Nrap gene cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA	Protein	Name
XM_005320686.3	XP_005320743.1	nebulin-related-anchoring protein isoform X1
XM_013356570.1	XP_013212024.1	nebulin-related-anchoring protein isoform X3
XM_021723330.1	XP_021579005.1	nebulin-related-anchoring protein isoform X5
XM_013356570.2	XP_013212024.1	nebulin-related-anchoring protein isoform X6
XM_021723329.1	XP_021579004.1	nebulin-related-anchoring protein isoform X4
XM_013356569.1	XP_013212023.1	nebulin-related-anchoring protein isoform X2
XM_005320686.2	XP_005320743.1	nebulin-related-anchoring protein isoform X1
XM_013356569.2	XP_013212023.1	nebulin-related-anchoring protein isoform X3
XM_021723328.1	XP_021579003.1	nebulin-related-anchoring protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc52001	XM_005320686.3 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc52002	XM_013356569.2 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc52003	XM_013356570.2 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc76024	XM_021723328.1 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc76025	XM_021723329.1 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc76026	XM_021723330.1 Latest version!	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OIc52003	XM_013356570.1	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc52001	XM_005320686.2	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc52002	XM_013356569.1	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Nrap ( XM_005320686.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005320743.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc52001
Clone ID Related Accession (Same CDS sequence)	XM_005320686.3 , XM_005320686.2
Accession Version	XM_005320686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005320686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTGGAATATA AGAAGGATCT GGAAAGTAGT AAAGGTCACA GTATCAACTA CTGTGAAACG 
CCTCAGTTCA GGAACGTGAG CAAGATCTCA AAATTCACCA GTGATAATAA ATATAAAGAA 
AACTACCAGA ACCACATGAG AGGCCACTAT GAAGGAGTTG GCATGGACAG ACGCACACTT 
CATGCTCTGA AAGTTGGCAG TCTGGCCAGC AATGTTGCCT ACAAGGCTGA TTACAAACAC 
GATATTGTGG ACTACAACTA TCCAGCCACC CTTACTCCTG CCTATCAAAC AACAATGAAA 
CTGGTTCCTT TGAAAGATGT CAACTACAGG CAAAGCATCG ACAAGCTGAA GTACAGCTCT 
GTGACCAATA CTCCGCAGAT TGTTCAGGCC AAAATCAATG CCCAGCAACT GAGCCACGTG 
AACTACCGCG CTGACTATGA GAAAAACAAG TTGAATTACA CACTGCCGCA GGATGTGCCT 
CAGCTGGTGA AGGCCAGAAC TAACGCTGAA CTCTTCAGTG AGGTTAAGTA CAAAGAAGGC 
TGGGAGAAGA CAAAGGGGAA AGGATTTGAG ATGAAGCTGG ATGCCATGTC TCTGTTGGCC 
GCCAAAGCCT CGGGGGAGCT TGCTAGCAAT ATTAAATACA AAGAAGAATA TGAGAAAACA 
AAAGGCAAAG CCATGGGAAC AACCGACTCG AAGCTTCTGC ACTCCCTGCA GGTGGCCAAG 
ATGAGCAGCG AGGTTGAATA CAAGAAAGGC TTTGAGGAGA GTAAGACCCA CTTTCACCTG 
CCCATGGATA TGGTAAACAT CAGGCATGCT AAGAAGGCCC AAGCTCTTGC CAGTGACCTG 
GATTACAGGA AGAAACTGCA CGAATACACC GTGCTGCCTG AAGACATGAA GACTCAGTGG 
GCCAAGAAAG CCTACGGGCT CCAGAGCGAG CTGCAGTACA AATCCGACCT GGCGTGGATG 
AAGGGAGTCG GGTGGCTGAC TGAGGGCAGT CTCAATTTGG AACAGGCCAA GAAGGCTGGA 
CAACTGGTCA GCGAGAAAAA CTACCGGCAG AGGGTTGATG AGCTGAAGTT CACCAGTGTG 
ACCGATAGTG CGCAGATGGA GCACGCCAAG AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC 
TACAAGGCAG AGAACCAGCA GTCTATCCAC CAGTACACCA TCAGCAAAGA CGAGCCTCTC 
TTCCTGCAGG CCCGGGCCAA TGCTGCTCAC CTCAGCGAGA AACGGTACAA GAGCAGCTGG 
GAAAACCAGA AGGCAAGAGG GTTTGAGCTG AGTCTTGATT CCTTGACCTT CCTGGCAGCC 
AAAGCCAAGA GGGACCTGGC GAGCGAGGTG AAGTACAAGG AGGACTATGA GAAGTCCAGA 
GGGAAGCTCA TCGGGGCCAA GGGTGCACAG GGCGATTCTC AAATGAGCCA CTCGCTGCAA 
ATGTCCAAAC TGCAGAGCGA ATTGGAGTAC AAAAAGGGCT TCGAAGACAC CAAGTCTCAG 
TGCCACGTCT CCCTCGACAT GATCCACCTG GCGCACGCCC GCCAGGCTCA GCACTTAGCC 
ACAGACATAG GCTACAGAAC AGTGTCCCAT CACTTCACAG CGTTGCCCAC GGACATGAAG 
GTGGAGTGGG CCAAGAAGGC GTATGGCTTG CAGAGTGATA ACCAATACAG GGCAGATGTG 
AAGTGGATGA AAGGCACGGG CTGGGTCGCC ACCGGGTCAT TAAATGTGGA GCAGGCAAAG 
AAGGCAGGAG AACTCATTAG CGAGAAGAAG TACCGTCAGC ATCCAGATGC TTTGAAGTTT 
ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG 
GATAGGCTGT ACAGGGAACA AGGAGAGAAC ATAAAGCATC ATTACACGCA GACCACAGAC 
CTGCCTGAGG TCCTGCTGGC CAAGCTGAAT GCCATGAACA TCAGCGAGAC ACGGTATAAG 
GAATCCTGGA GTAAACTTCG AGACGGTGGA TATAAACTAA GGTTGGATGC CATTCCGTTC 
CAGGCAGCAA AGGCTTCTAG TGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG 
AAAATGAAAG GACAGATGCT TGGCTCCCGG AGCTTGGAAG ATGATCTCAG CCTTGCACAT 
TCAGTGCACG CGACCTCACT GCAGAGTGAT GTGAATTACA AGAAAGGCTT TGAACACTCA 
AAGGCACACT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCACGCCAA GAAGGCTCAG 
ACCCTGGCCA GCGACCAGGA CTACAGACAT CCGCTCCCCC AGCACACGTC CTTGGCAGAA 
GACCTGAGGC TGCGCTGTGC CAAGAAAGCT CACAAGTTGC AGAGCGAGAA TTTGTACCGC 
TCAGACCTGA ACTTCATGCG AGGCGTTGCC TGCGTCGTTC CAGGCACGTT GGAGATTGAA 
GGAAGAAAGA GGGCATCTGA GCTCATCAGT GAGAGTAAAT ACCGTCAGCA TCCTGGGTCG 
ATCAAGTACA CGACTGTGAC GGACACCCCC AACCTCCTTC ATGCAAAGTT CAGCAACCAG 
ATTACCAATG AGCGTCTCTA TAAAGCAGCT GGAGAGGATG CTAGACACCA GTACACAATG 
ACCCTGGGTC TGCCCGAGTT TGTCCGAGCA AAAACAAATG CGGCCAATCT GAGCGACGCA 
AAATACAAGG AGTCTTGGCA TGATCTCCGT GCTCAAGGCT ACAAGCTGAC AATAGATGCG 
CTCCCCTTCC AGGCTGCAAG GGCCTCTGGG GACATCGCCA GCGATTTTCT CTACAGACAC 
GACTTTGTGA AGGAGCGAGG GAAGCTCATC GGGATCCGCA GCGTGAGCGA TGACCCCCGG 
CTGCGGCACT GTCGGCTCAT GGGCCAGCTG CAAAGTGAGC ATCAGTACAA GAGGGAGGCA 
GCCGGCAGCC AAGCCCAGTG CCACCTACCC ATGGACATGG TGCACCTGGT CCATGCCAGG 
AAGGCCCAGG CCCTGGCCAG TGACCATGAC TACAGGACTC AGTACCACGA GTTCACGGCG 
CTACCCGAAG ACTTGAAGTT GGCCTGGGCC AAGAAAGCCC ACGCCCTGCA GAGTGAGTTT 
CGCTACAAGT CGGACCTCAT GGCCATGAAG GGGACAGGAT GGCTAGCACT GAGATCCCCA 
CAGATAGAGA GCGCCAGGAA GGCGGGAGAA CTCATCAGCG AGACCAAATA CCGTAAAAAA 
CCAGACAGTA TCAAGTTCAC CACAGTGGTT GACTCCCCAG ATCTGGTTCA CGCCAAGAAT 
AGCTACGTGC ATTGCAACGA GCGCCTGTAC AGGTTGGGGG ATGCAGAGGC CCTGCACAGA 
TACACCCTGA TCCCCGACCA CCCTGAGTTC ACCCGAGCCC GCCTCAACGC GCTGCACCTG 
AGTGACAAAG TCTACAGAAA CTCCTGGGAG CAGACCCGCG CTGGCGGCTA CGACTTCAGG 
CTGGACGCCA TCCCGTTCCA GACTGCCCGG GCATCCAGGG AGATCGCCAG CGACTTCCGG 
TATAAAGAGG CTTTCCTGCG GGACCGGGGC CTGCAGATCG GCTTCCGCAG CATCAACGAT 
GACCCGAGGA TGAAGCATTT CCTGAACGTC GGCAGGCTCC AGAGTGACAA TGAGTACAAG 
AAGGACTTTG CCAAGAGTCG GTCCCAGTTC CACAGCCGCA CAGACCAGCC TGGCTTCCTC 
CAGGCCAAGA GGAGCCAGCA GTTGGCCAGC GACGTGCATT ACCGGCAGCC CCTGCCCCAG 
CCCACGTGCG ACCCCGAGCA GCTGGGCCTC AAGCACGCGC AGAAGGCCCA CCAGCTGCAG 
AGTGACGTCA AGTACAAATC AGACTTGAAC CTGACCAGGG GTGTCGGCTG GACCCCTCCC 
GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAC TGGCCAACAT GAGGGGCCTG 
GGGCTCCCGG GGGCTTATGG GGAGCCGGAG GTGGTGGAGG CTGGAGGTCA CCCGAGGGGG 
GAGGTGAACC CAGATGCCAC TGAGATTCTG CACGTCAAAA GGAAAAAGGC TCTGTGGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005320743.1
CDS	237..5399
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_005320686.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005320686.3

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTGGAATATA AGAAGGATCT GGAAAGTAGT AAAGGTCACA GTATCAACTA CTGTGAAACG 
CCTCAGTTCA GGAACGTGAG CAAGATCTCA AAATTCACCA GTGATAATAA ATATAAAGAA 
AACTACCAGA ACCACATGAG AGGCCACTAT GAAGGAGTTG GCATGGACAG ACGCACACTT 
CATGCTCTGA AAGTTGGCAG TCTGGCCAGC AATGTTGCCT ACAAGGCTGA TTACAAACAC 
GATATTGTGG ACTACAACTA TCCAGCCACC CTTACTCCTG CCTATCAAAC AACAATGAAA 
CTGGTTCCTT TGAAAGATGT CAACTACAGG CAAAGCATCG ACAAGCTGAA GTACAGCTCT 
GTGACCAATA CTCCGCAGAT TGTTCAGGCC AAAATCAATG CCCAGCAACT GAGCCACGTG 
AACTACCGCG CTGACTATGA GAAAAACAAG TTGAATTACA CACTGCCGCA GGATGTGCCT 
CAGCTGGTGA AGGCCAGAAC TAACGCTGAA CTCTTCAGTG AGGTTAAGTA CAAAGAAGGC 
TGGGAGAAGA CAAAGGGGAA AGGATTTGAG ATGAAGCTGG ATGCCATGTC TCTGTTGGCC 
GCCAAAGCCT CGGGGGAGCT TGCTAGCAAT ATTAAATACA AAGAAGAATA TGAGAAAACA 
AAAGGCAAAG CCATGGGAAC AACCGACTCG AAGCTTCTGC ACTCCCTGCA GGTGGCCAAG 
ATGAGCAGCG AGGTTGAATA CAAGAAAGGC TTTGAGGAGA GTAAGACCCA CTTTCACCTG 
CCCATGGATA TGGTAAACAT CAGGCATGCT AAGAAGGCCC AAGCTCTTGC CAGTGACCTG 
GATTACAGGA AGAAACTGCA CGAATACACC GTGCTGCCTG AAGACATGAA GACTCAGTGG 
GCCAAGAAAG CCTACGGGCT CCAGAGCGAG CTGCAGTACA AATCCGACCT GGCGTGGATG 
AAGGGAGTCG GGTGGCTGAC TGAGGGCAGT CTCAATTTGG AACAGGCCAA GAAGGCTGGA 
CAACTGGTCA GCGAGAAAAA CTACCGGCAG AGGGTTGATG AGCTGAAGTT CACCAGTGTG 
ACCGATAGTG CGCAGATGGA GCACGCCAAG AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC 
TACAAGGCAG AGAACCAGCA GTCTATCCAC CAGTACACCA TCAGCAAAGA CGAGCCTCTC 
TTCCTGCAGG CCCGGGCCAA TGCTGCTCAC CTCAGCGAGA AACGGTACAA GAGCAGCTGG 
GAAAACCAGA AGGCAAGAGG GTTTGAGCTG AGTCTTGATT CCTTGACCTT CCTGGCAGCC 
AAAGCCAAGA GGGACCTGGC GAGCGAGGTG AAGTACAAGG AGGACTATGA GAAGTCCAGA 
GGGAAGCTCA TCGGGGCCAA GGGTGCACAG GGCGATTCTC AAATGAGCCA CTCGCTGCAA 
ATGTCCAAAC TGCAGAGCGA ATTGGAGTAC AAAAAGGGCT TCGAAGACAC CAAGTCTCAG 
TGCCACGTCT CCCTCGACAT GATCCACCTG GCGCACGCCC GCCAGGCTCA GCACTTAGCC 
ACAGACATAG GCTACAGAAC AGTGTCCCAT CACTTCACAG CGTTGCCCAC GGACATGAAG 
GTGGAGTGGG CCAAGAAGGC GTATGGCTTG CAGAGTGATA ACCAATACAG GGCAGATGTG 
AAGTGGATGA AAGGCACGGG CTGGGTCGCC ACCGGGTCAT TAAATGTGGA GCAGGCAAAG 
AAGGCAGGAG AACTCATTAG CGAGAAGAAG TACCGTCAGC ATCCAGATGC TTTGAAGTTT 
ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG 
GATAGGCTGT ACAGGGAACA AGGAGAGAAC ATAAAGCATC ATTACACGCA GACCACAGAC 
CTGCCTGAGG TCCTGCTGGC CAAGCTGAAT GCCATGAACA TCAGCGAGAC ACGGTATAAG 
GAATCCTGGA GTAAACTTCG AGACGGTGGA TATAAACTAA GGTTGGATGC CATTCCGTTC 
CAGGCAGCAA AGGCTTCTAG TGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG 
AAAATGAAAG GACAGATGCT TGGCTCCCGG AGCTTGGAAG ATGATCTCAG CCTTGCACAT 
TCAGTGCACG CGACCTCACT GCAGAGTGAT GTGAATTACA AGAAAGGCTT TGAACACTCA 
AAGGCACACT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCACGCCAA GAAGGCTCAG 
ACCCTGGCCA GCGACCAGGA CTACAGACAT CCGCTCCCCC AGCACACGTC CTTGGCAGAA 
GACCTGAGGC TGCGCTGTGC CAAGAAAGCT CACAAGTTGC AGAGCGAGAA TTTGTACCGC 
TCAGACCTGA ACTTCATGCG AGGCGTTGCC TGCGTCGTTC CAGGCACGTT GGAGATTGAA 
GGAAGAAAGA GGGCATCTGA GCTCATCAGT GAGAGTAAAT ACCGTCAGCA TCCTGGGTCG 
ATCAAGTACA CGACTGTGAC GGACACCCCC AACCTCCTTC ATGCAAAGTT CAGCAACCAG 
ATTACCAATG AGCGTCTCTA TAAAGCAGCT GGAGAGGATG CTAGACACCA GTACACAATG 
ACCCTGGGTC TGCCCGAGTT TGTCCGAGCA AAAACAAATG CGGCCAATCT GAGCGACGCA 
AAATACAAGG AGTCTTGGCA TGATCTCCGT GCTCAAGGCT ACAAGCTGAC AATAGATGCG 
CTCCCCTTCC AGGCTGCAAG GGCCTCTGGG GACATCGCCA GCGATTTTCT CTACAGACAC 
GACTTTGTGA AGGAGCGAGG GAAGCTCATC GGGATCCGCA GCGTGAGCGA TGACCCCCGG 
CTGCGGCACT GTCGGCTCAT GGGCCAGCTG CAAAGTGAGC ATCAGTACAA GAGGGAGGCA 
GCCGGCAGCC AAGCCCAGTG CCACCTACCC ATGGACATGG TGCACCTGGT CCATGCCAGG 
AAGGCCCAGG CCCTGGCCAG TGACCATGAC TACAGGACTC AGTACCACGA GTTCACGGCG 
CTACCCGAAG ACTTGAAGTT GGCCTGGGCC AAGAAAGCCC ACGCCCTGCA GAGTGAGTTT 
CGCTACAAGT CGGACCTCAT GGCCATGAAG GGGACAGGAT GGCTAGCACT GAGATCCCCA 
CAGATAGAGA GCGCCAGGAA GGCGGGAGAA CTCATCAGCG AGACCAAATA CCGTAAAAAA 
CCAGACAGTA TCAAGTTCAC CACAGTGGTT GACTCCCCAG ATCTGGTTCA CGCCAAGAAT 
AGCTACGTGC ATTGCAACGA GCGCCTGTAC AGGTTGGGGG ATGCAGAGGC CCTGCACAGA 
TACACCCTGA TCCCCGACCA CCCTGAGTTC ACCCGAGCCC GCCTCAACGC GCTGCACCTG 
AGTGACAAAG TCTACAGAAA CTCCTGGGAG CAGACCCGCG CTGGCGGCTA CGACTTCAGG 
CTGGACGCCA TCCCGTTCCA GACTGCCCGG GCATCCAGGG AGATCGCCAG CGACTTCCGG 
TATAAAGAGG CTTTCCTGCG GGACCGGGGC CTGCAGATCG GCTTCCGCAG CATCAACGAT 
GACCCGAGGA TGAAGCATTT CCTGAACGTC GGCAGGCTCC AGAGTGACAA TGAGTACAAG 
AAGGACTTTG CCAAGAGTCG GTCCCAGTTC CACAGCCGCA CAGACCAGCC TGGCTTCCTC 
CAGGCCAAGA GGAGCCAGCA GTTGGCCAGC GACGTGCATT ACCGGCAGCC CCTGCCCCAG 
CCCACGTGCG ACCCCGAGCA GCTGGGCCTC AAGCACGCGC AGAAGGCCCA CCAGCTGCAG 
AGTGACGTCA AGTACAAATC AGACTTGAAC CTGACCAGGG GTGTCGGCTG GACCCCTCCC 
GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAC TGGCCAACAT GAGGGGCCTG 
GGGCTCCCGG GGGCTTATGG GGAGCCGGAG GTGGTGGAGG CTGGAGGTCA CCCGAGGGGG 
GAGGTGAACC CAGATGCCAC TGAGATTCTG CACGTCAAAA GGAAAAAGGC TCTGTGGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_005320686.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005320743.1 Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X1, mRNA.

