ORF » Species Summary » Leishmania major strain Friedlin » LMJF_05_0140 cDNA ORF clone

LMJF_05_0140 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_05_0140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_05_0140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb00133 XM_001687438.1
Latest version!		 Leishmania major strain Friedlin putative nucleolar RNA helicase II partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_05_0140 ( XM_001687438.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001687490.1 Leishmania major strain Friedlin putative nucleolar RNA helicase II partial mRNA.
    Price & Availability↑
    CloneID OLb00133
    Clone ID Related Accession (Same CDS sequence) XM_001687438.1
    Accession Version XM_001687438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product putative nucleolar RNA helicase II
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007246). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCGCGTC GCGCGCGCGA GGAGGATGGT GAGGAGAAAC CGCACCGCGT CACGAAGCTC 
    GCCAGCAGCA GCGCCGACGA TGAGCCGGTG AAGGCGTCGA AGATCTCCGC CAAGGCGTCG 
    GACAACGACA CCGGCGCTGC TGCAGAGAAA AGGAAGCGCA CGGAGGGGAG CTCCAAGGTT 
    TCTGCCACTT CGGCAACGGC ACCGGCGGAG AAGAAGGACG AGGAGGTGCC GAACAATGGC 
    TGCGCCGCCA CAGCGCGCCC GTTCTCGGAG TTTGAGATGA ACCCCATCGT TGTCAAGGCG 
    CTGCAGTCAC GCGGCATCGA GTCCATGTTT CCTGTGCAGG CTCTCACCTT CAACGCCATC 
    ATGAGGAACA CGGACGTCCT CGTGCAGGCC CGCACCGGCA GCGGCAAGAC ACTCGCGTTC 
    GGTATCCCGA TCGTGGAGCG GCTGCTGAAG CTGCCGAGCC ACCTCACCCG CGGCCGCGGC 
    CCAGCTGCCG TGATCTTCTG CCCTACTCGC GAGCTAGCCA TCCAGGTGCA GGATGTGCTC 
    TGCGGGATCA GCTGCGGGCT CGTCGTGACG GCCCTGTACG GCGGTGTCGC CTACGCGAAT 
    CAGGAGCGCG TGCTGCGCAG CGGCGTCGAC ATTGTGGTGG CCACCCCCGG CCGCGCGAAG 
    GACTTTCTAG AGAAGGGCAC GCTGCATTTT GACCGCGTTG TGATGGCGTG CCTGGACGAG 
    GCGGACCACA TGCTGGACAT CGGCTTCAAG GACGACATTG AGTTGCTGCT CTCGCAGGTG 
    GCCGAGCAGA ACGGCTCTGT CGGCGCTGAG AGGCCAGCTC ATCAGACGCT GCTGTTCTCC 
    GCGACTGTGC CGGAGTGGGT GCACACGTGC TCCTTCATCG CCAAGGACAA GGAGTTCATC 
    GACATGGTCG GCAAGGAGGC GGTGAGGACA GCGAGCACGA TCAAGTTCTA TCGCAGAAAA 
    TGCAACTTCA GCGAGATCTC TTCTATGCTC GCTGATCTCA TCAAGGTGTA CAGCGGTGCC 
    CACGGCCGCA CCCTCGTCTT CACCAACACG AAGAAGGACT GCCACGACCT GTCCATCAAC 
    AACACCAAAC TGGACTCGCA GTGCCTGCAC GGTGATATGC AGCAGGAGCA GCGCGAGAGT 
    ACCATGAAGA GCTTCCGCGA CAACAAGTTC AGCGTCCTCA TCGCCACCGA CGTGGCGGCC 
    CGCGGCCTCG ACTTGCCCAT GGTGGACCTG GTTATCCAGT GCGCCCCGCC GAGCGACATC 
    GACGCTTTCA TTCACCGAGC CGGCCGTACG GGACGCGCTG GCCGCAAGGG TGTGTGCGTG 
    CTGCTCTACC AGCCGCGCGA GGAGTACGTT GTCGAGCGCA TCGAGCGCCA CGCGAAGATG 
    AAGTTCGACG TCCTGCCTGC CCCCACGCGT GAGGAGATTC TCAAGGCCGT CGCCCGTGAT 
    GCCGCTGAGG ACCTGGCGCG CGTCGAGCGC CGTGCCACCG ACCTCTTCAT GGAGCAGGCG 
    GCCGAGCTGC TAAAGGACGC GGACCCAGTC GAAATCCTGG CCTCGGCGCT CGCCGTCATG 
    AGCGGCTACA CGTCGAACAT CACCGCGCGC GGTCTCATTA CTGGCACGCC AGGGTATGTG 
    ACGGTGCAGA TGACGTCGGA TCGACCGCTG CCCGTTCCGG TGTACTGCAG CATCCTCCGA 
    AACAACCTCG GCGATGACAC GTTCATGCGG TGCCGCGACA TTACACTGTT GCAGGACGAC 
    CCGGGCTGCG TCTTCGACGT GCTGGAGCAG TTTGCTGAGC GTGTCATGAG CACACCGATG 
    CGCGGTATCA CCTTCCAGCG GATCGAGGCC CTTCCTCCGA TTATCGCGCG CGACCTCAAC 
    AGCAGCCGCG GTGGTCGCGG TGGCTTTAAC TACGGCGGCG GTCGCGGCGG CTTTAACTAC 
    GGCGGTGGGC GTGGTGGCTT CGGCGGCTAC GGTGGGAATC GTGGCAGCGG CCGAGGCAGC 
    GGCCGAGGCG GTGGCCGTGT GGGCGGTTTC CAGCGCCGCT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001687490.1
    CDS1..2025
    Translation

