LINJ_28_0940 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_28_0940
Entrez Gene ID	5070309
Full Name	putative minichromosome maintenance (MCM) complex subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001470087.1	XP_001470124.1	putative minichromosome maintenance (MCM) complex subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_28_0940 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_28_0940 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc03892	XM_001470087.1 Latest version!	Leishmania infantum JPCM5 putative minichromosome maintenance (MCM) complex subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_28_0940 ( XM_001470087.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001470124.1 Leishmania infantum JPCM5 putative minichromosome maintenance (MCM) complex subunit partial mRNA.

Price & Availability↑

CloneID	OLc03892
Clone ID Related Accession (Same CDS sequence)	XM_001470087.1
Accession Version	XM_001470087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative minichromosome maintenance (MCM) complex subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009412). COMPLETENESS: incomplete on both ends.

ATGCGACCCT CGAGAGACCG GGACGCCGCT CGCCGCAAAC GGCGCCGCGC GCGAGAGGAC 
GAGGTCCATG CCGCAGGCCC GCACCCACCG AGGCGAGAGG AGGTCGAGGA CGACGATGAA 
GCGGACCACT CCTCCGGTGA CGAGGCCAAC GACGAGGAGG GGGAAGACTT GTACGGGGAG 
AACTTCATGC AGGACTATCT CCAGCCGGAT GAGGAGAGCG AGGTGGCCGA GGAGGAAGTC 
GGCGAGGACA ACGACTGGAT TGCAGACGAC GACAGCTCCG TTTCCGAGAT CTCCGAGGGC 
GGCCGCATCG CTGTGGATGA GCTGCTCGAG CGCCGTCGGG AGAAAGAGGA GGCGCTAGCC 
GAGGAGCGGA GGCAGCTGCA GGAAGGCATC TTCAGCGACG TGGACGACGA CGATGGCGAC 
GATGACGATG ATGCGTCGTG GGCTTCCGAA GAGGGCGCGG GCGGTTTGAC ACGGAGTGGG 
GGCGCCGCGG TTGGCGATCA AGGCAGCGAC ATCGATCACG GAGAGGACGT CGGCTACGGC 
GATCCCAACG ACGAAGCCTA CGTGCGTGGA GACCTGGAGT CGATGAACTT CAACTGGCGG 
CAGCCGCAGG GGGAGCTGGT GGAGTGGCTT GCGCAGGAGC TGCCTCGCCG CGTGATTAAG 
AACCGCATCT ACAACTTCTA CTACAACTAC ATCGTGAACG ACGTCAGCGT CTACGAGGAG 
AAGGTGAACG CAATGACACG CGAAAACGAC AAGAGTTTTC AGCTGAGCTA CGATCATCTG 
AGCCGCGTGT ACGACAGTGT TCTCGCGCTG TGGCTGGTGG ACGCTCCAGA TCCGATGATC 
GAGCTACTGG AGGAGGCTGC CAACTACTTC ACCTTCAAGC TATACCCGCA GTATCGCAAG 
GTGCACTCGC ACATCTTTGT TCGGATTTGC GACCTGCCCT TGTGCGATCC GATCCGCGAC 
TTTCGCCAGG TGCACATGAA TGTGCTCGTA CGGGTGGAGG GGGTGGTGAT TCGCCGCTCA 
CCGGTGTACC CGCAGATGGA CGCCGTCAAG TACGACTGCG CGCGGTGCTC CTACATTATC 
GGTCCCATCT ACCAGCGCGG CGACAAGGAG CAGCGCGTGA GCATGTGCCC CAGCTGCCAC 
AGCAAGGGGC CGTTCCGCGT GAACATGCGC CTGACGGAGT ACCGCAACCA TCAAACGATA 
GTTCTGCAGG AGCCCCCTGG CAAGGTACCG CCTGGGCGGC TGCCGCGCAG CTTGGAGGTG 
GTACTGACGA ACGACCTCAT TGACCGCGCG AAACCTGGCG AAGAGGTGGA CGTGACGGGG 
ATATACCGCA ACAACTTCGA TCCGTTGCTC AACAGCCGCC AAGGCTTTCC CGTCTTCACG 
