ORF » Species Summary » Leishmania major strain Friedlin » PUF1 cDNA ORF clone

PUF1 cDNA ORF clone, Leishmania major strain Friedlin

The following PUF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb05591 XM_001686522.1
Latest version!		 Leishmania major strain Friedlin putative PUF1 (PUF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PUF1 ( XM_001686522.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686574.1 Leishmania major strain Friedlin putative PUF1 (PUF1), partial mRNA.
    Price & Availability↑
    CloneID OLb05591
    Clone ID Related Accession (Same CDS sequence) XM_001686522.1
    Accession Version XM_001686522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative PUF1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007287). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCGTCAG AGGAGAAGTT AACAGCCCTC TACGCGCGCC GCCAGGAGAT TCTCAAGGAC 
    CTTTCTGAGG TGGATACGAG CATCAAGTTC CTCGAGAGTG AGATCCAAAC CAAGGCAACA 
    GAGGCGGCGA AGCGCACCAT CGATGAAAAC AAGTCAGGCA ACGCGGACCT GGATCGAGTG 
    AACGAGGTGA TCGCCAGCTG CGTGCGGGAT GCCACGGGGC TTCGCGACGC ACTCAAGCTG 
    GTTGAGGCGT GCCGCTACGT ACAGGAATTT GGCAAGGAGG ATGACGCGGC GCGGCAAAAG 
    ATCCCCTTCC GCAACGTGTT CGCGCAGGAG TGCATTCGCC ATGCGCTGGA CCTGTCCAAT 
    GACGCCAACG GAAGCGAACT GATGCAGCAC CTGGTGCCGC TGCTGCGCTC GGGAACCAGG 
    CCGGTTACCA TGGGCGACAT TTTCTACGAC CCCACAACGA ACGAGCACGA CCTGTCAGAA 
    GTGCTGCTGC TGATTAGGGA GCTTTCCAGC GATATTGTAA CGGTGGCGTG CAACACGAAT 
    GGCGCGCGTG TCTCCCAGCG CATCATCGAT GTGCTTTGCA CACATGAGGA GTTTGATGTG 
    TACACGAGTG TGCTTGAGCC ATCGATTGTC GACGTTGCGA AGGACATTAA TGGCAACCAC 
    TCCCTGTCAA AGCTGATCAC GTCTGCCCGC TTTTGCCAGC TGGGCGACTC TGACAAGAGT 
    GCCTCCGGCG CTGCTGCGAT CTACGAGCGC ATTTTCCAGA AGATAGCGGA CAACTGCATC 
    GACATTTGCA AAAACCGTCA GGGGTGCTGT ATCATTCAAA AGTGCCTGCA ACACGCCCCC 
    CAGCCGTATC ACACTACCAT CATCAACACG GTACTCAACA ACTCGCTGAA GCTTGTGCAG 
    GATCCCTTTG GCAACTATGT TGTTCAGTTC ATTCTCGACA AACAGCAAGA CATCAACGGC 
    GCAAAGAAGG AGGACGCTGA CGACGACGCC ATGTCCACGG CACCGAACTA CACCAATCAA 
    ATTATTAGGC AGATGCTCCA TCACGTGGCC GAGCTCTCGT GCAACAAGTT TAGCAGCAAT 
    GTGATTGAGA AGTGCCTGAA GACGTCGTCG CCGGATGTGC GACAGCTGTT GGTCGACGAG 
    CTCACGGCGC CGCATGTCTT GCCTAAGCTG CTGACAGATA GCTTCGCGAA TTACGTTATT 
    CAGACCGCCA TTTCAACCGC CTCCGACGAT GGCCAACTGA CGCAGCTGCG CGACGCTATC 
    ATACCTCTGC AGAGTCTCCT GAAAAACTCC CCGTATGGCG TGAAGATCGA GTCGAAGCTG 
    TCCCGGCGCC ACCGTGAAGC GGCGCGGCGT CTGCTGAAGA AAAAGGAGGC CGCCACGACC 
    GTCACACAGG CCCCACCCGC ACAGCAAGAG CCGCTTCTTC CGCCATACAT GCCCCCACAA 
    GGAATGTCGG CCATTCCAAT GATGGCGCCA GACGCTTCCA TGGGTCAGCA AGTGTCCTTC 
    ACCAACCTGA ATACGGACAT GGCCTCCTTC TTGCAGCCAC AGCGGATGAT AGGCATGCCT 
    TTTGTGTTGC AAGGACAACA GATGATCAGC ATCCCCAATG CACCACCGCC GTCTTTCACC 
    ACGGGTATGA TCAACGGTAG CGGCCTGCCT GAGTACCGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001686574.1
    CDS1..1662
    Translation

