ORF » Species Summary » Leishmania major strain Friedlin » MEFG cDNA ORF clone

MEFG cDNA ORF clone, Leishmania major strain Friedlin

The following MEFG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEFG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb05640 XM_001686577.1
Latest version!		 Leishmania major strain Friedlin putative Mitochondrial elongation factor G (MEFG), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MEFG ( XM_001686577.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686629.1 Leishmania major strain Friedlin putative Mitochondrial elongation factor G (MEFG), partial mRNA.
    Price & Availability↑
    CloneID OLb05640
    Clone ID Related Accession (Same CDS sequence) XM_001686577.1
    Accession Version XM_001686577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative Mitochondrial elongation factor G
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007287). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCGCAGAA GCGGCCTGGC CTACATGCTG CGCAGCAGCG TCCCGCTGCT CGCAACACCC 
    GCTTTCCTCG CGAATGTGAA GCACATGCGC AACATTGGTA TCAGTGCCCA CATTGATAGC 
    GGCAAAACGA CGCTGAGTGA GCGCATTCTC TTCTACTCCG GTCGCATCGG AAAGATCCAC 
    GAGGTGAAGG GCGGCACCGA GGTCGGCGCT ACAATGGACT CCATGGAGCT TGAGAAAGAG 
    CGTGGTATCA CTATTCGCTC AGCCGCAACG CAGTGCCGCT GGAAGAACAG TACCATCAAT 
    ATAATCGACA CACCCGGCCA CGTCGACTTC ACCATCGAAG TGGAGCGCGC CCTTCGCGTG 
    CTGGACGGTG CTATCTTGCT CATGTGCGCC GTTGGCGGTG TGCAGAGTCA GACGCTGACG 
    GTGGATCGCC AGATGAAGCG GTACGGGGTA CCGCGCATCT GCTTCATAAA CAAGCTCGAT 
    CGCGACAACG CGAACCCACA GCGGGCAGTA AAGCAAGCGC AGGAGCGTCT GGGTATCAAC 
    GCCGTCTTCA TCCAGCTGAA CATGGGTACT GCACAGGACT TCGAAGGCGT CGTCGACCTC 
    ATCGAGGAGA AGGCCGTCTA CTTTGATGGC CCTTTCGGTG AGGCCATCCG CTACGAACCG 
    GTGCCGAGCT ACATCAAGGA GGACGTGGTT GCCGCGCGCA AGGAGCTCGT GAGCCGCCTA 
    GCAGAGTGCG ACGAAGAGAT GGAGTTCATC TTTCTTAATG ACCAGGAGCC GACAGTCGAG 
    CAGATCCACT CTGCCATTCG CCGCGCTACC ATCGCCAACA AGTTTGTGCC CGTGATGGTC 
    GGCTCGGCGT ACCGGAACAA GGGCGTGCAG CTGCTCCTGG ACGCGGTAGA GCGCTACCTG 
    CCGTCGCCAG TGGAGCGCCA CAACTCCGGC TATCAGGTGA GGCGTGTGAA AGACGAGGAC 
    GGCAACGTGT CGAACGTGAA GGAAGGTGAG GTGGCGTTGA TGACGGATGA CGAAAAGCCG 
    CTGGTGGCCC TCATTTTCAA GATCGAGGAA ACAAAGAAGT CGGGGCTGTC CAACTACGTG 
