Hectd4 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hectd4
Entrez Gene ID	101959752
Full Name	HECT domain E3 ubiquitin protein ligase 4
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021734250.1	XP_021589925.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X1
XM_021734252.1	XP_021589927.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X3
XM_013363567.1	XP_013219021.1	probable E3 ubiquitin-protein ligase HECTD4
XM_013363567.2	XP_013219021.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X6
XM_021734251.1	XP_021589926.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X2
XM_021734254.1	XP_021589929.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X5
XM_021734255.1	XP_021589930.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X7
XM_021734253.1	XP_021589928.1	probable E3 ubiquitin-protein ligase HECTD4 isoform X4

Pathways from BioSystems
Gene Ontology

The following Hectd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hectd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc67628	XM_013363567.2 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83553	XM_021734250.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83554	XM_021734251.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83555	XM_021734252.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83556	XM_021734253.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83557	XM_021734254.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OIc83558	XM_021734255.1 Latest version!	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OIc67628	XM_013363567.1	Ictidomys tridecemlineatus HECT domain containing E3 ubiquitin protein ligase 4 (Hectd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hectd4 ( XM_013363567.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013219021.1 Ictidomys tridecemlineatus HECT domain containing E3 ubiquitin protein ligase 4 (Hectd4), mRNA.

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CloneID	OIc67628
Clone ID Related Accession (Same CDS sequence)	XM_013363567.1 , XM_013363567.2
Accession Version	XM_013363567.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT TGGTGATATA TGCGGGGCCT AACACAAACA GTAGGAAGGT TGCTGAATAT 
GGAGGCAATA CTCTGGGATA TGGCAGCCGT AGTGTCTTAG GAACTGGTTG GCCGAAGGAC 
TTGGTGAAGG TGGAAGGAGA TACAGTCACC TTCTCCTTTG AAATGAGAAG TGGCCGTGAA 
CACAACACTC CTGATAAAGC TATGTGGGGC TTTGCGTGCA CAGTTCGCGC TCAGGAGTCC 
TCGGAGGACG TCTCAGGAGG CCTGCCTTTT CTGGTGGACC TGGCTTTAGG TCTGTCTGTG 
TTAGCTTGTT CCATGTTAAG AATCCTGTAC AATGGACCAG AAATTACCAA AGAAGAGGAA 
GCCTGTCAGG AGCTTTTGCG GTCCAAACTT TTACAAAGGT GCCAGTGGCA GGTGGAGGCC 
AATGGCGTCA TCTCGCCTGC CCTCACCCCA AGTCCCTCTC CACTGCCTCT GACCATAGAG 
GAAGACCGAG AGTTCACCTA CCCCTCGGAT GTCCTCGTGC CTCCTGTTGG GAACTACTTT 
GACCTGCCCC GGATCCGGCT GCCTCCAGGA ATCATGATAA AGCTCAGGGA AATTTCTGGG 
CGAGCTAGAC CTCAATTTAG ACCAAGTATA AAGGAAGTGA TTCAGCCAGA TGTGATGGAG 
GAAATGGTGG TATCATGTGT TATTAAGCAC TTGAACTTGG TCGATGCACT GCAATCCCTA 
ATAAATTTCC AATATCAAGA AGAACATGCT GAGGAATATG ACTTACTATG TAAAATTATG 
GGAGAGACCT TTAAGAAACT CAATGCCATG GAGAGACAGC TGCAGAGTGT TGCAGAACTG 
GAACAGAAGT GGCAAAGTGA GGTTGATGAT GCCATGCAGG GGAAATTGGA GAACAACATG 
CCTTTCTTTT ATGATTACCA TTTTAATGAG AACAAAATGA AAGAACTAGA ACTTTTGTGT 
TCAATGAAGG AAGTCTCCTT TGATGGAAAT GATCTTGAAA ACATGGTCCT ATCACTGAGG 
GAGAAGTTCC TACAAGAAGT GAATTCTCTT ATTCAGAAGC CCTCTCATCC ACTGGCTAAA 
ACGAAGACAT TAGTGAAGAG TCTAATGAAC CGTGCTGAGC TGCTGCTGCA TGTCACCATT 
GCGGCCCAAT CTGGCCTCAC CAGGAGCATC TCGGGAACCC CTGCAGAGAC ACCGGCTTGT 
