LINJ_33_2130 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_33_2130
Entrez Gene ID	5072357
Full Name	calpain protease-like protein
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001468245.2	XP_001468282.2	calpain protease-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_33_2130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_33_2130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc05645	XM_001468245.2 Latest version!	Leishmania infantum JPCM5 calpain protease-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_33_2130 ( XM_001468245.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001468282.2 Leishmania infantum JPCM5 calpain protease-like protein partial mRNA.

Price & Availability↑

CloneID	OLc05645
Clone ID Related Accession (Same CDS sequence)	XM_001468245.2
Accession Version	XM_001468245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	calpain protease-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009417). On Jul 14, 2011 this sequence version replaced XM_001468245.1. COMPLETENESS: incomplete on both ends.

ATGCCGTCCT CCTTCCCTGC TGAGGCGTCC ATGTGGATGC AGAACGCCGT TCACCTCGAT 
ACGGACCACA AATACGTGGA GGCGGAGGAG TGCTACACGA AGGCCATTTT TTTGTTTCGG 
CAGGCCCTCT CGGACCCGAA CATGGCGGAC ACCGAGCGAC AGAAGCTCTT GAGCTACATG 
AGTGATGTAG CCAAGCGCAT ACAGCGGCTG CGCGCGCTGA ACCGCACTTC CCAGAGTGCG 
AACTCGCCTG AGCCTAGCGT TTCTCCGTCC GGCCCGTTGG GACGCCGTGC CTCGGCTAGG 
CAGGCAAGCG AGCACACGCC TCCTCGTGGC TCCGACGCCG CCGCGACGGC CTTCGGCGCG 
CGGAACCCAC GCGGTAAGGA CGGCAAAAAG GCACCAGCAG GCGTCGAAAA CGACTTGAAA 
GTGCTGGACG CCATTCAAGT CAATCAAACC GATCCATTTC AGGCCAGCAA CTCCAGCCCC 
AGCCCCTCCG CCGCGTCGTC AATGTCGGGC AAGCGCGCGT TGCCGGTGGC TTCTCCGACC 
CCTTCACCCC CGTCAGCGCC GCCATGTAAG CCAACCTGCG GCAGCGACGG CGTGCCAGAT 
GGTGGCAAGC CAGGCGGCAG CTCCGCGGAA CCGCCCGCGT TGCCGACTCC GCCACCACCT 
GGCTCCTCGC AGCGGCTGTT CGGCGTCGCG TCTCACTTTG CCCCTCGTAC ATCCGAGATG 
GTTTACAAGG CGATTGGGAT CGCGATTCAG CTTAGCCAGC AAGGGGAGCT CAAGCGGGCG 
GTAGATGTGC TGCAGCACGC CTACTCGCGG GGCAGCCGTG AGCGCAATAA CCCTGGCAAT 
