Kif21a cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif21a
Entrez Gene ID	101974311
Full Name	kinesin family member 21A
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005334026.1	XP_005334083.1	kinesin-like protein KIF21A isoform X1
XM_005334027.2	XP_005334084.1	kinesin-like protein KIF21A isoform X3
XM_021732221.1	XP_021587896.1	kinesin-like protein KIF21A isoform X4
XM_005334026.2	XP_005334083.1	kinesin-like protein KIF21A isoform X1
XM_005334027.1	XP_005334084.1	kinesin-like protein KIF21A isoform X2
XM_021732222.1	XP_021587897.1	kinesin-like protein KIF21A isoform X5
XM_021732220.1	XP_021587895.1	kinesin-like protein KIF21A isoform X2

Pathways from BioSystems
Gene Ontology

The following Kif21a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif21a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc64912	XM_005334026.2 Latest version!	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc64913	XM_005334027.2 Latest version!	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc82055	XM_021732220.1 Latest version!	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc82056	XM_021732221.1 Latest version!	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc82057	XM_021732222.1 Latest version!	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OIc64912	XM_005334026.1	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc64913	XM_005334027.1	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif21a ( XM_005334026.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005334083.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

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CloneID	OIc64912
Clone ID Related Accession (Same CDS sequence)	XM_005334026.1 , XM_005334026.2
Accession Version	XM_005334026.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005334083.1
CDS	414..5438
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESNREERSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_005334026.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005334026.1

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_005334026.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005334083.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

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CloneID	OIc64912
Clone ID Related Accession (Same CDS sequence)	XM_005334026.1 , XM_005334026.2
Accession Version	XM_005334026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005334026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005334083.1
CDS	417..5441
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESNREERSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_005334026.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005334026.2

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_005334027.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005334084.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIc64913
Clone ID Related Accession (Same CDS sequence)	XM_005334027.2 , XM_005334027.1
Accession Version	XM_005334027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005334027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG ATCTAGATAG CGTACCATTA 
GAAAATGTAG AGGATAGTAC TGATGAAGAT GCACCTTTAA ACAGCCCAGG ATCAGAAGGA 
AGCACACTCT CTTCAGACCT TATGAAGCTT TGTGGTGAAG TGAAACCTAA GAACAAGGCC 
CGAAGGAGAA CCACCACTCA GATGGAATTG CTGTATGCAG ATAGCAGTGA ACTAGCTTCA 
GACACTAGTG CAGGAGATGC CTCCTTGCCT GGCCCTCTTA CACCTGTTGC AGAAGGGCAA 
GAGATTGGAA TGAATACAGA GACCAGTGGT ACTTCTGCTA GGGATAAAGA GCTTCTTCCC 
CCATCTGGCT TACCTTCTAA GATAGGCAGC ATTTCTAGAC AGTCATCTCT GTCAGAAAAG 
AAATTACCAG AGCCTTCCCC TGTAACACGG AGAAAGGCAT ATGAAAAAGC AGAAAAATCA 
AAGTCCAAGG AACAAAAACA CTCAGATTCT GGAACTTCAG AAGCTAGTCT CTCACCTCCT 
TCTTCCCCAC CAAGCCGGCC CCGTAATGAA CTGAATGTCT TTAATCGTCT TACTGTTTCT 
CAGGGAAACA CCTCAGTTCA GCAGGATAAG TCTGATGAAA GTGACTCCTC TCTGTCGGAG 
GTACACAGAT CCTCCAAAAG GGGCATAATC AACCCATTTC CTGCTTCGAA AGGAATCAGA 
GCATCTCCAC TTCAGTGTAT TCACATAGCT GAAGGGCATA CAAAAGCTGT GCTCTGTGTG 
GATTCTACTG ATGATCTCCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTATGGAAT 
CTGGTCACTG GGCAAGAGAT AATGTCACTG GGGGGTCATC CCAACAACGT TGTATCTGTA 
AAATACTGTA ATTATACCAG TTTGGTCTTC ACTGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG ATTCAGCAAA GTGCATCCGA ACACTGACGT CGTCAGGTCA AGTTACCCTT 
GGAGATGCTT GTTCTGCAAG TACCAGCCGG ACAGTGGCTA TTCCTTCTGG GGAGAACCAG 
ATCAATCAAA TTGCACTAAA TCCATCCGGC ACATTCCTCT ATGCAGCTTC TGGAAATGCT 
GTCAGAATGT GGGATCTTAA AAGGTTTCAG TCTACAGGAA AGTTAACAGG ACACCTAGGC 
CCTGTTATGT GCCTTACTGT AGAACAGATT TCCAGTGGAC AAGATCTAAT CATCACTGGC 
TCTAAGGATC ATTACATCAA AATGTTTGAT GTAACTGAAG GGGCTCTTGG AACTGTGAGT 
CCCACCCACA ATTTTGAACC CCCTCATTAT GATGGCATAG AAGCGCTAAC CATTCAAGGG 
GATAATCTAT TTAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAAAAA 
GACCTTCTTC AGCAAGTTCC TAATGCTCAT AAAGATTGGG TCTGTGCCCT GGGAGTGGTG 
CCAGGCCACC CACTTTTGCT CAGTGGCTGC AGAGGGGGCA TTTTGAAGCT CTGGAACATG 
GATACTTTTG TGCCAGCTGG AGAGATGAAG GGTCATGATA GTCCTATCAA TGCCATATGT 
GTTAATTCTA CCCACATTTT TACTGCAGCT GATGACCGAA CTGTGAGAAT TTGGAAGGCT 
CGCAATTTGC AAGATGGTCA GATCTCTGAC ACAGGAGATA TGGGGGAAGA TTTTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005334084.1
CDS	416..5401
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_005334027.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005334027.2

