Trio cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trio
Entrez Gene ID	101959859
Full Name	trio Rho guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005336753.2	XP_005336810.1	triple functional domain protein
XM_021734206.1	XP_021589881.1	triple functional domain protein isoform X2
XM_005336753.3	XP_005336810.1	triple functional domain protein isoform X1

Pathways from BioSystems
Gene Ontology

The following Trio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc67546	XM_005336753.3 Latest version!	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83524	XM_021734206.1 Latest version!	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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OIc67546	XM_005336753.2	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trio ( XM_005336753.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005336810.1 Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), mRNA.

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CloneID	OIc67546
Clone ID Related Accession (Same CDS sequence)	XM_005336753.2 , XM_005336753.3
Accession Version	XM_005336753.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	triple functional domain protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936665.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005336753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTCTG AAAGAAAAAG TTGCTTATCT TTCAGGTGGT 
AGAGATAAGC GTGGAGGTCC CATTTTAACA TTTCCAGCCC GCAGCAATCA TGACAGAATA 
CGGCAGGAGG ATCTCAGGAG ACTCATTTCA TATCTAGCCT GCATTCCTAG TGAGGAAGTC 
TGCAAGCGAG GCTTCACGGT GATTGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCCCTACTGA AGATCCTGCA GGAATCCTTC CCCTGCTGCA TCCACGTCGC CCTGATAATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACGAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAACA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTCG ATGGCTGCCT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC 
ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC CTAGAAGGGG CTCGGAATAT GATAGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAA GCCCCCATTG AGGACTTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC GATAGTTTTC CCAAAAAGAA CTCAGGGTCA 
GGTAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTAGA TAGGCTGCAC 
TCGACCCGGC AGCATCTGCA TCAGATGTGG CATGTGAGGA AGTTGAAGCT GGACCAATGC 
TTTCAGCTGA GGCTGTTTGA ACAAGATGCT GAAAAGATGT TTGACTGGAT TACACACAAC 
AAAGGCCTGT TTCTAAACAG TTACACAGAG ATTGGGACCA GCCACCCTCA TGCAATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCTATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATAATGT CGGTGGCCAA TCGCTTGGTG GAATCTGGCC ACTATGCCTC TCAGCAGATC 
AAGCAGATTG CAAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGTAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTACATGAGC 
AACGTGGACT CCTGGTGTAA AGCCTGTGGT GAAGTAGACC TTCCATCTGA GCTGCAGGAT 
CTGGAAGATG CCATCCATCA CCATCAGGGA ATCTACGAGC ACATCACCCT TGCTTATTCT 
GAGGTGAGCC AAGATGGAAA GTCACTTCTT GACAAGCTCC AGAGGCCCTT GACTCCTGGC 
AGCTCTGACT CCCTAACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTGGAT 
GTCATCCATG AGGTGCTGCA CCATCAACGG CAGCTTGAGA ACATCTGGCA ACACCGCAAA 
GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA ACAGGTGCTG 
GATTGGATCG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATACACCA ATGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAG 
TGCGACCCAG AAGAGATTTA TCAGGCTGCC CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TTGAGCAACG AAAGATTCTC CTGGACATGT CGGTGTCCTT TCACACCCAC 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG TTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACGCTGCAGG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATTCA GCAGCTGAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CATAACAGCT CCATCAACCA CATCGAGACG 
GTGCTGCAGC AGCTGGACGA GGCCCAGTCA CAGATGGAGG AACTCTTCCA GGAGCGCAAG 
ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC ATATTTGAGA GGGATGCCAT AGATATCATC 
TCAGACCTTG AGTCTTGGAA TGATGAGCTT TCTCAACAAA TGAATGACTT TGACACAGAA 
GATCTCACAA TTGCAGAACA GCGCCTCCAG CACCATGCAG ACAAGGCCTT GACCATGAAT 
AACTTGACTT TTGATGTCAT TCACCAAGGG CAGGATCTTC TGCAGTATGT CAATGAAGTC 
CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT AGAGATGTAG ATATGGCAAC TCGAGTCCAG 
GACCTGTTGG AGTTTCTTCA TGAAAAGCAG CAGGAGTTGG ATTTAGCTGC AGAACAGCAC 
CGGAAGCACC TGGAGCAGTG CGTGCAGCTG CGCCATCTAC AGGCAGAGGT GAAACAGGTG 
TTGGGGTGGA TACGCAATGG AGAGTCTATG TTAAATGCCG GGCTGATCAC AGCCAGTTCA 
CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG CATGAGCAGT TCCAGCATGC TATCGAGAAA 
ACGCACCAGA GTGCACTGCA GGTGCAGCAG AAGGCTGAGG CAATGCTTCA GGCCAATCAC 
TATGACATGG ACATGATCCG GGACTGTGCT GAGAAGGTGG CCTCACACTG GCAGCAGCTC 
ATGCTCAAAA TGGAAGATCG CCTCAAGCTT GTCAATGCCT CTGTTGCCTT CTACAAAACT 
TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC CTAGAGCAGG AATACAAGAG AGAGGAAGAC 
TGGTGTGGTG GAGCTGACAA GCTGGGCCCC AATTCTGAGA CAGACCATGT CACCCCGATG 
ATCAGCAAGC ACCTTGAACA AAAGGAGGCT TTCCTAAAGG CTTGCACACT TGCTAGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTACAC AGGAACAGTG TGAACATGCC AGGAATGGTG 
ACACACATCA AGGCTCCAGA GCAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGGAAGAGAC GGCTGGACCA GTGCCAGCAG 
TATGTGGTCT TTGAGCGAAG TGCCAAGCAG GCTTTAGAAT GGATTCATGA CAACGGGGAG 
TTCTACCTTT CCACACATAC CTCAACAGGG TCCAGCATAC AACACACCCA GGAGCTTCTG 
AAAGAACATG AGGAGTTTCA GATAACTGCG AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA AATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAATACAGG 
ACCTCTTTGG AGAAAGCTCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTT 
CAGCTTGATA TCATCCCAGC CAGTATCCCT GGGTCAGAGG TGAAGCTTCG AGATGCTGCT 
CATGAACTCA ATGAAGAAAA ACGGAAGTCT GCCCGCAGGA AAGAGTTCAT AATGGCAGAG 
CTAATTCAAA CTGAAAAGGC TTATGTAAGA GACCTTCGGG AGTGTATGGA TACCTACCTA 
TGGGAAATGA CTAGTGGAGT GGAGGAGATC CCGCCTGGCA TTGTAAACAA AGAGCTCATT 
ATCTTTGGAA ACATGCAGGA AATCTATGAA TTTCATAATA ACATCTTCCT AAAGGAGCTG 
GAAAAATATG AACAACTGCC AGAGGATGTT GGACATTGCT TTGTTACCTG GGCAGACAAG 
TTTCAGATGT ATGTCACCTA TTGCAAAAAT AAGCCTGATT CTACTCAGCT TATATTGGAA 
CATGCAGGGT CCTACTTTGA TGAGATACAG CAGCGGCATG GATTAGCCAA TTCCATCTCT 
TCTTACCTTA TTAAACCAGT TCAGCGAATA ACAAAATATC AGCTCCTTTT GAAAGAGCTG 
CTCACGTGCT GTGAGGAAGG AAAGGGAGAA ATTAAAGACG GCCTTGAGGT GATGCTTAGT 
GTGCCAAAGC GAGCCAACGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAGCT CATCTTGCAA GAGTCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AAGGTCGAGA GCGGCATCTT TTTCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTAA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ACAAAAGCAA ATTATTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGGGATCCTT GCAAATTTGC ACTGTGGGTA 
GGGAGGACGC CAACTTCAGA TAACAAAATT GTCCTTAAGG CTTCCAGTAT CGAGAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAAGTA ATACAGGAAA GGACCATCCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATCCCCAAA ACAGCGCCAG CCACGAGACA GAAGGGAAGG 
AGGGATGGAG AGGACCTGGA CAGCCAAGGG GATGGCAGCA GCCAGCCTGA TACAATTTCC 
ATCGCCTCTC GGACGTCTCA GAACACATTG GATAGCGATA AGCTCTCAGG TGGCTGTGAG 
CTGACGGTTG TGATTCACGA CTTCACGGCC TGCAACAGTA ACGAGCTCAC CATCCGGCGC 
GGGCAAACAG TGGAGGTTTT GGAGCGGCCA CACGACAAGC CTGACTGGTG CCTGGTGCGC 
ACCACGGACC GGTCCCCGGC AGCGGAAGGC CTGGTGCCCT GCGGCTCTCT GTGCATTGCC 
CACTCCAGAA GTAGCATGGA GATGGAGGGC ATCTTCAACC ACAAAGACTC CCTCTCTGTC 
TCCAGTAACG ATGCCAGTCC ACCTGCCTCC GTAGCTTCCC TTCAGCCCCA CATGATCGGG 
GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA GGCAACACCT TGCGCAAGTG GCTTACCAGC 
CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT GATGGGCACG TGAAGAAGCT TGCCCACAAG 
CACAAAAAGA GCAGGGAGGT CCGCAAGAGC ACAGACGCTG GCTCGCAGAA GGACTCGGAT 
GACAGTGCAG CCACTCCTCA GGACGAGACT GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC 
AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG TCAGGCATGC AGAGCTGTGG AGAAGAAGAA 
GGGGAGGAGG GGGCCGATGC TGTGCCACTC CCCCCACCCA TGGCTATCCA GCAGCACAGC 
CTCCTTCAGC CGGACTCGCA GGATGACAAG GCCTCTTCTC GATTACTAGT CCGCCCCACC 
AGCTCCGAAA CTCCAAGCGC AGCCGAACTT GTCAGTGCAA TCGAGGAACT CGTGAAGAGC 
AAGATGGCAC TGGAGGATCG TCCTAGCTCC CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCATCGGA CAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGGCACTACG TCTTGCAAGA ATTAGTGGAG 
ACAGAGCGTG ACTATGTACG AGACCTTGGC TACGTGGTTG AAGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAG GGAAAAGACA AGATTGTGTT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA 
GATCCAGAAA AACTAGGATC ACTCTTTGTG AAACACGAGA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAACG CCTTGGCCAC AGACTGCAGC TCACAGATTT GTTAATCAAA 
CCTGTGCAGA GAATTATGAA GTACCAGCTG TTGCTGAAGG ACTTCCTTAA GTACTCCAAA 
AAAGCTAGTC TGGACACATC AGAATTAGAG AAAGCCGTGG AAGTCATGTG CATAGTCCCA 
AAGCGGTGCA ACGACATGAT GAACGTGGGG AGGCTGCAAG GATTCGACGG TAAAATCGTC 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTTTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC CTCTTTGAAC AGATTGTCAT ATTCAGTGAA 
CCACTAGACA AAAAAAAGGG TTTCTCCATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTCTGACA 
TCAAGGACAG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCAAATCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CCATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCAG TGGCAGCGGG 
GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC 
CATGGCAGTG GTCCCAGCAG CTGCAGCAGT GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC 
CGGATCCCAC AGCCTGTCAG ACACCACCCC CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTATG TCTGCTCCCA GCCCCTCACT GCCTCCTCCC 
AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC GGCCAACCCA GCAGGCTCCC CTTGAGCGGA 
GACTCAGAAG GTCCTGAGCG AGAAACGGAA CCCATCCCCA AGATGAAGGT GATGGACAGC 
CCCAGGAAAA CCTCCGGGAA CACTTCCGGT GCAAATCAAG ATGGAAATGC CAAGGACGCT 
CGGGGGAACC TGGGCCCTCT GCCCCTGAGC AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG 
AACTCTCCTC TTTCTACCAC AGTCCCTTCT CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC 
AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG AGCTCCATCC CCTCCTCCCC GGCAAGCCGG 
CCGGGCTCCT TCACTTTCCC GGGGGACAGC GACTCCCTCC AGCGGCAGAC ACATCGCCAC 
ACAGCCCCCA GCAAGGACAC GGACCGCATG AGCACGTGCT CCTCAGCCAG CGAGCAGTCC 
GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGTAGCAGCA GTAGCAACAT CTCCACCATG 
TTGGTGACAC ACGATTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG 
GTCGTTCAAA TTCTGGCCAG CAACCAGCAG AACATGTTTC TGGTGTTCCG TGCCGCCACT 
GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGCCA CACCAGTGCA 
GTCATCATGG AGAACCCCGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC 
CGTTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AAAGGGAGGG CAAGTTAGAG 
AATGGTTATC GGAAATCCCG GGAAGGGCTC AGCAACAAGG TATCTGTGAA GCTTCTCAAT 
CCCAACTACA TTTATGATGT TCCCCCAGAA TTCGTCATCC CATTGAGTGA GGTCACGTGT 
GAGACAGGGG AGACCGTTGT TCTTAGATGT CGTGTCTGTG GCCGCCCCAA GGCCTCAATC 
ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GCCACTTCAG CATCTCCTAC 
AGTGACTTGG GGGAGGCCGC TCTGAAGATC ATTGGTGTGA CCACGGAAGA TGATGGCATC 
TACACATGTA TTGCTGTGAA CGACATGGGT TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT 
CTAGGTCCAG GGAGTGATGG GATCATGGTG ACCTGGAAAG ACAACTTTGA CTCCTTCTAC 
AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA TTTGCTGTCG TTAAGAAATG TGATCAGAAA 
GGAACCAAAC GAGCAGTGGC CACAAAGTTT GTGAACAAGA AGTTGATGAA GCGTGACCAG 
GTCACCCATG AGCTTGGCAT CCTGCAGAAC CTCCAGCACC CGCTCCTCAT TGGCCTTCTC 
GACACCTTCG AGACCCCCAC CAGCTACGTC CTGGTCTTGG AGATGGCTGA CCAGGGTCGC 
CTCCTGGACT GCGTGGTACG ATGGGGAAGC CTCACTGAAG GAAAGATCAG AGCACACCTG 
GGGGAGGTTC TGGAAGCCGT CCGATACCTT CACAACTGCA GGATAGCACA CCTGGACCTA 
AAGCCTGAGA ATATTCTGGT GGATCAGAGT TTAGCCAAGC CAACAATTAA ACTGGCTGAC 
TTTGGAGATG CTGTCCAGCT CAACACGACC TACTACATCC ACCAGTTACT GGGGAACCCT 
GAGTTTGCAG CTCCTGAAAT CATTCTTGGG AACCCTGTCT CCCTGACCTC GGACACATGG 
AGTGTTGGAG TGCTCACGTA CGTGCTTCTT AGCGGTGTGT CCCCCTTCCT GGATGACAGC 
GTGGAAGAGA CCTGCCTGAA TATTTGCCGA TTAGACTTTA GTTTCCCAGA TGACTACTTT 
AAAGGAGTTA GCCAGAAGGC CAAGGAATTC GTGTGCTTCC TCCTGCAGGA GGACCCTGCC 
AAGCGTCCCT CGGCTGCACT GGCTCTCCAG GAGCAGTGGC TACAGGTGGG CAACGGCAAC 
GGCAAAGGCT CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA 
CACCAGAATG ATGTTCGACC TATTCGTAGC ATTAAAAACT TTCTACAGAG CAGGCTTCTG 
CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005336810.1
CDS	19..9150
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQELIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETAEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSNPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSSSGGGGSSGGASGGGGSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGMSAPSPSLPPPSSSLAFEASLGQPSRLPLSGDSEGPERETEPIPKMKVMDSPRKTSGNTSGANQDGNAKDARGNLGPLPLSKPRPGAISPLNSPLSTTVPSPFGKEPFPPSSPLQKGGSFWSSIPSSPASRPGSFTFPGDSDSLQRQTHRHTAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHFSISYSDLGEAALKIIGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFAVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQVGNGNGKGSGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_005336753.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005336753.2

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTCTG AAAGAAAAAG TTGCTTATCT TTCAGGTGGT 
AGAGATAAGC GTGGAGGTCC CATTTTAACA TTTCCAGCCC GCAGCAATCA TGACAGAATA 
CGGCAGGAGG ATCTCAGGAG ACTCATTTCA TATCTAGCCT GCATTCCTAG TGAGGAAGTC 
TGCAAGCGAG GCTTCACGGT GATTGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCCCTACTGA AGATCCTGCA GGAATCCTTC CCCTGCTGCA TCCACGTCGC CCTGATAATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACGAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAACA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTCG ATGGCTGCCT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC 
ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC CTAGAAGGGG CTCGGAATAT GATAGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAA GCCCCCATTG AGGACTTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC GATAGTTTTC CCAAAAAGAA CTCAGGGTCA 
GGTAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTAGA TAGGCTGCAC 
TCGACCCGGC AGCATCTGCA TCAGATGTGG CATGTGAGGA AGTTGAAGCT GGACCAATGC 
TTTCAGCTGA GGCTGTTTGA ACAAGATGCT GAAAAGATGT TTGACTGGAT TACACACAAC 
AAAGGCCTGT TTCTAAACAG TTACACAGAG ATTGGGACCA GCCACCCTCA TGCAATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCTATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATAATGT CGGTGGCCAA TCGCTTGGTG GAATCTGGCC ACTATGCCTC TCAGCAGATC 
AAGCAGATTG CAAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGTAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTACATGAGC 
AACGTGGACT CCTGGTGTAA AGCCTGTGGT GAAGTAGACC TTCCATCTGA GCTGCAGGAT 
CTGGAAGATG CCATCCATCA CCATCAGGGA ATCTACGAGC ACATCACCCT TGCTTATTCT 
GAGGTGAGCC AAGATGGAAA GTCACTTCTT GACAAGCTCC AGAGGCCCTT GACTCCTGGC 
AGCTCTGACT CCCTAACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTGGAT 
GTCATCCATG AGGTGCTGCA CCATCAACGG CAGCTTGAGA ACATCTGGCA ACACCGCAAA 
GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA ACAGGTGCTG 
GATTGGATCG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATACACCA ATGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAG 
TGCGACCCAG AAGAGATTTA TCAGGCTGCC CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TTGAGCAACG AAAGATTCTC CTGGACATGT CGGTGTCCTT TCACACCCAC 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG TTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACGCTGCAGG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATTCA GCAGCTGAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CATAACAGCT CCATCAACCA CATCGAGACG 
GTGCTGCAGC AGCTGGACGA GGCCCAGTCA CAGATGGAGG AACTCTTCCA GGAGCGCAAG 
ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC ATATTTGAGA GGGATGCCAT AGATATCATC 
TCAGACCTTG AGTCTTGGAA TGATGAGCTT TCTCAACAAA TGAATGACTT TGACACAGAA 
GATCTCACAA TTGCAGAACA GCGCCTCCAG CACCATGCAG ACAAGGCCTT GACCATGAAT 
AACTTGACTT TTGATGTCAT TCACCAAGGG CAGGATCTTC TGCAGTATGT CAATGAAGTC 
CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT AGAGATGTAG ATATGGCAAC TCGAGTCCAG 
GACCTGTTGG AGTTTCTTCA TGAAAAGCAG CAGGAGTTGG ATTTAGCTGC AGAACAGCAC 
CGGAAGCACC TGGAGCAGTG CGTGCAGCTG CGCCATCTAC AGGCAGAGGT GAAACAGGTG 
TTGGGGTGGA TACGCAATGG AGAGTCTATG TTAAATGCCG GGCTGATCAC AGCCAGTTCA 
CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG CATGAGCAGT TCCAGCATGC TATCGAGAAA 
ACGCACCAGA GTGCACTGCA GGTGCAGCAG AAGGCTGAGG CAATGCTTCA GGCCAATCAC 
TATGACATGG ACATGATCCG GGACTGTGCT GAGAAGGTGG CCTCACACTG GCAGCAGCTC 
ATGCTCAAAA TGGAAGATCG CCTCAAGCTT GTCAATGCCT CTGTTGCCTT CTACAAAACT 
TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC CTAGAGCAGG AATACAAGAG AGAGGAAGAC 
TGGTGTGGTG GAGCTGACAA GCTGGGCCCC AATTCTGAGA CAGACCATGT CACCCCGATG 
ATCAGCAAGC ACCTTGAACA AAAGGAGGCT TTCCTAAAGG CTTGCACACT TGCTAGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTACAC AGGAACAGTG TGAACATGCC AGGAATGGTG 
ACACACATCA AGGCTCCAGA GCAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGGAAGAGAC GGCTGGACCA GTGCCAGCAG 
TATGTGGTCT TTGAGCGAAG TGCCAAGCAG GCTTTAGAAT GGATTCATGA CAACGGGGAG 
TTCTACCTTT CCACACATAC CTCAACAGGG TCCAGCATAC AACACACCCA GGAGCTTCTG 
AAAGAACATG AGGAGTTTCA GATAACTGCG AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA AATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAATACAGG 
ACCTCTTTGG AGAAAGCTCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTT 
CAGCTTGATA TCATCCCAGC CAGTATCCCT GGGTCAGAGG TGAAGCTTCG AGATGCTGCT 
CATGAACTCA ATGAAGAAAA ACGGAAGTCT GCCCGCAGGA AAGAGTTCAT AATGGCAGAG 
CTAATTCAAA CTGAAAAGGC TTATGTAAGA GACCTTCGGG AGTGTATGGA TACCTACCTA 
TGGGAAATGA CTAGTGGAGT GGAGGAGATC CCGCCTGGCA TTGTAAACAA AGAGCTCATT 
ATCTTTGGAA ACATGCAGGA AATCTATGAA TTTCATAATA ACATCTTCCT AAAGGAGCTG 
GAAAAATATG AACAACTGCC AGAGGATGTT GGACATTGCT TTGTTACCTG GGCAGACAAG 
TTTCAGATGT ATGTCACCTA TTGCAAAAAT AAGCCTGATT CTACTCAGCT TATATTGGAA 
CATGCAGGGT CCTACTTTGA TGAGATACAG CAGCGGCATG GATTAGCCAA TTCCATCTCT 
TCTTACCTTA TTAAACCAGT TCAGCGAATA ACAAAATATC AGCTCCTTTT GAAAGAGCTG 
CTCACGTGCT GTGAGGAAGG AAAGGGAGAA ATTAAAGACG GCCTTGAGGT GATGCTTAGT 
GTGCCAAAGC GAGCCAACGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAGCT CATCTTGCAA GAGTCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AAGGTCGAGA GCGGCATCTT TTTCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTAA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ACAAAAGCAA ATTATTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGGGATCCTT GCAAATTTGC ACTGTGGGTA 
GGGAGGACGC CAACTTCAGA TAACAAAATT GTCCTTAAGG CTTCCAGTAT CGAGAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAAGTA ATACAGGAAA GGACCATCCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATCCCCAAA ACAGCGCCAG CCACGAGACA GAAGGGAAGG 
AGGGATGGAG AGGACCTGGA CAGCCAAGGG GATGGCAGCA GCCAGCCTGA TACAATTTCC 
ATCGCCTCTC GGACGTCTCA GAACACATTG GATAGCGATA AGCTCTCAGG TGGCTGTGAG 
CTGACGGTTG TGATTCACGA CTTCACGGCC TGCAACAGTA ACGAGCTCAC CATCCGGCGC 
GGGCAAACAG TGGAGGTTTT GGAGCGGCCA CACGACAAGC CTGACTGGTG CCTGGTGCGC 
