N4bp2 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	N4bp2
Entrez Gene ID	101961497
Full Name	NEDD4 binding protein 2
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021722741.1	XP_021578416.1	NEDD4-binding protein 2 isoform X2
XM_013356276.2	XP_013211730.1	NEDD4-binding protein 2 isoform X1
XM_021722740.1	XP_021578415.1	NEDD4-binding protein 2 isoform X2
XM_013356276.1	XP_013211730.1	NEDD4-binding protein 2
XM_021722737.1	XP_021578412.1	NEDD4-binding protein 2 isoform X1
XM_021722738.1	XP_021578413.1	NEDD4-binding protein 2 isoform X1
XM_021722742.1	XP_021578417.1	NEDD4-binding protein 2 isoform X2
XM_021722743.1	XP_021578418.1	NEDD4-binding protein 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following N4bp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N4bp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc51276	XM_021722737.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc51276	XM_021722738.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc51276	XM_013356276.2 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc75676	XM_021722740.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc75676	XM_021722741.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc75676	XM_021722742.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc75676	XM_021722743.1 Latest version!	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OIc51276	XM_013356276.1	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N4bp2 ( XM_013356276.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013211730.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIc51276
Clone ID Related Accession (Same CDS sequence)	XM_013356276.2 , XM_013356276.1 , XM_021722737.1 , XM_021722738.1
Accession Version	XM_013356276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013356276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013211730.1
CDS	23..5344
Translation	MPRKRKNLGGNPIRKTANSKEVVVSSLASHEESTTTLPSVCETNTDQEELFTNISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_013356276.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356276.2

ORF Insert Sequence:

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_013356276.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013211730.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), mRNA.

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CloneID	OIc51276
Clone ID Related Accession (Same CDS sequence)	XM_013356276.2 , XM_013356276.1 , XM_021722737.1 , XM_021722738.1
Accession Version	XM_013356276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013211730.1
CDS	119..5440
Translation	MPRKRKNLGGNPIRKTANSKEVVVSSLASHEESTTTLPSVCETNTDQEELFTNISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_013356276.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013356276.1

ORF Insert Sequence:

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722737.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578412.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc51276
Clone ID Related Accession (Same CDS sequence)	XM_013356276.2 , XM_013356276.1 , XM_021722737.1 , XM_021722738.1
Accession Version	XM_021722737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578412.1
CDS	313..5634
Translation	MPRKRKNLGGNPIRKTANSKEVVVSSLASHEESTTTLPSVCETNTDQEELFTNISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722737.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722737.1

ORF Insert Sequence:

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722738.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578413.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X2, mRNA.

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CloneID	OIc51276
Clone ID Related Accession (Same CDS sequence)	XM_013356276.2 , XM_013356276.1 , XM_021722737.1 , XM_021722738.1
Accession Version	XM_021722738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578413.1
CDS	222..5543
Translation	MPRKRKNLGGNPIRKTANSKEVVVSSLASHEESTTTLPSVCETNTDQEELFTNISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722738.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722738.1

ORF Insert Sequence:

ATGCCAAGGA AAAGGAAAAA TCTTGGGGGA AATCCTATTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTCT TGCTAGTCAT GAGGAGTCAA CCACTACCCT TCCTTCCGTG 
TGTGAGACAA ACACTGATCA GGAAGAACTC TTCACCAATA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTATA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCC 
ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722741.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578416.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X5, mRNA.

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CloneID	OIc75676
Clone ID Related Accession (Same CDS sequence)	XM_021722741.1 , XM_021722740.1 , XM_021722742.1 , XM_021722743.1
Accession Version	XM_021722741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578416.1
CDS	179..5260
Translation	MDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722741.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722741.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722740.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578415.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X4, mRNA.

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CloneID	OIc75676
Clone ID Related Accession (Same CDS sequence)	XM_021722741.1 , XM_021722740.1 , XM_021722742.1 , XM_021722743.1
Accession Version	XM_021722740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578415.1
CDS	298..5379
Translation	MDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722740.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722740.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722742.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578417.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X6, mRNA.

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CloneID	OIc75676
Clone ID Related Accession (Same CDS sequence)	XM_021722741.1 , XM_021722740.1 , XM_021722742.1 , XM_021722743.1
Accession Version	XM_021722742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578417.1
CDS	206..5287
Translation	MDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722742.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722742.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4bp2 ( XM_021722743.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021578418.1 Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X7, mRNA.

