ZGRF1 cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZGRF1
Entrez Gene ID	101974011
Full Name	zinc finger GRF-type containing 1
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021729663.1	XP_021585338.1	protein ZGRF1
XM_013360672.1	XP_013216126.1	LOW QUALITY PROTEIN: protein ZGRF1

Pathways from BioSystems
Gene Ontology

The following ZGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc80316	XM_021729663.1 Latest version!	Ictidomys tridecemlineatus zinc finger GRF-type containing 1 (Zgrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc61116	XM_013360672.1 Latest version!	Ictidomys tridecemlineatus zinc finger, GRF-type containing 1 (Zgrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZGRF1 ( XM_021729663.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_021585338.1 Ictidomys tridecemlineatus zinc finger GRF-type containing 1 (Zgrf1), mRNA.

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CloneID	OIc80316
Clone ID Related Accession (Same CDS sequence)	XM_021729663.1
Accession Version	XM_021729663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	protein ZGRF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936563.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACC AAGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGTAT GTTTGGAAAA TCTATTTCTT AAGTGTCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGCG AACGATACTT AATCACAGTT GAGGAGGTTA AAGTTTCTGG AAGCAAAACT 
ATTAAACAGA ATATTATTAA AGAAGCACCA CAGTCAGATT CAAGGAAGTT CATATCCTCT 
GGCCGGTCTC TTGGATGTCA GCCTGCTGGT TTAAAAAGAA AGTTTCCTGG TTTTAAAGCA 
CCACGTCAGG TCCCAACAAA AGTGATTGTT ACAGAAAATG GTGAATCAGC TGCATCACTT 
GAGTCTAAGG AACCTGGCCA TACTTTCTCT TCTCCATTCA ACAGCATACC TTCTTTGTTT 
TCTACTGTTG GCAAGAAAGA TATAAGTACT ATACCAAGAG ACCTTGAAAA TATTGTTACT 
TATGAGAACA GAGAGAGAAA TAGCATGTCT TTGTCTTCAG TTGTCTCTAC TTCATCCTTC 
ATGATTAACC CAGAAATGCT AGGTGAAAAA AATTATTTTT GTTCTCCTGT CAGTACTGGA 
AGTCAGCATT CAGACTCTTC ACTGAACAAT GAACCCCTAA GAAGAGACAA TTTGGCATCT 
CACTATCCAG GAGTTTCACA AAATATTAGA AGTAAAGCCC AGATATTAGC TCTTTTGAAA 
TCTAAGTCAA CCAATATGCA TAAGGACCTA AATTCTGAGA TGACAAGACA TGTACCTCAG 
GTACAGCCAC AAGGAAATTT AAACATTCCT GTGAACCCAA AGTGCTTAAT TCAACCGGAA 
GCATGTGCTG AGATGAAGAG CACAGAAAAT TTATATGACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC CGTACATCCC 
TCTACTATAG GTGAGAGCGA TGTAAAAAGG GAACCTAAAG CTTGTGAGGA TGATATGAGT 
TTGAATTTGA AAGACCTTTT GGTACAAAAA AGGATGCGGA ACTTTGAAAC GTGTGATGAA 
AAGGGAAATG AAGTCCAGCT AGTAGATGAT ACCTATCAAT CCTGGAATCA GGAAGAAAAA 
GTAGAAATAC TTTCTTTCTG TGAAAGCAGC AGCTTACTGG GTAGTTGTAG CAGTGCAGAT 
AATGATGGGT TATTATCAGA ATCTTACATT CAAGGAAATA ATAAAATACC TGTTAATCAA 
AGTGACAAGA TATGCTTGGA AGGATCAGTT TGCATTGGAG AAAGTGCTCA GGTTGTCAAT 
ACAGGTACAA CACAACAGGA GCATAATCAA CCAGTGCCAT CTCTGCTGGA ATCAGAACAT 
CTACAGATCG AATCTTCTCT AAGTAACAAT TCTCAGATCT CTGATGGCAT TGCAGATGTG 
CTTTCTAAAA GTAGTAGTGA TAGTGAAAGT CTAAATAATG TTCATGAATC TATAAGTAAT 
GTAACAGGAC CATTTTTGGA GATAAATTTT AATCTGAGCA ATTTTGAGAC CAGTGACACA 
GAAGAGGAAT CACAGGAAAA CAAAATTTCC CAGGATTCAG AGAGATGGGT AAAGGAAATT 
TTGTCTCACG ATGGCAATTT ATGTGTTCAA AAGAAAAGTG AGGATACAAG CTGTCAAGAA 
ATTGGCAGTG TATGTTTGCC ACTCTTAACA TCTATTGATA GCAAACCTAC AGAGATATTT 
CCTATTAAAG AGACTGTATC ACAGTTTTGT GATAAAACTT GTGTGGGTTT TGACATGGGG 
CCTTATAAAG ATAGAAACAC TGGAAAAGAA ATAGAGGAAG AATATAGTGA CACATTAAGC 
AATTTTGACT CATCTTTAGA ATGGAATGAT GATGTATATG TATATAATAA AGAAGATGCT 
AATAAATCTA TCAAAAAAAT TGGAATTAAC TATGATTTTG CTTCACTTTC AGATAAGTCA 
AAAGGTATAA ACATGAATTT ACATTTTCCT CAAGTTTTAG ACATATTCAC TAATCAGAGT 
CCTAAAAATG GTGCCCTATT CTCAGAAAAT GTTCAACCTC AGACTTTTAT TTTGAGAAGT 
GACATAGACA AAAATGAGGA GCAGATTTTA CTCCCAGCTT CTAGCAGTGA TGAAAGTATC 
CAACTATTAA ATTCCAATCA GGATCACTGT GAACGTGTTG CACTTGATAG ATCAGATATC 
CAAGATTCTA ATTCTTTGTT TTATCCTCTG GGAAAAAAAC ATTCTATTTT AAAAGACTAT 
GAAGTATATA TTCCTGAATC TGAAGACTTG GGAGGAATTA GAAAGTTACC CCAAGACCAT 
GTTGAAGTTG CAATTGCCAG AAAAAGCAAA CAATCCTGGA ATAATTCTAG AAATTCTTCA 
GAACTTTCTG GGGTAATAAA TAACATTTCT CTTTTAAAGT CACTGTCTGA ACACAGTACA 
GCTTTAGAAA GCTTGGAAAC ACTGAAAAAG AAAAATACTG GCTTTCAACA ACAAAGAATT 