ACCACGGACC GGTCCCCGGC AGCGGAAGGC CTGGTGCCCT GCGGCTCTCT GTGCATTGCC 
CACTCCAGAA GTAGCATGGA GATGGAGGGC ATCTTCAACC ACAAAGACTC CCTCTCTGTC 
TCCAGTAACG ATGCCAGTCC ACCTGCCTCC GTAGCTTCCC TTCAGCCCCA CATGATCGGG 
GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA GGCAACACCT TGCGCAAGTG GCTTACCAGC 
CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT GATGGGCACG TGAAGAAGCT TGCCCACAAG 
CACAAAAAGA GCAGGGAGGT CCGCAAGAGC ACAGACGCTG GCTCGCAGAA GGACTCGGAT 
GACAGTGCAG CCACTCCTCA GGACGAGACT GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC 
AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG TCAGGCATGC AGAGCTGTGG AGAAGAAGAA 
GGGGAGGAGG GGGCCGATGC TGTGCCACTC CCCCCACCCA TGGCTATCCA GCAGCACAGC 
CTCCTTCAGC CGGACTCGCA GGATGACAAG GCCTCTTCTC GATTACTAGT CCGCCCCACC 
AGCTCCGAAA CTCCAAGCGC AGCCGAACTT GTCAGTGCAA TCGAGGAACT CGTGAAGAGC 
AAGATGGCAC TGGAGGATCG TCCTAGCTCC CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCATCGGA CAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGGCACTACG TCTTGCAAGA ATTAGTGGAG 
ACAGAGCGTG ACTATGTACG AGACCTTGGC TACGTGGTTG AAGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAG GGAAAAGACA AGATTGTGTT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA 
GATCCAGAAA AACTAGGATC ACTCTTTGTG AAACACGAGA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAACG CCTTGGCCAC AGACTGCAGC TCACAGATTT GTTAATCAAA 
CCTGTGCAGA GAATTATGAA GTACCAGCTG TTGCTGAAGG ACTTCCTTAA GTACTCCAAA 
AAAGCTAGTC TGGACACATC AGAATTAGAG AAAGCCGTGG AAGTCATGTG CATAGTCCCA 
AAGCGGTGCA ACGACATGAT GAACGTGGGG AGGCTGCAAG GATTCGACGG TAAAATCGTC 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTTTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC CTCTTTGAAC AGATTGTCAT ATTCAGTGAA 
CCACTAGACA AAAAAAAGGG TTTCTCCATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTCTGACA 
TCAAGGACAG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCAAATCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CCATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCAG TGGCAGCGGG 
GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC 
CATGGCAGTG GTCCCAGCAG CTGCAGCAGT GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC 
CGGATCCCAC AGCCTGTCAG ACACCACCCC CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTATG TCTGCTCCCA GCCCCTCACT GCCTCCTCCC 
AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC GGCCAACCCA GCAGGCTCCC CTTGAGCGGA 
GACTCAGAAG GTCCTGAGCG AGAAACGGAA CCCATCCCCA AGATGAAGGT GATGGACAGC 
CCCAGGAAAA CCTCCGGGAA CACTTCCGGT GCAAATCAAG ATGGAAATGC CAAGGACGCT 
CGGGGGAACC TGGGCCCTCT GCCCCTGAGC AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG 
AACTCTCCTC TTTCTACCAC AGTCCCTTCT CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC 
AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG AGCTCCATCC CCTCCTCCCC GGCAAGCCGG 
CCGGGCTCCT TCACTTTCCC GGGGGACAGC GACTCCCTCC AGCGGCAGAC ACATCGCCAC 
ACAGCCCCCA GCAAGGACAC GGACCGCATG AGCACGTGCT CCTCAGCCAG CGAGCAGTCC 
GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGTAGCAGCA GTAGCAACAT CTCCACCATG 
TTGGTGACAC ACGATTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG 
GTCGTTCAAA TTCTGGCCAG CAACCAGCAG AACATGTTTC TGGTGTTCCG TGCCGCCACT 
GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGCCA CACCAGTGCA 
GTCATCATGG AGAACCCCGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC 
CGTTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AAAGGGAGGG CAAGTTAGAG 
AATGGTTATC GGAAATCCCG GGAAGGGCTC AGCAACAAGG TATCTGTGAA GCTTCTCAAT 
CCCAACTACA TTTATGATGT TCCCCCAGAA TTCGTCATCC CATTGAGTGA GGTCACGTGT 
GAGACAGGGG AGACCGTTGT TCTTAGATGT CGTGTCTGTG GCCGCCCCAA GGCCTCAATC 
ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GCCACTTCAG CATCTCCTAC 
AGTGACTTGG GGGAGGCCGC TCTGAAGATC ATTGGTGTGA CCACGGAAGA TGATGGCATC 
TACACATGTA TTGCTGTGAA CGACATGGGT TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT 
CTAGGTCCAG GGAGTGATGG GATCATGGTG ACCTGGAAAG ACAACTTTGA CTCCTTCTAC 
AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA TTTGCTGTCG TTAAGAAATG TGATCAGAAA 
GGAACCAAAC GAGCAGTGGC CACAAAGTTT GTGAACAAGA AGTTGATGAA GCGTGACCAG 
GTCACCCATG AGCTTGGCAT CCTGCAGAAC CTCCAGCACC CGCTCCTCAT TGGCCTTCTC 
GACACCTTCG AGACCCCCAC CAGCTACGTC CTGGTCTTGG AGATGGCTGA CCAGGGTCGC 
CTCCTGGACT GCGTGGTACG ATGGGGAAGC CTCACTGAAG GAAAGATCAG AGCACACCTG 
GGGGAGGTTC TGGAAGCCGT CCGATACCTT CACAACTGCA GGATAGCACA CCTGGACCTA 
AAGCCTGAGA ATATTCTGGT GGATCAGAGT TTAGCCAAGC CAACAATTAA ACTGGCTGAC 
TTTGGAGATG CTGTCCAGCT CAACACGACC TACTACATCC ACCAGTTACT GGGGAACCCT 
GAGTTTGCAG CTCCTGAAAT CATTCTTGGG AACCCTGTCT CCCTGACCTC GGACACATGG 
AGTGTTGGAG TGCTCACGTA CGTGCTTCTT AGCGGTGTGT CCCCCTTCCT GGATGACAGC 
GTGGAAGAGA CCTGCCTGAA TATTTGCCGA TTAGACTTTA GTTTCCCAGA TGACTACTTT 
AAAGGAGTTA GCCAGAAGGC CAAGGAATTC GTGTGCTTCC TCCTGCAGGA GGACCCTGCC 
AAGCGTCCCT CGGCTGCACT GGCTCTCCAG GAGCAGTGGC TACAGGTGGG CAACGGCAAC 
GGCAAAGGCT CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA 
CACCAGAATG ATGTTCGACC TATTCGTAGC ATTAAAAACT TTCTACAGAG CAGGCTTCTG 
CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trio ( XM_005336753.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005336810.1 Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X1, mRNA.

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CloneID	OIc67546
Clone ID Related Accession (Same CDS sequence)	XM_005336753.2 , XM_005336753.3
Accession Version	XM_005336753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	triple functional domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936665.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005336753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT GCCAATTCTG AAAGAAAAAG TTGCTTATCT TTCAGGTGGT 
AGAGATAAGC GTGGAGGTCC CATTTTAACA TTTCCAGCCC GCAGCAATCA TGACAGAATA 
CGGCAGGAGG ATCTCAGGAG ACTCATTTCA TATCTAGCCT GCATTCCTAG TGAGGAAGTC 
TGCAAGCGAG GCTTCACGGT GATTGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCCCTACTGA AGATCCTGCA GGAATCCTTC CCCTGCTGCA TCCACGTCGC CCTGATAATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACGAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAACA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTCG ATGGCTGCCT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC 
ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC CTAGAAGGGG CTCGGAATAT GATAGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAA GCCCCCATTG AGGACTTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC GATAGTTTTC CCAAAAAGAA CTCAGGGTCA 
GGTAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTAGA TAGGCTGCAC 
TCGACCCGGC AGCATCTGCA TCAGATGTGG CATGTGAGGA AGTTGAAGCT GGACCAATGC 
TTTCAGCTGA GGCTGTTTGA ACAAGATGCT GAAAAGATGT TTGACTGGAT TACACACAAC 
AAAGGCCTGT TTCTAAACAG TTACACAGAG ATTGGGACCA GCCACCCTCA TGCAATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCTATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATAATGT CGGTGGCCAA TCGCTTGGTG GAATCTGGCC ACTATGCCTC TCAGCAGATC 
AAGCAGATTG CAAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGTAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTACATGAGC 
AACGTGGACT CCTGGTGTAA AGCCTGTGGT GAAGTAGACC TTCCATCTGA GCTGCAGGAT 
CTGGAAGATG CCATCCATCA CCATCAGGGA ATCTACGAGC ACATCACCCT TGCTTATTCT 
GAGGTGAGCC AAGATGGAAA GTCACTTCTT GACAAGCTCC AGAGGCCCTT GACTCCTGGC 
AGCTCTGACT CCCTAACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTGGAT 
GTCATCCATG AGGTGCTGCA CCATCAACGG CAGCTTGAGA ACATCTGGCA ACACCGCAAA 
GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA ACAGGTGCTG 
GATTGGATCG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATACACCA ATGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAG 
TGCGACCCAG AAGAGATTTA TCAGGCTGCC CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TTGAGCAACG AAAGATTCTC CTGGACATGT CGGTGTCCTT TCACACCCAC 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG TTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACGCTGCAGG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATTCA GCAGCTGAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CATAACAGCT CCATCAACCA CATCGAGACG 
GTGCTGCAGC AGCTGGACGA GGCCCAGTCA CAGATGGAGG AACTCTTCCA GGAGCGCAAG 
ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC ATATTTGAGA GGGATGCCAT AGATATCATC 
TCAGACCTTG AGTCTTGGAA TGATGAGCTT TCTCAACAAA TGAATGACTT TGACACAGAA 
GATCTCACAA TTGCAGAACA GCGCCTCCAG CACCATGCAG ACAAGGCCTT GACCATGAAT 
AACTTGACTT TTGATGTCAT TCACCAAGGG CAGGATCTTC TGCAGTATGT CAATGAAGTC 
CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT AGAGATGTAG ATATGGCAAC TCGAGTCCAG 
GACCTGTTGG AGTTTCTTCA TGAAAAGCAG CAGGAGTTGG ATTTAGCTGC AGAACAGCAC 
CGGAAGCACC TGGAGCAGTG CGTGCAGCTG CGCCATCTAC AGGCAGAGGT GAAACAGGTG 
TTGGGGTGGA TACGCAATGG AGAGTCTATG TTAAATGCCG GGCTGATCAC AGCCAGTTCA 
CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG CATGAGCAGT TCCAGCATGC TATCGAGAAA 
ACGCACCAGA GTGCACTGCA GGTGCAGCAG AAGGCTGAGG CAATGCTTCA GGCCAATCAC 
TATGACATGG ACATGATCCG GGACTGTGCT GAGAAGGTGG CCTCACACTG GCAGCAGCTC 
ATGCTCAAAA TGGAAGATCG CCTCAAGCTT GTCAATGCCT CTGTTGCCTT CTACAAAACT 
TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC CTAGAGCAGG AATACAAGAG AGAGGAAGAC 
TGGTGTGGTG GAGCTGACAA GCTGGGCCCC AATTCTGAGA CAGACCATGT CACCCCGATG 
ATCAGCAAGC ACCTTGAACA AAAGGAGGCT TTCCTAAAGG CTTGCACACT TGCTAGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTACAC AGGAACAGTG TGAACATGCC AGGAATGGTG 
ACACACATCA AGGCTCCAGA GCAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGGAAGAGAC GGCTGGACCA GTGCCAGCAG 
TATGTGGTCT TTGAGCGAAG TGCCAAGCAG GCTTTAGAAT GGATTCATGA CAACGGGGAG 
