ORF » Species Summary » Leishmania major strain Friedlin » LMJF_20_1200 cDNA ORF clone

LMJF_20_1200 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_20_1200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_20_1200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb02428 XM_001682820.1
Latest version!		 Leishmania major strain Friedlin putative calpain-like cysteine peptidase partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_20_1200 ( XM_001682820.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001682872.1 Leishmania major strain Friedlin putative calpain-like cysteine peptidase partial mRNA.
    Price & Availability↑
    CloneID OLb02428
    Clone ID Related Accession (Same CDS sequence) XM_001682820.1
    Accession Version XM_001682820.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative calpain-like cysteine peptidase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007261). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTCGATG AGTTTCGTGA GGACTCAGTA GAGCCGCTAC CAATGGATGC CAGTAAGAGC 
    TCACCAACGC CATGGGTGAA CTGGGGTCCG TCCATTGACG GCGAGGCTAC GCCGTGCTTC 
    AAGGGCGGTT TTCTCTATCG GATTATCGAT TCCAAGAAGA AAGTCTGGGC TCTGTATAAC 
    GATACGTTCG TCTACGAAAT GCATGCCACC TTTATTTTCG GGCCTGTGTC GAACATGGAG 
    CCCATCACCG CCTCGCTCTC TGCGTCGGAG AGCGGCGGCG AGGTGGATGA GAGGAACCGC 
    GTCGTCGCCA GCGTCGAGGA TAACGAGCTG ACGATCTCCA TGGTTGTCTA TCCGATGGAG 
    ACGATCGAGT TCGCCCGAGG CGACAAGTCG AAGTACAGAT GTCACTTCGA CGGCAAGCCG 
    CTCTCAAAAG AGTACCTTCA ACGCGAAATA GCCTTGGCAA ACACCGAGCT AGAGAGGAAC 
    AAGATGACGC TCGCGAGGTT CACCCACACC TCCACAGATC CTGAGGATGT GCTGGCGTTG 
    TGCAAAAAAC ACCGTTTGGT GTTCATAGAC CCCTCCTTCC CTCCTGGCCA GCACTCGCTT 
    GACGGGGATC GAGGTCTGAT TGCGCCAAGC GGGTGGAGGC GACCGGAGGC GTATCTGCCT 
    CTCGCGTTGA AGGGTCAAAT TCGTCTGTTT CGGCATCGTG TGACTCCCTC TGCCACACAG 
    CGTGGCGAAT TGGGCAATTC GTGGGTGGTG AGCGCCATGG CGGCGCTGGC GGAACGCCCG 
    GACTACGTCA AGGATATGTT TCGGCACCCC ATCAGCTCCG AAGAGACGCA GCGAGACGAG 
    GCGGTTGGGG CGTACCGGGT TTGGCTGAAC CAAGACGGTC TCTGGCGGTC GGTGCTCGTC 
    GACAGCTATC TGCCCGTGGT GGGCAAGCAG CAGCGCTACG CCCGCAGCGC CAACGGGCCG 
    TGCGAGATGT GGGTGTCTTA TCTCGAGAAG GTGTATGCGA AGCGTTATCA CAGCTACAGC 
    AACATCGCCG GCGGCGATCC GCTCTTTGCA ATTCGCGACT TCACCGGATT TCCTACGTCG 
