ORF » Species Summary » Leishmania major strain Friedlin » LMJF_33_0812 cDNA ORF clone

LMJF_33_0812 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_33_0812 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_33_0812 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb04898 XM_001685781.1
Latest version!		 Leishmania major strain Friedlin beta tubulin partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_33_0812 ( XM_001685781.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001685833.1 Leishmania major strain Friedlin beta tubulin partial mRNA.
    Price & Availability↑
    CloneID OLb04898
    Clone ID Related Accession (Same CDS sequence) XM_001685781.1
    Accession Version XM_001685781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product beta tubulin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007285). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCGTGAGA TCGTTTCCTG CCAGGCCGGC CAGTGCGGCA ACCAGATCGG CTCTAAGTTT 
    TGGGAGGTGA TTGCCGACGA ACATGGTGTC GATCCGACTG GCTCCTACCA GGGTGACTCG 
    GATCTGCAGC TGGAGCGCAT CAACGTGTAC TTCGATGAGT CGGCGGGAGG CCGCTACGTG 
    CCGCGCGCCG TGCTGATGGA CCTCGAGCCC GGCACTATGG ACTCCGTTCG CGCCGGCCCG 
    TACGGCCAGC TGTTCCGCCC GGACAACTTC ATCTTTGGTC AGTCCGGCGC TGGCAACAAC 
    TGGGCCAAGG GCCACTACAC TGAGGGTGCG GAGCTGATCG ACTCCGTGCT TGATGTGTGC 
    CGCAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTGTCTCA CTCCCTCGGC 
    GGCGGCACGG GCTCCGGCAT GGGCACGCTG CTCATTTCCA AGCTGCGCGA GGAGTACCCG 
    GACCGGATCA TGATGACCTT CTCCGTCATC CCGTCCCCCC GCGTGTCGGA TACCGTTGTG 
    GAGCCGTACA ACACGACCCT CTCTGTGCAC CAGCTCGTGG AGAACTCCGA CGAGTCCATG 
    TGCATCGACA ACGAGGCGCT GTACGATATT TGCTTCCGCA CGCTGAAGCT GACGACGCCG 
    ACGTTCGGTG ACCTGAACCA CCTCGTCGCC GCTGTGATGT CTGGCGTGAC CTGCTGCCTG 
    CGCTTCCCTG GCCAGCTGAA CTCTGACCTG CGCAAGCTTG CCGTGAACCT CGTGCCGTTC 
    CCGCGCCTGC ACTTCTTCAT GATGGGCTTC GCGCCGCTGA CGAGCCGCGG CTCGCAGCAG 
    TACCGCGGCC TGTCCGTCGC GGAGCTGACG CAGCAGATGT TCGACGCCAA GAACATGATG 
    CAGGCCGCCG ACCCGCGCCA CGGCCGCTAC CTCACCGCGT CCGCGCTGTT CCGCGGCCGC 
    ATGTCGACCA AGGAGGTGGA CGAGCAGATG CTGAACGTGC AGAACAAGAA CTCCAGCTAC 
    TTCATCGAGT GGATCCCGAA CAACATCAAG TCGTCCATCT GCGATATCCC GCCCAAGGGT 
    CTCAAGATGT CTGTCACCTT CATCGGCAAC AACACCTGCA TCCAGGAGAT GTTCCGCCGC 
    GTCGGTGAGC AGTTCACGGG TATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGT 
    GAGGGCATGG ACGAGATGGA GTTCACGGAG GCCGAGTCCA ACATGAACGA CCTCGTCTCT 
    GAGTACCAGC AGTACCAGGA CGCCACCGTC GAGGAGGAGG GCGAGTACGA CGAGGAGGAG 
    GAAGCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001685833.1
    CDS1..1332
    Translation

    Target ORF information:

    RefSeq Version XM_001685781.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin beta tubulin partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001685781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCGTGAGA TCGTTTCCTG CCAGGCCGGC CAGTGCGGCA ACCAGATCGG CTCTAAGTTT 
    TGGGAGGTGA TTGCCGACGA ACATGGTGTC GATCCGACTG GCTCCTACCA GGGTGACTCG 
    GATCTGCAGC TGGAGCGCAT CAACGTGTAC TTCGATGAGT CGGCGGGAGG CCGCTACGTG 
    CCGCGCGCCG TGCTGATGGA CCTCGAGCCC GGCACTATGG ACTCCGTTCG CGCCGGCCCG 
    TACGGCCAGC TGTTCCGCCC GGACAACTTC ATCTTTGGTC AGTCCGGCGC TGGCAACAAC 
    TGGGCCAAGG GCCACTACAC TGAGGGTGCG GAGCTGATCG ACTCCGTGCT TGATGTGTGC 
    CGCAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTGTCTCA CTCCCTCGGC 
    GGCGGCACGG GCTCCGGCAT GGGCACGCTG CTCATTTCCA AGCTGCGCGA GGAGTACCCG 
    GACCGGATCA TGATGACCTT CTCCGTCATC CCGTCCCCCC GCGTGTCGGA TACCGTTGTG 
    GAGCCGTACA ACACGACCCT CTCTGTGCAC CAGCTCGTGG AGAACTCCGA CGAGTCCATG 
    TGCATCGACA ACGAGGCGCT GTACGATATT TGCTTCCGCA CGCTGAAGCT GACGACGCCG 
    ACGTTCGGTG ACCTGAACCA CCTCGTCGCC GCTGTGATGT CTGGCGTGAC CTGCTGCCTG 
    CGCTTCCCTG GCCAGCTGAA CTCTGACCTG CGCAAGCTTG CCGTGAACCT CGTGCCGTTC 
    CCGCGCCTGC ACTTCTTCAT GATGGGCTTC GCGCCGCTGA CGAGCCGCGG CTCGCAGCAG 
    TACCGCGGCC TGTCCGTCGC GGAGCTGACG CAGCAGATGT TCGACGCCAA GAACATGATG 
    CAGGCCGCCG ACCCGCGCCA CGGCCGCTAC CTCACCGCGT CCGCGCTGTT CCGCGGCCGC 
    ATGTCGACCA AGGAGGTGGA CGAGCAGATG CTGAACGTGC AGAACAAGAA CTCCAGCTAC 
    TTCATCGAGT GGATCCCGAA CAACATCAAG TCGTCCATCT GCGATATCCC GCCCAAGGGT 
    CTCAAGATGT CTGTCACCTT CATCGGCAAC AACACCTGCA TCCAGGAGAT GTTCCGCCGC 
    GTCGGTGAGC AGTTCACGGG TATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGT 
    GAGGGCATGG ACGAGATGGA GTTCACGGAG GCCGAGTCCA ACATGAACGA CCTCGTCTCT 
    GAGTACCAGC AGTACCAGGA CGCCACCGTC GAGGAGGAGG GCGAGTACGA CGAGGAGGAG 
    GAAGCCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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