TTCTACCTTT CCACACATAC CTCAACAGGG TCCAGCATAC AACACACCCA GGAGCTTCTG 
AAAGAACATG AGGAGTTTCA GATAACTGCG AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA AATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAATACAGG 
ACCTCTTTGG AGAAAGCTCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTT 
CAGCTTGATA TCATCCCAGC CAGTATCCCT GGGTCAGAGG TGAAGCTTCG AGATGCTGCT 
CATGAACTCA ATGAAGAAAA ACGGAAGTCT GCCCGCAGGA AAGAGTTCAT AATGGCAGAG 
CTAATTCAAA CTGAAAAGGC TTATGTAAGA GACCTTCGGG AGTGTATGGA TACCTACCTA 
TGGGAAATGA CTAGTGGAGT GGAGGAGATC CCGCCTGGCA TTGTAAACAA AGAGCTCATT 
ATCTTTGGAA ACATGCAGGA AATCTATGAA TTTCATAATA ACATCTTCCT AAAGGAGCTG 
GAAAAATATG AACAACTGCC AGAGGATGTT GGACATTGCT TTGTTACCTG GGCAGACAAG 
TTTCAGATGT ATGTCACCTA TTGCAAAAAT AAGCCTGATT CTACTCAGCT TATATTGGAA 
CATGCAGGGT CCTACTTTGA TGAGATACAG CAGCGGCATG GATTAGCCAA TTCCATCTCT 
TCTTACCTTA TTAAACCAGT TCAGCGAATA ACAAAATATC AGCTCCTTTT GAAAGAGCTG 
CTCACGTGCT GTGAGGAAGG AAAGGGAGAA ATTAAAGACG GCCTTGAGGT GATGCTTAGT 
GTGCCAAAGC GAGCCAACGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAGCT CATCTTGCAA GAGTCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AAGGTCGAGA GCGGCATCTT TTTCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTAA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ACAAAAGCAA ATTATTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGGGATCCTT GCAAATTTGC ACTGTGGGTA 
GGGAGGACGC CAACTTCAGA TAACAAAATT GTCCTTAAGG CTTCCAGTAT CGAGAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAAGTA ATACAGGAAA GGACCATCCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATCCCCAAA ACAGCGCCAG CCACGAGACA GAAGGGAAGG 
AGGGATGGAG AGGACCTGGA CAGCCAAGGG GATGGCAGCA GCCAGCCTGA TACAATTTCC 
ATCGCCTCTC GGACGTCTCA GAACACATTG GATAGCGATA AGCTCTCAGG TGGCTGTGAG 
CTGACGGTTG TGATTCACGA CTTCACGGCC TGCAACAGTA ACGAGCTCAC CATCCGGCGC 
GGGCAAACAG TGGAGGTTTT GGAGCGGCCA CACGACAAGC CTGACTGGTG CCTGGTGCGC 
ACCACGGACC GGTCCCCGGC AGCGGAAGGC CTGGTGCCCT GCGGCTCTCT GTGCATTGCC 
CACTCCAGAA GTAGCATGGA GATGGAGGGC ATCTTCAACC ACAAAGACTC CCTCTCTGTC 
TCCAGTAACG ATGCCAGTCC ACCTGCCTCC GTAGCTTCCC TTCAGCCCCA CATGATCGGG 
GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA GGCAACACCT TGCGCAAGTG GCTTACCAGC 
CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT GATGGGCACG TGAAGAAGCT TGCCCACAAG 
CACAAAAAGA GCAGGGAGGT CCGCAAGAGC ACAGACGCTG GCTCGCAGAA GGACTCGGAT 
GACAGTGCAG CCACTCCTCA GGACGAGACT GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC 
AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG TCAGGCATGC AGAGCTGTGG AGAAGAAGAA 
GGGGAGGAGG GGGCCGATGC TGTGCCACTC CCCCCACCCA TGGCTATCCA GCAGCACAGC 
CTCCTTCAGC CGGACTCGCA GGATGACAAG GCCTCTTCTC GATTACTAGT CCGCCCCACC 
AGCTCCGAAA CTCCAAGCGC AGCCGAACTT GTCAGTGCAA TCGAGGAACT CGTGAAGAGC 
AAGATGGCAC TGGAGGATCG TCCTAGCTCC CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCATCGGA CAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGGCACTACG TCTTGCAAGA ATTAGTGGAG 
ACAGAGCGTG ACTATGTACG AGACCTTGGC TACGTGGTTG AAGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAG GGAAAAGACA AGATTGTGTT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA 
GATCCAGAAA AACTAGGATC ACTCTTTGTG AAACACGAGA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAACG CCTTGGCCAC AGACTGCAGC TCACAGATTT GTTAATCAAA 
CCTGTGCAGA GAATTATGAA GTACCAGCTG TTGCTGAAGG ACTTCCTTAA GTACTCCAAA 
AAAGCTAGTC TGGACACATC AGAATTAGAG AAAGCCGTGG AAGTCATGTG CATAGTCCCA 
AAGCGGTGCA ACGACATGAT GAACGTGGGG AGGCTGCAAG GATTCGACGG TAAAATCGTC 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTTTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC CTCTTTGAAC AGATTGTCAT ATTCAGTGAA 
CCACTAGACA AAAAAAAGGG TTTCTCCATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTCTGACA 
TCAAGGACAG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCAAATCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CCATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCAG TGGCAGCGGG 
GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC 
CATGGCAGTG GTCCCAGCAG CTGCAGCAGT GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC 
CGGATCCCAC AGCCTGTCAG ACACCACCCC CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTATG TCTGCTCCCA GCCCCTCACT GCCTCCTCCC 
AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC GGCCAACCCA GCAGGCTCCC CTTGAGCGGA 
GACTCAGAAG GTCCTGAGCG AGAAACGGAA CCCATCCCCA AGATGAAGGT GATGGACAGC 
CCCAGGAAAA CCTCCGGGAA CACTTCCGGT GCAAATCAAG ATGGAAATGC CAAGGACGCT 
CGGGGGAACC TGGGCCCTCT GCCCCTGAGC AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG 
AACTCTCCTC TTTCTACCAC AGTCCCTTCT CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC 
AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG AGCTCCATCC CCTCCTCCCC GGCAAGCCGG 
CCGGGCTCCT TCACTTTCCC GGGGGACAGC GACTCCCTCC AGCGGCAGAC ACATCGCCAC 
ACAGCCCCCA GCAAGGACAC GGACCGCATG AGCACGTGCT CCTCAGCCAG CGAGCAGTCC 
GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGTAGCAGCA GTAGCAACAT CTCCACCATG 
TTGGTGACAC ACGATTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG 
GTCGTTCAAA TTCTGGCCAG CAACCAGCAG AACATGTTTC TGGTGTTCCG TGCCGCCACT 
GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGCCA CACCAGTGCA 
GTCATCATGG AGAACCCCGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC 
CGTTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AAAGGGAGGG CAAGTTAGAG 
AATGGTTATC GGAAATCCCG GGAAGGGCTC AGCAACAAGG TATCTGTGAA GCTTCTCAAT 
CCCAACTACA TTTATGATGT TCCCCCAGAA TTCGTCATCC CATTGAGTGA GGTCACGTGT 
GAGACAGGGG AGACCGTTGT TCTTAGATGT CGTGTCTGTG GCCGCCCCAA GGCCTCAATC 
ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GCCACTTCAG CATCTCCTAC 
AGTGACTTGG GGGAGGCCGC TCTGAAGATC ATTGGTGTGA CCACGGAAGA TGATGGCATC 
TACACATGTA TTGCTGTGAA CGACATGGGT TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT 
CTAGGTCCAG GGAGTGATGG GATCATGGTG ACCTGGAAAG ACAACTTTGA CTCCTTCTAC 
AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA TTTGCTGTCG TTAAGAAATG TGATCAGAAA 
GGAACCAAAC GAGCAGTGGC CACAAAGTTT GTGAACAAGA AGTTGATGAA GCGTGACCAG 
GTCACCCATG AGCTTGGCAT CCTGCAGAAC CTCCAGCACC CGCTCCTCAT TGGCCTTCTC 
GACACCTTCG AGACCCCCAC CAGCTACGTC CTGGTCTTGG AGATGGCTGA CCAGGGTCGC 
CTCCTGGACT GCGTGGTACG ATGGGGAAGC CTCACTGAAG GAAAGATCAG AGCACACCTG 
GGGGAGGTTC TGGAAGCCGT CCGATACCTT CACAACTGCA GGATAGCACA CCTGGACCTA 
AAGCCTGAGA ATATTCTGGT GGATCAGAGT TTAGCCAAGC CAACAATTAA ACTGGCTGAC 
TTTGGAGATG CTGTCCAGCT CAACACGACC TACTACATCC ACCAGTTACT GGGGAACCCT 
GAGTTTGCAG CTCCTGAAAT CATTCTTGGG AACCCTGTCT CCCTGACCTC GGACACATGG 
AGTGTTGGAG TGCTCACGTA CGTGCTTCTT AGCGGTGTGT CCCCCTTCCT GGATGACAGC 
GTGGAAGAGA CCTGCCTGAA TATTTGCCGA TTAGACTTTA GTTTCCCAGA TGACTACTTT 
AAAGGAGTTA GCCAGAAGGC CAAGGAATTC GTGTGCTTCC TCCTGCAGGA GGACCCTGCC 
AAGCGTCCCT CGGCTGCACT GGCTCTCCAG GAGCAGTGGC TACAGGTGGG CAACGGCAAC 
GGCAAAGGCT CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA 
CACCAGAATG ATGTTCGACC TATTCGTAGC ATTAAAAACT TTCTACAGAG CAGGCTTCTG 
CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005336810.1
CDS	19..9150
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQELIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETAEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSNPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSSSGGGGSSGGASGGGGSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGMSAPSPSLPPPSSSLAFEASLGQPSRLPLSGDSEGPERETEPIPKMKVMDSPRKTSGNTSGANQDGNAKDARGNLGPLPLSKPRPGAISPLNSPLSTTVPSPFGKEPFPPSSPLQKGGSFWSSIPSSPASRPGSFTFPGDSDSLQRQTHRHTAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHFSISYSDLGEAALKIIGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFAVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQVGNGNGKGSGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_005336753.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005336753.3

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT GCCAATTCTG AAAGAAAAAG TTGCTTATCT TTCAGGTGGT 
AGAGATAAGC GTGGAGGTCC CATTTTAACA TTTCCAGCCC GCAGCAATCA TGACAGAATA 
CGGCAGGAGG ATCTCAGGAG ACTCATTTCA TATCTAGCCT GCATTCCTAG TGAGGAAGTC 
TGCAAGCGAG GCTTCACGGT GATTGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG 
CCCCTACTGA AGATCCTGCA GGAATCCTTC CCCTGCTGCA TCCACGTCGC CCTGATAATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACGAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAACA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTCG ATGGCTGCCT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC 
ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC CTAGAAGGGG CTCGGAATAT GATAGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAA GCCCCCATTG AGGACTTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC GATAGTTTTC CCAAAAAGAA CTCAGGGTCA 
GGTAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTAGA TAGGCTGCAC 
TCGACCCGGC AGCATCTGCA TCAGATGTGG CATGTGAGGA AGTTGAAGCT GGACCAATGC 
TTTCAGCTGA GGCTGTTTGA ACAAGATGCT GAAAAGATGT TTGACTGGAT TACACACAAC 
AAAGGCCTGT TTCTAAACAG TTACACAGAG ATTGGGACCA GCCACCCTCA TGCAATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCTATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATAATGT CGGTGGCCAA TCGCTTGGTG GAATCTGGCC ACTATGCCTC TCAGCAGATC 
AAGCAGATTG CAAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG 
CGTAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTACATGAGC 
AACGTGGACT CCTGGTGTAA AGCCTGTGGT GAAGTAGACC TTCCATCTGA GCTGCAGGAT 
CTGGAAGATG CCATCCATCA CCATCAGGGA ATCTACGAGC ACATCACCCT TGCTTATTCT 
GAGGTGAGCC AAGATGGAAA GTCACTTCTT GACAAGCTCC AGAGGCCCTT GACTCCTGGC 
