Frem2 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frem2
Entrez Gene ID	101960884
Full Name	FRAS1 related extracellular matrix protein 2
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005330232.2	XP_005330289.1	FRAS1-related extracellular matrix protein 2
XM_005330232.3	XP_005330289.1	FRAS1-related extracellular matrix protein 2

Pathways from BioSystems
Gene Ontology

The following Frem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc61233	XM_005330232.3 Latest version!	Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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OIc61233	XM_005330232.2	Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frem2 ( XM_005330232.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005330289.1 Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.

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CloneID	OIc61233
Clone ID Related Accession (Same CDS sequence)	XM_005330232.2 , XM_005330232.3
Accession Version	XM_005330232.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	FRAS1-related extracellular matrix protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936565.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005330232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCAG GCAGAACCCC AGAGGTCGCT ACCCGCGCGC GCCGGACAGC CAACTCCGCC 
AGCTCTCAGC CAAAACTTGT TCCGCCACCC AGTCTGCTGC TGCTTCTCCT ATCACTGGTA 
AGCCGCATCT CGGCACAGCT CGCCGTGGGT GACCGAGCAT TGCTGTCTCC GGGTCTCGCG 
GGGGCTGCGG GGGTTCCTTC CGAGGAGGCC ATAGTGATGG CGAATCGCGG ACTCAGGGTG 
CCTTTCGGGC GTGAAGTGTG GCTCGATCCG CTGCACGACC TAGTGTTGCA GGTGCAGCCA 
GGGGACCGCT GCGCGGTGAC TGTGCTAGAC AACGACGCGC TGGCTCAGAG GCCCGGCCGC 
CTGAGTCCCA AGCGCTTCCC CTGCGACTTT GGCCCAGGAG AGGTGCGCTA TTCCCACCTG 
GGTGCGCGCA GCCCGTCGCG GGACCGCGTG CGACTGCAGC TGCGCTATGA CGCTCCTGGA 
GGGGCAGTAG TGCTACCACT GGTCCTGGAG GTGGAAGTAG TCTTCACCCA ACTGGAGATT 
GTGACTCGGA ACTTGCCTCT GGTCGTGGAA GAACTGCTGG GGACCAGCAA TGCCCTGGAC 
GCGCGGAGCT TGGAGTTTGC CTACCAGTCG GAGACCGAGG AGTGTCGCGT GGGCATCCTG 
ACGGGCTTGA GTGCGCTGCC TCGCTATGGA GAACTCCTCC ACTATCCGCA GGTCCCTGGA 
GAAGCCAGAG AAGGAAGTAC CTCGGAGCCT CTCTTGATGG ACTGCAAAGC TTTTCAGGAG 
CTAGGTGTGC GCTACCGTCA CACAGCCCCC AGTCGCTCAC CCAACAGAGA TTGGGTACCC 
ATGGTGGTGG AGCTGCGTTC ACGAGGGGCT CCTCTTGGCA GCCCTGCTCT AAAACGCGAG 
CACTTTCAGG TGCTAGTGAG GATCCGAGGA GGGGCTGAGA ACACCGCACC TAAGCCCAGT 
TTTGTGGCCA TGATGATGAT GGAGGTGGAC CAGTTTGTAC TGACCGCCCT GACTCCAGAC 
ATGCTGGCAG CTGAGGATGC TGAGTCTACC CCTGACCTAT TGATCTTCAA CCTCACTGCT 
CCCTTACAGC CCGGCCAGGG TTACTTGGTG AGCACCGATG ACCGCAGCCT ACCCCTCTCC 
TCCTTCACTC AGAGAGATCT GCACCTTCTG AAGATAGCCT ACCAGCCCCC TTCTGAAGAC 
TCTAACCAGG AGCGACTCTT TGAACTGGAG CTAGAAGTAG TGGACCCAGA AGGAGCAGCC 
TCCGATCCTT TTGCTTTCAT GGTAGTGGTG AAGCCTATGA ACACACTGGC TCCGGTGGTC 
ACACGAAACA CTGGTCTTAT TCTGTACGAA GGTCAGTCTC GGCCTCTCAC AGGTCCTGTA 
GGTAGTGGAC CACAAAACTT GGTCATCAGT GATGAGGATG ATCTAGAAGC AGTGCGACTG 
GAGGTGGTGG CTGGGCTCCG GCATGGTCAC CTTGTCATTC TGGGTGCTCC CAGCGGCAAC 
TCTGCTCCTA AGACTTTTAC AGTGGCAGAG CTAGCAGCTG GCCATGTGGT TTACCAGCAT 
GATGACAAAG ATGGTTCACT GAGTGACAAC CTGGTGCTCC GCATGGTGGA TGGAGGAGGC 
AGACACCAGG TGCAGTTCCT GTTCCCCATC ACTTTAGTGC CTGTGGATGA CCAGCCACCT 
GTCCTGAATG CCAACACTGG ACTGACATTG GCAGAAGGTG AAACAGTGCC TATCCCACCG 
ATTGCTCTGA GTGCAACTGA CATAGATTCT GATGACTCTC TGCTGCTTTT TGTGCTGGAG 
TCACCCTCCT CAACTGCAGG GCATCTGCTT CTCCGCCAAA CTCATCCTCC CCAAGAGGAG 
CAGGAGCTGA TCAGTGGCCT TTGGAGGAAG CAGGGGGCAT ATTATGAACG AACAGTGACA 
GAGTGGCAGC AGAAAGACAT AACAGAAGGG CGGCTCTTCT ATAGACACTC TGGGCCCCAT 
AGTCCTGGCC CAGTGATGGA CCAGTTTACA TTTAGAGTTC AGGATAACCA TGACCCCCCT 
AATCAATCTG GACTACAGAA GTTTGTCATA CGTATTCATC CTGTGGATCG CCTCCCTCCA 
GAGCTGGTCA GTGGCTGTCC CCTTCGGATG GTGGTGCAGG AATCCCAGCT CACACCACTG 
AGGAAGAAGT GGCTGCGCTA CACTGATCTG GACACTGATG ATAGAGAACT CCGTTACACC 
GTGACCCAGC CCCCTGTGGA CACCGATGAA AACCACTCAC CTGTCCCACT GGGCACCTTG 
GTCCTGACTG ATTACCCATC CACTGTGGTG ACCCATTTTA CCCAAGCCCA GATCAATCAC 
CATAAAATTG CATACAGACC CCCAGGTCAA GAACTGGGAG TGGCTGCTCG AGTGGCCCAG 
TTCCAGTTCC AGGTGGAGGA CCGAGCTGGG AATGTGGCTC CTGGTACCTT CACCCTTTAC 
TTGCAGCCCG TGGACAACCA GCCACCTGAT ATCCTCAACA CTGGCTTTAC TATTCAGGAG 
AAGGGCCACC ACATCCTAAG TGAGACTGAG TTGCATGTGA GTGATGTAGA CACTGATATG 
GCCCACATCA TTTTTACCCT CACACAGGCA CCCAAACATG GCCACATGCA AGTGTCCGGA 
CAGACACTGC ATGTAGGAGA GCTCTTCCAC TTGGAGGACA TAAAACAGGG GCGAATTTCC 
TATACTCATA ATGGAGATGA GTCCTTGACT GATAGCTGCT CCTTGGAGGT CAGTGACAGA 
CACCATGTGG TGCCTATCAC TCTCAGAGTA AATGTCCGGC CAGTGGACCA GGAGGTGCCC 
ATGCTGAGCC TTCCTACAGG TACTCTGGAG TCCTATCTAG ACGTCTTAGA AAACGGAGCT 
ACTGAAATCA CCACCAGTGT TATTAAGGGG ACTGATGAAC ACACTGATGA TTTGATGCTG 
ACTTTCCTCT TGGAAGATCC ACCCTTGTAT GGAGAAATCC TGGTCAATGG AGCTCCAGCA 
GAGCAGTTCA CCCAAAAGGA CCTCTTGGAG GGCTCCGTTG TCTATGCCCA CACCAGTGGT 
GAGATTGGCC TGTTGCCCAA AGGAGACTCT TTCAACCTGA GCCTGTCAGA AAAGTCTCAA 
GAATGGAGAA TGGGTGGCAA TACTATTCAA GGTGTCACGG TGTGGGTAAC CATCCTCCCT 
GTGGACAGCC AGGCCCCAGA AATTTCTGTG GGTGAACAGT TTACGGTAAT GGAAGGTGAT 
AAAGGTGTTA TAACCTCGAT GCACATCAGC GCTGAAGATA TTGACTCCCT GAATGATGAC 
CTCCTGTGCA CAATCGTTAT TCAGCCAACC TCAGGCTATG TGGAAAACAT TTCTCCAGCC 
CCAGGCTCTG AGAAGTCCAG GGCAGGGATT GCCATAAGTG CCTTCACTCT GAAAGATCTC 
AGGCAGGGCC ACATTAACTA TGTCCAGAGT GTCCACAAAG GGGTGGAACC TGTGGAGGAC 
CGGTTTGTAT TTCGTTGCTC TGATGGCATT AACTTTTCCG AGAGACATTT TTTCCCCATT 
GTAATCATCC CTACCAATGA TGAAAAGCCA GAAATGTTTA TGAGAGAATT TATAGTCATG 
GAAGGTATGA GTCTGGTGAT TGATACGCCC ATCCTCAATG CTGCTGATGC TGACATCCCC 
CCGGATGACT TAACCTTCAC TATTACCCGA TTTCCCACTC ATGGGCACAT CATGAGCCAG 
CAGGTAAATG GCACGGTTCT TGTCGAAAGC TTCACCTTGG ATCAGATCAT AGAGAGTTCC 
AGCATTATTT ATGAGCATGA TGACTCCGAG ACCCAGGAAG ACAGTTTTGT GATCAAGTTA 
ACGGATGGTG AGCACTCTCT GGAAAAGGTG GCCCTCATCA TGATCCTCCC TGTGGATGAC 
GAGACCCCCA GGATGGCCAT CAATAATGGA CTGGAGATAG AAATTGGGGA AACCAAAGTC 
ATCAACAACA AAATATTAAT GGCCACTGAT TTGGACTCTG AAGACAAATC CTTGGCTTAT 
ATCATTCGTT ATGGGCCAGG ACATGGTGTA TTACAGAGGC GAAAACTTAC AGGTGCCTTT 
GAAAATATCA CGCTGGGCAT GAATTTTACC CAGGATGAAG TGGACAGAAA CTTAATTCAG 
TATGTCCATT TTGGGCAAGA GGGCATCCGG GACCTAATTA AATTTGATGT GACTGATGGA 
ATAAACGCCC TCATTGATCG CTACTTTTTT GTGTCCATTG GGAGTGTTGA CATTGTCTTC 
CCTGATGTGA TTAGTAAGGG GGTGTCCTTG AAGGAAGGTG GTAAAGTCAC CCTCACAACA 
GACCTACTAA GCACAAGTGA CTTGAACAGC CCCGATGAAA ACTTGGTTTT TACCATCACG 
AGGGCCCCCA TGAGAGGTCA CCTAGAGTGC ACTGACCAAC CTGGAGTGTC CATCGCATCT 
TTCACTCAGC TCCAATTGGC TGGCAACAAA ATCTACTACA TCCACACAGC TGACGATGAG 
GTGAAGATGG ACAGCTTTGA GTTTCAAGTC ACTGACGGGC GTAACCCTGT CTTCCGAACG 
TTCCGTATCT CCATTAGTGA CGTGGACAAC AAAAAGCCAG TGGTCACCAT CCATAACTTG 
GTTGTCAGCG AAGGTGAAAA CAAGCTGATC ACGCCCTTTG AACTCACTGT TGAAGACAGA 
GATACTCCCG ACAAACTCCT GAAATTCACT GTCACCCAGG TGCCTGTTCA TGGTCATCTC 