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG ATCTAGATAG CGTACCATTA 
GAAAATGTAG AGGATAGTAC TGATGAAGAT GCACCTTTAA ACAGCCCAGG ATCAGAAGGA 
AGCACACTCT CTTCAGACCT TATGAAGCTT TGTGGTGAAG TGAAACCTAA GAACAAGGCC 
CGAAGGAGAA CCACCACTCA GATGGAATTG CTGTATGCAG ATAGCAGTGA ACTAGCTTCA 
GACACTAGTG CAGGAGATGC CTCCTTGCCT GGCCCTCTTA CACCTGTTGC AGAAGGGCAA 
GAGATTGGAA TGAATACAGA GACCAGTGGT ACTTCTGCTA GGGATAAAGA GCTTCTTCCC 
CCATCTGGCT TACCTTCTAA GATAGGCAGC ATTTCTAGAC AGTCATCTCT GTCAGAAAAG 
AAATTACCAG AGCCTTCCCC TGTAACACGG AGAAAGGCAT ATGAAAAAGC AGAAAAATCA 
AAGTCCAAGG AACAAAAACA CTCAGATTCT GGAACTTCAG AAGCTAGTCT CTCACCTCCT 
TCTTCCCCAC CAAGCCGGCC CCGTAATGAA CTGAATGTCT TTAATCGTCT TACTGTTTCT 
CAGGGAAACA CCTCAGTTCA GCAGGATAAG TCTGATGAAA GTGACTCCTC TCTGTCGGAG 
GTACACAGAT CCTCCAAAAG GGGCATAATC AACCCATTTC CTGCTTCGAA AGGAATCAGA 
GCATCTCCAC TTCAGTGTAT TCACATAGCT GAAGGGCATA CAAAAGCTGT GCTCTGTGTG 
GATTCTACTG ATGATCTCCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTATGGAAT 
CTGGTCACTG GGCAAGAGAT AATGTCACTG GGGGGTCATC CCAACAACGT TGTATCTGTA 
AAATACTGTA ATTATACCAG TTTGGTCTTC ACTGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG ATTCAGCAAA GTGCATCCGA ACACTGACGT CGTCAGGTCA AGTTACCCTT 
GGAGATGCTT GTTCTGCAAG TACCAGCCGG ACAGTGGCTA TTCCTTCTGG GGAGAACCAG 
ATCAATCAAA TTGCACTAAA TCCATCCGGC ACATTCCTCT ATGCAGCTTC TGGAAATGCT 
GTCAGAATGT GGGATCTTAA AAGGTTTCAG TCTACAGGAA AGTTAACAGG ACACCTAGGC 
CCTGTTATGT GCCTTACTGT AGAACAGATT TCCAGTGGAC AAGATCTAAT CATCACTGGC 
TCTAAGGATC ATTACATCAA AATGTTTGAT GTAACTGAAG GGGCTCTTGG AACTGTGAGT 
CCCACCCACA ATTTTGAACC CCCTCATTAT GATGGCATAG AAGCGCTAAC CATTCAAGGG 
GATAATCTAT TTAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAAAAA 
GACCTTCTTC AGCAAGTTCC TAATGCTCAT AAAGATTGGG TCTGTGCCCT GGGAGTGGTG 
CCAGGCCACC CACTTTTGCT CAGTGGCTGC AGAGGGGGCA TTTTGAAGCT CTGGAACATG 
GATACTTTTG TGCCAGCTGG AGAGATGAAG GGTCATGATA GTCCTATCAA TGCCATATGT 
GTTAATTCTA CCCACATTTT TACTGCAGCT GATGACCGAA CTGTGAGAAT TTGGAAGGCT 
CGCAATTTGC AAGATGGTCA GATCTCTGAC ACAGGAGATA TGGGGGAAGA TTTTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_005334027.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005334084.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

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CloneID	OIc64913
Clone ID Related Accession (Same CDS sequence)	XM_005334027.2 , XM_005334027.1
Accession Version	XM_005334027.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG ATCTAGATAG CGTACCATTA 
GAAAATGTAG AGGATAGTAC TGATGAAGAT GCACCTTTAA ACAGCCCAGG ATCAGAAGGA 
AGCACACTCT CTTCAGACCT TATGAAGCTT TGTGGTGAAG TGAAACCTAA GAACAAGGCC 
CGAAGGAGAA CCACCACTCA GATGGAATTG CTGTATGCAG ATAGCAGTGA ACTAGCTTCA 
GACACTAGTG CAGGAGATGC CTCCTTGCCT GGCCCTCTTA CACCTGTTGC AGAAGGGCAA 
GAGATTGGAA TGAATACAGA GACCAGTGGT ACTTCTGCTA GGGATAAAGA GCTTCTTCCC 
CCATCTGGCT TACCTTCTAA GATAGGCAGC ATTTCTAGAC AGTCATCTCT GTCAGAAAAG 
AAATTACCAG AGCCTTCCCC TGTAACACGG AGAAAGGCAT ATGAAAAAGC AGAAAAATCA 
AAGTCCAAGG AACAAAAACA CTCAGATTCT GGAACTTCAG AAGCTAGTCT CTCACCTCCT 
TCTTCCCCAC CAAGCCGGCC CCGTAATGAA CTGAATGTCT TTAATCGTCT TACTGTTTCT 
CAGGGAAACA CCTCAGTTCA GCAGGATAAG TCTGATGAAA GTGACTCCTC TCTGTCGGAG 
GTACACAGAT CCTCCAAAAG GGGCATAATC AACCCATTTC CTGCTTCGAA AGGAATCAGA 
GCATCTCCAC TTCAGTGTAT TCACATAGCT GAAGGGCATA CAAAAGCTGT GCTCTGTGTG 
GATTCTACTG ATGATCTCCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTATGGAAT 
CTGGTCACTG GGCAAGAGAT AATGTCACTG GGGGGTCATC CCAACAACGT TGTATCTGTA 
AAATACTGTA ATTATACCAG TTTGGTCTTC ACTGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG ATTCAGCAAA GTGCATCCGA ACACTGACGT CGTCAGGTCA AGTTACCCTT 
GGAGATGCTT GTTCTGCAAG TACCAGCCGG ACAGTGGCTA TTCCTTCTGG GGAGAACCAG 
ATCAATCAAA TTGCACTAAA TCCATCCGGC ACATTCCTCT ATGCAGCTTC TGGAAATGCT 
GTCAGAATGT GGGATCTTAA AAGGTTTCAG TCTACAGGAA AGTTAACAGG ACACCTAGGC 
CCTGTTATGT GCCTTACTGT AGAACAGATT TCCAGTGGAC AAGATCTAAT CATCACTGGC 
TCTAAGGATC ATTACATCAA AATGTTTGAT GTAACTGAAG GGGCTCTTGG AACTGTGAGT 
CCCACCCACA ATTTTGAACC CCCTCATTAT GATGGCATAG AAGCGCTAAC CATTCAAGGG 
GATAATCTAT TTAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAAAAA 
GACCTTCTTC AGCAAGTTCC TAATGCTCAT AAAGATTGGG TCTGTGCCCT GGGAGTGGTG 
CCAGGCCACC CACTTTTGCT CAGTGGCTGC AGAGGGGGCA TTTTGAAGCT CTGGAACATG 
GATACTTTTG TGCCAGCTGG AGAGATGAAG GGTCATGATA GTCCTATCAA TGCCATATGT 
GTTAATTCTA CCCACATTTT TACTGCAGCT GATGACCGAA CTGTGAGAAT TTGGAAGGCT 
CGCAATTTGC AAGATGGTCA GATCTCTGAC ACAGGAGATA TGGGGGAAGA TTTTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005334084.1
CDS	414..5399
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_005334027.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005334027.1

