Med12 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med12
Entrez Gene ID	101961401
Full Name	mediator complex subunit 12
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005339966.2	XP_005340023.1	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12
XM_005339966.3	XP_005340023.1	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12

Pathways from BioSystems
Gene Ontology

The following Med12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc70762	XM_005339966.3 Latest version!	Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
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OIc70762	XM_005339966.2	Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med12 ( XM_005339966.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005340023.1 Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.

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CloneID	OIc70762
Clone ID Related Accession (Same CDS sequence)	XM_005339966.2 , XM_005339966.3
Accession Version	XM_005339966.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936762.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005339966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGCCTGGGG 
CCTCCGGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTATCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTACC TGACACTGGT CGCAGGAAGC CTCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGG GCGATCCCAG AATGCCATTA ACACCTGGTT CACTGATCTG 
GCTGGCACTA AACCGCTCAC ACAATTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GATACTTAGC CAAATACACA GTGCCTGTGA TGAGGGCCGC CTGGCTCATT 
AAGATGACCT CTGCCTACTA TGCAGCAATC ACTGAAACCA AGGTTAAGAA GAGACATGTT 
GACCCCTTCA TTGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GCTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC TACAATAGGG 
CCCTTGCCCC ATGATGTGGA GGTGGCAATC AGGCAGTGGG ACTACAATGA GAAGCTAGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGATT TCCTGACCTG GGTTCTAGAA 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGTTGCT GCCCCTACTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTGT CCCGCCGCCT TGCCTACTTT 
TGTACCCGGA GACTGGCCCT GCAACTGGAT GGCATGAGTA GCCACTCATC TCATGTTATA 
TCTGCTCAGT CGACAAGCAC ACTGCCTACC ACTCCTGCCC CTCAGCCCCC AACTAGCAGC 
ACACCCTCCA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGTCTCAGCT GTATACTACA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAT 
TATTCACTGA CTGATAGCCG AATTAAGACG GGTTCACCAC TTGACCACCT GCCTATTGCC 
CCTTCCAACC TGCCCATGCC AGAAGGCAAC AGTGCCTTCA CTCAGCAAGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC CACTGCAGGC TTTACCATTG GACGGGTGCT CCATACTTTG 
GAAGTTCTGG ACAGTCATAG TTTTGAGCGC TCCGACTTCA GCAACTCTCT TGACTCTCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC TTCTGATGAT 
GATGCTGTGG TTTCATTATT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGTCAT 
CGTGCCATGG TGGTAGCCAA GCTTCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CCGAAGCAGC AGATGAGAAG GGTTCCATTG CTTCTGGTTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGATC CCCGAAGTGA GAGCGAGCGG GTGGAATTCT TTAACTTGGT ACTGCTTTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTAATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCGCGGCCTC CCTCTCCCTT TGATGATCCT 
GTCGATGACC CAGAGCGCAA GGAGGCTGAA GGTAGCAGCA GTAGCAAGCT GGAGGATCCA 
GCACTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGTTGTTTGA GGATATGGAG 
AAGCCTGACT TCTCATTGTT TTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGCCCATCT 
CCTGAGAAAC CAGATGTAGA GAAGGAGGTG AAGCCACCAC CCAAGGAGAA GATTGAAGGA 
ACCCTTGGGG TTCTTTATGA CCAGCCACGA CATGTGCAGT ACGCCACACA CTTTCCTATT 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTTGTACT ATTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGCCG CAACCGACCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AACATCTTTC ACATTATGAC 
CAACACCAGG TCACTGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTGGTTG AAGCCGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTACC ATGCCTGTCT CATCCTGAAC 