CAGCAGACAT ATGATCCAGA TTGCAGTCCT GAAGCTAGGA AACCATTTGC TATAGGAGTT 
TCAAAAACAA TACCAAAGTC TCCTGATCGG AAGCAGGATT CTCAACAGAT ACTAAAAGAA 
GATGAAGTTG AACCAAGTGA ACCATTTCCG TCTCTGCAGC CTGTTGAGTT TCAAGGACAC 
CAACTGAAAG GATCAGCTAC TAGTGGTGTA ATGGTCAGAG GAAAGAGCTC ACAGCTAGTA 
TACAGCCAGT TTCCAGATAG CATGGAATAT CAAAACTTCA TGCCAGATAC TTGTTTTTTG 
ACACCAGAGT TGCCTAGCTC TTGTATGCAT ACTGACTTCT TGCAGGTGAA GTCACCAGAA 
CAAAAGTTCT CTACATTGAG TCCTGTTTCT ACTTTTTCTT TGAACTCAAG AAATGAAGAT 
GTTCTAAAAT TCTCTGAAGA GTCCCTGAAA GCAAGAACTT TACCTGGAAT GAAAAAAATG 
AGTTCTCTGA AGAATAAAAA CCTTCAACAG CTCCCATTAG AGATAAACCG CTCGGAGCTA 
TGCTTCCCAA GTGGACAAAA AGTAAAATGT GCTTATCTTC CCAAAAGGCA GATTCACATA 
CCAACTGTTT TTCAGTCTCC AGCTGATTAT AAGCAAACCT TTACCTCTTG CATCATAGAA 
CATCTAAATA TATTGCTGTT TGAGTTGGCA CAAAGGTTAT ACAAAGCTCT TTCAAAAGTT 
GACATATCGT TTTACACATC ATTGAAAGGA GAGAAAATGA AAACTGTAGA AAATAATTTG 
CCATCCTGTC ATCATGATCA ACCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT 
AAGGGTCGTC TCTTTTATAC ATGTGATAGA CCCAAAGCGG ATCAATGTAA ATTTTTCAAG 
TGGCTTGAGG AAGTAACTCC TGGATGGTTA ACACAGGAAG AGTCTCAACC CAGCATGGTT 
TTAAATGATA TTAAGAGTAT TGCCTTGTAT TTAAGAAGTC AAAAGATACC ACTCTATGAG 
GAATGCCATC TTTTGGTGAG GAAAGGGTTT GATTTTCAGA GAAAACAGTA TGGCAAACTA 
AAAAAGTTTA CTGCTGTAAA TCCTGAATTT TATAGTGAAC AGAAAAGCAA ACTTTATCTT 
AAATTGAGTC GGAAAGAAGG TTCTTCGGTT TATAGCAAAG ATGATCTTTG GGTGATTTCA 
AAAACCCTAG ACTTTGAACT GGATACTTTT ATTGCATGCA GTGCTTTCTT TGGACCATCA 
TCTCTCAATG AGGTGGAGCT ACTGCCCTTG AAAGGTTATT TCCCTTCTAA TTGGCCTACT 
AACACAGTGG TCCATGCATT ATTAGTTTGT AATGCCAGCA CAGAGCTGAC GACTTTGAAA 
AATATTCAGG ACTACTTTAA TCCAGCTGCT TTACCTCTAA TGCCATATTT GTTAACAATG 
TCTCCGTCAA CTACAGTTAG CAACAAGAGA GAAAATAAGA GAAAATTTAT CCCACCAGCC 
TTTGCAAAAA TCAATACAAA ATTTGAACTA CTTAGCCTGG GAGAAACATT GCAGTTAGCT 
AGTGAGTTAA TTCAGACACA CAAGTTAAAC AAGGACCAAG CTACAGCTCT AATTCAGATA 
GCTCAAATGA TGGCATCCCA TGAAAGTGTT GAAGAAGGGA AGGAACTGCA CACCCATACC 
CTCCCTATCA CAGTCATACA TGGTGTTTTT GGAGCAGGGA AGAGTTATTT GCTGGCGGTG 
GTGATTTTGT TCTTTGTACA ACTATTTGAA AAGACTGAAG CTCTTACAGT TGCAAATGCA 
AGGCCATGGA AACTTCTGAT TTCTTCTTCC ACTAATGTAG CTGTTGACAG AGTACTTCTT 
GGCCTTCTCA ACCTTGGATT TGAAAAGTTT ATCAGAGTTG GGAGTGTAAG AAAGATTGCC 
AAACCAATTT TACCTTATAG CTTGCATGCT GGTTCAGAAA ATGAAAATGA ACAGTTAAAA 
GAACTACATG CACTAATGAA AGAAGATCTA ACCCCTGTGG AAAGAGTCTA TGTAAGAAAA 
AGTATTGAGC AGCATAAACT GGGGACCAAC AAAACCCTGC TGAAGCAGGT TCGAGTAGTT 
GGAGTTACCT GTGCAGCCTG CCCATTCCCA TGCATGAATG ACCTTCAGTT CCCTGTGGTT 
GTGCTGGATG AGTGTAGTCA AATAACAGAA CCAGCCTCTC TCCTTCCCAT TGCAAGGTTT 
AAATGTGAAA AGCTGATTCT TGTTGGAGAC CCCAAACAGC TGCCTCCTAC TATTCAAGGT 
TCTGATGCAG TTCATGAAAA TGGATTGGAA CAAACTCTTT TTGATCGACT TTGTATAATG 
GGTCATAAGC CAATTCTGTT GAGAACCCAG TATCGTTGTC ATCCTGCAAT CAGTGCTATT 
GCCAATGATC TATTTTATGA AGGAAACCTC TTGAATGGCA TTTCAGAGAT AGAGAGGAGC 
CCTTTATTGG AGTGGCTACC CACCCTGTGT TTTTATAATG TTAAAGGACT GGAACAGATT 
GAAAAAGATA ACAGCTTTCA TAATGTGGCA GAAGCTGCTT TTACTCTCAA GTTGATTCAA 
TCATTGATAG CAAGTGGAAT CGCCAGCCCT ATGATTGGAG TGATAACATT ATACAAATCC 
CAGATGTACA AGCTCTGTCA TTTACTCAAT GCTGTGGACT TTGACCATCC TAATATTAAA 
AGTGTGCAGG TATCCACAGT AGATGCTTTC CAGGGCGCTG AAAAGGAGAT CATTATTCTT 
TCCTGTGTAA GGACAAGACA AGTAGGATTC ATTGATTCAG AAAAACGAAT GAATGTTGCA 
CTGACCAGAG GAAGGAGGCA TTTGTTGATT GTGGGAAATT TAGTCTGTTT GAGAAAAAAC 
CGACTATGGG GGCGTGTGAT CCAACATTGT GAAGGAAGTG AAAATGGATT ACAACATGCA 
AGCCAGTGTG AACCCCACCT AAACAAGCTG CTTAAAGATT ATTTTGAAAA ACAAGCAGAA 
GAAAAACGGA GTAAAAAGAC TGAAAAAGAA AAATCTAAAG ATAAATCTCA TTCACAAAAA 
GACATAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021585338.1
CDS	215..5866
