TSC2 gene cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

mRNA	Protein	Name
XM_013364012.2	XP_013219466.1	tuberin isoform X4
XM_013364011.2	XP_013219465.1	tuberin isoform X3
XM_013364013.2	XP_013219467.1	tuberin isoform X5
XM_013364016.2	XP_013219470.1	tuberin isoform X9
XM_013364010.1	XP_013219464.1	tuberin isoform X2
XM_013364012.1	XP_013219466.1	tuberin isoform X4
XM_013364013.1	XP_013219467.1	tuberin isoform X5
XM_005337803.2	XP_005337860.1	tuberin isoform X1
XM_005337803.3	XP_005337860.1	tuberin isoform X1
XM_013364010.2	XP_013219464.1	tuberin isoform X2
XM_013364015.1	XP_013219469.1	tuberin isoform X7
XM_021734944.1	XP_021590619.1	tuberin isoform X6
XM_013364011.1	XP_013219465.1	tuberin isoform X3
XM_013364014.1	XP_013219468.1	tuberin isoform X6
XM_013364014.2	XP_013219468.1	tuberin isoform X7
XM_013364015.2	XP_013219469.1	tuberin isoform X8
XM_013364016.1	XP_013219470.1	tuberin isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc68581	XM_005337803.3 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68582	XM_013364010.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68583	XM_013364011.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68584	XM_013364012.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68585	XM_013364013.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68586	XM_013364014.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68587	XM_013364015.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68588	XM_013364016.2 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84028	XM_021734944.1 Latest version!	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OIc68584	XM_013364012.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68585	XM_013364013.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68581	XM_005337803.2	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68582	XM_013364010.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68583	XM_013364011.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68586	XM_013364014.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68587	XM_013364015.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc68588	XM_013364016.1	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

TSC2 ( XM_005337803.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_005337860.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc68581
Clone ID Related Accession (Same CDS sequence)	XM_005337803.2 , XM_005337803.3
Accession Version	XM_005337803.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005337803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGACATGG AGGGCCTTGT GGACACGAGC 
GTAGCCAAGA TTGTGTCTGA CCGCAACTTG CCCTTTGTGG CTCGCCAGAT GGCCCTGCAC 
GCAAACATGG CCTCACAGGT ACACCACAGT CGCTCCAACC CCACCGACAT CTACCCCTCC 
AAGTGGATCG CCCGGCTCCG CCACATCAAG CGGCTCCGCC ACCGGATCCG TGAGGAAGCC 
CACTACTCGA ACCCTAGCCT GCCTCTGCTA CAGATGCAGT CCACAGCCCA CCCCAGAGCC 
CAGGCTCAGG CCTCAGCTGA GGCCACAGCC ACCTGTGAGG CTGGCCAGCG GAAGCGCCTC 
ATCTCCTCAG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005337860.1
CDS	102..5537
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005337803.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005337803.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGACATGG AGGGCCTTGT GGACACGAGC 
GTAGCCAAGA TTGTGTCTGA CCGCAACTTG CCCTTTGTGG CTCGCCAGAT GGCCCTGCAC 
GCAAACATGG CCTCACAGGT ACACCACAGT CGCTCCAACC CCACCGACAT CTACCCCTCC 
AAGTGGATCG CCCGGCTCCG CCACATCAAG CGGCTCCGCC ACCGGATCCG TGAGGAAGCC 
CACTACTCGA ACCCTAGCCT GCCTCTGCTA CAGATGCAGT CCACAGCCCA CCCCAGAGCC 
CAGGCTCAGG CCTCAGCTGA GGCCACAGCC ACCTGTGAGG CTGGCCAGCG GAAGCGCCTC 
ATCTCCTCAG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_005337803.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_005337860.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

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CloneID	OIc68581
Clone ID Related Accession (Same CDS sequence)	XM_005337803.2 , XM_005337803.3
Accession Version	XM_005337803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005337803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGACATGG AGGGCCTTGT GGACACGAGC 
GTAGCCAAGA TTGTGTCTGA CCGCAACTTG CCCTTTGTGG CTCGCCAGAT GGCCCTGCAC 
GCAAACATGG CCTCACAGGT ACACCACAGT CGCTCCAACC CCACCGACAT CTACCCCTCC 
AAGTGGATCG CCCGGCTCCG CCACATCAAG CGGCTCCGCC ACCGGATCCG TGAGGAAGCC 
CACTACTCGA ACCCTAGCCT GCCTCTGCTA CAGATGCAGT CCACAGCCCA CCCCAGAGCC 
CAGGCTCAGG CCTCAGCTGA GGCCACAGCC ACCTGTGAGG CTGGCCAGCG GAAGCGCCTC 
ATCTCCTCAG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005337860.1
CDS	112..5547
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_005337803.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005337803.3

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGACATGG AGGGCCTTGT GGACACGAGC 
GTAGCCAAGA TTGTGTCTGA CCGCAACTTG CCCTTTGTGG CTCGCCAGAT GGCCCTGCAC 
GCAAACATGG CCTCACAGGT ACACCACAGT CGCTCCAACC CCACCGACAT CTACCCCTCC 
AAGTGGATCG CCCGGCTCCG CCACATCAAG CGGCTCCGCC ACCGGATCCG TGAGGAAGCC 
CACTACTCGA ACCCTAGCCT GCCTCTGCTA CAGATGCAGT CCACAGCCCA CCCCAGAGCC 
CAGGCTCAGG CCTCAGCTGA GGCCACAGCC ACCTGTGAGG CTGGCCAGCG GAAGCGCCTC 
ATCTCCTCAG TGGACGACTT CACCGAGTTT GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364010.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219464.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

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CloneID	OIc68582
Clone ID Related Accession (Same CDS sequence)	XM_013364010.1 , XM_013364010.2
Accession Version	XM_013364010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGGATA CAGACATCCC TCACCAGTGC CAGCCTGGGG 
TCAGCAGACG AGAACTCCAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCATCTGGAG 
CTCACAGAAA CGTGCTTGGA TATGATGGCC CGATACGTGT TCTCCAACTT CACGGCAGTA 
CCTAAAAGGT CTCCTGTGGG AGAATTTCTC CTGGCAGGCG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC AGTGACGACC AGTGTGGGAG CTGGGACCCG CTCATTGCTG 
GGCCTGGACT CTGGGGATCT GCAGGCCAGC CCAGAGTCAA GCTCCAACCC TGGCCTGCAC 
GTGAGACAGA CAAAAGAGGC GCCTGCTAAG CTGGAGTCCC AGACTGGGCA GCAGGTGTCT 
CGCGGGGCCC GGGATCGGGT GCGCTCCATG TCGGGGGGAC ATGGCCTTCG AGTTGCTGCC 
CTGGATGCAC CAGCTTCCCA TATCCCAGGA GGCTGCACTT CTGCAGGACC ACAGAATGTA 
CCAGCTACAA AGCCGGAGAA GGCCTCTGTG GGTGCCCAGG TTCCAGCACC AGAGAAGACG 
AATCTGGCAG CCTATGTACC CCTGCTGACC CAGGGCTGGG CCGAGATCTT GGTCCGAAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTTGAGAACC CACTTAGTCC CTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAACGCCC TCATGGCTGC TGAGCGCTTC 
AAGGAGCACC GTGACACGGC TCTGTACAAG TCACTGTCAG TGCCGGCAGC TGGCACGGCC 
AGGCCCCCTC CTCTCCCACG CTCTAATACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCTGT GGTTCTGGAG 
GAGGGGAGTC CAGGCGAGAC TCATGTACCA GGGGAGCCCC CCGAGTTGGA GGATTTGGAG 
GCAATGCTAA GTGCAGACAG GCGTTGCCGG CGCTCTGAGG CTTACAGCAG GTCATCCTCA 
GCCTCCAGCC AGGAGGAGAA GCCCCACGCT GAGGAGCTGG CTGCGGGGGG CATCCCAATT 
GAGGCAGCAG TCTCCTCTGA GGGTGGCCGG CCCTCCGTGG ACCCCTCCTT CCAGCCATCA 
CAGCCCCTGA GCAAGTCCAG CTCCTCGCCT GAGCTGCAAA CACTGCAGGA CATCCTTGGG 
AACCCTGGGG ACAAGGCTGA TGCTGGCCGG CTGAGCCCTG AGGCCAAGGC CCGATCCCAG 
TCTGGGATCC TGGATGGGGG AAGTGTGGCC TGGGCAGCCC CAGGTGAAGA GGGCCGGGGC 
TCCGCCATAC CCGAGGGTCC TTTGCCATCC AGTTGCCCCC GCTCTCCCGG TGGCCTCCGG 
CCCCGAGGCT ACACCATCTC CGATTCAGCC CCATCGCGCA GGGGCAAGAG GGTGGAGAGG 
GACACTTTGA AGAGCAGAGC TGCAGCCTCC ACTGCTGAGA AAGTGCCTGG CATCAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCACTCG CCATTCTTTG GTGATGAGTC CAACAAGCCC 
ATCCTTTTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAAC TCCTTGACCA GATCCCTTCG 
TATGACACAC ACAAGATTGC TGTGCTGTAT GTGGGCGAAG GCCAGAGCAA CAGCGAGCTG 
GCCATCCTCT CCAATGAGCA CGGCTCCTAC AGGTACACAG AGTTCCTGAC AGGGCTTGGT 
AGGCTCATCG AGCTCAAGGA CTGCCAGCCC GACAAGGTGT ACCTGGGTGG CCTGGACGTG 
TGTGGCGAGG ATGGCCAGTT CACCTACTGT TGGCACGATG ACATCATGCA AGCTGTCTTC 
CATATTGCTA CGCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGA 
CACCTGGGCA ATGACTTTGT CTCCATCATC TACAATGACT CTGGGGAGGA CTTCAAGCTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CACGTGATCA TCACACCCCT GGACTACGAG 
TGCAACCTGC TGTCACTGCA GTGTAGGAAA GACATGGAGG GCCTTGTGGA CACGAGCGTA 
GCCAAGATTG TGTCTGACCG CAACTTGCCC TTTGTGGCTC GCCAGATGGC CCTGCACGCA 
AACATGGCCT CACAGGTACA CCACAGTCGC TCCAACCCCA CCGACATCTA CCCCTCCAAG 
TGGATCGCCC GGCTCCGCCA CATCAAGCGG CTCCGCCACC GGATCCGTGA GGAAGCCCAC 
TACTCGAACC CTAGCCTGCC TCTGCTACAG ATGCAGTCCA CAGCCCACCC CAGAGCCCAG 
GCTCAGGCCT CAGCTGAGGC CACAGCCACC TGTGAGGCTG GCCAGCGGAA GCGCCTCATC 
TCCTCAGTGG ACGACTTCAC CGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219464.1
CDS	102..5534
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364010.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364010.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGGATA CAGACATCCC TCACCAGTGC CAGCCTGGGG 
TCAGCAGACG AGAACTCCAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCATCTGGAG 
CTCACAGAAA CGTGCTTGGA TATGATGGCC CGATACGTGT TCTCCAACTT CACGGCAGTA 
CCTAAAAGGT CTCCTGTGGG AGAATTTCTC CTGGCAGGCG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC AGTGACGACC AGTGTGGGAG CTGGGACCCG CTCATTGCTG 
GGCCTGGACT CTGGGGATCT GCAGGCCAGC CCAGAGTCAA GCTCCAACCC TGGCCTGCAC 
GTGAGACAGA CAAAAGAGGC GCCTGCTAAG CTGGAGTCCC AGACTGGGCA GCAGGTGTCT 
CGCGGGGCCC GGGATCGGGT GCGCTCCATG TCGGGGGGAC ATGGCCTTCG AGTTGCTGCC 
CTGGATGCAC CAGCTTCCCA TATCCCAGGA GGCTGCACTT CTGCAGGACC ACAGAATGTA 
CCAGCTACAA AGCCGGAGAA GGCCTCTGTG GGTGCCCAGG TTCCAGCACC AGAGAAGACG 
AATCTGGCAG CCTATGTACC CCTGCTGACC CAGGGCTGGG CCGAGATCTT GGTCCGAAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTTGAGAACC CACTTAGTCC CTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAACGCCC TCATGGCTGC TGAGCGCTTC 
AAGGAGCACC GTGACACGGC TCTGTACAAG TCACTGTCAG TGCCGGCAGC TGGCACGGCC 
AGGCCCCCTC CTCTCCCACG CTCTAATACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCTGT GGTTCTGGAG 
GAGGGGAGTC CAGGCGAGAC TCATGTACCA GGGGAGCCCC CCGAGTTGGA GGATTTGGAG 
GCAATGCTAA GTGCAGACAG GCGTTGCCGG CGCTCTGAGG CTTACAGCAG GTCATCCTCA 
GCCTCCAGCC AGGAGGAGAA GCCCCACGCT GAGGAGCTGG CTGCGGGGGG CATCCCAATT 
GAGGCAGCAG TCTCCTCTGA GGGTGGCCGG CCCTCCGTGG ACCCCTCCTT CCAGCCATCA 
CAGCCCCTGA GCAAGTCCAG CTCCTCGCCT GAGCTGCAAA CACTGCAGGA CATCCTTGGG 
AACCCTGGGG ACAAGGCTGA TGCTGGCCGG CTGAGCCCTG AGGCCAAGGC CCGATCCCAG 
TCTGGGATCC TGGATGGGGG AAGTGTGGCC TGGGCAGCCC CAGGTGAAGA GGGCCGGGGC 
TCCGCCATAC CCGAGGGTCC TTTGCCATCC AGTTGCCCCC GCTCTCCCGG TGGCCTCCGG 
CCCCGAGGCT ACACCATCTC CGATTCAGCC CCATCGCGCA GGGGCAAGAG GGTGGAGAGG 
GACACTTTGA AGAGCAGAGC TGCAGCCTCC ACTGCTGAGA AAGTGCCTGG CATCAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCACTCG CCATTCTTTG GTGATGAGTC CAACAAGCCC 
ATCCTTTTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAAC TCCTTGACCA GATCCCTTCG 
TATGACACAC ACAAGATTGC TGTGCTGTAT GTGGGCGAAG GCCAGAGCAA CAGCGAGCTG 
GCCATCCTCT CCAATGAGCA CGGCTCCTAC AGGTACACAG AGTTCCTGAC AGGGCTTGGT 
AGGCTCATCG AGCTCAAGGA CTGCCAGCCC GACAAGGTGT ACCTGGGTGG CCTGGACGTG 
TGTGGCGAGG ATGGCCAGTT CACCTACTGT TGGCACGATG ACATCATGCA AGCTGTCTTC 
CATATTGCTA CGCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGA 
CACCTGGGCA ATGACTTTGT CTCCATCATC TACAATGACT CTGGGGAGGA CTTCAAGCTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CACGTGATCA TCACACCCCT GGACTACGAG 
TGCAACCTGC TGTCACTGCA GTGTAGGAAA GACATGGAGG GCCTTGTGGA CACGAGCGTA 
GCCAAGATTG TGTCTGACCG CAACTTGCCC TTTGTGGCTC GCCAGATGGC CCTGCACGCA 
AACATGGCCT CACAGGTACA CCACAGTCGC TCCAACCCCA CCGACATCTA CCCCTCCAAG 
TGGATCGCCC GGCTCCGCCA CATCAAGCGG CTCCGCCACC GGATCCGTGA GGAAGCCCAC 
TACTCGAACC CTAGCCTGCC TCTGCTACAG ATGCAGTCCA CAGCCCACCC CAGAGCCCAG 
GCTCAGGCCT CAGCTGAGGC CACAGCCACC TGTGAGGCTG GCCAGCGGAA GCGCCTCATC 
TCCTCAGTGG ACGACTTCAC CGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364010.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219464.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