    CGGCTGGACG CTCGCTTTCG CGAGTGTGCC ACGGACCCGG TGAAGAGCGA TATGTTTTTC 
    CTTCGCATGG TACGCGACTA CGAGAACGGG CACCTTGTGC TACTGAGCAC TCCCGGCAGC 
    GGTGACCCGC GCTCTGCCCA TAGCACATAC AAGGAGAAGG GCATTTTTGT GGGCTACGCC 
    TATGCAGTGC TAGAAGCTCG CGCGATCGAA TCGCCGACAT TGCGGCGGAA GAAGGTACCA 
    CCGCTGCGGC TGCTGCGGCT GCGCAACGCG TGGGAGGCGG CGACCAAGTG GAGTGGCAAG 
    TGGTCTGACG ACTCGTCGAT GTGGCAGGCG CACCCGGAAG CGTGTGTTGC GTTTCCGCAG 
    CACAGCGGGA AGGATGGAAC GTTCATCATG GAGTGGTCGG AGGTGCTGGG GATCTTTGTC 
    GGCTGCGGTG TTGTGTTCAA CCACTTCGGC TACACGGACT ACCGCATCCC CTTCGAGTTC 
    CGGGGCTCTG TGCCGGACCT GTGCCTCGAG ATTCACGTGA CAGAGCCGAC AACGTTGACG 
    CTCATACTGT CGCAGCCAGA CAGCAAAGGC ACGGTGAGGG AGTCGGAGGA CTACAACCCT 
    GTCATGATTT CCATAGCGTG CGCCTCCTCC GCCAAGACGA GCCCGGTTTC CCAGAGTGGC 
    AGCAAGCGTA GCAGCGGGAC CTCCAGTCAC TCCATGATGA CGGCCAGTAC GCAGCTGGTG 
    GCGCGTGATT ACTCTCTCCT CGTCAATTCC TCTGCGGACG CCGAGGCGCC GTCAAAGAGC 
    TTCACGTTCT TGCAGGGCCG CGACGTAAGC GCCATATGCA CGCTCGTCCC AGAGCAGTCG 
    CCGTACCTCG TCGTGCCACG TCTGCTCGTG CAGCAGTCTA GCGGCAGCAA CGGCACGAGG 
    GCGTCCAGTG CGGCTGTGGC CTCGGCATCA AACAGCGCTG TGACGTGGCC ATGCGTGCTC 
    GGTGTTCTTT CGCAGCTTGC CTTCACCCCA AACGGACAGG CACACGTTCA TCTGCGCAAG 
    TTGCCAGCGA ACAGTCTCGT CTTCGACAAC TACCTCAGCT TCGCCAACGA CTCCGTGGAG 
    GTCGCGAAGA CCTTCTACGT GAAACGCTCC GGCACCAGTG TGGTGGAGCG CAGCGCCTCA 
    GAACTACAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001682872.1
    CDS1..2232
    Translation

    Target ORF information:

    RefSeq Version XM_001682820.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative calpain-like cysteine peptidase partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001682820.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTCGATG AGTTTCGTGA GGACTCAGTA GAGCCGCTAC CAATGGATGC CAGTAAGAGC 
    TCACCAACGC CATGGGTGAA CTGGGGTCCG TCCATTGACG GCGAGGCTAC GCCGTGCTTC 
    AAGGGCGGTT TTCTCTATCG GATTATCGAT TCCAAGAAGA AAGTCTGGGC TCTGTATAAC 
    GATACGTTCG TCTACGAAAT GCATGCCACC TTTATTTTCG GGCCTGTGTC GAACATGGAG 
    CCCATCACCG CCTCGCTCTC TGCGTCGGAG AGCGGCGGCG AGGTGGATGA GAGGAACCGC 
    GTCGTCGCCA GCGTCGAGGA TAACGAGCTG ACGATCTCCA TGGTTGTCTA TCCGATGGAG 
    ACGATCGAGT TCGCCCGAGG CGACAAGTCG AAGTACAGAT GTCACTTCGA CGGCAAGCCG 