Translation	MENQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGVCLENLFLKCLEVKPGDDLESERYLITVEEVKVSGSKTIKQNIIKEAPQSDSRKFISSGRSLGCQPAGLKRKFPGFKAPRQVPTKVIVTENGESAASLESKEPGHTFSSPFNSIPSLFSTVGKKDISTIPRDLENIVTYENRERNSMSLSSVVSTSSFMINPEMLGEKNYFCSPVSTGSQHSDSSLNNEPLRRDNLASHYPGVSQNIRSKAQILALLKSKSTNMHKDLNSEMTRHVPQVQPQGNLNIPVNPKCLIQPEACAEMKSTENLYDQHQSENTMRNKSRWAMYLSSQSSPVHPSTIGESDVKREPKACEDDMSLNLKDLLVQKRMRNFETCDEKGNEVQLVDDTYQSWNQEEKVEILSFCESSSLLGSCSSADNDGLLSESYIQGNNKIPVNQSDKICLEGSVCIGESAQVVNTGTTQQEHNQPVPSLLESEHLQIESSLSNNSQISDGIADVLSKSSSDSESLNNVHESISNVTGPFLEINFNLSNFETSDTEEESQENKISQDSERWVKEILSHDGNLCVQKKSEDTSCQEIGSVCLPLLTSIDSKPTEIFPIKETVSQFCDKTCVGFDMGPYKDRNTGKEIEEEYSDTLSNFDSSLEWNDDVYVYNKEDANKSIKKIGINYDFASLSDKSKGINMNLHFPQVLDIFTNQSPKNGALFSENVQPQTFILRSDIDKNEEQILLPASSSDESIQLLNSNQDHCERVALDRSDIQDSNSLFYPLGKKHSILKDYEVYIPESEDLGGIRKLPQDHVEVAIARKSKQSWNNSRNSSELSGVINNISLLKSLSEHSTALESLETLKKKNTGFQQQRIQQTYDPDCSPEARKPFAIGVSKTIPKSPDRKQDSQQILKEDEVEPSEPFPSLQPVEFQGHQLKGSATSGVMVRGKSSQLVYSQFPDSMEYQNFMPDTCFLTPELPSSCMHTDFLQVKSPEQKFSTLSPVSTFSLNSRNEDVLKFSEESLKARTLPGMKKMSSLKNKNLQQLPLEINRSELCFPSGQKVKCAYLPKRQIHIPTVFQSPADYKQTFTSCIIEHLNILLFELAQRLYKALSKVDISFYTSLKGEKMKTVENNLPSCHHDQPAKLVMVKKEGPNKGRLFYTCDRPKADQCKFFKWLEEVTPGWLTQEESQPSMVLNDIKSIALYLRSQKIPLYEECHLLVRKGFDFQRKQYGKLKKFTAVNPEFYSEQKSKLYLKLSRKEGSSVYSKDDLWVISKTLDFELDTFIACSAFFGPSSLNEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIQDYFNPAALPLMPYLLTMSPSTTVSNKRENKRKFIPPAFAKINTKFELLSLGETLQLASELIQTHKLNKDQATALIQIAQMMASHESVEEGKELHTHTLPITVIHGVFGAGKSYLLAVVILFFVQLFEKTEALTVANARPWKLLISSSTNVAVDRVLLGLLNLGFEKFIRVGSVRKIAKPILPYSLHAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLQFPVVVLDECSQITEPASLLPIARFKCEKLILVGDPKQLPPTIQGSDAVHENGLEQTLFDRLCIMGHKPILLRTQYRCHPAISAIANDLFYEGNLLNGISEIERSPLLEWLPTLCFYNVKGLEQIEKDNSFHNVAEAAFTLKLIQSLIASGIASPMIGVITLYKSQMYKLCHLLNAVDFDHPNIKSVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLVCLRKNRLWGRVIQHCEGSENGLQHASQCEPHLNKLLKDYFEKQAEEKRSKKTEKEKSKDKSHSQKDII

Target ORF information:

RefSeq Version	XM_021729663.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus zinc finger GRF-type containing 1 (Zgrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021729663.1

ORF Insert Sequence:

ATGGAAAACC AAGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGTAT GTTTGGAAAA TCTATTTCTT AAGTGTCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGCG AACGATACTT AATCACAGTT GAGGAGGTTA AAGTTTCTGG AAGCAAAACT 
ATTAAACAGA ATATTATTAA AGAAGCACCA CAGTCAGATT CAAGGAAGTT CATATCCTCT 
GGCCGGTCTC TTGGATGTCA GCCTGCTGGT TTAAAAAGAA AGTTTCCTGG TTTTAAAGCA 
CCACGTCAGG TCCCAACAAA AGTGATTGTT ACAGAAAATG GTGAATCAGC TGCATCACTT 
GAGTCTAAGG AACCTGGCCA TACTTTCTCT TCTCCATTCA ACAGCATACC TTCTTTGTTT 
TCTACTGTTG GCAAGAAAGA TATAAGTACT ATACCAAGAG ACCTTGAAAA TATTGTTACT 
TATGAGAACA GAGAGAGAAA TAGCATGTCT TTGTCTTCAG TTGTCTCTAC TTCATCCTTC 
ATGATTAACC CAGAAATGCT AGGTGAAAAA AATTATTTTT GTTCTCCTGT CAGTACTGGA 
AGTCAGCATT CAGACTCTTC ACTGAACAAT GAACCCCTAA GAAGAGACAA TTTGGCATCT 
CACTATCCAG GAGTTTCACA AAATATTAGA AGTAAAGCCC AGATATTAGC TCTTTTGAAA 
TCTAAGTCAA CCAATATGCA TAAGGACCTA AATTCTGAGA TGACAAGACA TGTACCTCAG 
GTACAGCCAC AAGGAAATTT AAACATTCCT GTGAACCCAA AGTGCTTAAT TCAACCGGAA 
GCATGTGCTG AGATGAAGAG CACAGAAAAT TTATATGACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC CGTACATCCC 
TCTACTATAG GTGAGAGCGA TGTAAAAAGG GAACCTAAAG CTTGTGAGGA TGATATGAGT 
TTGAATTTGA AAGACCTTTT GGTACAAAAA AGGATGCGGA ACTTTGAAAC GTGTGATGAA 
AAGGGAAATG AAGTCCAGCT AGTAGATGAT ACCTATCAAT CCTGGAATCA GGAAGAAAAA 
GTAGAAATAC TTTCTTTCTG TGAAAGCAGC AGCTTACTGG GTAGTTGTAG CAGTGCAGAT 
AATGATGGGT TATTATCAGA ATCTTACATT CAAGGAAATA ATAAAATACC TGTTAATCAA 
AGTGACAAGA TATGCTTGGA AGGATCAGTT TGCATTGGAG AAAGTGCTCA GGTTGTCAAT 
ACAGGTACAA CACAACAGGA GCATAATCAA CCAGTGCCAT CTCTGCTGGA ATCAGAACAT 
CTACAGATCG AATCTTCTCT AAGTAACAAT TCTCAGATCT CTGATGGCAT TGCAGATGTG 
CTTTCTAAAA GTAGTAGTGA TAGTGAAAGT CTAAATAATG TTCATGAATC TATAAGTAAT 
GTAACAGGAC CATTTTTGGA GATAAATTTT AATCTGAGCA ATTTTGAGAC CAGTGACACA 
GAAGAGGAAT CACAGGAAAA CAAAATTTCC CAGGATTCAG AGAGATGGGT AAAGGAAATT 
TTGTCTCACG ATGGCAATTT ATGTGTTCAA AAGAAAAGTG AGGATACAAG CTGTCAAGAA 
ATTGGCAGTG TATGTTTGCC ACTCTTAACA TCTATTGATA GCAAACCTAC AGAGATATTT 
CCTATTAAAG AGACTGTATC ACAGTTTTGT GATAAAACTT GTGTGGGTTT TGACATGGGG 
CCTTATAAAG ATAGAAACAC TGGAAAAGAA ATAGAGGAAG AATATAGTGA CACATTAAGC 
AATTTTGACT CATCTTTAGA ATGGAATGAT GATGTATATG TATATAATAA AGAAGATGCT 
AATAAATCTA TCAAAAAAAT TGGAATTAAC TATGATTTTG CTTCACTTTC AGATAAGTCA 
AAAGGTATAA ACATGAATTT ACATTTTCCT CAAGTTTTAG ACATATTCAC TAATCAGAGT 
CCTAAAAATG GTGCCCTATT CTCAGAAAAT GTTCAACCTC AGACTTTTAT TTTGAGAAGT 
GACATAGACA AAAATGAGGA GCAGATTTTA CTCCCAGCTT CTAGCAGTGA TGAAAGTATC 