    Target ORF information:

    RefSeq Version XM_001687438.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin putative nucleolar RNA helicase II partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001687438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCGCGTC GCGCGCGCGA GGAGGATGGT GAGGAGAAAC CGCACCGCGT CACGAAGCTC 
    GCCAGCAGCA GCGCCGACGA TGAGCCGGTG AAGGCGTCGA AGATCTCCGC CAAGGCGTCG 
    GACAACGACA CCGGCGCTGC TGCAGAGAAA AGGAAGCGCA CGGAGGGGAG CTCCAAGGTT 
    TCTGCCACTT CGGCAACGGC ACCGGCGGAG AAGAAGGACG AGGAGGTGCC GAACAATGGC 
    TGCGCCGCCA CAGCGCGCCC GTTCTCGGAG TTTGAGATGA ACCCCATCGT TGTCAAGGCG 
    CTGCAGTCAC GCGGCATCGA GTCCATGTTT CCTGTGCAGG CTCTCACCTT CAACGCCATC 
    ATGAGGAACA CGGACGTCCT CGTGCAGGCC CGCACCGGCA GCGGCAAGAC ACTCGCGTTC 
    GGTATCCCGA TCGTGGAGCG GCTGCTGAAG CTGCCGAGCC ACCTCACCCG CGGCCGCGGC 
    CCAGCTGCCG TGATCTTCTG CCCTACTCGC GAGCTAGCCA TCCAGGTGCA GGATGTGCTC 
    TGCGGGATCA GCTGCGGGCT CGTCGTGACG GCCCTGTACG GCGGTGTCGC CTACGCGAAT 
    CAGGAGCGCG TGCTGCGCAG CGGCGTCGAC ATTGTGGTGG CCACCCCCGG CCGCGCGAAG 
    GACTTTCTAG AGAAGGGCAC GCTGCATTTT GACCGCGTTG TGATGGCGTG CCTGGACGAG 
    GCGGACCACA TGCTGGACAT CGGCTTCAAG GACGACATTG AGTTGCTGCT CTCGCAGGTG 
    GCCGAGCAGA ACGGCTCTGT CGGCGCTGAG AGGCCAGCTC ATCAGACGCT GCTGTTCTCC 
    GCGACTGTGC CGGAGTGGGT GCACACGTGC TCCTTCATCG CCAAGGACAA GGAGTTCATC 
    GACATGGTCG GCAAGGAGGC GGTGAGGACA GCGAGCACGA TCAAGTTCTA TCGCAGAAAA 
    TGCAACTTCA GCGAGATCTC TTCTATGCTC GCTGATCTCA TCAAGGTGTA CAGCGGTGCC 
    CACGGCCGCA CCCTCGTCTT CACCAACACG AAGAAGGACT GCCACGACCT GTCCATCAAC 
    AACACCAAAC TGGACTCGCA GTGCCTGCAC GGTGATATGC AGCAGGAGCA GCGCGAGAGT 
    ACCATGAAGA GCTTCCGCGA CAACAAGTTC AGCGTCCTCA TCGCCACCGA CGTGGCGGCC 
    CGCGGCCTCG ACTTGCCCAT GGTGGACCTG GTTATCCAGT GCGCCCCGCC GAGCGACATC 
    GACGCTTTCA TTCACCGAGC CGGCCGTACG GGACGCGCTG GCCGCAAGGG TGTGTGCGTG 
    CTGCTCTACC AGCCGCGCGA GGAGTACGTT GTCGAGCGCA TCGAGCGCCA CGCGAAGATG 
    AAGTTCGACG TCCTGCCTGC CCCCACGCGT GAGGAGATTC TCAAGGCCGT CGCCCGTGAT 
    GCCGCTGAGG ACCTGGCGCG CGTCGAGCGC CGTGCCACCG ACCTCTTCAT GGAGCAGGCG 
    GCCGAGCTGC TAAAGGACGC GGACCCAGTC GAAATCCTGG CCTCGGCGCT CGCCGTCATG 
    AGCGGCTACA CGTCGAACAT CACCGCGCGC GGTCTCATTA CTGGCACGCC AGGGTATGTG 
    ACGGTGCAGA TGACGTCGGA TCGACCGCTG CCCGTTCCGG TGTACTGCAG CATCCTCCGA 
    AACAACCTCG GCGATGACAC GTTCATGCGG TGCCGCGACA TTACACTGTT GCAGGACGAC 
    CCGGGCTGCG TCTTCGACGT GCTGGAGCAG TTTGCTGAGC GTGTCATGAG CACACCGATG 
    CGCGGTATCA CCTTCCAGCG GATCGAGGCC CTTCCTCCGA TTATCGCGCG CGACCTCAAC 
    AGCAGCCGCG GTGGTCGCGG TGGCTTTAAC TACGGCGGCG GTCGCGGCGG CTTTAACTAC 
    GGCGGTGGGC GTGGTGGCTT CGGCGGCTAC GGTGGGAATC GTGGCAGCGG CCGAGGCAGC 
    GGCCGAGGCG GTGGCCGTGT GGGCGGTTTC CAGCGCCGCT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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