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CloneID	OIc52001
Clone ID Related Accession (Same CDS sequence)	XM_005320686.3 , XM_005320686.2
Accession Version	XM_005320686.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005320686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTGGAATATA AGAAGGATCT GGAAAGTAGT AAAGGTCACA GTATCAACTA CTGTGAAACG 
CCTCAGTTCA GGAACGTGAG CAAGATCTCA AAATTCACCA GTGATAATAA ATATAAAGAA 
AACTACCAGA ACCACATGAG AGGCCACTAT GAAGGAGTTG GCATGGACAG ACGCACACTT 
CATGCTCTGA AAGTTGGCAG TCTGGCCAGC AATGTTGCCT ACAAGGCTGA TTACAAACAC 
GATATTGTGG ACTACAACTA TCCAGCCACC CTTACTCCTG CCTATCAAAC AACAATGAAA 
CTGGTTCCTT TGAAAGATGT CAACTACAGG CAAAGCATCG ACAAGCTGAA GTACAGCTCT 
GTGACCAATA CTCCGCAGAT TGTTCAGGCC AAAATCAATG CCCAGCAACT GAGCCACGTG 
AACTACCGCG CTGACTATGA GAAAAACAAG TTGAATTACA CACTGCCGCA GGATGTGCCT 
CAGCTGGTGA AGGCCAGAAC TAACGCTGAA CTCTTCAGTG AGGTTAAGTA CAAAGAAGGC 
TGGGAGAAGA CAAAGGGGAA AGGATTTGAG ATGAAGCTGG ATGCCATGTC TCTGTTGGCC 
GCCAAAGCCT CGGGGGAGCT TGCTAGCAAT ATTAAATACA AAGAAGAATA TGAGAAAACA 
AAAGGCAAAG CCATGGGAAC AACCGACTCG AAGCTTCTGC ACTCCCTGCA GGTGGCCAAG 
ATGAGCAGCG AGGTTGAATA CAAGAAAGGC TTTGAGGAGA GTAAGACCCA CTTTCACCTG 
CCCATGGATA TGGTAAACAT CAGGCATGCT AAGAAGGCCC AAGCTCTTGC CAGTGACCTG 
GATTACAGGA AGAAACTGCA CGAATACACC GTGCTGCCTG AAGACATGAA GACTCAGTGG 
GCCAAGAAAG CCTACGGGCT CCAGAGCGAG CTGCAGTACA AATCCGACCT GGCGTGGATG 
AAGGGAGTCG GGTGGCTGAC TGAGGGCAGT CTCAATTTGG AACAGGCCAA GAAGGCTGGA 
CAACTGGTCA GCGAGAAAAA CTACCGGCAG AGGGTTGATG AGCTGAAGTT CACCAGTGTG 
ACCGATAGTG CGCAGATGGA GCACGCCAAG AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC 
TACAAGGCAG AGAACCAGCA GTCTATCCAC CAGTACACCA TCAGCAAAGA CGAGCCTCTC 
TTCCTGCAGG CCCGGGCCAA TGCTGCTCAC CTCAGCGAGA AACGGTACAA GAGCAGCTGG 
GAAAACCAGA AGGCAAGAGG GTTTGAGCTG AGTCTTGATT CCTTGACCTT CCTGGCAGCC 
AAAGCCAAGA GGGACCTGGC GAGCGAGGTG AAGTACAAGG AGGACTATGA GAAGTCCAGA 
GGGAAGCTCA TCGGGGCCAA GGGTGCACAG GGCGATTCTC AAATGAGCCA CTCGCTGCAA 
ATGTCCAAAC TGCAGAGCGA ATTGGAGTAC AAAAAGGGCT TCGAAGACAC CAAGTCTCAG 
TGCCACGTCT CCCTCGACAT GATCCACCTG GCGCACGCCC GCCAGGCTCA GCACTTAGCC 
ACAGACATAG GCTACAGAAC AGTGTCCCAT CACTTCACAG CGTTGCCCAC GGACATGAAG 
GTGGAGTGGG CCAAGAAGGC GTATGGCTTG CAGAGTGATA ACCAATACAG GGCAGATGTG 
AAGTGGATGA AAGGCACGGG CTGGGTCGCC ACCGGGTCAT TAAATGTGGA GCAGGCAAAG 
AAGGCAGGAG AACTCATTAG CGAGAAGAAG TACCGTCAGC ATCCAGATGC TTTGAAGTTT 
ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG 
GATAGGCTGT ACAGGGAACA AGGAGAGAAC ATAAAGCATC ATTACACGCA GACCACAGAC 
CTGCCTGAGG TCCTGCTGGC CAAGCTGAAT GCCATGAACA TCAGCGAGAC ACGGTATAAG 
GAATCCTGGA GTAAACTTCG AGACGGTGGA TATAAACTAA GGTTGGATGC CATTCCGTTC 
CAGGCAGCAA AGGCTTCTAG TGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG 
AAAATGAAAG GACAGATGCT TGGCTCCCGG AGCTTGGAAG ATGATCTCAG CCTTGCACAT 
TCAGTGCACG CGACCTCACT GCAGAGTGAT GTGAATTACA AGAAAGGCTT TGAACACTCA 
AAGGCACACT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCACGCCAA GAAGGCTCAG 
ACCCTGGCCA GCGACCAGGA CTACAGACAT CCGCTCCCCC AGCACACGTC CTTGGCAGAA 
GACCTGAGGC TGCGCTGTGC CAAGAAAGCT CACAAGTTGC AGAGCGAGAA TTTGTACCGC 
TCAGACCTGA ACTTCATGCG AGGCGTTGCC TGCGTCGTTC CAGGCACGTT GGAGATTGAA 
GGAAGAAAGA GGGCATCTGA GCTCATCAGT GAGAGTAAAT ACCGTCAGCA TCCTGGGTCG 
ATCAAGTACA CGACTGTGAC GGACACCCCC AACCTCCTTC ATGCAAAGTT CAGCAACCAG 
ATTACCAATG AGCGTCTCTA TAAAGCAGCT GGAGAGGATG CTAGACACCA GTACACAATG 
ACCCTGGGTC TGCCCGAGTT TGTCCGAGCA AAAACAAATG CGGCCAATCT GAGCGACGCA 
AAATACAAGG AGTCTTGGCA TGATCTCCGT GCTCAAGGCT ACAAGCTGAC AATAGATGCG 
CTCCCCTTCC AGGCTGCAAG GGCCTCTGGG GACATCGCCA GCGATTTTCT CTACAGACAC 
GACTTTGTGA AGGAGCGAGG GAAGCTCATC GGGATCCGCA GCGTGAGCGA TGACCCCCGG 
CTGCGGCACT GTCGGCTCAT GGGCCAGCTG CAAAGTGAGC ATCAGTACAA GAGGGAGGCA 
GCCGGCAGCC AAGCCCAGTG CCACCTACCC ATGGACATGG TGCACCTGGT CCATGCCAGG 
AAGGCCCAGG CCCTGGCCAG TGACCATGAC TACAGGACTC AGTACCACGA GTTCACGGCG 
CTACCCGAAG ACTTGAAGTT GGCCTGGGCC AAGAAAGCCC ACGCCCTGCA GAGTGAGTTT 
CGCTACAAGT CGGACCTCAT GGCCATGAAG GGGACAGGAT GGCTAGCACT GAGATCCCCA 
CAGATAGAGA GCGCCAGGAA GGCGGGAGAA CTCATCAGCG AGACCAAATA CCGTAAAAAA 
CCAGACAGTA TCAAGTTCAC CACAGTGGTT GACTCCCCAG ATCTGGTTCA CGCCAAGAAT 
AGCTACGTGC ATTGCAACGA GCGCCTGTAC AGGTTGGGGG ATGCAGAGGC CCTGCACAGA 
TACACCCTGA TCCCCGACCA CCCTGAGTTC ACCCGAGCCC GCCTCAACGC GCTGCACCTG 
AGTGACAAAG TCTACAGAAA CTCCTGGGAG CAGACCCGCG CTGGCGGCTA CGACTTCAGG 
CTGGACGCCA TCCCGTTCCA GACTGCCCGG GCATCCAGGG AGATCGCCAG CGACTTCCGG 
TATAAAGAGG CTTTCCTGCG GGACCGGGGC CTGCAGATCG GCTTCCGCAG CATCAACGAT 
GACCCGAGGA TGAAGCATTT CCTGAACGTC GGCAGGCTCC AGAGTGACAA TGAGTACAAG 
AAGGACTTTG CCAAGAGTCG GTCCCAGTTC CACAGCCGCA CAGACCAGCC TGGCTTCCTC 
CAGGCCAAGA GGAGCCAGCA GTTGGCCAGC GACGTGCATT ACCGGCAGCC CCTGCCCCAG 
CCCACGTGCG ACCCCGAGCA GCTGGGCCTC AAGCACGCGC AGAAGGCCCA CCAGCTGCAG 
AGTGACGTCA AGTACAAATC AGACTTGAAC CTGACCAGGG GTGTCGGCTG GACCCCTCCC 
GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAC TGGCCAACAT GAGGGGCCTG 
GGGCTCCCGG GGGCTTATGG GGAGCCGGAG GTGGTGGAGG CTGGAGGTCA CCCGAGGGGG 
GAGGTGAACC CAGATGCCAC TGAGATTCTG CACGTCAAAA GGAAAAAGGC TCTGTGGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005320743.1
CDS	204..5366
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_005320686.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005320686.2

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTGGAATATA AGAAGGATCT GGAAAGTAGT AAAGGTCACA GTATCAACTA CTGTGAAACG 
CCTCAGTTCA GGAACGTGAG CAAGATCTCA AAATTCACCA GTGATAATAA ATATAAAGAA 
AACTACCAGA ACCACATGAG AGGCCACTAT GAAGGAGTTG GCATGGACAG ACGCACACTT 
CATGCTCTGA AAGTTGGCAG TCTGGCCAGC AATGTTGCCT ACAAGGCTGA TTACAAACAC 
GATATTGTGG ACTACAACTA TCCAGCCACC CTTACTCCTG CCTATCAAAC AACAATGAAA 
CTGGTTCCTT TGAAAGATGT CAACTACAGG CAAAGCATCG ACAAGCTGAA GTACAGCTCT 
GTGACCAATA CTCCGCAGAT TGTTCAGGCC AAAATCAATG CCCAGCAACT GAGCCACGTG 
AACTACCGCG CTGACTATGA GAAAAACAAG TTGAATTACA CACTGCCGCA GGATGTGCCT 
CAGCTGGTGA AGGCCAGAAC TAACGCTGAA CTCTTCAGTG AGGTTAAGTA CAAAGAAGGC 
TGGGAGAAGA CAAAGGGGAA AGGATTTGAG ATGAAGCTGG ATGCCATGTC TCTGTTGGCC 
GCCAAAGCCT CGGGGGAGCT TGCTAGCAAT ATTAAATACA AAGAAGAATA TGAGAAAACA 
AAAGGCAAAG CCATGGGAAC AACCGACTCG AAGCTTCTGC ACTCCCTGCA GGTGGCCAAG 
ATGAGCAGCG AGGTTGAATA CAAGAAAGGC TTTGAGGAGA GTAAGACCCA CTTTCACCTG 
CCCATGGATA TGGTAAACAT CAGGCATGCT AAGAAGGCCC AAGCTCTTGC CAGTGACCTG 
GATTACAGGA AGAAACTGCA CGAATACACC GTGCTGCCTG AAGACATGAA GACTCAGTGG 
GCCAAGAAAG CCTACGGGCT CCAGAGCGAG CTGCAGTACA AATCCGACCT GGCGTGGATG 
AAGGGAGTCG GGTGGCTGAC TGAGGGCAGT CTCAATTTGG AACAGGCCAA GAAGGCTGGA 
CAACTGGTCA GCGAGAAAAA CTACCGGCAG AGGGTTGATG AGCTGAAGTT CACCAGTGTG 
ACCGATAGTG CGCAGATGGA GCACGCCAAG AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC 
TACAAGGCAG AGAACCAGCA GTCTATCCAC CAGTACACCA TCAGCAAAGA CGAGCCTCTC 
TTCCTGCAGG CCCGGGCCAA TGCTGCTCAC CTCAGCGAGA AACGGTACAA GAGCAGCTGG 
GAAAACCAGA AGGCAAGAGG GTTTGAGCTG AGTCTTGATT CCTTGACCTT CCTGGCAGCC 
AAAGCCAAGA GGGACCTGGC GAGCGAGGTG AAGTACAAGG AGGACTATGA GAAGTCCAGA 
GGGAAGCTCA TCGGGGCCAA GGGTGCACAG GGCGATTCTC AAATGAGCCA CTCGCTGCAA 
ATGTCCAAAC TGCAGAGCGA ATTGGAGTAC AAAAAGGGCT TCGAAGACAC CAAGTCTCAG 
TGCCACGTCT CCCTCGACAT GATCCACCTG GCGCACGCCC GCCAGGCTCA GCACTTAGCC 
ACAGACATAG GCTACAGAAC AGTGTCCCAT CACTTCACAG CGTTGCCCAC GGACATGAAG 
GTGGAGTGGG CCAAGAAGGC GTATGGCTTG CAGAGTGATA ACCAATACAG GGCAGATGTG 
AAGTGGATGA AAGGCACGGG CTGGGTCGCC ACCGGGTCAT TAAATGTGGA GCAGGCAAAG 
AAGGCAGGAG AACTCATTAG CGAGAAGAAG TACCGTCAGC ATCCAGATGC TTTGAAGTTT 
ACCAGTATTA AAGACACTCC GGAGATGGTC CAGGCCAGAA TTAGTTATAC CCAAGCAGTG 
GATAGGCTGT ACAGGGAACA AGGAGAGAAC ATAAAGCATC ATTACACGCA GACCACAGAC 
CTGCCTGAGG TCCTGCTGGC CAAGCTGAAT GCCATGAACA TCAGCGAGAC ACGGTATAAG 
GAATCCTGGA GTAAACTTCG AGACGGTGGA TATAAACTAA GGTTGGATGC CATTCCGTTC 
CAGGCAGCAA AGGCTTCTAG TGAAATCATA AGTGATTACA AATACAAAGA GGCATTTGAG 
AAAATGAAAG GACAGATGCT TGGCTCCCGG AGCTTGGAAG ATGATCTCAG CCTTGCACAT 
TCAGTGCACG CGACCTCACT GCAGAGTGAT GTGAATTACA AGAAAGGCTT TGAACACTCA 
AAGGCACACT TCCATCTGCC GTTGGACATG GTAACCCTGG TGCACGCCAA GAAGGCTCAG 
ACCCTGGCCA GCGACCAGGA CTACAGACAT CCGCTCCCCC AGCACACGTC CTTGGCAGAA 
GACCTGAGGC TGCGCTGTGC CAAGAAAGCT CACAAGTTGC AGAGCGAGAA TTTGTACCGC 
TCAGACCTGA ACTTCATGCG AGGCGTTGCC TGCGTCGTTC CAGGCACGTT GGAGATTGAA 
GGAAGAAAGA GGGCATCTGA GCTCATCAGT GAGAGTAAAT ACCGTCAGCA TCCTGGGTCG 
ATCAAGTACA CGACTGTGAC GGACACCCCC AACCTCCTTC ATGCAAAGTT CAGCAACCAG 
ATTACCAATG AGCGTCTCTA TAAAGCAGCT GGAGAGGATG CTAGACACCA GTACACAATG 
ACCCTGGGTC TGCCCGAGTT TGTCCGAGCA AAAACAAATG CGGCCAATCT GAGCGACGCA 
AAATACAAGG AGTCTTGGCA TGATCTCCGT GCTCAAGGCT ACAAGCTGAC AATAGATGCG 
CTCCCCTTCC AGGCTGCAAG GGCCTCTGGG GACATCGCCA GCGATTTTCT CTACAGACAC 
GACTTTGTGA AGGAGCGAGG GAAGCTCATC GGGATCCGCA GCGTGAGCGA TGACCCCCGG 
CTGCGGCACT GTCGGCTCAT GGGCCAGCTG CAAAGTGAGC ATCAGTACAA GAGGGAGGCA 
GCCGGCAGCC AAGCCCAGTG CCACCTACCC ATGGACATGG TGCACCTGGT CCATGCCAGG 
AAGGCCCAGG CCCTGGCCAG TGACCATGAC TACAGGACTC AGTACCACGA GTTCACGGCG 
CTACCCGAAG ACTTGAAGTT GGCCTGGGCC AAGAAAGCCC ACGCCCTGCA GAGTGAGTTT 
CGCTACAAGT CGGACCTCAT GGCCATGAAG GGGACAGGAT GGCTAGCACT GAGATCCCCA 
CAGATAGAGA GCGCCAGGAA GGCGGGAGAA CTCATCAGCG AGACCAAATA CCGTAAAAAA 
CCAGACAGTA TCAAGTTCAC CACAGTGGTT GACTCCCCAG ATCTGGTTCA CGCCAAGAAT 
AGCTACGTGC ATTGCAACGA GCGCCTGTAC AGGTTGGGGG ATGCAGAGGC CCTGCACAGA 
TACACCCTGA TCCCCGACCA CCCTGAGTTC ACCCGAGCCC GCCTCAACGC GCTGCACCTG 
AGTGACAAAG TCTACAGAAA CTCCTGGGAG CAGACCCGCG CTGGCGGCTA CGACTTCAGG 
CTGGACGCCA TCCCGTTCCA GACTGCCCGG GCATCCAGGG AGATCGCCAG CGACTTCCGG 
TATAAAGAGG CTTTCCTGCG GGACCGGGGC CTGCAGATCG GCTTCCGCAG CATCAACGAT 
GACCCGAGGA TGAAGCATTT CCTGAACGTC GGCAGGCTCC AGAGTGACAA TGAGTACAAG 
AAGGACTTTG CCAAGAGTCG GTCCCAGTTC CACAGCCGCA CAGACCAGCC TGGCTTCCTC 
CAGGCCAAGA GGAGCCAGCA GTTGGCCAGC GACGTGCATT ACCGGCAGCC CCTGCCCCAG 
CCCACGTGCG ACCCCGAGCA GCTGGGCCTC AAGCACGCGC AGAAGGCCCA CCAGCTGCAG 
AGTGACGTCA AGTACAAATC AGACTTGAAC CTGACCAGGG GTGTCGGCTG GACCCCTCCC 
GGCTCCTACA AAGTGGAAAT GGCTCGGCGG GCTGCAGAAC TGGCCAACAT GAGGGGCCTG 
GGGCTCCCGG GGGCTTATGG GGAGCCGGAG GTGGTGGAGG CTGGAGGTCA CCCGAGGGGG 
GAGGTGAACC CAGATGCCAC TGAGATTCTG CACGTCAAAA GGAAAAAGGC TCTGTGGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_013356569.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013212023.1 Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc52002
Clone ID Related Accession (Same CDS sequence)	XM_013356569.1 , XM_013356569.2
Accession Version	XM_013356569.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
AATAAATATA AAGAAAACTA CCAGAACCAC ATGAGAGGCC ACTATGAAGG AGTTGGCATG 
GACAGACGCA CACTTCATGC TCTGAAAGTT GGCAGTCTGG CCAGCAATGT TGCCTACAAG 
GCTGATTACA AACACGATAT TGTGGACTAC AACTATCCAG CCACCCTTAC TCCTGCCTAT 
CAAACAACAA TGAAACTGGT TCCTTTGAAA GATGTCAACT ACAGGCAAAG CATCGACAAG 
CTGAAGTACA GCTCTGTGAC CAATACTCCG CAGATTGTTC AGGCCAAAAT CAATGCCCAG 
CAACTGAGCC ACGTGAACTA CCGCGCTGAC TATGAGAAAA ACAAGTTGAA TTACACACTG 
CCGCAGGATG TGCCTCAGCT GGTGAAGGCC AGAACTAACG CTGAACTCTT CAGTGAGGTT 
AAGTACAAAG AAGGCTGGGA GAAGACAAAG GGGAAAGGAT TTGAGATGAA GCTGGATGCC 
ATGTCTCTGT TGGCCGCCAA AGCCTCGGGG GAGCTTGCTA GCAATATTAA ATACAAAGAA 
GAATATGAGA AAACAAAAGG CAAAGCCATG GGAACAACCG ACTCGAAGCT TCTGCACTCC 
CTGCAGGTGG CCAAGATGAG CAGCGAGGTT GAATACAAGA AAGGCTTTGA GGAGAGTAAG 
ACCCACTTTC ACCTGCCCAT GGATATGGTA AACATCAGGC ATGCTAAGAA GGCCCAAGCT 
CTTGCCAGTG ACCTGGATTA CAGGAAGAAA CTGCACGAAT ACACCGTGCT GCCTGAAGAC 
ATGAAGACTC AGTGGGCCAA GAAAGCCTAC GGGCTCCAGA GCGAGCTGCA GTACAAATCC 
GACCTGGCGT GGATGAAGGG AGTCGGGTGG CTGACTGAGG GCAGTCTCAA TTTGGAACAG 
GCCAAGAAGG CTGGACAACT GGTCAGCGAG AAAAACTACC GGCAGAGGGT TGATGAGCTG 
AAGTTCACCA GTGTGACCGA TAGTGCGCAG ATGGAGCACG CCAAGAAGAG CCAGGAGCTG 
CAGAGCGGGG TGGCCTACAA GGCAGAGAAC CAGCAGTCTA TCCACCAGTA CACCATCAGC 
AAAGACGAGC CTCTCTTCCT GCAGGCCCGG GCCAATGCTG CTCACCTCAG CGAGAAACGG 
TACAAGAGCA GCTGGGAAAA CCAGAAGGCA AGAGGGTTTG AGCTGAGTCT TGATTCCTTG 
ACCTTCCTGG CAGCCAAAGC CAAGAGGGAC CTGGCGAGCG AGGTGAAGTA CAAGGAGGAC 
TATGAGAAGT CCAGAGGGAA GCTCATCGGG GCCAAGGGTG CACAGGGCGA TTCTCAAATG 
AGCCACTCGC TGCAAATGTC CAAACTGCAG AGCGAATTGG AGTACAAAAA GGGCTTCGAA 
GACACCAAGT CTCAGTGCCA CGTCTCCCTC GACATGATCC ACCTGGCGCA CGCCCGCCAG 
GCTCAGCACT TAGCCACAGA CATAGGCTAC AGAACAGTGT CCCATCACTT CACAGCGTTG 
CCCACGGACA TGAAGGTGGA GTGGGCCAAG AAGGCGTATG GCTTGCAGAG TGATAACCAA 
TACAGGGCAG ATGTGAAGTG GATGAAAGGC ACGGGCTGGG TCGCCACCGG GTCATTAAAT 
GTGGAGCAGG CAAAGAAGGC AGGAGAACTC ATTAGCGAGA AGAAGTACCG TCAGCATCCA 
GATGCTTTGA AGTTTACCAG TATTAAAGAC ACTCCGGAGA TGGTCCAGGC CAGAATTAGT 
TATACCCAAG CAGTGGATAG GCTGTACAGG GAACAAGGAG AGAACATAAA GCATCATTAC 
ACGCAGACCA CAGACCTGCC TGAGGTCCTG CTGGCCAAGC TGAATGCCAT GAACATCAGC 
GAGACACGGT ATAAGGAATC CTGGAGTAAA CTTCGAGACG GTGGATATAA ACTAAGGTTG 
GATGCCATTC CGTTCCAGGC AGCAAAGGCT TCTAGTGAAA TCATAAGTGA TTACAAATAC 
AAAGAGGCAT TTGAGAAAAT GAAAGGACAG ATGCTTGGCT CCCGGAGCTT GGAAGATGAT 
CTCAGCCTTG CACATTCAGT GCACGCGACC TCACTGCAGA GTGATGTGAA TTACAAGAAA 
GGCTTTGAAC ACTCAAAGGC ACACTTCCAT CTGCCGTTGG ACATGGTAAC CCTGGTGCAC 
GCCAAGAAGG CTCAGACCCT GGCCAGCGAC CAGGACTACA GACATCCGCT CCCCCAGCAC 
ACGTCCTTGG CAGAAGACCT GAGGCTGCGC TGTGCCAAGA AAGCTCACAA GTTGCAGAGC 
GAGAATTTGT ACCGCTCAGA CCTGAACTTC ATGCGAGGCG TTGCCTGCGT CGTTCCAGGC 
ACGTTGGAGA TTGAAGGAAG AAAGAGGGCA TCTGAGCTCA TCAGTGAGAG TAAATACCGT 
CAGCATCCTG GGTCGATCAA GTACACGACT GTGACGGACA CCCCCAACCT CCTTCATGCA 
AAGTTCAGCA ACCAGATTAC CAATGAGCGT CTCTATAAAG CAGCTGGAGA GGATGCTAGA 
CACCAGTACA CAATGACCCT GGGTCTGCCC GAGTTTGTCC GAGCAAAAAC AAATGCGGCC 
AATCTGAGCG ACGCAAAATA CAAGGAGTCT TGGCATGATC TCCGTGCTCA AGGCTACAAG 
CTGACAATAG ATGCGCTCCC CTTCCAGGCT GCAAGGGCCT CTGGGGACAT CGCCAGCGAT 
TTTCTCTACA GACACGACTT TGTGAAGGAG CGAGGGAAGC TCATCGGGAT CCGCAGCGTG 
AGCGATGACC CCCGGCTGCG GCACTGTCGG CTCATGGGCC AGCTGCAAAG TGAGCATCAG 
TACAAGAGGG AGGCAGCCGG CAGCCAAGCC CAGTGCCACC TACCCATGGA CATGGTGCAC 
CTGGTCCATG CCAGGAAGGC CCAGGCCCTG GCCAGTGACC ATGACTACAG GACTCAGTAC 
CACGAGTTCA CGGCGCTACC CGAAGACTTG AAGTTGGCCT GGGCCAAGAA AGCCCACGCC 
CTGCAGAGTG AGTTTCGCTA CAAGTCGGAC CTCATGGCCA TGAAGGGGAC AGGATGGCTA 
GCACTGAGAT CCCCACAGAT AGAGAGCGCC AGGAAGGCGG GAGAACTCAT CAGCGAGACC 
AAATACCGTA AAAAACCAGA CAGTATCAAG TTCACCACAG TGGTTGACTC CCCAGATCTG 
GTTCACGCCA AGAATAGCTA CGTGCATTGC AACGAGCGCC TGTACAGGTT GGGGGATGCA 
GAGGCCCTGC ACAGATACAC CCTGATCCCC GACCACCCTG AGTTCACCCG AGCCCGCCTC 
AACGCGCTGC ACCTGAGTGA CAAAGTCTAC AGAAACTCCT GGGAGCAGAC CCGCGCTGGC 
GGCTACGACT TCAGGCTGGA CGCCATCCCG TTCCAGACTG CCCGGGCATC CAGGGAGATC 
GCCAGCGACT TCCGGTATAA AGAGGCTTTC CTGCGGGACC GGGGCCTGCA GATCGGCTTC 
CGCAGCATCA ACGATGACCC GAGGATGAAG CATTTCCTGA ACGTCGGCAG GCTCCAGAGT 
GACAATGAGT ACAAGAAGGA CTTTGCCAAG AGTCGGTCCC AGTTCCACAG CCGCACAGAC 
CAGCCTGGCT TCCTCCAGGC CAAGAGGAGC CAGCAGTTGG CCAGCGACGT GCATTACCGG 
CAGCCCCTGC CCCAGCCCAC GTGCGACCCC GAGCAGCTGG GCCTCAAGCA CGCGCAGAAG 
GCCCACCAGC TGCAGAGTGA CGTCAAGTAC AAATCAGACT TGAACCTGAC CAGGGGTGTC 
GGCTGGACCC CTCCCGGCTC CTACAAAGTG GAAATGGCTC GGCGGGCTGC AGAACTGGCC 
AACATGAGGG GCCTGGGGCT CCCGGGGGCT TATGGGGAGC CGGAGGTGGT GGAGGCTGGA 
GGTCACCCGA GGGGGGAGGT GAACCCAGAT GCCACTGAGA TTCTGCACGT CAAAAGGAAA 
AAGGCTCTGT GGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013212023.1
CDS	204..5261
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_013356569.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356569.1