CAACTATTAA ATTCCAATCA GGATCACTGT GAACGTGTTG CACTTGATAG ATCAGATATC 
CAAGATTCTA ATTCTTTGTT TTATCCTCTG GGAAAAAAAC ATTCTATTTT AAAAGACTAT 
GAAGTATATA TTCCTGAATC TGAAGACTTG GGAGGAATTA GAAAGTTACC CCAAGACCAT 
GTTGAAGTTG CAATTGCCAG AAAAAGCAAA CAATCCTGGA ATAATTCTAG AAATTCTTCA 
GAACTTTCTG GGGTAATAAA TAACATTTCT CTTTTAAAGT CACTGTCTGA ACACAGTACA 
GCTTTAGAAA GCTTGGAAAC ACTGAAAAAG AAAAATACTG GCTTTCAACA ACAAAGAATT 
CAGCAGACAT ATGATCCAGA TTGCAGTCCT GAAGCTAGGA AACCATTTGC TATAGGAGTT 
TCAAAAACAA TACCAAAGTC TCCTGATCGG AAGCAGGATT CTCAACAGAT ACTAAAAGAA 
GATGAAGTTG AACCAAGTGA ACCATTTCCG TCTCTGCAGC CTGTTGAGTT TCAAGGACAC 
CAACTGAAAG GATCAGCTAC TAGTGGTGTA ATGGTCAGAG GAAAGAGCTC ACAGCTAGTA 
TACAGCCAGT TTCCAGATAG CATGGAATAT CAAAACTTCA TGCCAGATAC TTGTTTTTTG 
ACACCAGAGT TGCCTAGCTC TTGTATGCAT ACTGACTTCT TGCAGGTGAA GTCACCAGAA 
CAAAAGTTCT CTACATTGAG TCCTGTTTCT ACTTTTTCTT TGAACTCAAG AAATGAAGAT 
GTTCTAAAAT TCTCTGAAGA GTCCCTGAAA GCAAGAACTT TACCTGGAAT GAAAAAAATG 
AGTTCTCTGA AGAATAAAAA CCTTCAACAG CTCCCATTAG AGATAAACCG CTCGGAGCTA 
TGCTTCCCAA GTGGACAAAA AGTAAAATGT GCTTATCTTC CCAAAAGGCA GATTCACATA 
CCAACTGTTT TTCAGTCTCC AGCTGATTAT AAGCAAACCT TTACCTCTTG CATCATAGAA 
CATCTAAATA TATTGCTGTT TGAGTTGGCA CAAAGGTTAT ACAAAGCTCT TTCAAAAGTT 
GACATATCGT TTTACACATC ATTGAAAGGA GAGAAAATGA AAACTGTAGA AAATAATTTG 
CCATCCTGTC ATCATGATCA ACCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT 
AAGGGTCGTC TCTTTTATAC ATGTGATAGA CCCAAAGCGG ATCAATGTAA ATTTTTCAAG 
TGGCTTGAGG AAGTAACTCC TGGATGGTTA ACACAGGAAG AGTCTCAACC CAGCATGGTT 
TTAAATGATA TTAAGAGTAT TGCCTTGTAT TTAAGAAGTC AAAAGATACC ACTCTATGAG 
GAATGCCATC TTTTGGTGAG GAAAGGGTTT GATTTTCAGA GAAAACAGTA TGGCAAACTA 
AAAAAGTTTA CTGCTGTAAA TCCTGAATTT TATAGTGAAC AGAAAAGCAA ACTTTATCTT 
AAATTGAGTC GGAAAGAAGG TTCTTCGGTT TATAGCAAAG ATGATCTTTG GGTGATTTCA 
AAAACCCTAG ACTTTGAACT GGATACTTTT ATTGCATGCA GTGCTTTCTT TGGACCATCA 
TCTCTCAATG AGGTGGAGCT ACTGCCCTTG AAAGGTTATT TCCCTTCTAA TTGGCCTACT 
AACACAGTGG TCCATGCATT ATTAGTTTGT AATGCCAGCA CAGAGCTGAC GACTTTGAAA 
AATATTCAGG ACTACTTTAA TCCAGCTGCT TTACCTCTAA TGCCATATTT GTTAACAATG 
TCTCCGTCAA CTACAGTTAG CAACAAGAGA GAAAATAAGA GAAAATTTAT CCCACCAGCC 
TTTGCAAAAA TCAATACAAA ATTTGAACTA CTTAGCCTGG GAGAAACATT GCAGTTAGCT 
AGTGAGTTAA TTCAGACACA CAAGTTAAAC AAGGACCAAG CTACAGCTCT AATTCAGATA 
GCTCAAATGA TGGCATCCCA TGAAAGTGTT GAAGAAGGGA AGGAACTGCA CACCCATACC 
CTCCCTATCA CAGTCATACA TGGTGTTTTT GGAGCAGGGA AGAGTTATTT GCTGGCGGTG 
GTGATTTTGT TCTTTGTACA ACTATTTGAA AAGACTGAAG CTCTTACAGT TGCAAATGCA 
AGGCCATGGA AACTTCTGAT TTCTTCTTCC ACTAATGTAG CTGTTGACAG AGTACTTCTT 
GGCCTTCTCA ACCTTGGATT TGAAAAGTTT ATCAGAGTTG GGAGTGTAAG AAAGATTGCC 
AAACCAATTT TACCTTATAG CTTGCATGCT GGTTCAGAAA ATGAAAATGA ACAGTTAAAA 
GAACTACATG CACTAATGAA AGAAGATCTA ACCCCTGTGG AAAGAGTCTA TGTAAGAAAA 
AGTATTGAGC AGCATAAACT GGGGACCAAC AAAACCCTGC TGAAGCAGGT TCGAGTAGTT 
GGAGTTACCT GTGCAGCCTG CCCATTCCCA TGCATGAATG ACCTTCAGTT CCCTGTGGTT 
GTGCTGGATG AGTGTAGTCA AATAACAGAA CCAGCCTCTC TCCTTCCCAT TGCAAGGTTT 
AAATGTGAAA AGCTGATTCT TGTTGGAGAC CCCAAACAGC TGCCTCCTAC TATTCAAGGT 
TCTGATGCAG TTCATGAAAA TGGATTGGAA CAAACTCTTT TTGATCGACT TTGTATAATG 
GGTCATAAGC CAATTCTGTT GAGAACCCAG TATCGTTGTC ATCCTGCAAT CAGTGCTATT 
GCCAATGATC TATTTTATGA AGGAAACCTC TTGAATGGCA TTTCAGAGAT AGAGAGGAGC 
CCTTTATTGG AGTGGCTACC CACCCTGTGT TTTTATAATG TTAAAGGACT GGAACAGATT 
GAAAAAGATA ACAGCTTTCA TAATGTGGCA GAAGCTGCTT TTACTCTCAA GTTGATTCAA 
TCATTGATAG CAAGTGGAAT CGCCAGCCCT ATGATTGGAG TGATAACATT ATACAAATCC 
CAGATGTACA AGCTCTGTCA TTTACTCAAT GCTGTGGACT TTGACCATCC TAATATTAAA 
AGTGTGCAGG TATCCACAGT AGATGCTTTC CAGGGCGCTG AAAAGGAGAT CATTATTCTT 
TCCTGTGTAA GGACAAGACA AGTAGGATTC ATTGATTCAG AAAAACGAAT GAATGTTGCA 
CTGACCAGAG GAAGGAGGCA TTTGTTGATT GTGGGAAATT TAGTCTGTTT GAGAAAAAAC 
CGACTATGGG GGCGTGTGAT CCAACATTGT GAAGGAAGTG AAAATGGATT ACAACATGCA 
AGCCAGTGTG AACCCCACCT AAACAAGCTG CTTAAAGATT ATTTTGAAAA ACAAGCAGAA 
GAAAAACGGA GTAAAAAGAC TGAAAAAGAA AAATCTAAAG ATAAATCTCA TTCACAAAAA 
GACATAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_013360672.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013216126.1 Ictidomys tridecemlineatus zinc finger, GRF-type containing 1 (Zgrf1), mRNA.