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CloneID	OIc75676
Clone ID Related Accession (Same CDS sequence)	XM_021722741.1 , XM_021722740.1 , XM_021722742.1 , XM_021722743.1
Accession Version	XM_021722743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936482.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021578418.1
CDS	214..5295
Translation	MDCLLELSASDAKIEESSSQSFVASENQPVETGSEIMEKCPLEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVNSFNDPSKFINSHSSNTAPVHCMQNMDSNSENLENSVSTVSSNPLTSHSVLNGSKSFIKDNTLALEDDYQEDSHLSNSLNPTNDSIVGCSNLNQRQSELLESEHVEAQLSEAPVNLNANEPQASLNLPVQNSGCDLPDTGGNQRSTSVSDIFVPSEGFNFKPHKHTELPPKGKDMNYCPVLTPLPLLLPPPPPPPMWNPMIPAFDLFQGSHGFVAPVVTTAAHWRPVNYTFPPSIISHTSPTKVWRNQEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKISPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGISKEKITRMLDHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSSSPRDNEDITSEKEENVLSSSLKHLELIEEKNPEVTKETVLPENITYLPHADISKGRKEISGTHHNTQSQFIQEAAHIYLSDSGSKVQVADKREKKEQVEMTTEKQCIKTDADRVSPSICSGENNQDCDLANTVPLQNEKSSSGEILEERTTVKKKAFGKQKSKSPSEKFPRHELSNFVGDWPVEKTIGQRTKRNRKSEKASSIQSDKKYSRPQSRKVSDNSVSMITDHIQQQGSAYESVDDGRKSQCDDSSEFLNSCKYDTYKNPEKNSFNIVGDWPSSDSLAQREHRSKMPKADLSEPNLEFGTNDNMNEISLYTTQSCWGTCPKELKFLGSSTLQSADMIPSEMTQENQTYLSKMIHGQHTLPLTFTSNTATITGVVGPQILAEFPGEMTRGVPGIEVGTCTQTEPQDFALLWKIEKNKISVSDSIKVLIGRLDGFKPKAFNINTKSNVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTELDNFQKSYDESQVGPFSMGLNLKEIISQRETVEDSNSFVPEISPGICISNINSQSSYNAEKLNSEQAEIRAVTPEKLGSITNVFPNAAVDLKSNSEILRNSQVELSGLCSFKQSLPVILKSATPKDVSEIENSLVAAETEDGMHSSLNLSDILNSVSSTSNLELNEEVYFTDSLEIKKNENFPKDYVKFVNSEEFMNVDEQEMEKILMAGSSLSVGVNEEDKTEILNPMPGMAKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKVIQDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAAPEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQDFVHQNENVTSHTAQKTKEKKAKKLKETEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRMECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEHLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_021722743.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus NEDD4 binding protein 2 (N4bp2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722743.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAATT GTCTGCCTCT GATGCCAAGA TAGAAGAATC ATCTTCACAA 
AGCTTTGTTG CTTCTGAGAA CCAGCCAGTT GAAACAGGAA GTGAAATAAT GGAGAAGTGT 
CCTCTTGAAG AAGAGAGTGA AGATTCGAAG ATGGATTCAT TTCTGGATAT GCAGCTGACC 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAATAGTT TTAATGACCC AAGTAAATTT 
ATAAATTCAC ATTCAAGTAA TACGGCTCCT GTCCATTGTA TGCAAAATAT GGATTCCAAC 
AGTGAGAATT TAGAAAATTC TGTCTCTACA GTAAGTTCAA ACCCATTAAC TTCACATTCA 
GTTTTGAATG GGTCCAAAAG TTTTATAAAG GATAACACTT TGGCTTTAGA AGATGATTAC 