AGCTCTGACT CCCTAACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTGGAT 
GTCATCCATG AGGTGCTGCA CCATCAACGG CAGCTTGAGA ACATCTGGCA ACACCGCAAA 
GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA ACAGGTGCTG 
GATTGGATCG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC 
ACATACACCA ATGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAG 
TGCGACCCAG AAGAGATTTA TCAGGCTGCC CATCAGCTTG AAGACCGGAT ACAAGATTTT 
GTTCGGCGCG TTGAGCAACG AAAGATTCTC CTGGACATGT CGGTGTCCTT TCACACCCAC 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG TTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACGCTGCAGG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATTCA GCAGCTGAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CATAACAGCT CCATCAACCA CATCGAGACG 
GTGCTGCAGC AGCTGGACGA GGCCCAGTCA CAGATGGAGG AACTCTTCCA GGAGCGCAAG 
ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC ATATTTGAGA GGGATGCCAT AGATATCATC 
TCAGACCTTG AGTCTTGGAA TGATGAGCTT TCTCAACAAA TGAATGACTT TGACACAGAA 
GATCTCACAA TTGCAGAACA GCGCCTCCAG CACCATGCAG ACAAGGCCTT GACCATGAAT 
AACTTGACTT TTGATGTCAT TCACCAAGGG CAGGATCTTC TGCAGTATGT CAATGAAGTC 
CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT AGAGATGTAG ATATGGCAAC TCGAGTCCAG 
GACCTGTTGG AGTTTCTTCA TGAAAAGCAG CAGGAGTTGG ATTTAGCTGC AGAACAGCAC 
CGGAAGCACC TGGAGCAGTG CGTGCAGCTG CGCCATCTAC AGGCAGAGGT GAAACAGGTG 
TTGGGGTGGA TACGCAATGG AGAGTCTATG TTAAATGCCG GGCTGATCAC AGCCAGTTCA 
CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG CATGAGCAGT TCCAGCATGC TATCGAGAAA 
ACGCACCAGA GTGCACTGCA GGTGCAGCAG AAGGCTGAGG CAATGCTTCA GGCCAATCAC 
TATGACATGG ACATGATCCG GGACTGTGCT GAGAAGGTGG CCTCACACTG GCAGCAGCTC 
ATGCTCAAAA TGGAAGATCG CCTCAAGCTT GTCAATGCCT CTGTTGCCTT CTACAAAACT 
TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC CTAGAGCAGG AATACAAGAG AGAGGAAGAC 
TGGTGTGGTG GAGCTGACAA GCTGGGCCCC AATTCTGAGA CAGACCATGT CACCCCGATG 
ATCAGCAAGC ACCTTGAACA AAAGGAGGCT TTCCTAAAGG CTTGCACACT TGCTAGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTACAC AGGAACAGTG TGAACATGCC AGGAATGGTG 
ACACACATCA AGGCTCCAGA GCAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TACTGCATTA TTGGACCATG AGGAAGAGAC GGCTGGACCA GTGCCAGCAG 
TATGTGGTCT TTGAGCGAAG TGCCAAGCAG GCTTTAGAAT GGATTCATGA CAACGGGGAG 
TTCTACCTTT CCACACATAC CTCAACAGGG TCCAGCATAC AACACACCCA GGAGCTTCTG 
AAAGAACATG AGGAGTTTCA GATAACTGCG AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA AATAAAAAAA 
TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAATACAGG 
ACCTCTTTGG AGAAAGCTCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTT 
CAGCTTGATA TCATCCCAGC CAGTATCCCT GGGTCAGAGG TGAAGCTTCG AGATGCTGCT 
CATGAACTCA ATGAAGAAAA ACGGAAGTCT GCCCGCAGGA AAGAGTTCAT AATGGCAGAG 
CTAATTCAAA CTGAAAAGGC TTATGTAAGA GACCTTCGGG AGTGTATGGA TACCTACCTA 
TGGGAAATGA CTAGTGGAGT GGAGGAGATC CCGCCTGGCA TTGTAAACAA AGAGCTCATT 
ATCTTTGGAA ACATGCAGGA AATCTATGAA TTTCATAATA ACATCTTCCT AAAGGAGCTG 
GAAAAATATG AACAACTGCC AGAGGATGTT GGACATTGCT TTGTTACCTG GGCAGACAAG 
TTTCAGATGT ATGTCACCTA TTGCAAAAAT AAGCCTGATT CTACTCAGCT TATATTGGAA 
CATGCAGGGT CCTACTTTGA TGAGATACAG CAGCGGCATG GATTAGCCAA TTCCATCTCT 
TCTTACCTTA TTAAACCAGT TCAGCGAATA ACAAAATATC AGCTCCTTTT GAAAGAGCTG 
CTCACGTGCT GTGAGGAAGG AAAGGGAGAA ATTAAAGACG GCCTTGAGGT GATGCTTAGT 
GTGCCAAAGC GAGCCAACGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAGCT CATCTTGCAA GAGTCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AAGGTCGAGA GCGGCATCTT TTTCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTAA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ACAAAAGCAA ATTATTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGGGATCCTT GCAAATTTGC ACTGTGGGTA 
GGGAGGACGC CAACTTCAGA TAACAAAATT GTCCTTAAGG CTTCCAGTAT CGAGAACAAG 
CAGGACTGGA TAAAGCACAT CCGCGAAGTA ATACAGGAAA GGACCATCCA CCTGAAAGGA 
GCCCTGAAGG AGCCCATTCA CATCCCCAAA ACAGCGCCAG CCACGAGACA GAAGGGAAGG 
AGGGATGGAG AGGACCTGGA CAGCCAAGGG GATGGCAGCA GCCAGCCTGA TACAATTTCC 
ATCGCCTCTC GGACGTCTCA GAACACATTG GATAGCGATA AGCTCTCAGG TGGCTGTGAG 
CTGACGGTTG TGATTCACGA CTTCACGGCC TGCAACAGTA ACGAGCTCAC CATCCGGCGC 
GGGCAAACAG TGGAGGTTTT GGAGCGGCCA CACGACAAGC CTGACTGGTG CCTGGTGCGC 
ACCACGGACC GGTCCCCGGC AGCGGAAGGC CTGGTGCCCT GCGGCTCTCT GTGCATTGCC 
CACTCCAGAA GTAGCATGGA GATGGAGGGC ATCTTCAACC ACAAAGACTC CCTCTCTGTC 
TCCAGTAACG ATGCCAGTCC ACCTGCCTCC GTAGCTTCCC TTCAGCCCCA CATGATCGGG 
GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA GGCAACACCT TGCGCAAGTG GCTTACCAGC 
CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT GATGGGCACG TGAAGAAGCT TGCCCACAAG 
CACAAAAAGA GCAGGGAGGT CCGCAAGAGC ACAGACGCTG GCTCGCAGAA GGACTCGGAT 
GACAGTGCAG CCACTCCTCA GGACGAGACT GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC 
AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG TCAGGCATGC AGAGCTGTGG AGAAGAAGAA 
GGGGAGGAGG GGGCCGATGC TGTGCCACTC CCCCCACCCA TGGCTATCCA GCAGCACAGC 
CTCCTTCAGC CGGACTCGCA GGATGACAAG GCCTCTTCTC GATTACTAGT CCGCCCCACC 
AGCTCCGAAA CTCCAAGCGC AGCCGAACTT GTCAGTGCAA TCGAGGAACT CGTGAAGAGC 
AAGATGGCAC TGGAGGATCG TCCTAGCTCC CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC 
CCCTCCTTCA ACCCATCGGA CAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGGCACTACG TCTTGCAAGA ATTAGTGGAG 
ACAGAGCGTG ACTATGTACG AGACCTTGGC TACGTGGTTG AAGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAG GGAAAAGACA AGATTGTGTT TGGCAACATC 
CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA 
GATCCAGAAA AACTAGGATC ACTCTTTGTG AAACACGAGA GAAGGTTGCA CATGTACATA 
GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAACG CCTTGGCCAC AGACTGCAGC TCACAGATTT GTTAATCAAA 
CCTGTGCAGA GAATTATGAA GTACCAGCTG TTGCTGAAGG ACTTCCTTAA GTACTCCAAA 
AAAGCTAGTC TGGACACATC AGAATTAGAG AAAGCCGTGG AAGTCATGTG CATAGTCCCA 
AAGCGGTGCA ACGACATGAT GAACGTGGGG AGGCTGCAAG GATTCGACGG TAAAATCGTC 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTTTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC CTCTTTGAAC AGATTGTCAT ATTCAGTGAA 
CCACTAGACA AAAAAAAGGG TTTCTCCATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTCTGACA 
TCAAGGACAG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCAAATCC AAGTGTCCGG 
CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCAC CCATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCAG TGGCAGCGGG 
GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC 
CATGGCAGTG GTCCCAGCAG CTGCAGCAGT GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC 
CGGATCCCAC AGCCTGTCAG ACACCACCCC CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTATG TCTGCTCCCA GCCCCTCACT GCCTCCTCCC 
AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC GGCCAACCCA GCAGGCTCCC CTTGAGCGGA 
GACTCAGAAG GTCCTGAGCG AGAAACGGAA CCCATCCCCA AGATGAAGGT GATGGACAGC 
CCCAGGAAAA CCTCCGGGAA CACTTCCGGT GCAAATCAAG ATGGAAATGC CAAGGACGCT 
CGGGGGAACC TGGGCCCTCT GCCCCTGAGC AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG 
AACTCTCCTC TTTCTACCAC AGTCCCTTCT CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC 
AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG AGCTCCATCC CCTCCTCCCC GGCAAGCCGG 
CCGGGCTCCT TCACTTTCCC GGGGGACAGC GACTCCCTCC AGCGGCAGAC ACATCGCCAC 
ACAGCCCCCA GCAAGGACAC GGACCGCATG AGCACGTGCT CCTCAGCCAG CGAGCAGTCC 
GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGTAGCAGCA GTAGCAACAT CTCCACCATG 
TTGGTGACAC ACGATTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG 
GTCGTTCAAA TTCTGGCCAG CAACCAGCAG AACATGTTTC TGGTGTTCCG TGCCGCCACT 
GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGCCA CACCAGTGCA 
GTCATCATGG AGAACCCCGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC 
CGTTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AAAGGGAGGG CAAGTTAGAG 
AATGGTTATC GGAAATCCCG GGAAGGGCTC AGCAACAAGG TATCTGTGAA GCTTCTCAAT 
CCCAACTACA TTTATGATGT TCCCCCAGAA TTCGTCATCC CATTGAGTGA GGTCACGTGT 
GAGACAGGGG AGACCGTTGT TCTTAGATGT CGTGTCTGTG GCCGCCCCAA GGCCTCAATC 
ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GCCACTTCAG CATCTCCTAC 
AGTGACTTGG GGGAGGCCGC TCTGAAGATC ATTGGTGTGA CCACGGAAGA TGATGGCATC 
TACACATGTA TTGCTGTGAA CGACATGGGT TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT 
CTAGGTCCAG GGAGTGATGG GATCATGGTG ACCTGGAAAG ACAACTTTGA CTCCTTCTAC 
AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA TTTGCTGTCG TTAAGAAATG TGATCAGAAA 
GGAACCAAAC GAGCAGTGGC CACAAAGTTT GTGAACAAGA AGTTGATGAA GCGTGACCAG 
GTCACCCATG AGCTTGGCAT CCTGCAGAAC CTCCAGCACC CGCTCCTCAT TGGCCTTCTC 
GACACCTTCG AGACCCCCAC CAGCTACGTC CTGGTCTTGG AGATGGCTGA CCAGGGTCGC 
CTCCTGGACT GCGTGGTACG ATGGGGAAGC CTCACTGAAG GAAAGATCAG AGCACACCTG 
GGGGAGGTTC TGGAAGCCGT CCGATACCTT CACAACTGCA GGATAGCACA CCTGGACCTA 
AAGCCTGAGA ATATTCTGGT GGATCAGAGT TTAGCCAAGC CAACAATTAA ACTGGCTGAC 
TTTGGAGATG CTGTCCAGCT CAACACGACC TACTACATCC ACCAGTTACT GGGGAACCCT 
GAGTTTGCAG CTCCTGAAAT CATTCTTGGG AACCCTGTCT CCCTGACCTC GGACACATGG 
AGTGTTGGAG TGCTCACGTA CGTGCTTCTT AGCGGTGTGT CCCCCTTCCT GGATGACAGC 
GTGGAAGAGA CCTGCCTGAA TATTTGCCGA TTAGACTTTA GTTTCCCAGA TGACTACTTT 
AAAGGAGTTA GCCAGAAGGC CAAGGAATTC GTGTGCTTCC TCCTGCAGGA GGACCCTGCC 
AAGCGTCCCT CGGCTGCACT GGCTCTCCAG GAGCAGTGGC TACAGGTGGG CAACGGCAAC 
GGCAAAGGCT CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA 
CACCAGAATG ATGTTCGACC TATTCGTAGC ATTAAAAACT TTCTACAGAG CAGGCTTCTG 
CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trio ( XM_021734206.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589881.1 Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X2, mRNA.