    CGGGTGTACC AGGGGAAGAT GCGCAAGGAG CACCTGATGA ATATCCGCAC CGGCAAGAAC 
    TTCTTGCCGC CGAAGCTTGT GCGCATGCAC GCGGACAGCG CCGAGGTTGT GGACGAGGTG 
    CGCGCTGGCG ACATCTGTGC CATTCAGGGT GAGGTGGATG CGTCCTCGGG TGATACCCTC 
    ATGAAGTCCG GACCGCAGTC TGGTTCGCAG CTCTTCTCGT GCGAGGACAT GTACGTTCCG 
    CCGCGGGTCA TCTCTGCCTC ACTCAAGACG AAGGATGACA AGGAGCAGTC CAGGGTGCGG 
    GAGCGCATGC TCGCCTTCAT GCGAGAAGAC CCCACCTTTG TGTATTACCG CAACTCGGAG 
    ACGAACGAGG ACATTGTGGA GGGCATGGGT GAGTTGCACC TCGATATTTA CGTGGAGCGG 
    CTGAAGCGGG AGTACGGACT GCACGTGGAG CTCGGCAAAC CAACGGTCAA CTACCGCGAG 
    ATTATCACGG AGCGGCAGGA GTTCGACTTT GTCTTCAAGC GGCAAAGCGG CGGTGCGGGT 
    CAGTGGGCGC ACCTGAAGGG CTATGCCGAG CCGCTGCCGA TCGATATGTC GGTCGAGAAG 
    GGCGTCAAGA ACAAAGCGAC GACACGATGC TCGAACGGTG ACATCCGCGA GTCGCTGCAG 
    AAGACGGTGG TGAAGCACCT GGAGCGCAAA ATTTTTGTAA AGGGCGAGCT GATGCACGCA 
    CCTGTCTGGG GCGTGCACTT TCACTTGAAT GGTGGCGCCA TGCACGAGGT AGACTCGAAC 
    GATCAGGCCT TCAAGAACGC AACCCAGGAG CTGTGGGAAA CCCTGCTACC GAAGCTGAAG 
    CCGACGCTTG TGGAGCCGTT CATGGATGTG GAGATGACAG TGCCTGCGGC GAACATGACT 
    GATGTGGCGA CCGAGTTCTC GAAGCGAGAG GGCGTGGTGA CCGAGACCGC TGTAGATGGC 
    CCTGATGCAG TGATCCGCGG GGAGACAGCC CTGGACACCA TGTTCGGCTT TATTTCCGAC 
    CTGCGACGAC TCACAAAGGG CCAGGGCGAC TTCAGCATGC AGTTTAAGGA ATACCGGCCG 
    ATGCAGCAGT ACAAGGCCCA AATGCGAATG GATGAACGCA ACAACGATCT TGGGCGCAAG 
    CCATTCAAGC TTTCGGACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001686629.1
    CDS1..2241
    Translation

    Target ORF information:

    RefSeq Version XM_001686577.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative Mitochondrial elongation factor G (MEFG), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001686577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCGCAGAA GCGGCCTGGC CTACATGCTG CGCAGCAGCG TCCCGCTGCT CGCAACACCC 
    GCTTTCCTCG CGAATGTGAA GCACATGCGC AACATTGGTA TCAGTGCCCA CATTGATAGC 
    GGCAAAACGA CGCTGAGTGA GCGCATTCTC TTCTACTCCG GTCGCATCGG AAAGATCCAC 
    GAGGTGAAGG GCGGCACCGA GGTCGGCGCT ACAATGGACT CCATGGAGCT TGAGAAAGAG 
    CGTGGTATCA CTATTCGCTC AGCCGCAACG CAGTGCCGCT GGAAGAACAG TACCATCAAT 
    ATAATCGACA CACCCGGCCA CGTCGACTTC ACCATCGAAG TGGAGCGCGC CCTTCGCGTG 
    CTGGACGGTG CTATCTTGCT CATGTGCGCC GTTGGCGGTG TGCAGAGTCA GACGCTGACG 
