LINJ_35_4000 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_35_4000
Entrez Gene ID	5073297
Full Name	U5 snRNA-associated splicing factor
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001469165.1	XP_001469202.1	U5 snRNA-associated splicing factor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_35_4000 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_35_4000 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc06509	XM_001469165.1 Latest version!	Leishmania infantum JPCM5 U5 snRNA-associated splicing factor partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_35_4000 ( XM_001469165.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001469202.1 Leishmania infantum JPCM5 U5 snRNA-associated splicing factor partial mRNA.

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CloneID	OLc06509
Clone ID Related Accession (Same CDS sequence)	XM_001469165.1
Accession Version	XM_001469165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	U5 snRNA-associated splicing factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009419). COMPLETENESS: incomplete on both ends.

ATGAGCGTGT CGGTCCCTCC TGGGTTTGAG GACGATGACG ACCTGGCCAC TGTCGACCCT 
GCGCTACTTG CGGAGATGGA GGAGGAGGAG CAGAAGGAGT CGAGGAAGCA GCAGATCCTT 
ACATGGCAGC GCATGAACAC GGAGCGATAC GGCTTCCGCG CGGCGTACCG GGAGGCAGTG 
GGGGAGAAAG ACCTGATGCC GCCAGAGTTC ATCCGCAAGG TGGTGAAGGA CAATGGTGAC 
CTCGGCGGAA AGCGCTTCAA ATCGGAGCGC AAGCTGTGCG TGGGTATGCT GCCCTACATT 
CCACTGGCTC TGCTCAAGCT CCTAGAAAAC ATGCCAATGC CCTGGGAGGA GGCGCGTTAC 
GTGACCGTAG TGTACCACGT CGGAGGCGTG CTCACCATCG TGGACGACAC CCCCACAGTG 
GCGGAGCCGC TGTACGTGTC ACAGTGGGGT TCGATGTGGA CGAAGATGCG CGCCAACAAG 
GTAGAGCTGC TTCAAGAAGG TGGCGGCTTC AGGCGCGCGG TGCACAAGGG AGACGAAAAC 
GAGCCGCCGC TGGACTTTGC CGACTACCTC ATGGACCGCG TGCCGCCTCC ACCGGTGCTC 
GACGATCTCG ATGAGGAGGA CAACGCCAGC ATCGTGGGCT GGTTCTATGA CCCTTTTCCG 
CGCCTGGTGG CTCCCAACCA GATTCGCGGC CCTCGGCGGC CCAATGGGTA CTACTTCAAC 
CTTGATGTGA TGGAGGCGCT ATACCGCAAC GCGTCACCCA TTCTGCCGAC GCTTGACGAC 
CGCAACTACT TTTACCTGTG GGACCTCAAG TCCTTCTACG CTGCCAAAGC GATGCACCTG 
TCCATTCCAC GCGGCCCGAA GTTCGAGGCC CCACCTGCCA TTCGCGTGAA GCAGGACGAG 
GACGACGACT GGACGGAGTT CAACGACCTG CGTCGCATCA TCCACCGCGA CAATCCGCGC 
AAGCCGCGCT TCACGATGCT GACGGAGCGA CAGATTGCCT TCCCTTTCCT GTATGGCTCA 
GTGGTGGACG GGGTCGAACT GGCCCCGTAT CACTACCCAG CCACCGTGCG CGTCGAGAAC 
GACGACCCCG AGCTACCGTG CTTTACTTTC CACCCATCGC TGAACGCAAT CAAAGCAGTG 
GAGAAGAATT ACACAGACGT GCCGCGCGCG CAGGCCGCGT TGTGCTCCTC TACCTGGCAT 
CGCGCGGGCG GCCAGCATGT TGAGACCGCC GCGGACGTGC CGTTAGAGCT GCCGGACGAC 