TTCAAGGAGA TTGGCGAGAC ACTGAAGCTA ATGCGCCAGA AGTACTACGC AAAACATCTG 
CCACGCTTCC TGCAGGACAA CCCGATCCTG CCGAGCGAGA TGGAGCTGCT GCGCAAGAGC 
GGCATCACAA GCACGATCCT GCTCCCGCTC TGGGACGATG TGCATGAGGG CTACGGAGCT 
GAGAACATCT TCATGCCCTG CGAGGGAGTT TGGGAGGACG CTTTCACACC AAAGCTGTGC 
CAGAGGCAAA TCAGCTCCGG CGCCGTCCTC ATGCACTTTT CCGGATACCG GCAGGCGGGG 
CTTGACTACA CCATTGTGCG CGAGGCGGAT CCACTGCACA TTCGGCAGAG CGTCGTCGGC 
GACTGCTCAC TCGTCTGCAG TCTCATGATC TGCGCCAGCT ATCAGAAGCG CTTCCCTAAC 
GCGAAGATTA TCAGCAACGT CATCTACCCT CAAGACCGCG ACGGCAACCC AATCTTGAAC 
CCGAAGGGCA AGTACTGTGT AAAGATGCTC ATCAACGGTA TTACTCGCAT GGTCACGGTG 
GACGATCGGC TGCCGGTGAA CCCTCAGTCG AGGCGCTTCC TCTGCACGAG CAGCACGGAT 
CCGAGCGAGC TGTGGGTATC CATTATGGAA AAGGCGTTCG TCAAGGTGTG CGGCGGCAGC 
TACAACTTCC CCGGCAGCGT CAGCTACGCC GACCTGTACA AGCTAAGCGG GTGGCTGCCA 
GACTCGACGG CGTTCGGCAA GCCCGAGTTC GACCGCGAGT TCCAGTGGCG GCGGCTTTAT 
CAAAACTTCA AGGCTGGTGC TCTGCTCTTC ACAGTCAGCA CCCCTGTGGA GATGCCAGCA 
GCGGACGAAG CGCTCGGGCT CGCCGCAGGG CACGCGTATG CGGTGCTGGA TATGCAGGAG 
GTGGGCAAGG AGCGCGTCAT GCTGCTGCGC AACCCGTGGG GGAAGCAGGA GTGGAACGGC 
AAGTACGGCC GCCGCGACAC CAGCGAGGCA AGCGTCGCCG TCCGCGCCGC CTTTGATCTG 
AAACGCGAGG AGGAGGACAT GGGTGTCTTC TGCATCGCCT ACGATGATGT GGTGCGCTGC 
TTCGACAACT GCAGTCTGAG CTGGAACCCA TACATGCTGT ACCGCACCCC GGAGGGTCGG 
CCGCGCCGAC CGGTGCGCAT CGCGTGCCAC GGCGCGTTCG ACTACACCCT CAGCACGGCG 
CTGTCGCCGC AGCTGCACAT TGGTGTCGTC GACGCGCCGA AGCGGACGCG CATGCACCTG 
ATCTTGTCTC GCCACATCAC CGACGTCTCG GAGTTTGGCC GGCAGTACGA GAAGGACGAC 
GACACGACAC CGTACCTGGC CTTGAAGGTG TACGACGTGA CCGACTACCC GTCTGTAGCG 
CAGCACCTTG GTGGCCATTG CTCGCTGGAG ATGTGCTACT GCCGCCGCCT CGCCTCGAAT 
TCGGACTTTA AGAATAGCAT TGAGCCCCTC AACGACGTCA TCTACCGCGC CTCGTCTGCG 
CGCACCTTCA GCTTCGACTG TCCTGCCGGC TCGAGCAATC TGGTGGTGGT CGTGTCGCGG 
ACTGCCTCAA AGGCGCGGCA GCCTTTCAAC TTTACGGTGA CCCTGCACAC GGAGCTGGAG 
CAGATGCGGC TGCCGCGGCT GCAGGGCAAC TACGGCAGCG GCGCCGAGGA CAAGAACTCC 
CTCCTGGCAA GCGCCAACGC CAACGCCGGG AACCCGTGCG CTGGAGTGTA CATGCACATG 
ATACCGACCT CCTCGCTCAA GTTCACGACG CGGCTGTCCG GCGCGTGGGT GAAGGGCAAG 