CAGGACCAGA TGGCGCAGGT CTTTGAGGGG CTGTGTGGTG TAGTGAAGCA TGGGATGAAC 
CGATCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAATACCA TCTACTGTAA TGTGGAGCCA TCAGAATCTA ATATGCGCTG GGCACCCGAG 
TTTATGATCG ACACCCTGGA GAACCCTGCT GCTCATACCT TCACCTACAC AGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTAG GGCACCATGA TCCCGATAGG GTGAATGACA TTGCAATCCT GTGTGCAGAG 
CTGACCGGCT ACTGCAAGTC ACTAAGTGCT GAGTGGCTAG GAGTACTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGCGGTTTC AATGATCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCCT TTCATGACTC CTTGGCTACT TTTGTTGCCA TCCTCATTGC TCGCCAGTGT 
TTGCTCCTAG AAGATCTGAT CCGCTGTGCT GCCATCCCGT CACTCCTGAA TGCTGCTTGT 
AGTGAGCAGG ACTCAGAGCC TGGGGCCCGG CTTACTTGCC GCATCCTTCT CCACCTTTTC 
AAGACACCAC AACTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT CGGAATCCGC 
TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAAGAGGAGG GAGGAGGTGG CAGTGGCGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCGCTA 
TGTGAGGACA GCAATGACCT ACAGGACCCA GTGTTGAGTA GCGCCCAGGC CCAGCGCCTC 
ATGCAGCTCA TCTGCTACCC ACATCGACTT CTGGACAATG AGGATGGGGA AAACCCCCAG 
AGGCAGCGCA TTAAGCGTAT TCTCCAGAAT TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAAGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCA 
GGAAGCACTG CGAACAACAT GCCCAGCAGC AGCAAGACCA AACCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCCA AGCTGCCCAC CTCAGTCCAA 
GGGCATGTAT TAAAGGCTGC TGGAGAGGAA CTGGAGAAGG GTCAGCATCT GGGCTCTTCT 
TCTCGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTGTTGAG CCAGCAGCCC 
TTCTTATCCC TGGTGCTGAC ATGTCTGAAA GGGCAAGATG AGCAACGTGA GGGACTCCTT 
ACCTCCCTCT ACAACCAAGT GAACCAGATT GTGAATAATT GGCGAGAGGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACAGT GCAACGCAGC ACCCAGCAGA CCACAGAGTG GGCCATGCTC 
CTCCTGGAAA TCATCATCAG TGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTTACT 
ACTGTGTTGG ACATGCTGAG TGTACTCATC AATGGAACCT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GCAGCATGGA GGAAAACAAA CGTGCATATA TGAACCTAGT GAAGAAGCTG 
CAGAAGGAAT TGGGAGAGCA TCAATCAGAC AGTCTGGAAA AGGTTCGCCA GTTGCTACCA 
CTGCCCAAGC AGACTAGAGA TGTCATCACG TGTGAACCAC AGGGCTCCCT CATTGACACC 
AAAGGCAACA AGATTGCTGG CTTTGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACAAAACAGA AGATATCTCC CTGGGATCTC TTCGAGGGCT TGAAGCTCTC TGCACCACTC 
TCCTGGGGCT GGTTTGGGAC AGTCCGAGTT GACCGGCGAG TGGCTCGAGG GGAGGAGCAG 
CAGCGCTTGC TACTCTACCA CACACACCTG AGGCCCCGGC CCCGTGCCTA TTACCTAGAG 
CCACTGCCAC TGCCTCCAGA AGATGAGGAG CCACCAGCTC CCACCCTGCT AGAACCTGAG 
AAAAAGGCTC CTGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCCAC CAAGACAGAG 
GACTATGGCA TGGGCCCAGG CCGAAGTGGT CCCTATGGTG TGACAGTGCC TCCAGACCTC 
CTGCACCACC CCAACCCTGG ATCCATATCT CACCTGAGCT ACAGGCAGGG CCCCATAGGC 
CTGTATACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATATCGCCCT 
GTGAGGTTAC CAATGCAGAA GCTGCCAACC CGACCAGCTT ACCCTGGAGT GCTACCCACA 
ACTGTGACTG GTGTTATGGG CCTAGAACCT TCATCTTACA AGACTTCCGT GTACCGACAG 
CAGCAACCTG CAGTGCCCCA GGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGGA TGTTGGGACA GTCGTCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGC 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTATCA GAGCACCCAC 
CCCTCTACCA ATCCTACTCT TGTAGATCCT ACCCGTCACC TGCAACAGCG GCCCAGTGGC 
TATGTACACC AGCAGGCCCC AACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC CACCATGCTA GGTACCATGA CTCCACTGAA TACCCAGGGC 
GTCCAGGCAG GTNNCCGCTC AACTTCCATC CTACCTGAGC AGCAGCAGCA GCAACAACAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGTACCACAT CCGGCAGCAA CAGCAGCAAC AGATCCTGCG GCAACAGCAA 
CAACAACAAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAGCA GCAGCAGCAA 
CAACAACAAC AACAACACCA GCAGCAGCAG CAACAGGCAG CTCCTCCCCA ACCCCAGCCC 
CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG CTTCAGCAGA CACAACAGCA GCAACAGACA 
GCAGCTTTGG TCCGGCAACT CCAACAACAG CTCTCCAATA CCCAGCCACA ACCCAGTACC 
AACATATTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005340023.1
CDS	16..6576
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTGRSQNAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTSAYYAAITETKVKKRHVDPFIEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHDFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGMSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPVDDPERKEAEGSSSSKLEDPALSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTANNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYNQVNQIVNNWREDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLQKELGEHQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKLSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSISHLSYRQGPIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPAYPGVLPTTVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTTMLGTMTPLNTQGVQAGnullRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_005339966.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339966.2

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGCCTGGGG 
CCTCCGGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTATCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTACC TGACACTGGT CGCAGGAAGC CTCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGG GCGATCCCAG AATGCCATTA ACACCTGGTT CACTGATCTG 
GCTGGCACTA AACCGCTCAC ACAATTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GATACTTAGC CAAATACACA GTGCCTGTGA TGAGGGCCGC CTGGCTCATT 
AAGATGACCT CTGCCTACTA TGCAGCAATC ACTGAAACCA AGGTTAAGAA GAGACATGTT 
GACCCCTTCA TTGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GCTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC TACAATAGGG 
CCCTTGCCCC ATGATGTGGA GGTGGCAATC AGGCAGTGGG ACTACAATGA GAAGCTAGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGATT TCCTGACCTG GGTTCTAGAA 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGTTGCT GCCCCTACTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTGT CCCGCCGCCT TGCCTACTTT 
TGTACCCGGA GACTGGCCCT GCAACTGGAT GGCATGAGTA GCCACTCATC TCATGTTATA 
TCTGCTCAGT CGACAAGCAC ACTGCCTACC ACTCCTGCCC CTCAGCCCCC AACTAGCAGC 
ACACCCTCCA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGTCTCAGCT GTATACTACA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAT 
TATTCACTGA CTGATAGCCG AATTAAGACG GGTTCACCAC TTGACCACCT GCCTATTGCC 
CCTTCCAACC TGCCCATGCC AGAAGGCAAC AGTGCCTTCA CTCAGCAAGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC CACTGCAGGC TTTACCATTG GACGGGTGCT CCATACTTTG 
GAAGTTCTGG ACAGTCATAG TTTTGAGCGC TCCGACTTCA GCAACTCTCT TGACTCTCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC TTCTGATGAT 
GATGCTGTGG TTTCATTATT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGTCAT 
CGTGCCATGG TGGTAGCCAA GCTTCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CCGAAGCAGC AGATGAGAAG GGTTCCATTG CTTCTGGTTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGATC CCCGAAGTGA GAGCGAGCGG GTGGAATTCT TTAACTTGGT ACTGCTTTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTAATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCGCGGCCTC CCTCTCCCTT TGATGATCCT 
GTCGATGACC CAGAGCGCAA GGAGGCTGAA GGTAGCAGCA GTAGCAAGCT GGAGGATCCA 
GCACTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGTTGTTTGA GGATATGGAG 
AAGCCTGACT TCTCATTGTT TTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGCCCATCT 
CCTGAGAAAC CAGATGTAGA GAAGGAGGTG AAGCCACCAC CCAAGGAGAA GATTGAAGGA 
ACCCTTGGGG TTCTTTATGA CCAGCCACGA CATGTGCAGT ACGCCACACA CTTTCCTATT 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTTGTACT ATTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGCCG CAACCGACCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AACATCTTTC ACATTATGAC 
CAACACCAGG TCACTGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTGGTTG AAGCCGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTACC ATGCCTGTCT CATCCTGAAC 
CAGGACCAGA TGGCGCAGGT CTTTGAGGGG CTGTGTGGTG TAGTGAAGCA TGGGATGAAC 
CGATCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAATACCA TCTACTGTAA TGTGGAGCCA TCAGAATCTA ATATGCGCTG GGCACCCGAG 
TTTATGATCG ACACCCTGGA GAACCCTGCT GCTCATACCT TCACCTACAC AGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTAG GGCACCATGA TCCCGATAGG GTGAATGACA TTGCAATCCT GTGTGCAGAG 
CTGACCGGCT ACTGCAAGTC ACTAAGTGCT GAGTGGCTAG GAGTACTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGCGGTTTC AATGATCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCCT TTCATGACTC CTTGGCTACT TTTGTTGCCA TCCTCATTGC TCGCCAGTGT 
TTGCTCCTAG AAGATCTGAT CCGCTGTGCT GCCATCCCGT CACTCCTGAA TGCTGCTTGT 
AGTGAGCAGG ACTCAGAGCC TGGGGCCCGG CTTACTTGCC GCATCCTTCT CCACCTTTTC 
AAGACACCAC AACTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT CGGAATCCGC 
TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAAGAGGAGG GAGGAGGTGG CAGTGGCGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCGCTA 
TGTGAGGACA GCAATGACCT ACAGGACCCA GTGTTGAGTA GCGCCCAGGC CCAGCGCCTC 
ATGCAGCTCA TCTGCTACCC ACATCGACTT CTGGACAATG AGGATGGGGA AAACCCCCAG 
AGGCAGCGCA TTAAGCGTAT TCTCCAGAAT TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAAGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCA 
GGAAGCACTG CGAACAACAT GCCCAGCAGC AGCAAGACCA AACCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCCA AGCTGCCCAC CTCAGTCCAA 
GGGCATGTAT TAAAGGCTGC TGGAGAGGAA CTGGAGAAGG GTCAGCATCT GGGCTCTTCT 
TCTCGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTGTTGAG CCAGCAGCCC 
TTCTTATCCC TGGTGCTGAC ATGTCTGAAA GGGCAAGATG AGCAACGTGA GGGACTCCTT 
ACCTCCCTCT ACAACCAAGT GAACCAGATT GTGAATAATT GGCGAGAGGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACAGT GCAACGCAGC ACCCAGCAGA CCACAGAGTG GGCCATGCTC 
CTCCTGGAAA TCATCATCAG TGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTTACT 
ACTGTGTTGG ACATGCTGAG TGTACTCATC AATGGAACCT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GCAGCATGGA GGAAAACAAA CGTGCATATA TGAACCTAGT GAAGAAGCTG 
CAGAAGGAAT TGGGAGAGCA TCAATCAGAC AGTCTGGAAA AGGTTCGCCA GTTGCTACCA 
CTGCCCAAGC AGACTAGAGA TGTCATCACG TGTGAACCAC AGGGCTCCCT CATTGACACC 
AAAGGCAACA AGATTGCTGG CTTTGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACAAAACAGA AGATATCTCC CTGGGATCTC TTCGAGGGCT TGAAGCTCTC TGCACCACTC 
TCCTGGGGCT GGTTTGGGAC AGTCCGAGTT GACCGGCGAG TGGCTCGAGG GGAGGAGCAG 
CAGCGCTTGC TACTCTACCA CACACACCTG AGGCCCCGGC CCCGTGCCTA TTACCTAGAG 
CCACTGCCAC TGCCTCCAGA AGATGAGGAG CCACCAGCTC CCACCCTGCT AGAACCTGAG 
AAAAAGGCTC CTGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCCAC CAAGACAGAG 
GACTATGGCA TGGGCCCAGG CCGAAGTGGT CCCTATGGTG TGACAGTGCC TCCAGACCTC 
CTGCACCACC CCAACCCTGG ATCCATATCT CACCTGAGCT ACAGGCAGGG CCCCATAGGC 
CTGTATACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATATCGCCCT 
GTGAGGTTAC CAATGCAGAA GCTGCCAACC CGACCAGCTT ACCCTGGAGT GCTACCCACA 
ACTGTGACTG GTGTTATGGG CCTAGAACCT TCATCTTACA AGACTTCCGT GTACCGACAG 
CAGCAACCTG CAGTGCCCCA GGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGGA TGTTGGGACA GTCGTCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGC 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTATCA GAGCACCCAC 
CCCTCTACCA ATCCTACTCT TGTAGATCCT ACCCGTCACC TGCAACAGCG GCCCAGTGGC 
TATGTACACC AGCAGGCCCC AACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC CACCATGCTA GGTACCATGA CTCCACTGAA TACCCAGGGC 
GTCCAGGCAG GTNNCCGCTC AACTTCCATC CTACCTGAGC AGCAGCAGCA GCAACAACAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGTACCACAT CCGGCAGCAA CAGCAGCAAC AGATCCTGCG GCAACAGCAA 
CAACAACAAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAGCA GCAGCAGCAA 
CAACAACAAC AACAACACCA GCAGCAGCAG CAACAGGCAG CTCCTCCCCA ACCCCAGCCC 
CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG CTTCAGCAGA CACAACAGCA GCAACAGACA 
GCAGCTTTGG TCCGGCAACT CCAACAACAG CTCTCCAATA CCCAGCCACA ACCCAGTACC 
AACATATTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med12 ( XM_005339966.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005340023.1 Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.