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CloneID	OIc61116
Clone ID Related Accession (Same CDS sequence)	XM_013360672.1
Accession Version	XM_013360672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	LOW QUALITY PROTEIN: protein ZGRF1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAAACC AAGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGTAT GTTTGGAAAA TCTATTTCTT AAGTGTCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGCG AACGATACTT AATCACAGTT GAGGAGGTTA AAGTTTCTGG AAGCAAAACT 
ATTAAACAGA ATATTATTAA AGAAGCACCA CAGTCAGATT CAAGGAAGTT CATATCCTCT 
GGCCGGTCTC TTGGATGTCA GCCTGCTGGT TTAAAAAGAA AGTTTCCTGG TTTTAAAGCA 
CCACGTCAGG TCCCAACAAA AGTGATTGTT ACAGAAAATG GTGAATCAGC TGCATCACTT 
GAGTCTAAGG AACCTGGCCA TACTTTCTCT TCTCCATTCA ACAGCATACC TTCTTTGTTT 
TCTACTGTTG GCAAGAAAGA TATAAGTACT ATACCAAGAG ACCTTGAAAA TATTGTTACT 
TATGAGAACA GAGAGAGAAA TAGCATGTCT TTGTCTTCAG TTGTCTCTAC TTCATCCTTC 
ATGATTAACC CAGAAATGCT AGGTGAAAAA AATTATTTTT GTTCTCCTGT CAGTACTGGA 
AGTCAGCATT CAGACTCTTC ACTGAACAAT GAACCCCTAA GAAGAGACAA TTTGGCATCT 
CACTATCCAG GAGTTTCACA AAATATTAGA AGTAAAGCCC AGATATTAGC TCTTTTGAAA 
TCTAAGTCAA CCAATATGCA TAAGGACCTA AATTCTGAGA TGACAAGACA TGTACCTCAG 
GTACAGCCAC AAGGAAATTT AAACATTCCT GTGAACCCAA AGTGCTTAAT TCAACCGGAA 
GCATGTGCTG AGATGAAGAG CACAGAAAAT TTATATGACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC CGTACATCCC 
TCTACTATAG GTGAGAGCGA TGTAAAAAGG GAACCTAAAG CTTGTGAGGA TGATATGAGT 
TTGAATTTGA AAGACCTTTT GGTACAAAAA AGGATGCGGA ACTTTGAAAC GTGTGATGAA 
AAGGGAAATG AAGTCCAGCT AGTAGATGAT ACCTATCAAT CCTGGAATCA GGAAGAAAAA 
GTAGAAATAC TTTCTTTCTG TGAAAGCAGC AGCTTACTGG GTAGTTGTAG CAGTGCAGAT 
AATGATGGGT TATTATCAGA ATCTTACATT CAAGGAAATA ATAAAATACC TGTTAATCAA 
AGTGACAAGA TATGCTTGGA AGGATCAGTT TGCATTGGAG AAAGTGCTCA GGTTGTCAAT 
ACAGGTACAA CACAACAGGA GCATAATCAA CCAGTGCCAT CTCTGCTGGA ATCAGAACAT 
CTACAGATCG AATCTTCTCT AAGTAACAAT TCTCAGATCT CTGATGGCAT TGCAGATGTG 
CTTTCTAAAA GTAGTAGTGA TAGTGAAAGT CTAAATAATG TTCATGAATC TATAAGTAAT 
GTAACAGGAC CATTTTTGGA GATAAATTTT AATCTGAGCA ATTTTGAGAC CAGTGACACA 
GAAGAGGAAT CACAGGAAAA CAAAATTTCC CAGGATTCAG AGAGATGGGT AAAGGAAATT 
TTGTCTCACG ATGGCAATTT ATGTGTTCAA AAGAAAAGTG AGGATACAAG CTGTCAAGAA 
ATTGGCAGTG TATGTTTGCC ACTCTTAACA TCTATTGATA GCAAACCTAC AGAGATATTT 
CCTATTAAAG AGACTGTATC ACAGTTTTGT GATAAAACTT GTGTGGGTTT TGACATGGGG 
CCTTATAAAG ATAGAAACAC TGGAAAAGAA ATAGAGGAAG AATATAGTGA CACATTAAGC 
AATTTTGACT CATCTTTAGA ATGGAATGAT GATGTATATG TATATAATAA AGAAGATGCT 
AATAAATCTA TCAAAAAAAT TGGAATTAAC TATGATTTTG CTTCACTTTC AGATAAGTCA 
AAAGGTATAA ACATGAATTT ACATTTTCCT CAAGTTTTAG ACATATTCAC TAATCAGAGT 
CCTAAAAATG GTGCCCTATT CTCAGAAAAT GTTCAACCTC AGACTTTTAT TTTGAGAAGT 
GACATAGACA AAAATGAGGA GCAGATTTTA CTCCCAGCTT CTAGCAGTGA TGAAAGTATC 
CAACTATTAA ATTCCAATCA GGATCACTGT GAACGTGTTG CACTTGATAG ATCAGATATC 
CAAGATTCTA ATTCTTTGTT TTATCCTCTG GGAAAAAAAC ATTCTATTTT AAAAGACTAT 
GAAGTATATA TTCCTGAATC TGAAGACTTG GGAGGAATTA GAAAGTTACC CCAAGACCAT 
GTTGAAGTTG CAATTGCCAG AAAAAGCAAA CAATCCTGGA ATAATTCTAG AAATTCTTCA 
GAACTTTCTG GGGTAATAAA TAACATTTCT CTTTTAAAGT CACTGTCTGA ACACAGTACA 
GCTTTAGAAA GCTTGGAAAC ACTGAAAAAG AAAAATACTG GCTTTCAACA ACAAAGAATT 
CAGCAGACAT ATGATCCAGA TTGCAGTCCT GAAGCTAGGA AACCATTTGC TATAGGAGTT 
TCAAAAACAA TACCAAAGTC TCCTGATCGG AAGCAGGATT CTCAACAGAT ACTAAAAGAA 
GATGAAGTTG AACCAAGTGA ACCATTTCCG TCTCTGCAGT TCTTTTCCTT GGGAAGTGAA 
GAAGAGATTA CATTTCTAGC TGTTATTCCT AAACAAATAG AAAGAAAAAC CTGTGACCCA 
AGCCTTTGCT CTGAGAGCAG TAAGACTGGA TCTTCTGCTG GATGCAGGCC TGTTGAGTTT 
CAAGGACACC AACTGAAAGG ATCAGCTACT AGTGGTGTAA TGGTCAGAGG AAAGAGCTCA 
CAGCTAGTAT ACAGCCAGTT TCCAGATAGC ATGGAATATC AAAACTTCAT GCCAGATACT 
TGTTTTTTGA CACCAGAGTT GCCTAGCTCT TGTATGCATA CTGACTTCTT GCAGGTGAAG 
TCACCAGAAC AAAAGTTCTC TACATTGAGT CCTGTTTCTA CTTTTTCTTT GAACTCAAGA 
AATGAAGATG TTCTAAAATT CTCTGAAGAG TCCCTGAAAG CAAGAACTTT ACCTGGTGAC 
CTTGACTTTC TCTCCTTGGA AGGAATGAAA AAAATGAGTT CTCTGAAGAA TAAAAACCTT 
CAACAGCTCC CATTAGTAAG TAGAGCTGGA GTTCCTTCTC CTGAACTGCC TAACTTTGAT 
TCTTATTCAT TTGTGTTCAA CTGTNNGACA CATCAGTCTT CTGATTTCCC CACATTCAAC 
TTGCAGGAAG AGTCAGCAGT TTTTTCCTTT AGCTTTGGGT CTGAGGACCA AAGTGAAACT 
TTCTCTGAAG AAGCTATGAA AATGACTCCA GGGGATGTTT CACTGCTCAA TACAAAGTCT 
CAAAGCAAGT GGCTAAAATA TGAAAATACA TCCCAATGTA ACTTGACTAC TCCAAACAGA 
GTTGATCTAG GAACGGATGG CTTCTTCACT GAGGCTATTT CTGGGATGCA TTTTAGTGAC 