ACTTGCGGCG GCCGCACCGA CGCCGCCACG TATGTCTTCA ACCCGCAGTA CCGCCTGCAC 
CTGGACCAGC CCTCGCACGT GTCGCTGAGG CTGGCTGTGG CAGGATGCGA GGTGCCGTGC 
TCGATTCAGA TGATCAAGCC GATGTCGTCC AACAAATACG AGGAGAACGG CGGCAGCGGT 
GGAATCAAGC GCAAGATGAC TGACTTCGAC GGCCGTGTCG GTAACGGTGC CAAGGCGGGC 
GAGCTTGTTC TCGAGAGCGC CCGCTACGCC TTTGGTGGGG CCGTTCTAGA TAGCGCGCTG 
CCGTTCTGCG TCTGCTACGA CCGCTACCGC GCCCTCGGCA AGCTGCATTG GAGGGAAGCG 
AAGAAGGTGC TGGTGAACGT GACCGCCCCG AACCGGACGC CTGAGTCCTT CATCGTCTAC 
GACCTCGGGG ACACCGACAC CGTGGCGGTG CTTCTCCTGA ATGCTATCAA GAAGGGCTGC 
GCTGCCTCCC CACGTGACGC GCACTTTAGC GTGAACGGGA AGCCGATCAC GCTCGAGGCC 
ACCATCGCGT CGCTCTTCCA GCTCGCATCC TCCCTCGGCG GCGAGTCCCT CGCCTCTGGC 
GGGGATGGCG CGACAACCGC GAAGTCTGTC ACAGTAGCCC TCGCCCTGGA CGGCAAGGCG 
GCGTCGATCG AGTCTGCCTC GAGCCCACCT GTGAGGAAGC TGAAGGAAGT CATGAACGAC 
ATAGTGTCTG ATGTGCTCAA GTGCTCGTCG CAGTTCAGCA GCTACGTGCC ACGCTTGACG 
CATCTTGCCA GGCAGGCAAA CAGCGCGCTG ACCGGTGCCA GCGATGCGGA CGTGCGAGCG 
TACATTCTCG AGCTCGCCGC TTACCTTGCT GACTACGTGA AGCGGTGTGC CAGCGCGGTG 
CGGTCGGCGC AGACGCCGCA GGCGCTGCTT CCGCTGCTTC CTGCCGGCGA CTACACGGTC 
ATTCCATCTT TGTGGGAGAA GGGTAAGCCG GGCAGCTTTG AGCTGACGGT GGAGACGTCA 
CAGCCACATA AGACGTCGGC GATTCCGAAG GAGGGACACA ACATGCAAGA GACGCTTGTG 
CGCGGCGCAC TACGCCCCGT CGCGGTTTGC CTGGGCACGT CACTGATGAA GCCGAGAGCA 
GTGCTCAGCA TGAGGGATCC GTTCTTTGAG AACAGCAAGG TGCAGGTGCT GTGCCCCGCC 
CGCTCCGTCT TCATGAGTCG TCTCTTTGTC CTGGTTGACA TGGTGGATGC GGAGGCGAAG 
GGCCAGCCGG CCAAGGCCCC CGCCACGAAT CTGAGCCTCT TCCGTGTGCA CAACGCGTCA 
TCGATGGAGC TGGTGTGCGC TTCAGACGAG TACTCCCATG GCGGTGTGTC AACCCCACCT 
GTAGAGCTGG AGGCGAAGAC GCAGTATCTG CTCGTGGTGA GCGCAAAGGA GAGCGTGGCT 
GACAAGTTCC TCCTCCGCAT CTATACCAGC GGCAGCTGCA CCGCTCAGCT GGCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001468282.2
CDS	1..4140
Translation	