GTTTTCAATA ATACCAAACC TGCCACGGCT TTTACCAAGC AAGACTTGAA TGAAAACTTA 
ATCAGCTACA AGCATGATGG CACGGAGTCG AGTGAAGACA GCTTCTCCTT CACGGTGACC 
GATGGCACCC ATACAGACTT TTATGTTTTC CCAGATACAG TGTTTGAAAC GAGGAGACCT 
CAAGTGATGA AGATCCAGAT CTTACCTGTT GACAATAGTG TCCCCCAAAT TGCAGTGAAT 
AAAGGGGCCT CTGTGCTTCG CAATCTGGCC ACTGGTCACT TGGGGTTCAT GATCACAAGC 
AAAATATTGA AAGTGGAGGA TAGAGACAGC CTGCACTTCT CTCTGAAGTT TACTGTGACA 
GAGGCACCTC GACATGGATA TCTCTTCAAC CTGGATAAAG GCAACCACAG CATCACTCAG 
TTTACTCAAG CTGACATTGA TGACATGAAA ATATGCTATG TCTTAAAAGA AGGGGCTAAT 
GCCACAAGTG ATGTGTTCCA TTTTGCAGTT GAAGATGGTG GTGGAAACAA GTTAACCAAT 
CAGCATTTTC GTCTGAATTG GGCATGGATC TCCTTCGAAA AGGAATATAA CCTGATCAAT 
GAGGACTCCA AATTTCTCGA TGTTGTTCTT AAACGTAGAG GTTACTTGGG AGAAACTTCA 
TTTATAAGTA TTGGCACAAG AGATGGGACG GCAGAAAAGG ACAAGGACTT TAAAGGCAAA 
GCACAGAAGC AAGTGCAGTT CAACCCTGGC CAGACCAGGG CCACGTGGAG AGTGCGTATC 
CTGAGTGACG GGGAACATGA GCAATCCGAG ACCTTCCAGG TGGTTCTCTC GGAGCCTGTG 
CTGGCTGCCC TGGAGTTCCC TGCAGTGACC ACGGTAGAAA TTGTTGACCC AGGAGATGAA 
TCAACTGTGT TCATTCCCCA GTCGGAATAC TCCATTGAAG AAGATGTGGG CGAGCTGTTC 
ATTCCCATCA GGAGGACAGG AGACGTCAGC CAGGAGTTCA TGGTGATCTG TTACACACAG 
CAAGGAACAG CGACCGGAAC TGTGCCAACT TCCGTGTTGT CTTATTCGGA TTACATATCC 
AGGCCCGAGG ACCATACCAG TGTTATTCGC TTTGACAAAG ATGAAAGGGA GAAAATGTGT 
CGGATAGTTG TGATTGATGA CTCCTTATAT GAAGAAGAGG AAACGTTCCG TGTCCTTTTG 
AGCATGCCCA TGGGTGGGAG AATTGGATCA GAGTTCCCAG GAGCTCAGGT TACAATCCTC 
CCCGACAGAG ATGATGAACC CAGCTTCTAC TTTGGGAATG TGCAGTACTC TGTGGACGAG 
AGCTCCGGCC ACGTAGAGGT GCAGGTGTGG AGAACAGGTA CCGACCTCTC CAGGCCTTCC 
AGTGTCACAG TGAGATCTCG GAAGACGGAT CCTCCTTCTG CAGATGCTGG GACAGACTAT 
GTGGGAATCA GTCGCAATTT AGATTTTGCA CCTGGAGTCA ACATGCAGAC TGTTCGTGTT 
ATCATTCTGG ATGACCTTGG ACAACCAGTG CTGGAGGGAA CTGAGAAATT TGAACTGGTG 
CTTCGTATGC CTGTGAATGC TGTCCTTGGG GAGCCCAGCA AAGCCACGGT GTTCATAAAT 
GACTCTGTCT CAGACTTGCC TAAGATGCAA TTCAAAGAAC GAGTTTACAC TGGCAGCGAA 
AGTGATGGGC ATATAGCTGC TATGATCCAC AGGAGTGGTG ATATCCAGTA CAGGTCTTCA 
GTGCGATGCT ATACACGGCA GGGGTCCGCA CAGGTGATGA TGGACTTTGA AGAACGCCCA 
AACACTGACG TCTCCATCAT CACATTCCTC CCTGGTGAGA TTGAAAAGCC ATGTGTCCTT 
GAGCTGATGG ATGACATACT CTACGAGGAG GTGGAAGAGC TCCGCCTGGT ACTTGGCACT 
CCGCAAAGCA GCTCTCCCTT TGGGGCTGCC GTTGGTGAAC AGAATGAAAC TCTGATAAGG 
ATCCGAGACG ATGCTGATAA GACTATTATC AAATTTGGAG AAACCAAATT TAGCGTCACC 
GAACCCAGAG AACCAGGAGA GTCAGTCGTG GTTGAAATTC CAGTGATTCG CCAAGGAGAC 
ACTTCGAAGG TCTCCATTGT GAGAGTCCAC ACCAAGGATG GCTCTGCCAC CTCCGGAGAA 
GACTATCACC CTGTGTCAGA AGAAATTGAG TTTAAGGAAG GAGAAACCCA GCACATTGTT 
GAAATTGAAG TAATCTTCGA TGGGGTGAGA GAGATGAGAG AGGCCTTCAC TGTTCACTTA 
AAACCTGATG AAAATATGAT AGCAGAGACC CAGGTGACCA AGGCCATTGT GTATATAGAA 
GAAATGAACA GCATGGCAGA TGTCACTTTT CCTTCTGTCC CTCAAATTGT GTCCTTGCTG 
ATGTATGACG ACACGGCCAA AGCTAAGGAG AGTGCAGGAC CTGTGTCGGG CTATCCCATC 
ATCTGCGTCA CAGCTTGCAA CCCCAAATAT TCAGACTACG ATAAAACGGG CTCTATTTGT 
GCAAGTGAGA ACATCAACGA CACTCTGACC CGCTACCGAT GGCTGATTAG CGCTCCTGCT 
GGCCCTGATG GTGTGACCAG CCCCATGAGA GAGGTGGACT TCGATACCTT TTTTACATCA 
TCCAAGATGA TCACCTTAGA CTCCATATAC TTTCAGCCTG GCTCTCGGGT GCAGTGTGCG 
GCTCGTGCTG TGAACACCAA CGGGAATGAA GGTCTGGAGC TGACAAGCCC TATCGTTACC 
ATTGCCAGGG AAGAAGGTCT TTGTCAACCC CGTGTGCCTG GAGTAGTGGG GGCTGAACCG 
TTCTCAGCTA AATTGCGCTA CACAGGGCCC GAGGACCCGG ACTACTCAAA CCTTATCAAG 
CTCACAGTCA CCATGCCACA CATAGATGGC ATGCTCCCGG TGATCTCCAC CAGAGAGCTC 
TCCAACTTCG AGCTGACCCT CAGCCCCGAT GGCACCAGAG TTGGGAACCA CAAGTGCTCC 
AACCTCCTGG ACTACACTGA GGTGAAGACC CACCATGGCT TCCTGACGGA TGCCACGAAG 
AATCCAGAAG TCATTGGAGA GTCGTATCCT TACCAGTACA GCTCACCTCT CAGAGGAAAC 
GGCACCTTAC GCTTCTACCG GAACCTCAAC CTGGAGGCCT GTTTGTGGGA GTTTGTCAGC 
TACTATGACA TGTCAGAGCT CCTCGCTGAC TGCGGAGGCA CCATTGGAAC GGACGGACAG 
GTCCTAAACC TAGTGCAGTC TTACGTGACC CTTCGAGTAC CTCTGTATGT GTCCTACGTG 
TTCCACTCCC CAGTGGGAGT AGGTGGCTGG CAGCATTTTG ATTTGAAGTC GGAGCTTCGT 
CTGACTTTCG TGTATGACAC TGCCATCTTG TGGAGCAATG GAATTGGCAG CCCACCAGGA 
GCCGAACTCC AAGGATCTCT CTACCCAACG AGCATGCGCA TCAGGGAAGA GGGGCGCCTG 
GCTGTGAACT TCAAGACCCA GGCTCAGTTC CATGGCTTGT TTGTGCTGTC ACATCCCGCA 
TCTTTTACCA GCTCAATGAT CATGTCAGCT GATCATCCAG GCCTGACCTT TTCCCTCCGC 
CTTGTAAGGA GTGAACCAAC CTATAACCAG CCTATTCAGC AATGGAGCTT TGTCTCTGAC 
TTTGCAGTAC GTGACTACTC GGGAACCTAT ACTGTAAAGC TGGTGCCATG CACCACCCCG 
TCCAATCAGG AGTACCGACT GCCAGTCACC TGCAACCCCA GAGAGCCTGT CACCTTTGAC 
CTTGACATCC GATTCCAACA GGTCAGCGAT CCAGTGGCAG CTGAATTCAG CCTCAATACT 
CAGATGTACC TACTCTCCAA GAAGAGTCTC TGGTTGTCTG ACGGATCCAT GGGATTTGGA 
CAAGAAAGCG ATGTTGCTTT TGCAGAAGGT GATACAATTT ATGGTCGTGT CATGGTAGAT 
CCTGTCCAGA ATCTGGGTGA CTCCTTTTAC TGCAGCATTG AGAAGGTTTT CCTATGCACT 
GGAGCTGATG GCTATGTCCC CAAGTATAGT CCAACAAATG AAGAATATGG CTGTTTAGCC 
GACTCTCCTT CACTTCTGTA TAGATTTAAA ATTGTGGACA AAGCTCAACC AGAGACACAA 
GCCACCAGTT TTGGAAATGT CCTCTTTAAT GCCAAACTGG CAGTGGATGA CCCTGAAGCC 
ATCCTCTTAG TGAATCAGCC TGGCTCTGAT GGATTTAAAG TCGACTCAAC ACCACTCTTT 
CAGGTTGCTC TGGGCCGAGA ATGGTACATA CACACAATCT ATACAGTAAG ATCAAAAGAC 
AACACCAATC GAGGTATCGG CAAAAGAAGT GTGGAGTATG AGTACCACTC TCTGGTGCAT 
CAAGGGAAGC CACAGATAAC TACCGGGAGC CGGAAGAAGA GAGAGATCAG GAGCGCACCC 
CCACTGGCAT GGGACATCGG TGCTGAAAAC AACCGAGGGA CAAACATCCA GCACATTGCC 
CTGGACCGCG CCAACAAGAG GCAGACCCCC CCAGGGAGAG TGCCCCCCGA AGGCATCCTA 
CCCCGGGAGC TCAACAGTCC CAGCTCTGAG GTCAGTCTGG TCACCATCGT GGGCAGCATC 
GCGGTGGGAT TACTCACCAT CTGCCTCACT GTCATTGCAG CAATGATGTG CAAAAGCAAG 
AAAAGTGTCA GGGGTAAGGA CACTCCGAAG GGCTCTGGCA GCAGCGAGCC CATGATGCCC 
CCGCAGAGCC ACCATAGCGA CAGCTCAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005330289.1
CDS	1..9516
Translation	MHPGRTPEVATRARRTANSASSQPKLVPPPSLLLLLLSLVSRISAQLAVGDRALLSPGLAGAAGVPSEEAIVMANRGLRVPFGREVWLDPLHDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAVVLPLVLEVEVVFTQLEIVTRNLPLVVEELLGTSNALDARSLEFAYQSETEECRVGILTGLSALPRYGELLHYPQVPGEAREGSTSEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGAPLGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESTPDLLIFNLTAPLQPGQGYLVSTDDRSLPLSSFTQRDLHLLKIAYQPPSEDSNQERLFELELEVVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGPVGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGAPSGNSAPKTFTVAELAAGHVVYQHDDKDGSLSDNLVLRMVDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPIPPIALSATDIDSDDSLLLFVLESPSSTAGHLLLRQTHPPQEEQELISGLWRKQGAYYERTVTEWQQKDITEGRLFYRHSGPHSPGPVMDQFTFRVQDNHDPPNQSGLQKFVIRIHPVDRLPPELVSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPVDTDENHSPVPLGTLVLTDYPSTVVTHFTQAQINHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPDILNTGFTIQEKGHHILSETELHVSDVDTDMAHIIFTLTQAPKHGHMQVSGQTLHVGELFHLEDIKQGRISYTHNGDESLTDSCSLEVSDRHHVVPITLRVNVRPVDQEVPMLSLPTGTLESYLDVLENGATEITTSVIKGTDEHTDDLMLTFLLEDPPLYGEILVNGAPAEQFTQKDLLEGSVVYAHTSGEIGLLPKGDSFNLSLSEKSQEWRMGGNTIQGVTVWVTILPVDSQAPEISVGEQFTVMEGDKGVITSMHISAEDIDSLNDDLLCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHKGVEPVEDRFVFRCSDGINFSERHFFPIVIIPTNDEKPEMFMREFIVMEGMSLVIDTPILNAADADIPPDDLTFTITRFPTHGHIMSQQVNGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGEHSLEKVALIMILPVDDETPRMAINNGLEIEIGETKVINNKILMATDLDSEDKSLAYIIRYGPGHGVLQRRKLTGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFFVSIGSVDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGVSIASFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTVEDRDTPDKLLKFTVTQVPVHGHLVFNNTKPATAFTKQDLNENLISYKHDGTESSEDSFSFTVTDGTHTDFYVFPDTVFETRRPQVMKIQILPVDNSVPQIAVNKGASVLRNLATGHLGFMITSKILKVEDRDSLHFSLKFTVTEAPRHGYLFNLDKGNHSITQFTQADIDDMKICYVLKEGANATSDVFHFAVEDGGGNKLTNQHFRLNWAWISFEKEYNLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGEHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSIEEDVGELFIPIRRTGDVSQEFMVICYTQQGTATGTVPTSVLSYSDYISRPEDHTSVIRFDKDEREKMCRIVVIDDSLYEEEETFRVLLSMPMGGRIGSEFPGAQVTILPDRDDEPSFYFGNVQYSVDESSGHVEVQVWRTGTDLSRPSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPVLEGTEKFELVLRMPVNAVLGEPSKATVFINDSVSDLPKMQFKERVYTGSESDGHIAAMIHRSGDIQYRSSVRCYTRQGSAQVMMDFEERPNTDVSIITFLPGEIEKPCVLELMDDILYEEVEELRLVLGTPQSSSPFGAAVGEQNETLIRIRDDADKTIIKFGETKFSVTEPREPGESVVVEIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVEIEVIFDGVREMREAFTVHLKPDENMIAETQVTKAIVYIEEMNSMADVTFPSVPQIVSLLMYDDTAKAKESAGPVSGYPIICVTACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNTNGNEGLELTSPIVTIAREEGLCQPRVPGVVGAEPFSAKLRYTGPEDPDYSNLIKLTVTMPHIDGMLPVISTRELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHHGFLTDATKNPEVIGESYPYQYSSPLRGNGTLRFYRNLNLEACLWEFVSYYDMSELLADCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWSNGIGSPPGAELQGSLYPTSMRIREEGRLAVNFKTQAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLVRSEPTYNQPIQQWSFVSDFAVRDYSGTYTVKLVPCTTPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDTIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPTNEEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNTNRGIGKRSVEYEYHSLVHQGKPQITTGSRKKREIRSAPPLAWDIGAENNRGTNIQHIALDRANKRQTPPGRVPPEGILPRELNSPSSEVSLVTIVGSIAVGLLTICLTVIAAMMCKSKKSVRGKDTPKGSGSSEPMMPPQSHHSDSSEV

Target ORF information:

RefSeq Version	XM_005330232.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005330232.2

ORF Insert Sequence:

ATGCACCCAG GCAGAACCCC AGAGGTCGCT ACCCGCGCGC GCCGGACAGC CAACTCCGCC 
AGCTCTCAGC CAAAACTTGT TCCGCCACCC AGTCTGCTGC TGCTTCTCCT ATCACTGGTA 
AGCCGCATCT CGGCACAGCT CGCCGTGGGT GACCGAGCAT TGCTGTCTCC GGGTCTCGCG 
GGGGCTGCGG GGGTTCCTTC CGAGGAGGCC ATAGTGATGG CGAATCGCGG ACTCAGGGTG 
CCTTTCGGGC GTGAAGTGTG GCTCGATCCG CTGCACGACC TAGTGTTGCA GGTGCAGCCA 
GGGGACCGCT GCGCGGTGAC TGTGCTAGAC AACGACGCGC TGGCTCAGAG GCCCGGCCGC 
CTGAGTCCCA AGCGCTTCCC CTGCGACTTT GGCCCAGGAG AGGTGCGCTA TTCCCACCTG 
GGTGCGCGCA GCCCGTCGCG GGACCGCGTG CGACTGCAGC TGCGCTATGA CGCTCCTGGA 
GGGGCAGTAG TGCTACCACT GGTCCTGGAG GTGGAAGTAG TCTTCACCCA ACTGGAGATT 
GTGACTCGGA ACTTGCCTCT GGTCGTGGAA GAACTGCTGG GGACCAGCAA TGCCCTGGAC 
GCGCGGAGCT TGGAGTTTGC CTACCAGTCG GAGACCGAGG AGTGTCGCGT GGGCATCCTG 
ACGGGCTTGA GTGCGCTGCC TCGCTATGGA GAACTCCTCC ACTATCCGCA GGTCCCTGGA 
GAAGCCAGAG AAGGAAGTAC CTCGGAGCCT CTCTTGATGG ACTGCAAAGC TTTTCAGGAG 
CTAGGTGTGC GCTACCGTCA CACAGCCCCC AGTCGCTCAC CCAACAGAGA TTGGGTACCC 
ATGGTGGTGG AGCTGCGTTC ACGAGGGGCT CCTCTTGGCA GCCCTGCTCT AAAACGCGAG 
CACTTTCAGG TGCTAGTGAG GATCCGAGGA GGGGCTGAGA ACACCGCACC TAAGCCCAGT 
TTTGTGGCCA TGATGATGAT GGAGGTGGAC CAGTTTGTAC TGACCGCCCT GACTCCAGAC 
ATGCTGGCAG CTGAGGATGC TGAGTCTACC CCTGACCTAT TGATCTTCAA CCTCACTGCT 
CCCTTACAGC CCGGCCAGGG TTACTTGGTG AGCACCGATG ACCGCAGCCT ACCCCTCTCC 
TCCTTCACTC AGAGAGATCT GCACCTTCTG AAGATAGCCT ACCAGCCCCC TTCTGAAGAC 
TCTAACCAGG AGCGACTCTT TGAACTGGAG CTAGAAGTAG TGGACCCAGA AGGAGCAGCC 
TCCGATCCTT TTGCTTTCAT GGTAGTGGTG AAGCCTATGA ACACACTGGC TCCGGTGGTC 
ACACGAAACA CTGGTCTTAT TCTGTACGAA GGTCAGTCTC GGCCTCTCAC AGGTCCTGTA 
GGTAGTGGAC CACAAAACTT GGTCATCAGT GATGAGGATG ATCTAGAAGC AGTGCGACTG 
GAGGTGGTGG CTGGGCTCCG GCATGGTCAC CTTGTCATTC TGGGTGCTCC CAGCGGCAAC 
TCTGCTCCTA AGACTTTTAC AGTGGCAGAG CTAGCAGCTG GCCATGTGGT TTACCAGCAT 
GATGACAAAG ATGGTTCACT GAGTGACAAC CTGGTGCTCC GCATGGTGGA TGGAGGAGGC 
AGACACCAGG TGCAGTTCCT GTTCCCCATC ACTTTAGTGC CTGTGGATGA CCAGCCACCT 
GTCCTGAATG CCAACACTGG ACTGACATTG GCAGAAGGTG AAACAGTGCC TATCCCACCG 
ATTGCTCTGA GTGCAACTGA CATAGATTCT GATGACTCTC TGCTGCTTTT TGTGCTGGAG 
TCACCCTCCT CAACTGCAGG GCATCTGCTT CTCCGCCAAA CTCATCCTCC CCAAGAGGAG 
CAGGAGCTGA TCAGTGGCCT TTGGAGGAAG CAGGGGGCAT ATTATGAACG AACAGTGACA 
GAGTGGCAGC AGAAAGACAT AACAGAAGGG CGGCTCTTCT ATAGACACTC TGGGCCCCAT 
AGTCCTGGCC CAGTGATGGA CCAGTTTACA TTTAGAGTTC AGGATAACCA TGACCCCCCT 
AATCAATCTG GACTACAGAA GTTTGTCATA CGTATTCATC CTGTGGATCG CCTCCCTCCA 
GAGCTGGTCA GTGGCTGTCC CCTTCGGATG GTGGTGCAGG AATCCCAGCT CACACCACTG 
AGGAAGAAGT GGCTGCGCTA CACTGATCTG GACACTGATG ATAGAGAACT CCGTTACACC 
GTGACCCAGC CCCCTGTGGA CACCGATGAA AACCACTCAC CTGTCCCACT GGGCACCTTG 
GTCCTGACTG ATTACCCATC CACTGTGGTG ACCCATTTTA CCCAAGCCCA GATCAATCAC 
CATAAAATTG CATACAGACC CCCAGGTCAA GAACTGGGAG TGGCTGCTCG AGTGGCCCAG 
TTCCAGTTCC AGGTGGAGGA CCGAGCTGGG AATGTGGCTC CTGGTACCTT CACCCTTTAC 
TTGCAGCCCG TGGACAACCA GCCACCTGAT ATCCTCAACA CTGGCTTTAC TATTCAGGAG 
AAGGGCCACC ACATCCTAAG TGAGACTGAG TTGCATGTGA GTGATGTAGA CACTGATATG 
GCCCACATCA TTTTTACCCT CACACAGGCA CCCAAACATG GCCACATGCA AGTGTCCGGA 
CAGACACTGC ATGTAGGAGA GCTCTTCCAC TTGGAGGACA TAAAACAGGG GCGAATTTCC 
TATACTCATA ATGGAGATGA GTCCTTGACT GATAGCTGCT CCTTGGAGGT CAGTGACAGA 
CACCATGTGG TGCCTATCAC TCTCAGAGTA AATGTCCGGC CAGTGGACCA GGAGGTGCCC 
ATGCTGAGCC TTCCTACAGG TACTCTGGAG TCCTATCTAG ACGTCTTAGA AAACGGAGCT 
ACTGAAATCA CCACCAGTGT TATTAAGGGG ACTGATGAAC ACACTGATGA TTTGATGCTG 
ACTTTCCTCT TGGAAGATCC ACCCTTGTAT GGAGAAATCC TGGTCAATGG AGCTCCAGCA 
GAGCAGTTCA CCCAAAAGGA CCTCTTGGAG GGCTCCGTTG TCTATGCCCA CACCAGTGGT 
GAGATTGGCC TGTTGCCCAA AGGAGACTCT TTCAACCTGA GCCTGTCAGA AAAGTCTCAA 
GAATGGAGAA TGGGTGGCAA TACTATTCAA GGTGTCACGG TGTGGGTAAC CATCCTCCCT 
GTGGACAGCC AGGCCCCAGA AATTTCTGTG GGTGAACAGT TTACGGTAAT GGAAGGTGAT 
AAAGGTGTTA TAACCTCGAT GCACATCAGC GCTGAAGATA TTGACTCCCT GAATGATGAC 
CTCCTGTGCA CAATCGTTAT TCAGCCAACC TCAGGCTATG TGGAAAACAT TTCTCCAGCC 
CCAGGCTCTG AGAAGTCCAG GGCAGGGATT GCCATAAGTG CCTTCACTCT GAAAGATCTC 
AGGCAGGGCC ACATTAACTA TGTCCAGAGT GTCCACAAAG GGGTGGAACC TGTGGAGGAC 
CGGTTTGTAT TTCGTTGCTC TGATGGCATT AACTTTTCCG AGAGACATTT TTTCCCCATT 
GTAATCATCC CTACCAATGA TGAAAAGCCA GAAATGTTTA TGAGAGAATT TATAGTCATG 
GAAGGTATGA GTCTGGTGAT TGATACGCCC ATCCTCAATG CTGCTGATGC TGACATCCCC 
CCGGATGACT TAACCTTCAC TATTACCCGA TTTCCCACTC ATGGGCACAT CATGAGCCAG 
CAGGTAAATG GCACGGTTCT TGTCGAAAGC TTCACCTTGG ATCAGATCAT AGAGAGTTCC 
AGCATTATTT ATGAGCATGA TGACTCCGAG ACCCAGGAAG ACAGTTTTGT GATCAAGTTA 