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
AATAAATATA AAGAAAACTA CCAGAACCAC ATGAGAGGCC ACTATGAAGG AGTTGGCATG 
GACAGACGCA CACTTCATGC TCTGAAAGTT GGCAGTCTGG CCAGCAATGT TGCCTACAAG 
GCTGATTACA AACACGATAT TGTGGACTAC AACTATCCAG CCACCCTTAC TCCTGCCTAT 
CAAACAACAA TGAAACTGGT TCCTTTGAAA GATGTCAACT ACAGGCAAAG CATCGACAAG 
CTGAAGTACA GCTCTGTGAC CAATACTCCG CAGATTGTTC AGGCCAAAAT CAATGCCCAG 
CAACTGAGCC ACGTGAACTA CCGCGCTGAC TATGAGAAAA ACAAGTTGAA TTACACACTG 
CCGCAGGATG TGCCTCAGCT GGTGAAGGCC AGAACTAACG CTGAACTCTT CAGTGAGGTT 
AAGTACAAAG AAGGCTGGGA GAAGACAAAG GGGAAAGGAT TTGAGATGAA GCTGGATGCC 
ATGTCTCTGT TGGCCGCCAA AGCCTCGGGG GAGCTTGCTA GCAATATTAA ATACAAAGAA 
GAATATGAGA AAACAAAAGG CAAAGCCATG GGAACAACCG ACTCGAAGCT TCTGCACTCC 
CTGCAGGTGG CCAAGATGAG CAGCGAGGTT GAATACAAGA AAGGCTTTGA GGAGAGTAAG 
ACCCACTTTC ACCTGCCCAT GGATATGGTA AACATCAGGC ATGCTAAGAA GGCCCAAGCT 
CTTGCCAGTG ACCTGGATTA CAGGAAGAAA CTGCACGAAT ACACCGTGCT GCCTGAAGAC 
ATGAAGACTC AGTGGGCCAA GAAAGCCTAC GGGCTCCAGA GCGAGCTGCA GTACAAATCC 
GACCTGGCGT GGATGAAGGG AGTCGGGTGG CTGACTGAGG GCAGTCTCAA TTTGGAACAG 
GCCAAGAAGG CTGGACAACT GGTCAGCGAG AAAAACTACC GGCAGAGGGT TGATGAGCTG 
AAGTTCACCA GTGTGACCGA TAGTGCGCAG ATGGAGCACG CCAAGAAGAG CCAGGAGCTG 
CAGAGCGGGG TGGCCTACAA GGCAGAGAAC CAGCAGTCTA TCCACCAGTA CACCATCAGC 
AAAGACGAGC CTCTCTTCCT GCAGGCCCGG GCCAATGCTG CTCACCTCAG CGAGAAACGG 
TACAAGAGCA GCTGGGAAAA CCAGAAGGCA AGAGGGTTTG AGCTGAGTCT TGATTCCTTG 
ACCTTCCTGG CAGCCAAAGC CAAGAGGGAC CTGGCGAGCG AGGTGAAGTA CAAGGAGGAC 
TATGAGAAGT CCAGAGGGAA GCTCATCGGG GCCAAGGGTG CACAGGGCGA TTCTCAAATG 
AGCCACTCGC TGCAAATGTC CAAACTGCAG AGCGAATTGG AGTACAAAAA GGGCTTCGAA 
GACACCAAGT CTCAGTGCCA CGTCTCCCTC GACATGATCC ACCTGGCGCA CGCCCGCCAG 
GCTCAGCACT TAGCCACAGA CATAGGCTAC AGAACAGTGT CCCATCACTT CACAGCGTTG 
CCCACGGACA TGAAGGTGGA GTGGGCCAAG AAGGCGTATG GCTTGCAGAG TGATAACCAA 
TACAGGGCAG ATGTGAAGTG GATGAAAGGC ACGGGCTGGG TCGCCACCGG GTCATTAAAT 
GTGGAGCAGG CAAAGAAGGC AGGAGAACTC ATTAGCGAGA AGAAGTACCG TCAGCATCCA 
GATGCTTTGA AGTTTACCAG TATTAAAGAC ACTCCGGAGA TGGTCCAGGC CAGAATTAGT 
TATACCCAAG CAGTGGATAG GCTGTACAGG GAACAAGGAG AGAACATAAA GCATCATTAC 
ACGCAGACCA CAGACCTGCC TGAGGTCCTG CTGGCCAAGC TGAATGCCAT GAACATCAGC 
GAGACACGGT ATAAGGAATC CTGGAGTAAA CTTCGAGACG GTGGATATAA ACTAAGGTTG 
GATGCCATTC CGTTCCAGGC AGCAAAGGCT TCTAGTGAAA TCATAAGTGA TTACAAATAC 
AAAGAGGCAT TTGAGAAAAT GAAAGGACAG ATGCTTGGCT CCCGGAGCTT GGAAGATGAT 
CTCAGCCTTG CACATTCAGT GCACGCGACC TCACTGCAGA GTGATGTGAA TTACAAGAAA 
GGCTTTGAAC ACTCAAAGGC ACACTTCCAT CTGCCGTTGG ACATGGTAAC CCTGGTGCAC 
GCCAAGAAGG CTCAGACCCT GGCCAGCGAC CAGGACTACA GACATCCGCT CCCCCAGCAC 
ACGTCCTTGG CAGAAGACCT GAGGCTGCGC TGTGCCAAGA AAGCTCACAA GTTGCAGAGC 
GAGAATTTGT ACCGCTCAGA CCTGAACTTC ATGCGAGGCG TTGCCTGCGT CGTTCCAGGC 
ACGTTGGAGA TTGAAGGAAG AAAGAGGGCA TCTGAGCTCA TCAGTGAGAG TAAATACCGT 
CAGCATCCTG GGTCGATCAA GTACACGACT GTGACGGACA CCCCCAACCT CCTTCATGCA 
AAGTTCAGCA ACCAGATTAC CAATGAGCGT CTCTATAAAG CAGCTGGAGA GGATGCTAGA 
CACCAGTACA CAATGACCCT GGGTCTGCCC GAGTTTGTCC GAGCAAAAAC AAATGCGGCC 
AATCTGAGCG ACGCAAAATA CAAGGAGTCT TGGCATGATC TCCGTGCTCA AGGCTACAAG 
CTGACAATAG ATGCGCTCCC CTTCCAGGCT GCAAGGGCCT CTGGGGACAT CGCCAGCGAT 
TTTCTCTACA GACACGACTT TGTGAAGGAG CGAGGGAAGC TCATCGGGAT CCGCAGCGTG 
AGCGATGACC CCCGGCTGCG GCACTGTCGG CTCATGGGCC AGCTGCAAAG TGAGCATCAG 
TACAAGAGGG AGGCAGCCGG CAGCCAAGCC CAGTGCCACC TACCCATGGA CATGGTGCAC 
CTGGTCCATG CCAGGAAGGC CCAGGCCCTG GCCAGTGACC ATGACTACAG GACTCAGTAC 
CACGAGTTCA CGGCGCTACC CGAAGACTTG AAGTTGGCCT GGGCCAAGAA AGCCCACGCC 
CTGCAGAGTG AGTTTCGCTA CAAGTCGGAC CTCATGGCCA TGAAGGGGAC AGGATGGCTA 
GCACTGAGAT CCCCACAGAT AGAGAGCGCC AGGAAGGCGG GAGAACTCAT CAGCGAGACC 
AAATACCGTA AAAAACCAGA CAGTATCAAG TTCACCACAG TGGTTGACTC CCCAGATCTG 
GTTCACGCCA AGAATAGCTA CGTGCATTGC AACGAGCGCC TGTACAGGTT GGGGGATGCA 
GAGGCCCTGC ACAGATACAC CCTGATCCCC GACCACCCTG AGTTCACCCG AGCCCGCCTC 
AACGCGCTGC ACCTGAGTGA CAAAGTCTAC AGAAACTCCT GGGAGCAGAC CCGCGCTGGC 
GGCTACGACT TCAGGCTGGA CGCCATCCCG TTCCAGACTG CCCGGGCATC CAGGGAGATC 
GCCAGCGACT TCCGGTATAA AGAGGCTTTC CTGCGGGACC GGGGCCTGCA GATCGGCTTC 
CGCAGCATCA ACGATGACCC GAGGATGAAG CATTTCCTGA ACGTCGGCAG GCTCCAGAGT 
GACAATGAGT ACAAGAAGGA CTTTGCCAAG AGTCGGTCCC AGTTCCACAG CCGCACAGAC 
CAGCCTGGCT TCCTCCAGGC CAAGAGGAGC CAGCAGTTGG CCAGCGACGT GCATTACCGG 
CAGCCCCTGC CCCAGCCCAC GTGCGACCCC GAGCAGCTGG GCCTCAAGCA CGCGCAGAAG 
GCCCACCAGC TGCAGAGTGA CGTCAAGTAC AAATCAGACT TGAACCTGAC CAGGGGTGTC 
GGCTGGACCC CTCCCGGCTC CTACAAAGTG GAAATGGCTC GGCGGGCTGC AGAACTGGCC 
AACATGAGGG GCCTGGGGCT CCCGGGGGCT TATGGGGAGC CGGAGGTGGT GGAGGCTGGA 
GGTCACCCGA GGGGGGAGGT GAACCCAGAT GCCACTGAGA TTCTGCACGT CAAAAGGAAA 
AAGGCTCTGT GGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_013356569.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013212023.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X3, mRNA.