TTTGAGCCGT TTCTGTCATC GCTCCCGCTG GAGCAGCCCA ACACGAAGAC GGGCCTGTCG 
CTGCTTTTTG CTCCGGCGCC CTACAACTCT TTTGAGGGAG GCATGAAGCG GCGCATCGAT 
GTGCCGCTGT GCGACAAGTG GTACCAGGAC CCACCGGACC TGCTCACGAC GGACGACCGA 
GACAAGATTC TGCGCAGCTA CACCCAGCTA CTCAAGCACC ACGTGAAGCG CTCCCTGCAG 
CACACCGTGC GCCAACGGAG CAGTTCCGCG ACCGCCGCCA CAGCAGCTGA AGGGGCGGGG 
ACGATGGACA TGAGCGCGAT GCAGGAGAAG CGCCGGCTGG AGAAAATGGC AGGGATGCGG 
TACTTTTCCA AGACGCGCAT GGAGTGGCTA GAAGCGGGGC TTCAGGTGAT GCGGCAGGGC 
CACAACATGC TTGTTCAGCT CATTAACATG AAGTGCCTGC CGTATGTGCA TATCGACTAC 
AACTTCGAGG CGAAGCCGAC CCGCACGCTG ACGACGAAGG AGATCAAGAA GAGCCGGCTC 
GGCCCCGCGT TTCACATGAT CCGTGAGCTG CTAGGCTTCA TGAAGCGGCT CATCGACATG 
CATGTGATGT ATCGGTTAGG GTCGACTGAC GCGCTGCAGT TGGCGGATGC TACCCAGTAC 
CTCTTCTCTC ACGTCGGCGT CCTGACGGGG GTGTACCGCT ACAAGTTGCG GGCTATGCGG 
CAGATCAAGA GGAGTCGTGA CCTTAAGCAC ATGCTGTACA GCAAGTTCAA CGTTGGCGGC 
GTGCCAAGTG GGCCTGGAAA CGGTTTTTGG GGCCCGGCGT GGCGTGTGTG GGTATTCTTT 
ATGCGCGGCA TGACACCGCT TCTTCAGCGC TATCTGAGCA ATCTGACCGA CCGCGTGCTG 
CACGGTCGTT TGCAGAAAGG CAAGATGTCG GGCAAGAAAA TCACGCGTCA GCGGGTCGAG 
CCGGACAAGG ACGTGAACAT CAAGGAGGCG TTCCGCCGTG AGCTGCGAGA GATGCTGCCG 
GACAACGTGA AGGCTTCGGT CATTGTGACG ATGGACCAGC ACATGAACGA GGCGTTCCGG 
CACTGGCGCG CCGGCCTGCC GTGGAGCGTG CCTGGGCTTG CGAAGCCGCT GACAGACCTC 
GTCGGTAAGT ACGTGAAGCT TCGCTCTGAG GAGTACATCC GCACGACGCA GCTGCAGCGG 
CGCCGCATTG CGGAGGGGGA TACTGTGGAC AAGCAGGCCT TCATGAAAAA CCTAGGGCGC 
TTGACGCGAC TGAAGCTCAT GGATGAGCAG CGCCGCCAGC GTCGATACAT GACAGGGGAG 
GACGCCGCCG CGATCATGGC GCCCGCTGAG GCGACGGAGA TTTATCGCAT GATGGCGAAC 
TGGCTCAACG ACCGCGGATT CAAGAAGATA TCCTTCCCAG ACCCGTCCAA GACGGCAGAG 
CTGTCGCTGC TGCAGCTTTC CCTCAATCGA CTGCGTGACC AGCACAACAT CGCGAACCGC 
CTCACGGCGA ACCAGCGTGA GGAGCAGGCC CGCATCGAGG AGTCCTTTAA CGCGCCTCAC 
GAGGCCCTGT CCCGCATCGT AGACGCCCTT GCACACCAGC GCCGCTTCAA AAACGTCGAG 
GTGGAGTACA TGGACAACTT TTCACACCTG TACCCGCTCT ACACTGTCGT GCCGCAGGAG 
AAGATGATTG ACTCCTTCCT CGATCAGTAC CTCTGGTACA AGGCGATGAA TGTGCAACGT 
CTCTTCCCCA ATTGGGTGAA ACCGTCCGAT GTGGAGCCGT TGCCACAGCT GGTGTACAAG 
TGGTGTGAGG GCATCAACAA CATCCCCGAT ATATGGGACG TATCGCACGA CGAGTCTGTG 
GTGCTGTTGC ACAGCAACCT GGAGGATGCA TTCTACGACA ACGTGGACTG GAACTTCTTC 
CGTCCGATGC TGGAGCTGAT CATGGATAAG TCGCTCGTCG ACTACATTGT GAGCCGGCAC 
GATGTCGTGG TGGAGTTCAA GGACATGAGC TACCATCACT ACAAGGGGCT TATCCGCGGC 
TTCATGTTTT CGTCATTCCT GGCGCAGTAC TGGGGGCTCG TGATGGATGT GCTGCTGCTC 
GGCACTCAGC GAAGCAAGGA GATTGCCGGT ACGGCGTTAA AGCCGAACCC ATTCATGTCT 
TTCTTCCGAG ACCCATACCT CGCCGCGTCG CATCCGATTC GCGCGTACTG CAGGTACAAG 
AACGAGGTCT ACATCGTGGT GCGCTACACC AAGACGGAGG CGGATGAGGT GCGGCGCCGC 