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG ATCTAGATAG CGTACCATTA 
GAAAATGTAG AGGATAGTAC TGATGAAGAT GCACCTTTAA ACAGCCCAGG ATCAGAAGGA 
AGCACACTCT CTTCAGACCT TATGAAGCTT TGTGGTGAAG TGAAACCTAA GAACAAGGCC 
CGAAGGAGAA CCACCACTCA GATGGAATTG CTGTATGCAG ATAGCAGTGA ACTAGCTTCA 
GACACTAGTG CAGGAGATGC CTCCTTGCCT GGCCCTCTTA CACCTGTTGC AGAAGGGCAA 
GAGATTGGAA TGAATACAGA GACCAGTGGT ACTTCTGCTA GGGATAAAGA GCTTCTTCCC 
CCATCTGGCT TACCTTCTAA GATAGGCAGC ATTTCTAGAC AGTCATCTCT GTCAGAAAAG 
AAATTACCAG AGCCTTCCCC TGTAACACGG AGAAAGGCAT ATGAAAAAGC AGAAAAATCA 
AAGTCCAAGG AACAAAAACA CTCAGATTCT GGAACTTCAG AAGCTAGTCT CTCACCTCCT 
TCTTCCCCAC CAAGCCGGCC CCGTAATGAA CTGAATGTCT TTAATCGTCT TACTGTTTCT 
CAGGGAAACA CCTCAGTTCA GCAGGATAAG TCTGATGAAA GTGACTCCTC TCTGTCGGAG 
GTACACAGAT CCTCCAAAAG GGGCATAATC AACCCATTTC CTGCTTCGAA AGGAATCAGA 
GCATCTCCAC TTCAGTGTAT TCACATAGCT GAAGGGCATA CAAAAGCTGT GCTCTGTGTG 
GATTCTACTG ATGATCTCCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTATGGAAT 
CTGGTCACTG GGCAAGAGAT AATGTCACTG GGGGGTCATC CCAACAACGT TGTATCTGTA 
AAATACTGTA ATTATACCAG TTTGGTCTTC ACTGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG ATTCAGCAAA GTGCATCCGA ACACTGACGT CGTCAGGTCA AGTTACCCTT 
GGAGATGCTT GTTCTGCAAG TACCAGCCGG ACAGTGGCTA TTCCTTCTGG GGAGAACCAG 
ATCAATCAAA TTGCACTAAA TCCATCCGGC ACATTCCTCT ATGCAGCTTC TGGAAATGCT 
GTCAGAATGT GGGATCTTAA AAGGTTTCAG TCTACAGGAA AGTTAACAGG ACACCTAGGC 
CCTGTTATGT GCCTTACTGT AGAACAGATT TCCAGTGGAC AAGATCTAAT CATCACTGGC 
TCTAAGGATC ATTACATCAA AATGTTTGAT GTAACTGAAG GGGCTCTTGG AACTGTGAGT 
CCCACCCACA ATTTTGAACC CCCTCATTAT GATGGCATAG AAGCGCTAAC CATTCAAGGG 
GATAATCTAT TTAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAAAAA 
GACCTTCTTC AGCAAGTTCC TAATGCTCAT AAAGATTGGG TCTGTGCCCT GGGAGTGGTG 
CCAGGCCACC CACTTTTGCT CAGTGGCTGC AGAGGGGGCA TTTTGAAGCT CTGGAACATG 
GATACTTTTG TGCCAGCTGG AGAGATGAAG GGTCATGATA GTCCTATCAA TGCCATATGT 
GTTAATTCTA CCCACATTTT TACTGCAGCT GATGACCGAA CTGTGAGAAT TTGGAAGGCT 
CGCAATTTGC AAGATGGTCA GATCTCTGAC ACAGGAGATA TGGGGGAAGA TTTTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_021732220.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021587895.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