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CloneID	OIc52002
Clone ID Related Accession (Same CDS sequence)	XM_013356569.1 , XM_013356569.2
Accession Version	XM_013356569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013356569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
AATAAATATA AAGAAAACTA CCAGAACCAC ATGAGAGGCC ACTATGAAGG AGTTGGCATG 
GACAGACGCA CACTTCATGC TCTGAAAGTT GGCAGTCTGG CCAGCAATGT TGCCTACAAG 
GCTGATTACA AACACGATAT TGTGGACTAC AACTATCCAG CCACCCTTAC TCCTGCCTAT 
CAAACAACAA TGAAACTGGT TCCTTTGAAA GATGTCAACT ACAGGCAAAG CATCGACAAG 
CTGAAGTACA GCTCTGTGAC CAATACTCCG CAGATTGTTC AGGCCAAAAT CAATGCCCAG 
CAACTGAGCC ACGTGAACTA CCGCGCTGAC TATGAGAAAA ACAAGTTGAA TTACACACTG 
CCGCAGGATG TGCCTCAGCT GGTGAAGGCC AGAACTAACG CTGAACTCTT CAGTGAGGTT 
AAGTACAAAG AAGGCTGGGA GAAGACAAAG GGGAAAGGAT TTGAGATGAA GCTGGATGCC 
ATGTCTCTGT TGGCCGCCAA AGCCTCGGGG GAGCTTGCTA GCAATATTAA ATACAAAGAA 
GAATATGAGA AAACAAAAGG CAAAGCCATG GGAACAACCG ACTCGAAGCT TCTGCACTCC 
CTGCAGGTGG CCAAGATGAG CAGCGAGGTT GAATACAAGA AAGGCTTTGA GGAGAGTAAG 
ACCCACTTTC ACCTGCCCAT GGATATGGTA AACATCAGGC ATGCTAAGAA GGCCCAAGCT 
CTTGCCAGTG ACCTGGATTA CAGGAAGAAA CTGCACGAAT ACACCGTGCT GCCTGAAGAC 
ATGAAGACTC AGTGGGCCAA GAAAGCCTAC GGGCTCCAGA GCGAGCTGCA GTACAAATCC 
GACCTGGCGT GGATGAAGGG AGTCGGGTGG CTGACTGAGG GCAGTCTCAA TTTGGAACAG 
GCCAAGAAGG CTGGACAACT GGTCAGCGAG AAAAACTACC GGCAGAGGGT TGATGAGCTG 
AAGTTCACCA GTGTGACCGA TAGTGCGCAG ATGGAGCACG CCAAGAAGAG CCAGGAGCTG 
CAGAGCGGGG TGGCCTACAA GGCAGAGAAC CAGCAGTCTA TCCACCAGTA CACCATCAGC 
AAAGACGAGC CTCTCTTCCT GCAGGCCCGG GCCAATGCTG CTCACCTCAG CGAGAAACGG 
TACAAGAGCA GCTGGGAAAA CCAGAAGGCA AGAGGGTTTG AGCTGAGTCT TGATTCCTTG 
ACCTTCCTGG CAGCCAAAGC CAAGAGGGAC CTGGCGAGCG AGGTGAAGTA CAAGGAGGAC 
TATGAGAAGT CCAGAGGGAA GCTCATCGGG GCCAAGGGTG CACAGGGCGA TTCTCAAATG 
AGCCACTCGC TGCAAATGTC CAAACTGCAG AGCGAATTGG AGTACAAAAA GGGCTTCGAA 
GACACCAAGT CTCAGTGCCA CGTCTCCCTC GACATGATCC ACCTGGCGCA CGCCCGCCAG 
GCTCAGCACT TAGCCACAGA CATAGGCTAC AGAACAGTGT CCCATCACTT CACAGCGTTG 
CCCACGGACA TGAAGGTGGA GTGGGCCAAG AAGGCGTATG GCTTGCAGAG TGATAACCAA 
TACAGGGCAG ATGTGAAGTG GATGAAAGGC ACGGGCTGGG TCGCCACCGG GTCATTAAAT 
GTGGAGCAGG CAAAGAAGGC AGGAGAACTC ATTAGCGAGA AGAAGTACCG TCAGCATCCA 
GATGCTTTGA AGTTTACCAG TATTAAAGAC ACTCCGGAGA TGGTCCAGGC CAGAATTAGT 
TATACCCAAG CAGTGGATAG GCTGTACAGG GAACAAGGAG AGAACATAAA GCATCATTAC 
ACGCAGACCA CAGACCTGCC TGAGGTCCTG CTGGCCAAGC TGAATGCCAT GAACATCAGC 
GAGACACGGT ATAAGGAATC CTGGAGTAAA CTTCGAGACG GTGGATATAA ACTAAGGTTG 
GATGCCATTC CGTTCCAGGC AGCAAAGGCT TCTAGTGAAA TCATAAGTGA TTACAAATAC 
AAAGAGGCAT TTGAGAAAAT GAAAGGACAG ATGCTTGGCT CCCGGAGCTT GGAAGATGAT 
CTCAGCCTTG CACATTCAGT GCACGCGACC TCACTGCAGA GTGATGTGAA TTACAAGAAA 
GGCTTTGAAC ACTCAAAGGC ACACTTCCAT CTGCCGTTGG ACATGGTAAC CCTGGTGCAC 
GCCAAGAAGG CTCAGACCCT GGCCAGCGAC CAGGACTACA GACATCCGCT CCCCCAGCAC 
ACGTCCTTGG CAGAAGACCT GAGGCTGCGC TGTGCCAAGA AAGCTCACAA GTTGCAGAGC 
GAGAATTTGT ACCGCTCAGA CCTGAACTTC ATGCGAGGCG TTGCCTGCGT CGTTCCAGGC 
ACGTTGGAGA TTGAAGGAAG AAAGAGGGCA TCTGAGCTCA TCAGTGAGAG TAAATACCGT 
CAGCATCCTG GGTCGATCAA GTACACGACT GTGACGGACA CCCCCAACCT CCTTCATGCA 
AAGTTCAGCA ACCAGATTAC CAATGAGCGT CTCTATAAAG CAGCTGGAGA GGATGCTAGA 
CACCAGTACA CAATGACCCT GGGTCTGCCC GAGTTTGTCC GAGCAAAAAC AAATGCGGCC 
AATCTGAGCG ACGCAAAATA CAAGGAGTCT TGGCATGATC TCCGTGCTCA AGGCTACAAG 
CTGACAATAG ATGCGCTCCC CTTCCAGGCT GCAAGGGCCT CTGGGGACAT CGCCAGCGAT 
TTTCTCTACA GACACGACTT TGTGAAGGAG CGAGGGAAGC TCATCGGGAT CCGCAGCGTG 
AGCGATGACC CCCGGCTGCG GCACTGTCGG CTCATGGGCC AGCTGCAAAG TGAGCATCAG 
TACAAGAGGG AGGCAGCCGG CAGCCAAGCC CAGTGCCACC TACCCATGGA CATGGTGCAC 
CTGGTCCATG CCAGGAAGGC CCAGGCCCTG GCCAGTGACC ATGACTACAG GACTCAGTAC 
CACGAGTTCA CGGCGCTACC CGAAGACTTG AAGTTGGCCT GGGCCAAGAA AGCCCACGCC 
CTGCAGAGTG AGTTTCGCTA CAAGTCGGAC CTCATGGCCA TGAAGGGGAC AGGATGGCTA 
GCACTGAGAT CCCCACAGAT AGAGAGCGCC AGGAAGGCGG GAGAACTCAT CAGCGAGACC 
AAATACCGTA AAAAACCAGA CAGTATCAAG TTCACCACAG TGGTTGACTC CCCAGATCTG 
GTTCACGCCA AGAATAGCTA CGTGCATTGC AACGAGCGCC TGTACAGGTT GGGGGATGCA 
GAGGCCCTGC ACAGATACAC CCTGATCCCC GACCACCCTG AGTTCACCCG AGCCCGCCTC 
AACGCGCTGC ACCTGAGTGA CAAAGTCTAC AGAAACTCCT GGGAGCAGAC CCGCGCTGGC 
GGCTACGACT TCAGGCTGGA CGCCATCCCG TTCCAGACTG CCCGGGCATC CAGGGAGATC 
GCCAGCGACT TCCGGTATAA AGAGGCTTTC CTGCGGGACC GGGGCCTGCA GATCGGCTTC 
CGCAGCATCA ACGATGACCC GAGGATGAAG CATTTCCTGA ACGTCGGCAG GCTCCAGAGT 
GACAATGAGT ACAAGAAGGA CTTTGCCAAG AGTCGGTCCC AGTTCCACAG CCGCACAGAC 
CAGCCTGGCT TCCTCCAGGC CAAGAGGAGC CAGCAGTTGG CCAGCGACGT GCATTACCGG 
CAGCCCCTGC CCCAGCCCAC GTGCGACCCC GAGCAGCTGG GCCTCAAGCA CGCGCAGAAG 
GCCCACCAGC TGCAGAGTGA CGTCAAGTAC AAATCAGACT TGAACCTGAC CAGGGGTGTC 
GGCTGGACCC CTCCCGGCTC CTACAAAGTG GAAATGGCTC GGCGGGCTGC AGAACTGGCC 
AACATGAGGG GCCTGGGGCT CCCGGGGGCT TATGGGGAGC CGGAGGTGGT GGAGGCTGGA 
GGTCACCCGA GGGGGGAGGT GAACCCAGAT GCCACTGAGA TTCTGCACGT CAAAAGGAAA 
AAGGCTCTGT GGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013212023.1
CDS	237..5294
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_013356569.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356569.2

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
AATAAATATA AAGAAAACTA CCAGAACCAC ATGAGAGGCC ACTATGAAGG AGTTGGCATG 
GACAGACGCA CACTTCATGC TCTGAAAGTT GGCAGTCTGG CCAGCAATGT TGCCTACAAG 
GCTGATTACA AACACGATAT TGTGGACTAC AACTATCCAG CCACCCTTAC TCCTGCCTAT 
CAAACAACAA TGAAACTGGT TCCTTTGAAA GATGTCAACT ACAGGCAAAG CATCGACAAG 
CTGAAGTACA GCTCTGTGAC CAATACTCCG CAGATTGTTC AGGCCAAAAT CAATGCCCAG 
CAACTGAGCC ACGTGAACTA CCGCGCTGAC TATGAGAAAA ACAAGTTGAA TTACACACTG 
CCGCAGGATG TGCCTCAGCT GGTGAAGGCC AGAACTAACG CTGAACTCTT CAGTGAGGTT 
AAGTACAAAG AAGGCTGGGA GAAGACAAAG GGGAAAGGAT TTGAGATGAA GCTGGATGCC 
ATGTCTCTGT TGGCCGCCAA AGCCTCGGGG GAGCTTGCTA GCAATATTAA ATACAAAGAA 
GAATATGAGA AAACAAAAGG CAAAGCCATG GGAACAACCG ACTCGAAGCT TCTGCACTCC 
CTGCAGGTGG CCAAGATGAG CAGCGAGGTT GAATACAAGA AAGGCTTTGA GGAGAGTAAG 
ACCCACTTTC ACCTGCCCAT GGATATGGTA AACATCAGGC ATGCTAAGAA GGCCCAAGCT 
CTTGCCAGTG ACCTGGATTA CAGGAAGAAA CTGCACGAAT ACACCGTGCT GCCTGAAGAC 
ATGAAGACTC AGTGGGCCAA GAAAGCCTAC GGGCTCCAGA GCGAGCTGCA GTACAAATCC 
GACCTGGCGT GGATGAAGGG AGTCGGGTGG CTGACTGAGG GCAGTCTCAA TTTGGAACAG 
GCCAAGAAGG CTGGACAACT GGTCAGCGAG AAAAACTACC GGCAGAGGGT TGATGAGCTG 
AAGTTCACCA GTGTGACCGA TAGTGCGCAG ATGGAGCACG CCAAGAAGAG CCAGGAGCTG 
CAGAGCGGGG TGGCCTACAA GGCAGAGAAC CAGCAGTCTA TCCACCAGTA CACCATCAGC 
AAAGACGAGC CTCTCTTCCT GCAGGCCCGG GCCAATGCTG CTCACCTCAG CGAGAAACGG 
TACAAGAGCA GCTGGGAAAA CCAGAAGGCA AGAGGGTTTG AGCTGAGTCT TGATTCCTTG 
ACCTTCCTGG CAGCCAAAGC CAAGAGGGAC CTGGCGAGCG AGGTGAAGTA CAAGGAGGAC 
TATGAGAAGT CCAGAGGGAA GCTCATCGGG GCCAAGGGTG CACAGGGCGA TTCTCAAATG 
AGCCACTCGC TGCAAATGTC CAAACTGCAG AGCGAATTGG AGTACAAAAA GGGCTTCGAA 
GACACCAAGT CTCAGTGCCA CGTCTCCCTC GACATGATCC ACCTGGCGCA CGCCCGCCAG 
GCTCAGCACT TAGCCACAGA CATAGGCTAC AGAACAGTGT CCCATCACTT CACAGCGTTG 
CCCACGGACA TGAAGGTGGA GTGGGCCAAG AAGGCGTATG GCTTGCAGAG TGATAACCAA 
TACAGGGCAG ATGTGAAGTG GATGAAAGGC ACGGGCTGGG TCGCCACCGG GTCATTAAAT 
GTGGAGCAGG CAAAGAAGGC AGGAGAACTC ATTAGCGAGA AGAAGTACCG TCAGCATCCA 
GATGCTTTGA AGTTTACCAG TATTAAAGAC ACTCCGGAGA TGGTCCAGGC CAGAATTAGT 
TATACCCAAG CAGTGGATAG GCTGTACAGG GAACAAGGAG AGAACATAAA GCATCATTAC 
ACGCAGACCA CAGACCTGCC TGAGGTCCTG CTGGCCAAGC TGAATGCCAT GAACATCAGC 
GAGACACGGT ATAAGGAATC CTGGAGTAAA CTTCGAGACG GTGGATATAA ACTAAGGTTG 
GATGCCATTC CGTTCCAGGC AGCAAAGGCT TCTAGTGAAA TCATAAGTGA TTACAAATAC 
AAAGAGGCAT TTGAGAAAAT GAAAGGACAG ATGCTTGGCT CCCGGAGCTT GGAAGATGAT 
CTCAGCCTTG CACATTCAGT GCACGCGACC TCACTGCAGA GTGATGTGAA TTACAAGAAA 
GGCTTTGAAC ACTCAAAGGC ACACTTCCAT CTGCCGTTGG ACATGGTAAC CCTGGTGCAC 
GCCAAGAAGG CTCAGACCCT GGCCAGCGAC CAGGACTACA GACATCCGCT CCCCCAGCAC 
ACGTCCTTGG CAGAAGACCT GAGGCTGCGC TGTGCCAAGA AAGCTCACAA GTTGCAGAGC 
GAGAATTTGT ACCGCTCAGA CCTGAACTTC ATGCGAGGCG TTGCCTGCGT CGTTCCAGGC 
ACGTTGGAGA TTGAAGGAAG AAAGAGGGCA TCTGAGCTCA TCAGTGAGAG TAAATACCGT 
CAGCATCCTG GGTCGATCAA GTACACGACT GTGACGGACA CCCCCAACCT CCTTCATGCA 
AAGTTCAGCA ACCAGATTAC CAATGAGCGT CTCTATAAAG CAGCTGGAGA GGATGCTAGA 
CACCAGTACA CAATGACCCT GGGTCTGCCC GAGTTTGTCC GAGCAAAAAC AAATGCGGCC 
AATCTGAGCG ACGCAAAATA CAAGGAGTCT TGGCATGATC TCCGTGCTCA AGGCTACAAG 
CTGACAATAG ATGCGCTCCC CTTCCAGGCT GCAAGGGCCT CTGGGGACAT CGCCAGCGAT 
TTTCTCTACA GACACGACTT TGTGAAGGAG CGAGGGAAGC TCATCGGGAT CCGCAGCGTG 
AGCGATGACC CCCGGCTGCG GCACTGTCGG CTCATGGGCC AGCTGCAAAG TGAGCATCAG 
TACAAGAGGG AGGCAGCCGG CAGCCAAGCC CAGTGCCACC TACCCATGGA CATGGTGCAC 
CTGGTCCATG CCAGGAAGGC CCAGGCCCTG GCCAGTGACC ATGACTACAG GACTCAGTAC 
CACGAGTTCA CGGCGCTACC CGAAGACTTG AAGTTGGCCT GGGCCAAGAA AGCCCACGCC 
CTGCAGAGTG AGTTTCGCTA CAAGTCGGAC CTCATGGCCA TGAAGGGGAC AGGATGGCTA 
GCACTGAGAT CCCCACAGAT AGAGAGCGCC AGGAAGGCGG GAGAACTCAT CAGCGAGACC 
AAATACCGTA AAAAACCAGA CAGTATCAAG TTCACCACAG TGGTTGACTC CCCAGATCTG 
GTTCACGCCA AGAATAGCTA CGTGCATTGC AACGAGCGCC TGTACAGGTT GGGGGATGCA 
GAGGCCCTGC ACAGATACAC CCTGATCCCC GACCACCCTG AGTTCACCCG AGCCCGCCTC 
AACGCGCTGC ACCTGAGTGA CAAAGTCTAC AGAAACTCCT GGGAGCAGAC CCGCGCTGGC 
GGCTACGACT TCAGGCTGGA CGCCATCCCG TTCCAGACTG CCCGGGCATC CAGGGAGATC 
GCCAGCGACT TCCGGTATAA AGAGGCTTTC CTGCGGGACC GGGGCCTGCA GATCGGCTTC 
CGCAGCATCA ACGATGACCC GAGGATGAAG CATTTCCTGA ACGTCGGCAG GCTCCAGAGT 
GACAATGAGT ACAAGAAGGA CTTTGCCAAG AGTCGGTCCC AGTTCCACAG CCGCACAGAC 
CAGCCTGGCT TCCTCCAGGC CAAGAGGAGC CAGCAGTTGG CCAGCGACGT GCATTACCGG 
CAGCCCCTGC CCCAGCCCAC GTGCGACCCC GAGCAGCTGG GCCTCAAGCA CGCGCAGAAG 
GCCCACCAGC TGCAGAGTGA CGTCAAGTAC AAATCAGACT TGAACCTGAC CAGGGGTGTC 
GGCTGGACCC CTCCCGGCTC CTACAAAGTG GAAATGGCTC GGCGGGCTGC AGAACTGGCC 
AACATGAGGG GCCTGGGGCT CCCGGGGGCT TATGGGGAGC CGGAGGTGGT GGAGGCTGGA 
GGTCACCCGA GGGGGGAGGT GAACCCAGAT GCCACTGAGA TTCTGCACGT CAAAAGGAAA 
AAGGCTCTGT GGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_013356570.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013212024.1 Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X3, mRNA.

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CloneID	OIc52003
Clone ID Related Accession (Same CDS sequence)	XM_013356570.1 , XM_013356570.2
Accession Version	XM_013356570.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTT 
GCCTACAAGG CTGATTACAA ACACGATATT GTGGACTACA ACTATCCAGC CACCCTTACT 
CCTGCCTATC AAACAACAAT GAAACTGGTT CCTTTGAAAG ATGTCAACTA CAGGCAAAGC 
ATCGACAAGC TGAAGTACAG CTCTGTGACC AATACTCCGC AGATTGTTCA GGCCAAAATC 
AATGCCCAGC AACTGAGCCA CGTGAACTAC CGCGCTGACT ATGAGAAAAA CAAGTTGAAT 
TACACACTGC CGCAGGATGT GCCTCAGCTG GTGAAGGCCA GAACTAACGC TGAACTCTTC 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGACAAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCCA TGTCTCTGTT GGCCGCCAAA GCCTCGGGGG AGCTTGCTAG CAATATTAAA 
TACAAAGAAG AATATGAGAA AACAAAAGGC AAAGCCATGG GAACAACCGA CTCGAAGCTT 
CTGCACTCCC TGCAGGTGGC CAAGATGAGC AGCGAGGTTG AATACAAGAA AGGCTTTGAG 
GAGAGTAAGA CCCACTTTCA CCTGCCCATG GATATGGTAA ACATCAGGCA TGCTAAGAAG 
GCCCAAGCTC TTGCCAGTGA CCTGGATTAC AGGAAGAAAC TGCACGAATA CACCGTGCTG 
CCTGAAGACA TGAAGACTCA GTGGGCCAAG AAAGCCTACG GGCTCCAGAG CGAGCTGCAG 
TACAAATCCG ACCTGGCGTG GATGAAGGGA GTCGGGTGGC TGACTGAGGG CAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGACAACTG GTCAGCGAGA AAAACTACCG GCAGAGGGTT 
GATGAGCTGA AGTTCACCAG TGTGACCGAT AGTGCGCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGAGAACC AGCAGTCTAT CCACCAGTAC 
ACCATCAGCA AAGACGAGCC TCTCTTCCTG CAGGCCCGGG CCAATGCTGC TCACCTCAGC 
GAGAAACGGT ACAAGAGCAG CTGGGAAAAC CAGAAGGCAA GAGGGTTTGA GCTGAGTCTT 
GATTCCTTGA CCTTCCTGGC AGCCAAAGCC AAGAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAGGACT ATGAGAAGTC CAGAGGGAAG CTCATCGGGG CCAAGGGTGC ACAGGGCGAT 
TCTCAAATGA GCCACTCGCT GCAAATGTCC AAACTGCAGA GCGAATTGGA GTACAAAAAG 
GGCTTCGAAG ACACCAAGTC TCAGTGCCAC GTCTCCCTCG ACATGATCCA CCTGGCGCAC 
GCCCGCCAGG CTCAGCACTT AGCCACAGAC ATAGGCTACA GAACAGTGTC CCATCACTTC 
ACAGCGTTGC CCACGGACAT GAAGGTGGAG TGGGCCAAGA AGGCGTATGG CTTGCAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC AAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG CTGTACAGGG AACAAGGAGA GAACATAAAG 
CATCATTACA CGCAGACCAC AGACCTGCCT GAGGTCCTGC TGGCCAAGCT GAATGCCATG 
AACATCAGCG AGACACGGTA TAAGGAATCC TGGAGTAAAC TTCGAGACGG TGGATATAAA 
CTAAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTAGTGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGACAGA TGCTTGGCTC CCGGAGCTTG 
GAAGATGATC TCAGCCTTGC ACATTCAGTG CACGCGACCT CACTGCAGAG TGATGTGAAT 
TACAAGAAAG GCTTTGAACA CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCACG CCAAGAAGGC TCAGACCCTG GCCAGCGACC AGGACTACAG ACATCCGCTC 
CCCCAGCACA CGTCCTTGGC AGAAGACCTG AGGCTGCGCT GTGCCAAGAA AGCTCACAAG 
TTGCAGAGCG AGAATTTGTA CCGCTCAGAC CTGAACTTCA TGCGAGGCGT TGCCTGCGTC 
GTTCCAGGCA CGTTGGAGAT TGAAGGAAGA AAGAGGGCAT CTGAGCTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCTGG GTCGATCAAG TACACGACTG TGACGGACAC CCCCAACCTC 
CTTCATGCAA AGTTCAGCAA CCAGATTACC AATGAGCGTC TCTATAAAGC AGCTGGAGAG 
GATGCTAGAC ACCAGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCAAAAACA 
AATGCGGCCA ATCTGAGCGA CGCAAAATAC AAGGAGTCTT GGCATGATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA TGCGCTCCCC TTCCAGGCTG CAAGGGCCTC TGGGGACATC 
GCCAGCGATT TTCTCTACAG ACACGACTTT GTGAAGGAGC GAGGGAAGCT CATCGGGATC 
CGCAGCGTGA GCGATGACCC CCGGCTGCGG CACTGTCGGC TCATGGGCCA GCTGCAAAGT 
GAGCATCAGT ACAAGAGGGA GGCAGCCGGC AGCCAAGCCC AGTGCCACCT ACCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTGG CCAGTGACCA TGACTACAGG 
ACTCAGTACC ACGAGTTCAC GGCGCTACCC GAAGACTTGA AGTTGGCCTG GGCCAAGAAA 
GCCCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCGGACC TCATGGCCAT GAAGGGGACA 
GGATGGCTAG CACTGAGATC CCCACAGATA GAGAGCGCCA GGAAGGCGGG AGAACTCATC 
AGCGAGACCA AATACCGTAA AAAACCAGAC AGTATCAAGT TCACCACAGT GGTTGACTCC 
CCAGATCTGG TTCACGCCAA GAATAGCTAC GTGCATTGCA ACGAGCGCCT GTACAGGTTG 
GGGGATGCAG AGGCCCTGCA CAGATACACC CTGATCCCCG ACCACCCTGA GTTCACCCGA 
GCCCGCCTCA ACGCGCTGCA CCTGAGTGAC AAAGTCTACA GAAACTCCTG GGAGCAGACC 
CGCGCTGGCG GCTACGACTT CAGGCTGGAC GCCATCCCGT TCCAGACTGC CCGGGCATCC 
AGGGAGATCG CCAGCGACTT CCGGTATAAA GAGGCTTTCC TGCGGGACCG GGGCCTGCAG 
ATCGGCTTCC GCAGCATCAA CGATGACCCG AGGATGAAGC ATTTCCTGAA CGTCGGCAGG 
CTCCAGAGTG ACAATGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCACAGC 
CGCACAGACC AGCCTGGCTT CCTCCAGGCC AAGAGGAGCC AGCAGTTGGC CAGCGACGTG 
CATTACCGGC AGCCCCTGCC CCAGCCCACG TGCGACCCCG AGCAGCTGGG CCTCAAGCAC 
GCGCAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGGGGTGTCG GCTGGACCCC TCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCTGCA 
GAACTGGCCA ACATGAGGGG CCTGGGGCTC CCGGGGGCTT ATGGGGAGCC GGAGGTGGTG 
GAGGCTGGAG GTCACCCGAG GGGGGAGGTG AACCCAGATG CCACTGAGAT TCTGCACGTC 
AAAAGGAAAA AGGCTCTGTG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013212024.1
CDS	205..5151
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_013356570.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin-related anchoring protein (Nrap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356570.1