ACCGTGCTGC ACGCGAACAA CGTTATCCGA CGCACTACGG AGCTGGGCAT GTTTCGGCTG 
CCAGATGATG AACGACAACG CATTATAGAG CTCAGTAAGT CGCCCAACAT TCGAAAGAAG 
CTCTTACAGT CCATTGCGCC GAGCATCCAC GGCCGCGACG ACATCAAGCT CGGGCTGCTG 
CTGGCCATGA TGGGTGCAGT GCCAAAGGAC ATCGGCGGCG ACCAGTCGCA TCGCATTCGG 
GGCGACATCA ACGTCCTCAT GGTTGGCGAT CCCGGCTGCG CAAAGTCGCA GTTTCTCAAG 
TTTGTTGAGA AAACGGCCGA CCGCACAGTC TTCACGACTG GCCGCGGCTC CACCGCTGTT 
GGTCTGACGG CATCTGTGCA CAAGGACAGC GTCAATGGCG ACTTCGTGCT GGAGGGCGGC 
GCCCTCGTCA TTGCGGACCG CGGCTGCTGC CTCATTGACG AGTTCGACAA AATGTCTGAC 
CAAGATCGAA CGTCCATCCA CGAGGCTATG GAACAGCAGA CGATCTCGGT GGCGCGCGGT 
GGCATTGTAA CGACGCTGTC CGCGCGCTGC TGCATCATCG CGGCGGCGAA TCCAATGGGC 
GGCCGCTACG ACCCTTCTAC CTCGTTTGAT GCCAACGTGA ATCTTACCAC ACCGATCCTC 
TCCCGCTTCG ACTTGCTGTT TGTGGTGCGG GATGAGGTGA ACGTCGAGTT GGACGAGCGG 
CTTGCGACGT TTATCTGCGA CTCGCACATG CGCAACCACC CGCGAACGCA GGAGGAGACG 
CGGCTTCTGC AGCGCGACAG GCACGAGGAG CTGTCGAGGT TGCGCTACGC GCTGGAGAAC 
GCGTCAACAG AGGGCGAGCG CGAGGAGTGC GAGGAGCAGC TGCGGCGCCT GCGCGAGAGC 
GTCGAGGATT CTTCCCGCTT CGAGGACGAC GACCCAGACA GCGACAAGCC GCTCCCGCAA 
TCCTTGCTGC GCAAGTACAT CCTGTTCGCG AAGAGTCACT GCTTTCCCCG CATCTCCAAC 
ATCGACCCCG ATACCATTGC ACGTCTATAT GTCGAGCTGC GGCAGGAGTC GAAGCACGGC 
GGCATCGCCA TCACAGTGCG GCACATGGAA TCCGTCATTC GCCTCTCCGA GGCGCACGCA 
CGGGTGCATC TTCGGGAATA CGTGACCGAC GAGGACGTCA CCGCGGCCGT CTCGCTCTTT 
CTCCGCTGCT TCATACAGAC ACAGAAGTAC AGTCTACGCA GCGCGATGGA GGCGCGCTTC 
CGCAAGTTCC TCGAATCCGA CACGGAAAGT CTACCACTCA TTCGCCACCG CATCAAGGTG 
GCGGTGCAGA CGGTGCGCCA GTTCGAGCGC CAGCTGTCTG GTGGGGTGGA GCCAACCCAA 
GTGCGCATCG ACGTATCGGA GCTGGACTAC TACACAGCGA ACATGTCTCA AGAGGCCCTG 
AACGCCTTCT ATGAGAGTGC GGAGTTCAGG AAGGAGTACC GGCTGATTCG CGACCCCGTG 
ACGCACGCGC CTCTGCAGAT TGAGCACTCG CTGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001470124.1
CDS	1..2919
Translation	MRPSRDRDAARRKRRRAREDEVHAAGPHPPRREEVEDDDEADHSSGDEANDEEGEDLYGENFMQDYLQPDEESEVAEEEVGEDNDWIADDDSSVSEISEGGRIAVDELLERRREKEEALAEERRQLQEGIFSDVDDDDGDDDDDASWASEEGAGGLTRSGGAAVGDQGSDIDHGEDVGYGDPNDEAYVRGDLESMNFNWRQPQGELVEWLAQELPRRVIKNRIYNFYYNYIVNDVSVYEEKVNAMTRENDKSFQLSYDHLSRVYDSVLALWLVDAPDPMIELLEEAANYFTFKLYPQYRKVHSHIFVRICDLPLCDPIRDFRQVHMNVLVRVEGVVIRRSPVYPQMDAVKYDCARCSYIIGPIYQRGDKEQRVSMCPSCHSKGPFRVNMRLTEYRNHQTIVLQEPPGKVPPGRLPRSLEVVLTNDLIDRAKPGEEVDVTGIYRNNFDPLLNSRQGFPVFTTVLHANNVIRRTTELGMFRLPDDERQRIIELSKSPNIRKKLLQSIAPSIHGRDDIKLGLLLAMMGAVPKDIGGDQSHRIRGDINVLMVGDPGCAKSQFLKFVEKTADRTVFTTGRGSTAVGLTASVHKDSVNGDFVLEGGALVIADRGCCLIDEFDKMSDQDRTSIHEAMEQQTISVARGGIVTTLSARCCIIAAANPMGGRYDPSTSFDANVNLTTPILSRFDLLFVVRDEVNVELDERLATFICDSHMRNHPRTQEETRLLQRDRHEELSRLRYALENASTEGEREECEEQLRRLRESVEDSSRFEDDDPDSDKPLPQSLLRKYILFAKSHCFPRISNIDPDTIARLYVELRQESKHGGIAITVRHMESVIRLSEAHARVHLREYVTDEDVTAAVSLFLRCFIQTQKYSLRSAMEARFRKFLESDTESLPLIRHRIKVAVQTVRQFERQLSGGVEPTQVRIDVSELDYYTANMSQEALNAFYESAEFRKEYRLIRDPVTHAPLQIEHSLA