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CloneID	OIc82055
Clone ID Related Accession (Same CDS sequence)	XM_021732220.1
Accession Version	XM_021732220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA AAATGTAGAG GATAGTACTG ATGAAGATGC ACCTTTAAAC 
AGCCCAGGAT CAGAAGGAAG CACACTCTCT TCAGACCTTA TGAAGCTTTG TGGTGAAGTG 
AAACCTAAGA ACAAGGCCCG AAGGAGAACC ACCACTCAGA TGGAATTGCT GTATGCAGAT 
AGCAGTGAAC TAGCTTCAGA CACTAGTGCA GGAGATGCCT CCTTGCCTGG CCCTCTTACA 
CCTGTTGCAG AAGGGCAAGA GATTGGAATG AATACAGAGA CCAGTGGTAC TTCTGCTAGG 
GATAAAGAGC TTCTTCCCCC ATCTGGCTTA CCTTCTAAGA TAGGCAGCAT TTCTAGACAG 
TCATCTCTGT CAGAAAAGAA ATTACCAGAG CCTTCCCCTG TAACACGGAG AAAGGCATAT 
GAAAAAGCAG AAAAATCAAA GTCCAAGGAA CAAAAACACT CAGATTCTGG AACTTCAGAA 
GCTAGTCTCT CACCTCCTTC TTCCCCACCA AGCCGGCCCC GTAATGAACT GAATGTCTTT 
AATCGTCTTA CTGTTTCTCA GGGAAACACC TCAGTTCAGC AGGATAAGTC TGATGAAAGT 
GACTCCTCTC TGTCGGAGGT ACACAGATCC TCCAAAAGGG GCATAATCAA CCCATTTCCT 
GCTTCGAAAG GAATCAGAGC ATCTCCACTT CAGTGTATTC ACATAGCTGA AGGGCATACA 
AAAGCTGTGC TCTGTGTGGA TTCTACTGAT GATCTCCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TATGGAATCT GGTCACTGGG CAAGAGATAA TGTCACTGGG GGGTCATCCC 
AACAACGTTG TATCTGTAAA ATACTGTAAT TATACCAGTT TGGTCTTCAC TGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAT TCAGCAAAGT GCATCCGAAC ACTGACGTCG 
TCAGGTCAAG TTACCCTTGG AGATGCTTGT TCTGCAAGTA CCAGCCGGAC AGTGGCTATT 
CCTTCTGGGG AGAACCAGAT CAATCAAATT GCACTAAATC CATCCGGCAC ATTCCTCTAT 
GCAGCTTCTG GAAATGCTGT CAGAATGTGG GATCTTAAAA GGTTTCAGTC TACAGGAAAG 
TTAACAGGAC ACCTAGGCCC TGTTATGTGC CTTACTGTAG AACAGATTTC CAGTGGACAA 
GATCTAATCA TCACTGGCTC TAAGGATCAT TACATCAAAA TGTTTGATGT AACTGAAGGG 
GCTCTTGGAA CTGTGAGTCC CACCCACAAT TTTGAACCCC CTCATTATGA TGGCATAGAA 
GCGCTAACCA TTCAAGGGGA TAATCTATTT AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAAAAAGA CCTTCTTCAG CAAGTTCCTA ATGCTCATAA AGATTGGGTC 
TGTGCCCTGG GAGTGGTGCC AGGCCACCCA CTTTTGCTCA GTGGCTGCAG AGGGGGCATT 
TTGAAGCTCT GGAACATGGA TACTTTTGTG CCAGCTGGAG AGATGAAGGG TCATGATAGT 
CCTATCAATG CCATATGTGT TAATTCTACC CACATTTTTA CTGCAGCTGA TGACCGAACT 
GTGAGAATTT GGAAGGCTCG CAATTTGCAA GATGGTCAGA TCTCTGACAC AGGAGATATG 
GGGGAAGATT TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021587895.1
CDS	416..5419
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESNREERSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_021732220.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021732220.1

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA AAATGTAGAG GATAGTACTG ATGAAGATGC ACCTTTAAAC 
AGCCCAGGAT CAGAAGGAAG CACACTCTCT TCAGACCTTA TGAAGCTTTG TGGTGAAGTG 
AAACCTAAGA ACAAGGCCCG AAGGAGAACC ACCACTCAGA TGGAATTGCT GTATGCAGAT 
AGCAGTGAAC TAGCTTCAGA CACTAGTGCA GGAGATGCCT CCTTGCCTGG CCCTCTTACA 
CCTGTTGCAG AAGGGCAAGA GATTGGAATG AATACAGAGA CCAGTGGTAC TTCTGCTAGG 
GATAAAGAGC TTCTTCCCCC ATCTGGCTTA CCTTCTAAGA TAGGCAGCAT TTCTAGACAG 
TCATCTCTGT CAGAAAAGAA ATTACCAGAG CCTTCCCCTG TAACACGGAG AAAGGCATAT 
GAAAAAGCAG AAAAATCAAA GTCCAAGGAA CAAAAACACT CAGATTCTGG AACTTCAGAA 
GCTAGTCTCT CACCTCCTTC TTCCCCACCA AGCCGGCCCC GTAATGAACT GAATGTCTTT 
AATCGTCTTA CTGTTTCTCA GGGAAACACC TCAGTTCAGC AGGATAAGTC TGATGAAAGT 
GACTCCTCTC TGTCGGAGGT ACACAGATCC TCCAAAAGGG GCATAATCAA CCCATTTCCT 
GCTTCGAAAG GAATCAGAGC ATCTCCACTT CAGTGTATTC ACATAGCTGA AGGGCATACA 
AAAGCTGTGC TCTGTGTGGA TTCTACTGAT GATCTCCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TATGGAATCT GGTCACTGGG CAAGAGATAA TGTCACTGGG GGGTCATCCC 
AACAACGTTG TATCTGTAAA ATACTGTAAT TATACCAGTT TGGTCTTCAC TGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAT TCAGCAAAGT GCATCCGAAC ACTGACGTCG 
TCAGGTCAAG TTACCCTTGG AGATGCTTGT TCTGCAAGTA CCAGCCGGAC AGTGGCTATT 
CCTTCTGGGG AGAACCAGAT CAATCAAATT GCACTAAATC CATCCGGCAC ATTCCTCTAT 
GCAGCTTCTG GAAATGCTGT CAGAATGTGG GATCTTAAAA GGTTTCAGTC TACAGGAAAG 
TTAACAGGAC ACCTAGGCCC TGTTATGTGC CTTACTGTAG AACAGATTTC CAGTGGACAA 
GATCTAATCA TCACTGGCTC TAAGGATCAT TACATCAAAA TGTTTGATGT AACTGAAGGG 
GCTCTTGGAA CTGTGAGTCC CACCCACAAT TTTGAACCCC CTCATTATGA TGGCATAGAA 
GCGCTAACCA TTCAAGGGGA TAATCTATTT AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAAAAAGA CCTTCTTCAG CAAGTTCCTA ATGCTCATAA AGATTGGGTC 
TGTGCCCTGG GAGTGGTGCC AGGCCACCCA CTTTTGCTCA GTGGCTGCAG AGGGGGCATT 
TTGAAGCTCT GGAACATGGA TACTTTTGTG CCAGCTGGAG AGATGAAGGG TCATGATAGT 
CCTATCAATG CCATATGTGT TAATTCTACC CACATTTTTA CTGCAGCTGA TGACCGAACT 
GTGAGAATTT GGAAGGCTCG CAATTTGCAA GATGGTCAGA TCTCTGACAC AGGAGATATG 
GGGGAAGATT TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_021732221.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021587896.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