TACCTGGAGG AGACCAAGTC GGATCCGGAG ATGCGTGCGA TTAACGCATC CGTCTATGGA 
TTCAAGAACG CGAAGGAGTG GCCACGCGAC GCACGGATGC GCCTTTTCCT GTCCGACGTA 
AACTTGGCGC GCGCGGTGCT ATGGGAGTTC CGCAGCCGCC TGCCGCCGTC GATGGCGACC 
ATCAACGACG CAAACAGCTT CGTCAGCGTT TACTCCAAGG ACAACCCCAA CCTGCTCTTC 
GAAATGGCAG GCTTCTCCGT GCGGCTGCTG CCGGTGGTGC GGACCGAGGC GGACATTCTC 
GAGAGCGAGT CTATGTGGAG TCTGCGCAAT CACACAACCA AGGACATCTC CTGCCGAGCC 
TTCCTGCAAG TGACGAAGGA GCATATCAAC ATGATCCGCA ACAAGGCGCG CCGCACTGTA 
ATGATGGTCG GCAGCTCCAC CTTTCACAGC ATCGCGGCCA AGTGGAACGC GCTGATTACG 
GAGATTGTGC CATACTATCG CGAGGCGATT CTCGGGACGG AGGAGCTGCA GGCGATCCTG 
GCGCGCGCGG AGCACCGCAT GCAGGCGCGT GTGATGATGG CGCTGAACTC GCGCGCCAAG 
TCGCGCTTCC CACCGGCGAT GTTCTACGCC CCGTCCGATC TTGGCGGCCT CGGCATGCTC 
TCGGTCGGAC ACTCGCTGAT CCCTGCGAAG GACACCATCT ACTCGAAGAC GACCAGCACG 
GGCGTCCAGT TCTTCTACGC CGGCCTCACT AACGAGGAGG GCATCCCGAT CCCCAACGTG 
CTGCAGTATT ACACGCCGTG GGAAACGGAG ATCAAGGAGA GCGAGAAGGC ATGGCTGGAG 
TTCCGCACCC GCGAGCGTGA GGCCAAGGCG CGTGGGGGCC GCGTGTCGCT GGACGACATC 
GAGGACATCA TCGACAAGGG CATCCCGCGC ATCCGCGTGC GCTTCTCGCG ACACGCGCCG 
CTCTTCCACT TCGACACCGG CTTCCGGGCA CGCATGGAGT TCCAGCGATA CCTGGCCGGC 
AAGTACCTGA AGAACTGGTG GTTTCACATG GAGCACGACG GCAACATCTG CGGCGGCGTG 
ATGGAGCGCT ACCGCGCTGA TATGAACAAC GCCCTCGGTG GTGTGGAGGC GATTTTGGAG 
CACAGCCTCT TCAAGGGGAC GGGCTTTCCG TCGTGGGAAG GCATCGAGTT CGACCGCTCT 
GGCGGCTTTG AAAACTCGAA GAAGGATACA AAACTGGCGA AGCAGCAGCG TGCCGGTCTG 
AGCAAGGTGC CGAACCAGCG GTTCGCGCTT TGGTGGAGCC CTACGATCAA CCGCTCCGAC 
ATCCAGGCCG GCTTCGAGTC GAAGGTGGAG ACGACGGGTG TCTTCATGTG CGGTAAGCTG 
GAGACCATCA AGAAGTCGCT CATCAAGATC TTTAGCGGCT CTCTCTGGGA GAAGTCACAC 
GGCGCCGTCG TGAACGACGT GGCGAGCAAG CTGAAGGACA GCCTCATGGA CCTTGGCGCA 
GCCTCCATAA CGCTGCAACA GCAGCACCCA CAGAAGTCCT ACACCTTCAC GAGCTCGGCT 
GCCGATGTCA TATTGGTGAG CGCGGCGCGC TGGTCGGTAT CGTCGAAGCC GACCAGTCTC 
GCCGACGAGG TCGGCGACGT GTACCAACAC AGCACCACCT CGAAGTTCTG GATCGATGTG 
CAGCTGCGGT GGGGAAACTA CGACAGCCAC AACATTGCCG AGTACGCGAG GAAGAAGTTC 
TACGAGTACT CGACGGCGCG CATGTATCCG TTTCCTGCCG GCATTGTCGT TGCGATTGAC 
CTGGCCTACA ACTGCCACAG CGCGTTTGGC TACTGGATAC CTGGCATGAA GCCGTTCATG 
AGTAAGCTGA TGCCCGTCAT TATGAAGAAC AACATCACCC TCAACACGCT GCGCGACCGC 
ATGAAGCGCG ACCTGGGACT CTTCAGCTCT GCCCCGACGG AGGCGAGCCT GTCCGACACA 
AACATCGCTG AGCTGTTCTC CAGCGGGATG CGCACGTGGA TTGTAGACGA CAGCGCCACG 
TATGTGACGA GCGAGCAGCC GACCCCAGAC GGCGGCAAGA AGTTCAAGTC CGAGAATGGC 
GCGGTGCTCG TCTTTGAGCC CGTCTCCGGC TCTTTGAAGA TGAGCGTGGT GCACCGGAGC 
GTCTTTTCCG GGCAGAAGCG CCGCTCGAAG CTGGCGCGCG AGAAGGCAGC GGAGGAGATT 
GCGTCGTGGC TACGTAGCTG CCCGCCTAGC GAGCGTCCGA GCAAGATCAT CGTCACCCGC 
