ABCA1 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCA1
Entrez Gene ID	12981568
Full Name	putative ATP-binding cassette subfamily A,member 1
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_003721588.1	XP_003721636.1	putative ATP-binding cassette subfamily A,member 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ABCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb07405	XM_003721588.1 Latest version!	Leishmania major strain Friedlin putative ATP-binding cassette subfamily A,member 1 (ABCA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCA1 ( XM_003721588.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_003721636.1 Leishmania major strain Friedlin putative ATP-binding cassette subfamily A,member 1 (ABCA1), partial mRNA.

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CloneID	OLb07405
Clone ID Related Accession (Same CDS sequence)	XM_003721588.1
Accession Version	XM_003721588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	putative ATP-binding cassette subfamily A,member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007244). COMPLETENESS: incomplete on both ends.

ATGTCGCCGC CGTCAGCCGT CGCCTCTCTT AGCTCAGGTG GACAGCTGCC CTCTGCAGCG 
CCTGCGGCGG AGCGTCCGCA AGAGCAGTCA CGGGCACCCC CAGCGGCGAC GGCGCTCATC 
TCGACGAGCC AAGCGGCGCA CGACCCCGGC GCGGGCGCAC CTGGCTTATC GCATTTCGAT 
GTCGCTACCC CCCTCCTCCT TGAAGACCGA CGCTTCGCGC GGCGGGACGC CGGCATCTGC 
GGCACCGACA GCTACACGAT GCACAGCGAT AGCACGCATA GCAGCAACCT TACTATGAGC 
TCCGCCTACG CCGATGCCGA GGGGCACAGG AATGCCGGCA ACGCCGCCGT GAACAGAGGC 
GGCAGCAGTA GCAGGTCTAT GGAGAGTGAT GTAGGCGACT TCGCCAGCGA GTATTACGGT 
AGCATGCCCA CCTCCGGCCT CGGACACGAC GGGCACGGCC GCGCCGACGA CGTCCACACG 
GGCAACCTGA GAGCCCCCTT CGACCGCAGC AGCGCACTAG ACTACTGGGA TACCGTGACC 
ACGACTTCGT TCTCGGAGTC CCATACACTG ACGACTAAGC ACGGCGGCGG CGCCGACGAC 
CTTGCAAGCC CGACGGCTGG CGGTGCCGGA TGGACTCATA AGGACTCCAG CACGTGGCTC 
GACCAACTCG GGGCGACTAT TTCTCGCGAT ATGATCGAGC GGCGCCGCCG CTGGGGCAGC 
GTGGTGCTGG AGATTGCGAT CCCGCTTGTG TGCACGGTCT GTGTGGTGGC GCTGTGGGCC 
GCCTTCGGCG TTACTTCCTC GCCGGACTAC AGCTCGCTGG CCTCTATTGG TGATAAAGGA 
TACAACTTCG CGGCTGGCAG GTACCTGGCG GAACTCTGCT ACAACGAGAC GTGGTTTGGG 
GAGCGGAACT ACATCGACGG ACTGCGCCCC TGCAGCAGCC TAGACGCTGC GTCACTGAGA 
TCAGTGATCT GCAACACGGT CGGCGAGGAG GAGCTGCCCG TGAAGGGGCT GTGCTACAGA 
CAAGGGGGCC GGGGGCTTGC TGACTTTCTC AGCGGTATGC GCAACGGCCT CGCGCGGGTG 
ATGCCGCTAG ACGACATCAT CGCCCGCCAG TGGATGGCCA AAAAGTTGTC CTTAACGGAT 
TCGGCGGCGA TGGCGCTGCT GATCGGCGCG GGGTTCTTCT CCCACACGCG CATCAACGCT 
ATCGCGTCGT CCGGCAAGCT GTACTTCGCC CCAAGCGAAA ACGTGCCACT CGACATGCTC 