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CloneID	OIc82056
Clone ID Related Accession (Same CDS sequence)	XM_021732221.1
Accession Version	XM_021732221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021587896.1
CDS	416..5380
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_021732221.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021732221.1

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGTGTGGCT 
GGTAAAGAAG ATAACACAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TATTTCAGAA 
AGAGAAAGCA ATGATTTAGA AGTAGAAGAG AGTCAAGAAG TGAGTGAGCA TGAGGATGAG 
GAAGAGGAGG AGGAAGAGGA GGAAGATGAC ATTGAAGGGG GTGAAAGCTC TGATGAATCA 
GATTCTGAAT CAGATGAAAA AGCCAATTAT CAAGCAGACT TAGCAAACAT TACATGTGAA 
ATTGCAATTA AGCAAAAACT TATTGATGAG CTAGAAAATA GCCAGAAAAG ACTGCAGACG 
CTGAAAAGGC AGTATGAAGA GAAGCTAATG ATGCTACAAC ATAAAATCCG GGATACTCAG 
CTTGAAAGAG ACCAAGTACT TCAAAACTTA GGCTCAGTGG AATCTTACTC AGAAGAAAAG 
GCAAAAAAAG TTAAGTCTGA ATATGAAAAG AAACTACAAG CTATGAATAA AGAACTGCAG 
AGACTTCAGA CTGCTCAAAA AGAACATGCA AGGTTGCTTA AAAATCAGTC TCAGTATGAA 
AAGCAATTGA AGAAATTGCA ACAGGATGTG ATGGAAATGA AAAAAACAAA GGTTCGCCTA 
ATGAAACAAA TGAAAGAAGA ACAAGAGAAA GCCAGGCTGA CAGAATCTAG AAGAAACAGA 
GAGATTGCTC AATTGAAAAA GGATCAACGT AAAAGAGATC ACCAACTTAG ACTTCTGGAA 
GCCCAAAAAA GAAACCAGGA GGTGGTTCTT CGCCGCAAAA CTGAAGAGGT TACAGCTCTT 
CGTCGCCAAG TGAGGCCCAT GTCAGATAAA GTGGCTGGGA AAGTCACTCG GAAGCTTAGC 
TCATCTGATG CTCCTGTTCA GGACGCAGGT TCCAGTGCAG CTGCTGTGGA AGCAGATCCA 
TCAAGGGCAG GCACCCAGCA GAAAATGAGA ATTCCTGTGG CAAGGGTCCA GGCCTTACCA 
GCGCCCACAA CAAATGGAAC CAGAAAAAAA TATCTGAGGA AAGGACTGAC TGGCCGAGTG 
TTTACTTCTA AGACAGCTCG CATGAAGTGG CAGCTCTTAG AGCGCAGGGT CACTGACATT 
ATCATGCAGA AAATGACTAT TTCCAACATG GAGGCCGACA TGGACAGACT CCTCAAGCAA 
CGGGAAGAAC TCACAAAAAG ACGAGAAAAA CTTTCAAAAA GAAGAGAAAA GATAGTCAAG 
GAGAATGGAG AGGGTGATAA AAATGTGGTT AACATCAATG AAGAGATGGA ATCACTGACT 
GCTAACATAG ATTATATCAA TGACAGTATT TCTGATTGTC AAGCCAACAT CATGCAGATG 
GAAGAAGCAA AGGAAGAAGG TGAGACTTTG GATGTCACCG CAGTCATTAA TGCTTGCACA 
CTTACAGAAG CCCGATACCT GCTAGATCAC TTCCTGTCAA TGGGCATCAA TAAGGGTCTT 
CAGGCTGCCC AGAAAGAGGC TCAAATTAAA GTTCTTGAAG GTCGACTCAA ACAAACTGAA 
ATAACCAGTG CTACACAAAA CCAGCTCTTA TTTCATATGT TGAAAGAGAA AGCAGAATTA 
AATCCTGAGC TAGATGCTTT ACTAGGCCAT GCTTTACAAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAC 
TCAGATTCTG GAACTTCAGA AGCTAGTCTC TCACCTCCTT CTTCCCCACC AAGCCGGCCC 
CGTAATGAAC TGAATGTCTT TAATCGTCTT ACTGTTTCTC AGGGAAACAC CTCAGTTCAG 
CAGGATAAGT CTGATGAAAG TGACTCCTCT CTGTCGGAGG TACACAGATC CTCCAAAAGG 
GGCATAATCA ACCCATTTCC TGCTTCGAAA GGAATCAGAG CATCTCCACT TCAGTGTATT 
CACATAGCTG AAGGGCATAC AAAAGCTGTG CTCTGTGTGG ATTCTACTGA TGATCTCCTC 
TTCACTGGAT CAAAAGATCG TACTTGTAAA GTATGGAATC TGGTCACTGG GCAAGAGATA 
ATGTCACTGG GGGGTCATCC CAACAACGTT GTATCTGTAA AATACTGTAA TTATACCAGT 
TTGGTCTTCA CTGTGTCAAC ATCTTATATT AAGGTGTGGG ATATCAGAGA TTCAGCAAAG 
TGCATCCGAA CACTGACGTC GTCAGGTCAA GTTACCCTTG GAGATGCTTG TTCTGCAAGT 
ACCAGCCGGA CAGTGGCTAT TCCTTCTGGG GAGAACCAGA TCAATCAAAT TGCACTAAAT 
CCATCCGGCA CATTCCTCTA TGCAGCTTCT GGAAATGCTG TCAGAATGTG GGATCTTAAA 
AGGTTTCAGT CTACAGGAAA GTTAACAGGA CACCTAGGCC CTGTTATGTG CCTTACTGTA 
GAACAGATTT CCAGTGGACA AGATCTAATC ATCACTGGCT CTAAGGATCA TTACATCAAA 
ATGTTTGATG TAACTGAAGG GGCTCTTGGA ACTGTGAGTC CCACCCACAA TTTTGAACCC 
CCTCATTATG ATGGCATAGA AGCGCTAACC ATTCAAGGGG ATAATCTATT TAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAAAAAG ACCTTCTTCA GCAAGTTCCT 
AATGCTCATA AAGATTGGGT CTGTGCCCTG GGAGTGGTGC CAGGCCACCC ACTTTTGCTC 
AGTGGCTGCA GAGGGGGCAT TTTGAAGCTC TGGAACATGG ATACTTTTGT GCCAGCTGGA 
GAGATGAAGG GTCATGATAG TCCTATCAAT GCCATATGTG TTAATTCTAC CCACATTTTT 
ACTGCAGCTG ATGACCGAAC TGTGAGAATT TGGAAGGCTC GCAATTTGCA AGATGGTCAG 
ATCTCTGACA CAGGAGATAT GGGGGAAGAT TTTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_021732222.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021587897.1 Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