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTT 
GCCTACAAGG CTGATTACAA ACACGATATT GTGGACTACA ACTATCCAGC CACCCTTACT 
CCTGCCTATC AAACAACAAT GAAACTGGTT CCTTTGAAAG ATGTCAACTA CAGGCAAAGC 
ATCGACAAGC TGAAGTACAG CTCTGTGACC AATACTCCGC AGATTGTTCA GGCCAAAATC 
AATGCCCAGC AACTGAGCCA CGTGAACTAC CGCGCTGACT ATGAGAAAAA CAAGTTGAAT 
TACACACTGC CGCAGGATGT GCCTCAGCTG GTGAAGGCCA GAACTAACGC TGAACTCTTC 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGACAAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCCA TGTCTCTGTT GGCCGCCAAA GCCTCGGGGG AGCTTGCTAG CAATATTAAA 
TACAAAGAAG AATATGAGAA AACAAAAGGC AAAGCCATGG GAACAACCGA CTCGAAGCTT 
CTGCACTCCC TGCAGGTGGC CAAGATGAGC AGCGAGGTTG AATACAAGAA AGGCTTTGAG 
GAGAGTAAGA CCCACTTTCA CCTGCCCATG GATATGGTAA ACATCAGGCA TGCTAAGAAG 
GCCCAAGCTC TTGCCAGTGA CCTGGATTAC AGGAAGAAAC TGCACGAATA CACCGTGCTG 
CCTGAAGACA TGAAGACTCA GTGGGCCAAG AAAGCCTACG GGCTCCAGAG CGAGCTGCAG 
TACAAATCCG ACCTGGCGTG GATGAAGGGA GTCGGGTGGC TGACTGAGGG CAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGACAACTG GTCAGCGAGA AAAACTACCG GCAGAGGGTT 
GATGAGCTGA AGTTCACCAG TGTGACCGAT AGTGCGCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGAGAACC AGCAGTCTAT CCACCAGTAC 
ACCATCAGCA AAGACGAGCC TCTCTTCCTG CAGGCCCGGG CCAATGCTGC TCACCTCAGC 
GAGAAACGGT ACAAGAGCAG CTGGGAAAAC CAGAAGGCAA GAGGGTTTGA GCTGAGTCTT 
GATTCCTTGA CCTTCCTGGC AGCCAAAGCC AAGAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAGGACT ATGAGAAGTC CAGAGGGAAG CTCATCGGGG CCAAGGGTGC ACAGGGCGAT 
TCTCAAATGA GCCACTCGCT GCAAATGTCC AAACTGCAGA GCGAATTGGA GTACAAAAAG 
GGCTTCGAAG ACACCAAGTC TCAGTGCCAC GTCTCCCTCG ACATGATCCA CCTGGCGCAC 
GCCCGCCAGG CTCAGCACTT AGCCACAGAC ATAGGCTACA GAACAGTGTC CCATCACTTC 
ACAGCGTTGC CCACGGACAT GAAGGTGGAG TGGGCCAAGA AGGCGTATGG CTTGCAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC AAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG CTGTACAGGG AACAAGGAGA GAACATAAAG 
CATCATTACA CGCAGACCAC AGACCTGCCT GAGGTCCTGC TGGCCAAGCT GAATGCCATG 
AACATCAGCG AGACACGGTA TAAGGAATCC TGGAGTAAAC TTCGAGACGG TGGATATAAA 
CTAAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTAGTGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGACAGA TGCTTGGCTC CCGGAGCTTG 
GAAGATGATC TCAGCCTTGC ACATTCAGTG CACGCGACCT CACTGCAGAG TGATGTGAAT 
TACAAGAAAG GCTTTGAACA CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCACG CCAAGAAGGC TCAGACCCTG GCCAGCGACC AGGACTACAG ACATCCGCTC 
CCCCAGCACA CGTCCTTGGC AGAAGACCTG AGGCTGCGCT GTGCCAAGAA AGCTCACAAG 
TTGCAGAGCG AGAATTTGTA CCGCTCAGAC CTGAACTTCA TGCGAGGCGT TGCCTGCGTC 
GTTCCAGGCA CGTTGGAGAT TGAAGGAAGA AAGAGGGCAT CTGAGCTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCTGG GTCGATCAAG TACACGACTG TGACGGACAC CCCCAACCTC 
CTTCATGCAA AGTTCAGCAA CCAGATTACC AATGAGCGTC TCTATAAAGC AGCTGGAGAG 
GATGCTAGAC ACCAGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCAAAAACA 
AATGCGGCCA ATCTGAGCGA CGCAAAATAC AAGGAGTCTT GGCATGATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA TGCGCTCCCC TTCCAGGCTG CAAGGGCCTC TGGGGACATC 
GCCAGCGATT TTCTCTACAG ACACGACTTT GTGAAGGAGC GAGGGAAGCT CATCGGGATC 
CGCAGCGTGA GCGATGACCC CCGGCTGCGG CACTGTCGGC TCATGGGCCA GCTGCAAAGT 
GAGCATCAGT ACAAGAGGGA GGCAGCCGGC AGCCAAGCCC AGTGCCACCT ACCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTGG CCAGTGACCA TGACTACAGG 
ACTCAGTACC ACGAGTTCAC GGCGCTACCC GAAGACTTGA AGTTGGCCTG GGCCAAGAAA 
GCCCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCGGACC TCATGGCCAT GAAGGGGACA 
GGATGGCTAG CACTGAGATC CCCACAGATA GAGAGCGCCA GGAAGGCGGG AGAACTCATC 
AGCGAGACCA AATACCGTAA AAAACCAGAC AGTATCAAGT TCACCACAGT GGTTGACTCC 
CCAGATCTGG TTCACGCCAA GAATAGCTAC GTGCATTGCA ACGAGCGCCT GTACAGGTTG 
GGGGATGCAG AGGCCCTGCA CAGATACACC CTGATCCCCG ACCACCCTGA GTTCACCCGA 
GCCCGCCTCA ACGCGCTGCA CCTGAGTGAC AAAGTCTACA GAAACTCCTG GGAGCAGACC 
CGCGCTGGCG GCTACGACTT CAGGCTGGAC GCCATCCCGT TCCAGACTGC CCGGGCATCC 
AGGGAGATCG CCAGCGACTT CCGGTATAAA GAGGCTTTCC TGCGGGACCG GGGCCTGCAG 
ATCGGCTTCC GCAGCATCAA CGATGACCCG AGGATGAAGC ATTTCCTGAA CGTCGGCAGG 
CTCCAGAGTG ACAATGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCACAGC 
CGCACAGACC AGCCTGGCTT CCTCCAGGCC AAGAGGAGCC AGCAGTTGGC CAGCGACGTG 
CATTACCGGC AGCCCCTGCC CCAGCCCACG TGCGACCCCG AGCAGCTGGG CCTCAAGCAC 
GCGCAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGGGGTGTCG GCTGGACCCC TCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCTGCA 
GAACTGGCCA ACATGAGGGG CCTGGGGCTC CCGGGGGCTT ATGGGGAGCC GGAGGTGGTG 
GAGGCTGGAG GTCACCCGAG GGGGGAGGTG AACCCAGATG CCACTGAGAT TCTGCACGTC 
AAAAGGAAAA AGGCTCTGTG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_013356570.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013212024.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X6, mRNA.

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CloneID	OIc52003
Clone ID Related Accession (Same CDS sequence)	XM_013356570.1 , XM_013356570.2
Accession Version	XM_013356570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013356570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTT 
GCCTACAAGG CTGATTACAA ACACGATATT GTGGACTACA ACTATCCAGC CACCCTTACT 
CCTGCCTATC AAACAACAAT GAAACTGGTT CCTTTGAAAG ATGTCAACTA CAGGCAAAGC 
ATCGACAAGC TGAAGTACAG CTCTGTGACC AATACTCCGC AGATTGTTCA GGCCAAAATC 
AATGCCCAGC AACTGAGCCA CGTGAACTAC CGCGCTGACT ATGAGAAAAA CAAGTTGAAT 
TACACACTGC CGCAGGATGT GCCTCAGCTG GTGAAGGCCA GAACTAACGC TGAACTCTTC 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGACAAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCCA TGTCTCTGTT GGCCGCCAAA GCCTCGGGGG AGCTTGCTAG CAATATTAAA 
TACAAAGAAG AATATGAGAA AACAAAAGGC AAAGCCATGG GAACAACCGA CTCGAAGCTT 
CTGCACTCCC TGCAGGTGGC CAAGATGAGC AGCGAGGTTG AATACAAGAA AGGCTTTGAG 
GAGAGTAAGA CCCACTTTCA CCTGCCCATG GATATGGTAA ACATCAGGCA TGCTAAGAAG 
GCCCAAGCTC TTGCCAGTGA CCTGGATTAC AGGAAGAAAC TGCACGAATA CACCGTGCTG 
CCTGAAGACA TGAAGACTCA GTGGGCCAAG AAAGCCTACG GGCTCCAGAG CGAGCTGCAG 
TACAAATCCG ACCTGGCGTG GATGAAGGGA GTCGGGTGGC TGACTGAGGG CAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGACAACTG GTCAGCGAGA AAAACTACCG GCAGAGGGTT 
GATGAGCTGA AGTTCACCAG TGTGACCGAT AGTGCGCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGAGAACC AGCAGTCTAT CCACCAGTAC 
ACCATCAGCA AAGACGAGCC TCTCTTCCTG CAGGCCCGGG CCAATGCTGC TCACCTCAGC 
GAGAAACGGT ACAAGAGCAG CTGGGAAAAC CAGAAGGCAA GAGGGTTTGA GCTGAGTCTT 
GATTCCTTGA CCTTCCTGGC AGCCAAAGCC AAGAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAGGACT ATGAGAAGTC CAGAGGGAAG CTCATCGGGG CCAAGGGTGC ACAGGGCGAT 
TCTCAAATGA GCCACTCGCT GCAAATGTCC AAACTGCAGA GCGAATTGGA GTACAAAAAG 
GGCTTCGAAG ACACCAAGTC TCAGTGCCAC GTCTCCCTCG ACATGATCCA CCTGGCGCAC 
GCCCGCCAGG CTCAGCACTT AGCCACAGAC ATAGGCTACA GAACAGTGTC CCATCACTTC 
ACAGCGTTGC CCACGGACAT GAAGGTGGAG TGGGCCAAGA AGGCGTATGG CTTGCAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC AAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG CTGTACAGGG AACAAGGAGA GAACATAAAG 
CATCATTACA CGCAGACCAC AGACCTGCCT GAGGTCCTGC TGGCCAAGCT GAATGCCATG 
AACATCAGCG AGACACGGTA TAAGGAATCC TGGAGTAAAC TTCGAGACGG TGGATATAAA 
CTAAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTAGTGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGACAGA TGCTTGGCTC CCGGAGCTTG 
GAAGATGATC TCAGCCTTGC ACATTCAGTG CACGCGACCT CACTGCAGAG TGATGTGAAT 
TACAAGAAAG GCTTTGAACA CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCACG CCAAGAAGGC TCAGACCCTG GCCAGCGACC AGGACTACAG ACATCCGCTC 
CCCCAGCACA CGTCCTTGGC AGAAGACCTG AGGCTGCGCT GTGCCAAGAA AGCTCACAAG 
TTGCAGAGCG AGAATTTGTA CCGCTCAGAC CTGAACTTCA TGCGAGGCGT TGCCTGCGTC 
GTTCCAGGCA CGTTGGAGAT TGAAGGAAGA AAGAGGGCAT CTGAGCTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCTGG GTCGATCAAG TACACGACTG TGACGGACAC CCCCAACCTC 
CTTCATGCAA AGTTCAGCAA CCAGATTACC AATGAGCGTC TCTATAAAGC AGCTGGAGAG 
GATGCTAGAC ACCAGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCAAAAACA 
AATGCGGCCA ATCTGAGCGA CGCAAAATAC AAGGAGTCTT GGCATGATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA TGCGCTCCCC TTCCAGGCTG CAAGGGCCTC TGGGGACATC 
GCCAGCGATT TTCTCTACAG ACACGACTTT GTGAAGGAGC GAGGGAAGCT CATCGGGATC 
CGCAGCGTGA GCGATGACCC CCGGCTGCGG CACTGTCGGC TCATGGGCCA GCTGCAAAGT 
GAGCATCAGT ACAAGAGGGA GGCAGCCGGC AGCCAAGCCC AGTGCCACCT ACCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTGG CCAGTGACCA TGACTACAGG 
ACTCAGTACC ACGAGTTCAC GGCGCTACCC GAAGACTTGA AGTTGGCCTG GGCCAAGAAA 
GCCCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCGGACC TCATGGCCAT GAAGGGGACA 
GGATGGCTAG CACTGAGATC CCCACAGATA GAGAGCGCCA GGAAGGCGGG AGAACTCATC 
AGCGAGACCA AATACCGTAA AAAACCAGAC AGTATCAAGT TCACCACAGT GGTTGACTCC 
CCAGATCTGG TTCACGCCAA GAATAGCTAC GTGCATTGCA ACGAGCGCCT GTACAGGTTG 
GGGGATGCAG AGGCCCTGCA CAGATACACC CTGATCCCCG ACCACCCTGA GTTCACCCGA 
GCCCGCCTCA ACGCGCTGCA CCTGAGTGAC AAAGTCTACA GAAACTCCTG GGAGCAGACC 
CGCGCTGGCG GCTACGACTT CAGGCTGGAC GCCATCCCGT TCCAGACTGC CCGGGCATCC 
AGGGAGATCG CCAGCGACTT CCGGTATAAA GAGGCTTTCC TGCGGGACCG GGGCCTGCAG 
ATCGGCTTCC GCAGCATCAA CGATGACCCG AGGATGAAGC ATTTCCTGAA CGTCGGCAGG 
CTCCAGAGTG ACAATGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCACAGC 
CGCACAGACC AGCCTGGCTT CCTCCAGGCC AAGAGGAGCC AGCAGTTGGC CAGCGACGTG 
CATTACCGGC AGCCCCTGCC CCAGCCCACG TGCGACCCCG AGCAGCTGGG CCTCAAGCAC 
GCGCAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGGGGTGTCG GCTGGACCCC TCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCTGCA 
GAACTGGCCA ACATGAGGGG CCTGGGGCTC CCGGGGGCTT ATGGGGAGCC GGAGGTGGTG 
GAGGCTGGAG GTCACCCGAG GGGGGAGGTG AACCCAGATG CCACTGAGAT TCTGCACGTC 
AAAAGGAAAA AGGCTCTGTG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013212024.1
CDS	237..5183
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_013356570.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356570.2

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTT 
GCCTACAAGG CTGATTACAA ACACGATATT GTGGACTACA ACTATCCAGC CACCCTTACT 
CCTGCCTATC AAACAACAAT GAAACTGGTT CCTTTGAAAG ATGTCAACTA CAGGCAAAGC 
ATCGACAAGC TGAAGTACAG CTCTGTGACC AATACTCCGC AGATTGTTCA GGCCAAAATC 
AATGCCCAGC AACTGAGCCA CGTGAACTAC CGCGCTGACT ATGAGAAAAA CAAGTTGAAT 
TACACACTGC CGCAGGATGT GCCTCAGCTG GTGAAGGCCA GAACTAACGC TGAACTCTTC 
AGTGAGGTTA AGTACAAAGA AGGCTGGGAG AAGACAAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCCA TGTCTCTGTT GGCCGCCAAA GCCTCGGGGG AGCTTGCTAG CAATATTAAA 
TACAAAGAAG AATATGAGAA AACAAAAGGC AAAGCCATGG GAACAACCGA CTCGAAGCTT 
CTGCACTCCC TGCAGGTGGC CAAGATGAGC AGCGAGGTTG AATACAAGAA AGGCTTTGAG 
GAGAGTAAGA CCCACTTTCA CCTGCCCATG GATATGGTAA ACATCAGGCA TGCTAAGAAG 
GCCCAAGCTC TTGCCAGTGA CCTGGATTAC AGGAAGAAAC TGCACGAATA CACCGTGCTG 
CCTGAAGACA TGAAGACTCA GTGGGCCAAG AAAGCCTACG GGCTCCAGAG CGAGCTGCAG 
TACAAATCCG ACCTGGCGTG GATGAAGGGA GTCGGGTGGC TGACTGAGGG CAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGACAACTG GTCAGCGAGA AAAACTACCG GCAGAGGGTT 
GATGAGCTGA AGTTCACCAG TGTGACCGAT AGTGCGCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGAGAACC AGCAGTCTAT CCACCAGTAC 
ACCATCAGCA AAGACGAGCC TCTCTTCCTG CAGGCCCGGG CCAATGCTGC TCACCTCAGC 
GAGAAACGGT ACAAGAGCAG CTGGGAAAAC CAGAAGGCAA GAGGGTTTGA GCTGAGTCTT 
GATTCCTTGA CCTTCCTGGC AGCCAAAGCC AAGAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAGGACT ATGAGAAGTC CAGAGGGAAG CTCATCGGGG CCAAGGGTGC ACAGGGCGAT 
TCTCAAATGA GCCACTCGCT GCAAATGTCC AAACTGCAGA GCGAATTGGA GTACAAAAAG 
GGCTTCGAAG ACACCAAGTC TCAGTGCCAC GTCTCCCTCG ACATGATCCA CCTGGCGCAC 
GCCCGCCAGG CTCAGCACTT AGCCACAGAC ATAGGCTACA GAACAGTGTC CCATCACTTC 
ACAGCGTTGC CCACGGACAT GAAGGTGGAG TGGGCCAAGA AGGCGTATGG CTTGCAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC AAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG CTGTACAGGG AACAAGGAGA GAACATAAAG 
CATCATTACA CGCAGACCAC AGACCTGCCT GAGGTCCTGC TGGCCAAGCT GAATGCCATG 
AACATCAGCG AGACACGGTA TAAGGAATCC TGGAGTAAAC TTCGAGACGG TGGATATAAA 
CTAAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTAGTGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGACAGA TGCTTGGCTC CCGGAGCTTG 
GAAGATGATC TCAGCCTTGC ACATTCAGTG CACGCGACCT CACTGCAGAG TGATGTGAAT 
TACAAGAAAG GCTTTGAACA CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCACG CCAAGAAGGC TCAGACCCTG GCCAGCGACC AGGACTACAG ACATCCGCTC 
CCCCAGCACA CGTCCTTGGC AGAAGACCTG AGGCTGCGCT GTGCCAAGAA AGCTCACAAG 
TTGCAGAGCG AGAATTTGTA CCGCTCAGAC CTGAACTTCA TGCGAGGCGT TGCCTGCGTC 
GTTCCAGGCA CGTTGGAGAT TGAAGGAAGA AAGAGGGCAT CTGAGCTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCTGG GTCGATCAAG TACACGACTG TGACGGACAC CCCCAACCTC 
CTTCATGCAA AGTTCAGCAA CCAGATTACC AATGAGCGTC TCTATAAAGC AGCTGGAGAG 
GATGCTAGAC ACCAGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCAAAAACA 
AATGCGGCCA ATCTGAGCGA CGCAAAATAC AAGGAGTCTT GGCATGATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA TGCGCTCCCC TTCCAGGCTG CAAGGGCCTC TGGGGACATC 
GCCAGCGATT TTCTCTACAG ACACGACTTT GTGAAGGAGC GAGGGAAGCT CATCGGGATC 
CGCAGCGTGA GCGATGACCC CCGGCTGCGG CACTGTCGGC TCATGGGCCA GCTGCAAAGT 
GAGCATCAGT ACAAGAGGGA GGCAGCCGGC AGCCAAGCCC AGTGCCACCT ACCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTGG CCAGTGACCA TGACTACAGG 
ACTCAGTACC ACGAGTTCAC GGCGCTACCC GAAGACTTGA AGTTGGCCTG GGCCAAGAAA 
GCCCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCGGACC TCATGGCCAT GAAGGGGACA 
GGATGGCTAG CACTGAGATC CCCACAGATA GAGAGCGCCA GGAAGGCGGG AGAACTCATC 
AGCGAGACCA AATACCGTAA AAAACCAGAC AGTATCAAGT TCACCACAGT GGTTGACTCC 
CCAGATCTGG TTCACGCCAA GAATAGCTAC GTGCATTGCA ACGAGCGCCT GTACAGGTTG 
GGGGATGCAG AGGCCCTGCA CAGATACACC CTGATCCCCG ACCACCCTGA GTTCACCCGA 
GCCCGCCTCA ACGCGCTGCA CCTGAGTGAC AAAGTCTACA GAAACTCCTG GGAGCAGACC 
CGCGCTGGCG GCTACGACTT CAGGCTGGAC GCCATCCCGT TCCAGACTGC CCGGGCATCC 
AGGGAGATCG CCAGCGACTT CCGGTATAAA GAGGCTTTCC TGCGGGACCG GGGCCTGCAG 
ATCGGCTTCC GCAGCATCAA CGATGACCCG AGGATGAAGC ATTTCCTGAA CGTCGGCAGG 
CTCCAGAGTG ACAATGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCACAGC 
CGCACAGACC AGCCTGGCTT CCTCCAGGCC AAGAGGAGCC AGCAGTTGGC CAGCGACGTG 
CATTACCGGC AGCCCCTGCC CCAGCCCACG TGCGACCCCG AGCAGCTGGG CCTCAAGCAC 
GCGCAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGGGGTGTCG GCTGGACCCC TCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCTGCA 
GAACTGGCCA ACATGAGGGG CCTGGGGCTC CCGGGGGCTT ATGGGGAGCC GGAGGTGGTG 
GAGGCTGGAG GTCACCCGAG GGGGGAGGTG AACCCAGATG CCACTGAGAT TCTGCACGTC 
AAAAGGAAAA AGGCTCTGTG GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_021723328.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021579003.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X2, mRNA.