CAGTACCTGT CCAACAACTC TAGGTTTTTC CAGTACGTGT ACAACGATAC CTTCGACACC 
GTCGAGGAGG CGCACCGCGT GATCAAGAGC GGCGAGCGTG GACCCACATG GGCTCTCGTG 
AACATCCGCC AGCTGGACGA GAACGGCTTG GACTTGTCTA TCGAAATGGC CTCGACAGCG 
CTGCCGGCGT TGACGGGGAT GGTGGACAAG GCGTACTCCG GCGGCGGGGG CTACGATCGA 
TCCAACATGT ATTACACGAG CGGCTACCCG TCCTTGATGG ACGCACTTGT GAAGTACTAC 
CTCATGTCCC TGTACGCGGC CGGCGCAACG CGCGCTGAAA CGGGCAGCGT TCAGGCCCTG 
GCCGAGAGGC GGGCGGAGCA ACAGCAGCGG CAACAGGCGC AGGCAGCGGC TGACGACTCG 
GTGGACGTGG TTCTGGATAC GCACAAACTG CTCAACGCTG TGCGGGCGGC GCGGGAACCA 
GTGGGCAGCG GTACCCAGTT CTTCACCGAT GTCGCCGAGC TGGGCAACTC CTCGGCGGCC 
GTGACGTCAC AGAAAGCCTC TCGCATCCTG GCGACGAGCG CGGCCCTTCG CGCGAAGACG 
AGCTTCACGA TCTTCCTGGG CAGCATGCCA TGGGTGCCCT TCAAGACGAG CCAGGTGCTG 
ACTTCTGCGA ACACCATCAT CGGCTTCGTC ATTGTGATGG CGTTTCTCTA CCCTGTCTCG 
CAGTTGACGC GGCGGCTCGT GCTAGAGAAG GAGCGGCGCG TGCGGGAGGC GACGCTGATC 
ATGGGGCTCA GGCCGGTATA TTTTTGGTGC AGCTGGTTCC TTTACAGCGT CGCCCTCATG 
CTCACGATCT CCCTCTTCAT GACGCTGCTG ATGTGCACAA CGTTCCTGAC GAAGAGCGAT 
GCCTCATGCG TGCTTCTTGT CCTTGTCATG TTCTCCCTCA CGTGCGTGCC GCTGTCGGGG 
CTGATGGCCA CTCTCTACAA CACGTCGCAC ATGGCGGTTC TCATGACGCC ACTCCTCTAC 
TTTGCCATGT CTCTGCTGGT CTTCGCGATC TATTCGGCCA GTCCGGCCGT CTACCTCGGC 
CTCAGCATCT TCTCGCCCAC CTGCTTCGCG ATCATCCTGC AGATCATGCT CGCCCGGGAG 
AGCGGCGACG GCTTTGCGAC GAGGATCTTT CTGGACCCGA ACGACAACCC CAACACCGCC 
ACGCTCTTGG GATTCCTCGT CCTGGACTTT GGTGTGTATC TGCTGCTCAT GTTCTACCTC 
GATGCGGTGC TGCCCAAGGC GGTTGGTGTG TCGAAGCACC CGCTCTACTT CATCCTAGAA 
CCGGCCCGGC ATTGCCGTCG CAGGCGTGCG GAAAGGGCGT GGCAGGCGGA ACAGCGGCGG 
CGTCAAGCGG CGTCTGCCAC CGACGGCGGC AGTGGTGCAA GGGAGGCCCC GCAGCCGCCA 
CCGACGCAGA GGAGGAGGAC TCATGATCCC AAAGAGGGGA CGCACGGCGG CAGCGATGGC 
TCGTGCTGCA CTGGGTACAA CGTTGCAACG GATGACGCCG ACGAGGACCT GTGCGACGGC 
GAGGACGCCC GTGATCCGAA CGGCGTGTAC GAGGCGGAGA CCTTCACGCC GGAACAACTG 
TCCGTGCGCA TTATCGGCCT CCGCAAGTGC TTCGAGCACG GTGGCCGCCG CTTCGTTGCG 
GTGCGGAACT TCTGCTGGCA GCTGCCGAAC GCTGGCATCT CCGTGCTGCT GGGGCACAAC 
GGCGCCGGCA AGTCGACGCT GATCAACATG ATGACGGGCA TGCTCTACCC GGATGGCGGG 
GACTGCTACA TCGGCGGCCA CTCCATCCGC TCCGATCTGG CGCGCGCCCG CCACCAGATC 
GGTTTCTGTC CACAGCACAA CATTCTCTGG CCGGAGCTGA CGTGCCGTGA GCACCTCGAG 
TTCTTTGCGC GGCTGAAGGG GCTGCGCGGG GCTGCGCTGG AGGATGCTGT GATGTACACC 
CTCAAGGAGG TGGATCTTGT GGCGAGGCAG AGCGACACGA CGCGCGTTCT CTCGGGTGGC 
ATGAAGCGGA AGCTCTCGGT CGCCATCGCG TTTGTAGGTG GCAGCAGCCT CGTCTTCCTC 
GATGAGCCGA CCGCCGGCAT GGACGTGGCG GCGCGGCGGC ACACGTGGAG GCTGTTGCTG 
CGCATGTCGC GCAGCCGGAG CGTACTGCTG ACCACGCACT TTATGGACGA GGCGGACTTG 