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CloneID	OIc68582
Clone ID Related Accession (Same CDS sequence)	XM_013364010.1 , XM_013364010.2
Accession Version	XM_013364010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGGATA CAGACATCCC TCACCAGTGC CAGCCTGGGG 
TCAGCAGACG AGAACTCCAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCATCTGGAG 
CTCACAGAAA CGTGCTTGGA TATGATGGCC CGATACGTGT TCTCCAACTT CACGGCAGTA 
CCTAAAAGGT CTCCTGTGGG AGAATTTCTC CTGGCAGGCG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC AGTGACGACC AGTGTGGGAG CTGGGACCCG CTCATTGCTG 
GGCCTGGACT CTGGGGATCT GCAGGCCAGC CCAGAGTCAA GCTCCAACCC TGGCCTGCAC 
GTGAGACAGA CAAAAGAGGC GCCTGCTAAG CTGGAGTCCC AGACTGGGCA GCAGGTGTCT 
CGCGGGGCCC GGGATCGGGT GCGCTCCATG TCGGGGGGAC ATGGCCTTCG AGTTGCTGCC 
CTGGATGCAC CAGCTTCCCA TATCCCAGGA GGCTGCACTT CTGCAGGACC ACAGAATGTA 
CCAGCTACAA AGCCGGAGAA GGCCTCTGTG GGTGCCCAGG TTCCAGCACC AGAGAAGACG 
AATCTGGCAG CCTATGTACC CCTGCTGACC CAGGGCTGGG CCGAGATCTT GGTCCGAAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTTGAGAACC CACTTAGTCC CTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAACGCCC TCATGGCTGC TGAGCGCTTC 
AAGGAGCACC GTGACACGGC TCTGTACAAG TCACTGTCAG TGCCGGCAGC TGGCACGGCC 
AGGCCCCCTC CTCTCCCACG CTCTAATACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCTGT GGTTCTGGAG 
GAGGGGAGTC CAGGCGAGAC TCATGTACCA GGGGAGCCCC CCGAGTTGGA GGATTTGGAG 
GCAATGCTAA GTGCAGACAG GCGTTGCCGG CGCTCTGAGG CTTACAGCAG GTCATCCTCA 
GCCTCCAGCC AGGAGGAGAA GCCCCACGCT GAGGAGCTGG CTGCGGGGGG CATCCCAATT 
GAGGCAGCAG TCTCCTCTGA GGGTGGCCGG CCCTCCGTGG ACCCCTCCTT CCAGCCATCA 
CAGCCCCTGA GCAAGTCCAG CTCCTCGCCT GAGCTGCAAA CACTGCAGGA CATCCTTGGG 
AACCCTGGGG ACAAGGCTGA TGCTGGCCGG CTGAGCCCTG AGGCCAAGGC CCGATCCCAG 
TCTGGGATCC TGGATGGGGG AAGTGTGGCC TGGGCAGCCC CAGGTGAAGA GGGCCGGGGC 
TCCGCCATAC CCGAGGGTCC TTTGCCATCC AGTTGCCCCC GCTCTCCCGG TGGCCTCCGG 
CCCCGAGGCT ACACCATCTC CGATTCAGCC CCATCGCGCA GGGGCAAGAG GGTGGAGAGG 
GACACTTTGA AGAGCAGAGC TGCAGCCTCC ACTGCTGAGA AAGTGCCTGG CATCAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCACTCG CCATTCTTTG GTGATGAGTC CAACAAGCCC 
ATCCTTTTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAAC TCCTTGACCA GATCCCTTCG 
TATGACACAC ACAAGATTGC TGTGCTGTAT GTGGGCGAAG GCCAGAGCAA CAGCGAGCTG 
GCCATCCTCT CCAATGAGCA CGGCTCCTAC AGGTACACAG AGTTCCTGAC AGGGCTTGGT 
AGGCTCATCG AGCTCAAGGA CTGCCAGCCC GACAAGGTGT ACCTGGGTGG CCTGGACGTG 
TGTGGCGAGG ATGGCCAGTT CACCTACTGT TGGCACGATG ACATCATGCA AGCTGTCTTC 
CATATTGCTA CGCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGA 
CACCTGGGCA ATGACTTTGT CTCCATCATC TACAATGACT CTGGGGAGGA CTTCAAGCTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CACGTGATCA TCACACCCCT GGACTACGAG 
TGCAACCTGC TGTCACTGCA GTGTAGGAAA GACATGGAGG GCCTTGTGGA CACGAGCGTA 
GCCAAGATTG TGTCTGACCG CAACTTGCCC TTTGTGGCTC GCCAGATGGC CCTGCACGCA 
AACATGGCCT CACAGGTACA CCACAGTCGC TCCAACCCCA CCGACATCTA CCCCTCCAAG 
TGGATCGCCC GGCTCCGCCA CATCAAGCGG CTCCGCCACC GGATCCGTGA GGAAGCCCAC 
TACTCGAACC CTAGCCTGCC TCTGCTACAG ATGCAGTCCA CAGCCCACCC CAGAGCCCAG 
GCTCAGGCCT CAGCTGAGGC CACAGCCACC TGTGAGGCTG GCCAGCGGAA GCGCCTCATC 
TCCTCAGTGG ACGACTTCAC CGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219464.1
CDS	112..5544
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364010.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364010.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGGATA CAGACATCCC TCACCAGTGC CAGCCTGGGG 
TCAGCAGACG AGAACTCCAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCATCTGGAG 
CTCACAGAAA CGTGCTTGGA TATGATGGCC CGATACGTGT TCTCCAACTT CACGGCAGTA 
CCTAAAAGGT CTCCTGTGGG AGAATTTCTC CTGGCAGGCG GCAGGACCAA AACCTGGCTG 
GTTGGGAACA AGCTTGTCAC AGTGACGACC AGTGTGGGAG CTGGGACCCG CTCATTGCTG 
GGCCTGGACT CTGGGGATCT GCAGGCCAGC CCAGAGTCAA GCTCCAACCC TGGCCTGCAC 
GTGAGACAGA CAAAAGAGGC GCCTGCTAAG CTGGAGTCCC AGACTGGGCA GCAGGTGTCT 
CGCGGGGCCC GGGATCGGGT GCGCTCCATG TCGGGGGGAC ATGGCCTTCG AGTTGCTGCC 
CTGGATGCAC CAGCTTCCCA TATCCCAGGA GGCTGCACTT CTGCAGGACC ACAGAATGTA 
CCAGCTACAA AGCCGGAGAA GGCCTCTGTG GGTGCCCAGG TTCCAGCACC AGAGAAGACG 
AATCTGGCAG CCTATGTACC CCTGCTGACC CAGGGCTGGG CCGAGATCTT GGTCCGAAGG 
CCCACAGGGA ACACCAGCTG GCTGATGAGC CTTGAGAACC CACTTAGTCC CTTCTCCTCG 
GACATCAACA ACATGCCCCT GCAAGAGCTG TCCAACGCCC TCATGGCTGC TGAGCGCTTC 
AAGGAGCACC GTGACACGGC TCTGTACAAG TCACTGTCAG TGCCGGCAGC TGGCACGGCC 
AGGCCCCCTC CTCTCCCACG CTCTAATACA GTGGCCTCTT TCTCCTCCCT GTACCAGTCC 
AGCTGCCAAG GACAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCTGT GGTTCTGGAG 
GAGGGGAGTC CAGGCGAGAC TCATGTACCA GGGGAGCCCC CCGAGTTGGA GGATTTGGAG 
GCAATGCTAA GTGCAGACAG GCGTTGCCGG CGCTCTGAGG CTTACAGCAG GTCATCCTCA 
GCCTCCAGCC AGGAGGAGAA GCCCCACGCT GAGGAGCTGG CTGCGGGGGG CATCCCAATT 
GAGGCAGCAG TCTCCTCTGA GGGTGGCCGG CCCTCCGTGG ACCCCTCCTT CCAGCCATCA 
CAGCCCCTGA GCAAGTCCAG CTCCTCGCCT GAGCTGCAAA CACTGCAGGA CATCCTTGGG 
AACCCTGGGG ACAAGGCTGA TGCTGGCCGG CTGAGCCCTG AGGCCAAGGC CCGATCCCAG 
TCTGGGATCC TGGATGGGGG AAGTGTGGCC TGGGCAGCCC CAGGTGAAGA GGGCCGGGGC 
TCCGCCATAC CCGAGGGTCC TTTGCCATCC AGTTGCCCCC GCTCTCCCGG TGGCCTCCGG 
CCCCGAGGCT ACACCATCTC CGATTCAGCC CCATCGCGCA GGGGCAAGAG GGTGGAGAGG 
GACACTTTGA AGAGCAGAGC TGCAGCCTCC ACTGCTGAGA AAGTGCCTGG CATCAACCCC 
AGCTTCGTAT TCCTGCAGCT CTACCACTCG CCATTCTTTG GTGATGAGTC CAACAAGCCC 
ATCCTTTTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAAC TCCTTGACCA GATCCCTTCG 
TATGACACAC ACAAGATTGC TGTGCTGTAT GTGGGCGAAG GCCAGAGCAA CAGCGAGCTG 
GCCATCCTCT CCAATGAGCA CGGCTCCTAC AGGTACACAG AGTTCCTGAC AGGGCTTGGT 
AGGCTCATCG AGCTCAAGGA CTGCCAGCCC GACAAGGTGT ACCTGGGTGG CCTGGACGTG 
TGTGGCGAGG ATGGCCAGTT CACCTACTGT TGGCACGATG ACATCATGCA AGCTGTCTTC 
CATATTGCTA CGCTGATGCC CACCAAGGAC GTGGACAAGC ACCGCTGTGA CAAGAAGCGA 
CACCTGGGCA ATGACTTTGT CTCCATCATC TACAATGACT CTGGGGAGGA CTTCAAGCTG 
GGCACCATTA AGGGCCAGTT CAACTTTGTC CACGTGATCA TCACACCCCT GGACTACGAG 
TGCAACCTGC TGTCACTGCA GTGTAGGAAA GACATGGAGG GCCTTGTGGA CACGAGCGTA 
GCCAAGATTG TGTCTGACCG CAACTTGCCC TTTGTGGCTC GCCAGATGGC CCTGCACGCA 
AACATGGCCT CACAGGTACA CCACAGTCGC TCCAACCCCA CCGACATCTA CCCCTCCAAG 
TGGATCGCCC GGCTCCGCCA CATCAAGCGG CTCCGCCACC GGATCCGTGA GGAAGCCCAC 
TACTCGAACC CTAGCCTGCC TCTGCTACAG ATGCAGTCCA CAGCCCACCC CAGAGCCCAG 
GCTCAGGCCT CAGCTGAGGC CACAGCCACC TGTGAGGCTG GCCAGCGGAA GCGCCTCATC 
TCCTCAGTGG ACGACTTCAC CGAGTTTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364011.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219465.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OIc68583
Clone ID Related Accession (Same CDS sequence)	XM_013364011.2 , XM_013364011.1
Accession Version	XM_013364011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219465.1
CDS	112..5478
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364011.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364011.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364011.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219465.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