TCCCGCTTCC GCCAGACGCT GCATAACATG CTGGTGCTAG ACTACCCGAA CATCATCATT 
GGCCAAAGCG ACTTGAACCT GCCGCTGCCG ATGGTGTTGC GTCACAGCCG CCTCGTCGAG 
CTGCGCATCG CGGCGATGGA AAGCAAAGGG TGGGAGTTCT GCATCTACGA CGACTGGCAG 
CAGAGTCTGC AGTTCCAACC GATCACGTGC TTCAACCTAC TGAACCTGGT TCTGCGTGGC 
TACCACATAA ACCTGCAGCG CACACGGCAG ATTCTCGTGC CTGACCTGCA CGTCGAGGTA 
AGCGCGTCTC ACTTCTGGCC CACGTACGCG ACCCGACAGG AATGGGAGCA GGTGTCCATC 
CGTCTGCAGG AGATGATCAT CGCCGACGCC GCGCGCCGCA TGAACGTGAG CCCGAAGGAC 
TTCACGGAAA AGGAGAAGGA GGGCATTCTT CTGGGCAAGA GAATGGCCAA TGTGGAGATT 
CAGCAGGAGG AGATGAAGGA GATCGAGGCC ATGCGGCGCA CAAAACTGGC AGAGACGCAG 
ACGGTCAACG TTGTCACAAA CAGCGGCGAC GTCGTAAAGC GCAAGGTGAA GGCCGCCTTC 
GACTTTGGAG CGACGGCGTT GAACAACAAC TGGCGACCCC GCTCCTTGGC CGATGCCGCC 
TTTCTTGACG AGAACACTCC CATCACCTTC GACGCCGCCG GCGCCACCGG GGCAAGCGAC 
CAGCTCATCT TCTCTGAGGA CGCGATTCAG AAGCTGCTGG CGTGCTGCGA CGTTAAGGTT 
CAGTGCTGCG CGTACATGTT TGGCCATGCG CTGCCCGACT CGCCAAACAT CAAGGAGGTG 
CTGTGTGTCA TGATTCCGCC GCAGTTCGGG ACGGCTGTCG AGGCCCGCAC GCCACCACGC 
ATTCCGTTTG ACGCGGCAGC ACTGCAGGAG GCGAACCTGT CCTTCTTGGG ACTCATGAGG 
ATTGGCGAAA GCGAGGCGCA GCTGACCTCG CACGACCTCG CCCTCCAGGC GCGTGCGCTG 
ATCGCGAACG AGGGGATGGT GCCGCAAGGG TTTGTGACTG CCGTCCTGGA GATGTCGGAA 
GAGGGAGTGA TGGCGCGGTG CTACAGCACT ACCGCGGACG GCATTGCATG GGCGCAGAGT 
GAGCACGAGC GGCTGCTGAA GAGGACTCCG GAGGCGACGG ACCCATCCTT CTCCTCCCCG 
TGCCGCGGCA CCCTTTCCTC GGAGGCTCGT AGCTTCTTCC TGGTACCGGC GGATCGGGTC 
TGGAACTACT TCTTCAAGGG GGCACTGTGG CGCGAGAACA CCGAGTACGA CGTCGTCGTC 
GACGTGCCGT TGCCCTTCTT CCATGCGCTG CACCGCCCAG ACCACTTCCT TAACTTCACT 
CGCATCGGTG ACGGCGCCGA GGTGGTGGAT GAGACGGACC CAAACGACGT GTTCGGCAAC 
GAGGCCGGGC TGGCAGGTGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001469202.1
CDS	1..7284
Translation	MSVSVPPGFEDDDDLATVDPALLAEMEEEEQKESRKQQILTWQRMNTERYGFRAAYREAVGEKDLMPPEFIRKVVKDNGDLGGKRFKSERKLCVGMLPYIPLALLKLLENMPMPWEEARYVTVVYHVGGVLTIVDDTPTVAEPLYVSQWGSMWTKMRANKVELLQEGGGFRRAVHKGDENEPPLDFADYLMDRVPPPPVLDDLDEEDNASIVGWFYDPFPRLVAPNQIRGPRRPNGYYFNLDVMEALYRNASPILPTLDDRNYFYLWDLKSFYAAKAMHLSIPRGPKFEAPPAIRVKQDEDDDWTEFNDLRRIIHRDNPRKPRFTMLTERQIAFPFLYGSVVDGVELAPYHYPATVRVENDDPELPCFTFHPSLNAIKAVEKNYTDVPRAQAALCSSTWHRAGGQHVETAADVPLELPDDFEPFLSSLPLEQPNTKTGLSLLFAPAPYNSFEGGMKRRIDVPLCDKWYQDPPDLLTTDDRDKILRSYTQLLKHHVKRSLQHTVRQRSSSATAATAAEGAGTMDMSAMQEKRRLEKMAGMRYFSKTRMEWLEAGLQVMRQGHNMLVQLINMKCLPYVHIDYNFEAKPTRTLTTKEIKKSRLGPAFHMIRELLGFMKRLIDMHVMYRLGSTDALQLADATQYLFSHVGVLTGVYRYKLRAMRQIKRSRDLKHMLYSKFNVGGVPSGPGNGFWGPAWRVWVFFMRGMTPLLQRYLSNLTDRVLHGRLQKGKMSGKKITRQRVEPDKDVNIKEAFRRELREMLPDNVKASVIVTMDQHMNEAFRHWRAGLPWSVPGLAKPLTDLVGKYVKLRSEEYIRTTQLQRRRIAEGDTVDKQAFMKNLGRLTRLKLMDEQRRQRRYMTGEDAAAIMAPAEATEIYRMMANWLNDRGFKKISFPDPSKTAELSLLQLSLNRLRDQHNIANRLTANQREEQARIEESFNAPHEALSRIVDALAHQRRFKNVEVEYMDNFSHLYPLYTVVPQEKMIDSFLDQYLWYKAMNVQRLFPNWVKPSDVEPLPQLVYKWCEGINNIPDIWDVSHDESVVLLHSNLEDAFYDNVDWNFFRPMLELIMDKSLVDYIVSRHDVVVEFKDMSYHHYKGLIRGFMFSSFLAQYWGLVMDVLLLGTQRSKEIAGTALKPNPFMSFFRDPYLAASHPIRAYCRYKNEVYIVVRYTKTEADEVRRRYLEETKSDPEMRAINASVYGFKNAKEWPRDARMRLFLSDVNLARAVLWEFRSRLPPSMATINDANSFVSVYSKDNPNLLFEMAGFSVRLLPVVRTEADILESESMWSLRNHTTKDISCRAFLQVTKEHINMIRNKARRTVMMVGSSTFHSIAAKWNALITEIVPYYREAILGTEELQAILARAEHRMQARVMMALNSRAKSRFPPAMFYAPSDLGGLGMLSVGHSLIPAKDTIYSKTTSTGVQFFYAGLTNEEGIPIPNVLQYYTPWETEIKESEKAWLEFRTREREAKARGGRVSLDDIEDIIDKGIPRIRVRFSRHAPLFHFDTGFRARMEFQRYLAGKYLKNWWFHMEHDGNICGGVMERYRADMNNALGGVEAILEHSLFKGTGFPSWEGIEFDRSGGFENSKKDTKLAKQQRAGLSKVPNQRFALWWSPTINRSDIQAGFESKVETTGVFMCGKLETIKKSLIKIFSGSLWEKSHGAVVNDVASKLKDSLMDLGAASITLQQQHPQKSYTFTSSAADVILVSAARWSVSSKPTSLADEVGDVYQHSTTSKFWIDVQLRWGNYDSHNIAEYARKKFYEYSTARMYPFPAGIVVAIDLAYNCHSAFGYWIPGMKPFMSKLMPVIMKNNITLNTLRDRMKRDLGLFSSAPTEASLSDTNIAELFSSGMRTWIVDDSATYVTSEQPTPDGGKKFKSENGAVLVFEPVSGSLKMSVVHRSVFSGQKRRSKLAREKAAEEIASWLRSCPPSERPSKIIVTRSRFRQTLHNMLVLDYPNIIIGQSDLNLPLPMVLRHSRLVELRIAAMESKGWEFCIYDDWQQSLQFQPITCFNLLNLVLRGYHINLQRTRQILVPDLHVEVSASHFWPTYATRQEWEQVSIRLQEMIIADAARRMNVSPKDFTEKEKEGILLGKRMANVEIQQEEMKEIEAMRRTKLAETQTVNVVTNSGDVVKRKVKAAFDFGATALNNNWRPRSLADAAFLDENTPITFDAAGATGASDQLIFSEDAIQKLLACCDVKVQCCAYMFGHALPDSPNIKEVLCVMIPPQFGTAVEARTPPRIPFDAAALQEANLSFLGLMRIGESEAQLTSHDLALQARALIANEGMVPQGFVTAVLEMSEEGVMARCYSTTADGIAWAQSEHERLLKRTPEATDPSFSSPCRGTLSSEARSFFLVPADRVWNYFFKGALWRENTEYDVVVDVPLPFFHALHRPDHFLNFTRIGDGAEVVDETDPNDVFGNEAGLAGD

Target ORF information:

RefSeq Version	XM_001469165.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 U5 snRNA-associated splicing factor partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001469165.1

ORF Insert Sequence:

ATGAGCGTGT CGGTCCCTCC TGGGTTTGAG GACGATGACG ACCTGGCCAC TGTCGACCCT 
GCGCTACTTG CGGAGATGGA GGAGGAGGAG CAGAAGGAGT CGAGGAAGCA GCAGATCCTT 
ACATGGCAGC GCATGAACAC GGAGCGATAC GGCTTCCGCG CGGCGTACCG GGAGGCAGTG 
GGGGAGAAAG ACCTGATGCC GCCAGAGTTC ATCCGCAAGG TGGTGAAGGA CAATGGTGAC 
CTCGGCGGAA AGCGCTTCAA ATCGGAGCGC AAGCTGTGCG TGGGTATGCT GCCCTACATT 
CCACTGGCTC TGCTCAAGCT CCTAGAAAAC ATGCCAATGC CCTGGGAGGA GGCGCGTTAC 
GTGACCGTAG TGTACCACGT CGGAGGCGTG CTCACCATCG TGGACGACAC CCCCACAGTG 
GCGGAGCCGC TGTACGTGTC ACAGTGGGGT TCGATGTGGA CGAAGATGCG CGCCAACAAG 
GTAGAGCTGC TTCAAGAAGG TGGCGGCTTC AGGCGCGCGG TGCACAAGGG AGACGAAAAC 
GAGCCGCCGC TGGACTTTGC CGACTACCTC ATGGACCGCG TGCCGCCTCC ACCGGTGCTC 