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CloneID	OIc76024
Clone ID Related Accession (Same CDS sequence)	XM_021723328.1
Accession Version	XM_021723328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTG 
GAATATAAGA AGGATCTGGA AAGTAGTAAA GGTCACAGTA TCAACTACTG TGAAACGCCT 
CAGTTCAGGA ACGTGAGCAA GATCTCAAAA TTCACCAGTG ATAATAAATA TAAAGAAAAC 
TACCAGAACC ACATGAGAGG CCACTATGAA GGAGTTGGCA TGGACAGACG CACACTTCAT 
GCTCTGAAAG TTGGCAGTCT GGCCAGCAAT GTTGCCTACA AGGCTGATTA CAAACACGAT 
ATTGTGGACT ACAACTATCC AGCCACCCTT ACTCCTGCCT ATCAAACAAC AATGAAACTG 
GTTCCTTTGA AAGATGTCAA CTACAGGCAA AGCATCGACA AGCTGAAGTA CAGCTCTGTG 
ACCAATACTC CGCAGATTGT TCAGGCCAAA ATCAATGCCC AGCAACTGAG CCACGTGAAC 
TACCGCGCTG ACTATGAGAA AAACAAGTTG AATTACACAC TGCCGCAGGA TGTGCCTCAG 
CTGGTGAAGG CCAGAACTAA CGCTGAACTC TTCAGTGAGG TTAAGTACAA AGAAGGCTGG 
GAGAAGACAA AGGGGAAAGG ATTTGAGATG AAGCTGGATG CCATGTCTCT GTTGGCCGCC 
AAAGCCTCGG GGGAGCTTGC TAGCAATATT AAATACAAAG AAGAATATGA GAAAACAAAA 
GGCAAAGCCA TGGGAACAAC CGACTCGAAG CTTCTGCACT CCCTGCAGGT GGCCAAGATG 
AGCAGCGAGG TTGAATACAA GAAAGGCTTT GAGGAGAGTA AGACCCACTT TCACCTGCCC 
ATGGATATGG TAAACATCAG GCATGCTAAG AAGGCCCAAG CTCTTGCCAG TGACCTGGAT 
TACAGGAAGA AACTGCACGA ATACACCGTG CTGCCTGAAG ACATGAAGAC TCAGTGGGCC 
AAGAAAGCCT ACGGGCTCCA GAGCGAGCTG CAGTACAAAT CCGACCTGGC GTGGATGAAG 
GGAGTCGGGT GGCTGACTGA GGGCAGTCTC AATTTGGAAC AGGCCAAGAA GGCTGGACAA 
CTGGTCAGCG AGAAAAACTA CCGGCAGAGG GTTGATGAGC TGAAGTTCAC CAGTGTGACC 
GATAGTGCGC AGATGGAGCA CGCCAAGAAG AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC 
AAGGCAGAGA ACCAGCAGTC TATCCACCAG TACACCATCA GCAAAGACGA GCCTCTCTTC 
CTGCAGGCCC GGGCCAATGC TGCTCACCTC AGCGAGAAAC GGTACAAGAG CAGCTGGGAA 
AACCAGAAGG CAAGAGGGTT TGAGCTGAGT CTTGATTCCT TGACCTTCCT GGCAGCCAAA 
GCCAAGAGGG ACCTGGCGAG CGAGGTGAAG TACAAGGAGG ACTATGAGAA GTCCAGAGGG 
AAGCTCATCG GGGCCAAGGG TGCACAGGGC GATTCTCAAA TGAGCCACTC GCTGCAAATG 
TCCAAACTGC AGAGCGAATT GGAGTACAAA AAGGGCTTCG AAGACACCAA GTCTCAGTGC 
CACGTCTCCC TCGACATGAT CCACCTGGCG CACGCCCGCC AGGCTCAGCA CTTAGCCACA 
GACATAGGCT ACAGAACAGT GTCCCATCAC TTCACAGCGT TGCCCACGGA CATGAAGGTG 
GAGTGGGCCA AGAAGGCGTA TGGCTTGCAG AGTGATAACC AATACAGGGC AGATGTGAAG 
TGGATGAAAG GCACGGGCTG GGTCGCCACC GGGTCATTAA ATGTGGAGCA GGCAAAGAAG 
GCAGGAGAAC TCATTAGCGA GAAGAAGTAC CGTCAGCATC CAGATGCTTT GAAGTTTACC 
AGTATTAAAG ACACTCCGGA GATGGTCCAG GCCAGAATTA GTTATACCCA AGCAGTGGAT 
AGGCTGTACA GGGAACAAGG AGAGAACATA AAGCATCATT ACACGCAGAC CACAGACCTG 
CCTGAGGTCC TGCTGGCCAA GCTGAATGCC ATGAACATCA GCGAGACACG GTATAAGGAA 
TCCTGGAGTA AACTTCGAGA CGGTGGATAT AAACTAAGGT TGGATGCCAT TCCGTTCCAG 
GCAGCAAAGG CTTCTAGTGA AATCATAAGT GATTACAAAT ACAAAGAGGC ATTTGAGAAA 
ATGAAAGGAC AGATGCTTGG CTCCCGGAGC TTGGAAGATG ATCTCAGCCT TGCACATTCA 
GTGCACGCGA CCTCACTGCA GAGTGATGTG AATTACAAGA AAGGCTTTGA ACACTCAAAG 
GCACACTTCC ATCTGCCGTT GGACATGGTA ACCCTGGTGC ACGCCAAGAA GGCTCAGACC 
CTGGCCAGCG ACCAGGACTA CAGACATCCG CTCCCCCAGC ACACGTCCTT GGCAGAAGAC 
CTGAGGCTGC GCTGTGCCAA GAAAGCTCAC AAGTTGCAGA GCGAGAATTT GTACCGCTCA 
GACCTGAACT TCATGCGAGG CGTTGCCTGC GTCGTTCCAG GCACGTTGGA GATTGAAGGA 
AGAAAGAGGG CATCTGAGCT CATCAGTGAG AGTAAATACC GTCAGCATCC TGGGTCGATC 
AAGTACACGA CTGTGACGGA CACCCCCAAC CTCCTTCATG CAAAGTTCAG CAACCAGATT 
ACCAATGAGC GTCTCTATAA AGCAGCTGGA GAGGATGCTA GACACCAGTA CACAATGACC 
CTGGGTCTGC CCGAGTTTGT CCGAGCAAAA ACAAATGCGG CCAATCTGAG CGACGCAAAA 
TACAAGGAGT CTTGGCATGA TCTCCGTGCT CAAGGCTACA AGCTGACAAT AGATGCGCTC 
CCCTTCCAGG CTGCAAGGGC CTCTGGGGAC ATCGCCAGCG ATTTTCTCTA CAGACACGAC 
TTTGTGAAGG AGCGAGGGAA GCTCATCGGG ATCCGCAGCG TGAGCGATGA CCCCCGGCTG 
CGGCACTGTC GGCTCATGGG CCAGCTGCAA AGTGAGCATC AGTACAAGAG GGAGGCAGCC 
GGCAGCCAAG CCCAGTGCCA CCTACCCATG GACATGGTGC ACCTGGTCCA TGCCAGGAAG 
GCCCAGGCCC TGGCCAGTGA CCATGACTAC AGGACTCAGT ACCACGAGTT CACGGCGCTA 
CCCGAAGACT TGAAGTTGGC CTGGGCCAAG AAAGCCCACG CCCTGCAGAG TGAGTTTCGC 
TACAAGTCGG ACCTCATGGC CATGAAGGGG ACAGGATGGC TAGCACTGAG ATCCCCACAG 
ATAGAGAGCG CCAGGAAGGC GGGAGAACTC ATCAGCGAGA CCAAATACCG TAAAAAACCA 
GACAGTATCA AGTTCACCAC AGTGGTTGAC TCCCCAGATC TGGTTCACGC CAAGAATAGC 
TACGTGCATT GCAACGAGCG CCTGTACAGG TTGGGGGATG CAGAGGCCCT GCACAGATAC 
ACCCTGATCC CCGACCACCC TGAGTTCACC CGAGCCCGCC TCAACGCGCT GCACCTGAGT 
GACAAAGTCT ACAGAAACTC CTGGGAGCAG ACCCGCGCTG GCGGCTACGA CTTCAGGCTG 
GACGCCATCC CGTTCCAGAC TGCCCGGGCA TCCAGGGAGA TCGCCAGCGA CTTCCGGTAT 
AAAGAGGCTT TCCTGCGGGA CCGGGGCCTG CAGATCGGCT TCCGCAGCAT CAACGATGAC 
CCGAGGATGA AGCATTTCCT GAACGTCGGC AGGCTCCAGA GTGACAATGA GTACAAGAAG 
GACTTTGCCA AGAGTCGGTC CCAGTTCCAC AGCCGCACAG ACCAGCCTGG CTTCCTCCAG 
GCCAAGAGGA GCCAGCAGTT GGCCAGCGAC GTGCATTACC GGCAGCCCCT GCCCCAGCCC 
ACGTGCGACC CCGAGCAGCT GGGCCTCAAG CACGCGCAGA AGGCCCACCA GCTGCAGAGT 
GACGTCAAGT ACAAATCAGA CTTGAACCTG ACCAGGGGTG TCGGCTGGAC CCCTCCCGGC 
TCCTACAAAG TGGAAATGGC TCGGCGGGCT GCAGAACTGG CCAACATGAG GGGCCTGGGG 
CTCCCGGGGG CTTATGGGGA GCCGGAGGTG GTGGAGGCTG GAGGTCACCC GAGGGGGGAG 
GTGAACCCAG ATGCCACTGA GATTCTGCAC GTCAAAAGGA AAAAGGCTCT GTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021579003.1
CDS	237..5396
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_021723328.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021723328.1

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGGTG 
GAATATAAGA AGGATCTGGA AAGTAGTAAA GGTCACAGTA TCAACTACTG TGAAACGCCT 
CAGTTCAGGA ACGTGAGCAA GATCTCAAAA TTCACCAGTG ATAATAAATA TAAAGAAAAC 
TACCAGAACC ACATGAGAGG CCACTATGAA GGAGTTGGCA TGGACAGACG CACACTTCAT 
GCTCTGAAAG TTGGCAGTCT GGCCAGCAAT GTTGCCTACA AGGCTGATTA CAAACACGAT 
ATTGTGGACT ACAACTATCC AGCCACCCTT ACTCCTGCCT ATCAAACAAC AATGAAACTG 
GTTCCTTTGA AAGATGTCAA CTACAGGCAA AGCATCGACA AGCTGAAGTA CAGCTCTGTG 
ACCAATACTC CGCAGATTGT TCAGGCCAAA ATCAATGCCC AGCAACTGAG CCACGTGAAC 
TACCGCGCTG ACTATGAGAA AAACAAGTTG AATTACACAC TGCCGCAGGA TGTGCCTCAG 
CTGGTGAAGG CCAGAACTAA CGCTGAACTC TTCAGTGAGG TTAAGTACAA AGAAGGCTGG 
GAGAAGACAA AGGGGAAAGG ATTTGAGATG AAGCTGGATG CCATGTCTCT GTTGGCCGCC 
AAAGCCTCGG GGGAGCTTGC TAGCAATATT AAATACAAAG AAGAATATGA GAAAACAAAA 
GGCAAAGCCA TGGGAACAAC CGACTCGAAG CTTCTGCACT CCCTGCAGGT GGCCAAGATG 
AGCAGCGAGG TTGAATACAA GAAAGGCTTT GAGGAGAGTA AGACCCACTT TCACCTGCCC 
ATGGATATGG TAAACATCAG GCATGCTAAG AAGGCCCAAG CTCTTGCCAG TGACCTGGAT 
TACAGGAAGA AACTGCACGA ATACACCGTG CTGCCTGAAG ACATGAAGAC TCAGTGGGCC 
AAGAAAGCCT ACGGGCTCCA GAGCGAGCTG CAGTACAAAT CCGACCTGGC GTGGATGAAG 
GGAGTCGGGT GGCTGACTGA GGGCAGTCTC AATTTGGAAC AGGCCAAGAA GGCTGGACAA 
CTGGTCAGCG AGAAAAACTA CCGGCAGAGG GTTGATGAGC TGAAGTTCAC CAGTGTGACC 
GATAGTGCGC AGATGGAGCA CGCCAAGAAG AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC 
AAGGCAGAGA ACCAGCAGTC TATCCACCAG TACACCATCA GCAAAGACGA GCCTCTCTTC 
CTGCAGGCCC GGGCCAATGC TGCTCACCTC AGCGAGAAAC GGTACAAGAG CAGCTGGGAA 
AACCAGAAGG CAAGAGGGTT TGAGCTGAGT CTTGATTCCT TGACCTTCCT GGCAGCCAAA 
GCCAAGAGGG ACCTGGCGAG CGAGGTGAAG TACAAGGAGG ACTATGAGAA GTCCAGAGGG 
AAGCTCATCG GGGCCAAGGG TGCACAGGGC GATTCTCAAA TGAGCCACTC GCTGCAAATG 
TCCAAACTGC AGAGCGAATT GGAGTACAAA AAGGGCTTCG AAGACACCAA GTCTCAGTGC 
CACGTCTCCC TCGACATGAT CCACCTGGCG CACGCCCGCC AGGCTCAGCA CTTAGCCACA 
GACATAGGCT ACAGAACAGT GTCCCATCAC TTCACAGCGT TGCCCACGGA CATGAAGGTG 
GAGTGGGCCA AGAAGGCGTA TGGCTTGCAG AGTGATAACC AATACAGGGC AGATGTGAAG 
TGGATGAAAG GCACGGGCTG GGTCGCCACC GGGTCATTAA ATGTGGAGCA GGCAAAGAAG 
GCAGGAGAAC TCATTAGCGA GAAGAAGTAC CGTCAGCATC CAGATGCTTT GAAGTTTACC 
AGTATTAAAG ACACTCCGGA GATGGTCCAG GCCAGAATTA GTTATACCCA AGCAGTGGAT 
AGGCTGTACA GGGAACAAGG AGAGAACATA AAGCATCATT ACACGCAGAC CACAGACCTG 
CCTGAGGTCC TGCTGGCCAA GCTGAATGCC ATGAACATCA GCGAGACACG GTATAAGGAA 
TCCTGGAGTA AACTTCGAGA CGGTGGATAT AAACTAAGGT TGGATGCCAT TCCGTTCCAG 
GCAGCAAAGG CTTCTAGTGA AATCATAAGT GATTACAAAT ACAAAGAGGC ATTTGAGAAA 
ATGAAAGGAC AGATGCTTGG CTCCCGGAGC TTGGAAGATG ATCTCAGCCT TGCACATTCA 
GTGCACGCGA CCTCACTGCA GAGTGATGTG AATTACAAGA AAGGCTTTGA ACACTCAAAG 
GCACACTTCC ATCTGCCGTT GGACATGGTA ACCCTGGTGC ACGCCAAGAA GGCTCAGACC 
CTGGCCAGCG ACCAGGACTA CAGACATCCG CTCCCCCAGC ACACGTCCTT GGCAGAAGAC 
CTGAGGCTGC GCTGTGCCAA GAAAGCTCAC AAGTTGCAGA GCGAGAATTT GTACCGCTCA 
GACCTGAACT TCATGCGAGG CGTTGCCTGC GTCGTTCCAG GCACGTTGGA GATTGAAGGA 
AGAAAGAGGG CATCTGAGCT CATCAGTGAG AGTAAATACC GTCAGCATCC TGGGTCGATC 
AAGTACACGA CTGTGACGGA CACCCCCAAC CTCCTTCATG CAAAGTTCAG CAACCAGATT 
ACCAATGAGC GTCTCTATAA AGCAGCTGGA GAGGATGCTA GACACCAGTA CACAATGACC 
CTGGGTCTGC CCGAGTTTGT CCGAGCAAAA ACAAATGCGG CCAATCTGAG CGACGCAAAA 
TACAAGGAGT CTTGGCATGA TCTCCGTGCT CAAGGCTACA AGCTGACAAT AGATGCGCTC 
CCCTTCCAGG CTGCAAGGGC CTCTGGGGAC ATCGCCAGCG ATTTTCTCTA CAGACACGAC 
TTTGTGAAGG AGCGAGGGAA GCTCATCGGG ATCCGCAGCG TGAGCGATGA CCCCCGGCTG 
CGGCACTGTC GGCTCATGGG CCAGCTGCAA AGTGAGCATC AGTACAAGAG GGAGGCAGCC 
GGCAGCCAAG CCCAGTGCCA CCTACCCATG GACATGGTGC ACCTGGTCCA TGCCAGGAAG 
GCCCAGGCCC TGGCCAGTGA CCATGACTAC AGGACTCAGT ACCACGAGTT CACGGCGCTA 
CCCGAAGACT TGAAGTTGGC CTGGGCCAAG AAAGCCCACG CCCTGCAGAG TGAGTTTCGC 
TACAAGTCGG ACCTCATGGC CATGAAGGGG ACAGGATGGC TAGCACTGAG ATCCCCACAG 
ATAGAGAGCG CCAGGAAGGC GGGAGAACTC ATCAGCGAGA CCAAATACCG TAAAAAACCA 
GACAGTATCA AGTTCACCAC AGTGGTTGAC TCCCCAGATC TGGTTCACGC CAAGAATAGC 
TACGTGCATT GCAACGAGCG CCTGTACAGG TTGGGGGATG CAGAGGCCCT GCACAGATAC 
ACCCTGATCC CCGACCACCC TGAGTTCACC CGAGCCCGCC TCAACGCGCT GCACCTGAGT 
GACAAAGTCT ACAGAAACTC CTGGGAGCAG ACCCGCGCTG GCGGCTACGA CTTCAGGCTG 
GACGCCATCC CGTTCCAGAC TGCCCGGGCA TCCAGGGAGA TCGCCAGCGA CTTCCGGTAT 
AAAGAGGCTT TCCTGCGGGA CCGGGGCCTG CAGATCGGCT TCCGCAGCAT CAACGATGAC 
CCGAGGATGA AGCATTTCCT GAACGTCGGC AGGCTCCAGA GTGACAATGA GTACAAGAAG 
GACTTTGCCA AGAGTCGGTC CCAGTTCCAC AGCCGCACAG ACCAGCCTGG CTTCCTCCAG 
GCCAAGAGGA GCCAGCAGTT GGCCAGCGAC GTGCATTACC GGCAGCCCCT GCCCCAGCCC 
ACGTGCGACC CCGAGCAGCT GGGCCTCAAG CACGCGCAGA AGGCCCACCA GCTGCAGAGT 
GACGTCAAGT ACAAATCAGA CTTGAACCTG ACCAGGGGTG TCGGCTGGAC CCCTCCCGGC 
TCCTACAAAG TGGAAATGGC TCGGCGGGCT GCAGAACTGG CCAACATGAG GGGCCTGGGG 
CTCCCGGGGG CTTATGGGGA GCCGGAGGTG GTGGAGGCTG GAGGTCACCC GAGGGGGGAG 
GTGAACCCAG ATGCCACTGA GATTCTGCAC GTCAAAAGGA AAAAGGCTCT GTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_021723329.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021579004.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X4, mRNA.