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CloneID	OIc68583
Clone ID Related Accession (Same CDS sequence)	XM_013364011.2 , XM_013364011.1
Accession Version	XM_013364011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219465.1
CDS	102..5468
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364011.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364011.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364012.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219466.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OIc68584
Clone ID Related Accession (Same CDS sequence)	XM_013364012.2 , XM_013364012.1
Accession Version	XM_013364012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219466.1
CDS	112..5418
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364012.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364012.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364012.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219466.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

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CloneID	OIc68584
Clone ID Related Accession (Same CDS sequence)	XM_013364012.2 , XM_013364012.1
Accession Version	XM_013364012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219466.1
CDS	102..5408
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364012.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364012.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGCTGC 
CAAGGACAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CTGTGGTTCT GGAGGAGGGG 
AGTCCAGGCG AGACTCATGT ACCAGGGGAG CCCCCCGAGT TGGAGGATTT GGAGGCAATG 
CTAAGTGCAG ACAGGCGTTG CCGGCGCTCT GAGGCTTACA GCAGGTCATC CTCAGCCTCC 
AGCCAGGAGG AGAAGCCCCA CGCTGAGGAG CTGGCTGCGG GGGGCATCCC AATTGAGGCA 
GCAGTCTCCT CTGAGGGTGG CCGGCCCTCC GTGGACCCCT CCTTCCAGCC ATCACAGCCC 
CTGAGCAAGT CCAGCTCCTC GCCTGAGCTG CAAACACTGC AGGACATCCT TGGGAACCCT 
GGGGACAAGG CTGATGCTGG CCGGCTGAGC CCTGAGGCCA AGGCCCGATC CCAGTCTGGG 
ATCCTGGATG GGGGAAGTGT GGCCTGGGCA GCCCCAGGTG AAGAGGGCCG GGGCTCCGCC 
ATACCCGAGG GTCCTTTGCC ATCCAGTTGC CCCCGCTCTC CCGGTGGCCT CCGGCCCCGA 
GGCTACACCA TCTCCGATTC AGCCCCATCG CGCAGGGGCA AGAGGGTGGA GAGGGACACT 
TTGAAGAGCA GAGCTGCAGC CTCCACTGCT GAGAAAGTGC CTGGCATCAA CCCCAGCTTC 
GTATTCCTGC AGCTCTACCA CTCGCCATTC TTTGGTGATG AGTCCAACAA GCCCATCCTT 
TTACCCAATG AGTCCTTCGA GCGGTCAGTG CAACTCCTTG ACCAGATCCC TTCGTATGAC 
ACACACAAGA TTGCTGTGCT GTATGTGGGC GAAGGCCAGA GCAACAGCGA GCTGGCCATC 
CTCTCCAATG AGCACGGCTC CTACAGGTAC ACAGAGTTCC TGACAGGGCT TGGTAGGCTC 
ATCGAGCTCA AGGACTGCCA GCCCGACAAG GTGTACCTGG GTGGCCTGGA CGTGTGTGGC 
GAGGATGGCC AGTTCACCTA CTGTTGGCAC GATGACATCA TGCAAGCTGT CTTCCATATT 
GCTACGCTGA TGCCCACCAA GGACGTGGAC AAGCACCGCT GTGACAAGAA GCGACACCTG 
GGCAATGACT TTGTCTCCAT CATCTACAAT GACTCTGGGG AGGACTTCAA GCTGGGCACC 
ATTAAGGGCC AGTTCAACTT TGTCCACGTG ATCATCACAC CCCTGGACTA CGAGTGCAAC 
CTGCTGTCAC TGCAGTGTAG GAAAGACATG GAGGGCCTTG TGGACACGAG CGTAGCCAAG 
ATTGTGTCTG ACCGCAACTT GCCCTTTGTG GCTCGCCAGA TGGCCCTGCA CGCAAACATG 
GCCTCACAGG TACACCACAG TCGCTCCAAC CCCACCGACA TCTACCCCTC CAAGTGGATC 
GCCCGGCTCC GCCACATCAA GCGGCTCCGC CACCGGATCC GTGAGGAAGC CCACTACTCG 
AACCCTAGCC TGCCTCTGCT ACAGATGCAG TCCACAGCCC ACCCCAGAGC CCAGGCTCAG 
GCCTCAGCTG AGGCCACAGC CACCTGTGAG GCTGGCCAGC GGAAGCGCCT CATCTCCTCA 
GTGGACGACT TCACCGAGTT TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364013.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219467.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OIc68585
Clone ID Related Accession (Same CDS sequence)	XM_013364013.2 , XM_013364013.1
Accession Version	XM_013364013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCTG TGGTTCTGGA GGAGGGGAGT 
CCAGGCGAGA CTCATGTACC AGGGGAGCCC CCCGAGTTGG AGGATTTGGA GGCAATGCTA 
AGTGCAGACA GGCGTTGCCG GCGCTCTGAG GCTTACAGCA GGTCATCCTC AGCCTCCAGC 
CAGGAGGAGA AGCCCCACGC TGAGGAGCTG GCTGCGGGGG GCATCCCAAT TGAGGCAGCA 
GTCTCCTCTG AGGGTGGCCG GCCCTCCGTG GACCCCTCCT TCCAGCCATC ACAGCCCCTG 
AGCAAGTCCA GCTCCTCGCC TGAGCTGCAA ACACTGCAGG ACATCCTTGG GAACCCTGGG 
GACAAGGCTG ATGCTGGCCG GCTGAGCCCT GAGGCCAAGG CCCGATCCCA GTCTGGGATC 
CTGGATGGGG GAAGTGTGGC CTGGGCAGCC CCAGGTGAAG AGGGCCGGGG CTCCGCCATA 
CCCGAGGGTC CTTTGCCATC CAGTTGCCCC CGCTCTCCCG GTGGCCTCCG GCCCCGAGGC 
TACACCATCT CCGATTCAGC CCCATCGCGC AGGGGCAAGA GGGTGGAGAG GGACACTTTG 
AAGAGCAGAG CTGCAGCCTC CACTGCTGAG AAAGTGCCTG GCATCAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCACTC GCCATTCTTT GGTGATGAGT CCAACAAGCC CATCCTTTTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAA CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATTG CTGTGCTGTA TGTGGGCGAA GGCCAGAGCA ACAGCGAGCT GGCCATCCTC 
TCCAATGAGC ACGGCTCCTA CAGGTACACA GAGTTCCTGA CAGGGCTTGG TAGGCTCATC 
GAGCTCAAGG ACTGCCAGCC CGACAAGGTG TACCTGGGTG GCCTGGACGT GTGTGGCGAG 
GATGGCCAGT TCACCTACTG TTGGCACGAT GACATCATGC AAGCTGTCTT CCATATTGCT 
ACGCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG ACACCTGGGC 
AATGACTTTG TCTCCATCAT CTACAATGAC TCTGGGGAGG ACTTCAAGCT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCACGTGATC ATCACACCCC TGGACTACGA GTGCAACCTG 
CTGTCACTGC AGTGTAGGAA AGACATGGAG GGCCTTGTGG ACACGAGCGT AGCCAAGATT 
GTGTCTGACC GCAACTTGCC CTTTGTGGCT CGCCAGATGG CCCTGCACGC AAACATGGCC 
TCACAGGTAC ACCACAGTCG CTCCAACCCC ACCGACATCT ACCCCTCCAA GTGGATCGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAC CGGATCCGTG AGGAAGCCCA CTACTCGAAC 
CCTAGCCTGC CTCTGCTACA GATGCAGTCC ACAGCCCACC CCAGAGCCCA GGCTCAGGCC 
TCAGCTGAGG CCACAGCCAC CTGTGAGGCT GGCCAGCGGA AGCGCCTCAT CTCCTCAGTG 
GACGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219467.1
CDS	112..5415
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364013.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364013.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCTG TGGTTCTGGA GGAGGGGAGT 
CCAGGCGAGA CTCATGTACC AGGGGAGCCC CCCGAGTTGG AGGATTTGGA GGCAATGCTA 
AGTGCAGACA GGCGTTGCCG GCGCTCTGAG GCTTACAGCA GGTCATCCTC AGCCTCCAGC 
CAGGAGGAGA AGCCCCACGC TGAGGAGCTG GCTGCGGGGG GCATCCCAAT TGAGGCAGCA 
GTCTCCTCTG AGGGTGGCCG GCCCTCCGTG GACCCCTCCT TCCAGCCATC ACAGCCCCTG 
AGCAAGTCCA GCTCCTCGCC TGAGCTGCAA ACACTGCAGG ACATCCTTGG GAACCCTGGG 
GACAAGGCTG ATGCTGGCCG GCTGAGCCCT GAGGCCAAGG CCCGATCCCA GTCTGGGATC 
CTGGATGGGG GAAGTGTGGC CTGGGCAGCC CCAGGTGAAG AGGGCCGGGG CTCCGCCATA 
CCCGAGGGTC CTTTGCCATC CAGTTGCCCC CGCTCTCCCG GTGGCCTCCG GCCCCGAGGC 
TACACCATCT CCGATTCAGC CCCATCGCGC AGGGGCAAGA GGGTGGAGAG GGACACTTTG 
AAGAGCAGAG CTGCAGCCTC CACTGCTGAG AAAGTGCCTG GCATCAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCACTC GCCATTCTTT GGTGATGAGT CCAACAAGCC CATCCTTTTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAA CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATTG CTGTGCTGTA TGTGGGCGAA GGCCAGAGCA ACAGCGAGCT GGCCATCCTC 
TCCAATGAGC ACGGCTCCTA CAGGTACACA GAGTTCCTGA CAGGGCTTGG TAGGCTCATC 
GAGCTCAAGG ACTGCCAGCC CGACAAGGTG TACCTGGGTG GCCTGGACGT GTGTGGCGAG 
GATGGCCAGT TCACCTACTG TTGGCACGAT GACATCATGC AAGCTGTCTT CCATATTGCT 
ACGCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG ACACCTGGGC 
AATGACTTTG TCTCCATCAT CTACAATGAC TCTGGGGAGG ACTTCAAGCT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCACGTGATC ATCACACCCC TGGACTACGA GTGCAACCTG 
CTGTCACTGC AGTGTAGGAA AGACATGGAG GGCCTTGTGG ACACGAGCGT AGCCAAGATT 
GTGTCTGACC GCAACTTGCC CTTTGTGGCT CGCCAGATGG CCCTGCACGC AAACATGGCC 
TCACAGGTAC ACCACAGTCG CTCCAACCCC ACCGACATCT ACCCCTCCAA GTGGATCGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAC CGGATCCGTG AGGAAGCCCA CTACTCGAAC 
CCTAGCCTGC CTCTGCTACA GATGCAGTCC ACAGCCCACC CCAGAGCCCA GGCTCAGGCC 
TCAGCTGAGG CCACAGCCAC CTGTGAGGCT GGCCAGCGGA AGCGCCTCAT CTCCTCAGTG 
GACGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364013.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219467.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