CAAGAAGATT CTCATCTCAG TAATTCTTTA AATCCAACAA ATGACTCTAT CGTGGGTTGT 
AGCAATCTTA ATCAGAGACA GAGCGAGCTT TTAGAATCTG AGCATGTTGA GGCTCAACTT 
TCCGAAGCCC CTGTAAATTT GAATGCTAAC GAACCTCAAG CTTCTTTAAA CCTTCCTGTG 
CAAAATTCAG GGTGTGATTT GCCAGATACT GGTGGGAATC AGAGGTCTAC TTCTGTCTCT 
GATATTTTTG TACCTTCTGA AGGGTTCAAT TTCAAGCCAC ACAAACATAC TGAACTACCA 
CCAAAGGGGA AGGATATGAA TTACTGCCCA GTACTTACCC CTCTCCCTTT ACTGCTTCCT 
CCTCCTCCAC CTCCACCAAT GTGGAATCCA ATGATTCCTG CTTTTGACCT CTTTCAAGGA 
AGCCATGGGT TTGTGGCTCC TGTTGTAACC ACAGCTGCAC ACTGGAGACC CGTCAACTAC 
ACATTTCCAC CCTCGATCAT TTCTCACACT TCCCCAACGA AGGTATGGAG AAACCAAGAG 
GGAACAAGTG CTTATCAAGT ACAAGAAACT CCTGTTTCTC AAGTTGTAAG AAAAAAGACA 
ACATCATATG TTGGACTAGT TCTTGTTCTT CTCAGAGGCC TTCCAGGATC AGGAAAATCT 
TTTTTGGCGA GGACTTTGCA AGAGGATAAT CCAAGTGGAG TCATTCTTAG TACTGATGAT 
TATTTTTATA TAAATGGACA GTACCAGTTC GATGTAAAGT ACTTAGGAGA AGCACATGAA 
TGGAACCAGA ATCGTGCTAA AGAAGCATTT GAGAAGAAGA TATCACCAAT AATAATAGAC 
AATACAAACC TACAGGCCTG GGAAATGAAG CCATATGTTG CTTTGTCTCA AAAACATAAA 
TATAAGGTCC TCTTCCGGGA ACCGGACACA TGGTGGAAAT TCAAACCGAA GGAGCTTGCA 
AGGCGTAATA TTCATGGGAT AAGCAAAGAA AAAATAACAA GAATGTTAGA CCACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATTATGAGT TCTTCAGTTC CAGAGAAAAT CGAACGTATT 
GAATTATGTG CATATTCTTG TGAGGATAGA AGTTCCAGCC CAAGAGACAA TGAAGATATT 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCACTGA AGCATCTAGA ATTAATTGAA 
GAAAAGAATC CTGAAGTGAC CAAAGAAACT GTGTTGCCTG AGAATATTAC ATATCTCCCC 
CATGCAGATA TAAGCAAAGG AAGGAAAGAA ATAAGTGGTA CACATCATAA CACTCAGAGT 
CAGTTCATTC AGGAAGCTGC ACACATCTAT CTTTCAGACT CTGGAAGCAA AGTTCAAGTA 
GCAGACAAGA GAGAAAAAAA AGAGCAAGTA GAAATGACAA CTGAAAAACA GTGTATTAAA 
ACTGATGCAG ATAGAGTATC ACCAAGTATT TGCTCTGGTG AAAATAATCA AGACTGCGAT 
CTGGCAAATA CTGTACCACT TCAAAATGAA AAATCTTCAT CAGGTGAAAT ATTAGAAGAA 
AGAACAACAG TAAAGAAAAA AGCCTTTGGG AAACAAAAAA GCAAATCACC TTCGGAAAAG 
TTTCCAAGAC ATGAGCTATC GAATTTTGTT GGTGATTGGC CAGTTGAGAA GACTATTGGT 
CAGAGAACAA AAAGGAACCG AAAATCTGAA AAAGCTTCAT CTATACAAAG CGACAAAAAG 
TATAGTCGCC CTCAGTCACG CAAAGTATCA GATAATAGTG TGTCCATGAT TACAGATCAT 
ATCCAACAAC AAGGATCTGC ATATGAAAGT GTAGATGATG GCAGGAAGTC ACAGTGTGAT 
GATTCTTCAG AATTTCTCAA TAGCTGTAAA TATGATACTT ATAAAAATCC TGAAAAAAAC 
TCATTTAATA TTGTAGGTGA CTGGCCTTCA TCTGATTCTT TAGCTCAGAG AGAGCATAGA 
TCAAAAATGC CAAAGGCTGA TTTAAGTGAA CCCAACCTAG AATTTGGAAC TAATGATAAT 
ATGAATGAAA TATCCTTATA TACAACACAA TCCTGTTGGG GCACATGCCC TAAAGAACTA 
AAATTCTTGG GGAGCTCCAC TCTACAAAGT GCTGACATGA TACCCAGTGA AATGACCCAG 
GAGAATCAAA CTTATCTAAG TAAAATGATT CATGGGCAGC ACACATTGCC TCTAACTTTC 
ACGAGTAATA CAGCAACCAT TACTGGAGTG GTAGGGCCAC AAATTCTAGC TGAATTTCCA 
GGGGAAATGA CTAGAGGAGT CCCCGGAATA GAAGTAGGCA CATGTACCCA GACTGAACCA 
CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TCAGTGTTTC AGATTCTATC 
AAAGTATTAA TAGGAAGATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG 
TCAAATGTTC AAGAGACAAT TCCATATAGG GTAATGTATG ATAAAAGCAC GTATGTTGAA 
GAAAGTGAAC TTACCAGTGC TGATGAATCT GAAAATCTTA ACATCCTTTG TAAGCTGTTT 
GGATCTTTTT CATTAGAAGC CTTAAAAGAT TTATATGAAA GGTGTAATAA AGATATTATT 
TGGGCCACAA GTCTTCTGTT GGATTCTGAA ACTAAATTAT GTGAGGATAC TGAATTAGAC 
AATTTCCAAA AATCATATGA TGAATCACAA GTTGGACCAT TTTCTATGGG ATTGAATTTG 
AAGGAAATTA TTAGCCAAAG AGAAACAGTA GAGGATTCTA ATTCTTTTGT GCCAGAAATT 
AGTCCTGGAA TCTGTATCAG TAATATTAAT TCACAATCTA GCTATAATGC TGAAAAGTTA 
AACTCAGAGC AGGCAGAAAT AAGAGCCGTA ACTCCTGAAA AGCTTGGATC AATAACAAAT 
GTATTTCCTA ATGCTGCTGT AGATCTGAAG AGTAATAGTG AAATACTTCG AAACAGCCAG 
GTAGAACTAT CAGGTTTATG TAGCTTCAAG CAGTCTCTTC CAGTTATTCT AAAATCCGCT 
ACCCCTAAAG ATGTGAGTGA AATAGAGAAC AGTCTGGTAG CGGCAGAGAC TGAAGATGGT 
ATGCATTCTT CTTTAAATTT ATCAGATATT TTAAACTCTG TATCAAGTAC TTCAAATCTT 
GAATTAAATG AAGAAGTTTA CTTTACTGAT TCTTTGGAAA TAAAGAAAAA TGAAAATTTT 
CCAAAAGATT ATGTGAAATT TGTGAACTCT GAAGAATTTA TGAATGTAGA TGAACAGGAA 
ATGGAGAAAA TTCTAATGGC GGGAAGTAGC TTGTCAGTTG GAGTTAATGA AGAAGATAAA 
ACTGAGATAT TGAATCCCAT GCCAGGGATG GCCAAATCTC TGACCATAGA CTGTCTGGAA 
TTGGCATTAC CCCCAGAACT GGCTTTTCAA CTTAATGAAT TATTTGGCCC AGTTGGTATT 
GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA 
GTGATTCATG AGAAATGGAA AGAATCTGTA ATGGAGCGAC AAAGACAAGA GGAAGTATCT 
TGTGGCAAGG TTATTCAAGA TTCTTCCCTG GTTGGACATA TTGGTCTTGA TAATCCTGAA 
CAAAAATCAT CTCAGAGAAC AGGCAAAAAA TTGCTGAAGA CTTTAGCAGC ACCCGAAATG 
CTCCCCTTGT TGGATCATTG GAATACTCAA ACTAAAAAAG TATCACTCAG AGAAATAATG 
TCGGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGACACT TATGTTTGAA 
AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC 
CAAAATTTTT TGGTGGACAT TTTCAAGGAT CACAACTATT CGTTAGAACA CACAGTGCAG 
TTTTTAAACT GTGTTCTCGA AGGAGATCCT GTAAAAACAG TTGTAGCTCA AGACTTTGTT 
CACCAAAATG AGAATGTCAC TTCTCATACT GCACAGAAGA CTAAAGAGAA AAAGGCAAAG 
AAATTGAAGG AGACTGAAGA TACCCCAAGT GAACCATCTT TCCAGGATTT CGAGTACCCT 
GAGTATGATG ACTACAGGGC AGAGGCTTTC CTGCACCAGC AGAAGAGGAT GGAATGCTAT 
AGCAAAGCTA AGGAAGCTTA CCGAATGGGG AAGAAAAATG TTGCCACCTT TTATGCCCAG 
CAGGGCAGTC TTCATGAGCA GAAAATGAAA GAAGCCAACC ACCTTGCTGC TGTGGAGATC 
TTTGAGAAAG TTAATGCCTC CCTGCTGCCA CAGAATGTCT TAGACCTCCA TGGGCTGCAT 
GTGGATGAAG CAATAGAACA TTTGATGACA GTTTTACAGC AGAAAACTGA AGAGTTTAAA 
CAGAATGGTG GTAAGCCCTA TCTTTCTGTG ATTACTGGGA GAGGAAACCA CAGCCAGGGA 
GGAGTTGCTC GCATCAAACC AGCTGTCATT AAATACCTCA CAAGCCATAG CTTCAGGTTC 
TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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N4bp2 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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