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CloneID	OIc70762
Clone ID Related Accession (Same CDS sequence)	XM_005339966.2 , XM_005339966.3
Accession Version	XM_005339966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936762.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005339966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGCCTGGGG 
CCTCCGGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTATCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTACC TGACACTGGT CGCAGGAAGC CTCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGG GCGATCCCAG AATGCCATTA ACACCTGGTT CACTGATCTG 
GCTGGCACTA AACCGCTCAC ACAATTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GATACTTAGC CAAATACACA GTGCCTGTGA TGAGGGCCGC CTGGCTCATT 
AAGATGACCT CTGCCTACTA TGCAGCAATC ACTGAAACCA AGGTTAAGAA GAGACATGTT 
GACCCCTTCA TTGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GCTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC TACAATAGGG 
CCCTTGCCCC ATGATGTGGA GGTGGCAATC AGGCAGTGGG ACTACAATGA GAAGCTAGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGATT TCCTGACCTG GGTTCTAGAA 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGTTGCT GCCCCTACTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTGT CCCGCCGCCT TGCCTACTTT 
TGTACCCGGA GACTGGCCCT GCAACTGGAT GGCATGAGTA GCCACTCATC TCATGTTATA 
TCTGCTCAGT CGACAAGCAC ACTGCCTACC ACTCCTGCCC CTCAGCCCCC AACTAGCAGC 
ACACCCTCCA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGTCTCAGCT GTATACTACA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAT 
TATTCACTGA CTGATAGCCG AATTAAGACG GGTTCACCAC TTGACCACCT GCCTATTGCC 
CCTTCCAACC TGCCCATGCC AGAAGGCAAC AGTGCCTTCA CTCAGCAAGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC CACTGCAGGC TTTACCATTG GACGGGTGCT CCATACTTTG 
GAAGTTCTGG ACAGTCATAG TTTTGAGCGC TCCGACTTCA GCAACTCTCT TGACTCTCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC TTCTGATGAT 
GATGCTGTGG TTTCATTATT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGTCAT 
CGTGCCATGG TGGTAGCCAA GCTTCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CCGAAGCAGC AGATGAGAAG GGTTCCATTG CTTCTGGTTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGATC CCCGAAGTGA GAGCGAGCGG GTGGAATTCT TTAACTTGGT ACTGCTTTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTAATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCGCGGCCTC CCTCTCCCTT TGATGATCCT 
GTCGATGACC CAGAGCGCAA GGAGGCTGAA GGTAGCAGCA GTAGCAAGCT GGAGGATCCA 
GCACTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGTTGTTTGA GGATATGGAG 
AAGCCTGACT TCTCATTGTT TTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGCCCATCT 
CCTGAGAAAC CAGATGTAGA GAAGGAGGTG AAGCCACCAC CCAAGGAGAA GATTGAAGGA 
ACCCTTGGGG TTCTTTATGA CCAGCCACGA CATGTGCAGT ACGCCACACA CTTTCCTATT 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTTGTACT ATTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGCCG CAACCGACCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AACATCTTTC ACATTATGAC 
CAACACCAGG TCACTGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTGGTTG AAGCCGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTACC ATGCCTGTCT CATCCTGAAC 
CAGGACCAGA TGGCGCAGGT CTTTGAGGGG CTGTGTGGTG TAGTGAAGCA TGGGATGAAC 
CGATCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAATACCA TCTACTGTAA TGTGGAGCCA TCAGAATCTA ATATGCGCTG GGCACCCGAG 
TTTATGATCG ACACCCTGGA GAACCCTGCT GCTCATACCT TCACCTACAC AGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTAG GGCACCATGA TCCCGATAGG GTGAATGACA TTGCAATCCT GTGTGCAGAG 
CTGACCGGCT ACTGCAAGTC ACTAAGTGCT GAGTGGCTAG GAGTACTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGCGGTTTC AATGATCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCCT TTCATGACTC CTTGGCTACT TTTGTTGCCA TCCTCATTGC TCGCCAGTGT 
TTGCTCCTAG AAGATCTGAT CCGCTGTGCT GCCATCCCGT CACTCCTGAA TGCTGCTTGT 
AGTGAGCAGG ACTCAGAGCC TGGGGCCCGG CTTACTTGCC GCATCCTTCT CCACCTTTTC 
AAGACACCAC AACTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT CGGAATCCGC 
TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAAGAGGAGG GAGGAGGTGG CAGTGGCGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCGCTA 
TGTGAGGACA GCAATGACCT ACAGGACCCA GTGTTGAGTA GCGCCCAGGC CCAGCGCCTC 
ATGCAGCTCA TCTGCTACCC ACATCGACTT CTGGACAATG AGGATGGGGA AAACCCCCAG 
AGGCAGCGCA TTAAGCGTAT TCTCCAGAAT TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAAGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCA 
GGAAGCACTG CGAACAACAT GCCCAGCAGC AGCAAGACCA AACCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCCA AGCTGCCCAC CTCAGTCCAA 
GGGCATGTAT TAAAGGCTGC TGGAGAGGAA CTGGAGAAGG GTCAGCATCT GGGCTCTTCT 
TCTCGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTGTTGAG CCAGCAGCCC 
TTCTTATCCC TGGTGCTGAC ATGTCTGAAA GGGCAAGATG AGCAACGTGA GGGACTCCTT 
ACCTCCCTCT ACAACCAAGT GAACCAGATT GTGAATAATT GGCGAGAGGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACAGT GCAACGCAGC ACCCAGCAGA CCACAGAGTG GGCCATGCTC 
CTCCTGGAAA TCATCATCAG TGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTTACT 
ACTGTGTTGG ACATGCTGAG TGTACTCATC AATGGAACCT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GCAGCATGGA GGAAAACAAA CGTGCATATA TGAACCTAGT GAAGAAGCTG 
CAGAAGGAAT TGGGAGAGCA TCAATCAGAC AGTCTGGAAA AGGTTCGCCA GTTGCTACCA 
CTGCCCAAGC AGACTAGAGA TGTCATCACG TGTGAACCAC AGGGCTCCCT CATTGACACC 
AAAGGCAACA AGATTGCTGG CTTTGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACAAAACAGA AGATATCTCC CTGGGATCTC TTCGAGGGCT TGAAGCTCTC TGCACCACTC 
TCCTGGGGCT GGTTTGGGAC AGTCCGAGTT GACCGGCGAG TGGCTCGAGG GGAGGAGCAG 
CAGCGCTTGC TACTCTACCA CACACACCTG AGGCCCCGGC CCCGTGCCTA TTACCTAGAG 
CCACTGCCAC TGCCTCCAGA AGATGAGGAG CCACCAGCTC CCACCCTGCT AGAACCTGAG 
AAAAAGGCTC CTGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCCAC CAAGACAGAG 
GACTATGGCA TGGGCCCAGG CCGAAGTGGT CCCTATGGTG TGACAGTGCC TCCAGACCTC 
CTGCACCACC CCAACCCTGG ATCCATATCT CACCTGAGCT ACAGGCAGGG CCCCATAGGC 
CTGTATACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATATCGCCCT 
GTGAGGTTAC CAATGCAGAA GCTGCCAACC CGACCAGCTT ACCCTGGAGT GCTACCCACA 
ACTGTGACTG GTGTTATGGG CCTAGAACCT TCATCTTACA AGACTTCCGT GTACCGACAG 
CAGCAACCTG CAGTGCCCCA GGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGGA TGTTGGGACA GTCGTCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGC 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTATCA GAGCACCCAC 
CCCTCTACCA ATCCTACTCT TGTAGATCCT ACCCGTCACC TGCAACAGCG GCCCAGTGGC 
TATGTACACC AGCAGGCCCC AACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC CACCATGCTA GGTACCATGA CTCCACTGAA TACCCAGGGC 
GTCCAGGCAG GTNNCCGCTC AACTTCCATC CTACCTGAGC AGCAGCAGCA GCAACAACAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGTACCACAT CCGGCAGCAA CAGCAGCAAC AGATCCTGCG GCAACAGCAA 
CAACAACAAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAGCA GCAGCAGCAA 
CAACAACAAC AACAACACCA GCAGCAGCAG CAACAGGCAG CTCCTCCCCA ACCCCAGCCC 
CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG CTTCAGCAGA CACAACAGCA GCAACAGACA 
GCAGCTTTGG TCCGGCAACT CCAACAACAG CTCTCCAATA CCCAGCCACA ACCCAGTACC 
AACATATTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005340023.1
CDS	70..6630
Translation	MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTGRSQNAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTSAYYAAITETKVKKRHVDPFIEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYNEKLAMFMFQDGMLDRHDFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGMSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPVDDPERKEAEGSSSSKLEDPALSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTANNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYNQVNQIVNNWREDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLQKELGEHQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKLSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSISHLSYRQGPIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPAYPGVLPTTVTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTTMLGTMTPLNTQGVQAGnullRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	XM_005339966.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus mediator complex subunit 12 (Med12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339966.3