GACGATCTCG ATGAGGAGGA CAACGCCAGC ATCGTGGGCT GGTTCTATGA CCCTTTTCCG 
CGCCTGGTGG CTCCCAACCA GATTCGCGGC CCTCGGCGGC CCAATGGGTA CTACTTCAAC 
CTTGATGTGA TGGAGGCGCT ATACCGCAAC GCGTCACCCA TTCTGCCGAC GCTTGACGAC 
CGCAACTACT TTTACCTGTG GGACCTCAAG TCCTTCTACG CTGCCAAAGC GATGCACCTG 
TCCATTCCAC GCGGCCCGAA GTTCGAGGCC CCACCTGCCA TTCGCGTGAA GCAGGACGAG 
GACGACGACT GGACGGAGTT CAACGACCTG CGTCGCATCA TCCACCGCGA CAATCCGCGC 
AAGCCGCGCT TCACGATGCT GACGGAGCGA CAGATTGCCT TCCCTTTCCT GTATGGCTCA 
GTGGTGGACG GGGTCGAACT GGCCCCGTAT CACTACCCAG CCACCGTGCG CGTCGAGAAC 
GACGACCCCG AGCTACCGTG CTTTACTTTC CACCCATCGC TGAACGCAAT CAAAGCAGTG 
GAGAAGAATT ACACAGACGT GCCGCGCGCG CAGGCCGCGT TGTGCTCCTC TACCTGGCAT 
CGCGCGGGCG GCCAGCATGT TGAGACCGCC GCGGACGTGC CGTTAGAGCT GCCGGACGAC 
TTTGAGCCGT TTCTGTCATC GCTCCCGCTG GAGCAGCCCA ACACGAAGAC GGGCCTGTCG 
CTGCTTTTTG CTCCGGCGCC CTACAACTCT TTTGAGGGAG GCATGAAGCG GCGCATCGAT 
GTGCCGCTGT GCGACAAGTG GTACCAGGAC CCACCGGACC TGCTCACGAC GGACGACCGA 
GACAAGATTC TGCGCAGCTA CACCCAGCTA CTCAAGCACC ACGTGAAGCG CTCCCTGCAG 
CACACCGTGC GCCAACGGAG CAGTTCCGCG ACCGCCGCCA CAGCAGCTGA AGGGGCGGGG 
ACGATGGACA TGAGCGCGAT GCAGGAGAAG CGCCGGCTGG AGAAAATGGC AGGGATGCGG 
TACTTTTCCA AGACGCGCAT GGAGTGGCTA GAAGCGGGGC TTCAGGTGAT GCGGCAGGGC 
CACAACATGC TTGTTCAGCT CATTAACATG AAGTGCCTGC CGTATGTGCA TATCGACTAC 
AACTTCGAGG CGAAGCCGAC CCGCACGCTG ACGACGAAGG AGATCAAGAA GAGCCGGCTC 
GGCCCCGCGT TTCACATGAT CCGTGAGCTG CTAGGCTTCA TGAAGCGGCT CATCGACATG 
CATGTGATGT ATCGGTTAGG GTCGACTGAC GCGCTGCAGT TGGCGGATGC TACCCAGTAC 
CTCTTCTCTC ACGTCGGCGT CCTGACGGGG GTGTACCGCT ACAAGTTGCG GGCTATGCGG 
CAGATCAAGA GGAGTCGTGA CCTTAAGCAC ATGCTGTACA GCAAGTTCAA CGTTGGCGGC 
GTGCCAAGTG GGCCTGGAAA CGGTTTTTGG GGCCCGGCGT GGCGTGTGTG GGTATTCTTT 
ATGCGCGGCA TGACACCGCT TCTTCAGCGC TATCTGAGCA ATCTGACCGA CCGCGTGCTG 
CACGGTCGTT TGCAGAAAGG CAAGATGTCG GGCAAGAAAA TCACGCGTCA GCGGGTCGAG 
CCGGACAAGG ACGTGAACAT CAAGGAGGCG TTCCGCCGTG AGCTGCGAGA GATGCTGCCG 
GACAACGTGA AGGCTTCGGT CATTGTGACG ATGGACCAGC ACATGAACGA GGCGTTCCGG 
CACTGGCGCG CCGGCCTGCC GTGGAGCGTG CCTGGGCTTG CGAAGCCGCT GACAGACCTC 
GTCGGTAAGT ACGTGAAGCT TCGCTCTGAG GAGTACATCC GCACGACGCA GCTGCAGCGG 
CGCCGCATTG CGGAGGGGGA TACTGTGGAC AAGCAGGCCT TCATGAAAAA CCTAGGGCGC 
TTGACGCGAC TGAAGCTCAT GGATGAGCAG CGCCGCCAGC GTCGATACAT GACAGGGGAG 
GACGCCGCCG CGATCATGGC GCCCGCTGAG GCGACGGAGA TTTATCGCAT GATGGCGAAC 
TGGCTCAACG ACCGCGGATT CAAGAAGATA TCCTTCCCAG ACCCGTCCAA GACGGCAGAG 
CTGTCGCTGC TGCAGCTTTC CCTCAATCGA CTGCGTGACC AGCACAACAT CGCGAACCGC 
CTCACGGCGA ACCAGCGTGA GGAGCAGGCC CGCATCGAGG AGTCCTTTAA CGCGCCTCAC 
GAGGCCCTGT CCCGCATCGT AGACGCCCTT GCACACCAGC GCCGCTTCAA AAACGTCGAG 