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CloneID	OIc76025
Clone ID Related Accession (Same CDS sequence)	XM_021723329.1
Accession Version	XM_021723329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGAAT 
AAATATAAAG AAAACTACCA GAACCACATG AGAGGCCACT ATGAAGGAGT TGGCATGGAC 
AGACGCACAC TTCATGCTCT GAAAGTTGGC AGTCTGGCCA GCAATGTTGC CTACAAGGCT 
GATTACAAAC ACGATATTGT GGACTACAAC TATCCAGCCA CCCTTACTCC TGCCTATCAA 
ACAACAATGA AACTGGTTCC TTTGAAAGAT GTCAACTACA GGCAAAGCAT CGACAAGCTG 
AAGTACAGCT CTGTGACCAA TACTCCGCAG ATTGTTCAGG CCAAAATCAA TGCCCAGCAA 
CTGAGCCACG TGAACTACCG CGCTGACTAT GAGAAAAACA AGTTGAATTA CACACTGCCG 
CAGGATGTGC CTCAGCTGGT GAAGGCCAGA ACTAACGCTG AACTCTTCAG TGAGGTTAAG 
TACAAAGAAG GCTGGGAGAA GACAAAGGGG AAAGGATTTG AGATGAAGCT GGATGCCATG 
TCTCTGTTGG CCGCCAAAGC CTCGGGGGAG CTTGCTAGCA ATATTAAATA CAAAGAAGAA 
TATGAGAAAA CAAAAGGCAA AGCCATGGGA ACAACCGACT CGAAGCTTCT GCACTCCCTG 
CAGGTGGCCA AGATGAGCAG CGAGGTTGAA TACAAGAAAG GCTTTGAGGA GAGTAAGACC 
CACTTTCACC TGCCCATGGA TATGGTAAAC ATCAGGCATG CTAAGAAGGC CCAAGCTCTT 
GCCAGTGACC TGGATTACAG GAAGAAACTG CACGAATACA CCGTGCTGCC TGAAGACATG 
AAGACTCAGT GGGCCAAGAA AGCCTACGGG CTCCAGAGCG AGCTGCAGTA CAAATCCGAC 
CTGGCGTGGA TGAAGGGAGT CGGGTGGCTG ACTGAGGGCA GTCTCAATTT GGAACAGGCC 
AAGAAGGCTG GACAACTGGT CAGCGAGAAA AACTACCGGC AGAGGGTTGA TGAGCTGAAG 
TTCACCAGTG TGACCGATAG TGCGCAGATG GAGCACGCCA AGAAGAGCCA GGAGCTGCAG 
AGCGGGGTGG CCTACAAGGC AGAGAACCAG CAGTCTATCC ACCAGTACAC CATCAGCAAA 
GACGAGCCTC TCTTCCTGCA GGCCCGGGCC AATGCTGCTC ACCTCAGCGA GAAACGGTAC 
AAGAGCAGCT GGGAAAACCA GAAGGCAAGA GGGTTTGAGC TGAGTCTTGA TTCCTTGACC 
TTCCTGGCAG CCAAAGCCAA GAGGGACCTG GCGAGCGAGG TGAAGTACAA GGAGGACTAT 
GAGAAGTCCA GAGGGAAGCT CATCGGGGCC AAGGGTGCAC AGGGCGATTC TCAAATGAGC 
CACTCGCTGC AAATGTCCAA ACTGCAGAGC GAATTGGAGT ACAAAAAGGG CTTCGAAGAC 
ACCAAGTCTC AGTGCCACGT CTCCCTCGAC ATGATCCACC TGGCGCACGC CCGCCAGGCT 
CAGCACTTAG CCACAGACAT AGGCTACAGA ACAGTGTCCC ATCACTTCAC AGCGTTGCCC 
ACGGACATGA AGGTGGAGTG GGCCAAGAAG GCGTATGGCT TGCAGAGTGA TAACCAATAC 
AGGGCAGATG TGAAGTGGAT GAAAGGCACG GGCTGGGTCG CCACCGGGTC ATTAAATGTG 
GAGCAGGCAA AGAAGGCAGG AGAACTCATT AGCGAGAAGA AGTACCGTCA GCATCCAGAT 
GCTTTGAAGT TTACCAGTAT TAAAGACACT CCGGAGATGG TCCAGGCCAG AATTAGTTAT 
ACCCAAGCAG TGGATAGGCT GTACAGGGAA CAAGGAGAGA ACATAAAGCA TCATTACACG 
CAGACCACAG ACCTGCCTGA GGTCCTGCTG GCCAAGCTGA ATGCCATGAA CATCAGCGAG 
ACACGGTATA AGGAATCCTG GAGTAAACTT CGAGACGGTG GATATAAACT AAGGTTGGAT 
GCCATTCCGT TCCAGGCAGC AAAGGCTTCT AGTGAAATCA TAAGTGATTA CAAATACAAA 
GAGGCATTTG AGAAAATGAA AGGACAGATG CTTGGCTCCC GGAGCTTGGA AGATGATCTC 
AGCCTTGCAC ATTCAGTGCA CGCGACCTCA CTGCAGAGTG ATGTGAATTA CAAGAAAGGC 
TTTGAACACT CAAAGGCACA CTTCCATCTG CCGTTGGACA TGGTAACCCT GGTGCACGCC 
AAGAAGGCTC AGACCCTGGC CAGCGACCAG GACTACAGAC ATCCGCTCCC CCAGCACACG 
TCCTTGGCAG AAGACCTGAG GCTGCGCTGT GCCAAGAAAG CTCACAAGTT GCAGAGCGAG 
AATTTGTACC GCTCAGACCT GAACTTCATG CGAGGCGTTG CCTGCGTCGT TCCAGGCACG 
TTGGAGATTG AAGGAAGAAA GAGGGCATCT GAGCTCATCA GTGAGAGTAA ATACCGTCAG 
CATCCTGGGT CGATCAAGTA CACGACTGTG ACGGACACCC CCAACCTCCT TCATGCAAAG 
TTCAGCAACC AGATTACCAA TGAGCGTCTC TATAAAGCAG CTGGAGAGGA TGCTAGACAC 
CAGTACACAA TGACCCTGGG TCTGCCCGAG TTTGTCCGAG CAAAAACAAA TGCGGCCAAT 
CTGAGCGACG CAAAATACAA GGAGTCTTGG CATGATCTCC GTGCTCAAGG CTACAAGCTG 
ACAATAGATG CGCTCCCCTT CCAGGCTGCA AGGGCCTCTG GGGACATCGC CAGCGATTTT 
CTCTACAGAC ACGACTTTGT GAAGGAGCGA GGGAAGCTCA TCGGGATCCG CAGCGTGAGC 
GATGACCCCC GGCTGCGGCA CTGTCGGCTC ATGGGCCAGC TGCAAAGTGA GCATCAGTAC 
AAGAGGGAGG CAGCCGGCAG CCAAGCCCAG TGCCACCTAC CCATGGACAT GGTGCACCTG 
GTCCATGCCA GGAAGGCCCA GGCCCTGGCC AGTGACCATG ACTACAGGAC TCAGTACCAC 
GAGTTCACGG CGCTACCCGA AGACTTGAAG TTGGCCTGGG CCAAGAAAGC CCACGCCCTG 
CAGAGTGAGT TTCGCTACAA GTCGGACCTC ATGGCCATGA AGGGGACAGG ATGGCTAGCA 
CTGAGATCCC CACAGATAGA GAGCGCCAGG AAGGCGGGAG AACTCATCAG CGAGACCAAA 
TACCGTAAAA AACCAGACAG TATCAAGTTC ACCACAGTGG TTGACTCCCC AGATCTGGTT 
CACGCCAAGA ATAGCTACGT GCATTGCAAC GAGCGCCTGT ACAGGTTGGG GGATGCAGAG 
GCCCTGCACA GATACACCCT GATCCCCGAC CACCCTGAGT TCACCCGAGC CCGCCTCAAC 
GCGCTGCACC TGAGTGACAA AGTCTACAGA AACTCCTGGG AGCAGACCCG CGCTGGCGGC 
TACGACTTCA GGCTGGACGC CATCCCGTTC CAGACTGCCC GGGCATCCAG GGAGATCGCC 
AGCGACTTCC GGTATAAAGA GGCTTTCCTG CGGGACCGGG GCCTGCAGAT CGGCTTCCGC 
AGCATCAACG ATGACCCGAG GATGAAGCAT TTCCTGAACG TCGGCAGGCT CCAGAGTGAC 
AATGAGTACA AGAAGGACTT TGCCAAGAGT CGGTCCCAGT TCCACAGCCG CACAGACCAG 
CCTGGCTTCC TCCAGGCCAA GAGGAGCCAG CAGTTGGCCA GCGACGTGCA TTACCGGCAG 
CCCCTGCCCC AGCCCACGTG CGACCCCGAG CAGCTGGGCC TCAAGCACGC GCAGAAGGCC 
CACCAGCTGC AGAGTGACGT CAAGTACAAA TCAGACTTGA ACCTGACCAG GGGTGTCGGC 
TGGACCCCTC CCGGCTCCTA CAAAGTGGAA ATGGCTCGGC GGGCTGCAGA ACTGGCCAAC 
ATGAGGGGCC TGGGGCTCCC GGGGGCTTAT GGGGAGCCGG AGGTGGTGGA GGCTGGAGGT 
CACCCGAGGG GGGAGGTGAA CCCAGATGCC ACTGAGATTC TGCACGTCAA AAGGAAAAAG 
GCTCTGTGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021579004.1
CDS	237..5291
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHALKVGSLASNVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_021723329.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021723329.1

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGGGTGA CTTGGAGGAG TATGAGGAGC CCAGGGGAAA GGGCAGCTTC 
CCCGCCATGA TCACCCCAGC TTATCAGAAT GCCAAGAAGG CCAACGAACT TGCAAGTGAT 
ATAAAATACA GGCAGGACCT GCACAAGATG AGGGGCGCCG CGCATTATCA TTCTCTTCCA 
GCCCAAGACA ACCTGGTTCT CAAACGGGCC CAGAGTGTGA ACAAGCTCGT GAGCGAGAAT 
AAATATAAAG AAAACTACCA GAACCACATG AGAGGCCACT ATGAAGGAGT TGGCATGGAC 
AGACGCACAC TTCATGCTCT GAAAGTTGGC AGTCTGGCCA GCAATGTTGC CTACAAGGCT 
GATTACAAAC ACGATATTGT GGACTACAAC TATCCAGCCA CCCTTACTCC TGCCTATCAA 
ACAACAATGA AACTGGTTCC TTTGAAAGAT GTCAACTACA GGCAAAGCAT CGACAAGCTG 
AAGTACAGCT CTGTGACCAA TACTCCGCAG ATTGTTCAGG CCAAAATCAA TGCCCAGCAA 
CTGAGCCACG TGAACTACCG CGCTGACTAT GAGAAAAACA AGTTGAATTA CACACTGCCG 
CAGGATGTGC CTCAGCTGGT GAAGGCCAGA ACTAACGCTG AACTCTTCAG TGAGGTTAAG 
TACAAAGAAG GCTGGGAGAA GACAAAGGGG AAAGGATTTG AGATGAAGCT GGATGCCATG 
TCTCTGTTGG CCGCCAAAGC CTCGGGGGAG CTTGCTAGCA ATATTAAATA CAAAGAAGAA 
TATGAGAAAA CAAAAGGCAA AGCCATGGGA ACAACCGACT CGAAGCTTCT GCACTCCCTG 
CAGGTGGCCA AGATGAGCAG CGAGGTTGAA TACAAGAAAG GCTTTGAGGA GAGTAAGACC 
CACTTTCACC TGCCCATGGA TATGGTAAAC ATCAGGCATG CTAAGAAGGC CCAAGCTCTT 
GCCAGTGACC TGGATTACAG GAAGAAACTG CACGAATACA CCGTGCTGCC TGAAGACATG 
AAGACTCAGT GGGCCAAGAA AGCCTACGGG CTCCAGAGCG AGCTGCAGTA CAAATCCGAC 
CTGGCGTGGA TGAAGGGAGT CGGGTGGCTG ACTGAGGGCA GTCTCAATTT GGAACAGGCC 
AAGAAGGCTG GACAACTGGT CAGCGAGAAA AACTACCGGC AGAGGGTTGA TGAGCTGAAG 
TTCACCAGTG TGACCGATAG TGCGCAGATG GAGCACGCCA AGAAGAGCCA GGAGCTGCAG 
AGCGGGGTGG CCTACAAGGC AGAGAACCAG CAGTCTATCC ACCAGTACAC CATCAGCAAA 
GACGAGCCTC TCTTCCTGCA GGCCCGGGCC AATGCTGCTC ACCTCAGCGA GAAACGGTAC 
AAGAGCAGCT GGGAAAACCA GAAGGCAAGA GGGTTTGAGC TGAGTCTTGA TTCCTTGACC 
TTCCTGGCAG CCAAAGCCAA GAGGGACCTG GCGAGCGAGG TGAAGTACAA GGAGGACTAT 
GAGAAGTCCA GAGGGAAGCT CATCGGGGCC AAGGGTGCAC AGGGCGATTC TCAAATGAGC 
CACTCGCTGC AAATGTCCAA ACTGCAGAGC GAATTGGAGT ACAAAAAGGG CTTCGAAGAC 
ACCAAGTCTC AGTGCCACGT CTCCCTCGAC ATGATCCACC TGGCGCACGC CCGCCAGGCT 
CAGCACTTAG CCACAGACAT AGGCTACAGA ACAGTGTCCC ATCACTTCAC AGCGTTGCCC 
ACGGACATGA AGGTGGAGTG GGCCAAGAAG GCGTATGGCT TGCAGAGTGA TAACCAATAC 
AGGGCAGATG TGAAGTGGAT GAAAGGCACG GGCTGGGTCG CCACCGGGTC ATTAAATGTG 
GAGCAGGCAA AGAAGGCAGG AGAACTCATT AGCGAGAAGA AGTACCGTCA GCATCCAGAT 
GCTTTGAAGT TTACCAGTAT TAAAGACACT CCGGAGATGG TCCAGGCCAG AATTAGTTAT 
ACCCAAGCAG TGGATAGGCT GTACAGGGAA CAAGGAGAGA ACATAAAGCA TCATTACACG 
CAGACCACAG ACCTGCCTGA GGTCCTGCTG GCCAAGCTGA ATGCCATGAA CATCAGCGAG 
ACACGGTATA AGGAATCCTG GAGTAAACTT CGAGACGGTG GATATAAACT AAGGTTGGAT 
GCCATTCCGT TCCAGGCAGC AAAGGCTTCT AGTGAAATCA TAAGTGATTA CAAATACAAA 
GAGGCATTTG AGAAAATGAA AGGACAGATG CTTGGCTCCC GGAGCTTGGA AGATGATCTC 
AGCCTTGCAC ATTCAGTGCA CGCGACCTCA CTGCAGAGTG ATGTGAATTA CAAGAAAGGC 
TTTGAACACT CAAAGGCACA CTTCCATCTG CCGTTGGACA TGGTAACCCT GGTGCACGCC 
AAGAAGGCTC AGACCCTGGC CAGCGACCAG GACTACAGAC ATCCGCTCCC CCAGCACACG 
TCCTTGGCAG AAGACCTGAG GCTGCGCTGT GCCAAGAAAG CTCACAAGTT GCAGAGCGAG 
AATTTGTACC GCTCAGACCT GAACTTCATG CGAGGCGTTG CCTGCGTCGT TCCAGGCACG 
TTGGAGATTG AAGGAAGAAA GAGGGCATCT GAGCTCATCA GTGAGAGTAA ATACCGTCAG 
CATCCTGGGT CGATCAAGTA CACGACTGTG ACGGACACCC CCAACCTCCT TCATGCAAAG 
TTCAGCAACC AGATTACCAA TGAGCGTCTC TATAAAGCAG CTGGAGAGGA TGCTAGACAC 
CAGTACACAA TGACCCTGGG TCTGCCCGAG TTTGTCCGAG CAAAAACAAA TGCGGCCAAT 
CTGAGCGACG CAAAATACAA GGAGTCTTGG CATGATCTCC GTGCTCAAGG CTACAAGCTG 
ACAATAGATG CGCTCCCCTT CCAGGCTGCA AGGGCCTCTG GGGACATCGC CAGCGATTTT 
CTCTACAGAC ACGACTTTGT GAAGGAGCGA GGGAAGCTCA TCGGGATCCG CAGCGTGAGC 
GATGACCCCC GGCTGCGGCA CTGTCGGCTC ATGGGCCAGC TGCAAAGTGA GCATCAGTAC 
AAGAGGGAGG CAGCCGGCAG CCAAGCCCAG TGCCACCTAC CCATGGACAT GGTGCACCTG 
GTCCATGCCA GGAAGGCCCA GGCCCTGGCC AGTGACCATG ACTACAGGAC TCAGTACCAC 
GAGTTCACGG CGCTACCCGA AGACTTGAAG TTGGCCTGGG CCAAGAAAGC CCACGCCCTG 
CAGAGTGAGT TTCGCTACAA GTCGGACCTC ATGGCCATGA AGGGGACAGG ATGGCTAGCA 
CTGAGATCCC CACAGATAGA GAGCGCCAGG AAGGCGGGAG AACTCATCAG CGAGACCAAA 
TACCGTAAAA AACCAGACAG TATCAAGTTC ACCACAGTGG TTGACTCCCC AGATCTGGTT 
CACGCCAAGA ATAGCTACGT GCATTGCAAC GAGCGCCTGT ACAGGTTGGG GGATGCAGAG 
GCCCTGCACA GATACACCCT GATCCCCGAC CACCCTGAGT TCACCCGAGC CCGCCTCAAC 
GCGCTGCACC TGAGTGACAA AGTCTACAGA AACTCCTGGG AGCAGACCCG CGCTGGCGGC 
TACGACTTCA GGCTGGACGC CATCCCGTTC CAGACTGCCC GGGCATCCAG GGAGATCGCC 
AGCGACTTCC GGTATAAAGA GGCTTTCCTG CGGGACCGGG GCCTGCAGAT CGGCTTCCGC 
AGCATCAACG ATGACCCGAG GATGAAGCAT TTCCTGAACG TCGGCAGGCT CCAGAGTGAC 
AATGAGTACA AGAAGGACTT TGCCAAGAGT CGGTCCCAGT TCCACAGCCG CACAGACCAG 
CCTGGCTTCC TCCAGGCCAA GAGGAGCCAG CAGTTGGCCA GCGACGTGCA TTACCGGCAG 
CCCCTGCCCC AGCCCACGTG CGACCCCGAG CAGCTGGGCC TCAAGCACGC GCAGAAGGCC 
CACCAGCTGC AGAGTGACGT CAAGTACAAA TCAGACTTGA ACCTGACCAG GGGTGTCGGC 
TGGACCCCTC CCGGCTCCTA CAAAGTGGAA ATGGCTCGGC GGGCTGCAGA ACTGGCCAAC 
ATGAGGGGCC TGGGGCTCCC GGGGGCTTAT GGGGAGCCGG AGGTGGTGGA GGCTGGAGGT 
CACCCGAGGG GGGAGGTGAA CCCAGATGCC ACTGAGATTC TGCACGTCAA AAGGAAAAAG 
GCTCTGTGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrap ( XM_021723330.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021579005.1 Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X5, mRNA.