CTCGGTGACC GGGTGGCCAT CATGAGCCGT GGCCAGCTTA AGTGCGCTGG CAGCTCGCTC 
TTCTTGAAGT CGCGCCTGGG TATCGGGTAC AACATCACCA TCTCTGTCGA CGAGCTGCTA 
CGCGTCCGTG ACCTCGATGT CTTTTTGCAG CAGCACGTGC CGCAGGCGGA GCGCTTGACA 
AGCAGTGGCG GCGAGGTCGG CTACCGCCTG CCTGCGAAGC ATGTGGAGCG CTTCCCAGGA 
CTGCTGAGCG CGCTCGACAG AGTTGGGCCG CTGATCGGCA TTCGTGGCTA CGCCATCTCT 
CCCACAACGC TGGAGGAGGT GTTTCTGACG ATCGCCCGCG ACGCCGCGGA GGATGACAAG 
AGGCGAGCGA AGGCGGAGAG CGCAGCCGCC GTGGCTGCCG TGGCGGACAA GAAAAGTTGC 
CCGCCAGACC TCTGGCATCG TTCACGCTAC TGCTGCTTCT GCCGCGGTGG CGGTAAGGGC 
CAGAGCGACC ACTCCTCCAG CGACGAGTTC GACTGGGGCG ATGATGACGA GGACAGCCAT 
CGCAACGCCG GCTACTTGCG CGACACCTCC GGCGTGCACC CGCCGGAGGT GTCCCTGTAC 
TCAGCGCTGG ACCGAAATAT CAGCCCGATG CAGCATCAGT CAGCGTCCTT GGCCGCCGTC 
TCGGAGCAGC TTCCGAGTTG CTCGAGCCCA ACGAGGACGG GCTACACGGG GGGCTGCAAT 
CGCAATAATA CGCGCACCTC GGGAAGCTGC ACACCCTCCA TGTCCTCGTT GTGGCTCGCG 
AGACCGGCGA CGTCTGCCGC TTCCAAAGCC GGCGACCGTG CATCACAGGA GGACGTGGTC 
GGCAGCGGAG AGCCAATGCA GTCGACAGCA GAGCATGCGA TGACAGCGAG CTCGGCGGAC 
GTGGAACACT CTCTACCGCG TGTCCCGGCC GCGGCAGCTG CAACGACAAC GGTGGCGCTG 
ACGAGAGCGA AGGATGCTTT GCCGGGTGAT ATTATCTGGA ACTGCGAGGT TGCTTTCTCG 
ACTGCGGCTG TGAGTCTCCT GCAGACGGAG GCAATGATGC GCAAGCGGTT CTGCAACATG 
AAGCGCGATC GCAAGGCGCT CTGCTTCCAG ATGGTCTGCC CCGCCGCATG CATCCTGCTA 
GCGATGCTGC TGAACGTGGC GGGAACGTAT ACCGTCAGAG AGCTGAGGCT AAACACGAGC 
AACTACCCAT ACGACACGCT GTGGGACACG ACGGGCTGTG CGGGCTACTT CAACGGAAGC 
TTCAGCGACG TGGCCGCGTC GCTTGCACGA AACCAGCGCA CGCGCGACTT GCGCATGACA 
AGTCTGTCGG ACTTCTACTA CTTCCTCCAA GACGAGTGGT TTGCGCACGG CAAAGTCGGC 
AAGTACAGCG GCCTCGCGTG CGGCGACCCT GTGGTGGCGA TGCTGCCTTC TATCGATCTT 
AACCCCGTCG TGTTGCTGAC GAACTACTCC GCCTATCACG AGTTTCCTAT AGCCATGTCG 
AACTTCTACA ATCTGCTCTT GAAGGAGGCT CGCGGCCCAT CCGTGTCCAT CACGGCGAGC 
GCCGGCACGC TGCCGTCGAG CTTCGCGCTT GCCTCCGAGA GAAGCATCAA GCTGCTGCTG 
ACGGCCATCA TCATTCTAGT GCCCTTCACC TTTCTCCCCG CGAACTGCGT GGCCTGGGTG 
GTGAAGGAGC GGGAGTGCCG GTCGCGCCAC CTGCAGGATA TCTGCGGCCT GCGCTACCTC 
GTCTACTGGT TCAGCAACTT TGTTTTCGAC TTCACGGCGT ACGCCGTGAC GATGCTGCTC 
GTTGTGACGA TTTTAGCTGT CTTCCGACGC GAGGAGTACA TCGGCGTCGA CACTGCTGGC 
GCGACTTTCA CGCTGCTTTC GGTCTTCGGC TTCTGCAGCA CGACCACGGC GTACTTTCTG 
CAGTTCTTCT TCGCAACCCA CTCAAGCGCG CAGAGCATCG TGATGGCGAC CGGCTTCATT 
ACCGGCTTCC TTCTGGTCAT CATTGTATTC GTGCTTCGAC TCTTGCCGTC GACAGAGGCG 
ACGTCGCACA GGTTGGGCTG GGCGTTCCGC ATCTTTCCCA CCTACGCCGT CAGCGAAGGC 
ATCGTGAATC TCGCCCTGCT CTCGCACTTC CAATCGTCGG ACGAGAGCTT GACAGCCTTC 
TCCATGCAGA CTATAGGCTG GCCGTGCGTG TACATGGCGG TGGAGGGGCC GCTGTTTCTC 
ATTCTTACGC TGCTGATCGA CCACCCACAC TGGCGAATGA GGCTGCTGCT GCGTGGCGGC 
