ORF » Species Summary » Leishmania major strain Friedlin » ILERS cDNA ORF clone

ILERS cDNA ORF clone, Leishmania major strain Friedlin

The following ILERS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ILERS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb06156 XM_001687097.1
Latest version!		 Leishmania major strain Friedlin putative isoleucyl-tRNA synthetase (ILERS), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ILERS ( XM_001687097.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001687149.1 Leishmania major strain Friedlin putative isoleucyl-tRNA synthetase (ILERS), partial mRNA.
    Price & Availability↑
    CloneID OLb06156
    Clone ID Related Accession (Same CDS sequence) XM_001687097.1
    Accession Version XM_001687097.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative isoleucyl-tRNA synthetase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007287). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGGGCG AGGGTCAGCA GCAGCAGCAA GACCCCCCGG CGGGGCAGCA GGGCGTACCA 
    GTGAAGAACT ATCCCGATGT GCTCGACTTC GCGAAGATGG AGGAGGAAGT GCTGGCGATG 
    TGGCACGAGA AGGATTGCTT TCGCACTTCC ATGCAGCTCT CCGAGGGTCG CCAGCCGTTC 
    AGCTTCTACG ACGGCCCACC GTTCGCGACT GGTCTGCCGC ACTACGGCCA CATCTTGGCA 
    GGCACCATCA AGGACATGGT CACTCGCTTT GCCTACCAGA CGAACCACCA CGTCATCCGT 
    CGCTTCGGCT GGGACTGCCA CGGTCTGCCG GTGGAGTACG AGATCGACAA GATGCTCGGC 
    ATCAAGACGT CCCACGACGT GGCGAAGCTC GGCATTGACA AGTACAACGA CGAGTGCAAG 
    AAGATCGTCA CCCGCTACGT TGGCGAGTGG AGGAAGACCG TCGAGCGCGT TGGCCGCTGG 
    ATCGACTTTG ACGACGACTA CAAGACGATG CACCTCACGT ACATGGAGAG TGTCTGGTGG 
    GTCTTCTCGC AGCTGTGGGA GAAGAAGATG GTGTACCGCG GCTTTCGCGT GATGCCGTAC 
    TCGACCGCCT GCACGACGCC GCTGAGCAAC TTTGAGGCGA ACTCCAACTA CAAGGAGGTC 
    AGCGACCTCG CCGTGACGGT CGCCTTCCAG GCCCGCGCCG ACCCCAACAC ATACTTCCTG 
    GCCTGGACCA CCACGCCGTG GACGTTGCCG AGCAACCTCA GTTTGTGCGT GCACCCCGAG 
    CTGGACTACG TGAAGGTGCT GGACACTAAG TCGAAGCGAC ACTACTGGCT CGCGGAGGCC 
    CGCCTGGGGG AGGTGTACCC GAAGAGGGAC AGCCAGAAGG CCTCCAAGTC AAAGGACGGC 
    GACAACGCCG CGGAGGCCGG TGCCGCGCCG TACACGGTCG TCGAGAAGGT CAAGGGCGCG 
    CAGCTAGTGG GCGAGAAGTA CGTGCCACTT TTCCCGTACT TTGAGTCGTC CATGAGCGCC 
    ACGGCCTTCC GCGTCATCTC TGGTACCTAC GTGACGACAG ACGCTGGTAC AGGTATCGTG 
    CACCAGGCCC CTGCCTTCGG TGAGGATGAT TACCAGGCGT GCCTCGACGC CGGCATCTTC 