    Target ORF information:

    RefSeq Version XM_001686522.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative PUF1 (PUF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001686522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCGTCAG AGGAGAAGTT AACAGCCCTC TACGCGCGCC GCCAGGAGAT TCTCAAGGAC 
    CTTTCTGAGG TGGATACGAG CATCAAGTTC CTCGAGAGTG AGATCCAAAC CAAGGCAACA 
    GAGGCGGCGA AGCGCACCAT CGATGAAAAC AAGTCAGGCA ACGCGGACCT GGATCGAGTG 
    AACGAGGTGA TCGCCAGCTG CGTGCGGGAT GCCACGGGGC TTCGCGACGC ACTCAAGCTG 
    GTTGAGGCGT GCCGCTACGT ACAGGAATTT GGCAAGGAGG ATGACGCGGC GCGGCAAAAG 
    ATCCCCTTCC GCAACGTGTT CGCGCAGGAG TGCATTCGCC ATGCGCTGGA CCTGTCCAAT 
    GACGCCAACG GAAGCGAACT GATGCAGCAC CTGGTGCCGC TGCTGCGCTC GGGAACCAGG 
    CCGGTTACCA TGGGCGACAT TTTCTACGAC CCCACAACGA ACGAGCACGA CCTGTCAGAA 
    GTGCTGCTGC TGATTAGGGA GCTTTCCAGC GATATTGTAA CGGTGGCGTG CAACACGAAT 
    GGCGCGCGTG TCTCCCAGCG CATCATCGAT GTGCTTTGCA CACATGAGGA GTTTGATGTG 
    TACACGAGTG TGCTTGAGCC ATCGATTGTC GACGTTGCGA AGGACATTAA TGGCAACCAC 
    TCCCTGTCAA AGCTGATCAC GTCTGCCCGC TTTTGCCAGC TGGGCGACTC TGACAAGAGT 
    GCCTCCGGCG CTGCTGCGAT CTACGAGCGC ATTTTCCAGA AGATAGCGGA CAACTGCATC 
    GACATTTGCA AAAACCGTCA GGGGTGCTGT ATCATTCAAA AGTGCCTGCA ACACGCCCCC 
    CAGCCGTATC ACACTACCAT CATCAACACG GTACTCAACA ACTCGCTGAA GCTTGTGCAG 
    GATCCCTTTG GCAACTATGT TGTTCAGTTC ATTCTCGACA AACAGCAAGA CATCAACGGC 
    GCAAAGAAGG AGGACGCTGA CGACGACGCC ATGTCCACGG CACCGAACTA CACCAATCAA 
    ATTATTAGGC AGATGCTCCA TCACGTGGCC GAGCTCTCGT GCAACAAGTT TAGCAGCAAT 
    GTGATTGAGA AGTGCCTGAA GACGTCGTCG CCGGATGTGC GACAGCTGTT GGTCGACGAG 
    CTCACGGCGC CGCATGTCTT GCCTAAGCTG CTGACAGATA GCTTCGCGAA TTACGTTATT 
    CAGACCGCCA TTTCAACCGC CTCCGACGAT GGCCAACTGA CGCAGCTGCG CGACGCTATC 
    ATACCTCTGC AGAGTCTCCT GAAAAACTCC CCGTATGGCG TGAAGATCGA GTCGAAGCTG 
    TCCCGGCGCC ACCGTGAAGC GGCGCGGCGT CTGCTGAAGA AAAAGGAGGC CGCCACGACC 
    GTCACACAGG CCCCACCCGC ACAGCAAGAG CCGCTTCTTC CGCCATACAT GCCCCCACAA 
    GGAATGTCGG CCATTCCAAT GATGGCGCCA GACGCTTCCA TGGGTCAGCA AGTGTCCTTC 
    ACCAACCTGA ATACGGACAT GGCCTCCTTC TTGCAGCCAC AGCGGATGAT AGGCATGCCT 
    TTTGTGTTGC AAGGACAACA GATGATCAGC ATCCCCAATG CACCACCGCC GTCTTTCACC 
    ACGGGTATGA TCAACGGTAG CGGCCTGCCT GAGTACCGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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