MPSSFPAEASMWMQNAVHLDTDHKYVEAEECYTKAIFLFRQALSDPNMADTERQKLLSYMSDVAKRIQRLRALNRTSQSANSPEPSVSPSGPLGRRASARQASEHTPPRGSDAAATAFGARNPRGKDGKKAPAGVENDLKVLDAIQVNQTDPFQASNSSPSPSAASSMSGKRALPVASPTPSPPSAPPCKPTCGSDGVPDGGKPGGSSAEPPALPTPPPPGSSQRLFGVASHFAPRTSEMVYKAIGIAIQLSQQGELKRAVDVLQHAYSRGSRERNNPGNFKEIGETLKLMRQKYYAKHLPRFLQDNPILPSEMELLRKSGITSTILLPLWDDVHEGYGAENIFMPCEGVWEDAFTPKLCQRQISSGAVLMHFSGYRQAGLDYTIVREADPLHIRQSVVGDCSLVCSLMICASYQKRFPNAKIISNVIYPQDRDGNPILNPKGKYCVKMLINGITRMVTVDDRLPVNPQSRRFLCTSSTDPSELWVSIMEKAFVKVCGGSYNFPGSVSYADLYKLSGWLPDSTAFGKPEFDREFQWRRLYQNFKAGALLFTVSTPVEMPAADEALGLAAGHAYAVLDMQEVGKERVMLLRNPWGKQEWNGKYGRRDTSEASVAVRAAFDLKREEEDMGVFCIAYDDVVRCFDNCSLSWNPYMLYRTPEGRPRRPVRIACHGAFDYTLSTALSPQLHIGVVDAPKRTRMHLILSRHITDVSEFGRQYEKDDDTTPYLALKVYDVTDYPSVAQHLGGHCSLEMCYCRRLASNSDFKNSIEPLNDVIYRASSARTFSFDCPAGSSNLVVVVSRTASKARQPFNFTVTLHTELEQMRLPRLQGNYGSGAEDKNSLLASANANAGNPCAGVYMHMIPTSSLKFTTRLSGAWVKGKTCGGRTDAATYVFNPQYRLHLDQPSHVSLRLAVAGCEVPCSIQMIKPMSSNKYEENGGSGGIKRKMTDFDGRVGNGAKAGELVLESARYAFGGAVLDSALPFCVCYDRYRALGKLHWREAKKVLVNVTAPNRTPESFIVYDLGDTDTVAVLLLNAIKKGCAASPRDAHFSVNGKPITLEATIASLFQLASSLGGESLASGGDGATTAKSVTVALALDGKAASIESASSPPVRKLKEVMNDIVSDVLKCSSQFSSYVPRLTHLARQANSALTGASDADVRAYILELAAYLADYVKRCASAVRSAQTPQALLPLLPAGDYTVIPSLWEKGKPGSFELTVETSQPHKTSAIPKEGHNMQETLVRGALRPVAVCLGTSLMKPRAVLSMRDPFFENSKVQVLCPARSVFMSRLFVLVDMVDAEAKGQPAKAPATNLSLFRVHNASSMELVCASDEYSHGGVSTPPVELEAKTQYLLVVSAKESVADKFLLRIYTSGSCTAQLAS

Target ORF information:

RefSeq Version	XM_001468245.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 calpain protease-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001468245.2

ORF Insert Sequence:

ATGCCGTCCT CCTTCCCTGC TGAGGCGTCC ATGTGGATGC AGAACGCCGT TCACCTCGAT 
ACGGACCACA AATACGTGGA GGCGGAGGAG TGCTACACGA AGGCCATTTT TTTGTTTCGG 
CAGGCCCTCT CGGACCCGAA CATGGCGGAC ACCGAGCGAC AGAAGCTCTT GAGCTACATG 
AGTGATGTAG CCAAGCGCAT ACAGCGGCTG CGCGCGCTGA ACCGCACTTC CCAGAGTGCG 
AACTCGCCTG AGCCTAGCGT TTCTCCGTCC GGCCCGTTGG GACGCCGTGC CTCGGCTAGG 
CAGGCAAGCG AGCACACGCC TCCTCGTGGC TCCGACGCCG CCGCGACGGC CTTCGGCGCG 
CGGAACCCAC GCGGTAAGGA CGGCAAAAAG GCACCAGCAG GCGTCGAAAA CGACTTGAAA 
GTGCTGGACG CCATTCAAGT CAATCAAACC GATCCATTTC AGGCCAGCAA CTCCAGCCCC 
AGCCCCTCCG CCGCGTCGTC AATGTCGGGC AAGCGCGCGT TGCCGGTGGC TTCTCCGACC 
CCTTCACCCC CGTCAGCGCC GCCATGTAAG CCAACCTGCG GCAGCGACGG CGTGCCAGAT 
GGTGGCAAGC CAGGCGGCAG CTCCGCGGAA CCGCCCGCGT TGCCGACTCC GCCACCACCT 