    GAGAAGGGTG GCAAGTTCGT CTGTCCGGTC GACGAGAACG GCATGTTCAC GAGCGAGGTG 
    ACGGATTTCG CCGGTAAGTA CGTCAAGGAG GCGGATCCGG AGATTATCAA AGCGCTCGAG 
    ACGAAAGGGC ACCTCTTTCA CAAGGCTTCG ATTGTGCACA GCTACCCCTT CTGCTGGCGC 
    AGCGACACAC CCCTCATCTA CAAGGCTGTC GAGTCGTGGT TTGTCAACGT CGAGGCCTTC 
    CGCGACCGCC TCATCGAATG CAATGACAAG ACATACTGGG TGCCTGATTT TGTCAAGACC 
    CGCCGCTTCT CCAACTGGCT CGCCGAGGCG CGCGACTGGA ACGTGTCACG CAACCGCTAC 
    TGGGGTACGC CGATGCCGGT GTGGCACAGC GAGGACTGGG AAGAGGTTGT GTGCGTCAGC 
    AACGCCAAGC AGCTCGAGGA GCTCTCCGGC GTCACCGGCA TCACAGACAT CCACCGCCAG 
    TTCGTTGACA ATATCACGAT CCCAAGTAAG CGGCCTGGTA TGCCGCCGCT GAAGCGTGTG 
    CCGATGGTGT TCGACTGCTG GTTCGAGTCC GGCTCGATGC CGTACGCCCA GGAACACTTC 
    CCATTCGAGA ACCAGGACAA GTTCAAGAGC CTGTTTCCGG CCGACTTTGT GTCGGAGGGC 
    TTGGACCAGA CGCGCGGCTG GTTCTACACC CTTCTCGTGC TCGGTGTTGC CCTCTTCGAC 
    GTCTCCCCTT TCCGCAACGT CGCCGTCAGC GGGCTCGTGC TCGCCGAGGA CGGTAAGAAG 
    ATGAGCAAGC GCCTCAAGAA CTACCCTGAG CCGAAGGTAG TGATCGACAC GTACGGCGCG 
    GATGCCTTGC GCATGTACAT GATTAGCTCC CCGGTTGTGC GCGCCGAGCC GCTGCGCTTC 
    CGCGAGGCAG GTGTGAAGGG CGTGGTCAAG GACATCCTCC TGCCTCTGTT CAACGCCGCG 
    AAGTTCTTCA TCTCGAACAC GAACTACTGC ATTGCGGCCG GTGGTCAGGT GTCGCTGCAG 
    GTTCGCAGCA CAAACGAGAT GGACCGCTGG ATTCTCGCCT CGTGCCAGAG TCTGCTGCGC 
    TACGTGAAGG CGGAGATGCG GCTGTACCAC CTGTACAACG TCGTTCCCGG CATTCTGCGT 
    TTCGTCGGTG ACCTGTCCAA CTGGTACGTG CGCATGAACC GCCGCCGCAT GAAGAACGCC 
    ACGGACAGCG AGGACCGCTC GCAGGCGCTG TCGACGATGC TGTACCTCCT CTTCTCGGTG 
    TCTCGCATTG TGGGCCACAT TGCCCCCTTT GTGGCAGAAA TGCTTTACCA GCAGATCAAA 
    CCCCTCCTCC CGGCGAATGA GCGCGTAGAC TCCGTGCACT ACCTCATGAT CCCCGACGAG 
    GACGCCTCCT TGGACGACCC GGAGCTGGAG CGTGCTATGT CCCGCATGAT GAACATTGTG 
    GACTTGGTGC GTGTGCTGCG CGATCAGATG GTCATTCCAA TCAAGCGGCC GGTGCGTCAG 
    GTCGTCATTG TTCACCCGGA CCAGCAGTAC ATCGACGACG TGCGCAAGGT GGTGGGCTAC 
    ATCAAGGATG AGGTGAACGC GTTCGAGATT GTGATGTCGA GTGGCGAGAA TTACGTGGAG 
    ACGAAGCTGG ACGCGAACTT CGAGGTGCTT GGCAAGAAGT ACCGCAAGGA GATGCCCGCG 
    ATCCGCAAGG GTATCCAGGC CATGACGCAG GAGGAGGTGA CCAAGTTCCT GAACGACAAG 
    AAGGGCGTCG TCGTCGGCAA GGAGCTGACC ATCGAGGACG TGAAGGTGCT GCGCCAGGTA 
    AAGGAGGGCA TTACCGACTT CCAGAGCAAC ACTGACAACA ACGTCGTTGT GCTGGTTGAC 
    AAGCGCCAGG ATCAGGAGCT GATCGACTCG TGGCGCGCGC GTGAGTTCGT GAACCGCGTA 