ORF Insert Sequence:

ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGCCTGGGG 
CCTCCGGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTATCTGGGG ATGAACATGG CAGTGCCAAG 
AACGTCAACT TTAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAG 
TTACGTTGTA ACACCCTACC TGACACTGGT CGCAGGAAGC CTCAAGTGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGG GCGATCCCAG AATGCCATTA ACACCTGGTT CACTGATCTG 
GCTGGCACTA AACCGCTCAC ACAATTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA 
GAAGTGTTTG GATACTTAGC CAAATACACA GTGCCTGTGA TGAGGGCCGC CTGGCTCATT 
AAGATGACCT CTGCCTACTA TGCAGCAATC ACTGAAACCA AGGTTAAGAA GAGACATGTT 
GACCCCTTCA TTGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GCTACAGAAG 
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC TACAATAGGG 
CCCTTGCCCC ATGATGTGGA GGTGGCAATC AGGCAGTGGG ACTACAATGA GAAGCTAGCC 
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGATT TCCTGACCTG GGTTCTAGAA 
TGTTTTGAGA AAATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGTTGCT GCCCCTACTG 
CTTCGATACT CTGGGGAATT CGTTCAGTCT GCATACCTGT CCCGCCGCCT TGCCTACTTT 
TGTACCCGGA GACTGGCCCT GCAACTGGAT GGCATGAGTA GCCACTCATC TCATGTTATA 
TCTGCTCAGT CGACAAGCAC ACTGCCTACC ACTCCTGCCC CTCAGCCCCC AACTAGCAGC 
ACACCCTCCA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT 
GGTCTCAGCT GTATACTACA GACCATCCTC CTGTGTTGTC CTAGTGCCCT GGTTTGGCAT 
TATTCACTGA CTGATAGCCG AATTAAGACG GGTTCACCAC TTGACCACCT GCCTATTGCC 
CCTTCCAACC TGCCCATGCC AGAAGGCAAC AGTGCCTTCA CTCAGCAAGT CCGTGCAAAG 
TTGCGGGAGA TTGAGCAGCA GATCAAGGAG CGAGGACAGG CAGTTGAGGT TCGCTGGTCT 
TTTGATAAGT GCCAGGAAGC CACTGCAGGC TTTACCATTG GACGGGTGCT CCATACTTTG 
GAAGTTCTGG ACAGTCATAG TTTTGAGCGC TCCGACTTCA GCAACTCTCT TGACTCTCTT 
TGTAACCGAA TCTTTGGATT GGGGCCTAGC AAGGATGGGC ACGAGATCTC TTCTGATGAT 
GATGCTGTGG TTTCATTATT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGTCAT 
CGTGCCATGG TGGTAGCCAA GCTTCTGGAG AAGAGACAGG CAGAGATTGA GGCTGAGCGT 
TGTGGAGAAT CCGAAGCAGC AGATGAGAAG GGTTCCATTG CTTCTGGTTC CCTTTCTGCT 
CCTAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG 
CTGACGGATC CCCGAAGTGA GAGCGAGCGG GTGGAATTCT TTAACTTGGT ACTGCTTTTC 
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTAATCTCC 
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCGCGGCCTC CCTCTCCCTT TGATGATCCT 
GTCGATGACC CAGAGCGCAA GGAGGCTGAA GGTAGCAGCA GTAGCAAGCT GGAGGATCCA 
GCACTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TGTTGTTTGA GGATATGGAG 
AAGCCTGACT TCTCATTGTT TTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGCCCATCT 
CCTGAGAAAC CAGATGTAGA GAAGGAGGTG AAGCCACCAC CCAAGGAGAA GATTGAAGGA 
ACCCTTGGGG TTCTTTATGA CCAGCCACGA CATGTGCAGT ACGCCACACA CTTTCCTATT 
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTTGTACT ATTTGGGGTG 
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCCTGAAG 
GTTCTGAACC GCAAGGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT 
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGCCG CAACCGACCT 
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AACATCTTTC ACATTATGAC 
CAACACCAGG TCACTGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC 
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG 
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG 
AGTGTGGTTG AAGCCGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACC 
AGCCTGTGCC TGTGCATTGT GGCTGTCCTG CGGCACTACC ATGCCTGTCT CATCCTGAAC 
CAGGACCAGA TGGCGCAGGT CTTTGAGGGG CTGTGTGGTG TAGTGAAGCA TGGGATGAAC 
CGATCAGATG GCTCCTCTGC AGAACGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC 
TCCTGTAGCC ATTTAAAGAG CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG 
AAGAATACCA TCTACTGTAA TGTGGAGCCA TCAGAATCTA ATATGCGCTG GGCACCCGAG 
TTTATGATCG ACACCCTGGA GAACCCTGCT GCTCATACCT TCACCTACAC AGGGCTAGGC 
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC 
GTCTGTGTAG GGCACCATGA TCCCGATAGG GTGAATGACA TTGCAATCCT GTGTGCAGAG 
CTGACCGGCT ACTGCAAGTC ACTAAGTGCT GAGTGGCTAG GAGTACTTAA GGCCTTGTGC 