    CTCTCAAAAG AGTACCTTCA ACGCGAAATA GCCTTGGCAA ACACCGAGCT AGAGAGGAAC 
    AAGATGACGC TCGCGAGGTT CACCCACACC TCCACAGATC CTGAGGATGT GCTGGCGTTG 
    TGCAAAAAAC ACCGTTTGGT GTTCATAGAC CCCTCCTTCC CTCCTGGCCA GCACTCGCTT 
    GACGGGGATC GAGGTCTGAT TGCGCCAAGC GGGTGGAGGC GACCGGAGGC GTATCTGCCT 
    CTCGCGTTGA AGGGTCAAAT TCGTCTGTTT CGGCATCGTG TGACTCCCTC TGCCACACAG 
    CGTGGCGAAT TGGGCAATTC GTGGGTGGTG AGCGCCATGG CGGCGCTGGC GGAACGCCCG 
    GACTACGTCA AGGATATGTT TCGGCACCCC ATCAGCTCCG AAGAGACGCA GCGAGACGAG 
    GCGGTTGGGG CGTACCGGGT TTGGCTGAAC CAAGACGGTC TCTGGCGGTC GGTGCTCGTC 
    GACAGCTATC TGCCCGTGGT GGGCAAGCAG CAGCGCTACG CCCGCAGCGC CAACGGGCCG 
    TGCGAGATGT GGGTGTCTTA TCTCGAGAAG GTGTATGCGA AGCGTTATCA CAGCTACAGC 
    AACATCGCCG GCGGCGATCC GCTCTTTGCA ATTCGCGACT TCACCGGATT TCCTACGTCG 
    CGGCTGGACG CTCGCTTTCG CGAGTGTGCC ACGGACCCGG TGAAGAGCGA TATGTTTTTC 
    CTTCGCATGG TACGCGACTA CGAGAACGGG CACCTTGTGC TACTGAGCAC TCCCGGCAGC 
    GGTGACCCGC GCTCTGCCCA TAGCACATAC AAGGAGAAGG GCATTTTTGT GGGCTACGCC 
    TATGCAGTGC TAGAAGCTCG CGCGATCGAA TCGCCGACAT TGCGGCGGAA GAAGGTACCA 
    CCGCTGCGGC TGCTGCGGCT GCGCAACGCG TGGGAGGCGG CGACCAAGTG GAGTGGCAAG 
    TGGTCTGACG ACTCGTCGAT GTGGCAGGCG CACCCGGAAG CGTGTGTTGC GTTTCCGCAG 
    CACAGCGGGA AGGATGGAAC GTTCATCATG GAGTGGTCGG AGGTGCTGGG GATCTTTGTC 
    GGCTGCGGTG TTGTGTTCAA CCACTTCGGC TACACGGACT ACCGCATCCC CTTCGAGTTC 
    CGGGGCTCTG TGCCGGACCT GTGCCTCGAG ATTCACGTGA CAGAGCCGAC AACGTTGACG 
    CTCATACTGT CGCAGCCAGA CAGCAAAGGC ACGGTGAGGG AGTCGGAGGA CTACAACCCT 
    GTCATGATTT CCATAGCGTG CGCCTCCTCC GCCAAGACGA GCCCGGTTTC CCAGAGTGGC 
    AGCAAGCGTA GCAGCGGGAC CTCCAGTCAC TCCATGATGA CGGCCAGTAC GCAGCTGGTG 
    GCGCGTGATT ACTCTCTCCT CGTCAATTCC TCTGCGGACG CCGAGGCGCC GTCAAAGAGC 
    TTCACGTTCT TGCAGGGCCG CGACGTAAGC GCCATATGCA CGCTCGTCCC AGAGCAGTCG 
    CCGTACCTCG TCGTGCCACG TCTGCTCGTG CAGCAGTCTA GCGGCAGCAA CGGCACGAGG 
    GCGTCCAGTG CGGCTGTGGC CTCGGCATCA AACAGCGCTG TGACGTGGCC ATGCGTGCTC 
    GGTGTTCTTT CGCAGCTTGC CTTCACCCCA AACGGACAGG CACACGTTCA TCTGCGCAAG 
    TTGCCAGCGA ACAGTCTCGT CTTCGACAAC TACCTCAGCT TCGCCAACGA CTCCGTGGAG 
    GTCGCGAAGA CCTTCTACGT GAAACGCTCC GGCACCAGTG TGGTGGAGCG CAGCGCCTCA 
    GAACTACAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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