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CloneID	OIc68585
Clone ID Related Accession (Same CDS sequence)	XM_013364013.2 , XM_013364013.1
Accession Version	XM_013364013.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCTG TGGTTCTGGA GGAGGGGAGT 
CCAGGCGAGA CTCATGTACC AGGGGAGCCC CCCGAGTTGG AGGATTTGGA GGCAATGCTA 
AGTGCAGACA GGCGTTGCCG GCGCTCTGAG GCTTACAGCA GGTCATCCTC AGCCTCCAGC 
CAGGAGGAGA AGCCCCACGC TGAGGAGCTG GCTGCGGGGG GCATCCCAAT TGAGGCAGCA 
GTCTCCTCTG AGGGTGGCCG GCCCTCCGTG GACCCCTCCT TCCAGCCATC ACAGCCCCTG 
AGCAAGTCCA GCTCCTCGCC TGAGCTGCAA ACACTGCAGG ACATCCTTGG GAACCCTGGG 
GACAAGGCTG ATGCTGGCCG GCTGAGCCCT GAGGCCAAGG CCCGATCCCA GTCTGGGATC 
CTGGATGGGG GAAGTGTGGC CTGGGCAGCC CCAGGTGAAG AGGGCCGGGG CTCCGCCATA 
CCCGAGGGTC CTTTGCCATC CAGTTGCCCC CGCTCTCCCG GTGGCCTCCG GCCCCGAGGC 
TACACCATCT CCGATTCAGC CCCATCGCGC AGGGGCAAGA GGGTGGAGAG GGACACTTTG 
AAGAGCAGAG CTGCAGCCTC CACTGCTGAG AAAGTGCCTG GCATCAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCACTC GCCATTCTTT GGTGATGAGT CCAACAAGCC CATCCTTTTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAA CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATTG CTGTGCTGTA TGTGGGCGAA GGCCAGAGCA ACAGCGAGCT GGCCATCCTC 
TCCAATGAGC ACGGCTCCTA CAGGTACACA GAGTTCCTGA CAGGGCTTGG TAGGCTCATC 
GAGCTCAAGG ACTGCCAGCC CGACAAGGTG TACCTGGGTG GCCTGGACGT GTGTGGCGAG 
GATGGCCAGT TCACCTACTG TTGGCACGAT GACATCATGC AAGCTGTCTT CCATATTGCT 
ACGCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG ACACCTGGGC 
AATGACTTTG TCTCCATCAT CTACAATGAC TCTGGGGAGG ACTTCAAGCT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCACGTGATC ATCACACCCC TGGACTACGA GTGCAACCTG 
CTGTCACTGC AGTGTAGGAA AGACATGGAG GGCCTTGTGG ACACGAGCGT AGCCAAGATT 
GTGTCTGACC GCAACTTGCC CTTTGTGGCT CGCCAGATGG CCCTGCACGC AAACATGGCC 
TCACAGGTAC ACCACAGTCG CTCCAACCCC ACCGACATCT ACCCCTCCAA GTGGATCGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAC CGGATCCGTG AGGAAGCCCA CTACTCGAAC 
CCTAGCCTGC CTCTGCTACA GATGCAGTCC ACAGCCCACC CCAGAGCCCA GGCTCAGGCC 
TCAGCTGAGG CCACAGCCAC CTGTGAGGCT GGCCAGCGGA AGCGCCTCAT CTCCTCAGTG 
GACGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219467.1
CDS	102..5405
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364013.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364013.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGCTGCCAA 
GGACAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCTG TGGTTCTGGA GGAGGGGAGT 
CCAGGCGAGA CTCATGTACC AGGGGAGCCC CCCGAGTTGG AGGATTTGGA GGCAATGCTA 
AGTGCAGACA GGCGTTGCCG GCGCTCTGAG GCTTACAGCA GGTCATCCTC AGCCTCCAGC 
CAGGAGGAGA AGCCCCACGC TGAGGAGCTG GCTGCGGGGG GCATCCCAAT TGAGGCAGCA 
GTCTCCTCTG AGGGTGGCCG GCCCTCCGTG GACCCCTCCT TCCAGCCATC ACAGCCCCTG 
AGCAAGTCCA GCTCCTCGCC TGAGCTGCAA ACACTGCAGG ACATCCTTGG GAACCCTGGG 
GACAAGGCTG ATGCTGGCCG GCTGAGCCCT GAGGCCAAGG CCCGATCCCA GTCTGGGATC 
CTGGATGGGG GAAGTGTGGC CTGGGCAGCC CCAGGTGAAG AGGGCCGGGG CTCCGCCATA 
CCCGAGGGTC CTTTGCCATC CAGTTGCCCC CGCTCTCCCG GTGGCCTCCG GCCCCGAGGC 
TACACCATCT CCGATTCAGC CCCATCGCGC AGGGGCAAGA GGGTGGAGAG GGACACTTTG 
AAGAGCAGAG CTGCAGCCTC CACTGCTGAG AAAGTGCCTG GCATCAACCC CAGCTTCGTA 
TTCCTGCAGC TCTACCACTC GCCATTCTTT GGTGATGAGT CCAACAAGCC CATCCTTTTA 
CCCAATGAGT CCTTCGAGCG GTCAGTGCAA CTCCTTGACC AGATCCCTTC GTATGACACA 
CACAAGATTG CTGTGCTGTA TGTGGGCGAA GGCCAGAGCA ACAGCGAGCT GGCCATCCTC 
TCCAATGAGC ACGGCTCCTA CAGGTACACA GAGTTCCTGA CAGGGCTTGG TAGGCTCATC 
GAGCTCAAGG ACTGCCAGCC CGACAAGGTG TACCTGGGTG GCCTGGACGT GTGTGGCGAG 
GATGGCCAGT TCACCTACTG TTGGCACGAT GACATCATGC AAGCTGTCTT CCATATTGCT 
ACGCTGATGC CCACCAAGGA CGTGGACAAG CACCGCTGTG ACAAGAAGCG ACACCTGGGC 
AATGACTTTG TCTCCATCAT CTACAATGAC TCTGGGGAGG ACTTCAAGCT GGGCACCATT 
AAGGGCCAGT TCAACTTTGT CCACGTGATC ATCACACCCC TGGACTACGA GTGCAACCTG 
CTGTCACTGC AGTGTAGGAA AGACATGGAG GGCCTTGTGG ACACGAGCGT AGCCAAGATT 
GTGTCTGACC GCAACTTGCC CTTTGTGGCT CGCCAGATGG CCCTGCACGC AAACATGGCC 
TCACAGGTAC ACCACAGTCG CTCCAACCCC ACCGACATCT ACCCCTCCAA GTGGATCGCC 
CGGCTCCGCC ACATCAAGCG GCTCCGCCAC CGGATCCGTG AGGAAGCCCA CTACTCGAAC 
CCTAGCCTGC CTCTGCTACA GATGCAGTCC ACAGCCCACC CCAGAGCCCA GGCTCAGGCC 
TCAGCTGAGG CCACAGCCAC CTGTGAGGCT GGCCAGCGGA AGCGCCTCAT CTCCTCAGTG 
GACGACTTCA CCGAGTTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364014.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219468.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OIc68586
Clone ID Related Accession (Same CDS sequence)	XM_013364014.1 , XM_013364014.2
Accession Version	XM_013364014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGACTCT GCTGTGGTTC TGGAGGAGGG GAGTCCAGGC 
GAGACTCATG TACCAGGGGA GCCCCCCGAG TTGGAGGATT TGGAGGCAAT GCTAAGTGCA 
GACAGGCGTT GCCGGCGCTC TGAGGCTTAC AGCAGGTCAT CCTCAGCCTC CAGCCAGGAG 
GAGAAGCCCC ACGCTGAGGA GCTGGCTGCG GGGGGCATCC CAATTGAGGC AGCAGTCTCC 
TCTGAGGGTG GCCGGCCCTC CGTGGACCCC TCCTTCCAGC CATCACAGCC CCTGAGCAAG 
TCCAGCTCCT CGCCTGAGCT GCAAACACTG CAGGACATCC TTGGGAACCC TGGGGACAAG 
GCTGATGCTG GCCGGCTGAG CCCTGAGGCC AAGGCCCGAT CCCAGTCTGG GATCCTGGAT 
GGGGGAAGTG TGGCCTGGGC AGCCCCAGGT GAAGAGGGCC GGGGCTCCGC CATACCCGAG 
GGTCCTTTGC CATCCAGTTG CCCCCGCTCT CCCGGTGGCC TCCGGCCCCG AGGCTACACC 
ATCTCCGATT CAGCCCCATC GCGCAGGGGC AAGAGGGTGG AGAGGGACAC TTTGAAGAGC 
AGAGCTGCAG CCTCCACTGC TGAGAAAGTG CCTGGCATCA ACCCCAGCTT CGTATTCCTG 
CAGCTCTACC ACTCGCCATT CTTTGGTGAT GAGTCCAACA AGCCCATCCT TTTACCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAACTCCTT GACCAGATCC CTTCGTATGA CACACACAAG 
ATTGCTGTGC TGTATGTGGG CGAAGGCCAG AGCAACAGCG AGCTGGCCAT CCTCTCCAAT 
GAGCACGGCT CCTACAGGTA CACAGAGTTC CTGACAGGGC TTGGTAGGCT CATCGAGCTC 
AAGGACTGCC AGCCCGACAA GGTGTACCTG GGTGGCCTGG ACGTGTGTGG CGAGGATGGC 
CAGTTCACCT ACTGTTGGCA CGATGACATC ATGCAAGCTG TCTTCCATAT TGCTACGCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGTGACAAGA AGCGACACCT GGGCAATGAC 
TTTGTCTCCA TCATCTACAA TGACTCTGGG GAGGACTTCA AGCTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACA CCCCTGGACT ACGAGTGCAA CCTGCTGTCA 
CTGCAGTGTA GGAAAGACAT GGAGGGCCTT GTGGACACGA GCGTAGCCAA GATTGTGTCT 
GACCGCAACT TGCCCTTTGT GGCTCGCCAG ATGGCCCTGC ACGCAAACAT GGCCTCACAG 
GTACACCACA GTCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT CGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCACCGGATC CGTGAGGAAG CCCACTACTC GAACCCTAGC 
CTGCCTCTGC TACAGATGCA GTCCACAGCC CACCCCAGAG CCCAGGCTCA GGCCTCAGCT 
GAGGCCACAG CCACCTGTGA GGCTGGCCAG CGGAAGCGCC TCATCTCCTC AGTGGACGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219468.1
CDS	102..5339
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364014.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364014.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGACTCT GCTGTGGTTC TGGAGGAGGG GAGTCCAGGC 
GAGACTCATG TACCAGGGGA GCCCCCCGAG TTGGAGGATT TGGAGGCAAT GCTAAGTGCA 
GACAGGCGTT GCCGGCGCTC TGAGGCTTAC AGCAGGTCAT CCTCAGCCTC CAGCCAGGAG 
GAGAAGCCCC ACGCTGAGGA GCTGGCTGCG GGGGGCATCC CAATTGAGGC AGCAGTCTCC 
TCTGAGGGTG GCCGGCCCTC CGTGGACCCC TCCTTCCAGC CATCACAGCC CCTGAGCAAG 
TCCAGCTCCT CGCCTGAGCT GCAAACACTG CAGGACATCC TTGGGAACCC TGGGGACAAG 
GCTGATGCTG GCCGGCTGAG CCCTGAGGCC AAGGCCCGAT CCCAGTCTGG GATCCTGGAT 
GGGGGAAGTG TGGCCTGGGC AGCCCCAGGT GAAGAGGGCC GGGGCTCCGC CATACCCGAG 
GGTCCTTTGC CATCCAGTTG CCCCCGCTCT CCCGGTGGCC TCCGGCCCCG AGGCTACACC 
ATCTCCGATT CAGCCCCATC GCGCAGGGGC AAGAGGGTGG AGAGGGACAC TTTGAAGAGC 
AGAGCTGCAG CCTCCACTGC TGAGAAAGTG CCTGGCATCA ACCCCAGCTT CGTATTCCTG 
CAGCTCTACC ACTCGCCATT CTTTGGTGAT GAGTCCAACA AGCCCATCCT TTTACCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAACTCCTT GACCAGATCC CTTCGTATGA CACACACAAG 
ATTGCTGTGC TGTATGTGGG CGAAGGCCAG AGCAACAGCG AGCTGGCCAT CCTCTCCAAT 
GAGCACGGCT CCTACAGGTA CACAGAGTTC CTGACAGGGC TTGGTAGGCT CATCGAGCTC 
AAGGACTGCC AGCCCGACAA GGTGTACCTG GGTGGCCTGG ACGTGTGTGG CGAGGATGGC 
CAGTTCACCT ACTGTTGGCA CGATGACATC ATGCAAGCTG TCTTCCATAT TGCTACGCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGTGACAAGA AGCGACACCT GGGCAATGAC 
TTTGTCTCCA TCATCTACAA TGACTCTGGG GAGGACTTCA AGCTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACA CCCCTGGACT ACGAGTGCAA CCTGCTGTCA 
CTGCAGTGTA GGAAAGACAT GGAGGGCCTT GTGGACACGA GCGTAGCCAA GATTGTGTCT 
GACCGCAACT TGCCCTTTGT GGCTCGCCAG ATGGCCCTGC ACGCAAACAT GGCCTCACAG 
GTACACCACA GTCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT CGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCACCGGATC CGTGAGGAAG CCCACTACTC GAACCCTAGC 
CTGCCTCTGC TACAGATGCA GTCCACAGCC CACCCCAGAG CCCAGGCTCA GGCCTCAGCT 
GAGGCCACAG CCACCTGTGA GGCTGGCCAG CGGAAGCGCC TCATCTCCTC AGTGGACGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364014.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219468.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

