ORF » Species Summary » Leishmania major strain Friedlin » cMDH cDNA ORF clone

cMDH cDNA ORF clone, Leishmania major strain Friedlin

The following cMDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cMDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb04094 XM_001684520.1
Latest version!		 Leishmania major strain Friedlin cytosolic malate dehydrogenase (cMDH), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    cMDH ( XM_001684520.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001684572.1 Leishmania major strain Friedlin cytosolic malate dehydrogenase (cMDH), partial mRNA.
    Price & Availability↑
    CloneID OLb04094
    Clone ID Related Accession (Same CDS sequence) XM_001684520.1
    Accession Version XM_001684520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product cytosolic malate dehydrogenase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007269). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCCGCTG TGAAGGTGGC CGTGACGGGT GCGGCTGGTC AGATCGGGTA TGCATTGGTA 
    CCGCTGATCG CGCGCGGCGC CCTCCTCGGG CCCACCACGC CTGTGGAGCT CCGCCTGCTG 
    GATATCGAGC CCGCTCTGAA GGCGCTTGCC GGCGTCGAAG CGGAGCTGGA AGACTGCGCC 
    TTCCCTCTCC TGGACAAGGT CGTCGTCACG GCAGACCCGC GCGTTGCCTT CGACGGGGTC 
    GCCATTGCCA TCATGTGCGG TGCCTTCCCG CGCAAGGCAG GGATGGAGCG CAAGGACCTC 
    CTCGAGATGA ACGCGCGCAT CTTCAAGGAG CAGGGTGAGG CCATCGCTGC TGTGGCCGCG 
    TCAGACTGCC GCGTGGTGGT TGTCGGCAAT CCGGCCAACA CAAACGCTCT CATCCTCCTC 
    AAGTCAGCTC AGGGGAAGTT GAACCCCCGT CATGTGACCG CCATGACCCG CCTCGATCAC 
    AACCGTGCCT TGTCTCTTCT GGCTCGCAAG GCCGGTGTGC CGGTGTCGCA GGTGCGCAAC 
    GTGATCATCT GGGGCAACCA TAGCTCTACA CAGGTCCCTG ACACTGACAG CGCTGTGATC 
    GGCACCACTC CGGCCCGCGA AGCGATCAAG GACGATGCAC TCGATGACGA CTTTGTGCAG 
    GTGGTGCGCG GGCGCGGCGC CGAAATCATC CAACTACGTG GTCTTTCCTC AGCCATGTCC 
    GCCGCCAAGG CCGCCGTAGA CCACGTACAT GACTGGATAC ATGGCACACC GGAGGGCGTG 
    TACGTTTCGA TGGGCGTGTA CTCGGATGAA AACCCGTACG GCGTGCCGAG CGGCCTCATC 
    TTCTCCTTTC CGTGCACGTG CCACGCCGGC GAGTGGACTG TTGTCTCGGG CAAGCTGAAT 
    GGGGACCTCG GGAAGCAGCG TCTCGCCAGC ACGATCGCCG AGCTGCAGGA GGAGAGGGCA 
    CAGGCAGGGC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001684572.1
    CDS1..975
    Translation

    Target ORF information:

    RefSeq Version XM_001684520.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin cytosolic malate dehydrogenase (cMDH), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001684520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCCGCTG TGAAGGTGGC CGTGACGGGT GCGGCTGGTC AGATCGGGTA TGCATTGGTA 
    CCGCTGATCG CGCGCGGCGC CCTCCTCGGG CCCACCACGC CTGTGGAGCT CCGCCTGCTG 
    GATATCGAGC CCGCTCTGAA GGCGCTTGCC GGCGTCGAAG CGGAGCTGGA AGACTGCGCC 
    TTCCCTCTCC TGGACAAGGT CGTCGTCACG GCAGACCCGC GCGTTGCCTT CGACGGGGTC 
    GCCATTGCCA TCATGTGCGG TGCCTTCCCG CGCAAGGCAG GGATGGAGCG CAAGGACCTC 
    CTCGAGATGA ACGCGCGCAT CTTCAAGGAG CAGGGTGAGG CCATCGCTGC TGTGGCCGCG 
    TCAGACTGCC GCGTGGTGGT TGTCGGCAAT CCGGCCAACA CAAACGCTCT CATCCTCCTC 
    AAGTCAGCTC AGGGGAAGTT GAACCCCCGT CATGTGACCG CCATGACCCG CCTCGATCAC 
    AACCGTGCCT TGTCTCTTCT GGCTCGCAAG GCCGGTGTGC CGGTGTCGCA GGTGCGCAAC 
    GTGATCATCT GGGGCAACCA TAGCTCTACA CAGGTCCCTG ACACTGACAG CGCTGTGATC 
    GGCACCACTC CGGCCCGCGA AGCGATCAAG GACGATGCAC TCGATGACGA CTTTGTGCAG 
    GTGGTGCGCG GGCGCGGCGC CGAAATCATC CAACTACGTG GTCTTTCCTC AGCCATGTCC 
    GCCGCCAAGG CCGCCGTAGA CCACGTACAT GACTGGATAC ATGGCACACC GGAGGGCGTG 
    TACGTTTCGA TGGGCGTGTA CTCGGATGAA AACCCGTACG GCGTGCCGAG CGGCCTCATC 
    TTCTCCTTTC CGTGCACGTG CCACGCCGGC GAGTGGACTG TTGTCTCGGG CAAGCTGAAT 
    GGGGACCTCG GGAAGCAGCG TCTCGCCAGC ACGATCGCCG AGCTGCAGGA GGAGAGGGCA 
    CAGGCAGGGC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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