LINJ_34_0380 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_34_0380
Entrez Gene ID	5072501
Full Name	putative DNA-directed RNA polymerase III largest subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001468388.1	XP_001468425.1	putative DNA-directed RNA polymerase III largest subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_34_0380 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_34_0380 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc05785	XM_001468388.1 Latest version!	Leishmania infantum JPCM5 putative DNA-directed RNA polymerase III largest subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_34_0380 ( XM_001468388.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001468425.1 Leishmania infantum JPCM5 putative DNA-directed RNA polymerase III largest subunit partial mRNA.

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CloneID	OLc05785
Clone ID Related Accession (Same CDS sequence)	XM_001468388.1
Accession Version	XM_001468388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative DNA-directed RNA polymerase III largest subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009418). COMPLETENESS: incomplete on both ends.

ATGTCTCAGA CTGCCACCAC GGCTGCGCAG TTTGTGCAGC CGCTGCTTGA TAAGCCGGTG 
GAGGTGACCT CGATCAAGTA CAGTCTCCTC TCTGAAGACA CGATCCACCG CCTCTCGGTC 
CTGCCCTGCC ACCGCGTGAT TGGGACCGAA AAGAACTTCG GCGTCAACGA TGGCCGCCTT 
GGCCCGAGTG ACCGCTTATC GGTGTGCAAC ACCTGCGGTC AGCGCAGCAT CGAGTGCACT 
GGCCACGCCG GCCACATCGA CCTCGAGGTG CCCGTCTTCC ACCTCGGCTA CTTCTCTGCA 
GTCATTCGAG TGTGCCGCAC AATCTGCAAG CGCTGCTCCA ACGTGCTCCT CACGCGAGAG 
GAAATGGAGT ACTACCTGCA CCGGCTGAGG TCCACGACAC ACGAGCCGCA GCAGCGCGCC 
GCCATCATCA AGGGCATCCA GGAGGACGCC TACAAGACGC GCGTGTGCCT CATGTGCGGT 
GGGCTGAACG GCACGGTGCG GCGGGTGCGG CCAATGCGCA TCGTCCATGA AAAGTACCTC 
GTCGATTTGC GCCGCGGCGA GCAGGCGAAT GAGGACCCGA CGGCGTTTTT CCAGGAGGAG 
CTGCGGTCTG CTGCGATGCA CAACTCGGAG GTGGGCGCGC ACAAGGAGTT CGTACACGAG 
TTCCTGCACC CGCAGCGTGT GAAGGAGCTT TTTGAAGCGA TTCCGCGGGA GCAGGTGCCG 