GTGGAGTACA TGGACAACTT TTCACACCTG TACCCGCTCT ACACTGTCGT GCCGCAGGAG 
AAGATGATTG ACTCCTTCCT CGATCAGTAC CTCTGGTACA AGGCGATGAA TGTGCAACGT 
CTCTTCCCCA ATTGGGTGAA ACCGTCCGAT GTGGAGCCGT TGCCACAGCT GGTGTACAAG 
TGGTGTGAGG GCATCAACAA CATCCCCGAT ATATGGGACG TATCGCACGA CGAGTCTGTG 
GTGCTGTTGC ACAGCAACCT GGAGGATGCA TTCTACGACA ACGTGGACTG GAACTTCTTC 
CGTCCGATGC TGGAGCTGAT CATGGATAAG TCGCTCGTCG ACTACATTGT GAGCCGGCAC 
GATGTCGTGG TGGAGTTCAA GGACATGAGC TACCATCACT ACAAGGGGCT TATCCGCGGC 
TTCATGTTTT CGTCATTCCT GGCGCAGTAC TGGGGGCTCG TGATGGATGT GCTGCTGCTC 
GGCACTCAGC GAAGCAAGGA GATTGCCGGT ACGGCGTTAA AGCCGAACCC ATTCATGTCT 
TTCTTCCGAG ACCCATACCT CGCCGCGTCG CATCCGATTC GCGCGTACTG CAGGTACAAG 
AACGAGGTCT ACATCGTGGT GCGCTACACC AAGACGGAGG CGGATGAGGT GCGGCGCCGC 
TACCTGGAGG AGACCAAGTC GGATCCGGAG ATGCGTGCGA TTAACGCATC CGTCTATGGA 
TTCAAGAACG CGAAGGAGTG GCCACGCGAC GCACGGATGC GCCTTTTCCT GTCCGACGTA 
AACTTGGCGC GCGCGGTGCT ATGGGAGTTC CGCAGCCGCC TGCCGCCGTC GATGGCGACC 
ATCAACGACG CAAACAGCTT CGTCAGCGTT TACTCCAAGG ACAACCCCAA CCTGCTCTTC 
GAAATGGCAG GCTTCTCCGT GCGGCTGCTG CCGGTGGTGC GGACCGAGGC GGACATTCTC 
GAGAGCGAGT CTATGTGGAG TCTGCGCAAT CACACAACCA AGGACATCTC CTGCCGAGCC 
TTCCTGCAAG TGACGAAGGA GCATATCAAC ATGATCCGCA ACAAGGCGCG CCGCACTGTA 
ATGATGGTCG GCAGCTCCAC CTTTCACAGC ATCGCGGCCA AGTGGAACGC GCTGATTACG 
GAGATTGTGC CATACTATCG CGAGGCGATT CTCGGGACGG AGGAGCTGCA GGCGATCCTG 
GCGCGCGCGG AGCACCGCAT GCAGGCGCGT GTGATGATGG CGCTGAACTC GCGCGCCAAG 
TCGCGCTTCC CACCGGCGAT GTTCTACGCC CCGTCCGATC TTGGCGGCCT CGGCATGCTC 
TCGGTCGGAC ACTCGCTGAT CCCTGCGAAG GACACCATCT ACTCGAAGAC GACCAGCACG 
GGCGTCCAGT TCTTCTACGC CGGCCTCACT AACGAGGAGG GCATCCCGAT CCCCAACGTG 
CTGCAGTATT ACACGCCGTG GGAAACGGAG ATCAAGGAGA GCGAGAAGGC ATGGCTGGAG 
TTCCGCACCC GCGAGCGTGA GGCCAAGGCG CGTGGGGGCC GCGTGTCGCT GGACGACATC 
GAGGACATCA TCGACAAGGG CATCCCGCGC ATCCGCGTGC GCTTCTCGCG ACACGCGCCG 
CTCTTCCACT TCGACACCGG CTTCCGGGCA CGCATGGAGT TCCAGCGATA CCTGGCCGGC 
AAGTACCTGA AGAACTGGTG GTTTCACATG GAGCACGACG GCAACATCTG CGGCGGCGTG 
ATGGAGCGCT ACCGCGCTGA TATGAACAAC GCCCTCGGTG GTGTGGAGGC GATTTTGGAG 
CACAGCCTCT TCAAGGGGAC GGGCTTTCCG TCGTGGGAAG GCATCGAGTT CGACCGCTCT 
GGCGGCTTTG AAAACTCGAA GAAGGATACA AAACTGGCGA AGCAGCAGCG TGCCGGTCTG 
AGCAAGGTGC CGAACCAGCG GTTCGCGCTT TGGTGGAGCC CTACGATCAA CCGCTCCGAC 
ATCCAGGCCG GCTTCGAGTC GAAGGTGGAG ACGACGGGTG TCTTCATGTG CGGTAAGCTG 
GAGACCATCA AGAAGTCGCT CATCAAGATC TTTAGCGGCT CTCTCTGGGA GAAGTCACAC 
GGCGCCGTCG TGAACGACGT GGCGAGCAAG CTGAAGGACA GCCTCATGGA CCTTGGCGCA 
GCCTCCATAA CGCTGCAACA GCAGCACCCA CAGAAGTCCT ACACCTTCAC GAGCTCGGCT 
GCCGATGTCA TATTGGTGAG CGCGGCGCGC TGGTCGGTAT CGTCGAAGCC GACCAGTCTC 