ACGGATGGTG AGCACTCTCT GGAAAAGGTG GCCCTCATCA TGATCCTCCC TGTGGATGAC 
GAGACCCCCA GGATGGCCAT CAATAATGGA CTGGAGATAG AAATTGGGGA AACCAAAGTC 
ATCAACAACA AAATATTAAT GGCCACTGAT TTGGACTCTG AAGACAAATC CTTGGCTTAT 
ATCATTCGTT ATGGGCCAGG ACATGGTGTA TTACAGAGGC GAAAACTTAC AGGTGCCTTT 
GAAAATATCA CGCTGGGCAT GAATTTTACC CAGGATGAAG TGGACAGAAA CTTAATTCAG 
TATGTCCATT TTGGGCAAGA GGGCATCCGG GACCTAATTA AATTTGATGT GACTGATGGA 
ATAAACGCCC TCATTGATCG CTACTTTTTT GTGTCCATTG GGAGTGTTGA CATTGTCTTC 
CCTGATGTGA TTAGTAAGGG GGTGTCCTTG AAGGAAGGTG GTAAAGTCAC CCTCACAACA 
GACCTACTAA GCACAAGTGA CTTGAACAGC CCCGATGAAA ACTTGGTTTT TACCATCACG 
AGGGCCCCCA TGAGAGGTCA CCTAGAGTGC ACTGACCAAC CTGGAGTGTC CATCGCATCT 
TTCACTCAGC TCCAATTGGC TGGCAACAAA ATCTACTACA TCCACACAGC TGACGATGAG 
GTGAAGATGG ACAGCTTTGA GTTTCAAGTC ACTGACGGGC GTAACCCTGT CTTCCGAACG 
TTCCGTATCT CCATTAGTGA CGTGGACAAC AAAAAGCCAG TGGTCACCAT CCATAACTTG 
GTTGTCAGCG AAGGTGAAAA CAAGCTGATC ACGCCCTTTG AACTCACTGT TGAAGACAGA 
GATACTCCCG ACAAACTCCT GAAATTCACT GTCACCCAGG TGCCTGTTCA TGGTCATCTC 
GTTTTCAATA ATACCAAACC TGCCACGGCT TTTACCAAGC AAGACTTGAA TGAAAACTTA 
ATCAGCTACA AGCATGATGG CACGGAGTCG AGTGAAGACA GCTTCTCCTT CACGGTGACC 
GATGGCACCC ATACAGACTT TTATGTTTTC CCAGATACAG TGTTTGAAAC GAGGAGACCT 
CAAGTGATGA AGATCCAGAT CTTACCTGTT GACAATAGTG TCCCCCAAAT TGCAGTGAAT 
AAAGGGGCCT CTGTGCTTCG CAATCTGGCC ACTGGTCACT TGGGGTTCAT GATCACAAGC 
AAAATATTGA AAGTGGAGGA TAGAGACAGC CTGCACTTCT CTCTGAAGTT TACTGTGACA 
GAGGCACCTC GACATGGATA TCTCTTCAAC CTGGATAAAG GCAACCACAG CATCACTCAG 
TTTACTCAAG CTGACATTGA TGACATGAAA ATATGCTATG TCTTAAAAGA AGGGGCTAAT 
GCCACAAGTG ATGTGTTCCA TTTTGCAGTT GAAGATGGTG GTGGAAACAA GTTAACCAAT 
CAGCATTTTC GTCTGAATTG GGCATGGATC TCCTTCGAAA AGGAATATAA CCTGATCAAT 
GAGGACTCCA AATTTCTCGA TGTTGTTCTT AAACGTAGAG GTTACTTGGG AGAAACTTCA 
TTTATAAGTA TTGGCACAAG AGATGGGACG GCAGAAAAGG ACAAGGACTT TAAAGGCAAA 
GCACAGAAGC AAGTGCAGTT CAACCCTGGC CAGACCAGGG CCACGTGGAG AGTGCGTATC 
CTGAGTGACG GGGAACATGA GCAATCCGAG ACCTTCCAGG TGGTTCTCTC GGAGCCTGTG 
CTGGCTGCCC TGGAGTTCCC TGCAGTGACC ACGGTAGAAA TTGTTGACCC AGGAGATGAA 
TCAACTGTGT TCATTCCCCA GTCGGAATAC TCCATTGAAG AAGATGTGGG CGAGCTGTTC 
ATTCCCATCA GGAGGACAGG AGACGTCAGC CAGGAGTTCA TGGTGATCTG TTACACACAG 
CAAGGAACAG CGACCGGAAC TGTGCCAACT TCCGTGTTGT CTTATTCGGA TTACATATCC 
AGGCCCGAGG ACCATACCAG TGTTATTCGC TTTGACAAAG ATGAAAGGGA GAAAATGTGT 
CGGATAGTTG TGATTGATGA CTCCTTATAT GAAGAAGAGG AAACGTTCCG TGTCCTTTTG 
AGCATGCCCA TGGGTGGGAG AATTGGATCA GAGTTCCCAG GAGCTCAGGT TACAATCCTC 
CCCGACAGAG ATGATGAACC CAGCTTCTAC TTTGGGAATG TGCAGTACTC TGTGGACGAG 
AGCTCCGGCC ACGTAGAGGT GCAGGTGTGG AGAACAGGTA CCGACCTCTC CAGGCCTTCC 
AGTGTCACAG TGAGATCTCG GAAGACGGAT CCTCCTTCTG CAGATGCTGG GACAGACTAT 
GTGGGAATCA GTCGCAATTT AGATTTTGCA CCTGGAGTCA ACATGCAGAC TGTTCGTGTT 
ATCATTCTGG ATGACCTTGG ACAACCAGTG CTGGAGGGAA CTGAGAAATT TGAACTGGTG 
CTTCGTATGC CTGTGAATGC TGTCCTTGGG GAGCCCAGCA AAGCCACGGT GTTCATAAAT 
GACTCTGTCT CAGACTTGCC TAAGATGCAA TTCAAAGAAC GAGTTTACAC TGGCAGCGAA 
AGTGATGGGC ATATAGCTGC TATGATCCAC AGGAGTGGTG ATATCCAGTA CAGGTCTTCA 
GTGCGATGCT ATACACGGCA GGGGTCCGCA CAGGTGATGA TGGACTTTGA AGAACGCCCA 
AACACTGACG TCTCCATCAT CACATTCCTC CCTGGTGAGA TTGAAAAGCC ATGTGTCCTT 
GAGCTGATGG ATGACATACT CTACGAGGAG GTGGAAGAGC TCCGCCTGGT ACTTGGCACT 
CCGCAAAGCA GCTCTCCCTT TGGGGCTGCC GTTGGTGAAC AGAATGAAAC TCTGATAAGG 
ATCCGAGACG ATGCTGATAA GACTATTATC AAATTTGGAG AAACCAAATT TAGCGTCACC 
GAACCCAGAG AACCAGGAGA GTCAGTCGTG GTTGAAATTC CAGTGATTCG CCAAGGAGAC 
ACTTCGAAGG TCTCCATTGT GAGAGTCCAC ACCAAGGATG GCTCTGCCAC CTCCGGAGAA 
GACTATCACC CTGTGTCAGA AGAAATTGAG TTTAAGGAAG GAGAAACCCA GCACATTGTT 
GAAATTGAAG TAATCTTCGA TGGGGTGAGA GAGATGAGAG AGGCCTTCAC TGTTCACTTA 
AAACCTGATG AAAATATGAT AGCAGAGACC CAGGTGACCA AGGCCATTGT GTATATAGAA 
GAAATGAACA GCATGGCAGA TGTCACTTTT CCTTCTGTCC CTCAAATTGT GTCCTTGCTG 
ATGTATGACG ACACGGCCAA AGCTAAGGAG AGTGCAGGAC CTGTGTCGGG CTATCCCATC 
ATCTGCGTCA CAGCTTGCAA CCCCAAATAT TCAGACTACG ATAAAACGGG CTCTATTTGT 
GCAAGTGAGA ACATCAACGA CACTCTGACC CGCTACCGAT GGCTGATTAG CGCTCCTGCT 
GGCCCTGATG GTGTGACCAG CCCCATGAGA GAGGTGGACT TCGATACCTT TTTTACATCA 
TCCAAGATGA TCACCTTAGA CTCCATATAC TTTCAGCCTG GCTCTCGGGT GCAGTGTGCG 
GCTCGTGCTG TGAACACCAA CGGGAATGAA GGTCTGGAGC TGACAAGCCC TATCGTTACC 
ATTGCCAGGG AAGAAGGTCT TTGTCAACCC CGTGTGCCTG GAGTAGTGGG GGCTGAACCG 
TTCTCAGCTA AATTGCGCTA CACAGGGCCC GAGGACCCGG ACTACTCAAA CCTTATCAAG 
CTCACAGTCA CCATGCCACA CATAGATGGC ATGCTCCCGG TGATCTCCAC CAGAGAGCTC 
TCCAACTTCG AGCTGACCCT CAGCCCCGAT GGCACCAGAG TTGGGAACCA CAAGTGCTCC 
AACCTCCTGG ACTACACTGA GGTGAAGACC CACCATGGCT TCCTGACGGA TGCCACGAAG 
AATCCAGAAG TCATTGGAGA GTCGTATCCT TACCAGTACA GCTCACCTCT CAGAGGAAAC 
GGCACCTTAC GCTTCTACCG GAACCTCAAC CTGGAGGCCT GTTTGTGGGA GTTTGTCAGC 
TACTATGACA TGTCAGAGCT CCTCGCTGAC TGCGGAGGCA CCATTGGAAC GGACGGACAG 
GTCCTAAACC TAGTGCAGTC TTACGTGACC CTTCGAGTAC CTCTGTATGT GTCCTACGTG 
TTCCACTCCC CAGTGGGAGT AGGTGGCTGG CAGCATTTTG ATTTGAAGTC GGAGCTTCGT 
CTGACTTTCG TGTATGACAC TGCCATCTTG TGGAGCAATG GAATTGGCAG CCCACCAGGA 
GCCGAACTCC AAGGATCTCT CTACCCAACG AGCATGCGCA TCAGGGAAGA GGGGCGCCTG 
GCTGTGAACT TCAAGACCCA GGCTCAGTTC CATGGCTTGT TTGTGCTGTC ACATCCCGCA 
TCTTTTACCA GCTCAATGAT CATGTCAGCT GATCATCCAG GCCTGACCTT TTCCCTCCGC 
CTTGTAAGGA GTGAACCAAC CTATAACCAG CCTATTCAGC AATGGAGCTT TGTCTCTGAC 
TTTGCAGTAC GTGACTACTC GGGAACCTAT ACTGTAAAGC TGGTGCCATG CACCACCCCG 
TCCAATCAGG AGTACCGACT GCCAGTCACC TGCAACCCCA GAGAGCCTGT CACCTTTGAC 
CTTGACATCC GATTCCAACA GGTCAGCGAT CCAGTGGCAG CTGAATTCAG CCTCAATACT 
CAGATGTACC TACTCTCCAA GAAGAGTCTC TGGTTGTCTG ACGGATCCAT GGGATTTGGA 
CAAGAAAGCG ATGTTGCTTT TGCAGAAGGT GATACAATTT ATGGTCGTGT CATGGTAGAT 
CCTGTCCAGA ATCTGGGTGA CTCCTTTTAC TGCAGCATTG AGAAGGTTTT CCTATGCACT 
GGAGCTGATG GCTATGTCCC CAAGTATAGT CCAACAAATG AAGAATATGG CTGTTTAGCC 
GACTCTCCTT CACTTCTGTA TAGATTTAAA ATTGTGGACA AAGCTCAACC AGAGACACAA 
GCCACCAGTT TTGGAAATGT CCTCTTTAAT GCCAAACTGG CAGTGGATGA CCCTGAAGCC 
ATCCTCTTAG TGAATCAGCC TGGCTCTGAT GGATTTAAAG TCGACTCAAC ACCACTCTTT 
CAGGTTGCTC TGGGCCGAGA ATGGTACATA CACACAATCT ATACAGTAAG ATCAAAAGAC 
AACACCAATC GAGGTATCGG CAAAAGAAGT GTGGAGTATG AGTACCACTC TCTGGTGCAT 
CAAGGGAAGC CACAGATAAC TACCGGGAGC CGGAAGAAGA GAGAGATCAG GAGCGCACCC 
CCACTGGCAT GGGACATCGG TGCTGAAAAC AACCGAGGGA CAAACATCCA GCACATTGCC 
CTGGACCGCG CCAACAAGAG GCAGACCCCC CCAGGGAGAG TGCCCCCCGA AGGCATCCTA 
CCCCGGGAGC TCAACAGTCC CAGCTCTGAG GTCAGTCTGG TCACCATCGT GGGCAGCATC 
GCGGTGGGAT TACTCACCAT CTGCCTCACT GTCATTGCAG CAATGATGTG CAAAAGCAAG 
AAAAGTGTCA GGGGTAAGGA CACTCCGAAG GGCTCTGGCA GCAGCGAGCC CATGATGCCC 
CCGCAGAGCC ACCATAGCGA CAGCTCAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem2 ( XM_005330232.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005330289.1 Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.