TTGCTAGGCC TGCAACCTGG CACCAGTCCA TGCAGCCTTC TCATCAGCAC CTTGCTCGTC 
CCACCCGTGT GCGTGCGGCC GCGCGGCATG TCGACGACGA ACCACATCCG CGAGGATGAC 
TTGACGACGC AGTACAACGA GATTCTAATC TGCTCTGACA TGATGAGCGA CGGCACAAAC 
GACGCCGCGC GCAGCGTAGA GACGTGGGAG CTGCTGCAGA CGCGTGTGGC GCGGCTGCTG 
GATGCGGCGC TGCCGGGGTT TCCTTCGCAC CTGCGCACCG TCGAGTGCAA GTCCTACGCG 
CAGCGCATGA AAGGCAAGCA GGGCCGTTTC CGGGGCAACC TTAGCGGCAA ACGCGTCGAC 
TTCTCTGGAC GGTCTGTCAT TTCACCCGAC CCAAACCTTC GCATCGACGA GCTTGCCGTG 
CCGCTGCGCG TCGCGCGTGT GCTGACGTAC CCGCAGCGCG TCTTTGCTGG CAACATCCAG 
CTCATGCGCC GGCTCGTGCG CAACGGCCCG CACGTGCACC CGGGCGCCCT CACAGTTTAC 
CTCTCGAAGG AGTGCTCGCG CAAGTCGCTG CGCAACGCCC GCGACCGTGA GGCCATCGCC 
GCGCGCCTCA GCATAGGCGA CGTCGTGGAG CGGCACGTGA TGAACGGCGA CTACATCCTC 
TTCAACCGCC AGCCGTCGCT CCACCGCATC TCGCTCATGG CGCACCGCGC GCGCGTGCTG 
CCCTTCCGCA CATTCCGGTT CAATGAGTGT TGCTGCGCGC CGTACAACGC CGACTTCGAC 
GGAGACGAGA TGAACATTCA CGTTGTGCAG ACGGAGGAGG CCCGCGCAGA GGCGAAGGAG 
CTGATGCTGA CGTCGCACAA CATCATCACT GCCAAGAACG GTGAGCCCAT CATCGCGTGC 
ACGCAGGACT TTCTGACCGC CGCCTACCTG GTGACCGCCC GTGACGCTCT TTTCGACCGT 
GCTTCCTTCA CGCAGATGGT GTCCCACTGG CTTGGCAACG AGACCCAGTA CACGCTGCCG 
ATTCCAGCCA TCCTGAAGCC GGCGGAGCTG TGGACCGGCA AGCAGCTCTT CGAGCTCATC 
CTTCGGCCAA CGCCAGAGAC GCAGCTGCTG CTGAACTTGG AAGCGAAGGC TCGCTTTTAC 
TCTGGTGCTG GCCGGCACGA CGATCCGGCG GAGGGGTATG TGGCGTTCCT CGACTCCGTC 
TTTCTCTCCG GCCGACTCGA CAAGAAGCTG CTGGGTGGCG GCGCCAAGGA CGGGCTCTTT 
GCCCGCCTCT ACGCTCTGGG CGGAGGGGAG CAGACGGCGA CGTGCATGTC GCGCATCGCG 
CAGTTCACCG CCCGCTACCT TCAGAACTAC GGCTTCTCCC TTGGTCTCGG TGATGTGTCG 
CCAACGCCGT CGCTCAACGA GAAGAAGGCC GCCGTGCTGG CTGTGTCGTT CGACAAGTGC 
GATCGTCTCA TCTCTCTCGC CAAGACGGGC CGGCTCATCC CCAAGCCTGG GATGAGCGTG 
AAGCAGAGCC TGGAGGCGAT GCTGAACGCG GAGCTGTCGC AGGTGCGTGA CGCGTGTGGC 
TCGGCCGCCG TGCAAGCGCT GGATGCCAAG TCGAACGCAC CTCTTATCAT GGTGAATTCT 
GGGAGCAAAG GCAGCGCCCT CAACACTGCA CAGATGATGG CGTGCGTGGG GCAGCAGACG 
GTCAGCGGCA AACGCATCAT GAACGCCTTC CAGGACCGCG CCCTGCCGCA CTTCCCGCGT 
TTCGCCGAAG ACCCCGCGTC GCGCGGCTTC GTGGCCAGCT CCTTCTACTC TGGCCTGTCG 