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CloneID	OIc82057
Clone ID Related Accession (Same CDS sequence)	XM_021732222.1
Accession Version	XM_021732222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936607.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAG 
TCTGATGAAA GTGACTCCTC TCTGTCGGAG GTACACAGAT CCTCCAAAAG GGGCATAATC 
AACCCATTTC CTGCTTCGAA AGGAATCAGA GCATCTCCAC TTCAGTGTAT TCACATAGCT 
GAAGGGCATA CAAAAGCTGT GCTCTGTGTG GATTCTACTG ATGATCTCCT CTTCACTGGA 
TCAAAAGATC GTACTTGTAA AGTATGGAAT CTGGTCACTG GGCAAGAGAT AATGTCACTG 
GGGGGTCATC CCAACAACGT TGTATCTGTA AAATACTGTA ATTATACCAG TTTGGTCTTC 
ACTGTGTCAA CATCTTATAT TAAGGTGTGG GATATCAGAG ATTCAGCAAA GTGCATCCGA 
ACACTGACGT CGTCAGGTCA AGTTACCCTT GGAGATGCTT GTTCTGCAAG TACCAGCCGG 
ACAGTGGCTA TTCCTTCTGG GGAGAACCAG ATCAATCAAA TTGCACTAAA TCCATCCGGC 
ACATTCCTCT ATGCAGCTTC TGGAAATGCT GTCAGAATGT GGGATCTTAA AAGGTTTCAG 
TCTACAGGAA AGTTAACAGG ACACCTAGGC CCTGTTATGT GCCTTACTGT AGAACAGATT 
TCCAGTGGAC AAGATCTAAT CATCACTGGC TCTAAGGATC ATTACATCAA AATGTTTGAT 
GTAACTGAAG GGGCTCTTGG AACTGTGAGT CCCACCCACA ATTTTGAACC CCCTCATTAT 
GATGGCATAG AAGCGCTAAC CATTCAAGGG GATAATCTAT TTAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAAAAA GACCTTCTTC AGCAAGTTCC TAATGCTCAT 
AAAGATTGGG TCTGTGCCCT GGGAGTGGTG CCAGGCCACC CACTTTTGCT CAGTGGCTGC 
AGAGGGGGCA TTTTGAAGCT CTGGAACATG GATACTTTTG TGCCAGCTGG AGAGATGAAG 
GGTCATGATA GTCCTATCAA TGCCATATGT GTTAATTCTA CCCACATTTT TACTGCAGCT 
GATGACCGAA CTGTGAGAAT TTGGAAGGCT CGCAATTTGC AAGATGGTCA GATCTCTGAC 
ACAGGAGATA TGGGGGAAGA TTTTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021587897.1
CDS	416..5311
Translation	MWGAPDESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNITEGEQGIISRAVKHLFKSIEEKKHTAIKNGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRMCPQVDAENATDNKMISESSQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATSRSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESNREERSVAGKEDNTDTDQEKKEEKGISERESNDLEVEESQEVSEHEDEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKRQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSDAPVQDAGSSAAAVEADPSRAGTQQKMRIPVARVQALPAPTTNGTRKKYLRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMDRLLKQREELTKRREKLSKRREKIVKENGEGDKNVVNINEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEDAPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSAGDASLPGPLTPVAEGQEIGMNTETSGTSARDKELLPPSGLPSKIGSISRQSSLSEKKLPEPSPVTRRKAYEKAEKSKSKEQKQSDESDSSLSEVHRSSKRGIINPFPASKGIRASPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPSGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVEQISSGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPLLLSGCRGGILKLWNMDTFVPAGEMKGHDSPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDTGDMGEDFASN

Target ORF information:

RefSeq Version	XM_021732222.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021732222.1

ORF Insert Sequence:

ATGTGGGGCG CCCCAGACGA GAGCTCCGTG CGGGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG ATGCCATATT TGTACATCTG TCACACCAGG AGAACCTCAG 
GTCTTCCTAG GGAAGGATAA GGCTTTTACT TTTGACTATG TGTTTGACAT TGACTCTCAG 
CAAGAGCAGA TCTACACTCA GTGTATTGAA AAACTAATTG AAGGTTGTTT TGAAGGATAC 
AATGCTACAG TTTTTGCTTA TGGACAAACT GGAGCTGGCA AAACATACAC AATGGGAACA 
GGATTTGATG TTAATATCAC TGAAGGAGAA CAGGGTATTA TTTCTCGTGC TGTGAAACAT 
CTTTTCAAGA GTATTGAAGA AAAAAAACAT ACAGCAATTA AAAATGGGCT TCCGCCTCCA 
GACTTCAAAG TGAATGCCCA ATTCTTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GTGACATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCGACTGGAG GAATTTATAC TGTGGGAGTC ACAACACGTA CTGTGAATAC AGAATCAGAG 
ATGATGCAGT GTTTGAAGTT GGGTGCTTTA TCCCGCACTA CGGCCAGTAC CCAGATGAAT 
GTTCAGAGTT CTCGTTCACA TGCCATTTTT ACCATTCATG TGTGTCAGAC CAGAATGTGT 
CCTCAAGTAG ATGCTGAAAA TGCAACTGAT AATAAGATGA TTTCTGAATC ATCACAGATG 
AATGAATTTG AAACCTTGAC TGCAAAATTC CATTTTGTTG ATCTGGCAGG ATCAGAAAGA 
CTGAAGCGAA CTGGCGCTAC AGGCGAGAGG GCAAAAGAAG GCATTTCTAT CAACTGTGGA 
CTTTTGGCAC TTGGCAATGT AATAAGTGCA TTGGGAGACA AGAGCAAGAG GGCCACCCAT 
GTCCCTTATA GAGATTCTAA GCTGACAAGA TTATTGCAGG ATTCTCTTGG GGGAAATAGC 
CAAACAATCA TGATAGCATG TGTCAGCCCT TCAGACAGAG ATTTTATGGA AACCTTAAAC 
ACTCTGAAAT ATGCCAATCG GGCTAGAAAT ATCAAGAACA AGGTGATGGT TAATCAGGAC 
AGAGCTAGTC AGCAAATCAA TGCTCTCCGT AGTGAGATCA CGCGACTTCA AATGGAGCTC 
ATGGAATATA AGACGGGTAA AAGGATAATT GACGAGGAGG GTGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTACAGACT GAAAATAACA ACCTGCGTGT AAGAATAAAA 
GCCATGCAAG AGACGGTTGA TGCTTTAAGG TCAAGAATCA CACAGCTTGT TAGTGATCAG 
GCCAACCAAG TTCTTGCCAG AGCAGGTGAA GGAAATGAGG AGATCAGTAA TATGATTCAT 
AGCTATATCA AAGAAATTGA AGATCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAT 
GAGAACCTTC GAAAAAACTT GACAAGAGCC ACATCAAGAT CGCCATATTT CAGTGGATCA 
TCAGCTTTCT CTCCCACTAT ATTATCTTCA GACAAAGAAA CTATTGAAAT TATAGATCTA 
GCTAAGAAAG ATTTGGAGAA GCTGAAAAGG AAGGAAAAGA GGAAGAAAAA AAGGCTACAG 
AAACTTGAGG AAAGCAATCG AGAAGAAAGA AGTGTGGCTG GTAAAGAAGA TAACACAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT ATTTCAGAAA GAGAAAGCAA TGATTTAGAA 
GTAGAAGAGA GTCAAGAAGT GAGTGAGCAT GAGGATGAGG AAGAGGAGGA GGAAGAGGAG 
GAAGATGACA TTGAAGGGGG TGAAAGCTCT GATGAATCAG ATTCTGAATC AGATGAAAAA 
GCCAATTATC AAGCAGACTT AGCAAACATT ACATGTGAAA TTGCAATTAA GCAAAAACTT 
ATTGATGAGC TAGAAAATAG CCAGAAAAGA CTGCAGACGC TGAAAAGGCA GTATGAAGAG 
AAGCTAATGA TGCTACAACA TAAAATCCGG GATACTCAGC TTGAAAGAGA CCAAGTACTT 
CAAAACTTAG GCTCAGTGGA ATCTTACTCA GAAGAAAAGG CAAAAAAAGT TAAGTCTGAA 
TATGAAAAGA AACTACAAGC TATGAATAAA GAACTGCAGA GACTTCAGAC TGCTCAAAAA 
GAACATGCAA GGTTGCTTAA AAATCAGTCT CAGTATGAAA AGCAATTGAA GAAATTGCAA 
CAGGATGTGA TGGAAATGAA AAAAACAAAG GTTCGCCTAA TGAAACAAAT GAAAGAAGAA 
CAAGAGAAAG CCAGGCTGAC AGAATCTAGA AGAAACAGAG AGATTGCTCA ATTGAAAAAG 
GATCAACGTA AAAGAGATCA CCAACTTAGA CTTCTGGAAG CCCAAAAAAG AAACCAGGAG 