TGCTGTGAAG GACAGAGTGA GGCTCTGAAT GAAAGTTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCAG CAAGGTTTTA GCAGTCAAGA TTTGGTTTCC 
AGAAAGAATG CACTTTCTTT GAGTTTAAAG CAGACTTCCA AATCTGAGGA GGCTCAAAAT 
GTGTTAGGAA AGTCCACCTG CTACAACTAT AGTGCACAGG ATTTACAGGA GATAAACCGC 
TCGGAGCTAT GCTTCCCAAG TGGACAAAAA GTAAAATGTG CTTATCTTCC CAAAAGGCAG 
ATTCACATAC CAACTGTTTT TCAGTCTCCA GCTGATTATA AGCAAACCTT TACCTCTTGC 
ATCATAGAAC ATCTAAATAT ATTGCTGTTT GAGTTGGCAC AAAGGTTATA CAAAGCTCTT 
TCAAAAGTTG ACATATCGTT TTACACATCA TTGAAAGGAG AGAAAATGAA AACTGTAGAA 
AATAATTTGC CATCCTGTCA TCATGATCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATAGAC CCAAAGCGGA TCAATGTAAA 
TTTTTCAAGT GGCTTGAGGA AGTAACTCCT GGATGGTTAA CACAGGAAGA GTCTCAACCC 
AGCATGGTTT TAAATGATAT TAAGAGTATT GCCTTGTATT TAAGAAGTCA AAAGATACCA 
CTCTATGAGG AATGCCATCT TTTGGTGAGG AAAGGGTTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AAAAGTTTAC TGCTGTAAAT CCTGAATTTT ATAGTGAACA GAAAAGCAAA 
CTTTATCTTA AATTGAGTCG GAAAGAAGGT TCTTCGGTTT ATAGCAAAGA TGATCTTTGG 
GTGATTTCAA AAACCCTAGA CTTTGAACTG GATACTTTTA TTGCATGCAG TGCTTTCTTT 
GGACCATCAT CTCTCAATGA GGTGGAGCTA CTGCCCTTGA AAGGTTATTT CCCTTCTAAT 
TGGCCTACTA ACACAGTGGT CCATGCATTA TTAGTTTGTA ATGCCAGCAC AGAGCTGACG 
ACTTTGAAAA ATATTCAGGA CTACTTTAAT CCAGCTGCTT TACCTCTAAT GCCATATTTG 
TTAACAATGT CTCCGTCAAC TACAGTTAGC AACAAGAGAG AAAATAAGAG AAAATTTATC 
CCACCAGCCT TTGCAAAAAT CAATACAAAA TTTGAACTAC TTAGCCTGGG AGAAACATTG 
CAGTTAGCTA GTGAGTTAAT TCAGACACAC AAGTTAAACA AGGACCAAGC TACAGCTCTA 
ATTCAGATAG CTCAAATGAT GGCATCCCAT GAAAGTGTTG AAGAAGGGAA GGAACTGCAC 
ACCCATACCC TCCCTATCAC AGTCATACAT GGTGTTTTTG GAGCAGGGAA GAGTTATTTG 
CTGGCGGTGG TGATTTTGTT CTTTGTACAA CTATTTGAAA AGACTGAAGC TCTTACAGTT 
GCAAATGCAA GGCCATGGAA ACTTCTGATT TCTTCTTCCA CTAATGTAGC TGTTGACAGA 
GTACTTCTTG GCCTTCTCAA CCTTGGATTT GAAAAGTTTA TCAGAGTTGG GAGTGTAAGA 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GTTCAGAAAA TGAAAATGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGATCTAA CCCCTGTGGA AAGAGTCTAT 
GTAAGAAAAA GTATTGAGCA GCATAAACTG GGGACCAACA AAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA CCTTCAGTTC 
CCTGTGGTTG TGCTGGATGA GTGTAGTCAA ATAACAGAAC CAGCCTCTCT CCTTCCCATT 
GCAAGGTTTA AATGTGAAAA GCTGATTCTT GTTGGAGACC CCAAACAGCT GCCTCCTACT 
ATTCAAGGTT CTGATGCAGT TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGTATAATGG GTCATAAGCC AATTCTGTTG AGAACCCAGT ATCGTTGTCA TCCTGCAATC 
AGTGCTATTG CCAATGATCT ATTTTATGAA GGAAACCTCT TGAATGGCAT TTCAGAGATA 
GAGAGGAGCC CTTTATTGGA GTGGCTACCC ACCCTGTGTT TTTATAATGT TAAAGGACTG 
GAACAGATTG AAAAAGATAA CAGCTTTCAT AATGTGGCAG AAGCTGCTTT TACTCTCAAG 
TTGATTCAAT CATTGATAGC AAGTGGAATC GCCAGCCCTA TGATTGGAGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTCTGTCAT TTACTCAATG CTGTGGACTT TGACCATCCT 
AATATTAAAA GTGTGCAGGT ATCCACAGTA GATGCTTTCC AGGGCGCTGA AAAGGAGATC 
ATTATTCTTT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAACGAATG 
AATGTTGCAC TGACCAGAGG AAGGAGGCAT TTGTTGATTG TGGGAAATTT AGTCTGTTTG 
AGAAAAAACC GACTATGGGG GCGTGTGATC CAACATTGTG AAGGAAGTGA AAATGGATTA 
CAACATGCAA GCCAGTGTGA ACCCCACCTA AACAAGCTGC TTAAAGATTA TTTTGAAAAA 
CAAGCAGAAG AAAAACGGAG TAAAAAGACT GAAAAAGAAA AATCTAAAGA TAAATCTCAT 
TCACAAAAAG ACATAATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013216126.1
CDS	1..6381
Translation	MENQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGVCLENLFLKCLEVKPGDDLESERYLITVEEVKVSGSKTIKQNIIKEAPQSDSRKFISSGRSLGCQPAGLKRKFPGFKAPRQVPTKVIVTENGESAASLESKEPGHTFSSPFNSIPSLFSTVGKKDISTIPRDLENIVTYENRERNSMSLSSVVSTSSFMINPEMLGEKNYFCSPVSTGSQHSDSSLNNEPLRRDNLASHYPGVSQNIRSKAQILALLKSKSTNMHKDLNSEMTRHVPQVQPQGNLNIPVNPKCLIQPEACAEMKSTENLYDQHQSENTMRNKSRWAMYLSSQSSPVHPSTIGESDVKREPKACEDDMSLNLKDLLVQKRMRNFETCDEKGNEVQLVDDTYQSWNQEEKVEILSFCESSSLLGSCSSADNDGLLSESYIQGNNKIPVNQSDKICLEGSVCIGESAQVVNTGTTQQEHNQPVPSLLESEHLQIESSLSNNSQISDGIADVLSKSSSDSESLNNVHESISNVTGPFLEINFNLSNFETSDTEEESQENKISQDSERWVKEILSHDGNLCVQKKSEDTSCQEIGSVCLPLLTSIDSKPTEIFPIKETVSQFCDKTCVGFDMGPYKDRNTGKEIEEEYSDTLSNFDSSLEWNDDVYVYNKEDANKSIKKIGINYDFASLSDKSKGINMNLHFPQVLDIFTNQSPKNGALFSENVQPQTFILRSDIDKNEEQILLPASSSDESIQLLNSNQDHCERVALDRSDIQDSNSLFYPLGKKHSILKDYEVYIPESEDLGGIRKLPQDHVEVAIARKSKQSWNNSRNSSELSGVINNISLLKSLSEHSTALESLETLKKKNTGFQQQRIQQTYDPDCSPEARKPFAIGVSKTIPKSPDRKQDSQQILKEDEVEPSEPFPSLQFFSLGSEEEITFLAVIPKQIERKTCDPSLCSESSKTGSSAGCRPVEFQGHQLKGSATSGVMVRGKSSQLVYSQFPDSMEYQNFMPDTCFLTPELPSSCMHTDFLQVKSPEQKFSTLSPVSTFSLNSRNEDVLKFSEESLKARTLPGDLDFLSLEGMKKMSSLKNKNLQQLPLVSRAGVPSPELPNFDSYSFVFNCnullTHQSSDFPTFNLQEESAVFSFSFGSEDQSETFSEEAMKMTPGDVSLLNTKSQSKWLKYENTSQCNLTTPNRVDLGTDGFFTEAISGMHFSDCCEGQSEALNESSLDSVHLQMIKGMLYQQQQGFSSQDLVSRKNALSLSLKQTSKSEEAQNVLGKSTCYNYSAQDLQEINRSELCFPSGQKVKCAYLPKRQIHIPTVFQSPADYKQTFTSCIIEHLNILLFELAQRLYKALSKVDISFYTSLKGEKMKTVENNLPSCHHDQPAKLVMVKKEGPNKGRLFYTCDRPKADQCKFFKWLEEVTPGWLTQEESQPSMVLNDIKSIALYLRSQKIPLYEECHLLVRKGFDFQRKQYGKLKKFTAVNPEFYSEQKSKLYLKLSRKEGSSVYSKDDLWVISKTLDFELDTFIACSAFFGPSSLNEVELLPLKGYFPSNWPTNTVVHALLVCNASTELTTLKNIQDYFNPAALPLMPYLLTMSPSTTVSNKRENKRKFIPPAFAKINTKFELLSLGETLQLASELIQTHKLNKDQATALIQIAQMMASHESVEEGKELHTHTLPITVIHGVFGAGKSYLLAVVILFFVQLFEKTEALTVANARPWKLLISSSTNVAVDRVLLGLLNLGFEKFIRVGSVRKIAKPILPYSLHAGSENENEQLKELHALMKEDLTPVERVYVRKSIEQHKLGTNKTLLKQVRVVGVTCAACPFPCMNDLQFPVVVLDECSQITEPASLLPIARFKCEKLILVGDPKQLPPTIQGSDAVHENGLEQTLFDRLCIMGHKPILLRTQYRCHPAISAIANDLFYEGNLLNGISEIERSPLLEWLPTLCFYNVKGLEQIEKDNSFHNVAEAAFTLKLIQSLIASGIASPMIGVITLYKSQMYKLCHLLNAVDFDHPNIKSVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGNLVCLRKNRLWGRVIQHCEGSENGLQHASQCEPHLNKLLKDYFEKQAEEKRSKKTEKEKSKDKSHSQKDII

Target ORF information:

RefSeq Version	XM_013360672.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus zinc finger, GRF-type containing 1 (Zgrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013360672.1

ORF Insert Sequence:

ATGGAAAACC AAGAATTTAT TGTCCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGTAT GTTTGGAAAA TCTATTTCTT AAGTGTCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGCG AACGATACTT AATCACAGTT GAGGAGGTTA AAGTTTCTGG AAGCAAAACT 
ATTAAACAGA ATATTATTAA AGAAGCACCA CAGTCAGATT CAAGGAAGTT CATATCCTCT 
GGCCGGTCTC TTGGATGTCA GCCTGCTGGT TTAAAAAGAA AGTTTCCTGG TTTTAAAGCA 
CCACGTCAGG TCCCAACAAA AGTGATTGTT ACAGAAAATG GTGAATCAGC TGCATCACTT 
GAGTCTAAGG AACCTGGCCA TACTTTCTCT TCTCCATTCA ACAGCATACC TTCTTTGTTT 
TCTACTGTTG GCAAGAAAGA TATAAGTACT ATACCAAGAG ACCTTGAAAA TATTGTTACT 
TATGAGAACA GAGAGAGAAA TAGCATGTCT TTGTCTTCAG TTGTCTCTAC TTCATCCTTC 
ATGATTAACC CAGAAATGCT AGGTGAAAAA AATTATTTTT GTTCTCCTGT CAGTACTGGA 
AGTCAGCATT CAGACTCTTC ACTGAACAAT GAACCCCTAA GAAGAGACAA TTTGGCATCT 
CACTATCCAG GAGTTTCACA AAATATTAGA AGTAAAGCCC AGATATTAGC TCTTTTGAAA 
TCTAAGTCAA CCAATATGCA TAAGGACCTA AATTCTGAGA TGACAAGACA TGTACCTCAG 
GTACAGCCAC AAGGAAATTT AAACATTCCT GTGAACCCAA AGTGCTTAAT TCAACCGGAA 
GCATGTGCTG AGATGAAGAG CACAGAAAAT TTATATGACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC CGTACATCCC 
TCTACTATAG GTGAGAGCGA TGTAAAAAGG GAACCTAAAG CTTGTGAGGA TGATATGAGT 
TTGAATTTGA AAGACCTTTT GGTACAAAAA AGGATGCGGA ACTTTGAAAC GTGTGATGAA 
AAGGGAAATG AAGTCCAGCT AGTAGATGAT ACCTATCAAT CCTGGAATCA GGAAGAAAAA 
GTAGAAATAC TTTCTTTCTG TGAAAGCAGC AGCTTACTGG GTAGTTGTAG CAGTGCAGAT 
AATGATGGGT TATTATCAGA ATCTTACATT CAAGGAAATA ATAAAATACC TGTTAATCAA 
AGTGACAAGA TATGCTTGGA AGGATCAGTT TGCATTGGAG AAAGTGCTCA GGTTGTCAAT 
ACAGGTACAA CACAACAGGA GCATAATCAA CCAGTGCCAT CTCTGCTGGA ATCAGAACAT 
CTACAGATCG AATCTTCTCT AAGTAACAAT TCTCAGATCT CTGATGGCAT TGCAGATGTG 
CTTTCTAAAA GTAGTAGTGA TAGTGAAAGT CTAAATAATG TTCATGAATC TATAAGTAAT 
GTAACAGGAC CATTTTTGGA GATAAATTTT AATCTGAGCA ATTTTGAGAC CAGTGACACA 
GAAGAGGAAT CACAGGAAAA CAAAATTTCC CAGGATTCAG AGAGATGGGT AAAGGAAATT 
TTGTCTCACG ATGGCAATTT ATGTGTTCAA AAGAAAAGTG AGGATACAAG CTGTCAAGAA 
ATTGGCAGTG TATGTTTGCC ACTCTTAACA TCTATTGATA GCAAACCTAC AGAGATATTT 
CCTATTAAAG AGACTGTATC ACAGTTTTGT GATAAAACTT GTGTGGGTTT TGACATGGGG 
CCTTATAAAG ATAGAAACAC TGGAAAAGAA ATAGAGGAAG AATATAGTGA CACATTAAGC 
AATTTTGACT CATCTTTAGA ATGGAATGAT GATGTATATG TATATAATAA AGAAGATGCT 
AATAAATCTA TCAAAAAAAT TGGAATTAAC TATGATTTTG CTTCACTTTC AGATAAGTCA 
AAAGGTATAA ACATGAATTT ACATTTTCCT CAAGTTTTAG ACATATTCAC TAATCAGAGT 
CCTAAAAATG GTGCCCTATT CTCAGAAAAT GTTCAACCTC AGACTTTTAT TTTGAGAAGT 
GACATAGACA AAAATGAGGA GCAGATTTTA CTCCCAGCTT CTAGCAGTGA TGAAAGTATC 
CAACTATTAA ATTCCAATCA GGATCACTGT GAACGTGTTG CACTTGATAG ATCAGATATC 
CAAGATTCTA ATTCTTTGTT TTATCCTCTG GGAAAAAAAC ATTCTATTTT AAAAGACTAT 
GAAGTATATA TTCCTGAATC TGAAGACTTG GGAGGAATTA GAAAGTTACC CCAAGACCAT 
GTTGAAGTTG CAATTGCCAG AAAAAGCAAA CAATCCTGGA ATAATTCTAG AAATTCTTCA 
GAACTTTCTG GGGTAATAAA TAACATTTCT CTTTTAAAGT CACTGTCTGA ACACAGTACA 
GCTTTAGAAA GCTTGGAAAC ACTGAAAAAG AAAAATACTG GCTTTCAACA ACAAAGAATT 
CAGCAGACAT ATGATCCAGA TTGCAGTCCT GAAGCTAGGA AACCATTTGC TATAGGAGTT 
TCAAAAACAA TACCAAAGTC TCCTGATCGG AAGCAGGATT CTCAACAGAT ACTAAAAGAA 