CCGACGGAGT TCTTCTTCCA TGGCATGGCT GGTCGCGAGG GTATTGTCGA TACAGCCGTC 
AAGACCGCAG AGACTGGCTA CATCTACCGC CGCCTCAGCA AGGCCATGGA GAACCTGAGC 
GTTGGCTACG ACGCCTCTGT GCGTAGCGTG CAGGGAAGCA TTGTTCAGCT GCGGTACGGC 
GAGGATGGCA TGGACCCGTG GCTGATGGAG GGGGCGCACG GCACGCCACT TAACCTCGAT 
CAGGAGTGGC TCAGCAACCG TGCCGCCTAC GCCCGCTTTC GAAAGCACAT CGACAATGTC 
TTGAGCGTGG GAGGGGGCAG TGGTGGGCTG GCCGCATCAG CGGATCCGCT CCTCCAAACG 
TACCGCAGGC TCTACGATGA ATGGCACGCC GTGTCCATGC TCCCTGGCGA GCTGGATACC 
TTCGTGCATC ACCTGCTGCA CGCGGATGAG GCGACGCTCG CCTCCTGCGC GCGCCTGATG 
AAGGCGGAGG CGGCGATACG CGGTGCGGCG CCGTCGTCTG CGGCCGCCGG TCTGCAGTTG 
TTACAGCAGC TCGCGCAGCA GCACGGCAGC GACCGCCTGC GCAGCGACAT TCAGGCGTTC 
TTCCGCAAGA AGCGCGACGA GCTGGTGCAG CTGCGGCAGA AACTGAAGCT ACCCCTGGAC 
GCCCATGCCC CTGCGGCCGC TGAGCTTGCA GCGGCGAGCG CTAAGGCAGT TCGTGGCGGG 
GAAAAGCGCA AGCCAAGTCC ACACAACGGC GTGCAGCCAT CCGTCAAAGC CGATGCGTCG 
GCCAGCGCAG CTGCCGTGCA GGCGTTTGCA GAGTCGCTTC AGGCCGAGCT GCTGCCACTC 
ACCAAAGGCA TGCTGCTTCA CTTCCTCGAG GCTTCAGCGC GCAAGGTGCA CCGCAAGCTG 
TGCGAGCCCG GCACGCCGTG CGGCGCCATC GCCGCTCAGT CTGTGGGTGA GCCGAGCACA 
CAGATGACGC TCCGCACGTT CCACTTTGCA GGTGTGGCCA GCATGAGTAT CACGCAGGGT 
GTGCCGCGCC TCGTGGAAAT CATCAATGCC AACAAGAGCA TCGCCACTCC GGTCATCACG 
GCTCCAATTG TGCTAGAGGA CGAATTCACG TCGATAGACC TCGCGCCGGC GAGTCGTACG 
CAGTACCAAG CCGCGCGCGC GGTGAAGGGT CTGATGGAAC GGGTGCTGCT GAAGGAGATC 
GCACGCGAAA TGGTTGAGGT TGTGACGCCG CGGGCGTATT ACATTCACAT CTTCCTCAAC 
ATGGAGCTCA TCGAGCGCCT CCTGCTGCCA ATCGATGCGG CGGTGGTGTG CCAGCGGCTC 
TACGCGGCGG CCGCGCGGCC AATGTCGCCG CTGCGCCATC TCTCCGAGGC GTGCGTCGAC 
GTGGTGAGCC GCGATTCCCT CGTCGTCAAG TCCTATGAGA AGGACCCGGC GCGGGTGCAT 
TTTAACGTTC AGCACCTGCT CACCCTCCTG CCCGATCTCG TGGTCGGTGG CGTCCCAGGC 
GTCAACCGCG TCATGATTGC CGACCGCTCG GAGAAGCTGC TGGCGGAGGG AGCGGAGCTC 
TTGTCGGTCA TGTCGCTGCC GTACGTGGAT GGTGTGCGCA CCACGTGCAA CCACGTCGCC 
GTCATAGAGC GCGCGCTCGG CATCGAGGCC GCCCGCGAGA CCATCGTGAA GGAAATTACG 
AGCATCTTGC AAGCCTATTC GCTCAACATC GACATTCGGC ACATCTACCT CCTGGCCGAC 