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CloneID	OIc76026
Clone ID Related Accession (Same CDS sequence)	XM_021723330.1
Accession Version	XM_021723330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	nebulin-related-anchoring protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936486.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTTGCCTACA AGGCTGATTA CAAACACGAT ATTGTGGACT ACAACTATCC AGCCACCCTT 
ACTCCTGCCT ATCAAACAAC AATGAAACTG GTTCCTTTGA AAGATGTCAA CTACAGGCAA 
AGCATCGACA AGCTGAAGTA CAGCTCTGTG ACCAATACTC CGCAGATTGT TCAGGCCAAA 
ATCAATGCCC AGCAACTGAG CCACGTGAAC TACCGCGCTG ACTATGAGAA AAACAAGTTG 
AATTACACAC TGCCGCAGGA TGTGCCTCAG CTGGTGAAGG CCAGAACTAA CGCTGAACTC 
TTCAGTGAGG TTAAGTACAA AGAAGGCTGG GAGAAGACAA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CCATGTCTCT GTTGGCCGCC AAAGCCTCGG GGGAGCTTGC TAGCAATATT 
AAATACAAAG AAGAATATGA GAAAACAAAA GGCAAAGCCA TGGGAACAAC CGACTCGAAG 
CTTCTGCACT CCCTGCAGGT GGCCAAGATG AGCAGCGAGG TTGAATACAA GAAAGGCTTT 
GAGGAGAGTA AGACCCACTT TCACCTGCCC ATGGATATGG TAAACATCAG GCATGCTAAG 
AAGGCCCAAG CTCTTGCCAG TGACCTGGAT TACAGGAAGA AACTGCACGA ATACACCGTG 
CTGCCTGAAG ACATGAAGAC TCAGTGGGCC AAGAAAGCCT ACGGGCTCCA GAGCGAGCTG 
CAGTACAAAT CCGACCTGGC GTGGATGAAG GGAGTCGGGT GGCTGACTGA GGGCAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGACAA CTGGTCAGCG AGAAAAACTA CCGGCAGAGG 
GTTGATGAGC TGAAGTTCAC CAGTGTGACC GATAGTGCGC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGAGA ACCAGCAGTC TATCCACCAG 
TACACCATCA GCAAAGACGA GCCTCTCTTC CTGCAGGCCC GGGCCAATGC TGCTCACCTC 
AGCGAGAAAC GGTACAAGAG CAGCTGGGAA AACCAGAAGG CAAGAGGGTT TGAGCTGAGT 
CTTGATTCCT TGACCTTCCT GGCAGCCAAA GCCAAGAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAGG ACTATGAGAA GTCCAGAGGG AAGCTCATCG GGGCCAAGGG TGCACAGGGC 
GATTCTCAAA TGAGCCACTC GCTGCAAATG TCCAAACTGC AGAGCGAATT GGAGTACAAA 
AAGGGCTTCG AAGACACCAA GTCTCAGTGC CACGTCTCCC TCGACATGAT CCACCTGGCG 
CACGCCCGCC AGGCTCAGCA CTTAGCCACA GACATAGGCT ACAGAACAGT GTCCCATCAC 
TTCACAGCGT TGCCCACGGA CATGAAGGTG GAGTGGGCCA AGAAGGCGTA TGGCTTGCAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCAAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGCTGTACA GGGAACAAGG AGAGAACATA 
AAGCATCATT ACACGCAGAC CACAGACCTG CCTGAGGTCC TGCTGGCCAA GCTGAATGCC 
ATGAACATCA GCGAGACACG GTATAAGGAA TCCTGGAGTA AACTTCGAGA CGGTGGATAT 
AAACTAAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTAGTGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGAC AGATGCTTGG CTCCCGGAGC 
TTGGAAGATG ATCTCAGCCT TGCACATTCA GTGCACGCGA CCTCACTGCA GAGTGATGTG 
AATTACAAGA AAGGCTTTGA ACACTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ACGCCAAGAA GGCTCAGACC CTGGCCAGCG ACCAGGACTA CAGACATCCG 
CTCCCCCAGC ACACGTCCTT GGCAGAAGAC CTGAGGCTGC GCTGTGCCAA GAAAGCTCAC 
AAGTTGCAGA GCGAGAATTT GTACCGCTCA GACCTGAACT TCATGCGAGG CGTTGCCTGC 
GTCGTTCCAG GCACGTTGGA GATTGAAGGA AGAAAGAGGG CATCTGAGCT CATCAGTGAG 
AGTAAATACC GTCAGCATCC TGGGTCGATC AAGTACACGA CTGTGACGGA CACCCCCAAC 
CTCCTTCATG CAAAGTTCAG CAACCAGATT ACCAATGAGC GTCTCTATAA AGCAGCTGGA 
GAGGATGCTA GACACCAGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCAAAA 
ACAAATGCGG CCAATCTGAG CGACGCAAAA TACAAGGAGT CTTGGCATGA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGATGCGCTC CCCTTCCAGG CTGCAAGGGC CTCTGGGGAC 
ATCGCCAGCG ATTTTCTCTA CAGACACGAC TTTGTGAAGG AGCGAGGGAA GCTCATCGGG 
ATCCGCAGCG TGAGCGATGA CCCCCGGCTG CGGCACTGTC GGCTCATGGG CCAGCTGCAA 
AGTGAGCATC AGTACAAGAG GGAGGCAGCC GGCAGCCAAG CCCAGTGCCA CCTACCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TGGCCAGTGA CCATGACTAC 
AGGACTCAGT ACCACGAGTT CACGGCGCTA CCCGAAGACT TGAAGTTGGC CTGGGCCAAG 
AAAGCCCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCGG ACCTCATGGC CATGAAGGGG 
ACAGGATGGC TAGCACTGAG ATCCCCACAG ATAGAGAGCG CCAGGAAGGC GGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAAAAACCA GACAGTATCA AGTTCACCAC AGTGGTTGAC 
TCCCCAGATC TGGTTCACGC CAAGAATAGC TACGTGCATT GCAACGAGCG CCTGTACAGG 
TTGGGGGATG CAGAGGCCCT GCACAGATAC ACCCTGATCC CCGACCACCC TGAGTTCACC 
CGAGCCCGCC TCAACGCGCT GCACCTGAGT GACAAAGTCT ACAGAAACTC CTGGGAGCAG 
ACCCGCGCTG GCGGCTACGA CTTCAGGCTG GACGCCATCC CGTTCCAGAC TGCCCGGGCA 
TCCAGGGAGA TCGCCAGCGA CTTCCGGTAT AAAGAGGCTT TCCTGCGGGA CCGGGGCCTG 
CAGATCGGCT TCCGCAGCAT CAACGATGAC CCGAGGATGA AGCATTTCCT GAACGTCGGC 
AGGCTCCAGA GTGACAATGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAC 
AGCCGCACAG ACCAGCCTGG CTTCCTCCAG GCCAAGAGGA GCCAGCAGTT GGCCAGCGAC 
GTGCATTACC GGCAGCCCCT GCCCCAGCCC ACGTGCGACC CCGAGCAGCT GGGCCTCAAG 
CACGCGCAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGGGGTG TCGGCTGGAC CCCTCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCT 
GCAGAACTGG CCAACATGAG GGGCCTGGGG CTCCCGGGGG CTTATGGGGA GCCGGAGGTG 
GTGGAGGCTG GAGGTCACCC GAGGGGGGAG GTGAACCCAG ATGCCACTGA GATTCTGCAC 
GTCAAAAGGA AAAAGGCTCT GTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021579005.1
CDS	237..5186
Translation	MNVQACSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNAKKIPGAVGGIHDQEDGEQLRSVFHWDMKSKDGPDAPHRQPLVNERAYWPGYGESNAWCPGALPDPEIVRMVEARRSLGEAYPEDDEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKKGHEERISKFSTVADTPELLRAKAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMRGMAGPASGAEGQQGDLEEYEEPRGKGSFPAMITPAYQNAKKANELASDIKYRQDLHKMRGAAHYHSLPAQDNLVLKRAQSVNKLVSEVAYKADYKHDIVDYNYPATLTPAYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKARTNAELFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTTDSKLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVNIRHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKSDLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSAQMEHAKKSQELQSGVAYKAENQQSIHQYTISKDEPLFLQARANAAHLSEKRYKSSWENQKARGFELSLDSLTFLAAKAKRDLASEVKYKEDYEKSRGKLIGAKGAQGDSQMSHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMIHLAHARQAQHLATDIGYRTVSHHFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTTDLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASSEIISDYKYKEAFEKMKGQMLGSRSLEDDLSLAHSVHATSLQSDVNYKKGFEHSKAHFHLPLDMVTLVHAKKAQTLASDQDYRHPLPQHTSLAEDLRLRCAKKAHKLQSENLYRSDLNFMRGVACVVPGTLEIEGRKRASELISESKYRQHPGSIKYTTVTDTPNLLHAKFSNQITNERLYKAAGEDARHQYTMTLGLPEFVRAKTNAANLSDAKYKESWHDLRAQGYKLTIDALPFQAARASGDIASDFLYRHDFVKERGKLIGIRSVSDDPRLRHCRLMGQLQSEHQYKREAAGSQAQCHLPMDMVHLVHARKAQALASDHDYRTQYHEFTALPEDLKLAWAKKAHALQSEFRYKSDLMAMKGTGWLALRSPQIESARKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYVHCNERLYRLGDAEALHRYTLIPDHPEFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGFRSINDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSRTDQPGFLQAKRSQQLASDVHYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELANMRGLGLPGAYGEPEVVEAGGHPRGEVNPDATEILHVKRKKALWM

Target ORF information:

RefSeq Version	XM_021723330.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus nebulin related anchoring protein (Nrap), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021723330.1

ORF Insert Sequence:

ATGAACGTGC AGGCCTGCTC CAGGTGTGGC TATGGGGTGT ATCCTGCTGA GAAGATCAGC 
TGCATTGACC AGATCTGGCA TAAAGCATGT TTCCACTGTG AAGTGTGCAA GATGATGCTG 
TCTGTGAATA ACTTCGTGAG TCACCAGAAA AAGCCCTACT GTCACGCCCA TAACCCCAAG 
AACAACACGT TCACTAGTGT CTATCACACG CCATTAAATC TAAATGCGAA GAAGATTCCA 
GGAGCCGTGG GTGGGATCCA TGACCAAGAA GATGGTGAAC AGTTGAGATC GGTTTTCCAC 
TGGGACATGA AATCCAAGGA TGGACCCGAT GCACCTCACA GGCAACCGCT GGTGAACGAG 
AGAGCCTACT GGCCTGGATA TGGGGAAAGC AATGCTTGGT GCCCAGGAGC TCTGCCAGAC 
CCGGAAATCG TAAGGATGGT TGAGGCCCGA CGGTCTCTTG GTGAGGCGTA TCCAGAAGAC 
GATGAGCAGC AGAGAGGCAA GGGGAGCTTT CCGGCCATGA TCACACCTGC TTATCAGAGG 
GCCAAGAAAG CCAACCAGCT GGCCAGCCAG GTGGAATACA AGAAAGGGCA TGAGGAGCGC 
ATCTCCAAGT TCTCCACGGT GGCGGATACT CCTGAGCTGC TGCGGGCCAA GGCAGGGGGA 
CAGCTACAGA GTGATGTGAG ATACACGGAG GACTATGAGC AGCAGAGAGG GAAGGGCAGT 
TTTCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAAA GGGCCAACGA GCTGGCGAGT 
GACGTGAGAT ACCATCAACA ATACCAAAGA GAAATGAGAG GAATGGCGGG TCCAGCCTCT 
GGAGCTGAGG GCCAGCAGGG TGACTTGGAG GAGTATGAGG AGCCCAGGGG AAAGGGCAGC 
TTCCCCGCCA TGATCACCCC AGCTTATCAG AATGCCAAGA AGGCCAACGA ACTTGCAAGT 
GATATAAAAT ACAGGCAGGA CCTGCACAAG ATGAGGGGCG CCGCGCATTA TCATTCTCTT 
CCAGCCCAAG ACAACCTGGT TCTCAAACGG GCCCAGAGTG TGAACAAGCT CGTGAGCGAG 
GTTGCCTACA AGGCTGATTA CAAACACGAT ATTGTGGACT ACAACTATCC AGCCACCCTT 
ACTCCTGCCT ATCAAACAAC AATGAAACTG GTTCCTTTGA AAGATGTCAA CTACAGGCAA 
AGCATCGACA AGCTGAAGTA CAGCTCTGTG ACCAATACTC CGCAGATTGT TCAGGCCAAA 
ATCAATGCCC AGCAACTGAG CCACGTGAAC TACCGCGCTG ACTATGAGAA AAACAAGTTG 
AATTACACAC TGCCGCAGGA TGTGCCTCAG CTGGTGAAGG CCAGAACTAA CGCTGAACTC 
TTCAGTGAGG TTAAGTACAA AGAAGGCTGG GAGAAGACAA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CCATGTCTCT GTTGGCCGCC AAAGCCTCGG GGGAGCTTGC TAGCAATATT 
AAATACAAAG AAGAATATGA GAAAACAAAA GGCAAAGCCA TGGGAACAAC CGACTCGAAG 
CTTCTGCACT CCCTGCAGGT GGCCAAGATG AGCAGCGAGG TTGAATACAA GAAAGGCTTT 
GAGGAGAGTA AGACCCACTT TCACCTGCCC ATGGATATGG TAAACATCAG GCATGCTAAG 
AAGGCCCAAG CTCTTGCCAG TGACCTGGAT TACAGGAAGA AACTGCACGA ATACACCGTG 
CTGCCTGAAG ACATGAAGAC TCAGTGGGCC AAGAAAGCCT ACGGGCTCCA GAGCGAGCTG 
CAGTACAAAT CCGACCTGGC GTGGATGAAG GGAGTCGGGT GGCTGACTGA GGGCAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGACAA CTGGTCAGCG AGAAAAACTA CCGGCAGAGG 
GTTGATGAGC TGAAGTTCAC CAGTGTGACC GATAGTGCGC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGAGA ACCAGCAGTC TATCCACCAG 
TACACCATCA GCAAAGACGA GCCTCTCTTC CTGCAGGCCC GGGCCAATGC TGCTCACCTC 
AGCGAGAAAC GGTACAAGAG CAGCTGGGAA AACCAGAAGG CAAGAGGGTT TGAGCTGAGT 
CTTGATTCCT TGACCTTCCT GGCAGCCAAA GCCAAGAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAGG ACTATGAGAA GTCCAGAGGG AAGCTCATCG GGGCCAAGGG TGCACAGGGC 
GATTCTCAAA TGAGCCACTC GCTGCAAATG TCCAAACTGC AGAGCGAATT GGAGTACAAA 
AAGGGCTTCG AAGACACCAA GTCTCAGTGC CACGTCTCCC TCGACATGAT CCACCTGGCG 
CACGCCCGCC AGGCTCAGCA CTTAGCCACA GACATAGGCT ACAGAACAGT GTCCCATCAC 
TTCACAGCGT TGCCCACGGA CATGAAGGTG GAGTGGGCCA AGAAGGCGTA TGGCTTGCAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCAAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGCTGTACA GGGAACAAGG AGAGAACATA 
AAGCATCATT ACACGCAGAC CACAGACCTG CCTGAGGTCC TGCTGGCCAA GCTGAATGCC 
ATGAACATCA GCGAGACACG GTATAAGGAA TCCTGGAGTA AACTTCGAGA CGGTGGATAT 
AAACTAAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTAGTGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGAC AGATGCTTGG CTCCCGGAGC 
TTGGAAGATG ATCTCAGCCT TGCACATTCA GTGCACGCGA CCTCACTGCA GAGTGATGTG 
AATTACAAGA AAGGCTTTGA ACACTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ACGCCAAGAA GGCTCAGACC CTGGCCAGCG ACCAGGACTA CAGACATCCG 
CTCCCCCAGC ACACGTCCTT GGCAGAAGAC CTGAGGCTGC GCTGTGCCAA GAAAGCTCAC 
AAGTTGCAGA GCGAGAATTT GTACCGCTCA GACCTGAACT TCATGCGAGG CGTTGCCTGC 
GTCGTTCCAG GCACGTTGGA GATTGAAGGA AGAAAGAGGG CATCTGAGCT CATCAGTGAG 
AGTAAATACC GTCAGCATCC TGGGTCGATC AAGTACACGA CTGTGACGGA CACCCCCAAC 
CTCCTTCATG CAAAGTTCAG CAACCAGATT ACCAATGAGC GTCTCTATAA AGCAGCTGGA 
GAGGATGCTA GACACCAGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCAAAA 
ACAAATGCGG CCAATCTGAG CGACGCAAAA TACAAGGAGT CTTGGCATGA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGATGCGCTC CCCTTCCAGG CTGCAAGGGC CTCTGGGGAC 
ATCGCCAGCG ATTTTCTCTA CAGACACGAC TTTGTGAAGG AGCGAGGGAA GCTCATCGGG 
ATCCGCAGCG TGAGCGATGA CCCCCGGCTG CGGCACTGTC GGCTCATGGG CCAGCTGCAA 
AGTGAGCATC AGTACAAGAG GGAGGCAGCC GGCAGCCAAG CCCAGTGCCA CCTACCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TGGCCAGTGA CCATGACTAC 
AGGACTCAGT ACCACGAGTT CACGGCGCTA CCCGAAGACT TGAAGTTGGC CTGGGCCAAG 
AAAGCCCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCGG ACCTCATGGC CATGAAGGGG 
ACAGGATGGC TAGCACTGAG ATCCCCACAG ATAGAGAGCG CCAGGAAGGC GGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAAAAACCA GACAGTATCA AGTTCACCAC AGTGGTTGAC 
TCCCCAGATC TGGTTCACGC CAAGAATAGC TACGTGCATT GCAACGAGCG CCTGTACAGG 
TTGGGGGATG CAGAGGCCCT GCACAGATAC ACCCTGATCC CCGACCACCC TGAGTTCACC 
CGAGCCCGCC TCAACGCGCT GCACCTGAGT GACAAAGTCT ACAGAAACTC CTGGGAGCAG 
ACCCGCGCTG GCGGCTACGA CTTCAGGCTG GACGCCATCC CGTTCCAGAC TGCCCGGGCA 
TCCAGGGAGA TCGCCAGCGA CTTCCGGTAT AAAGAGGCTT TCCTGCGGGA CCGGGGCCTG 
CAGATCGGCT TCCGCAGCAT CAACGATGAC CCGAGGATGA AGCATTTCCT GAACGTCGGC 
AGGCTCCAGA GTGACAATGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAC 
AGCCGCACAG ACCAGCCTGG CTTCCTCCAG GCCAAGAGGA GCCAGCAGTT GGCCAGCGAC 
GTGCATTACC GGCAGCCCCT GCCCCAGCCC ACGTGCGACC CCGAGCAGCT GGGCCTCAAG 
CACGCGCAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGGGGTG TCGGCTGGAC CCCTCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCT 
GCAGAACTGG CCAACATGAG GGGCCTGGGG CTCCCGGGGG CTTATGGGGA GCCGGAGGTG 
GTGGAGGCTG GAGGTCACCC GAGGGGGGAG GTGAACCCAG ATGCCACTGA GATTCTGCAC 
GTCAAAAGGA AAAAGGCTCT GTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nrap
Entrez Gene ID	101967358
Full Name	nebulin related anchoring protein
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nrap cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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