    GTGGATCGCC AGATGAAGCG GTACGGGGTA CCGCGCATCT GCTTCATAAA CAAGCTCGAT 
    CGCGACAACG CGAACCCACA GCGGGCAGTA AAGCAAGCGC AGGAGCGTCT GGGTATCAAC 
    GCCGTCTTCA TCCAGCTGAA CATGGGTACT GCACAGGACT TCGAAGGCGT CGTCGACCTC 
    ATCGAGGAGA AGGCCGTCTA CTTTGATGGC CCTTTCGGTG AGGCCATCCG CTACGAACCG 
    GTGCCGAGCT ACATCAAGGA GGACGTGGTT GCCGCGCGCA AGGAGCTCGT GAGCCGCCTA 
    GCAGAGTGCG ACGAAGAGAT GGAGTTCATC TTTCTTAATG ACCAGGAGCC GACAGTCGAG 
    CAGATCCACT CTGCCATTCG CCGCGCTACC ATCGCCAACA AGTTTGTGCC CGTGATGGTC 
    GGCTCGGCGT ACCGGAACAA GGGCGTGCAG CTGCTCCTGG ACGCGGTAGA GCGCTACCTG 
    CCGTCGCCAG TGGAGCGCCA CAACTCCGGC TATCAGGTGA GGCGTGTGAA AGACGAGGAC 
    GGCAACGTGT CGAACGTGAA GGAAGGTGAG GTGGCGTTGA TGACGGATGA CGAAAAGCCG 
    CTGGTGGCCC TCATTTTCAA GATCGAGGAA ACAAAGAAGT CGGGGCTGTC CAACTACGTG 
    CGGGTGTACC AGGGGAAGAT GCGCAAGGAG CACCTGATGA ATATCCGCAC CGGCAAGAAC 
    TTCTTGCCGC CGAAGCTTGT GCGCATGCAC GCGGACAGCG CCGAGGTTGT GGACGAGGTG 
    CGCGCTGGCG ACATCTGTGC CATTCAGGGT GAGGTGGATG CGTCCTCGGG TGATACCCTC 
    ATGAAGTCCG GACCGCAGTC TGGTTCGCAG CTCTTCTCGT GCGAGGACAT GTACGTTCCG 
    CCGCGGGTCA TCTCTGCCTC ACTCAAGACG AAGGATGACA AGGAGCAGTC CAGGGTGCGG 
    GAGCGCATGC TCGCCTTCAT GCGAGAAGAC CCCACCTTTG TGTATTACCG CAACTCGGAG 
    ACGAACGAGG ACATTGTGGA GGGCATGGGT GAGTTGCACC TCGATATTTA CGTGGAGCGG 
    CTGAAGCGGG AGTACGGACT GCACGTGGAG CTCGGCAAAC CAACGGTCAA CTACCGCGAG 
    ATTATCACGG AGCGGCAGGA GTTCGACTTT GTCTTCAAGC GGCAAAGCGG CGGTGCGGGT 
    CAGTGGGCGC ACCTGAAGGG CTATGCCGAG CCGCTGCCGA TCGATATGTC GGTCGAGAAG 
    GGCGTCAAGA ACAAAGCGAC GACACGATGC TCGAACGGTG ACATCCGCGA GTCGCTGCAG 
    AAGACGGTGG TGAAGCACCT GGAGCGCAAA ATTTTTGTAA AGGGCGAGCT GATGCACGCA 
    CCTGTCTGGG GCGTGCACTT TCACTTGAAT GGTGGCGCCA TGCACGAGGT AGACTCGAAC 
    GATCAGGCCT TCAAGAACGC AACCCAGGAG CTGTGGGAAA CCCTGCTACC GAAGCTGAAG 
    CCGACGCTTG TGGAGCCGTT CATGGATGTG GAGATGACAG TGCCTGCGGC GAACATGACT 
    GATGTGGCGA CCGAGTTCTC GAAGCGAGAG GGCGTGGTGA CCGAGACCGC TGTAGATGGC 
    CCTGATGCAG TGATCCGCGG GGAGACAGCC CTGGACACCA TGTTCGGCTT TATTTCCGAC 
    CTGCGACGAC TCACAAAGGG CCAGGGCGAC TTCAGCATGC AGTTTAAGGA ATACCGGCCG 
    ATGCAGCAGT ACAAGGCCCA AATGCGAATG GATGAACGCA ACAACGATCT TGGGCGCAAG 
    CCATTCAAGC TTTCGGACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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