GTGATGACGA GTCGCGGCGT TGTTCTCGGC ATCACTCGAT ACGGCATCCA AAAGATGAAC 
AACAACGTCC TCACCATGGC GTCCTTCGAG CGCACCACGG AGCACTTGTA CAATGCGGCG 
ATGACGGAGA GGGAGGACGT CAACCTCAGC GTCTCCGAAA GCATCATCAT TGGCAAGCCG 
ATCCCCCTCG GCACCAGCAG CTTCAACATC CTGCTCGACA AGGGCAGCAT TGCGCCACCC 
GGCTCCAACA CGGGCCGAAT GCCGTCGCCC CGGCGCAGCG CCCACGTAGC AGCCACAGCG 
GCGGCGGCCT TGAAGTCGTC CGGCAAGGCT GCGGTGGCCG GCAAGCACAG CAAGAAGGTG 
GAGCCGACGC CGAAGGAGGT GTTCCTGCGC AGCTCTTTCA TGGCGTACGC CGCCGCGCAC 
GGGGCGGCGA CGCATCGAGG TCTGCGTGGC TGCTTTTCGA GTCGGTCCCT CTCCGCCGAG 
GGCGTCTTCC GGCTGGACCT CTTCGCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001468425.1
CDS	1..4773
Translation	MSQTATTAAQFVQPLLDKPVEVTSIKYSLLSEDTIHRLSVLPCHRVIGTEKNFGVNDGRLGPSDRLSVCNTCGQRSIECTGHAGHIDLEVPVFHLGYFSAVIRVCRTICKRCSNVLLTREEMEYYLHRLRSTTHEPQQRAAIIKGIQEDAYKTRVCLMCGGLNGTVRRVRPMRIVHEKYLVDLRRGEQANEDPTAFFQEELRSAAMHNSEVGAHKEFVHEFLHPQRVKELFEAIPREQVPLLGLQPGTSPCSLLISTLLVPPVCVRPRGMSTTNHIREDDLTTQYNEILICSDMMSDGTNDAARSVETWELLQTRVARLLDAALPGFPSHLRTVECKSYAQRMKGKQGRFRGNLSGKRVDFSGRSVISPDPNLRIDELAVPLRVARVLTYPQRVFAGNIQLMRRLVRNGPHVHPGALTVYLSKECSRKSLRNARDREAIAARLSIGDVVERHVMNGDYILFNRQPSLHRISLMAHRARVLPFRTFRFNECCCAPYNADFDGDEMNIHVVQTEEARAEAKELMLTSHNIITAKNGEPIIACTQDFLTAAYLVTARDALFDRASFTQMVSHWLGNETQYTLPIPAILKPAELWTGKQLFELILRPTPETQLLLNLEAKARFYSGAGRHDDPAEGYVAFLDSVFLSGRLDKKLLGGGAKDGLFARLYALGGGEQTATCMSRIAQFTARYLQNYGFSLGLGDVSPTPSLNEKKAAVLAVSFDKCDRLISLAKTGRLIPKPGMSVKQSLEAMLNAELSQVRDACGSAAVQALDAKSNAPLIMVNSGSKGSALNTAQMMACVGQQTVSGKRIMNAFQDRALPHFPRFAEDPASRGFVASSFYSGLSPTEFFFHGMAGREGIVDTAVKTAETGYIYRRLSKAMENLSVGYDASVRSVQGSIVQLRYGEDGMDPWLMEGAHGTPLNLDQEWLSNRAAYARFRKHIDNVLSVGGGSGGLAASADPLLQTYRRLYDEWHAVSMLPGELDTFVHHLLHADEATLASCARLMKAEAAIRGAAPSSAAAGLQLLQQLAQQHGSDRLRSDIQAFFRKKRDELVQLRQKLKLPLDAHAPAAAELAAASAKAVRGGEKRKPSPHNGVQPSVKADASASAAAVQAFAESLQAELLPLTKGMLLHFLEASARKVHRKLCEPGTPCGAIAAQSVGEPSTQMTLRTFHFAGVASMSITQGVPRLVEIINANKSIATPVITAPIVLEDEFTSIDLAPASRTQYQAARAVKGLMERVLLKEIAREMVEVVTPRAYYIHIFLNMELIERLLLPIDAAVVCQRLYAAAARPMSPLRHLSEACVDVVSRDSLVVKSYEKDPARVHFNVQHLLTLLPDLVVGGVPGVNRVMIADRSEKLLAEGAELLSVMSLPYVDGVRTTCNHVAVIERALGIEAARETIVKEITSILQAYSLNIDIRHIYLLADVMTSRGVVLGITRYGIQKMNNNVLTMASFERTTEHLYNAAMTEREDVNLSVSESIIIGKPIPLGTSSFNILLDKGSIAPPGSNTGRMPSPRRSAHVAATAAAALKSSGKAAVAGKHSKKVEPTPKEVFLRSSFMAYAAAHGAATHRGLRGCFSSRSLSAEGVFRLDLFAL