GGCTCCTCGC AGCGGCTGTT CGGCGTCGCG TCTCACTTTG CCCCTCGTAC ATCCGAGATG 
GTTTACAAGG CGATTGGGAT CGCGATTCAG CTTAGCCAGC AAGGGGAGCT CAAGCGGGCG 
GTAGATGTGC TGCAGCACGC CTACTCGCGG GGCAGCCGTG AGCGCAATAA CCCTGGCAAT 
TTCAAGGAGA TTGGCGAGAC ACTGAAGCTA ATGCGCCAGA AGTACTACGC AAAACATCTG 
CCACGCTTCC TGCAGGACAA CCCGATCCTG CCGAGCGAGA TGGAGCTGCT GCGCAAGAGC 
GGCATCACAA GCACGATCCT GCTCCCGCTC TGGGACGATG TGCATGAGGG CTACGGAGCT 
GAGAACATCT TCATGCCCTG CGAGGGAGTT TGGGAGGACG CTTTCACACC AAAGCTGTGC 
CAGAGGCAAA TCAGCTCCGG CGCCGTCCTC ATGCACTTTT CCGGATACCG GCAGGCGGGG 
CTTGACTACA CCATTGTGCG CGAGGCGGAT CCACTGCACA TTCGGCAGAG CGTCGTCGGC 
GACTGCTCAC TCGTCTGCAG TCTCATGATC TGCGCCAGCT ATCAGAAGCG CTTCCCTAAC 
GCGAAGATTA TCAGCAACGT CATCTACCCT CAAGACCGCG ACGGCAACCC AATCTTGAAC 
CCGAAGGGCA AGTACTGTGT AAAGATGCTC ATCAACGGTA TTACTCGCAT GGTCACGGTG 
GACGATCGGC TGCCGGTGAA CCCTCAGTCG AGGCGCTTCC TCTGCACGAG CAGCACGGAT 
CCGAGCGAGC TGTGGGTATC CATTATGGAA AAGGCGTTCG TCAAGGTGTG CGGCGGCAGC 
TACAACTTCC CCGGCAGCGT CAGCTACGCC GACCTGTACA AGCTAAGCGG GTGGCTGCCA 
GACTCGACGG CGTTCGGCAA GCCCGAGTTC GACCGCGAGT TCCAGTGGCG GCGGCTTTAT 
CAAAACTTCA AGGCTGGTGC TCTGCTCTTC ACAGTCAGCA CCCCTGTGGA GATGCCAGCA 
GCGGACGAAG CGCTCGGGCT CGCCGCAGGG CACGCGTATG CGGTGCTGGA TATGCAGGAG 
GTGGGCAAGG AGCGCGTCAT GCTGCTGCGC AACCCGTGGG GGAAGCAGGA GTGGAACGGC 
AAGTACGGCC GCCGCGACAC CAGCGAGGCA AGCGTCGCCG TCCGCGCCGC CTTTGATCTG 
AAACGCGAGG AGGAGGACAT GGGTGTCTTC TGCATCGCCT ACGATGATGT GGTGCGCTGC 
TTCGACAACT GCAGTCTGAG CTGGAACCCA TACATGCTGT ACCGCACCCC GGAGGGTCGG 
CCGCGCCGAC CGGTGCGCAT CGCGTGCCAC GGCGCGTTCG ACTACACCCT CAGCACGGCG 
CTGTCGCCGC AGCTGCACAT TGGTGTCGTC GACGCGCCGA AGCGGACGCG CATGCACCTG 
ATCTTGTCTC GCCACATCAC CGACGTCTCG GAGTTTGGCC GGCAGTACGA GAAGGACGAC 
GACACGACAC CGTACCTGGC CTTGAAGGTG TACGACGTGA CCGACTACCC GTCTGTAGCG 
CAGCACCTTG GTGGCCATTG CTCGCTGGAG ATGTGCTACT GCCGCCGCCT CGCCTCGAAT 
TCGGACTTTA AGAATAGCAT TGAGCCCCTC AACGACGTCA TCTACCGCGC CTCGTCTGCG 
CGCACCTTCA GCTTCGACTG TCCTGCCGGC TCGAGCAATC TGGTGGTGGT CGTGTCGCGG 
ACTGCCTCAA AGGCGCGGCA GCCTTTCAAC TTTACGGTGA CCCTGCACAC GGAGCTGGAG 