GCCGACGAGG TCGGCGACGT GTACCAACAC AGCACCACCT CGAAGTTCTG GATCGATGTG 
CAGCTGCGGT GGGGAAACTA CGACAGCCAC AACATTGCCG AGTACGCGAG GAAGAAGTTC 
TACGAGTACT CGACGGCGCG CATGTATCCG TTTCCTGCCG GCATTGTCGT TGCGATTGAC 
CTGGCCTACA ACTGCCACAG CGCGTTTGGC TACTGGATAC CTGGCATGAA GCCGTTCATG 
AGTAAGCTGA TGCCCGTCAT TATGAAGAAC AACATCACCC TCAACACGCT GCGCGACCGC 
ATGAAGCGCG ACCTGGGACT CTTCAGCTCT GCCCCGACGG AGGCGAGCCT GTCCGACACA 
AACATCGCTG AGCTGTTCTC CAGCGGGATG CGCACGTGGA TTGTAGACGA CAGCGCCACG 
TATGTGACGA GCGAGCAGCC GACCCCAGAC GGCGGCAAGA AGTTCAAGTC CGAGAATGGC 
GCGGTGCTCG TCTTTGAGCC CGTCTCCGGC TCTTTGAAGA TGAGCGTGGT GCACCGGAGC 
GTCTTTTCCG GGCAGAAGCG CCGCTCGAAG CTGGCGCGCG AGAAGGCAGC GGAGGAGATT 
GCGTCGTGGC TACGTAGCTG CCCGCCTAGC GAGCGTCCGA GCAAGATCAT CGTCACCCGC 
TCCCGCTTCC GCCAGACGCT GCATAACATG CTGGTGCTAG ACTACCCGAA CATCATCATT 
GGCCAAAGCG ACTTGAACCT GCCGCTGCCG ATGGTGTTGC GTCACAGCCG CCTCGTCGAG 
CTGCGCATCG CGGCGATGGA AAGCAAAGGG TGGGAGTTCT GCATCTACGA CGACTGGCAG 
CAGAGTCTGC AGTTCCAACC GATCACGTGC TTCAACCTAC TGAACCTGGT TCTGCGTGGC 
TACCACATAA ACCTGCAGCG CACACGGCAG ATTCTCGTGC CTGACCTGCA CGTCGAGGTA 
AGCGCGTCTC ACTTCTGGCC CACGTACGCG ACCCGACAGG AATGGGAGCA GGTGTCCATC 
CGTCTGCAGG AGATGATCAT CGCCGACGCC GCGCGCCGCA TGAACGTGAG CCCGAAGGAC 
TTCACGGAAA AGGAGAAGGA GGGCATTCTT CTGGGCAAGA GAATGGCCAA TGTGGAGATT 
CAGCAGGAGG AGATGAAGGA GATCGAGGCC ATGCGGCGCA CAAAACTGGC AGAGACGCAG 
ACGGTCAACG TTGTCACAAA CAGCGGCGAC GTCGTAAAGC GCAAGGTGAA GGCCGCCTTC 
GACTTTGGAG CGACGGCGTT GAACAACAAC TGGCGACCCC GCTCCTTGGC CGATGCCGCC 
TTTCTTGACG AGAACACTCC CATCACCTTC GACGCCGCCG GCGCCACCGG GGCAAGCGAC 
CAGCTCATCT TCTCTGAGGA CGCGATTCAG AAGCTGCTGG CGTGCTGCGA CGTTAAGGTT 
CAGTGCTGCG CGTACATGTT TGGCCATGCG CTGCCCGACT CGCCAAACAT CAAGGAGGTG 
CTGTGTGTCA TGATTCCGCC GCAGTTCGGG ACGGCTGTCG AGGCCCGCAC GCCACCACGC 
ATTCCGTTTG ACGCGGCAGC ACTGCAGGAG GCGAACCTGT CCTTCTTGGG ACTCATGAGG 
ATTGGCGAAA GCGAGGCGCA GCTGACCTCG CACGACCTCG CCCTCCAGGC GCGTGCGCTG 
ATCGCGAACG AGGGGATGGT GCCGCAAGGG TTTGTGACTG CCGTCCTGGA GATGTCGGAA 
GAGGGAGTGA TGGCGCGGTG CTACAGCACT ACCGCGGACG GCATTGCATG GGCGCAGAGT 
GAGCACGAGC GGCTGCTGAA GAGGACTCCG GAGGCGACGG ACCCATCCTT CTCCTCCCCG 
TGCCGCGGCA CCCTTTCCTC GGAGGCTCGT AGCTTCTTCC TGGTACCGGC GGATCGGGTC 
TGGAACTACT TCTTCAAGGG GGCACTGTGG CGCGAGAACA CCGAGTACGA CGTCGTCGTC 
GACGTGCCGT TGCCCTTCTT CCATGCGCTG CACCGCCCAG ACCACTTCCT TAACTTCACT 
CGCATCGGTG ACGGCGCCGA GGTGGTGGAT GAGACGGACC CAAACGACGT GTTCGGCAAC 
GAGGCCGGGC TGGCAGGTGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_35_4000 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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