Target ORF information:

RefSeq Version	XM_001468388.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative DNA-directed RNA polymerase III largest subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001468388.1

ORF Insert Sequence:

ATGTCTCAGA CTGCCACCAC GGCTGCGCAG TTTGTGCAGC CGCTGCTTGA TAAGCCGGTG 
GAGGTGACCT CGATCAAGTA CAGTCTCCTC TCTGAAGACA CGATCCACCG CCTCTCGGTC 
CTGCCCTGCC ACCGCGTGAT TGGGACCGAA AAGAACTTCG GCGTCAACGA TGGCCGCCTT 
GGCCCGAGTG ACCGCTTATC GGTGTGCAAC ACCTGCGGTC AGCGCAGCAT CGAGTGCACT 
GGCCACGCCG GCCACATCGA CCTCGAGGTG CCCGTCTTCC ACCTCGGCTA CTTCTCTGCA 
GTCATTCGAG TGTGCCGCAC AATCTGCAAG CGCTGCTCCA ACGTGCTCCT CACGCGAGAG 
GAAATGGAGT ACTACCTGCA CCGGCTGAGG TCCACGACAC ACGAGCCGCA GCAGCGCGCC 
GCCATCATCA AGGGCATCCA GGAGGACGCC TACAAGACGC GCGTGTGCCT CATGTGCGGT 
GGGCTGAACG GCACGGTGCG GCGGGTGCGG CCAATGCGCA TCGTCCATGA AAAGTACCTC 
GTCGATTTGC GCCGCGGCGA GCAGGCGAAT GAGGACCCGA CGGCGTTTTT CCAGGAGGAG 
CTGCGGTCTG CTGCGATGCA CAACTCGGAG GTGGGCGCGC ACAAGGAGTT CGTACACGAG 
TTCCTGCACC CGCAGCGTGT GAAGGAGCTT TTTGAAGCGA TTCCGCGGGA GCAGGTGCCG 
TTGCTAGGCC TGCAACCTGG CACCAGTCCA TGCAGCCTTC TCATCAGCAC CTTGCTCGTC 
CCACCCGTGT GCGTGCGGCC GCGCGGCATG TCGACGACGA ACCACATCCG CGAGGATGAC 
TTGACGACGC AGTACAACGA GATTCTAATC TGCTCTGACA TGATGAGCGA CGGCACAAAC 
GACGCCGCGC GCAGCGTAGA GACGTGGGAG CTGCTGCAGA CGCGTGTGGC GCGGCTGCTG 
GATGCGGCGC TGCCGGGGTT TCCTTCGCAC CTGCGCACCG TCGAGTGCAA GTCCTACGCG 
CAGCGCATGA AAGGCAAGCA GGGCCGTTTC CGGGGCAACC TTAGCGGCAA ACGCGTCGAC 
TTCTCTGGAC GGTCTGTCAT TTCACCCGAC CCAAACCTTC GCATCGACGA GCTTGCCGTG 
CCGCTGCGCG TCGCGCGTGT GCTGACGTAC CCGCAGCGCG TCTTTGCTGG CAACATCCAG 
CTCATGCGCC GGCTCGTGCG CAACGGCCCG CACGTGCACC CGGGCGCCCT CACAGTTTAC 
CTCTCGAAGG AGTGCTCGCG CAAGTCGCTG CGCAACGCCC GCGACCGTGA GGCCATCGCC 
GCGCGCCTCA GCATAGGCGA CGTCGTGGAG CGGCACGTGA TGAACGGCGA CTACATCCTC 