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CloneID	OIc61233
Clone ID Related Accession (Same CDS sequence)	XM_005330232.2 , XM_005330232.3
Accession Version	XM_005330232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	FRAS1-related extracellular matrix protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936565.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005330232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCAG GCAGAACCCC AGAGGTCGCT ACCCGCGCGC GCCGGACAGC CAACTCCGCC 
AGCTCTCAGC CAAAACTTGT TCCGCCACCC AGTCTGCTGC TGCTTCTCCT ATCACTGGTA 
AGCCGCATCT CGGCACAGCT CGCCGTGGGT GACCGAGCAT TGCTGTCTCC GGGTCTCGCG 
GGGGCTGCGG GGGTTCCTTC CGAGGAGGCC ATAGTGATGG CGAATCGCGG ACTCAGGGTG 
CCTTTCGGGC GTGAAGTGTG GCTCGATCCG CTGCACGACC TAGTGTTGCA GGTGCAGCCA 
GGGGACCGCT GCGCGGTGAC TGTGCTAGAC AACGACGCGC TGGCTCAGAG GCCCGGCCGC 
CTGAGTCCCA AGCGCTTCCC CTGCGACTTT GGCCCAGGAG AGGTGCGCTA TTCCCACCTG 
GGTGCGCGCA GCCCGTCGCG GGACCGCGTG CGACTGCAGC TGCGCTATGA CGCTCCTGGA 
GGGGCAGTAG TGCTACCACT GGTCCTGGAG GTGGAAGTAG TCTTCACCCA ACTGGAGATT 
GTGACTCGGA ACTTGCCTCT GGTCGTGGAA GAACTGCTGG GGACCAGCAA TGCCCTGGAC 
GCGCGGAGCT TGGAGTTTGC CTACCAGTCG GAGACCGAGG AGTGTCGCGT GGGCATCCTG 
ACGGGCTTGA GTGCGCTGCC TCGCTATGGA GAACTCCTCC ACTATCCGCA GGTCCCTGGA 
GAAGCCAGAG AAGGAAGTAC CTCGGAGCCT CTCTTGATGG ACTGCAAAGC TTTTCAGGAG 
CTAGGTGTGC GCTACCGTCA CACAGCCCCC AGTCGCTCAC CCAACAGAGA TTGGGTACCC 
ATGGTGGTGG AGCTGCGTTC ACGAGGGGCT CCTCTTGGCA GCCCTGCTCT AAAACGCGAG 
CACTTTCAGG TGCTAGTGAG GATCCGAGGA GGGGCTGAGA ACACCGCACC TAAGCCCAGT 
TTTGTGGCCA TGATGATGAT GGAGGTGGAC CAGTTTGTAC TGACCGCCCT GACTCCAGAC 
ATGCTGGCAG CTGAGGATGC TGAGTCTACC CCTGACCTAT TGATCTTCAA CCTCACTGCT 
CCCTTACAGC CCGGCCAGGG TTACTTGGTG AGCACCGATG ACCGCAGCCT ACCCCTCTCC 
TCCTTCACTC AGAGAGATCT GCACCTTCTG AAGATAGCCT ACCAGCCCCC TTCTGAAGAC 
TCTAACCAGG AGCGACTCTT TGAACTGGAG CTAGAAGTAG TGGACCCAGA AGGAGCAGCC 
TCCGATCCTT TTGCTTTCAT GGTAGTGGTG AAGCCTATGA ACACACTGGC TCCGGTGGTC 
ACACGAAACA CTGGTCTTAT TCTGTACGAA GGTCAGTCTC GGCCTCTCAC AGGTCCTGTA 
GGTAGTGGAC CACAAAACTT GGTCATCAGT GATGAGGATG ATCTAGAAGC AGTGCGACTG 
GAGGTGGTGG CTGGGCTCCG GCATGGTCAC CTTGTCATTC TGGGTGCTCC CAGCGGCAAC 
TCTGCTCCTA AGACTTTTAC AGTGGCAGAG CTAGCAGCTG GCCATGTGGT TTACCAGCAT 
GATGACAAAG ATGGTTCACT GAGTGACAAC CTGGTGCTCC GCATGGTGGA TGGAGGAGGC 
AGACACCAGG TGCAGTTCCT GTTCCCCATC ACTTTAGTGC CTGTGGATGA CCAGCCACCT 
GTCCTGAATG CCAACACTGG ACTGACATTG GCAGAAGGTG AAACAGTGCC TATCCCACCG 
ATTGCTCTGA GTGCAACTGA CATAGATTCT GATGACTCTC TGCTGCTTTT TGTGCTGGAG 
TCACCCTCCT CAACTGCAGG GCATCTGCTT CTCCGCCAAA CTCATCCTCC CCAAGAGGAG 
CAGGAGCTGA TCAGTGGCCT TTGGAGGAAG CAGGGGGCAT ATTATGAACG AACAGTGACA 
GAGTGGCAGC AGAAAGACAT AACAGAAGGG CGGCTCTTCT ATAGACACTC TGGGCCCCAT 
AGTCCTGGCC CAGTGATGGA CCAGTTTACA TTTAGAGTTC AGGATAACCA TGACCCCCCT 
AATCAATCTG GACTACAGAA GTTTGTCATA CGTATTCATC CTGTGGATCG CCTCCCTCCA 
GAGCTGGTCA GTGGCTGTCC CCTTCGGATG GTGGTGCAGG AATCCCAGCT CACACCACTG 
AGGAAGAAGT GGCTGCGCTA CACTGATCTG GACACTGATG ATAGAGAACT CCGTTACACC 
GTGACCCAGC CCCCTGTGGA CACCGATGAA AACCACTCAC CTGTCCCACT GGGCACCTTG 
GTCCTGACTG ATTACCCATC CACTGTGGTG ACCCATTTTA CCCAAGCCCA GATCAATCAC 
CATAAAATTG CATACAGACC CCCAGGTCAA GAACTGGGAG TGGCTGCTCG AGTGGCCCAG 
TTCCAGTTCC AGGTGGAGGA CCGAGCTGGG AATGTGGCTC CTGGTACCTT CACCCTTTAC 
TTGCAGCCCG TGGACAACCA GCCACCTGAT ATCCTCAACA CTGGCTTTAC TATTCAGGAG 
AAGGGCCACC ACATCCTAAG TGAGACTGAG TTGCATGTGA GTGATGTAGA CACTGATATG 
GCCCACATCA TTTTTACCCT CACACAGGCA CCCAAACATG GCCACATGCA AGTGTCCGGA 
CAGACACTGC ATGTAGGAGA GCTCTTCCAC TTGGAGGACA TAAAACAGGG GCGAATTTCC 
TATACTCATA ATGGAGATGA GTCCTTGACT GATAGCTGCT CCTTGGAGGT CAGTGACAGA 
CACCATGTGG TGCCTATCAC TCTCAGAGTA AATGTCCGGC CAGTGGACCA GGAGGTGCCC 
ATGCTGAGCC TTCCTACAGG TACTCTGGAG TCCTATCTAG ACGTCTTAGA AAACGGAGCT 
ACTGAAATCA CCACCAGTGT TATTAAGGGG ACTGATGAAC ACACTGATGA TTTGATGCTG 
ACTTTCCTCT TGGAAGATCC ACCCTTGTAT GGAGAAATCC TGGTCAATGG AGCTCCAGCA 
GAGCAGTTCA CCCAAAAGGA CCTCTTGGAG GGCTCCGTTG TCTATGCCCA CACCAGTGGT 
GAGATTGGCC TGTTGCCCAA AGGAGACTCT TTCAACCTGA GCCTGTCAGA AAAGTCTCAA 
GAATGGAGAA TGGGTGGCAA TACTATTCAA GGTGTCACGG TGTGGGTAAC CATCCTCCCT 
GTGGACAGCC AGGCCCCAGA AATTTCTGTG GGTGAACAGT TTACGGTAAT GGAAGGTGAT 
AAAGGTGTTA TAACCTCGAT GCACATCAGC GCTGAAGATA TTGACTCCCT GAATGATGAC 
CTCCTGTGCA CAATCGTTAT TCAGCCAACC TCAGGCTATG TGGAAAACAT TTCTCCAGCC 
CCAGGCTCTG AGAAGTCCAG GGCAGGGATT GCCATAAGTG CCTTCACTCT GAAAGATCTC 
AGGCAGGGCC ACATTAACTA TGTCCAGAGT GTCCACAAAG GGGTGGAACC TGTGGAGGAC 
CGGTTTGTAT TTCGTTGCTC TGATGGCATT AACTTTTCCG AGAGACATTT TTTCCCCATT 
GTAATCATCC CTACCAATGA TGAAAAGCCA GAAATGTTTA TGAGAGAATT TATAGTCATG 
GAAGGTATGA GTCTGGTGAT TGATACGCCC ATCCTCAATG CTGCTGATGC TGACATCCCC 
CCGGATGACT TAACCTTCAC TATTACCCGA TTTCCCACTC ATGGGCACAT CATGAGCCAG 
CAGGTAAATG GCACGGTTCT TGTCGAAAGC TTCACCTTGG ATCAGATCAT AGAGAGTTCC 
AGCATTATTT ATGAGCATGA TGACTCCGAG ACCCAGGAAG ACAGTTTTGT GATCAAGTTA 
ACGGATGGTG AGCACTCTCT GGAAAAGGTG GCCCTCATCA TGATCCTCCC TGTGGATGAC 
GAGACCCCCA GGATGGCCAT CAATAATGGA CTGGAGATAG AAATTGGGGA AACCAAAGTC 
ATCAACAACA AAATATTAAT GGCCACTGAT TTGGACTCTG AAGACAAATC CTTGGCTTAT 
ATCATTCGTT ATGGGCCAGG ACATGGTGTA TTACAGAGGC GAAAACTTAC AGGTGCCTTT 
GAAAATATCA CGCTGGGCAT GAATTTTACC CAGGATGAAG TGGACAGAAA CTTAATTCAG 
TATGTCCATT TTGGGCAAGA GGGCATCCGG GACCTAATTA AATTTGATGT GACTGATGGA 
ATAAACGCCC TCATTGATCG CTACTTTTTT GTGTCCATTG GGAGTGTTGA CATTGTCTTC 
CCTGATGTGA TTAGTAAGGG GGTGTCCTTG AAGGAAGGTG GTAAAGTCAC CCTCACAACA 
GACCTACTAA GCACAAGTGA CTTGAACAGC CCCGATGAAA ACTTGGTTTT TACCATCACG 
AGGGCCCCCA TGAGAGGTCA CCTAGAGTGC ACTGACCAAC CTGGAGTGTC CATCGCATCT 
TTCACTCAGC TCCAATTGGC TGGCAACAAA ATCTACTACA TCCACACAGC TGACGATGAG 
GTGAAGATGG ACAGCTTTGA GTTTCAAGTC ACTGACGGGC GTAACCCTGT CTTCCGAACG 
TTCCGTATCT CCATTAGTGA CGTGGACAAC AAAAAGCCAG TGGTCACCAT CCATAACTTG 
GTTGTCAGCG AAGGTGAAAA CAAGCTGATC ACGCCCTTTG AACTCACTGT TGAAGACAGA 
GATACTCCCG ACAAACTCCT GAAATTCACT GTCACCCAGG TGCCTGTTCA TGGTCATCTC 
GTTTTCAATA ATACCAAACC TGCCACGGCT TTTACCAAGC AAGACTTGAA TGAAAACTTA 
ATCAGCTACA AGCATGATGG CACGGAGTCG AGTGAAGACA GCTTCTCCTT CACGGTGACC 
GATGGCACCC ATACAGACTT TTATGTTTTC CCAGATACAG TGTTTGAAAC GAGGAGACCT 
CAAGTGATGA AGATCCAGAT CTTACCTGTT GACAATAGTG TCCCCCAAAT TGCAGTGAAT 
AAAGGGGCCT CTGTGCTTCG CAATCTGGCC ACTGGTCACT TGGGGTTCAT GATCACAAGC 
AAAATATTGA AAGTGGAGGA TAGAGACAGC CTGCACTTCT CTCTGAAGTT TACTGTGACA 
GAGGCACCTC GACATGGATA TCTCTTCAAC CTGGATAAAG GCAACCACAG CATCACTCAG 
TTTACTCAAG CTGACATTGA TGACATGAAA ATATGCTATG TCTTAAAAGA AGGGGCTAAT 
GCCACAAGTG ATGTGTTCCA TTTTGCAGTT GAAGATGGTG GTGGAAACAA GTTAACCAAT 