GACGCGCACC GTCGGCGTCA TGGCAGCCAT CAGCACGGAG AGCAACCCGA CGCGAGTGAG 
AGTACCGATG ACGGTGTCGA CGAGGATAGC GACGTCGAGG ACGAGCGGGT CGAGGTGCAG 
CGGCGCATAG AGGCATACCG CGAAGACCTG AAGCGGCAGG AGAACATGTA CAAGGTGAGC 
GATGCTGCCG ACTCCGCATC CGTTGAGATG GCGGCGACGA GCACAGCTGG GGGCACCGGC 
GGTGACTTCT CGAGCTTCGG CGCTAAGCTG CCTATCATCG ACGCCGTCGC GGTCGTCGGC 
TTGCGCAAGC AGTATGATAA CGGCAAGGTG GCGGTGCACG ATCTCTCCTT CGGCGTCGTG 
CCGGGCGAGG TCTTTGGCCT TCTCGGCACG AATGGCGCTG GCAAGACAAC AACGATGTCG 
ATCCTGTGCC AGGAGTTCTA TCCCACCACT GGGCATGTCT ACGTGTGCGG GTACGACATT 
GTGGAGGAAA GTCGCGACGC GCTCCGGTGC ATCGGGTATT GCCCCCAGTT CGACGCAACG 
CTGGACCTGC TGACGGTGGA GGAGCACCTG AGTGTCTTTG CCGGCATCCG CGGCATCGTG 
CGGGAGCAGC AGAAGGACGT CGTGCGCGCG CTGCTGCAGC TGACTGGTCT GCGCGAATAC 
CGTCACACAA CGTCGGCAGC GTTGTCGGGC GGCAACCGCC GGAAGCTGAG CGTCGCGCTG 
TCGCTGATTG GTGGGCCGCC GGTGGTCATT TTCGACGAGC CGTCGGCGGG CATGGATCCC 
GTCGCTCGGC GTGAGATATG GACGTCGATG CAGGCCATTC GACACCGCTG CTCAATCATT 
TTGTGCACGC ACCACCTCGA GGAGGTGGAA GCCCTTGCCG ACTGTGTGGC CATCATGGTG 
GACGGGCGAC TGCGCTGCAT CGGCAGCAAG GTGCATCTGA GACAGAAGTA CGGCAGCGGC 
TTTGAGATGA CCATCCGCGT GCAGCCGCCG ACCACCGTCG ACGTGAGCTC CTCCACCGCG 
GCGGATGCGA GGCAGCGCAA GCGAGCTGCC GAGGCGGCGC TGGAGGCGAT CAAAGCGCGG 
CTCATTGTTT TCGTCGCTTC CTCGTTTCCG TCCTCACAGG TCAGCGAGGT GCGTGGCAAG 
CGACTTGTCT TTACACTGCC CAAGAACACG AACCTGCCGA GCGTCTTCGA GTGTTTGCAG 
GCAAACCGCG ACGCACTCTG CATTTCTGAC TACACGGTGT CGCAGACAAG TATTGAGCAG 
ATATTCATGC GCGTCAGCGG CGAGGTGGAG AAGGCGGAGA AGGTTCGCAC CGCCTTGTCA 
GAGCGGCGGC GTGATGTCGA GAACGCCTTG CGACGGAAGC ATGAGCAGCT GCACCTCAAG 
AGAGAAGAGT CTTCCGAGGG GCAACGTGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003721636.1
CDS	1..7173
Translation	MSPPSAVASLSSGGQLPSAAPAAERPQEQSRAPPAATALISTSQAAHDPGAGAPGLSHFDVATPLLLEDRRFARRDAGICGTDSYTMHSDSTHSSNLTMSSAYADAEGHRNAGNAAVNRGGSSSRSMESDVGDFASEYYGSMPTSGLGHDGHGRADDVHTGNLRAPFDRSSALDYWDTVTTTSFSESHTLTTKHGGGADDLASPTAGGAGWTHKDSSTWLDQLGATISRDMIERRRRWGSVVLEIAIPLVCTVCVVALWAAFGVTSSPDYSSLASIGDKGYNFAAGRYLAELCYNETWFGERNYIDGLRPCSSLDAASLRSVICNTVGEEELPVKGLCYRQGGRGLADFLSGMRNGLARVMPLDDIIARQWMAKKLSLTDSAAMALLIGAGFFSHTRINAIASSGKLYFAPSENVPLDMLQYLSNNSRFFQYVYNDTFDTVEEAHRVIKSGERGPTWALVNIRQLDENGLDLSIEMASTALPALTGMVDKAYSGGGGYDRSNMYYTSGYPSLMDALVKYYLMSLYAAGATRAETGSVQALAERRAEQQQRQQAQAAADDSVDVVLDTHKLLNAVRAAREPVGSGTQFFTDVAELGNSSAAVTSQKASRILATSAALRAKTSFTIFLGSMPWVPFKTSQVLTSANTIIGFVIVMAFLYPVSQLTRRLVLEKERRVREATLIMGLRPVYFWCSWFLYSVALMLTISLFMTLLMCTTFLTKSDASCVLLVLVMFSLTCVPLSGLMATLYNTSHMAVLMTPLLYFAMSLLVFAIYSASPAVYLGLSIFSPTCFAIILQIMLARESGDGFATRIFLDPNDNPNTATLLGFLVLDFGVYLLLMFYLDAVLPKAVGVSKHPLYFILEPARHCRRRRAERAWQAEQRRRQAASATDGGSGAREAPQPPPTQRRRTHDPKEGTHGGSDGSCCTGYNVATDDADEDLCDGEDARDPNGVYEAETFTPEQLSVRIIGLRKCFEHGGRRFVAVRNFCWQLPNAGISVLLGHNGAGKSTLINMMTGMLYPDGGDCYIGGHSIRSDLARARHQIGFCPQHNILWPELTCREHLEFFARLKGLRGAALEDAVMYTLKEVDLVARQSDTTRVLSGGMKRKLSVAIAFVGGSSLVFLDEPTAGMDVAARRHTWRLLLRMSRSRSVLLTTHFMDEADLLGDRVAIMSRGQLKCAGSSLFLKSRLGIGYNITISVDELLRVRDLDVFLQQHVPQAERLTSSGGEVGYRLPAKHVERFPGLLSALDRVGPLIGIRGYAISPTTLEEVFLTIARDAAEDDKRRAKAESAAAVAAVADKKSCPPDLWHRSRYCCFCRGGGKGQSDHSSSDEFDWGDDDEDSHRNAGYLRDTSGVHPPEVSLYSALDRNISPMQHQSASLAAVSEQLPSCSSPTRTGYTGGCNRNNTRTSGSCTPSMSSLWLARPATSAASKAGDRASQEDVVGSGEPMQSTAEHAMTASSADVEHSLPRVPAAAAATTTVALTRAKDALPGDIIWNCEVAFSTAAVSLLQTEAMMRKRFCNMKRDRKALCFQMVCPAACILLAMLLNVAGTYTVRELRLNTSNYPYDTLWDTTGCAGYFNGSFSDVAASLARNQRTRDLRMTSLSDFYYFLQDEWFAHGKVGKYSGLACGDPVVAMLPSIDLNPVVLLTNYSAYHEFPIAMSNFYNLLLKEARGPSVSITASAGTLPSSFALASERSIKLLLTAIIILVPFTFLPANCVAWVVKERECRSRHLQDICGLRYLVYWFSNFVFDFTAYAVTMLLVVTILAVFRREEYIGVDTAGATFTLLSVFGFCSTTTAYFLQFFFATHSSAQSIVMATGFITGFLLVIIVFVLRLLPSTEATSHRLGWAFRIFPTYAVSEGIVNLALLSHFQSSDESLTAFSMQTIGWPCVYMAVEGPLFLILTLLIDHPHWRMRLLLRGGDAHRRRHGSHQHGEQPDASESTDDGVDEDSDVEDERVEVQRRIEAYREDLKRQENMYKVSDAADSASVEMAATSTAGGTGGDFSSFGAKLPIIDAVAVVGLRKQYDNGKVAVHDLSFGVVPGEVFGLLGTNGAGKTTTMSILCQEFYPTTGHVYVCGYDIVEESRDALRCIGYCPQFDATLDLLTVEEHLSVFAGIRGIVREQQKDVVRALLQLTGLREYRHTTSAALSGGNRRKLSVALSLIGGPPVVIFDEPSAGMDPVARREIWTSMQAIRHRCSIILCTHHLEEVEALADCVAIMVDGRLRCIGSKVHLRQKYGSGFEMTIRVQPPTTVDVSSSTAADARQRKRAAEAALEAIKARLIVFVASSFPSSQVSEVRGKRLVFTLPKNTNLPSVFECLQANRDALCISDYTVSQTSIEQIFMRVSGEVEKAEKVRTALSERRRDVENALRRKHEQLHLKREESSEGQRA

Target ORF information:

RefSeq Version	XM_003721588.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin putative ATP-binding cassette subfamily A,member 1 (ABCA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003721588.1

ORF Insert Sequence:

ATGTCGCCGC CGTCAGCCGT CGCCTCTCTT AGCTCAGGTG GACAGCTGCC CTCTGCAGCG 
CCTGCGGCGG AGCGTCCGCA AGAGCAGTCA CGGGCACCCC CAGCGGCGAC GGCGCTCATC 
TCGACGAGCC AAGCGGCGCA CGACCCCGGC GCGGGCGCAC CTGGCTTATC GCATTTCGAT 
GTCGCTACCC CCCTCCTCCT TGAAGACCGA CGCTTCGCGC GGCGGGACGC CGGCATCTGC 
GGCACCGACA GCTACACGAT GCACAGCGAT AGCACGCATA GCAGCAACCT TACTATGAGC 
TCCGCCTACG CCGATGCCGA GGGGCACAGG AATGCCGGCA ACGCCGCCGT GAACAGAGGC 
GGCAGCAGTA GCAGGTCTAT GGAGAGTGAT GTAGGCGACT TCGCCAGCGA GTATTACGGT 
AGCATGCCCA CCTCCGGCCT CGGACACGAC GGGCACGGCC GCGCCGACGA CGTCCACACG 
GGCAACCTGA GAGCCCCCTT CGACCGCAGC AGCGCACTAG ACTACTGGGA TACCGTGACC 
ACGACTTCGT TCTCGGAGTC CCATACACTG ACGACTAAGC ACGGCGGCGG CGCCGACGAC 
CTTGCAAGCC CGACGGCTGG CGGTGCCGGA TGGACTCATA AGGACTCCAG CACGTGGCTC 
