTCAC CACCACCGTC GCCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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