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CloneID	OIc83524
Clone ID Related Accession (Same CDS sequence)	XM_021734206.1
Accession Version	XM_021734206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	triple functional domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936665.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGGT CCAAGTGGGA CTCCATTAAG CCCCTACTGA AGATCCTGCA GGAATCCTTC 
CCCTGCTGCA TCCACGTCGC CCTGATAATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACGAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAACA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTCG ATGGCTGCCT GGAATACAAC 
CATGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCAACCCAC 
ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC 
CTAGAAGGGG CTCGGAATAT GATAGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAA 
GCCCCCATTG AGGACTTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC 
GATAGTTTTC CCAAAAAGAA CTCAGGGTCA GGTAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTAGA TAGGCTGCAC TCGACCCGGC AGCATCTGCA TCAGATGTGG 
CATGTGAGGA AGTTGAAGCT GGACCAATGC TTTCAGCTGA GGCTGTTTGA ACAAGATGCT 
GAAAAGATGT TTGACTGGAT TACACACAAC AAAGGCCTGT TTCTAAACAG TTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCAATGGAG CTTCAGACGC AGCACAATCA CTTTGCTATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATAATGT CGGTGGCCAA TCGCTTGGTG 
GAATCTGGCC ACTATGCCTC TCAGCAGATC AAGCAGATTG CAAATCAGCT GGAGCAGGAG 
TGGAAGGCAT TTGCGGCAGC CCTGGATGAG CGTAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCTGAAAA GTACATGAGC AACGTGGACT CCTGGTGTAA AGCCTGTGGT 
GAAGTAGACC TTCCATCTGA GCTGCAGGAT CTGGAAGATG CCATCCATCA CCATCAGGGA 
ATCTACGAGC ACATCACCCT TGCTTATTCT GAGGTGAGCC AAGATGGAAA GTCACTTCTT 
GACAAGCTCC AGAGGCCCTT GACTCCTGGC AGCTCTGACT CCCTAACGGC CTCTGCCAAC 
TACTCCAAGG CTGTGCACCA TGTCCTGGAT GTCATCCATG AGGTGCTGCA CCATCAACGG 
CAGCTTGAGA ACATCTGGCA ACACCGCAAA GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA ACAGGTGCTG GATTGGATCG AGAACCATGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCTTT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT AGCACAGAAC ACATACACCA ATGCAGATAA ATTACTAGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAG TGCGACCCAG AAGAGATTTA TCAGGCTGCC 
CATCAGCTTG AAGACCGGAT ACAAGATTTT GTTCGGCGCG TTGAGCAACG AAAGATTCTC 
CTGGACATGT CGGTGTCCTT TCACACCCAC GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
TTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACGCTGCAGG TGACGGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATTCA GCAGCTGAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CATAACAGCT CCATCAACCA CATCGAGACG GTGCTGCAGC AGCTGGACGA GGCCCAGTCA 
CAGATGGAGG AACTCTTCCA GGAGCGCAAG ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC 
ATATTTGAGA GGGATGCCAT AGATATCATC TCAGACCTTG AGTCTTGGAA TGATGAGCTT 
TCTCAACAAA TGAATGACTT TGACACAGAA GATCTCACAA TTGCAGAACA GCGCCTCCAG 
CACCATGCAG ACAAGGCCTT GACCATGAAT AACTTGACTT TTGATGTCAT TCACCAAGGG 
CAGGATCTTC TGCAGTATGT CAATGAAGTC CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT 
AGAGATGTAG ATATGGCAAC TCGAGTCCAG GACCTGTTGG AGTTTCTTCA TGAAAAGCAG 
CAGGAGTTGG ATTTAGCTGC AGAACAGCAC CGGAAGCACC TGGAGCAGTG CGTGCAGCTG 
CGCCATCTAC AGGCAGAGGT GAAACAGGTG TTGGGGTGGA TACGCAATGG AGAGTCTATG 
TTAAATGCCG GGCTGATCAC AGCCAGTTCA CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG 
CATGAGCAGT TCCAGCATGC TATCGAGAAA ACGCACCAGA GTGCACTGCA GGTGCAGCAG 
AAGGCTGAGG CAATGCTTCA GGCCAATCAC TATGACATGG ACATGATCCG GGACTGTGCT 
GAGAAGGTGG CCTCACACTG GCAGCAGCTC ATGCTCAAAA TGGAAGATCG CCTCAAGCTT 
GTCAATGCCT CTGTTGCCTT CTACAAAACT TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC 
CTAGAGCAGG AATACAAGAG AGAGGAAGAC TGGTGTGGTG GAGCTGACAA GCTGGGCCCC 
AATTCTGAGA CAGACCATGT CACCCCGATG ATCAGCAAGC ACCTTGAACA AAAGGAGGCT 
TTCCTAAAGG CTTGCACACT TGCTAGGAGG AATGCAGACG TCTTCCTGAA ATACCTACAC 
AGGAACAGTG TGAACATGCC AGGAATGGTG ACACACATCA AGGCTCCAGA GCAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TACTGCATTA TTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGCCAGCAG TATGTGGTCT TTGAGCGAAG TGCCAAGCAG 
GCTTTAGAAT GGATTCATGA CAACGGGGAG TTCTACCTTT CCACACATAC CTCAACAGGG 
TCCAGCATAC AACACACCCA GGAGCTTCTG AAAGAACATG AGGAGTTTCA GATAACTGCG 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA AATAAAAAAA TGTGTCACTG CAGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAATACAGG ACCTCTTTGG AGAAAGCTCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTT CAGCTTGATA TCATCCCAGC CAGTATCCCT 
GGGTCAGAGG TGAAGCTTCG AGATGCTGCT CATGAACTCA ATGAAGAAAA ACGGAAGTCT 
GCCCGCAGGA AAGAGTTCAT AATGGCAGAG CTAATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTTCGGG AGTGTATGGA TACCTACCTA TGGGAAATGA CTAGTGGAGT GGAGGAGATC 
CCGCCTGGCA TTGTAAACAA AGAGCTCATT ATCTTTGGAA ACATGCAGGA AATCTATGAA 
TTTCATAATA ACATCTTCCT AAAGGAGCTG GAAAAATATG AACAACTGCC AGAGGATGTT 
GGACATTGCT TTGTTACCTG GGCAGACAAG TTTCAGATGT ATGTCACCTA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT TATATTGGAA CATGCAGGGT CCTACTTTGA TGAGATACAG 
CAGCGGCATG GATTAGCCAA TTCCATCTCT TCTTACCTTA TTAAACCAGT TCAGCGAATA 
ACAAAATATC AGCTCCTTTT GAAAGAGCTG CTCACGTGCT GTGAGGAAGG AAAGGGAGAA 
ATTAAAGACG GCCTTGAGGT GATGCTTAGT GTGCCAAAGC GAGCCAACGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAGCT CATCTTGCAA 
GAGTCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AAGGTCGAGA GCGGCATCTT 
TTTCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTAA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ACAAAAGCAA ATTATTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGGGATCCTT GCAAATTTGC ACTGTGGGTA GGGAGGACGC CAACTTCAGA TAACAAAATT 
GTCCTTAAGG CTTCCAGTAT CGAGAACAAG CAGGACTGGA TAAAGCACAT CCGCGAAGTA 
ATACAGGAAA GGACCATCCA CCTGAAAGGA GCCCTGAAGG AGCCCATTCA CATCCCCAAA 
ACAGCGCCAG CCACGAGACA GAAGGGAAGG AGGGATGGAG AGGACCTGGA CAGCCAAGGG 
GATGGCAGCA GCCAGCCTGA TACAATTTCC ATCGCCTCTC GGACGTCTCA GAACACATTG 
GATAGCGATA AGCTCTCAGG TGGCTGTGAG CTGACGGTTG TGATTCACGA CTTCACGGCC 
TGCAACAGTA ACGAGCTCAC CATCCGGCGC GGGCAAACAG TGGAGGTTTT GGAGCGGCCA 
CACGACAAGC CTGACTGGTG CCTGGTGCGC ACCACGGACC GGTCCCCGGC AGCGGAAGGC 
CTGGTGCCCT GCGGCTCTCT GTGCATTGCC CACTCCAGAA GTAGCATGGA GATGGAGGGC 
ATCTTCAACC ACAAAGACTC CCTCTCTGTC TCCAGTAACG ATGCCAGTCC ACCTGCCTCC 
GTAGCTTCCC TTCAGCCCCA CATGATCGGG GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA 
GGCAACACCT TGCGCAAGTG GCTTACCAGC CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT 
GATGGGCACG TGAAGAAGCT TGCCCACAAG CACAAAAAGA GCAGGGAGGT CCGCAAGAGC 
ACAGACGCTG GCTCGCAGAA GGACTCGGAT GACAGTGCAG CCACTCCTCA GGACGAGACT 
GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG 
TCAGGCATGC AGAGCTGTGG AGAAGAAGAA GGGGAGGAGG GGGCCGATGC TGTGCCACTC 
CCCCCACCCA TGGCTATCCA GCAGCACAGC CTCCTTCAGC CGGACTCGCA GGATGACAAG 
GCCTCTTCTC GATTACTAGT CCGCCCCACC AGCTCCGAAA CTCCAAGCGC AGCCGAACTT 
GTCAGTGCAA TCGAGGAACT CGTGAAGAGC AAGATGGCAC TGGAGGATCG TCCTAGCTCC 
CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC CCCTCCTTCA ACCCATCGGA CAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGGCACTACG TCTTGCAAGA ATTAGTGGAG ACAGAGCGTG ACTATGTACG AGACCTTGGC 
TACGTGGTTG AAGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAG 
GGAAAAGACA AGATTGTGTT TGGCAACATC CATCAGATTT ATGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA GATCCAGAAA AACTAGGATC ACTCTTTGTG 
AAACACGAGA GAAGGTTGCA CATGTACATA GTTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAACG CCTTGGCCAC 
AGACTGCAGC TCACAGATTT GTTAATCAAA CCTGTGCAGA GAATTATGAA GTACCAGCTG 
TTGCTGAAGG ACTTCCTTAA GTACTCCAAA AAAGCTAGTC TGGACACATC AGAATTAGAG 
AAAGCCGTGG AAGTCATGTG CATAGTCCCA AAGCGGTGCA ACGACATGAT GAACGTGGGG 