CAGATGCGGC TGCCGCGGCT GCAGGGCAAC TACGGCAGCG GCGCCGAGGA CAAGAACTCC 
CTCCTGGCAA GCGCCAACGC CAACGCCGGG AACCCGTGCG CTGGAGTGTA CATGCACATG 
ATACCGACCT CCTCGCTCAA GTTCACGACG CGGCTGTCCG GCGCGTGGGT GAAGGGCAAG 
ACTTGCGGCG GCCGCACCGA CGCCGCCACG TATGTCTTCA ACCCGCAGTA CCGCCTGCAC 
CTGGACCAGC CCTCGCACGT GTCGCTGAGG CTGGCTGTGG CAGGATGCGA GGTGCCGTGC 
TCGATTCAGA TGATCAAGCC GATGTCGTCC AACAAATACG AGGAGAACGG CGGCAGCGGT 
GGAATCAAGC GCAAGATGAC TGACTTCGAC GGCCGTGTCG GTAACGGTGC CAAGGCGGGC 
GAGCTTGTTC TCGAGAGCGC CCGCTACGCC TTTGGTGGGG CCGTTCTAGA TAGCGCGCTG 
CCGTTCTGCG TCTGCTACGA CCGCTACCGC GCCCTCGGCA AGCTGCATTG GAGGGAAGCG 
AAGAAGGTGC TGGTGAACGT GACCGCCCCG AACCGGACGC CTGAGTCCTT CATCGTCTAC 
GACCTCGGGG ACACCGACAC CGTGGCGGTG CTTCTCCTGA ATGCTATCAA GAAGGGCTGC 
GCTGCCTCCC CACGTGACGC GCACTTTAGC GTGAACGGGA AGCCGATCAC GCTCGAGGCC 
ACCATCGCGT CGCTCTTCCA GCTCGCATCC TCCCTCGGCG GCGAGTCCCT CGCCTCTGGC 
GGGGATGGCG CGACAACCGC GAAGTCTGTC ACAGTAGCCC TCGCCCTGGA CGGCAAGGCG 
GCGTCGATCG AGTCTGCCTC GAGCCCACCT GTGAGGAAGC TGAAGGAAGT CATGAACGAC 
ATAGTGTCTG ATGTGCTCAA GTGCTCGTCG CAGTTCAGCA GCTACGTGCC ACGCTTGACG 
CATCTTGCCA GGCAGGCAAA CAGCGCGCTG ACCGGTGCCA GCGATGCGGA CGTGCGAGCG 
TACATTCTCG AGCTCGCCGC TTACCTTGCT GACTACGTGA AGCGGTGTGC CAGCGCGGTG 
CGGTCGGCGC AGACGCCGCA GGCGCTGCTT CCGCTGCTTC CTGCCGGCGA CTACACGGTC 
ATTCCATCTT TGTGGGAGAA GGGTAAGCCG GGCAGCTTTG AGCTGACGGT GGAGACGTCA 
CAGCCACATA AGACGTCGGC GATTCCGAAG GAGGGACACA ACATGCAAGA GACGCTTGTG 
CGCGGCGCAC TACGCCCCGT CGCGGTTTGC CTGGGCACGT CACTGATGAA GCCGAGAGCA 
GTGCTCAGCA TGAGGGATCC GTTCTTTGAG AACAGCAAGG TGCAGGTGCT GTGCCCCGCC 
CGCTCCGTCT TCATGAGTCG TCTCTTTGTC CTGGTTGACA TGGTGGATGC GGAGGCGAAG 
GGCCAGCCGG CCAAGGCCCC CGCCACGAAT CTGAGCCTCT TCCGTGTGCA CAACGCGTCA 
TCGATGGAGC TGGTGTGCGC TTCAGACGAG TACTCCCATG GCGGTGTGTC AACCCCACCT 
GTAGAGCTGG AGGCGAAGAC GCAGTATCTG CTCGTGGTGA GCGCAAAGGA GAGCGTGGCT 
GACAAGTTCC TCCTCCGCAT CTATACCAGC GGCAGCTGCA CCGCTCAGCT GGCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_33_2130 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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