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CloneID	OIc68586
Clone ID Related Accession (Same CDS sequence)	XM_013364014.1 , XM_013364014.2
Accession Version	XM_013364014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGACTCT GCTGTGGTTC TGGAGGAGGG GAGTCCAGGC 
GAGACTCATG TACCAGGGGA GCCCCCCGAG TTGGAGGATT TGGAGGCAAT GCTAAGTGCA 
GACAGGCGTT GCCGGCGCTC TGAGGCTTAC AGCAGGTCAT CCTCAGCCTC CAGCCAGGAG 
GAGAAGCCCC ACGCTGAGGA GCTGGCTGCG GGGGGCATCC CAATTGAGGC AGCAGTCTCC 
TCTGAGGGTG GCCGGCCCTC CGTGGACCCC TCCTTCCAGC CATCACAGCC CCTGAGCAAG 
TCCAGCTCCT CGCCTGAGCT GCAAACACTG CAGGACATCC TTGGGAACCC TGGGGACAAG 
GCTGATGCTG GCCGGCTGAG CCCTGAGGCC AAGGCCCGAT CCCAGTCTGG GATCCTGGAT 
GGGGGAAGTG TGGCCTGGGC AGCCCCAGGT GAAGAGGGCC GGGGCTCCGC CATACCCGAG 
GGTCCTTTGC CATCCAGTTG CCCCCGCTCT CCCGGTGGCC TCCGGCCCCG AGGCTACACC 
ATCTCCGATT CAGCCCCATC GCGCAGGGGC AAGAGGGTGG AGAGGGACAC TTTGAAGAGC 
AGAGCTGCAG CCTCCACTGC TGAGAAAGTG CCTGGCATCA ACCCCAGCTT CGTATTCCTG 
CAGCTCTACC ACTCGCCATT CTTTGGTGAT GAGTCCAACA AGCCCATCCT TTTACCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAACTCCTT GACCAGATCC CTTCGTATGA CACACACAAG 
ATTGCTGTGC TGTATGTGGG CGAAGGCCAG AGCAACAGCG AGCTGGCCAT CCTCTCCAAT 
GAGCACGGCT CCTACAGGTA CACAGAGTTC CTGACAGGGC TTGGTAGGCT CATCGAGCTC 
AAGGACTGCC AGCCCGACAA GGTGTACCTG GGTGGCCTGG ACGTGTGTGG CGAGGATGGC 
CAGTTCACCT ACTGTTGGCA CGATGACATC ATGCAAGCTG TCTTCCATAT TGCTACGCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGTGACAAGA AGCGACACCT GGGCAATGAC 
TTTGTCTCCA TCATCTACAA TGACTCTGGG GAGGACTTCA AGCTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACA CCCCTGGACT ACGAGTGCAA CCTGCTGTCA 
CTGCAGTGTA GGAAAGACAT GGAGGGCCTT GTGGACACGA GCGTAGCCAA GATTGTGTCT 
GACCGCAACT TGCCCTTTGT GGCTCGCCAG ATGGCCCTGC ACGCAAACAT GGCCTCACAG 
GTACACCACA GTCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT CGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCACCGGATC CGTGAGGAAG CCCACTACTC GAACCCTAGC 
CTGCCTCTGC TACAGATGCA GTCCACAGCC CACCCCAGAG CCCAGGCTCA GGCCTCAGCT 
GAGGCCACAG CCACCTGTGA GGCTGGCCAG CGGAAGCGCC TCATCTCCTC AGTGGACGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219468.1
CDS	112..5349
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364014.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364014.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGCAG GATACAGACA TCCCTCACCA GTGCCAGCCT GGGGTCAGCA 
GACGAGAACT CCATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCATCT GGAGCTCACA 
GAAACGTGCT TGGATATGAT GGCCCGATAC GTGTTCTCCA ACTTCACGGC AGTACCTAAA 
AGGTCTCCTG TGGGAGAATT TCTCCTGGCA GGCGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACAGTGAC GACCAGTGTG GGAGCTGGGA CCCGCTCATT GCTGGGCCTG 
GACTCTGGGG ATCTGCAGGC CAGCCCAGAG TCAAGCTCCA ACCCTGGCCT GCACGTGAGA 
CAGACAAAAG AGGCGCCTGC TAAGCTGGAG TCCCAGACTG GGCAGCAGGT GTCTCGCGGG 
GCCCGGGATC GGGTGCGCTC CATGTCGGGG GGACATGGCC TTCGAGTTGC TGCCCTGGAT 
GCACCAGCTT CCCATATCCC AGGAGGCTGC ACTTCTGCAG GACCACAGAA TGTACCAGCT 
ACAAAGCCGG AGAAGGCCTC TGTGGGTGCC CAGGTTCCAG CACCAGAGAA GACGAATCTG 
GCAGCCTATG TACCCCTGCT GACCCAGGGC TGGGCCGAGA TCTTGGTCCG AAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTTGAG AACCCACTTA GTCCCTTCTC CTCGGACATC 
AACAACATGC CCCTGCAAGA GCTGTCCAAC GCCCTCATGG CTGCTGAGCG CTTCAAGGAG 
CACCGTGACA CGGCTCTGTA CAAGTCACTG TCAGTGCCGG CAGCTGGCAC GGCCAGGCCC 
CCTCCTCTCC CACGCTCTAA TACAGACTCT GCTGTGGTTC TGGAGGAGGG GAGTCCAGGC 
GAGACTCATG TACCAGGGGA GCCCCCCGAG TTGGAGGATT TGGAGGCAAT GCTAAGTGCA 
GACAGGCGTT GCCGGCGCTC TGAGGCTTAC AGCAGGTCAT CCTCAGCCTC CAGCCAGGAG 
GAGAAGCCCC ACGCTGAGGA GCTGGCTGCG GGGGGCATCC CAATTGAGGC AGCAGTCTCC 
TCTGAGGGTG GCCGGCCCTC CGTGGACCCC TCCTTCCAGC CATCACAGCC CCTGAGCAAG 
TCCAGCTCCT CGCCTGAGCT GCAAACACTG CAGGACATCC TTGGGAACCC TGGGGACAAG 
GCTGATGCTG GCCGGCTGAG CCCTGAGGCC AAGGCCCGAT CCCAGTCTGG GATCCTGGAT 
GGGGGAAGTG TGGCCTGGGC AGCCCCAGGT GAAGAGGGCC GGGGCTCCGC CATACCCGAG 
GGTCCTTTGC CATCCAGTTG CCCCCGCTCT CCCGGTGGCC TCCGGCCCCG AGGCTACACC 
ATCTCCGATT CAGCCCCATC GCGCAGGGGC AAGAGGGTGG AGAGGGACAC TTTGAAGAGC 
AGAGCTGCAG CCTCCACTGC TGAGAAAGTG CCTGGCATCA ACCCCAGCTT CGTATTCCTG 
CAGCTCTACC ACTCGCCATT CTTTGGTGAT GAGTCCAACA AGCCCATCCT TTTACCCAAT 
GAGTCCTTCG AGCGGTCAGT GCAACTCCTT GACCAGATCC CTTCGTATGA CACACACAAG 
ATTGCTGTGC TGTATGTGGG CGAAGGCCAG AGCAACAGCG AGCTGGCCAT CCTCTCCAAT 
GAGCACGGCT CCTACAGGTA CACAGAGTTC CTGACAGGGC TTGGTAGGCT CATCGAGCTC 
AAGGACTGCC AGCCCGACAA GGTGTACCTG GGTGGCCTGG ACGTGTGTGG CGAGGATGGC 
CAGTTCACCT ACTGTTGGCA CGATGACATC ATGCAAGCTG TCTTCCATAT TGCTACGCTG 
ATGCCCACCA AGGACGTGGA CAAGCACCGC TGTGACAAGA AGCGACACCT GGGCAATGAC 
TTTGTCTCCA TCATCTACAA TGACTCTGGG GAGGACTTCA AGCTGGGCAC CATTAAGGGC 
CAGTTCAACT TTGTCCACGT GATCATCACA CCCCTGGACT ACGAGTGCAA CCTGCTGTCA 
CTGCAGTGTA GGAAAGACAT GGAGGGCCTT GTGGACACGA GCGTAGCCAA GATTGTGTCT 
GACCGCAACT TGCCCTTTGT GGCTCGCCAG ATGGCCCTGC ACGCAAACAT GGCCTCACAG 
GTACACCACA GTCGCTCCAA CCCCACCGAC ATCTACCCCT CCAAGTGGAT CGCCCGGCTC 
CGCCACATCA AGCGGCTCCG CCACCGGATC CGTGAGGAAG CCCACTACTC GAACCCTAGC 
CTGCCTCTGC TACAGATGCA GTCCACAGCC CACCCCAGAG CCCAGGCTCA GGCCTCAGCT 
GAGGCCACAG CCACCTGTGA GGCTGGCCAG CGGAAGCGCC TCATCTCCTC AGTGGACGAC 
TTCACCGAGT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364015.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219469.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.