GACCAACTCG GGGCGACTAT TTCTCGCGAT ATGATCGAGC GGCGCCGCCG CTGGGGCAGC 
GTGGTGCTGG AGATTGCGAT CCCGCTTGTG TGCACGGTCT GTGTGGTGGC GCTGTGGGCC 
GCCTTCGGCG TTACTTCCTC GCCGGACTAC AGCTCGCTGG CCTCTATTGG TGATAAAGGA 
TACAACTTCG CGGCTGGCAG GTACCTGGCG GAACTCTGCT ACAACGAGAC GTGGTTTGGG 
GAGCGGAACT ACATCGACGG ACTGCGCCCC TGCAGCAGCC TAGACGCTGC GTCACTGAGA 
TCAGTGATCT GCAACACGGT CGGCGAGGAG GAGCTGCCCG TGAAGGGGCT GTGCTACAGA 
CAAGGGGGCC GGGGGCTTGC TGACTTTCTC AGCGGTATGC GCAACGGCCT CGCGCGGGTG 
ATGCCGCTAG ACGACATCAT CGCCCGCCAG TGGATGGCCA AAAAGTTGTC CTTAACGGAT 
TCGGCGGCGA TGGCGCTGCT GATCGGCGCG GGGTTCTTCT CCCACACGCG CATCAACGCT 
ATCGCGTCGT CCGGCAAGCT GTACTTCGCC CCAAGCGAAA ACGTGCCACT CGACATGCTC 
CAGTACCTGT CCAACAACTC TAGGTTTTTC CAGTACGTGT ACAACGATAC CTTCGACACC 
GTCGAGGAGG CGCACCGCGT GATCAAGAGC GGCGAGCGTG GACCCACATG GGCTCTCGTG 
AACATCCGCC AGCTGGACGA GAACGGCTTG GACTTGTCTA TCGAAATGGC CTCGACAGCG 
CTGCCGGCGT TGACGGGGAT GGTGGACAAG GCGTACTCCG GCGGCGGGGG CTACGATCGA 
TCCAACATGT ATTACACGAG CGGCTACCCG TCCTTGATGG ACGCACTTGT GAAGTACTAC 
CTCATGTCCC TGTACGCGGC CGGCGCAACG CGCGCTGAAA CGGGCAGCGT TCAGGCCCTG 
GCCGAGAGGC GGGCGGAGCA ACAGCAGCGG CAACAGGCGC AGGCAGCGGC TGACGACTCG 
GTGGACGTGG TTCTGGATAC GCACAAACTG CTCAACGCTG TGCGGGCGGC GCGGGAACCA 
GTGGGCAGCG GTACCCAGTT CTTCACCGAT GTCGCCGAGC TGGGCAACTC CTCGGCGGCC 
GTGACGTCAC AGAAAGCCTC TCGCATCCTG GCGACGAGCG CGGCCCTTCG CGCGAAGACG 
AGCTTCACGA TCTTCCTGGG CAGCATGCCA TGGGTGCCCT TCAAGACGAG CCAGGTGCTG 
ACTTCTGCGA ACACCATCAT CGGCTTCGTC ATTGTGATGG CGTTTCTCTA CCCTGTCTCG 
CAGTTGACGC GGCGGCTCGT GCTAGAGAAG GAGCGGCGCG TGCGGGAGGC GACGCTGATC 
ATGGGGCTCA GGCCGGTATA TTTTTGGTGC AGCTGGTTCC TTTACAGCGT CGCCCTCATG 
CTCACGATCT CCCTCTTCAT GACGCTGCTG ATGTGCACAA CGTTCCTGAC GAAGAGCGAT 
GCCTCATGCG TGCTTCTTGT CCTTGTCATG TTCTCCCTCA CGTGCGTGCC GCTGTCGGGG 
CTGATGGCCA CTCTCTACAA CACGTCGCAC ATGGCGGTTC TCATGACGCC ACTCCTCTAC 
TTTGCCATGT CTCTGCTGGT CTTCGCGATC TATTCGGCCA GTCCGGCCGT CTACCTCGGC 
CTCAGCATCT TCTCGCCCAC CTGCTTCGCG ATCATCCTGC AGATCATGCT CGCCCGGGAG 
AGCGGCGACG GCTTTGCGAC GAGGATCTTT CTGGACCCGA ACGACAACCC CAACACCGCC 
ACGCTCTTGG GATTCCTCGT CCTGGACTTT GGTGTGTATC TGCTGCTCAT GTTCTACCTC 
GATGCGGTGC TGCCCAAGGC GGTTGGTGTG TCGAAGCACC CGCTCTACTT CATCCTAGAA 
CCGGCCCGGC ATTGCCGTCG CAGGCGTGCG GAAAGGGCGT GGCAGGCGGA ACAGCGGCGG 
CGTCAAGCGG CGTCTGCCAC CGACGGCGGC AGTGGTGCAA GGGAGGCCCC GCAGCCGCCA 
CCGACGCAGA GGAGGAGGAC TCATGATCCC AAAGAGGGGA CGCACGGCGG CAGCGATGGC 
TCGTGCTGCA CTGGGTACAA CGTTGCAACG GATGACGCCG ACGAGGACCT GTGCGACGGC 
GAGGACGCCC GTGATCCGAA CGGCGTGTAC GAGGCGGAGA CCTTCACGCC GGAACAACTG 