Target ORF information:

RefSeq Version	XM_001470087.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative minichromosome maintenance (MCM) complex subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001470087.1

ORF Insert Sequence:

ATGCGACCCT CGAGAGACCG GGACGCCGCT CGCCGCAAAC GGCGCCGCGC GCGAGAGGAC 
GAGGTCCATG CCGCAGGCCC GCACCCACCG AGGCGAGAGG AGGTCGAGGA CGACGATGAA 
GCGGACCACT CCTCCGGTGA CGAGGCCAAC GACGAGGAGG GGGAAGACTT GTACGGGGAG 
AACTTCATGC AGGACTATCT CCAGCCGGAT GAGGAGAGCG AGGTGGCCGA GGAGGAAGTC 
GGCGAGGACA ACGACTGGAT TGCAGACGAC GACAGCTCCG TTTCCGAGAT CTCCGAGGGC 
GGCCGCATCG CTGTGGATGA GCTGCTCGAG CGCCGTCGGG AGAAAGAGGA GGCGCTAGCC 
GAGGAGCGGA GGCAGCTGCA GGAAGGCATC TTCAGCGACG TGGACGACGA CGATGGCGAC 
GATGACGATG ATGCGTCGTG GGCTTCCGAA GAGGGCGCGG GCGGTTTGAC ACGGAGTGGG 
GGCGCCGCGG TTGGCGATCA AGGCAGCGAC ATCGATCACG GAGAGGACGT CGGCTACGGC 
GATCCCAACG ACGAAGCCTA CGTGCGTGGA GACCTGGAGT CGATGAACTT CAACTGGCGG 
CAGCCGCAGG GGGAGCTGGT GGAGTGGCTT GCGCAGGAGC TGCCTCGCCG CGTGATTAAG 
AACCGCATCT ACAACTTCTA CTACAACTAC ATCGTGAACG ACGTCAGCGT CTACGAGGAG 
AAGGTGAACG CAATGACACG CGAAAACGAC AAGAGTTTTC AGCTGAGCTA CGATCATCTG 
AGCCGCGTGT ACGACAGTGT TCTCGCGCTG TGGCTGGTGG ACGCTCCAGA TCCGATGATC 
GAGCTACTGG AGGAGGCTGC CAACTACTTC ACCTTCAAGC TATACCCGCA GTATCGCAAG 
GTGCACTCGC ACATCTTTGT TCGGATTTGC GACCTGCCCT TGTGCGATCC GATCCGCGAC 
TTTCGCCAGG TGCACATGAA TGTGCTCGTA CGGGTGGAGG GGGTGGTGAT TCGCCGCTCA 
CCGGTGTACC CGCAGATGGA CGCCGTCAAG TACGACTGCG CGCGGTGCTC CTACATTATC 
GGTCCCATCT ACCAGCGCGG CGACAAGGAG CAGCGCGTGA GCATGTGCCC CAGCTGCCAC 
AGCAAGGGGC CGTTCCGCGT GAACATGCGC CTGACGGAGT ACCGCAACCA TCAAACGATA 
GTTCTGCAGG AGCCCCCTGG CAAGGTACCG CCTGGGCGGC TGCCGCGCAG CTTGGAGGTG 
GTACTGACGA ACGACCTCAT TGACCGCGCG AAACCTGGCG AAGAGGTGGA CGTGACGGGG 
ATATACCGCA ACAACTTCGA TCCGTTGCTC AACAGCCGCC AAGGCTTTCC CGTCTTCACG 
ACCGTGCTGC ACGCGAACAA CGTTATCCGA CGCACTACGG AGCTGGGCAT GTTTCGGCTG 