TTCAACCGCC AGCCGTCGCT CCACCGCATC TCGCTCATGG CGCACCGCGC GCGCGTGCTG 
CCCTTCCGCA CATTCCGGTT CAATGAGTGT TGCTGCGCGC CGTACAACGC CGACTTCGAC 
GGAGACGAGA TGAACATTCA CGTTGTGCAG ACGGAGGAGG CCCGCGCAGA GGCGAAGGAG 
CTGATGCTGA CGTCGCACAA CATCATCACT GCCAAGAACG GTGAGCCCAT CATCGCGTGC 
ACGCAGGACT TTCTGACCGC CGCCTACCTG GTGACCGCCC GTGACGCTCT TTTCGACCGT 
GCTTCCTTCA CGCAGATGGT GTCCCACTGG CTTGGCAACG AGACCCAGTA CACGCTGCCG 
ATTCCAGCCA TCCTGAAGCC GGCGGAGCTG TGGACCGGCA AGCAGCTCTT CGAGCTCATC 
CTTCGGCCAA CGCCAGAGAC GCAGCTGCTG CTGAACTTGG AAGCGAAGGC TCGCTTTTAC 
TCTGGTGCTG GCCGGCACGA CGATCCGGCG GAGGGGTATG TGGCGTTCCT CGACTCCGTC 
TTTCTCTCCG GCCGACTCGA CAAGAAGCTG CTGGGTGGCG GCGCCAAGGA CGGGCTCTTT 
GCCCGCCTCT ACGCTCTGGG CGGAGGGGAG CAGACGGCGA CGTGCATGTC GCGCATCGCG 
CAGTTCACCG CCCGCTACCT TCAGAACTAC GGCTTCTCCC TTGGTCTCGG TGATGTGTCG 
CCAACGCCGT CGCTCAACGA GAAGAAGGCC GCCGTGCTGG CTGTGTCGTT CGACAAGTGC 
GATCGTCTCA TCTCTCTCGC CAAGACGGGC CGGCTCATCC CCAAGCCTGG GATGAGCGTG 
AAGCAGAGCC TGGAGGCGAT GCTGAACGCG GAGCTGTCGC AGGTGCGTGA CGCGTGTGGC 
TCGGCCGCCG TGCAAGCGCT GGATGCCAAG TCGAACGCAC CTCTTATCAT GGTGAATTCT 
GGGAGCAAAG GCAGCGCCCT CAACACTGCA CAGATGATGG CGTGCGTGGG GCAGCAGACG 
GTCAGCGGCA AACGCATCAT GAACGCCTTC CAGGACCGCG CCCTGCCGCA CTTCCCGCGT 
TTCGCCGAAG ACCCCGCGTC GCGCGGCTTC GTGGCCAGCT CCTTCTACTC TGGCCTGTCG 
CCGACGGAGT TCTTCTTCCA TGGCATGGCT GGTCGCGAGG GTATTGTCGA TACAGCCGTC 
AAGACCGCAG AGACTGGCTA CATCTACCGC CGCCTCAGCA AGGCCATGGA GAACCTGAGC 
GTTGGCTACG ACGCCTCTGT GCGTAGCGTG CAGGGAAGCA TTGTTCAGCT GCGGTACGGC 
GAGGATGGCA TGGACCCGTG GCTGATGGAG GGGGCGCACG GCACGCCACT TAACCTCGAT 
CAGGAGTGGC TCAGCAACCG TGCCGCCTAC GCCCGCTTTC GAAAGCACAT CGACAATGTC 
TTGAGCGTGG GAGGGGGCAG TGGTGGGCTG GCCGCATCAG CGGATCCGCT CCTCCAAACG 
TACCGCAGGC TCTACGATGA ATGGCACGCC GTGTCCATGC TCCCTGGCGA GCTGGATACC 
TTCGTGCATC ACCTGCTGCA CGCGGATGAG GCGACGCTCG CCTCCTGCGC GCGCCTGATG 
AAGGCGGAGG CGGCGATACG CGGTGCGGCG CCGTCGTCTG CGGCCGCCGG TCTGCAGTTG 
TTACAGCAGC TCGCGCAGCA GCACGGCAGC GACCGCCTGC GCAGCGACAT TCAGGCGTTC 