GTGGTTCTTC GCCGCAAAAC TGAAGAGGTT ACAGCTCTTC GTCGCCAAGT GAGGCCCATG 
TCAGATAAAG TGGCTGGGAA AGTCACTCGG AAGCTTAGCT CATCTGATGC TCCTGTTCAG 
GACGCAGGTT CCAGTGCAGC TGCTGTGGAA GCAGATCCAT CAAGGGCAGG CACCCAGCAG 
AAAATGAGAA TTCCTGTGGC AAGGGTCCAG GCCTTACCAG CGCCCACAAC AAATGGAACC 
AGAAAAAAAT ATCTGAGGAA AGGACTGACT GGCCGAGTGT TTACTTCTAA GACAGCTCGC 
ATGAAGTGGC AGCTCTTAGA GCGCAGGGTC ACTGACATTA TCATGCAGAA AATGACTATT 
TCCAACATGG AGGCCGACAT GGACAGACTC CTCAAGCAAC GGGAAGAACT CACAAAAAGA 
CGAGAAAAAC TTTCAAAAAG AAGAGAAAAG ATAGTCAAGG AGAATGGAGA GGGTGATAAA 
AATGTGGTTA ACATCAATGA AGAGATGGAA TCACTGACTG CTAACATAGA TTATATCAAT 
GACAGTATTT CTGATTGTCA AGCCAACATC ATGCAGATGG AAGAAGCAAA GGAAGAAGGT 
GAGACTTTGG ATGTCACCGC AGTCATTAAT GCTTGCACAC TTACAGAAGC CCGATACCTG 
CTAGATCACT TCCTGTCAAT GGGCATCAAT AAGGGTCTTC AGGCTGCCCA GAAAGAGGCT 
CAAATTAAAG TTCTTGAAGG TCGACTCAAA CAAACTGAAA TAACCAGTGC TACACAAAAC 
CAGCTCTTAT TTCATATGTT GAAAGAGAAA GCAGAATTAA ATCCTGAGCT AGATGCTTTA 
CTAGGCCATG CTTTACAAGA TCTAGATAGC GTACCATTAG AAAATGTAGA GGATAGTACT 
GATGAAGATG CACCTTTAAA CAGCCCAGGA TCAGAAGGAA GCACACTCTC TTCAGACCTT 
ATGAAGCTTT GTGGTGAAGT GAAACCTAAG AACAAGGCCC GAAGGAGAAC CACCACTCAG 
ATGGAATTGC TGTATGCAGA TAGCAGTGAA CTAGCTTCAG ACACTAGTGC AGGAGATGCC 
TCCTTGCCTG GCCCTCTTAC ACCTGTTGCA GAAGGGCAAG AGATTGGAAT GAATACAGAG 
ACCAGTGGTA CTTCTGCTAG GGATAAAGAG CTTCTTCCCC CATCTGGCTT ACCTTCTAAG 
ATAGGCAGCA TTTCTAGACA GTCATCTCTG TCAGAAAAGA AATTACCAGA GCCTTCCCCT 
GTAACACGGA GAAAGGCATA TGAAAAAGCA GAAAAATCAA AGTCCAAGGA ACAAAAACAG 
TCTGATGAAA GTGACTCCTC TCTGTCGGAG GTACACAGAT CCTCCAAAAG GGGCATAATC 
AACCCATTTC CTGCTTCGAA AGGAATCAGA GCATCTCCAC TTCAGTGTAT TCACATAGCT 
GAAGGGCATA CAAAAGCTGT GCTCTGTGTG GATTCTACTG ATGATCTCCT CTTCACTGGA 
TCAAAAGATC GTACTTGTAA AGTATGGAAT CTGGTCACTG GGCAAGAGAT AATGTCACTG 
GGGGGTCATC CCAACAACGT TGTATCTGTA AAATACTGTA ATTATACCAG TTTGGTCTTC 
ACTGTGTCAA CATCTTATAT TAAGGTGTGG GATATCAGAG ATTCAGCAAA GTGCATCCGA 
ACACTGACGT CGTCAGGTCA AGTTACCCTT GGAGATGCTT GTTCTGCAAG TACCAGCCGG 
ACAGTGGCTA TTCCTTCTGG GGAGAACCAG ATCAATCAAA TTGCACTAAA TCCATCCGGC 
ACATTCCTCT ATGCAGCTTC TGGAAATGCT GTCAGAATGT GGGATCTTAA AAGGTTTCAG 
TCTACAGGAA AGTTAACAGG ACACCTAGGC CCTGTTATGT GCCTTACTGT AGAACAGATT 
TCCAGTGGAC AAGATCTAAT CATCACTGGC TCTAAGGATC ATTACATCAA AATGTTTGAT 
GTAACTGAAG GGGCTCTTGG AACTGTGAGT CCCACCCACA ATTTTGAACC CCCTCATTAT 
GATGGCATAG AAGCGCTAAC CATTCAAGGG GATAATCTAT TTAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAAAAA GACCTTCTTC AGCAAGTTCC TAATGCTCAT 
AAAGATTGGG TCTGTGCCCT GGGAGTGGTG CCAGGCCACC CACTTTTGCT CAGTGGCTGC 
AGAGGGGGCA TTTTGAAGCT CTGGAACATG GATACTTTTG TGCCAGCTGG AGAGATGAAG 
GGTCATGATA GTCCTATCAA TGCCATATGT GTTAATTCTA CCCACATTTT TACTGCAGCT 
GATGACCGAA CTGTGAGAAT TTGGAAGGCT CGCAATTTGC AAGATGGTCA GATCTCTGAC 
ACAGGAGATA TGGGGGAAGA TTTTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Kif21a cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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