CAGCATTTTC GTCTGAATTG GGCATGGATC TCCTTCGAAA AGGAATATAA CCTGATCAAT 
GAGGACTCCA AATTTCTCGA TGTTGTTCTT AAACGTAGAG GTTACTTGGG AGAAACTTCA 
TTTATAAGTA TTGGCACAAG AGATGGGACG GCAGAAAAGG ACAAGGACTT TAAAGGCAAA 
GCACAGAAGC AAGTGCAGTT CAACCCTGGC CAGACCAGGG CCACGTGGAG AGTGCGTATC 
CTGAGTGACG GGGAACATGA GCAATCCGAG ACCTTCCAGG TGGTTCTCTC GGAGCCTGTG 
CTGGCTGCCC TGGAGTTCCC TGCAGTGACC ACGGTAGAAA TTGTTGACCC AGGAGATGAA 
TCAACTGTGT TCATTCCCCA GTCGGAATAC TCCATTGAAG AAGATGTGGG CGAGCTGTTC 
ATTCCCATCA GGAGGACAGG AGACGTCAGC CAGGAGTTCA TGGTGATCTG TTACACACAG 
CAAGGAACAG CGACCGGAAC TGTGCCAACT TCCGTGTTGT CTTATTCGGA TTACATATCC 
AGGCCCGAGG ACCATACCAG TGTTATTCGC TTTGACAAAG ATGAAAGGGA GAAAATGTGT 
CGGATAGTTG TGATTGATGA CTCCTTATAT GAAGAAGAGG AAACGTTCCG TGTCCTTTTG 
AGCATGCCCA TGGGTGGGAG AATTGGATCA GAGTTCCCAG GAGCTCAGGT TACAATCCTC 
CCCGACAGAG ATGATGAACC CAGCTTCTAC TTTGGGAATG TGCAGTACTC TGTGGACGAG 
AGCTCCGGCC ACGTAGAGGT GCAGGTGTGG AGAACAGGTA CCGACCTCTC CAGGCCTTCC 
AGTGTCACAG TGAGATCTCG GAAGACGGAT CCTCCTTCTG CAGATGCTGG GACAGACTAT 
GTGGGAATCA GTCGCAATTT AGATTTTGCA CCTGGAGTCA ACATGCAGAC TGTTCGTGTT 
ATCATTCTGG ATGACCTTGG ACAACCAGTG CTGGAGGGAA CTGAGAAATT TGAACTGGTG 
CTTCGTATGC CTGTGAATGC TGTCCTTGGG GAGCCCAGCA AAGCCACGGT GTTCATAAAT 
GACTCTGTCT CAGACTTGCC TAAGATGCAA TTCAAAGAAC GAGTTTACAC TGGCAGCGAA 
AGTGATGGGC ATATAGCTGC TATGATCCAC AGGAGTGGTG ATATCCAGTA CAGGTCTTCA 
GTGCGATGCT ATACACGGCA GGGGTCCGCA CAGGTGATGA TGGACTTTGA AGAACGCCCA 
AACACTGACG TCTCCATCAT CACATTCCTC CCTGGTGAGA TTGAAAAGCC ATGTGTCCTT 
GAGCTGATGG ATGACATACT CTACGAGGAG GTGGAAGAGC TCCGCCTGGT ACTTGGCACT 
CCGCAAAGCA GCTCTCCCTT TGGGGCTGCC GTTGGTGAAC AGAATGAAAC TCTGATAAGG 
ATCCGAGACG ATGCTGATAA GACTATTATC AAATTTGGAG AAACCAAATT TAGCGTCACC 
GAACCCAGAG AACCAGGAGA GTCAGTCGTG GTTGAAATTC CAGTGATTCG CCAAGGAGAC 
ACTTCGAAGG TCTCCATTGT GAGAGTCCAC ACCAAGGATG GCTCTGCCAC CTCCGGAGAA 
GACTATCACC CTGTGTCAGA AGAAATTGAG TTTAAGGAAG GAGAAACCCA GCACATTGTT 
GAAATTGAAG TAATCTTCGA TGGGGTGAGA GAGATGAGAG AGGCCTTCAC TGTTCACTTA 
AAACCTGATG AAAATATGAT AGCAGAGACC CAGGTGACCA AGGCCATTGT GTATATAGAA 
GAAATGAACA GCATGGCAGA TGTCACTTTT CCTTCTGTCC CTCAAATTGT GTCCTTGCTG 
ATGTATGACG ACACGGCCAA AGCTAAGGAG AGTGCAGGAC CTGTGTCGGG CTATCCCATC 
ATCTGCGTCA CAGCTTGCAA CCCCAAATAT TCAGACTACG ATAAAACGGG CTCTATTTGT 
GCAAGTGAGA ACATCAACGA CACTCTGACC CGCTACCGAT GGCTGATTAG CGCTCCTGCT 
GGCCCTGATG GTGTGACCAG CCCCATGAGA GAGGTGGACT TCGATACCTT TTTTACATCA 
TCCAAGATGA TCACCTTAGA CTCCATATAC TTTCAGCCTG GCTCTCGGGT GCAGTGTGCG 
GCTCGTGCTG TGAACACCAA CGGGAATGAA GGTCTGGAGC TGACAAGCCC TATCGTTACC 
ATTGCCAGGG AAGAAGGTCT TTGTCAACCC CGTGTGCCTG GAGTAGTGGG GGCTGAACCG 
TTCTCAGCTA AATTGCGCTA CACAGGGCCC GAGGACCCGG ACTACTCAAA CCTTATCAAG 
CTCACAGTCA CCATGCCACA CATAGATGGC ATGCTCCCGG TGATCTCCAC CAGAGAGCTC 
TCCAACTTCG AGCTGACCCT CAGCCCCGAT GGCACCAGAG TTGGGAACCA CAAGTGCTCC 
AACCTCCTGG ACTACACTGA GGTGAAGACC CACCATGGCT TCCTGACGGA TGCCACGAAG 
AATCCAGAAG TCATTGGAGA GTCGTATCCT TACCAGTACA GCTCACCTCT CAGAGGAAAC 
GGCACCTTAC GCTTCTACCG GAACCTCAAC CTGGAGGCCT GTTTGTGGGA GTTTGTCAGC 
TACTATGACA TGTCAGAGCT CCTCGCTGAC TGCGGAGGCA CCATTGGAAC GGACGGACAG 
GTCCTAAACC TAGTGCAGTC TTACGTGACC CTTCGAGTAC CTCTGTATGT GTCCTACGTG 
TTCCACTCCC CAGTGGGAGT AGGTGGCTGG CAGCATTTTG ATTTGAAGTC GGAGCTTCGT 
CTGACTTTCG TGTATGACAC TGCCATCTTG TGGAGCAATG GAATTGGCAG CCCACCAGGA 
GCCGAACTCC AAGGATCTCT CTACCCAACG AGCATGCGCA TCAGGGAAGA GGGGCGCCTG 
GCTGTGAACT TCAAGACCCA GGCTCAGTTC CATGGCTTGT TTGTGCTGTC ACATCCCGCA 
TCTTTTACCA GCTCAATGAT CATGTCAGCT GATCATCCAG GCCTGACCTT TTCCCTCCGC 
CTTGTAAGGA GTGAACCAAC CTATAACCAG CCTATTCAGC AATGGAGCTT TGTCTCTGAC 
TTTGCAGTAC GTGACTACTC GGGAACCTAT ACTGTAAAGC TGGTGCCATG CACCACCCCG 
TCCAATCAGG AGTACCGACT GCCAGTCACC TGCAACCCCA GAGAGCCTGT CACCTTTGAC 
CTTGACATCC GATTCCAACA GGTCAGCGAT CCAGTGGCAG CTGAATTCAG CCTCAATACT 
CAGATGTACC TACTCTCCAA GAAGAGTCTC TGGTTGTCTG ACGGATCCAT GGGATTTGGA 
CAAGAAAGCG ATGTTGCTTT TGCAGAAGGT GATACAATTT ATGGTCGTGT CATGGTAGAT 
CCTGTCCAGA ATCTGGGTGA CTCCTTTTAC TGCAGCATTG AGAAGGTTTT CCTATGCACT 
GGAGCTGATG GCTATGTCCC CAAGTATAGT CCAACAAATG AAGAATATGG CTGTTTAGCC 
GACTCTCCTT CACTTCTGTA TAGATTTAAA ATTGTGGACA AAGCTCAACC AGAGACACAA 
GCCACCAGTT TTGGAAATGT CCTCTTTAAT GCCAAACTGG CAGTGGATGA CCCTGAAGCC 
ATCCTCTTAG TGAATCAGCC TGGCTCTGAT GGATTTAAAG TCGACTCAAC ACCACTCTTT 
CAGGTTGCTC TGGGCCGAGA ATGGTACATA CACACAATCT ATACAGTAAG ATCAAAAGAC 
AACACCAATC GAGGTATCGG CAAAAGAAGT GTGGAGTATG AGTACCACTC TCTGGTGCAT 
CAAGGGAAGC CACAGATAAC TACCGGGAGC CGGAAGAAGA GAGAGATCAG GAGCGCACCC 
CCACTGGCAT GGGACATCGG TGCTGAAAAC AACCGAGGGA CAAACATCCA GCACATTGCC 
CTGGACCGCG CCAACAAGAG GCAGACCCCC CCAGGGAGAG TGCCCCCCGA AGGCATCCTA 
CCCCGGGAGC TCAACAGTCC CAGCTCTGAG GTCAGTCTGG TCACCATCGT GGGCAGCATC 
GCGGTGGGAT TACTCACCAT CTGCCTCACT GTCATTGCAG CAATGATGTG CAAAAGCAAG 
AAAAGTGTCA GGGGTAAGGA CACTCCGAAG GGCTCTGGCA GCAGCGAGCC CATGATGCCC 
CCGCAGAGCC ACCATAGCGA CAGCTCAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005330289.1
CDS	1..9516
Translation	MHPGRTPEVATRARRTANSASSQPKLVPPPSLLLLLLSLVSRISAQLAVGDRALLSPGLAGAAGVPSEEAIVMANRGLRVPFGREVWLDPLHDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAVVLPLVLEVEVVFTQLEIVTRNLPLVVEELLGTSNALDARSLEFAYQSETEECRVGILTGLSALPRYGELLHYPQVPGEAREGSTSEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGAPLGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESTPDLLIFNLTAPLQPGQGYLVSTDDRSLPLSSFTQRDLHLLKIAYQPPSEDSNQERLFELELEVVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGPVGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGAPSGNSAPKTFTVAELAAGHVVYQHDDKDGSLSDNLVLRMVDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPIPPIALSATDIDSDDSLLLFVLESPSSTAGHLLLRQTHPPQEEQELISGLWRKQGAYYERTVTEWQQKDITEGRLFYRHSGPHSPGPVMDQFTFRVQDNHDPPNQSGLQKFVIRIHPVDRLPPELVSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPVDTDENHSPVPLGTLVLTDYPSTVVTHFTQAQINHHKIAYRPPGQELGVAARVAQFQFQVEDRAGNVAPGTFTLYLQPVDNQPPDILNTGFTIQEKGHHILSETELHVSDVDTDMAHIIFTLTQAPKHGHMQVSGQTLHVGELFHLEDIKQGRISYTHNGDESLTDSCSLEVSDRHHVVPITLRVNVRPVDQEVPMLSLPTGTLESYLDVLENGATEITTSVIKGTDEHTDDLMLTFLLEDPPLYGEILVNGAPAEQFTQKDLLEGSVVYAHTSGEIGLLPKGDSFNLSLSEKSQEWRMGGNTIQGVTVWVTILPVDSQAPEISVGEQFTVMEGDKGVITSMHISAEDIDSLNDDLLCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHKGVEPVEDRFVFRCSDGINFSERHFFPIVIIPTNDEKPEMFMREFIVMEGMSLVIDTPILNAADADIPPDDLTFTITRFPTHGHIMSQQVNGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGEHSLEKVALIMILPVDDETPRMAINNGLEIEIGETKVINNKILMATDLDSEDKSLAYIIRYGPGHGVLQRRKLTGAFENITLGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFFVSIGSVDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGVSIASFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTVEDRDTPDKLLKFTVTQVPVHGHLVFNNTKPATAFTKQDLNENLISYKHDGTESSEDSFSFTVTDGTHTDFYVFPDTVFETRRPQVMKIQILPVDNSVPQIAVNKGASVLRNLATGHLGFMITSKILKVEDRDSLHFSLKFTVTEAPRHGYLFNLDKGNHSITQFTQADIDDMKICYVLKEGANATSDVFHFAVEDGGGNKLTNQHFRLNWAWISFEKEYNLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGEHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSIEEDVGELFIPIRRTGDVSQEFMVICYTQQGTATGTVPTSVLSYSDYISRPEDHTSVIRFDKDEREKMCRIVVIDDSLYEEEETFRVLLSMPMGGRIGSEFPGAQVTILPDRDDEPSFYFGNVQYSVDESSGHVEVQVWRTGTDLSRPSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVIILDDLGQPVLEGTEKFELVLRMPVNAVLGEPSKATVFINDSVSDLPKMQFKERVYTGSESDGHIAAMIHRSGDIQYRSSVRCYTRQGSAQVMMDFEERPNTDVSIITFLPGEIEKPCVLELMDDILYEEVEELRLVLGTPQSSSPFGAAVGEQNETLIRIRDDADKTIIKFGETKFSVTEPREPGESVVVEIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHIVEIEVIFDGVREMREAFTVHLKPDENMIAETQVTKAIVYIEEMNSMADVTFPSVPQIVSLLMYDDTAKAKESAGPVSGYPIICVTACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNTNGNEGLELTSPIVTIAREEGLCQPRVPGVVGAEPFSAKLRYTGPEDPDYSNLIKLTVTMPHIDGMLPVISTRELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHHGFLTDATKNPEVIGESYPYQYSSPLRGNGTLRFYRNLNLEACLWEFVSYYDMSELLADCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWSNGIGSPPGAELQGSLYPTSMRIREEGRLAVNFKTQAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLVRSEPTYNQPIQQWSFVSDFAVRDYSGTYTVKLVPCTTPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDTIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPTNEEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNTNRGIGKRSVEYEYHSLVHQGKPQITTGSRKKREIRSAPPLAWDIGAENNRGTNIQHIALDRANKRQTPPGRVPPEGILPRELNSPSSEVSLVTIVGSIAVGLLTICLTVIAAMMCKSKKSVRGKDTPKGSGSSEPMMPPQSHHSDSSEV

Target ORF information:

RefSeq Version	XM_005330232.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus FRAS1 related extracellular matrix protein 2 (Frem2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005330232.3

ORF Insert Sequence:

ATGCACCCAG GCAGAACCCC AGAGGTCGCT ACCCGCGCGC GCCGGACAGC CAACTCCGCC 
AGCTCTCAGC CAAAACTTGT TCCGCCACCC AGTCTGCTGC TGCTTCTCCT ATCACTGGTA 
AGCCGCATCT CGGCACAGCT CGCCGTGGGT GACCGAGCAT TGCTGTCTCC GGGTCTCGCG 
GGGGCTGCGG GGGTTCCTTC CGAGGAGGCC ATAGTGATGG CGAATCGCGG ACTCAGGGTG 
CCTTTCGGGC GTGAAGTGTG GCTCGATCCG CTGCACGACC TAGTGTTGCA GGTGCAGCCA 
GGGGACCGCT GCGCGGTGAC TGTGCTAGAC AACGACGCGC TGGCTCAGAG GCCCGGCCGC 
CTGAGTCCCA AGCGCTTCCC CTGCGACTTT GGCCCAGGAG AGGTGCGCTA TTCCCACCTG 
GGTGCGCGCA GCCCGTCGCG GGACCGCGTG CGACTGCAGC TGCGCTATGA CGCTCCTGGA 
GGGGCAGTAG TGCTACCACT GGTCCTGGAG GTGGAAGTAG TCTTCACCCA ACTGGAGATT 
GTGACTCGGA ACTTGCCTCT GGTCGTGGAA GAACTGCTGG GGACCAGCAA TGCCCTGGAC 
GCGCGGAGCT TGGAGTTTGC CTACCAGTCG GAGACCGAGG AGTGTCGCGT GGGCATCCTG 
ACGGGCTTGA GTGCGCTGCC TCGCTATGGA GAACTCCTCC ACTATCCGCA GGTCCCTGGA 
GAAGCCAGAG AAGGAAGTAC CTCGGAGCCT CTCTTGATGG ACTGCAAAGC TTTTCAGGAG 
CTAGGTGTGC GCTACCGTCA CACAGCCCCC AGTCGCTCAC CCAACAGAGA TTGGGTACCC 
ATGGTGGTGG AGCTGCGTTC ACGAGGGGCT CCTCTTGGCA GCCCTGCTCT AAAACGCGAG 
CACTTTCAGG TGCTAGTGAG GATCCGAGGA GGGGCTGAGA ACACCGCACC TAAGCCCAGT 
TTTGTGGCCA TGATGATGAT GGAGGTGGAC CAGTTTGTAC TGACCGCCCT GACTCCAGAC 
ATGCTGGCAG CTGAGGATGC TGAGTCTACC CCTGACCTAT TGATCTTCAA CCTCACTGCT 
CCCTTACAGC CCGGCCAGGG TTACTTGGTG AGCACCGATG ACCGCAGCCT ACCCCTCTCC 
TCCTTCACTC AGAGAGATCT GCACCTTCTG AAGATAGCCT ACCAGCCCCC TTCTGAAGAC 
TCTAACCAGG AGCGACTCTT TGAACTGGAG CTAGAAGTAG TGGACCCAGA AGGAGCAGCC 
TCCGATCCTT TTGCTTTCAT GGTAGTGGTG AAGCCTATGA ACACACTGGC TCCGGTGGTC 
ACACGAAACA CTGGTCTTAT TCTGTACGAA GGTCAGTCTC GGCCTCTCAC AGGTCCTGTA 
GGTAGTGGAC CACAAAACTT GGTCATCAGT GATGAGGATG ATCTAGAAGC AGTGCGACTG 
GAGGTGGTGG CTGGGCTCCG GCATGGTCAC CTTGTCATTC TGGGTGCTCC CAGCGGCAAC 
TCTGCTCCTA AGACTTTTAC AGTGGCAGAG CTAGCAGCTG GCCATGTGGT TTACCAGCAT 
GATGACAAAG ATGGTTCACT GAGTGACAAC CTGGTGCTCC GCATGGTGGA TGGAGGAGGC 
AGACACCAGG TGCAGTTCCT GTTCCCCATC ACTTTAGTGC CTGTGGATGA CCAGCCACCT 
GTCCTGAATG CCAACACTGG ACTGACATTG GCAGAAGGTG AAACAGTGCC TATCCCACCG 
ATTGCTCTGA GTGCAACTGA CATAGATTCT GATGACTCTC TGCTGCTTTT TGTGCTGGAG 
TCACCCTCCT CAACTGCAGG GCATCTGCTT CTCCGCCAAA CTCATCCTCC CCAAGAGGAG 
CAGGAGCTGA TCAGTGGCCT TTGGAGGAAG CAGGGGGCAT ATTATGAACG AACAGTGACA 
GAGTGGCAGC AGAAAGACAT AACAGAAGGG CGGCTCTTCT ATAGACACTC TGGGCCCCAT 
AGTCCTGGCC CAGTGATGGA CCAGTTTACA TTTAGAGTTC AGGATAACCA TGACCCCCCT 
AATCAATCTG GACTACAGAA GTTTGTCATA CGTATTCATC CTGTGGATCG CCTCCCTCCA 
GAGCTGGTCA GTGGCTGTCC CCTTCGGATG GTGGTGCAGG AATCCCAGCT CACACCACTG 
AGGAAGAAGT GGCTGCGCTA CACTGATCTG GACACTGATG ATAGAGAACT CCGTTACACC 
GTGACCCAGC CCCCTGTGGA CACCGATGAA AACCACTCAC CTGTCCCACT GGGCACCTTG 
GTCCTGACTG ATTACCCATC CACTGTGGTG ACCCATTTTA CCCAAGCCCA GATCAATCAC 
CATAAAATTG CATACAGACC CCCAGGTCAA GAACTGGGAG TGGCTGCTCG AGTGGCCCAG 
TTCCAGTTCC AGGTGGAGGA CCGAGCTGGG AATGTGGCTC CTGGTACCTT CACCCTTTAC 
TTGCAGCCCG TGGACAACCA GCCACCTGAT ATCCTCAACA CTGGCTTTAC TATTCAGGAG 
AAGGGCCACC ACATCCTAAG TGAGACTGAG TTGCATGTGA GTGATGTAGA CACTGATATG 
GCCCACATCA TTTTTACCCT CACACAGGCA CCCAAACATG GCCACATGCA AGTGTCCGGA 
CAGACACTGC ATGTAGGAGA GCTCTTCCAC TTGGAGGACA TAAAACAGGG GCGAATTTCC 
TATACTCATA ATGGAGATGA GTCCTTGACT GATAGCTGCT CCTTGGAGGT CAGTGACAGA 
CACCATGTGG TGCCTATCAC TCTCAGAGTA AATGTCCGGC CAGTGGACCA GGAGGTGCCC 
ATGCTGAGCC TTCCTACAGG TACTCTGGAG TCCTATCTAG ACGTCTTAGA AAACGGAGCT 
ACTGAAATCA CCACCAGTGT TATTAAGGGG ACTGATGAAC ACACTGATGA TTTGATGCTG 
ACTTTCCTCT TGGAAGATCC ACCCTTGTAT GGAGAAATCC TGGTCAATGG AGCTCCAGCA 
GAGCAGTTCA CCCAAAAGGA CCTCTTGGAG GGCTCCGTTG TCTATGCCCA CACCAGTGGT 
GAGATTGGCC TGTTGCCCAA AGGAGACTCT TTCAACCTGA GCCTGTCAGA AAAGTCTCAA 
GAATGGAGAA TGGGTGGCAA TACTATTCAA GGTGTCACGG TGTGGGTAAC CATCCTCCCT 
GTGGACAGCC AGGCCCCAGA AATTTCTGTG GGTGAACAGT TTACGGTAAT GGAAGGTGAT 
AAAGGTGTTA TAACCTCGAT GCACATCAGC GCTGAAGATA TTGACTCCCT GAATGATGAC 
CTCCTGTGCA CAATCGTTAT TCAGCCAACC TCAGGCTATG TGGAAAACAT TTCTCCAGCC 
CCAGGCTCTG AGAAGTCCAG GGCAGGGATT GCCATAAGTG CCTTCACTCT GAAAGATCTC 
AGGCAGGGCC ACATTAACTA TGTCCAGAGT GTCCACAAAG GGGTGGAACC TGTGGAGGAC 
CGGTTTGTAT TTCGTTGCTC TGATGGCATT AACTTTTCCG AGAGACATTT TTTCCCCATT 
GTAATCATCC CTACCAATGA TGAAAAGCCA GAAATGTTTA TGAGAGAATT TATAGTCATG 
GAAGGTATGA GTCTGGTGAT TGATACGCCC ATCCTCAATG CTGCTGATGC TGACATCCCC 
CCGGATGACT TAACCTTCAC TATTACCCGA TTTCCCACTC ATGGGCACAT CATGAGCCAG 
CAGGTAAATG GCACGGTTCT TGTCGAAAGC TTCACCTTGG ATCAGATCAT AGAGAGTTCC 
AGCATTATTT ATGAGCATGA TGACTCCGAG ACCCAGGAAG ACAGTTTTGT GATCAAGTTA 
ACGGATGGTG AGCACTCTCT GGAAAAGGTG GCCCTCATCA TGATCCTCCC TGTGGATGAC 
GAGACCCCCA GGATGGCCAT CAATAATGGA CTGGAGATAG AAATTGGGGA AACCAAAGTC 