    CAGCAGCTGC GTAAGAAGGC GAAGCTGGTG GTGACGGACG AAGTGGATGT GTACTTTGAG 
    GCGGAGGAGG CGGATCTGAC GGCGTCCATC CTCCACTCTA AGGACCAGAT TAATCAGACA 
    TTGAGGGGCG TGTGGACGAC AATGGACCAG AAGCCTAGCG ACGCCGAGCT CGTTGCGGAG 
    GAGGACAACA GCATCTCGGG GGTGGCGGTG CGCCTCGTCC TCACTAAGCC CAAGACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001687149.1
    CDS1..3300
    Translation

    Target ORF information:

    RefSeq Version XM_001687097.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative isoleucyl-tRNA synthetase (ILERS), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001687097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCGGGCG AGGGTCAGCA GCAGCAGCAA GACCCCCCGG CGGGGCAGCA GGGCGTACCA 
    GTGAAGAACT ATCCCGATGT GCTCGACTTC GCGAAGATGG AGGAGGAAGT GCTGGCGATG 
    TGGCACGAGA AGGATTGCTT TCGCACTTCC ATGCAGCTCT CCGAGGGTCG CCAGCCGTTC 
    AGCTTCTACG ACGGCCCACC GTTCGCGACT GGTCTGCCGC ACTACGGCCA CATCTTGGCA 
    GGCACCATCA AGGACATGGT CACTCGCTTT GCCTACCAGA CGAACCACCA CGTCATCCGT 
    CGCTTCGGCT GGGACTGCCA CGGTCTGCCG GTGGAGTACG AGATCGACAA GATGCTCGGC 
    ATCAAGACGT CCCACGACGT GGCGAAGCTC GGCATTGACA AGTACAACGA CGAGTGCAAG 
    AAGATCGTCA CCCGCTACGT TGGCGAGTGG AGGAAGACCG TCGAGCGCGT TGGCCGCTGG 
    ATCGACTTTG ACGACGACTA CAAGACGATG CACCTCACGT ACATGGAGAG TGTCTGGTGG 
    GTCTTCTCGC AGCTGTGGGA GAAGAAGATG GTGTACCGCG GCTTTCGCGT GATGCCGTAC 
    TCGACCGCCT GCACGACGCC GCTGAGCAAC TTTGAGGCGA ACTCCAACTA CAAGGAGGTC 
    AGCGACCTCG CCGTGACGGT CGCCTTCCAG GCCCGCGCCG ACCCCAACAC ATACTTCCTG 
    GCCTGGACCA CCACGCCGTG GACGTTGCCG AGCAACCTCA GTTTGTGCGT GCACCCCGAG 
    CTGGACTACG TGAAGGTGCT GGACACTAAG TCGAAGCGAC ACTACTGGCT CGCGGAGGCC 
    CGCCTGGGGG AGGTGTACCC GAAGAGGGAC AGCCAGAAGG CCTCCAAGTC AAAGGACGGC 
    GACAACGCCG CGGAGGCCGG TGCCGCGCCG TACACGGTCG TCGAGAAGGT CAAGGGCGCG 
    CAGCTAGTGG GCGAGAAGTA CGTGCCACTT TTCCCGTACT TTGAGTCGTC CATGAGCGCC 
    ACGGCCTTCC GCGTCATCTC TGGTACCTAC GTGACGACAG ACGCTGGTAC AGGTATCGTG 
    CACCAGGCCC CTGCCTTCGG TGAGGATGAT TACCAGGCGT GCCTCGACGC CGGCATCTTC 
    GAGAAGGGTG GCAAGTTCGT CTGTCCGGTC GACGAGAACG GCATGTTCAC GAGCGAGGTG 
    ACGGATTTCG CCGGTAAGTA CGTCAAGGAG GCGGATCCGG AGATTATCAA AGCGCTCGAG 
    ACGAAAGGGC ACCTCTTTCA CAAGGCTTCG ATTGTGCACA GCTACCCCTT CTGCTGGCGC 
    AGCGACACAC CCCTCATCTA CAAGGCTGTC GAGTCGTGGT TTGTCAACGT CGAGGCCTTC 
    CGCGACCGCC TCATCGAATG CAATGACAAG ACATACTGGG TGCCTGATTT TGTCAAGACC 