TTCCGCAAGA AGCGCGACGA GCTGGTGCAG CTGCGGCAGA AACTGAAGCT ACCCCTGGAC 
GCCCATGCCC CTGCGGCCGC TGAGCTTGCA GCGGCGAGCG CTAAGGCAGT TCGTGGCGGG 
GAAAAGCGCA AGCCAAGTCC ACACAACGGC GTGCAGCCAT CCGTCAAAGC CGATGCGTCG 
GCCAGCGCAG CTGCCGTGCA GGCGTTTGCA GAGTCGCTTC AGGCCGAGCT GCTGCCACTC 
ACCAAAGGCA TGCTGCTTCA CTTCCTCGAG GCTTCAGCGC GCAAGGTGCA CCGCAAGCTG 
TGCGAGCCCG GCACGCCGTG CGGCGCCATC GCCGCTCAGT CTGTGGGTGA GCCGAGCACA 
CAGATGACGC TCCGCACGTT CCACTTTGCA GGTGTGGCCA GCATGAGTAT CACGCAGGGT 
GTGCCGCGCC TCGTGGAAAT CATCAATGCC AACAAGAGCA TCGCCACTCC GGTCATCACG 
GCTCCAATTG TGCTAGAGGA CGAATTCACG TCGATAGACC TCGCGCCGGC GAGTCGTACG 
CAGTACCAAG CCGCGCGCGC GGTGAAGGGT CTGATGGAAC GGGTGCTGCT GAAGGAGATC 
GCACGCGAAA TGGTTGAGGT TGTGACGCCG CGGGCGTATT ACATTCACAT CTTCCTCAAC 
ATGGAGCTCA TCGAGCGCCT CCTGCTGCCA ATCGATGCGG CGGTGGTGTG CCAGCGGCTC 
TACGCGGCGG CCGCGCGGCC AATGTCGCCG CTGCGCCATC TCTCCGAGGC GTGCGTCGAC 
GTGGTGAGCC GCGATTCCCT CGTCGTCAAG TCCTATGAGA AGGACCCGGC GCGGGTGCAT 
TTTAACGTTC AGCACCTGCT CACCCTCCTG CCCGATCTCG TGGTCGGTGG CGTCCCAGGC 
GTCAACCGCG TCATGATTGC CGACCGCTCG GAGAAGCTGC TGGCGGAGGG AGCGGAGCTC 
TTGTCGGTCA TGTCGCTGCC GTACGTGGAT GGTGTGCGCA CCACGTGCAA CCACGTCGCC 
GTCATAGAGC GCGCGCTCGG CATCGAGGCC GCCCGCGAGA CCATCGTGAA GGAAATTACG 
AGCATCTTGC AAGCCTATTC GCTCAACATC GACATTCGGC ACATCTACCT CCTGGCCGAC 
GTGATGACGA GTCGCGGCGT TGTTCTCGGC ATCACTCGAT ACGGCATCCA AAAGATGAAC 
AACAACGTCC TCACCATGGC GTCCTTCGAG CGCACCACGG AGCACTTGTA CAATGCGGCG 
ATGACGGAGA GGGAGGACGT CAACCTCAGC GTCTCCGAAA GCATCATCAT TGGCAAGCCG 
ATCCCCCTCG GCACCAGCAG CTTCAACATC CTGCTCGACA AGGGCAGCAT TGCGCCACCC 
GGCTCCAACA CGGGCCGAAT GCCGTCGCCC CGGCGCAGCG CCCACGTAGC AGCCACAGCG 
GCGGCGGCCT TGAAGTCGTC CGGCAAGGCT GCGGTGGCCG GCAAGCACAG CAAGAAGGTG 
GAGCCGACGC CGAAGGAGGT GTTCCTGCGC AGCTCTTTCA TGGCGTACGC CGCCGCGCAC 
GGGGCGGCGA CGCATCGAGG TCTGCGTGGC TGCTTTTCGA GTCGGTCCCT CTCCGCCGAG 
GGCGTCTTCC GGCTGGACCT CTTCGCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_34_0380 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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