ATCAACAACA AAATATTAAT GGCCACTGAT TTGGACTCTG AAGACAAATC CTTGGCTTAT 
ATCATTCGTT ATGGGCCAGG ACATGGTGTA TTACAGAGGC GAAAACTTAC AGGTGCCTTT 
GAAAATATCA CGCTGGGCAT GAATTTTACC CAGGATGAAG TGGACAGAAA CTTAATTCAG 
TATGTCCATT TTGGGCAAGA GGGCATCCGG GACCTAATTA AATTTGATGT GACTGATGGA 
ATAAACGCCC TCATTGATCG CTACTTTTTT GTGTCCATTG GGAGTGTTGA CATTGTCTTC 
CCTGATGTGA TTAGTAAGGG GGTGTCCTTG AAGGAAGGTG GTAAAGTCAC CCTCACAACA 
GACCTACTAA GCACAAGTGA CTTGAACAGC CCCGATGAAA ACTTGGTTTT TACCATCACG 
AGGGCCCCCA TGAGAGGTCA CCTAGAGTGC ACTGACCAAC CTGGAGTGTC CATCGCATCT 
TTCACTCAGC TCCAATTGGC TGGCAACAAA ATCTACTACA TCCACACAGC TGACGATGAG 
GTGAAGATGG ACAGCTTTGA GTTTCAAGTC ACTGACGGGC GTAACCCTGT CTTCCGAACG 
TTCCGTATCT CCATTAGTGA CGTGGACAAC AAAAAGCCAG TGGTCACCAT CCATAACTTG 
GTTGTCAGCG AAGGTGAAAA CAAGCTGATC ACGCCCTTTG AACTCACTGT TGAAGACAGA 
GATACTCCCG ACAAACTCCT GAAATTCACT GTCACCCAGG TGCCTGTTCA TGGTCATCTC 
GTTTTCAATA ATACCAAACC TGCCACGGCT TTTACCAAGC AAGACTTGAA TGAAAACTTA 
ATCAGCTACA AGCATGATGG CACGGAGTCG AGTGAAGACA GCTTCTCCTT CACGGTGACC 
GATGGCACCC ATACAGACTT TTATGTTTTC CCAGATACAG TGTTTGAAAC GAGGAGACCT 
CAAGTGATGA AGATCCAGAT CTTACCTGTT GACAATAGTG TCCCCCAAAT TGCAGTGAAT 
AAAGGGGCCT CTGTGCTTCG CAATCTGGCC ACTGGTCACT TGGGGTTCAT GATCACAAGC 
AAAATATTGA AAGTGGAGGA TAGAGACAGC CTGCACTTCT CTCTGAAGTT TACTGTGACA 
GAGGCACCTC GACATGGATA TCTCTTCAAC CTGGATAAAG GCAACCACAG CATCACTCAG 
TTTACTCAAG CTGACATTGA TGACATGAAA ATATGCTATG TCTTAAAAGA AGGGGCTAAT 
GCCACAAGTG ATGTGTTCCA TTTTGCAGTT GAAGATGGTG GTGGAAACAA GTTAACCAAT 
CAGCATTTTC GTCTGAATTG GGCATGGATC TCCTTCGAAA AGGAATATAA CCTGATCAAT 
GAGGACTCCA AATTTCTCGA TGTTGTTCTT AAACGTAGAG GTTACTTGGG AGAAACTTCA 
TTTATAAGTA TTGGCACAAG AGATGGGACG GCAGAAAAGG ACAAGGACTT TAAAGGCAAA 
GCACAGAAGC AAGTGCAGTT CAACCCTGGC CAGACCAGGG CCACGTGGAG AGTGCGTATC 
CTGAGTGACG GGGAACATGA GCAATCCGAG ACCTTCCAGG TGGTTCTCTC GGAGCCTGTG 
CTGGCTGCCC TGGAGTTCCC TGCAGTGACC ACGGTAGAAA TTGTTGACCC AGGAGATGAA 
TCAACTGTGT TCATTCCCCA GTCGGAATAC TCCATTGAAG AAGATGTGGG CGAGCTGTTC 
ATTCCCATCA GGAGGACAGG AGACGTCAGC CAGGAGTTCA TGGTGATCTG TTACACACAG 
CAAGGAACAG CGACCGGAAC TGTGCCAACT TCCGTGTTGT CTTATTCGGA TTACATATCC 
AGGCCCGAGG ACCATACCAG TGTTATTCGC TTTGACAAAG ATGAAAGGGA GAAAATGTGT 
CGGATAGTTG TGATTGATGA CTCCTTATAT GAAGAAGAGG AAACGTTCCG TGTCCTTTTG 
AGCATGCCCA TGGGTGGGAG AATTGGATCA GAGTTCCCAG GAGCTCAGGT TACAATCCTC 
CCCGACAGAG ATGATGAACC CAGCTTCTAC TTTGGGAATG TGCAGTACTC TGTGGACGAG 
AGCTCCGGCC ACGTAGAGGT GCAGGTGTGG AGAACAGGTA CCGACCTCTC CAGGCCTTCC 
AGTGTCACAG TGAGATCTCG GAAGACGGAT CCTCCTTCTG CAGATGCTGG GACAGACTAT 
GTGGGAATCA GTCGCAATTT AGATTTTGCA CCTGGAGTCA ACATGCAGAC TGTTCGTGTT 
ATCATTCTGG ATGACCTTGG ACAACCAGTG CTGGAGGGAA CTGAGAAATT TGAACTGGTG 
CTTCGTATGC CTGTGAATGC TGTCCTTGGG GAGCCCAGCA AAGCCACGGT GTTCATAAAT 
GACTCTGTCT CAGACTTGCC TAAGATGCAA TTCAAAGAAC GAGTTTACAC TGGCAGCGAA 
AGTGATGGGC ATATAGCTGC TATGATCCAC AGGAGTGGTG ATATCCAGTA CAGGTCTTCA 
GTGCGATGCT ATACACGGCA GGGGTCCGCA CAGGTGATGA TGGACTTTGA AGAACGCCCA 
AACACTGACG TCTCCATCAT CACATTCCTC CCTGGTGAGA TTGAAAAGCC ATGTGTCCTT 
GAGCTGATGG ATGACATACT CTACGAGGAG GTGGAAGAGC TCCGCCTGGT ACTTGGCACT 
CCGCAAAGCA GCTCTCCCTT TGGGGCTGCC GTTGGTGAAC AGAATGAAAC TCTGATAAGG 
ATCCGAGACG ATGCTGATAA GACTATTATC AAATTTGGAG AAACCAAATT TAGCGTCACC 
GAACCCAGAG AACCAGGAGA GTCAGTCGTG GTTGAAATTC CAGTGATTCG CCAAGGAGAC 
ACTTCGAAGG TCTCCATTGT GAGAGTCCAC ACCAAGGATG GCTCTGCCAC CTCCGGAGAA 
GACTATCACC CTGTGTCAGA AGAAATTGAG TTTAAGGAAG GAGAAACCCA GCACATTGTT 
GAAATTGAAG TAATCTTCGA TGGGGTGAGA GAGATGAGAG AGGCCTTCAC TGTTCACTTA 
AAACCTGATG AAAATATGAT AGCAGAGACC CAGGTGACCA AGGCCATTGT GTATATAGAA 
GAAATGAACA GCATGGCAGA TGTCACTTTT CCTTCTGTCC CTCAAATTGT GTCCTTGCTG 
ATGTATGACG ACACGGCCAA AGCTAAGGAG AGTGCAGGAC CTGTGTCGGG CTATCCCATC 
ATCTGCGTCA CAGCTTGCAA CCCCAAATAT TCAGACTACG ATAAAACGGG CTCTATTTGT 
GCAAGTGAGA ACATCAACGA CACTCTGACC CGCTACCGAT GGCTGATTAG CGCTCCTGCT 
GGCCCTGATG GTGTGACCAG CCCCATGAGA GAGGTGGACT TCGATACCTT TTTTACATCA 
TCCAAGATGA TCACCTTAGA CTCCATATAC TTTCAGCCTG GCTCTCGGGT GCAGTGTGCG 
GCTCGTGCTG TGAACACCAA CGGGAATGAA GGTCTGGAGC TGACAAGCCC TATCGTTACC 
ATTGCCAGGG AAGAAGGTCT TTGTCAACCC CGTGTGCCTG GAGTAGTGGG GGCTGAACCG 
TTCTCAGCTA AATTGCGCTA CACAGGGCCC GAGGACCCGG ACTACTCAAA CCTTATCAAG 
CTCACAGTCA CCATGCCACA CATAGATGGC ATGCTCCCGG TGATCTCCAC CAGAGAGCTC 
TCCAACTTCG AGCTGACCCT CAGCCCCGAT GGCACCAGAG TTGGGAACCA CAAGTGCTCC 
AACCTCCTGG ACTACACTGA GGTGAAGACC CACCATGGCT TCCTGACGGA TGCCACGAAG 
AATCCAGAAG TCATTGGAGA GTCGTATCCT TACCAGTACA GCTCACCTCT CAGAGGAAAC 
GGCACCTTAC GCTTCTACCG GAACCTCAAC CTGGAGGCCT GTTTGTGGGA GTTTGTCAGC 
TACTATGACA TGTCAGAGCT CCTCGCTGAC TGCGGAGGCA CCATTGGAAC GGACGGACAG 
GTCCTAAACC TAGTGCAGTC TTACGTGACC CTTCGAGTAC CTCTGTATGT GTCCTACGTG 
TTCCACTCCC CAGTGGGAGT AGGTGGCTGG CAGCATTTTG ATTTGAAGTC GGAGCTTCGT 
CTGACTTTCG TGTATGACAC TGCCATCTTG TGGAGCAATG GAATTGGCAG CCCACCAGGA 
GCCGAACTCC AAGGATCTCT CTACCCAACG AGCATGCGCA TCAGGGAAGA GGGGCGCCTG 
GCTGTGAACT TCAAGACCCA GGCTCAGTTC CATGGCTTGT TTGTGCTGTC ACATCCCGCA 
TCTTTTACCA GCTCAATGAT CATGTCAGCT GATCATCCAG GCCTGACCTT TTCCCTCCGC 
CTTGTAAGGA GTGAACCAAC CTATAACCAG CCTATTCAGC AATGGAGCTT TGTCTCTGAC 
TTTGCAGTAC GTGACTACTC GGGAACCTAT ACTGTAAAGC TGGTGCCATG CACCACCCCG 
TCCAATCAGG AGTACCGACT GCCAGTCACC TGCAACCCCA GAGAGCCTGT CACCTTTGAC 
CTTGACATCC GATTCCAACA GGTCAGCGAT CCAGTGGCAG CTGAATTCAG CCTCAATACT 
CAGATGTACC TACTCTCCAA GAAGAGTCTC TGGTTGTCTG ACGGATCCAT GGGATTTGGA 
CAAGAAAGCG ATGTTGCTTT TGCAGAAGGT GATACAATTT ATGGTCGTGT CATGGTAGAT 
CCTGTCCAGA ATCTGGGTGA CTCCTTTTAC TGCAGCATTG AGAAGGTTTT CCTATGCACT 
GGAGCTGATG GCTATGTCCC CAAGTATAGT CCAACAAATG AAGAATATGG CTGTTTAGCC 
GACTCTCCTT CACTTCTGTA TAGATTTAAA ATTGTGGACA AAGCTCAACC AGAGACACAA 
GCCACCAGTT TTGGAAATGT CCTCTTTAAT GCCAAACTGG CAGTGGATGA CCCTGAAGCC 
ATCCTCTTAG TGAATCAGCC TGGCTCTGAT GGATTTAAAG TCGACTCAAC ACCACTCTTT 
CAGGTTGCTC TGGGCCGAGA ATGGTACATA CACACAATCT ATACAGTAAG ATCAAAAGAC 
AACACCAATC GAGGTATCGG CAAAAGAAGT GTGGAGTATG AGTACCACTC TCTGGTGCAT 
CAAGGGAAGC CACAGATAAC TACCGGGAGC CGGAAGAAGA GAGAGATCAG GAGCGCACCC 
CCACTGGCAT GGGACATCGG TGCTGAAAAC AACCGAGGGA CAAACATCCA GCACATTGCC 
CTGGACCGCG CCAACAAGAG GCAGACCCCC CCAGGGAGAG TGCCCCCCGA AGGCATCCTA 
CCCCGGGAGC TCAACAGTCC CAGCTCTGAG GTCAGTCTGG TCACCATCGT GGGCAGCATC 
GCGGTGGGAT TACTCACCAT CTGCCTCACT GTCATTGCAG CAATGATGTG CAAAAGCAAG 
AAAAGTGTCA GGGGTAAGGA CACTCCGAAG GGCTCTGGCA GCAGCGAGCC CATGATGCCC 
CCGCAGAGCC ACCATAGCGA CAGCTCAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Frem2 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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