TGCTCCTCTA ACAATGGCAC TTGCGGTTTC AATGATCTCC TCTGCAATGT AGATGTCAGT 
GACCTGTCCT TTCATGACTC CTTGGCTACT TTTGTTGCCA TCCTCATTGC TCGCCAGTGT 
TTGCTCCTAG AAGATCTGAT CCGCTGTGCT GCCATCCCGT CACTCCTGAA TGCTGCTTGT 
AGTGAGCAGG ACTCAGAGCC TGGGGCCCGG CTTACTTGCC GCATCCTTCT CCACCTTTTC 
AAGACACCAC AACTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT CGGAATCCGC 
TCCTCCTGTG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCTGTG 
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAGGG TTCAGGCTTC 
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAAGAGGAGG GAGGAGGTGG CAGTGGCGGT 
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG 
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCGCTA 
TGTGAGGACA GCAATGACCT ACAGGACCCA GTGTTGAGTA GCGCCCAGGC CCAGCGCCTC 
ATGCAGCTCA TCTGCTACCC ACATCGACTT CTGGACAATG AGGATGGGGA AAACCCCCAG 
AGGCAGCGCA TTAAGCGTAT TCTCCAGAAT TTGGACCAGT GGACCATGCG CCAGTCTTCC 
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG 
AACATCGCCA AGGCCACAAT CGAAGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCA 
GGAAGCACTG CGAACAACAT GCCCAGCAGC AGCAAGACCA AACCTGTGCT CAGCTCTCTA 
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCCA AGCTGCCCAC CTCAGTCCAA 
GGGCATGTAT TAAAGGCTGC TGGAGAGGAA CTGGAGAAGG GTCAGCATCT GGGCTCTTCT 
TCTCGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTGTTGAG CCAGCAGCCC 
TTCTTATCCC TGGTGCTGAC ATGTCTGAAA GGGCAAGATG AGCAACGTGA GGGACTCCTT 
ACCTCCCTCT ACAACCAAGT GAACCAGATT GTGAATAATT GGCGAGAGGA CCAGTACTTA 
GATGATTGCA AACCAAAGCA GCTAATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG 
GGGGGCATGT TTGACACAGT GCAACGCAGC ACCCAGCAGA CCACAGAGTG GGCCATGCTC 
CTCCTGGAAA TCATCATCAG TGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTTACT 
ACTGTGTTGG ACATGCTGAG TGTACTCATC AATGGAACCT TGGCTGCAGA CATGTCTAGC 
ATCTCACAAG GCAGCATGGA GGAAAACAAA CGTGCATATA TGAACCTAGT GAAGAAGCTG 
CAGAAGGAAT TGGGAGAGCA TCAATCAGAC AGTCTGGAAA AGGTTCGCCA GTTGCTACCA 
CTGCCCAAGC AGACTAGAGA TGTCATCACG TGTGAACCAC AGGGCTCCCT CATTGACACC 
AAAGGCAACA AGATTGCTGG CTTTGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC 
ACAAAACAGA AGATATCTCC CTGGGATCTC TTCGAGGGCT TGAAGCTCTC TGCACCACTC 
TCCTGGGGCT GGTTTGGGAC AGTCCGAGTT GACCGGCGAG TGGCTCGAGG GGAGGAGCAG 
CAGCGCTTGC TACTCTACCA CACACACCTG AGGCCCCGGC CCCGTGCCTA TTACCTAGAG 
CCACTGCCAC TGCCTCCAGA AGATGAGGAG CCACCAGCTC CCACCCTGCT AGAACCTGAG 
AAAAAGGCTC CTGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG 
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCCAC CAAGACAGAG 
GACTATGGCA TGGGCCCAGG CCGAAGTGGT CCCTATGGTG TGACAGTGCC TCCAGACCTC 
CTGCACCACC CCAACCCTGG ATCCATATCT CACCTGAGCT ACAGGCAGGG CCCCATAGGC 
CTGTATACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATATCGCCCT 
GTGAGGTTAC CAATGCAGAA GCTGCCAACC CGACCAGCTT ACCCTGGAGT GCTACCCACA 
ACTGTGACTG GTGTTATGGG CCTAGAACCT TCATCTTACA AGACTTCCGT GTACCGACAG 
CAGCAACCTG CAGTGCCCCA GGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG 
AGTCAGGGGA TGTTGGGACA GTCGTCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGC 
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA 
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTATCA GAGCACCCAC 
CCCTCTACCA ATCCTACTCT TGTAGATCCT ACCCGTCACC TGCAACAGCG GCCCAGTGGC 
TATGTACACC AGCAGGCCCC AACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA 
CACCAGACAC TGCAGCAGAC CACCATGCTA GGTACCATGA CTCCACTGAA TACCCAGGGC 
GTCCAGGCAG GTNNCCGCTC AACTTCCATC CTACCTGAGC AGCAGCAGCA GCAACAACAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGTACCACAT CCGGCAGCAA CAGCAGCAAC AGATCCTGCG GCAACAGCAA 
CAACAACAAC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AACAACAGCA GCAGCAGCAA 
CAACAACAAC AACAACACCA GCAGCAGCAG CAACAGGCAG CTCCTCCCCA ACCCCAGCCC 
CAGTCCCAGC CCCAGTTCCA GCGCCAGGGG CTTCAGCAGA CACAACAGCA GCAACAGACA 
GCAGCTTTGG TCCGGCAACT CCAACAACAG CTCTCCAATA CCCAGCCACA ACCCAGTACC 
AACATATTCG GACGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Med12 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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