AGGCTGCAAG GATTCGACGG TAAAATCGTC GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTTTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC 
CTCTTTGAAC AGATTGTCAT ATTCAGTGAA CCACTAGACA AAAAAAAGGG TTTCTCCATG 
CCAGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CATGTAAATT TGCTCTGACA TCAAGGACAG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCAAATCC AAGTGTCCGG CAAACATGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCAC CCATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCAG TGGCAGCGGG GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC 
GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC CATGGCAGTG GTCCCAGCAG CTGCAGCAGT 
GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC CGGATCCCAC AGCCTGTCAG ACACCACCCC 
CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTATG 
TCTGCTCCCA GCCCCTCACT GCCTCCTCCC AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC 
GGCCAACCCA GCAGGCTCCC CTTGAGCGGA GACTCAGAAG GTCCTGAGCG AGAAACGGAA 
CCCATCCCCA AGATGAAGGT GATGGACAGC CCCAGGAAAA CCTCCGGGAA CACTTCCGGT 
GCAAATCAAG ATGGAAATGC CAAGGACGCT CGGGGGAACC TGGGCCCTCT GCCCCTGAGC 
AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG AACTCTCCTC TTTCTACCAC AGTCCCTTCT 
CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCTCCTCCCC GGCAAGCCGG CCGGGCTCCT TCACTTTCCC GGGGGACAGC 
GACTCCCTCC AGCGGCAGAC ACATCGCCAC ACAGCCCCCA GCAAGGACAC GGACCGCATG 
AGCACGTGCT CCTCAGCCAG CGAGCAGTCC GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAACCAGCAG 
AACATGTTTC TGGTGTTCCG TGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCGGGCTTCG TCCTGGGCCA CACCAGTGCA GTCATCATGG AGAACCCCGA CGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AAAGGGAGGG CAAGTTAGAG AATGGTTATC GGAAATCCCG GGAAGGGCTC 
AGCAACAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TTTATGATGT TCCCCCAGAA 
TTCGTCATCC CATTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TCTTAGATGT 
CGTGTCTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAACA CAACACCTTG 
AACAACGACG GCCACTTCAG CATCTCCTAC AGTGACTTGG GGGAGGCCGC TCTGAAGATC 
ATTGGTGTGA CCACGGAAGA TGATGGCATC TACACATGTA TTGCTGTGAA CGACATGGGT 
TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT CTAGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTTGCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACAAAGTTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AGCTTGGCAT CCTGCAGAAC 
CTCCAGCACC CGCTCCTCAT TGGCCTTCTC GACACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTCTTGG AGATGGCTGA CCAGGGTCGC CTCCTGGACT GCGTGGTACG ATGGGGAAGC 
CTCACTGAAG GAAAGATCAG AGCACACCTG GGGGAGGTTC TGGAAGCCGT CCGATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAGA ATATTCTGGT GGATCAGAGT 
TTAGCCAAGC CAACAATTAA ACTGGCTGAC TTTGGAGATG CTGTCCAGCT CAACACGACC 
TACTACATCC ACCAGTTACT GGGGAACCCT GAGTTTGCAG CTCCTGAAAT CATTCTTGGG 
AACCCTGTCT CCCTGACCTC GGACACATGG AGTGTTGGAG TGCTCACGTA CGTGCTTCTT 
AGCGGTGTGT CCCCCTTCCT GGATGACAGC GTGGAAGAGA CCTGCCTGAA TATTTGCCGA 
TTAGACTTTA GTTTCCCAGA TGACTACTTT AAAGGAGTTA GCCAGAAGGC CAAGGAATTC 
GTGTGCTTCC TCCTGCAGGA GGACCCTGCC AAGCGTCCCT CGGCTGCACT GGCTCTCCAG 
GAGCAGTGGC TACAGGTGGG CAACGGCAAC GGCAAAGGCT CGGGCGTCCT CGACACGTCC 
AGACTGACCT CCTTCATTGA GCGGCGCAAA CACCAGAATG ATGTTCGACC TATTCGTAGC 
ATTAAAAACT TTCTACAGAG CAGGCTTCTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589881.1
CDS	738..9659
Translation	MRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQELIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSTDAGSQKDSDDSAATPQDETAEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELEKAVEVMCIVPKRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSNPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSSSGGGGSSGGASGGGGSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGMSAPSPSLPPPSSSLAFEASLGQPSRLPLSGDSEGPERETEPIPKMKVMDSPRKTSGNTSGANQDGNAKDARGNLGPLPLSKPRPGAISPLNSPLSTTVPSPFGKEPFPPSSPLQKGGSFWSSIPSSPASRPGSFTFPGDSDSLQRQTHRHTAPSKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHFSISYSDLGEAALKIIGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGSDGIMVTWKDNFDSFYSEVAELGRGRFAVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQNLQHPLLIGLLDTFETPTSYVLVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQVGNGNGKGSGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_021734206.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus trio Rho guanine nucleotide exchange factor (Trio), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734206.1

ORF Insert Sequence:

ATGCGTGGGT CCAAGTGGGA CTCCATTAAG CCCCTACTGA AGATCCTGCA GGAATCCTTC 
CCCTGCTGCA TCCACGTCGC CCTGATAATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACGAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAACA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTCG ATGGCTGCCT GGAATACAAC 
CATGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCAACCCAC 
ATGCTGTCCC GGCTAGAGGA ACTTCAGGAC ATCCTTGCTA AGAAGGAGTT GCCTCAGGAC 
CTAGAAGGGG CTCGGAATAT GATAGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAA 
GCCCCCATTG AGGACTTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGAAT TCAGAGCAGC 
GATAGTTTTC CCAAAAAGAA CTCAGGGTCA GGTAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTAGA TAGGCTGCAC TCGACCCGGC AGCATCTGCA TCAGATGTGG 
CATGTGAGGA AGTTGAAGCT GGACCAATGC TTTCAGCTGA GGCTGTTTGA ACAAGATGCT 
GAAAAGATGT TTGACTGGAT TACACACAAC AAAGGCCTGT TTCTAAACAG TTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCAATGGAG CTTCAGACGC AGCACAATCA CTTTGCTATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATAATGT CGGTGGCCAA TCGCTTGGTG 
GAATCTGGCC ACTATGCCTC TCAGCAGATC AAGCAGATTG CAAATCAGCT GGAGCAGGAG 
TGGAAGGCAT TTGCGGCAGC CCTGGATGAG CGTAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCTGAAAA GTACATGAGC AACGTGGACT CCTGGTGTAA AGCCTGTGGT 
GAAGTAGACC TTCCATCTGA GCTGCAGGAT CTGGAAGATG CCATCCATCA CCATCAGGGA 
ATCTACGAGC ACATCACCCT TGCTTATTCT GAGGTGAGCC AAGATGGAAA GTCACTTCTT 
GACAAGCTCC AGAGGCCCTT GACTCCTGGC AGCTCTGACT CCCTAACGGC CTCTGCCAAC 
TACTCCAAGG CTGTGCACCA TGTCCTGGAT GTCATCCATG AGGTGCTGCA CCATCAACGG 
CAGCTTGAGA ACATCTGGCA ACACCGCAAA GTCCGGCTGC ACCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA ACAGGTGCTG GATTGGATCG AGAACCATGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCTTT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT AGCACAGAAC ACATACACCA ATGCAGATAA ATTACTAGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAG TGCGACCCAG AAGAGATTTA TCAGGCTGCC 
CATCAGCTTG AAGACCGGAT ACAAGATTTT GTTCGGCGCG TTGAGCAACG AAAGATTCTC 
CTGGACATGT CGGTGTCCTT TCACACCCAC GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
TTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCTGAGTCCG TGGAAGCCGT GCAGGAGCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACGCTGCAGG TGACGGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATTCA GCAGCTGAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CATAACAGCT CCATCAACCA CATCGAGACG GTGCTGCAGC AGCTGGACGA GGCCCAGTCA 
CAGATGGAGG AACTCTTCCA GGAGCGCAAG ATCAAGCTTG AGCTCTTCCT GCAGCTGCGC 
ATATTTGAGA GGGATGCCAT AGATATCATC TCAGACCTTG AGTCTTGGAA TGATGAGCTT 
TCTCAACAAA TGAATGACTT TGACACAGAA GATCTCACAA TTGCAGAACA GCGCCTCCAG 
CACCATGCAG ACAAGGCCTT GACCATGAAT AACTTGACTT TTGATGTCAT TCACCAAGGG 
CAGGATCTTC TGCAGTATGT CAATGAAGTC CAGGCCTCTG GCGTGGAGCT GCTTTGTGAT 
AGAGATGTAG ATATGGCAAC TCGAGTCCAG GACCTGTTGG AGTTTCTTCA TGAAAAGCAG 
CAGGAGTTGG ATTTAGCTGC AGAACAGCAC CGGAAGCACC TGGAGCAGTG CGTGCAGCTG 
CGCCATCTAC AGGCAGAGGT GAAACAGGTG TTGGGGTGGA TACGCAATGG AGAGTCTATG 
TTAAATGCCG GGCTGATCAC AGCCAGTTCA CTGCAGGAGG CAGAGCAGCT GCAGAGAGAG 
CATGAGCAGT TCCAGCATGC TATCGAGAAA ACGCACCAGA GTGCACTGCA GGTGCAGCAG 
