ORF » Species Summary » Leishmania major strain Friedlin » EF1G cDNA ORF clone

EF1G cDNA ORF clone, Leishmania major strain Friedlin

The following EF1G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EF1G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb00727 XM_001681219.1
Latest version!		 Leishmania major strain Friedlin elongation factor-1 gamma (EF1G), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    EF1G ( XM_001681219.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001681271.1 Leishmania major strain Friedlin elongation factor-1 gamma (EF1G), partial mRNA.
    Price & Availability↑
    CloneID OLb00727
    Clone ID Related Accession (Same CDS sequence) XM_001681219.1
    Accession Version XM_001681219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product elongation factor-1 gamma
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007250). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACCTACA AGCTCCTCGC CCCCCTCCAT CCGGAGAGCG CGCGCGCCCA GAAGATAATG 
    GTTGCCGCCG CGTACGCCAA CGTCGACGTC GAGCTCAAGG TGTGCCAGTA CGGCCAGGAG 
    AACGAGACGC CCGAGTTTGC GCGCAACTGC AGCCCGTGCA TGCGCTTCCC CTCCATGCAG 
    ACGGAGGAGG GCTACCTCTT CGAGTCCAAC GCCATCATGC GCCACATCGC CCGTGTGGAG 
    AAGAGCGGCG CGAAGCTGTA CGGCGCCACA CCCTTCGAGA GCAGCCAGGT CGACATGTGG 
    CTTGACTTCG CCTCCAGCGA GCTCGATGCG CACAACACGC CGTACCTGAT GGAGACCTTC 
    GCCGGCATTC CGGCCCCGGA GAGCGCCATG GCGACGCTGG AGGAGAGCCT TGCGGGCCTG 
    GAGCTGTGGC TGGAGACCCG CACCTTCCTC GTCGGCGAGC GCATGACCGT GGCCGACATC 
    TCCGTCGCCT TTGCGCTGCA GTGGGTGTAC CGCATGAACG TGAAGCACGG CGAGGAGCTG 
    ACGAAGAAGT ACCGCAACGC GTACCGCCTG TACAACACGG TGATGCAGCA GCCCAAGACG 
    GTGGAGGTGC TGAAGCAGTG GGGCGCGACG TTCGGCCCGG CGAAGGCGCC GAAGAAGGCC 
    GCGGAGGCGA AGCCCAAGGA GGGGAAGAAG CCGAAGGAGG CGGTGGAGGA CGAGGAGGAG 
    CAGCCGGCGA AGGAGGAGAA GAAATCGAAC CCGCTGGACG CGCTGCCGCC GAGCAGCTTC 
    GTGCTGGACG CCTACAAGCG CGAGTACAGC AACACCGACA CTCGAACGGT GGCGGCGCCG 
    TACTTCTTCG AGCACTACGA TGCGGAGGGC TACACGTGCT TCTGGGCACG CTACAAGTAC 
    AACGAGGATA ACAAGAAGCA GTTCATGACA GCCAACCTTG TGCGCGGCTG GTTCCAGCGC 
    ATGGAGCACG TGCGCAAGTA CGCCTTCGGC GTCGCGCTTA TCATCGGCGA GGACGCAGCG 
    CACGAGCTGG TGAGCTTCTG GGTGTTCCGC GGCAAGGGCA TGCCGGAGAT CGTGAGCGAA 
    GTGGTGGACA CGGAGTTGTT CGAGTGGGAG GAGATCAAGG ACGTGCAGGC GGCGAAGGAA 
    AAGATCACGG ACTACCTATG CTGGGAGGGC CCGACGATCC CGCGCCCGGT GCTGGAGGGC 
    CGCTGCTTCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001681271.1
    CDS1..1215
    Translation

    Target ORF information:

    RefSeq Version XM_001681219.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin elongation factor-1 gamma (EF1G), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001681219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACCTACA AGCTCCTCGC CCCCCTCCAT CCGGAGAGCG CGCGCGCCCA GAAGATAATG 
    GTTGCCGCCG CGTACGCCAA CGTCGACGTC GAGCTCAAGG TGTGCCAGTA CGGCCAGGAG 
    AACGAGACGC CCGAGTTTGC GCGCAACTGC AGCCCGTGCA TGCGCTTCCC CTCCATGCAG 
    ACGGAGGAGG GCTACCTCTT CGAGTCCAAC GCCATCATGC GCCACATCGC CCGTGTGGAG 
    AAGAGCGGCG CGAAGCTGTA CGGCGCCACA CCCTTCGAGA GCAGCCAGGT CGACATGTGG 
    CTTGACTTCG CCTCCAGCGA GCTCGATGCG CACAACACGC CGTACCTGAT GGAGACCTTC 
    GCCGGCATTC CGGCCCCGGA GAGCGCCATG GCGACGCTGG AGGAGAGCCT TGCGGGCCTG 
    GAGCTGTGGC TGGAGACCCG CACCTTCCTC GTCGGCGAGC GCATGACCGT GGCCGACATC 
    TCCGTCGCCT TTGCGCTGCA GTGGGTGTAC CGCATGAACG TGAAGCACGG CGAGGAGCTG 
    ACGAAGAAGT ACCGCAACGC GTACCGCCTG TACAACACGG TGATGCAGCA GCCCAAGACG 
    GTGGAGGTGC TGAAGCAGTG GGGCGCGACG TTCGGCCCGG CGAAGGCGCC GAAGAAGGCC 
    GCGGAGGCGA AGCCCAAGGA GGGGAAGAAG CCGAAGGAGG CGGTGGAGGA CGAGGAGGAG 
    CAGCCGGCGA AGGAGGAGAA GAAATCGAAC CCGCTGGACG CGCTGCCGCC GAGCAGCTTC 
    GTGCTGGACG CCTACAAGCG CGAGTACAGC AACACCGACA CTCGAACGGT GGCGGCGCCG 
    TACTTCTTCG AGCACTACGA TGCGGAGGGC TACACGTGCT TCTGGGCACG CTACAAGTAC 
    AACGAGGATA ACAAGAAGCA GTTCATGACA GCCAACCTTG TGCGCGGCTG GTTCCAGCGC 
    ATGGAGCACG TGCGCAAGTA CGCCTTCGGC GTCGCGCTTA TCATCGGCGA GGACGCAGCG 
    CACGAGCTGG TGAGCTTCTG GGTGTTCCGC GGCAAGGGCA TGCCGGAGAT CGTGAGCGAA 
    GTGGTGGACA CGGAGTTGTT CGAGTGGGAG GAGATCAAGG ACGTGCAGGC GGCGAAGGAA 
    AAGATCACGG ACTACCTATG CTGGGAGGGC CCGACGATCC CGCGCCCGGT GCTGGAGGGC 
    CGCTGCTTCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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