TCCGTGCGCA TTATCGGCCT CCGCAAGTGC TTCGAGCACG GTGGCCGCCG CTTCGTTGCG 
GTGCGGAACT TCTGCTGGCA GCTGCCGAAC GCTGGCATCT CCGTGCTGCT GGGGCACAAC 
GGCGCCGGCA AGTCGACGCT GATCAACATG ATGACGGGCA TGCTCTACCC GGATGGCGGG 
GACTGCTACA TCGGCGGCCA CTCCATCCGC TCCGATCTGG CGCGCGCCCG CCACCAGATC 
GGTTTCTGTC CACAGCACAA CATTCTCTGG CCGGAGCTGA CGTGCCGTGA GCACCTCGAG 
TTCTTTGCGC GGCTGAAGGG GCTGCGCGGG GCTGCGCTGG AGGATGCTGT GATGTACACC 
CTCAAGGAGG TGGATCTTGT GGCGAGGCAG AGCGACACGA CGCGCGTTCT CTCGGGTGGC 
ATGAAGCGGA AGCTCTCGGT CGCCATCGCG TTTGTAGGTG GCAGCAGCCT CGTCTTCCTC 
GATGAGCCGA CCGCCGGCAT GGACGTGGCG GCGCGGCGGC ACACGTGGAG GCTGTTGCTG 
CGCATGTCGC GCAGCCGGAG CGTACTGCTG ACCACGCACT TTATGGACGA GGCGGACTTG 
CTCGGTGACC GGGTGGCCAT CATGAGCCGT GGCCAGCTTA AGTGCGCTGG CAGCTCGCTC 
TTCTTGAAGT CGCGCCTGGG TATCGGGTAC AACATCACCA TCTCTGTCGA CGAGCTGCTA 
CGCGTCCGTG ACCTCGATGT CTTTTTGCAG CAGCACGTGC CGCAGGCGGA GCGCTTGACA 
AGCAGTGGCG GCGAGGTCGG CTACCGCCTG CCTGCGAAGC ATGTGGAGCG CTTCCCAGGA 
CTGCTGAGCG CGCTCGACAG AGTTGGGCCG CTGATCGGCA TTCGTGGCTA CGCCATCTCT 
CCCACAACGC TGGAGGAGGT GTTTCTGACG ATCGCCCGCG ACGCCGCGGA GGATGACAAG 
AGGCGAGCGA AGGCGGAGAG CGCAGCCGCC GTGGCTGCCG TGGCGGACAA GAAAAGTTGC 
CCGCCAGACC TCTGGCATCG TTCACGCTAC TGCTGCTTCT GCCGCGGTGG CGGTAAGGGC 
CAGAGCGACC ACTCCTCCAG CGACGAGTTC GACTGGGGCG ATGATGACGA GGACAGCCAT 
CGCAACGCCG GCTACTTGCG CGACACCTCC GGCGTGCACC CGCCGGAGGT GTCCCTGTAC 
TCAGCGCTGG ACCGAAATAT CAGCCCGATG CAGCATCAGT CAGCGTCCTT GGCCGCCGTC 
TCGGAGCAGC TTCCGAGTTG CTCGAGCCCA ACGAGGACGG GCTACACGGG GGGCTGCAAT 
CGCAATAATA CGCGCACCTC GGGAAGCTGC ACACCCTCCA TGTCCTCGTT GTGGCTCGCG 
AGACCGGCGA CGTCTGCCGC TTCCAAAGCC GGCGACCGTG CATCACAGGA GGACGTGGTC 
GGCAGCGGAG AGCCAATGCA GTCGACAGCA GAGCATGCGA TGACAGCGAG CTCGGCGGAC 
GTGGAACACT CTCTACCGCG TGTCCCGGCC GCGGCAGCTG CAACGACAAC GGTGGCGCTG 
ACGAGAGCGA AGGATGCTTT GCCGGGTGAT ATTATCTGGA ACTGCGAGGT TGCTTTCTCG 
ACTGCGGCTG TGAGTCTCCT GCAGACGGAG GCAATGATGC GCAAGCGGTT CTGCAACATG 
AAGCGCGATC GCAAGGCGCT CTGCTTCCAG ATGGTCTGCC CCGCCGCATG CATCCTGCTA 
GCGATGCTGC TGAACGTGGC GGGAACGTAT ACCGTCAGAG AGCTGAGGCT AAACACGAGC 
AACTACCCAT ACGACACGCT GTGGGACACG ACGGGCTGTG CGGGCTACTT CAACGGAAGC 
TTCAGCGACG TGGCCGCGTC GCTTGCACGA AACCAGCGCA CGCGCGACTT GCGCATGACA 
AGTCTGTCGG ACTTCTACTA CTTCCTCCAA GACGAGTGGT TTGCGCACGG CAAAGTCGGC 
AAGTACAGCG GCCTCGCGTG CGGCGACCCT GTGGTGGCGA TGCTGCCTTC TATCGATCTT 
AACCCCGTCG TGTTGCTGAC GAACTACTCC GCCTATCACG AGTTTCCTAT AGCCATGTCG 
AACTTCTACA ATCTGCTCTT GAAGGAGGCT CGCGGCCCAT CCGTGTCCAT CACGGCGAGC 
GCCGGCACGC TGCCGTCGAG CTTCGCGCTT GCCTCCGAGA GAAGCATCAA GCTGCTGCTG 
ACGGCCATCA TCATTCTAGT GCCCTTCACC TTTCTCCCCG CGAACTGCGT GGCCTGGGTG 