CCAGATGATG AACGACAACG CATTATAGAG CTCAGTAAGT CGCCCAACAT TCGAAAGAAG 
CTCTTACAGT CCATTGCGCC GAGCATCCAC GGCCGCGACG ACATCAAGCT CGGGCTGCTG 
CTGGCCATGA TGGGTGCAGT GCCAAAGGAC ATCGGCGGCG ACCAGTCGCA TCGCATTCGG 
GGCGACATCA ACGTCCTCAT GGTTGGCGAT CCCGGCTGCG CAAAGTCGCA GTTTCTCAAG 
TTTGTTGAGA AAACGGCCGA CCGCACAGTC TTCACGACTG GCCGCGGCTC CACCGCTGTT 
GGTCTGACGG CATCTGTGCA CAAGGACAGC GTCAATGGCG ACTTCGTGCT GGAGGGCGGC 
GCCCTCGTCA TTGCGGACCG CGGCTGCTGC CTCATTGACG AGTTCGACAA AATGTCTGAC 
CAAGATCGAA CGTCCATCCA CGAGGCTATG GAACAGCAGA CGATCTCGGT GGCGCGCGGT 
GGCATTGTAA CGACGCTGTC CGCGCGCTGC TGCATCATCG CGGCGGCGAA TCCAATGGGC 
GGCCGCTACG ACCCTTCTAC CTCGTTTGAT GCCAACGTGA ATCTTACCAC ACCGATCCTC 
TCCCGCTTCG ACTTGCTGTT TGTGGTGCGG GATGAGGTGA ACGTCGAGTT GGACGAGCGG 
CTTGCGACGT TTATCTGCGA CTCGCACATG CGCAACCACC CGCGAACGCA GGAGGAGACG 
CGGCTTCTGC AGCGCGACAG GCACGAGGAG CTGTCGAGGT TGCGCTACGC GCTGGAGAAC 
GCGTCAACAG AGGGCGAGCG CGAGGAGTGC GAGGAGCAGC TGCGGCGCCT GCGCGAGAGC 
GTCGAGGATT CTTCCCGCTT CGAGGACGAC GACCCAGACA GCGACAAGCC GCTCCCGCAA 
TCCTTGCTGC GCAAGTACAT CCTGTTCGCG AAGAGTCACT GCTTTCCCCG CATCTCCAAC 
ATCGACCCCG ATACCATTGC ACGTCTATAT GTCGAGCTGC GGCAGGAGTC GAAGCACGGC 
GGCATCGCCA TCACAGTGCG GCACATGGAA TCCGTCATTC GCCTCTCCGA GGCGCACGCA 
CGGGTGCATC TTCGGGAATA CGTGACCGAC GAGGACGTCA CCGCGGCCGT CTCGCTCTTT 
CTCCGCTGCT TCATACAGAC ACAGAAGTAC AGTCTACGCA GCGCGATGGA GGCGCGCTTC 
CGCAAGTTCC TCGAATCCGA CACGGAAAGT CTACCACTCA TTCGCCACCG CATCAAGGTG 
GCGGTGCAGA CGGTGCGCCA GTTCGAGCGC CAGCTGTCTG GTGGGGTGGA GCCAACCCAA 
GTGCGCATCG ACGTATCGGA GCTGGACTAC TACACAGCGA ACATGTCTCA AGAGGCCCTG 
AACGCCTTCT ATGAGAGTGC GGAGTTCAGG AAGGAGTACC GGCTGATTCG CGACCCCGTG 
ACGCACGCGC CTCTGCAGAT TGAGCACTCG CTGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_28_0940 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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