AAGGCTGAGG CAATGCTTCA GGCCAATCAC TATGACATGG ACATGATCCG GGACTGTGCT 
GAGAAGGTGG CCTCACACTG GCAGCAGCTC ATGCTCAAAA TGGAAGATCG CCTCAAGCTT 
GTCAATGCCT CTGTTGCCTT CTACAAAACT TCAGAGCAGG TCTGCAGTGT CCTAGAGAGC 
CTAGAGCAGG AATACAAGAG AGAGGAAGAC TGGTGTGGTG GAGCTGACAA GCTGGGCCCC 
AATTCTGAGA CAGACCATGT CACCCCGATG ATCAGCAAGC ACCTTGAACA AAAGGAGGCT 
TTCCTAAAGG CTTGCACACT TGCTAGGAGG AATGCAGACG TCTTCCTGAA ATACCTACAC 
AGGAACAGTG TGAACATGCC AGGAATGGTG ACACACATCA AGGCTCCAGA GCAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TACTGCATTA TTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGCCAGCAG TATGTGGTCT TTGAGCGAAG TGCCAAGCAG 
GCTTTAGAAT GGATTCATGA CAACGGGGAG TTCTACCTTT CCACACATAC CTCAACAGGG 
TCCAGCATAC AACACACCCA GGAGCTTCTG AAAGAACATG AGGAGTTTCA GATAACTGCG 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA AATAAAAAAA TGTGTCACTG CAGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAATACAGG ACCTCTTTGG AGAAAGCTCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTT CAGCTTGATA TCATCCCAGC CAGTATCCCT 
GGGTCAGAGG TGAAGCTTCG AGATGCTGCT CATGAACTCA ATGAAGAAAA ACGGAAGTCT 
GCCCGCAGGA AAGAGTTCAT AATGGCAGAG CTAATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTTCGGG AGTGTATGGA TACCTACCTA TGGGAAATGA CTAGTGGAGT GGAGGAGATC 
CCGCCTGGCA TTGTAAACAA AGAGCTCATT ATCTTTGGAA ACATGCAGGA AATCTATGAA 
TTTCATAATA ACATCTTCCT AAAGGAGCTG GAAAAATATG AACAACTGCC AGAGGATGTT 
GGACATTGCT TTGTTACCTG GGCAGACAAG TTTCAGATGT ATGTCACCTA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT TATATTGGAA CATGCAGGGT CCTACTTTGA TGAGATACAG 
CAGCGGCATG GATTAGCCAA TTCCATCTCT TCTTACCTTA TTAAACCAGT TCAGCGAATA 
ACAAAATATC AGCTCCTTTT GAAAGAGCTG CTCACGTGCT GTGAGGAAGG AAAGGGAGAA 
ATTAAAGACG GCCTTGAGGT GATGCTTAGT GTGCCAAAGC GAGCCAACGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAGCT CATCTTGCAA 
GAGTCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AAGGTCGAGA GCGGCATCTT 
TTTCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTAA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ACAAAAGCAA ATTATTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGGGATCCTT GCAAATTTGC ACTGTGGGTA GGGAGGACGC CAACTTCAGA TAACAAAATT 
GTCCTTAAGG CTTCCAGTAT CGAGAACAAG CAGGACTGGA TAAAGCACAT CCGCGAAGTA 
ATACAGGAAA GGACCATCCA CCTGAAAGGA GCCCTGAAGG AGCCCATTCA CATCCCCAAA 
ACAGCGCCAG CCACGAGACA GAAGGGAAGG AGGGATGGAG AGGACCTGGA CAGCCAAGGG 
GATGGCAGCA GCCAGCCTGA TACAATTTCC ATCGCCTCTC GGACGTCTCA GAACACATTG 
GATAGCGATA AGCTCTCAGG TGGCTGTGAG CTGACGGTTG TGATTCACGA CTTCACGGCC 
TGCAACAGTA ACGAGCTCAC CATCCGGCGC GGGCAAACAG TGGAGGTTTT GGAGCGGCCA 
CACGACAAGC CTGACTGGTG CCTGGTGCGC ACCACGGACC GGTCCCCGGC AGCGGAAGGC 
CTGGTGCCCT GCGGCTCTCT GTGCATTGCC CACTCCAGAA GTAGCATGGA GATGGAGGGC 
ATCTTCAACC ACAAAGACTC CCTCTCTGTC TCCAGTAACG ATGCCAGTCC ACCTGCCTCC 
GTAGCTTCCC TTCAGCCCCA CATGATCGGG GCCCAGAGCT CTCCGGGTCC CAAGCGGCCA 
GGCAACACCT TGCGCAAGTG GCTTACCAGC CCAGTGCGGC GGCTGAGCAG TGGCAAGGCT 
GATGGGCACG TGAAGAAGCT TGCCCACAAG CACAAAAAGA GCAGGGAGGT CCGCAAGAGC 
ACAGACGCTG GCTCGCAGAA GGACTCGGAT GACAGTGCAG CCACTCCTCA GGACGAGACT 
GCTGAAGAGA GAGGCCGGAA TGAGGGCCTC AGCAGCGGCA CGCTCTCTAA ATCCTCTTCG 
TCAGGCATGC AGAGCTGTGG AGAAGAAGAA GGGGAGGAGG GGGCCGATGC TGTGCCACTC 
CCCCCACCCA TGGCTATCCA GCAGCACAGC CTCCTTCAGC CGGACTCGCA GGATGACAAG 
GCCTCTTCTC GATTACTAGT CCGCCCCACC AGCTCCGAAA CTCCAAGCGC AGCCGAACTT 
GTCAGTGCAA TCGAGGAACT CGTGAAGAGC AAGATGGCAC TGGAGGATCG TCCTAGCTCC 
CTCCTCGTTG ACCAGGGAGA CAGCAGCAGC CCCTCCTTCA ACCCATCGGA CAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGGCACTACG TCTTGCAAGA ATTAGTGGAG ACAGAGCGTG ACTATGTACG AGACCTTGGC 
TACGTGGTTG AAGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAG 
GGAAAAGACA AGATTGTGTT TGGCAACATC CATCAGATTT ATGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTCGAA GATCCAGAAA AACTAGGATC ACTCTTTGTG 
AAACACGAGA GAAGGTTGCA CATGTACATA GTTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAACG CCTTGGCCAC 
AGACTGCAGC TCACAGATTT GTTAATCAAA CCTGTGCAGA GAATTATGAA GTACCAGCTG 
TTGCTGAAGG ACTTCCTTAA GTACTCCAAA AAAGCTAGTC TGGACACATC AGAATTAGAG 
AAAGCCGTGG AAGTCATGTG CATAGTCCCA AAGCGGTGCA ACGACATGAT GAACGTGGGG 
AGGCTGCAAG GATTCGACGG TAAAATCGTC GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTTTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAAAGAGAG GCGAGTTTTC 
CTCTTTGAAC AGATTGTCAT ATTCAGTGAA CCACTAGACA AAAAAAAGGG TTTCTCCATG 
CCAGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CATGTAAATT TGCTCTGACA TCAAGGACAG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCAAATCC AAGTGTCCGG CAAACATGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCAC CCATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCAG TGGCAGCGGG GGCAGCAGCA GCGGGGGTGG GGGCAGCAGC 
GGCGGGGCCT CCGGTGGTGG TGGCAGCAGC CATGGCAGTG GTCCCAGCAG CTGCAGCAGT 
GGCCCCAGCT CAAGCAGAAG CAGGCCCTCC CGGATCCCAC AGCCTGTCAG ACACCACCCC 
CCTGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTATG 
TCTGCTCCCA GCCCCTCACT GCCTCCTCCC AGCAGCAGCC TGGCCTTTGA GGCCAGCCTC 
GGCCAACCCA GCAGGCTCCC CTTGAGCGGA GACTCAGAAG GTCCTGAGCG AGAAACGGAA 
CCCATCCCCA AGATGAAGGT GATGGACAGC CCCAGGAAAA CCTCCGGGAA CACTTCCGGT 
GCAAATCAAG ATGGAAATGC CAAGGACGCT CGGGGGAACC TGGGCCCTCT GCCCCTGAGC 
AAGCCCAGAC CTGGGGCCAT CTCGCCTCTG AACTCTCCTC TTTCTACCAC AGTCCCTTCT 
CCTTTTGGAA AGGAGCCCTT TCCCCCCAGC AGCCCCCTAC AGAAGGGGGG CTCCTTCTGG 
AGCTCCATCC CCTCCTCCCC GGCAAGCCGG CCGGGCTCCT TCACTTTCCC GGGGGACAGC 
GACTCCCTCC AGCGGCAGAC ACATCGCCAC ACAGCCCCCA GCAAGGACAC GGACCGCATG 
AGCACGTGCT CCTCAGCCAG CGAGCAGTCC GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA 
AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG 
GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAACCAGCAG 
AACATGTTTC TGGTGTTCCG TGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT 
CCGGGCTTCG TCCTGGGCCA CACCAGTGCA GTCATCATGG AGAACCCCGA CGGGACTCTC 
AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT 
AAAGACGGCA AAAGGGAGGG CAAGTTAGAG AATGGTTATC GGAAATCCCG GGAAGGGCTC 
AGCAACAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TTTATGATGT TCCCCCAGAA 
TTCGTCATCC CATTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TCTTAGATGT 
CGTGTCTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAACA CAACACCTTG 
AACAACGACG GCCACTTCAG CATCTCCTAC AGTGACTTGG GGGAGGCCGC TCTGAAGATC 
ATTGGTGTGA CCACGGAAGA TGATGGCATC TACACATGTA TTGCTGTGAA CGACATGGGT 
TCAGCCTCAT CTTCAGCCAG TCTGAGGGTT CTAGGTCCAG GGAGTGATGG GATCATGGTG 
ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGTGAAGTGG CTGAGCTTGG CAGGGGCAGA 
TTTGCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACAAAGTTT 
GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AGCTTGGCAT CCTGCAGAAC 
CTCCAGCACC CGCTCCTCAT TGGCCTTCTC GACACCTTCG AGACCCCCAC CAGCTACGTC 
CTGGTCTTGG AGATGGCTGA CCAGGGTCGC CTCCTGGACT GCGTGGTACG ATGGGGAAGC 
CTCACTGAAG GAAAGATCAG AGCACACCTG GGGGAGGTTC TGGAAGCCGT CCGATACCTT 
CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAGA ATATTCTGGT GGATCAGAGT 
TTAGCCAAGC CAACAATTAA ACTGGCTGAC TTTGGAGATG CTGTCCAGCT CAACACGACC 
TACTACATCC ACCAGTTACT GGGGAACCCT GAGTTTGCAG CTCCTGAAAT CATTCTTGGG 
AACCCTGTCT CCCTGACCTC GGACACATGG AGTGTTGGAG TGCTCACGTA CGTGCTTCTT 
AGCGGTGTGT CCCCCTTCCT GGATGACAGC GTGGAAGAGA CCTGCCTGAA TATTTGCCGA 
TTAGACTTTA GTTTCCCAGA TGACTACTTT AAAGGAGTTA GCCAGAAGGC CAAGGAATTC 
GTGTGCTTCC TCCTGCAGGA GGACCCTGCC AAGCGTCCCT CGGCTGCACT GGCTCTCCAG 
GAGCAGTGGC TACAGGTGGG CAACGGCAAC GGCAAAGGCT CGGGCGTCCT CGACACGTCC 
AGACTGACCT CCTTCATTGA GCGGCGCAAA CACCAGAATG ATGTTCGACC TATTCGTAGC 
ATTAAAAACT TTCTACAGAG CAGGCTTCTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Trio cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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