GATGAAGTTG AACCAAGTGA ACCATTTCCG TCTCTGCAGT TCTTTTCCTT GGGAAGTGAA 
GAAGAGATTA CATTTCTAGC TGTTATTCCT AAACAAATAG AAAGAAAAAC CTGTGACCCA 
AGCCTTTGCT CTGAGAGCAG TAAGACTGGA TCTTCTGCTG GATGCAGGCC TGTTGAGTTT 
CAAGGACACC AACTGAAAGG ATCAGCTACT AGTGGTGTAA TGGTCAGAGG AAAGAGCTCA 
CAGCTAGTAT ACAGCCAGTT TCCAGATAGC ATGGAATATC AAAACTTCAT GCCAGATACT 
TGTTTTTTGA CACCAGAGTT GCCTAGCTCT TGTATGCATA CTGACTTCTT GCAGGTGAAG 
TCACCAGAAC AAAAGTTCTC TACATTGAGT CCTGTTTCTA CTTTTTCTTT GAACTCAAGA 
AATGAAGATG TTCTAAAATT CTCTGAAGAG TCCCTGAAAG CAAGAACTTT ACCTGGTGAC 
CTTGACTTTC TCTCCTTGGA AGGAATGAAA AAAATGAGTT CTCTGAAGAA TAAAAACCTT 
CAACAGCTCC CATTAGTAAG TAGAGCTGGA GTTCCTTCTC CTGAACTGCC TAACTTTGAT 
TCTTATTCAT TTGTGTTCAA CTGTNNGACA CATCAGTCTT CTGATTTCCC CACATTCAAC 
TTGCAGGAAG AGTCAGCAGT TTTTTCCTTT AGCTTTGGGT CTGAGGACCA AAGTGAAACT 
TTCTCTGAAG AAGCTATGAA AATGACTCCA GGGGATGTTT CACTGCTCAA TACAAAGTCT 
CAAAGCAAGT GGCTAAAATA TGAAAATACA TCCCAATGTA ACTTGACTAC TCCAAACAGA 
GTTGATCTAG GAACGGATGG CTTCTTCACT GAGGCTATTT CTGGGATGCA TTTTAGTGAC 
TGCTGTGAAG GACAGAGTGA GGCTCTGAAT GAAAGTTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCAG CAAGGTTTTA GCAGTCAAGA TTTGGTTTCC 
AGAAAGAATG CACTTTCTTT GAGTTTAAAG CAGACTTCCA AATCTGAGGA GGCTCAAAAT 
GTGTTAGGAA AGTCCACCTG CTACAACTAT AGTGCACAGG ATTTACAGGA GATAAACCGC 
TCGGAGCTAT GCTTCCCAAG TGGACAAAAA GTAAAATGTG CTTATCTTCC CAAAAGGCAG 
ATTCACATAC CAACTGTTTT TCAGTCTCCA GCTGATTATA AGCAAACCTT TACCTCTTGC 
ATCATAGAAC ATCTAAATAT ATTGCTGTTT GAGTTGGCAC AAAGGTTATA CAAAGCTCTT 
TCAAAAGTTG ACATATCGTT TTACACATCA TTGAAAGGAG AGAAAATGAA AACTGTAGAA 
AATAATTTGC CATCCTGTCA TCATGATCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATAGAC CCAAAGCGGA TCAATGTAAA 
TTTTTCAAGT GGCTTGAGGA AGTAACTCCT GGATGGTTAA CACAGGAAGA GTCTCAACCC 
AGCATGGTTT TAAATGATAT TAAGAGTATT GCCTTGTATT TAAGAAGTCA AAAGATACCA 
CTCTATGAGG AATGCCATCT TTTGGTGAGG AAAGGGTTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AAAAGTTTAC TGCTGTAAAT CCTGAATTTT ATAGTGAACA GAAAAGCAAA 
CTTTATCTTA AATTGAGTCG GAAAGAAGGT TCTTCGGTTT ATAGCAAAGA TGATCTTTGG 
GTGATTTCAA AAACCCTAGA CTTTGAACTG GATACTTTTA TTGCATGCAG TGCTTTCTTT 
GGACCATCAT CTCTCAATGA GGTGGAGCTA CTGCCCTTGA AAGGTTATTT CCCTTCTAAT 
TGGCCTACTA ACACAGTGGT CCATGCATTA TTAGTTTGTA ATGCCAGCAC AGAGCTGACG 
ACTTTGAAAA ATATTCAGGA CTACTTTAAT CCAGCTGCTT TACCTCTAAT GCCATATTTG 
TTAACAATGT CTCCGTCAAC TACAGTTAGC AACAAGAGAG AAAATAAGAG AAAATTTATC 
CCACCAGCCT TTGCAAAAAT CAATACAAAA TTTGAACTAC TTAGCCTGGG AGAAACATTG 
CAGTTAGCTA GTGAGTTAAT TCAGACACAC AAGTTAAACA AGGACCAAGC TACAGCTCTA 
ATTCAGATAG CTCAAATGAT GGCATCCCAT GAAAGTGTTG AAGAAGGGAA GGAACTGCAC 
ACCCATACCC TCCCTATCAC AGTCATACAT GGTGTTTTTG GAGCAGGGAA GAGTTATTTG 
CTGGCGGTGG TGATTTTGTT CTTTGTACAA CTATTTGAAA AGACTGAAGC TCTTACAGTT 
GCAAATGCAA GGCCATGGAA ACTTCTGATT TCTTCTTCCA CTAATGTAGC TGTTGACAGA 
GTACTTCTTG GCCTTCTCAA CCTTGGATTT GAAAAGTTTA TCAGAGTTGG GAGTGTAAGA 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GTTCAGAAAA TGAAAATGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGATCTAA CCCCTGTGGA AAGAGTCTAT 
GTAAGAAAAA GTATTGAGCA GCATAAACTG GGGACCAACA AAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA CCTTCAGTTC 
CCTGTGGTTG TGCTGGATGA GTGTAGTCAA ATAACAGAAC CAGCCTCTCT CCTTCCCATT 
GCAAGGTTTA AATGTGAAAA GCTGATTCTT GTTGGAGACC CCAAACAGCT GCCTCCTACT 
ATTCAAGGTT CTGATGCAGT TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGTATAATGG GTCATAAGCC AATTCTGTTG AGAACCCAGT ATCGTTGTCA TCCTGCAATC 
AGTGCTATTG CCAATGATCT ATTTTATGAA GGAAACCTCT TGAATGGCAT TTCAGAGATA 
GAGAGGAGCC CTTTATTGGA GTGGCTACCC ACCCTGTGTT TTTATAATGT TAAAGGACTG 
GAACAGATTG AAAAAGATAA CAGCTTTCAT AATGTGGCAG AAGCTGCTTT TACTCTCAAG 
TTGATTCAAT CATTGATAGC AAGTGGAATC GCCAGCCCTA TGATTGGAGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTCTGTCAT TTACTCAATG CTGTGGACTT TGACCATCCT 
AATATTAAAA GTGTGCAGGT ATCCACAGTA GATGCTTTCC AGGGCGCTGA AAAGGAGATC 
ATTATTCTTT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAACGAATG 
AATGTTGCAC TGACCAGAGG AAGGAGGCAT TTGTTGATTG TGGGAAATTT AGTCTGTTTG 
AGAAAAAACC GACTATGGGG GCGTGTGATC CAACATTGTG AAGGAAGTGA AAATGGATTA 
CAACATGCAA GCCAGTGTGA ACCCCACCTA AACAAGCTGC TTAAAGATTA TTTTGAAAAA 
CAAGCAGAAG AAAAACGGAG TAAAAAGACT GAAAAAGAAA AATCTAAAGA TAAATCTCAT 
TCACAAAAAG ACATAATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ZGRF1 cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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