    CGCCGCTTCT CCAACTGGCT CGCCGAGGCG CGCGACTGGA ACGTGTCACG CAACCGCTAC 
    TGGGGTACGC CGATGCCGGT GTGGCACAGC GAGGACTGGG AAGAGGTTGT GTGCGTCAGC 
    AACGCCAAGC AGCTCGAGGA GCTCTCCGGC GTCACCGGCA TCACAGACAT CCACCGCCAG 
    TTCGTTGACA ATATCACGAT CCCAAGTAAG CGGCCTGGTA TGCCGCCGCT GAAGCGTGTG 
    CCGATGGTGT TCGACTGCTG GTTCGAGTCC GGCTCGATGC CGTACGCCCA GGAACACTTC 
    CCATTCGAGA ACCAGGACAA GTTCAAGAGC CTGTTTCCGG CCGACTTTGT GTCGGAGGGC 
    TTGGACCAGA CGCGCGGCTG GTTCTACACC CTTCTCGTGC TCGGTGTTGC CCTCTTCGAC 
    GTCTCCCCTT TCCGCAACGT CGCCGTCAGC GGGCTCGTGC TCGCCGAGGA CGGTAAGAAG 
    ATGAGCAAGC GCCTCAAGAA CTACCCTGAG CCGAAGGTAG TGATCGACAC GTACGGCGCG 
    GATGCCTTGC GCATGTACAT GATTAGCTCC CCGGTTGTGC GCGCCGAGCC GCTGCGCTTC 
    CGCGAGGCAG GTGTGAAGGG CGTGGTCAAG GACATCCTCC TGCCTCTGTT CAACGCCGCG 
    AAGTTCTTCA TCTCGAACAC GAACTACTGC ATTGCGGCCG GTGGTCAGGT GTCGCTGCAG 
    GTTCGCAGCA CAAACGAGAT GGACCGCTGG ATTCTCGCCT CGTGCCAGAG TCTGCTGCGC 
    TACGTGAAGG CGGAGATGCG GCTGTACCAC CTGTACAACG TCGTTCCCGG CATTCTGCGT 
    TTCGTCGGTG ACCTGTCCAA CTGGTACGTG CGCATGAACC GCCGCCGCAT GAAGAACGCC 
    ACGGACAGCG AGGACCGCTC GCAGGCGCTG TCGACGATGC TGTACCTCCT CTTCTCGGTG 
    TCTCGCATTG TGGGCCACAT TGCCCCCTTT GTGGCAGAAA TGCTTTACCA GCAGATCAAA 
    CCCCTCCTCC CGGCGAATGA GCGCGTAGAC TCCGTGCACT ACCTCATGAT CCCCGACGAG 
    GACGCCTCCT TGGACGACCC GGAGCTGGAG CGTGCTATGT CCCGCATGAT GAACATTGTG 
    GACTTGGTGC GTGTGCTGCG CGATCAGATG GTCATTCCAA TCAAGCGGCC GGTGCGTCAG 
    GTCGTCATTG TTCACCCGGA CCAGCAGTAC ATCGACGACG TGCGCAAGGT GGTGGGCTAC 
    ATCAAGGATG AGGTGAACGC GTTCGAGATT GTGATGTCGA GTGGCGAGAA TTACGTGGAG 
    ACGAAGCTGG ACGCGAACTT CGAGGTGCTT GGCAAGAAGT ACCGCAAGGA GATGCCCGCG 
    ATCCGCAAGG GTATCCAGGC CATGACGCAG GAGGAGGTGA CCAAGTTCCT GAACGACAAG 
    AAGGGCGTCG TCGTCGGCAA GGAGCTGACC ATCGAGGACG TGAAGGTGCT GCGCCAGGTA 
    AAGGAGGGCA TTACCGACTT CCAGAGCAAC ACTGACAACA ACGTCGTTGT GCTGGTTGAC 
    AAGCGCCAGG ATCAGGAGCT GATCGACTCG TGGCGCGCGC GTGAGTTCGT GAACCGCGTA 
    CAGCAGCTGC GTAAGAAGGC GAAGCTGGTG GTGACGGACG AAGTGGATGT GTACTTTGAG 
    GCGGAGGAGG CGGATCTGAC GGCGTCCATC CTCCACTCTA AGGACCAGAT TAATCAGACA 
    TTGAGGGGCG TGTGGACGAC AATGGACCAG AAGCCTAGCG ACGCCGAGCT CGTTGCGGAG 
    GAGGACAACA GCATCTCGGG GGTGGCGGTG CGCCTCGTCC TCACTAAGCC CAAGACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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