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CloneID	OIc68587
Clone ID Related Accession (Same CDS sequence)	XM_013364015.1 , XM_013364015.2
Accession Version	XM_013364015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGACTCTGCT GTGGTTCTGG AGGAGGGGAG TCCAGGCGAG 
ACTCATGTAC CAGGGGAGCC CCCCGAGTTG GAGGATTTGG AGGCAATGCT AAGTGCAGAC 
AGGCGTTGCC GGCGCTCTGA GGCTTACAGC AGGTCATCCT CAGCCTCCAG CCAGGAGGAG 
AAGCCCCACG CTGAGGAGCT GGCTGCGGGG GGCATCCCAA TTGAGGCAGC AGTCTCCTCT 
GAGGGTGGCC GGCCCTCCGT GGACCCCTCC TTCCAGCCAT CACAGCCCCT GAGCAAGTCC 
AGCTCCTCGC CTGAGCTGCA AACACTGCAG GACATCCTTG GGAACCCTGG GGACAAGGCT 
GATGCTGGCC GGCTGAGCCC TGAGGCCAAG GCCCGATCCC AGTCTGGGAT CCTGGATGGG 
GGAAGTGTGG CCTGGGCAGC CCCAGGTGAA GAGGGCCGGG GCTCCGCCAT ACCCGAGGGT 
CCTTTGCCAT CCAGTTGCCC CCGCTCTCCC GGTGGCCTCC GGCCCCGAGG CTACACCATC 
TCCGATTCAG CCCCATCGCG CAGGGGCAAG AGGGTGGAGA GGGACACTTT GAAGAGCAGA 
GCTGCAGCCT CCACTGCTGA GAAAGTGCCT GGCATCAACC CCAGCTTCGT ATTCCTGCAG 
CTCTACCACT CGCCATTCTT TGGTGATGAG TCCAACAAGC CCATCCTTTT ACCCAATGAG 
TCCTTCGAGC GGTCAGTGCA ACTCCTTGAC CAGATCCCTT CGTATGACAC ACACAAGATT 
GCTGTGCTGT ATGTGGGCGA AGGCCAGAGC AACAGCGAGC TGGCCATCCT CTCCAATGAG 
CACGGCTCCT ACAGGTACAC AGAGTTCCTG ACAGGGCTTG GTAGGCTCAT CGAGCTCAAG 
GACTGCCAGC CCGACAAGGT GTACCTGGGT GGCCTGGACG TGTGTGGCGA GGATGGCCAG 
TTCACCTACT GTTGGCACGA TGACATCATG CAAGCTGTCT TCCATATTGC TACGCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GACACCTGGG CAATGACTTT 
GTCTCCATCA TCTACAATGA CTCTGGGGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CATCACACCC CTGGACTACG AGTGCAACCT GCTGTCACTG 
CAGTGTAGGA AAGACATGGA GGGCCTTGTG GACACGAGCG TAGCCAAGAT TGTGTCTGAC 
CGCAACTTGC CCTTTGTGGC TCGCCAGATG GCCCTGCACG CAAACATGGC CTCACAGGTA 
CACCACAGTC GCTCCAACCC CACCGACATC TACCCCTCCA AGTGGATCGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA CCGGATCCGT GAGGAAGCCC ACTACTCGAA CCCTAGCCTG 
CCTCTGCTAC AGATGCAGTC CACAGCCCAC CCCAGAGCCC AGGCTCAGGC CTCAGCTGAG 
GCCACAGCCA CCTGTGAGGC TGGCCAGCGG AAGCGCCTCA TCTCCTCAGT GGACGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219469.1
CDS	102..5336
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364015.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364015.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGACTCTGCT GTGGTTCTGG AGGAGGGGAG TCCAGGCGAG 
ACTCATGTAC CAGGGGAGCC CCCCGAGTTG GAGGATTTGG AGGCAATGCT AAGTGCAGAC 
AGGCGTTGCC GGCGCTCTGA GGCTTACAGC AGGTCATCCT CAGCCTCCAG CCAGGAGGAG 
AAGCCCCACG CTGAGGAGCT GGCTGCGGGG GGCATCCCAA TTGAGGCAGC AGTCTCCTCT 
GAGGGTGGCC GGCCCTCCGT GGACCCCTCC TTCCAGCCAT CACAGCCCCT GAGCAAGTCC 
AGCTCCTCGC CTGAGCTGCA AACACTGCAG GACATCCTTG GGAACCCTGG GGACAAGGCT 
GATGCTGGCC GGCTGAGCCC TGAGGCCAAG GCCCGATCCC AGTCTGGGAT CCTGGATGGG 
GGAAGTGTGG CCTGGGCAGC CCCAGGTGAA GAGGGCCGGG GCTCCGCCAT ACCCGAGGGT 
CCTTTGCCAT CCAGTTGCCC CCGCTCTCCC GGTGGCCTCC GGCCCCGAGG CTACACCATC 
TCCGATTCAG CCCCATCGCG CAGGGGCAAG AGGGTGGAGA GGGACACTTT GAAGAGCAGA 
GCTGCAGCCT CCACTGCTGA GAAAGTGCCT GGCATCAACC CCAGCTTCGT ATTCCTGCAG 
CTCTACCACT CGCCATTCTT TGGTGATGAG TCCAACAAGC CCATCCTTTT ACCCAATGAG 
TCCTTCGAGC GGTCAGTGCA ACTCCTTGAC CAGATCCCTT CGTATGACAC ACACAAGATT 
GCTGTGCTGT ATGTGGGCGA AGGCCAGAGC AACAGCGAGC TGGCCATCCT CTCCAATGAG 
CACGGCTCCT ACAGGTACAC AGAGTTCCTG ACAGGGCTTG GTAGGCTCAT CGAGCTCAAG 
GACTGCCAGC CCGACAAGGT GTACCTGGGT GGCCTGGACG TGTGTGGCGA GGATGGCCAG 
TTCACCTACT GTTGGCACGA TGACATCATG CAAGCTGTCT TCCATATTGC TACGCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GACACCTGGG CAATGACTTT 
GTCTCCATCA TCTACAATGA CTCTGGGGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CATCACACCC CTGGACTACG AGTGCAACCT GCTGTCACTG 
CAGTGTAGGA AAGACATGGA GGGCCTTGTG GACACGAGCG TAGCCAAGAT TGTGTCTGAC 
CGCAACTTGC CCTTTGTGGC TCGCCAGATG GCCCTGCACG CAAACATGGC CTCACAGGTA 
CACCACAGTC GCTCCAACCC CACCGACATC TACCCCTCCA AGTGGATCGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA CCGGATCCGT GAGGAAGCCC ACTACTCGAA CCCTAGCCTG 
CCTCTGCTAC AGATGCAGTC CACAGCCCAC CCCAGAGCCC AGGCTCAGGC CTCAGCTGAG 
GCCACAGCCA CCTGTGAGGC TGGCCAGCGG AAGCGCCTCA TCTCCTCAGT GGACGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364015.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219469.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.