GTGAAGGAGC GGGAGTGCCG GTCGCGCCAC CTGCAGGATA TCTGCGGCCT GCGCTACCTC 
GTCTACTGGT TCAGCAACTT TGTTTTCGAC TTCACGGCGT ACGCCGTGAC GATGCTGCTC 
GTTGTGACGA TTTTAGCTGT CTTCCGACGC GAGGAGTACA TCGGCGTCGA CACTGCTGGC 
GCGACTTTCA CGCTGCTTTC GGTCTTCGGC TTCTGCAGCA CGACCACGGC GTACTTTCTG 
CAGTTCTTCT TCGCAACCCA CTCAAGCGCG CAGAGCATCG TGATGGCGAC CGGCTTCATT 
ACCGGCTTCC TTCTGGTCAT CATTGTATTC GTGCTTCGAC TCTTGCCGTC GACAGAGGCG 
ACGTCGCACA GGTTGGGCTG GGCGTTCCGC ATCTTTCCCA CCTACGCCGT CAGCGAAGGC 
ATCGTGAATC TCGCCCTGCT CTCGCACTTC CAATCGTCGG ACGAGAGCTT GACAGCCTTC 
TCCATGCAGA CTATAGGCTG GCCGTGCGTG TACATGGCGG TGGAGGGGCC GCTGTTTCTC 
ATTCTTACGC TGCTGATCGA CCACCCACAC TGGCGAATGA GGCTGCTGCT GCGTGGCGGC 
GACGCGCACC GTCGGCGTCA TGGCAGCCAT CAGCACGGAG AGCAACCCGA CGCGAGTGAG 
AGTACCGATG ACGGTGTCGA CGAGGATAGC GACGTCGAGG ACGAGCGGGT CGAGGTGCAG 
CGGCGCATAG AGGCATACCG CGAAGACCTG AAGCGGCAGG AGAACATGTA CAAGGTGAGC 
GATGCTGCCG ACTCCGCATC CGTTGAGATG GCGGCGACGA GCACAGCTGG GGGCACCGGC 
GGTGACTTCT CGAGCTTCGG CGCTAAGCTG CCTATCATCG ACGCCGTCGC GGTCGTCGGC 
TTGCGCAAGC AGTATGATAA CGGCAAGGTG GCGGTGCACG ATCTCTCCTT CGGCGTCGTG 
CCGGGCGAGG TCTTTGGCCT TCTCGGCACG AATGGCGCTG GCAAGACAAC AACGATGTCG 
ATCCTGTGCC AGGAGTTCTA TCCCACCACT GGGCATGTCT ACGTGTGCGG GTACGACATT 
GTGGAGGAAA GTCGCGACGC GCTCCGGTGC ATCGGGTATT GCCCCCAGTT CGACGCAACG 
CTGGACCTGC TGACGGTGGA GGAGCACCTG AGTGTCTTTG CCGGCATCCG CGGCATCGTG 
CGGGAGCAGC AGAAGGACGT CGTGCGCGCG CTGCTGCAGC TGACTGGTCT GCGCGAATAC 
CGTCACACAA CGTCGGCAGC GTTGTCGGGC GGCAACCGCC GGAAGCTGAG CGTCGCGCTG 
TCGCTGATTG GTGGGCCGCC GGTGGTCATT TTCGACGAGC CGTCGGCGGG CATGGATCCC 
GTCGCTCGGC GTGAGATATG GACGTCGATG CAGGCCATTC GACACCGCTG CTCAATCATT 
TTGTGCACGC ACCACCTCGA GGAGGTGGAA GCCCTTGCCG ACTGTGTGGC CATCATGGTG 
GACGGGCGAC TGCGCTGCAT CGGCAGCAAG GTGCATCTGA GACAGAAGTA CGGCAGCGGC 
TTTGAGATGA CCATCCGCGT GCAGCCGCCG ACCACCGTCG ACGTGAGCTC CTCCACCGCG 
GCGGATGCGA GGCAGCGCAA GCGAGCTGCC GAGGCGGCGC TGGAGGCGAT CAAAGCGCGG 
CTCATTGTTT TCGTCGCTTC CTCGTTTCCG TCCTCACAGG TCAGCGAGGT GCGTGGCAAG 
CGACTTGTCT TTACACTGCC CAAGAACACG AACCTGCCGA GCGTCTTCGA GTGTTTGCAG 
GCAAACCGCG ACGCACTCTG CATTTCTGAC TACACGGTGT CGCAGACAAG TATTGAGCAG 
ATATTCATGC GCGTCAGCGG CGAGGTGGAG AAGGCGGAGA AGGTTCGCAC CGCCTTGTCA 
GAGCGGCGGC GTGATGTCGA GAACGCCTTG CGACGGAAGC ATGAGCAGCT GCACCTCAAG 
AGAGAAGAGT CTTCCGAGGG GCAACGTGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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ABCA1 cDNA ORF clone, Leishmania major strain Friedlin

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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