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CloneID	OIc68587
Clone ID Related Accession (Same CDS sequence)	XM_013364015.1 , XM_013364015.2
Accession Version	XM_013364015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGACTCTGCT GTGGTTCTGG AGGAGGGGAG TCCAGGCGAG 
ACTCATGTAC CAGGGGAGCC CCCCGAGTTG GAGGATTTGG AGGCAATGCT AAGTGCAGAC 
AGGCGTTGCC GGCGCTCTGA GGCTTACAGC AGGTCATCCT CAGCCTCCAG CCAGGAGGAG 
AAGCCCCACG CTGAGGAGCT GGCTGCGGGG GGCATCCCAA TTGAGGCAGC AGTCTCCTCT 
GAGGGTGGCC GGCCCTCCGT GGACCCCTCC TTCCAGCCAT CACAGCCCCT GAGCAAGTCC 
AGCTCCTCGC CTGAGCTGCA AACACTGCAG GACATCCTTG GGAACCCTGG GGACAAGGCT 
GATGCTGGCC GGCTGAGCCC TGAGGCCAAG GCCCGATCCC AGTCTGGGAT CCTGGATGGG 
GGAAGTGTGG CCTGGGCAGC CCCAGGTGAA GAGGGCCGGG GCTCCGCCAT ACCCGAGGGT 
CCTTTGCCAT CCAGTTGCCC CCGCTCTCCC GGTGGCCTCC GGCCCCGAGG CTACACCATC 
TCCGATTCAG CCCCATCGCG CAGGGGCAAG AGGGTGGAGA GGGACACTTT GAAGAGCAGA 
GCTGCAGCCT CCACTGCTGA GAAAGTGCCT GGCATCAACC CCAGCTTCGT ATTCCTGCAG 
CTCTACCACT CGCCATTCTT TGGTGATGAG TCCAACAAGC CCATCCTTTT ACCCAATGAG 
TCCTTCGAGC GGTCAGTGCA ACTCCTTGAC CAGATCCCTT CGTATGACAC ACACAAGATT 
GCTGTGCTGT ATGTGGGCGA AGGCCAGAGC AACAGCGAGC TGGCCATCCT CTCCAATGAG 
CACGGCTCCT ACAGGTACAC AGAGTTCCTG ACAGGGCTTG GTAGGCTCAT CGAGCTCAAG 
GACTGCCAGC CCGACAAGGT GTACCTGGGT GGCCTGGACG TGTGTGGCGA GGATGGCCAG 
TTCACCTACT GTTGGCACGA TGACATCATG CAAGCTGTCT TCCATATTGC TACGCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GACACCTGGG CAATGACTTT 
GTCTCCATCA TCTACAATGA CTCTGGGGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CATCACACCC CTGGACTACG AGTGCAACCT GCTGTCACTG 
CAGTGTAGGA AAGACATGGA GGGCCTTGTG GACACGAGCG TAGCCAAGAT TGTGTCTGAC 
CGCAACTTGC CCTTTGTGGC TCGCCAGATG GCCCTGCACG CAAACATGGC CTCACAGGTA 
CACCACAGTC GCTCCAACCC CACCGACATC TACCCCTCCA AGTGGATCGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA CCGGATCCGT GAGGAAGCCC ACTACTCGAA CCCTAGCCTG 
CCTCTGCTAC AGATGCAGTC CACAGCCCAC CCCAGAGCCC AGGCTCAGGC CTCAGCTGAG 
GCCACAGCCA CCTGTGAGGC TGGCCAGCGG AAGCGCCTCA TCTCCTCAGT GGACGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219469.1
CDS	112..5346
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTDSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_013364015.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364015.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGGAT ACAGACATCC CTCACCAGTG CCAGCCTGGG GTCAGCAGAC 
GAGAACTCCA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCATCTGGA GCTCACAGAA 
ACGTGCTTGG ATATGATGGC CCGATACGTG TTCTCCAACT TCACGGCAGT ACCTAAAAGG 
TCTCCTGTGG GAGAATTTCT CCTGGCAGGC GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CAGTGACGAC CAGTGTGGGA GCTGGGACCC GCTCATTGCT GGGCCTGGAC 
TCTGGGGATC TGCAGGCCAG CCCAGAGTCA AGCTCCAACC CTGGCCTGCA CGTGAGACAG 
ACAAAAGAGG CGCCTGCTAA GCTGGAGTCC CAGACTGGGC AGCAGGTGTC TCGCGGGGCC 
CGGGATCGGG TGCGCTCCAT GTCGGGGGGA CATGGCCTTC GAGTTGCTGC CCTGGATGCA 
CCAGCTTCCC ATATCCCAGG AGGCTGCACT TCTGCAGGAC CACAGAATGT ACCAGCTACA 
AAGCCGGAGA AGGCCTCTGT GGGTGCCCAG GTTCCAGCAC CAGAGAAGAC GAATCTGGCA 
GCCTATGTAC CCCTGCTGAC CCAGGGCTGG GCCGAGATCT TGGTCCGAAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTTGAGAAC CCACTTAGTC CCTTCTCCTC GGACATCAAC 
AACATGCCCC TGCAAGAGCT GTCCAACGCC CTCATGGCTG CTGAGCGCTT CAAGGAGCAC 
CGTGACACGG CTCTGTACAA GTCACTGTCA GTGCCGGCAG CTGGCACGGC CAGGCCCCCT 
CCTCTCCCAC GCTCTAATAC AGACTCTGCT GTGGTTCTGG AGGAGGGGAG TCCAGGCGAG 
ACTCATGTAC CAGGGGAGCC CCCCGAGTTG GAGGATTTGG AGGCAATGCT AAGTGCAGAC 
AGGCGTTGCC GGCGCTCTGA GGCTTACAGC AGGTCATCCT CAGCCTCCAG CCAGGAGGAG 
AAGCCCCACG CTGAGGAGCT GGCTGCGGGG GGCATCCCAA TTGAGGCAGC AGTCTCCTCT 
GAGGGTGGCC GGCCCTCCGT GGACCCCTCC TTCCAGCCAT CACAGCCCCT GAGCAAGTCC 
AGCTCCTCGC CTGAGCTGCA AACACTGCAG GACATCCTTG GGAACCCTGG GGACAAGGCT 
GATGCTGGCC GGCTGAGCCC TGAGGCCAAG GCCCGATCCC AGTCTGGGAT CCTGGATGGG 
GGAAGTGTGG CCTGGGCAGC CCCAGGTGAA GAGGGCCGGG GCTCCGCCAT ACCCGAGGGT 
CCTTTGCCAT CCAGTTGCCC CCGCTCTCCC GGTGGCCTCC GGCCCCGAGG CTACACCATC 
TCCGATTCAG CCCCATCGCG CAGGGGCAAG AGGGTGGAGA GGGACACTTT GAAGAGCAGA 
GCTGCAGCCT CCACTGCTGA GAAAGTGCCT GGCATCAACC CCAGCTTCGT ATTCCTGCAG 
CTCTACCACT CGCCATTCTT TGGTGATGAG TCCAACAAGC CCATCCTTTT ACCCAATGAG 
TCCTTCGAGC GGTCAGTGCA ACTCCTTGAC CAGATCCCTT CGTATGACAC ACACAAGATT 
GCTGTGCTGT ATGTGGGCGA AGGCCAGAGC AACAGCGAGC TGGCCATCCT CTCCAATGAG 
CACGGCTCCT ACAGGTACAC AGAGTTCCTG ACAGGGCTTG GTAGGCTCAT CGAGCTCAAG 
GACTGCCAGC CCGACAAGGT GTACCTGGGT GGCCTGGACG TGTGTGGCGA GGATGGCCAG 
TTCACCTACT GTTGGCACGA TGACATCATG CAAGCTGTCT TCCATATTGC TACGCTGATG 
CCCACCAAGG ACGTGGACAA GCACCGCTGT GACAAGAAGC GACACCTGGG CAATGACTTT 
GTCTCCATCA TCTACAATGA CTCTGGGGAG GACTTCAAGC TGGGCACCAT TAAGGGCCAG 
TTCAACTTTG TCCACGTGAT CATCACACCC CTGGACTACG AGTGCAACCT GCTGTCACTG 
CAGTGTAGGA AAGACATGGA GGGCCTTGTG GACACGAGCG TAGCCAAGAT TGTGTCTGAC 
CGCAACTTGC CCTTTGTGGC TCGCCAGATG GCCCTGCACG CAAACATGGC CTCACAGGTA 
CACCACAGTC GCTCCAACCC CACCGACATC TACCCCTCCA AGTGGATCGC CCGGCTCCGC 
CACATCAAGC GGCTCCGCCA CCGGATCCGT GAGGAAGCCC ACTACTCGAA CCCTAGCCTG 
CCTCTGCTAC AGATGCAGTC CACAGCCCAC CCCAGAGCCC AGGCTCAGGC CTCAGCTGAG 
GCCACAGCCA CCTGTGAGGC TGGCCAGCGG AAGCGCCTCA TCTCCTCAGT GGACGACTTC 
ACCGAGTTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364016.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219470.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.

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CloneID	OIc68588
Clone ID Related Accession (Same CDS sequence)	XM_013364016.2 , XM_013364016.1
Accession Version	XM_013364016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013364016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGATGGCC CTGCACGCAA ACATGGCCTC 
ACAGGTACAC CACAGTCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATCGCCCG 
GCTCCGCCAC ATCAAGCGGC TCCGCCACCG GATCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219470.1
CDS	112..5310
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDGPARKHGLTGTPQSLQPHRHLPLQVDRPAPPHQAAPPPDP

Target ORF information:

RefSeq Version	XM_013364016.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364016.2

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGATGGCC CTGCACGCAA ACATGGCCTC 
ACAGGTACAC CACAGTCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATCGCCCG 
GCTCCGCCAC ATCAAGCGGC TCCGCCACCG GATCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_013364016.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_013219470.1 Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.

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CloneID	OIc68588
Clone ID Related Accession (Same CDS sequence)	XM_013364016.2 , XM_013364016.1
Accession Version	XM_013364016.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	tuberin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGATGGCC CTGCACGCAA ACATGGCCTC 
ACAGGTACAC CACAGTCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATCGCCCG 
GCTCCGCCAC ATCAAGCGGC TCCGCCACCG GATCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219470.1
CDS	102..5300
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDGPARKHGLTGTPQSLQPHRHLPLQVDRPAPPHQAAPPPDP

Target ORF information:

RefSeq Version	XM_013364016.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus tuberous sclerosis 2 (Tsc2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013364016.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGAG CAACAGCGAG 
CTGGCCATCC TCTCCAATGA GCACGGCTCC TACAGGTACA CAGAGTTCCT GACAGGGCTT 
GGTAGGCTCA TCGAGCTCAA GGACTGCCAG CCCGACAAGG TGTACCTGGG TGGCCTGGAC 
GTGTGTGGCG AGGATGGCCA GTTCACCTAC TGTTGGCACG ATGACATCAT GCAAGCTGTC 
TTCCATATTG CTACGCTGAT GCCCACCAAG GACGTGGACA AGCACCGCTG TGACAAGAAG 
CGACACCTGG GCAATGACTT TGTCTCCATC ATCTACAATG ACTCTGGGGA GGACTTCAAG 
CTGGGCACCA TTAAGGGCCA GTTCAACTTT GTCCACGTGA TCATCACACC CCTGGACTAC 
GAGTGCAACC TGCTGTCACT GCAGTGTAGG AAAGATGGCC CTGCACGCAA ACATGGCCTC 
ACAGGTACAC CACAGTCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATCGCCCG 
GCTCCGCCAC ATCAAGCGGC TCCGCCACCG GATCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_021734944.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_021590619.1 Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

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CloneID	OIc84028
Clone ID Related Accession (Same CDS sequence)	XM_021734944.1
Accession Version	XM_021734944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	tuberin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936694.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGTT CACCTACTGT 
TGGCACGATG ACATCATGCA AGCTGTCTTC CATATTGCTA CGCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGA CACCTGGGCA ATGACTTTGT CTCCATCATC 
TACAATGACT CTGGGGAGGA CTTCAAGCTG GGCACCATTA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACACCCCT GGACTACGAG TGCAACCTGC TGTCACTGCA GTGTAGGAAA 
GACATGGAGG GCCTTGTGGA CACGAGCGTA GCCAAGATTG TGTCTGACCG CAACTTGCCC 
TTTGTGGCTC GCCAGATGGC CCTGCACGCA AACATGGCCT CACAGGTACA CCACAGTCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATCGCCC GGCTCCGCCA CATCAAGCGG 
CTCCGCCACC GGATCCGTGA GGAAGCCCAC TACTCGAACC CTAGCCTGCC TCTGCTACAG 
ATGCAGTCCA CAGCCCACCC CAGAGCCCAG GCTCAGGCCT CAGCTGAGGC CACAGCCACC 
TGTGAGGCTG GCCAGCGGAA GCGCCTCATC TCCTCAGTGG ACGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021590619.1
CDS	112..5394
Translation	MAKPASKDSGLKEKFKILLGLGTPRPSPRSAEGKQTEFIITAEILRELSVECGLNNRIRVIGQICEVAKTKKFEEHAVEALWKTVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGKHITYLEEELAEFVLQWMDVGLTSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLNIIERLLQQLQSLESPELRTIVHDLLTTVEELCDQNEFHGSQERYFQLVESCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNEPRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARPLSLPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHAMRVYETLVSHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGIVRFSPYCLCDCAEPEWGSEKKASGPLSPPTGPSGTVPSGPTVRLGHLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCNVDQLSSALCSMLSGPKTLERLRGTPEGFSRTDLHLAVVPVLTALVSYHSYLDKAKQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGSNNSPPVKELKESPAAEAFRCRSVSVSDHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGAGTRSLLGLDSGDLQASPESSSNPGLHVRQTKEAPAKLESQTGQQVSRGARDRVRSMSGGHGLRVAALDAPASHIPGGCTSAGPQNVPATKPEKASVGAQVPAPEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGTARPPPLPRSNTVASFSSLYQSSCQGQLHRSISWADSAVVLEEGSPGETHVPGEPPELEDLEAMLSADRRCRRSEAYSRSSSASSQEEKPHAEELAAGGIPIEAAVSSEGGRPSVDPSFQPSQPLSKSSSSPELQTLQDILGNPGDKADAGRLSPEAKARSQSGILDGGSVAWAAPGEEGRGSAIPEGPLPSSCPRSPGGLRPRGYTISDSAPSRRGKRVERDTLKSRAAASTAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYECNLLSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREEAHYSNPSLPLLQMQSTAHPRAQAQASAEATATCEAGQRKRLISSVDDFTEFV

Target ORF information:

RefSeq Version	XM_021734944.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus TSC complex subunit 2 (Tsc2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734944.1

ORF Insert Sequence:

ATGGCCAAGC CAGCAAGCAA AGATTCAGGC TTGAAGGAAA AGTTCAAGAT TCTGCTGGGA 
CTGGGAACAC CAAGGCCAAG CCCTAGATCC GCAGAAGGCA AACAGACGGA GTTTATCATC 
ACAGCGGAAA TCCTGAGAGA ACTGAGTGTT GAATGTGGTC TCAACAATCG CATCAGAGTG 
ATAGGGCAGA TCTGTGAAGT TGCAAAAACT AAGAAGTTTG AAGAGCATGC AGTGGAAGCA 
CTCTGGAAGA CTGTCGCGGA CCTGCTGCAG CCAGAGCGGC CACCAGAGGC TCGACACGCG 
GTGCTGGCTC TGCTGAAGGC CATTGTGCAG GGGCAGGGTG ACCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAT CCCTCCAATG AAGACCTCCA TGAAAGGCTG 
GAAGTTTTCA AGGCCCTTAC GGACAATGGG AAACACATCA CCTACTTGGA AGAAGAACTG 
GCGGAGTTTG TCCTGCAGTG GATGGATGTT GGCTTGACCT CAGAATTCCT TCTGGTGCTT 
GTGAACTTGG TCAAATTTAA CAGCTGTTAC CTCGATGAGT ATATTGCATC AATGGTTCAC 
ATGATCTGTC TGCTCTGCAT CCGGACTGTG TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTATAACTGC CTGCCGGCTG AAAGCCTCCC CCTGTTCATC 
GTCACTCTTT GTCGTACTAT CAACGTCAAG GAGCTCTGTG AGCCTTGTTG GAAGCTGATG 
CGTAACCTCC TGGGCACCCA CCTGGGCCAT AGTGCCATCT ACAACATGTG CCGCATCATG 
GAAGACAGAG CCTATATGGA AGATGCCCCA CTGCTGCGAG GAGCTGTGTT CTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGACTCTAT TCTCTCAAGA ACTCCCCAAC ATCTGTGTTG 
CCATCTTTTT ATGAGGCCAT GACCTGTCCC AATGAGGTGG TGTCCTATGA GATCGTGCTG 
TCCATAACCA GACTTATCAA GAAGTACAGA AAGGAGCTCC AGGCTGTGAC GTGGGACATC 
CTGCTGAACA TCATCGAGAG GCTGCTTCAG CAGCTCCAGA GCTTGGAGAG CCCAGAGCTC 
AGAACCATCG TCCATGACCT GCTGACCACT GTGGAGGAGC TGTGTGACCA GAATGAGTTC 
CATGGCTCAC AGGAGAGATA CTTCCAGCTG GTGGAGAGCT GTGCAGACCA GAGGCCCGAG 
TCCTCCCTCT TAAACCTGAT ATCCTATCGA GCTCAGTCCA TCCACCCAGC AAAGGATGGC 
TGGATCCAGA ATCTGCAGCT GCTGATGGAG AGGTTCTTCA GGAACGAGCC CCGCAGTGCT 
GTGCGCATCA AGGTGCTGGA TGTGCTGTCC TTTGTGCTGC TTATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC GGTGGTCATC TCCCAACTTT CCCACATCCC TGAGGATAAA 
GATCACCAAG TCCGAAAGCT GGCTACCCAA TTGCTGGTGG ACCTGGCGGA AGGCTGCCAC 
ACCCACCACT TCAACAGCCT GCTGGATATC ATCGAAAAAG TGATGGCCCG CCCCCTTTCC 
CTGCCTCCTG AGCTGGAAGA AAGGGATGTA GCTGCCTACT CGGCCTCCCT GGAGGATGTG 
AAGACTGCAG TCCTGGGGCT TCTGGTCATT CTTCAGACCA AGCTGTATAC CTTGCCTGCC 
AGCCATGCCA TGCGTGTGTA TGAGACGCTT GTCAGCCACA TTCAGCTCCA CTACAAGCAT 
GGCTACTCCC TGCCCATTGC CAGTAGCATC CGGCTACAGG CCTTCGACTT CCTGCTGCTA 
CTGCGGGCTG ATTCACTGCA CCGCCTCGGG TTGCCCAGCA AGGATGGGAT CGTGCGGTTC 
AGCCCCTACT GCCTCTGTGA CTGCGCGGAG CCAGAGTGGG GCTCTGAGAA GAAGGCCAGC 
GGCCCCCTTT CACCTCCCAC TGGGCCATCT GGCACTGTCC CCTCTGGCCC TACCGTGCGG 
CTTGGCCACC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGTTT GAAGCAGGAG 
ACTGACTGGA AGGTGTTGAA GCTAGTCCTC AGCAAGCTGC CTGAGTCGCT GCGCTACAAG 
GTCCTCATCT TCACCTCCCC ATGCAATGTC GACCAACTGT CATCTGCCCT CTGCTCCATG 
CTCTCAGGTC CAAAGACCCT CGAGCGGCTC CGAGGCACCC CCGAAGGCTT CTCCAGAACC 
GACCTCCACT TGGCCGTGGT CCCGGTGCTG ACAGCTCTGG TCTCTTATCA CAGCTACTTA 
GACAAAGCCA AGCAGCGTGA GATGGTTTAC TGCCTGGAGC AAGGCCTCAT CTACCGCTGT 
GCCAGCCAGT GTGTGGTGGC CCTAGCCATC TGCAGCGTGG AGATGCCCGA CATCATCATC 
AAGGCGCTGC CTGTCCTGGT GGTGAAGCTG ACACACATCT CTGCCACGGC CAGCATGGCT 
GTGCCGCTCC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CCCATCTCTA CAGAAACTTT 
GCTGCAGAGC AGTACGCAAG TGTGTTTGCC ATCTCCCTGC CGTACACTAA CCCCTCCAAG 
TTCAACCAGT ACATCGTGTG CCTGGCCCAT CATGTTATTG CCATGTGGTT CATTAGGTGT 
CGCCTGCCCT TCCGGAAGGA TTTTGTCCCT TACATCACTA AGGGCCTGCG CTCTAACGTC 
CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGGAG CACCAGTCTC 
AACGAGAGGC CCAAAAGTTT GAGGATAGCC AGAGCCCCCA AACAAGGCTC GAATAACTCC 
CCACCTGTGA AAGAACTCAA GGAGAGCCCT GCAGCCGAGG CCTTCCGGTG CCGCAGCGTC 
AGTGTGTCTG ATCATGTGGT CCGCAGCAGG ATACAGACAT CCCTCACCAG TGCCAGCCTG 
GGGTCAGCAG ACGAGAACTC CATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCATCTG 
GAGCTCACAG AAACGTGCTT GGATATGATG GCCCGATACG TGTTCTCCAA CTTCACGGCA 
GTACCTAAAA GGTCTCCTGT GGGAGAATTT CTCCTGGCAG GCGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACAGTGACG ACCAGTGTGG GAGCTGGGAC CCGCTCATTG 
CTGGGCCTGG ACTCTGGGGA TCTGCAGGCC AGCCCAGAGT CAAGCTCCAA CCCTGGCCTG 
CACGTGAGAC AGACAAAAGA GGCGCCTGCT AAGCTGGAGT CCCAGACTGG GCAGCAGGTG 
TCTCGCGGGG CCCGGGATCG GGTGCGCTCC ATGTCGGGGG GACATGGCCT TCGAGTTGCT 
GCCCTGGATG CACCAGCTTC CCATATCCCA GGAGGCTGCA CTTCTGCAGG ACCACAGAAT 
GTACCAGCTA CAAAGCCGGA GAAGGCCTCT GTGGGTGCCC AGGTTCCAGC ACCAGAGAAG 
ACGAATCTGG CAGCCTATGT ACCCCTGCTG ACCCAGGGCT GGGCCGAGAT CTTGGTCCGA 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTTGAGA ACCCACTTAG TCCCTTCTCC 
TCGGACATCA ACAACATGCC CCTGCAAGAG CTGTCCAACG CCCTCATGGC TGCTGAGCGC 
TTCAAGGAGC ACCGTGACAC GGCTCTGTAC AAGTCACTGT CAGTGCCGGC AGCTGGCACG 
GCCAGGCCCC CTCCTCTCCC ACGCTCTAAT ACAGTGGCCT CTTTCTCCTC CCTGTACCAG 
TCCAGCTGCC AAGGACAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC TGTGGTTCTG 
GAGGAGGGGA GTCCAGGCGA GACTCATGTA CCAGGGGAGC CCCCCGAGTT GGAGGATTTG 
GAGGCAATGC TAAGTGCAGA CAGGCGTTGC CGGCGCTCTG AGGCTTACAG CAGGTCATCC 
TCAGCCTCCA GCCAGGAGGA GAAGCCCCAC GCTGAGGAGC TGGCTGCGGG GGGCATCCCA 
ATTGAGGCAG CAGTCTCCTC TGAGGGTGGC CGGCCCTCCG TGGACCCCTC CTTCCAGCCA 
TCACAGCCCC TGAGCAAGTC CAGCTCCTCG CCTGAGCTGC AAACACTGCA GGACATCCTT 
GGGAACCCTG GGGACAAGGC TGATGCTGGC CGGCTGAGCC CTGAGGCCAA GGCCCGATCC 
CAGTCTGGGA TCCTGGATGG GGGAAGTGTG GCCTGGGCAG CCCCAGGTGA AGAGGGCCGG 
GGCTCCGCCA TACCCGAGGG TCCTTTGCCA TCCAGTTGCC CCCGCTCTCC CGGTGGCCTC 
CGGCCCCGAG GCTACACCAT CTCCGATTCA GCCCCATCGC GCAGGGGCAA GAGGGTGGAG 
AGGGACACTT TGAAGAGCAG AGCTGCAGCC TCCACTGCTG AGAAAGTGCC TGGCATCAAC 
CCCAGCTTCG TATTCCTGCA GCTCTACCAC TCGCCATTCT TTGGTGATGA GTCCAACAAG 
CCCATCCTTT TACCCAATGA GTCCTTCGAG CGGTCAGTGC AACTCCTTGA CCAGATCCCT 
TCGTATGACA CACACAAGAT TGCTGTGCTG TATGTGGGCG AAGGCCAGTT CACCTACTGT 
TGGCACGATG ACATCATGCA AGCTGTCTTC CATATTGCTA CGCTGATGCC CACCAAGGAC 
GTGGACAAGC ACCGCTGTGA CAAGAAGCGA CACCTGGGCA ATGACTTTGT CTCCATCATC 
TACAATGACT CTGGGGAGGA CTTCAAGCTG GGCACCATTA AGGGCCAGTT CAACTTTGTC 
CACGTGATCA TCACACCCCT GGACTACGAG TGCAACCTGC TGTCACTGCA GTGTAGGAAA 
GACATGGAGG GCCTTGTGGA CACGAGCGTA GCCAAGATTG TGTCTGACCG CAACTTGCCC 
TTTGTGGCTC GCCAGATGGC CCTGCACGCA AACATGGCCT CACAGGTACA CCACAGTCGC 
TCCAACCCCA CCGACATCTA CCCCTCCAAG TGGATCGCCC GGCTCCGCCA CATCAAGCGG 
CTCCGCCACC GGATCCGTGA GGAAGCCCAC TACTCGAACC CTAGCCTGCC TCTGCTACAG 
ATGCAGTCCA CAGCCCACCC CAGAGCCCAG GCTCAGGCCT CAGCTGAGGC CACAGCCACC 
TGTGAGGCTG GCCAGCGGAA GCGCCTCATC TCCTCAGTGG ACGACTTCAC CGAGTTTGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC2
Entrez Gene ID	101967417
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC2 cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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