AAATCAGCTT CTGAAACAAA GGTGATATCT CATGCCGTCC GGCAGCCTGT TTTTCTCCGT 
AGTATGTCGG CTCCTTCTGA TCTAGAAATG ATTGGTAATG AAGATTTGGA ATTTACTAGA 
GCAAGTCAGA GGCGTCGACA TGTGACCAGC CACCGGAGCA GCTCCTTTAC ACTCCTGCAA 
TCGTTGGCCA TTGAGGACAG CAGGGACAAG CCCACCTATA GTGTCCTGCT GGGGCAGCTG 
TTTGCTTTCA TTGGCACCAA TCCCGACCAA GCTGTGTCCA GCAGCAGCTT CCTTCTTGCT 
GCACAGACGA GGTGGCGGAG GGGAAACACA CGCAAGCAGG CCCTGGTGCA CATGCGAGAG 
CTGCTCACAG CTGCCGTGCG CGTTGGGGGA GTGACACATC TTGTGGGGCC AGTGACTATG 
GTCCTCCAGG GAGGACCCAG GATTGAAGAG CTCACCTGTG GTGGGATGGT TGAGCAGGTT 
CAGGAAGCCT TTGGCGAAAC CATGACTTCG GTTGTGTCTC TGTGTGCCCG TTACCCTATC 
GCTTGTGCAA ATAGCATTGG ACTCTTGTGT ACCATACCTT ATACCAGGAG TGAGGAGAAG 
TGCCTGGTGC GCAGTGGCCT TGTCCAGCTC ATGGATCGAC TGTGCAGCTT GAGCAGTCAG 
ACCGAGTCCA GCTCCAGTGA GAAACAAACC AAGAAGCAGA AAGTGGCCAC CATGGCCTGG 
GCTGCCTTCC AGGTGCTGGC CAACCGCTGT GTCGAGTGGG AAAAAGAGGA AGGTGGCTCC 
ACAGAGGCTG TGCACTCGGG GCTGGCTCGG CAGGTGTCCA GCCTTCTCAC GAATCATCTT 
GCCCGAGCCA CTGAGTGCTG TGGCAACCAG GCTGCAGGAA ATGATGCTCT GCAGGATGTC 
CTGAGTCTGC TCAACGACCT CTCGAGGAGC CACATAGGGA AAGCCATCCT GAGCCAGCCA 
GCTTGTGTGT CCAAGCTCCT CTCCCTGCTG CTTGACCAGC GCCCATCTCC AAAATTGGTC 
CTTATCATTC TTCAACTGTG CCGGGCGGCA CTGCCATTGA TGAGCGTAGA GGACTGTGGA 
AATGTGGAAC TCCCGCCATG GAGCTATTCT GTCCCCTCCC TAAACAGTGA GCAGGAGGAT 
CCCAGTGACC CAGCTTCCAA GATCGCCTCC TTGCTCTTAG CAAAGCTGGC AGATTACGTG 
GTCCCAGGAT GTCAGACAGT TCTTTCTCCA ACTGCTTCTG AACCGGATAC CACATTAACA 
AAAACCAGTC CCAAGAATTC TTTAAAAGGA GATAAAGATC CTGGAGAAGA GAGTGAAGCA 
GTGGACGGCA AGCTCTCCAT ATTTATCCAC AAACGGGAAG ACCAGTCCTC CCACGAAGTT 
CTCCAACCAT TACTAAGTAG CTCAGAAGGG CGGCCCTTCC GACTTGGTAC TGGTGCCAAC 
ATGGAGAAAG TTGTGAAGAT GGATAGAGAC ATGACCAAGG GTGGCTGTTG CGAAGTGATC 
ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA GCCACCAAGT GGGCACAGTC AGGCCTCATC 
GTCAGCATTG GGCCACCAGT AGAGTCTGTC AATCCAGAGA CTGTGAGTGG ACTATCCACA 
GGTGACAAAA AGAAAACTGC CCAAACCTCC ATCTGCAGAG AGAGGAACTC AGAACTCGCC 
AGGACTGACC CTGTCCGACC CTTCATCAGT GGGCATGTGG CAAACAGCAT GGCTGCAGAA 
GTGATCGCTT TGCTTCATAG CTTGCTAATG GCACCTGAAT CTAACGCGGC TCAAATATGG 
ACCACAACAG CAGAAAAGGT TCTGTCTCGT GCATTGATGT ACATTCCACA ACTGGGGAAA 
CATGCAGAAA GCATTCTGGA AAATGGCAGC AGCAGTGGCA GAAAACTTGC CAAACTTCAG 
AGAATTGCTC GCCAGGCTGT CGCTGCACTG TGTGCACTTG GAGGCTTCAA AGAGACAATC 
AAGATAGGAT CTGAGGTTCA GGTTTTAGGT AGAGGAATAT CAGGAAGCAT TGGAGTAGTG 
GCTTCTATCA ACGAACAGGA AGGTATCGCT ACAGTCAGAT TTCCACCCAT AGACTGTCGA 
AAGACTTCTC AAGCGTCAGA CACATTGACT ATTCCATTGT CTAGACTTTG TGTTCCAAGG 
TCAGAGGCAC TGCCCCTCCA CAAACTGTCC ATTACTGAAA AGGTGGTACA GGCAGTTCAG 
TCCATGCTGC TTCCACAGGA AGGCAGTCTC TCAATTCACA CCTCGCTTCC TGCAACAGGA 
GATGGCTCTG CACCTGTGAT GGCAGTCGTC CGGCTCCTGG CTGAAATAAG AACAAGAGCA 
TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC AGCTTATTTT GTGAAGAATT CATACAGCAG 
TGTCCAGCAG CTGTTGAAGT TCTTAATCTG GTAGCCCAGG AATGCAGTGC TGGAGAGCGA 
CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG CTGAGGATGC TCTATCGGGA CTGCGCCCGG 
CCCCCGCCCC CTCCCCTGCA GGCTGACCGG AGACAGCCCA AAGAGATAAC TTGGAGCCCC 
TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC ATGTTCTCAT CTCACCTGAC CTCGGTCACA 
TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT CTGCCCCGGG GCACCTTTAT CTACGCCACC 
TCACCTCTGC CAGTTCAGGC CCCATCTTTT TACTGGGAAA TTGAAATTGT TTCCTATGGA 
GATACTGATG ACGACACTGG ACCAATAGTT TCCTTTGGCT TTGCTACAGA AGCCGAGAAA 
CGAGATGGAG CTTGGACCAA CCCAGTGGGC ACTTGCCTTT TCCACAACAA TGGCCGAGCC 
GTGCACTACA ACGGCTCCAG TCTGCTCCAG TGGAAGAGCG TTCGCTTAGA CGTGACTCTC 
TCTCCTGGTG ACGTGGCAGG AATTGGCTGG GAGAGAACCG AGGGTACTCC ACCTCCACCG 
GGGCAGCCAG CCAAGGGCCG GGTGTACTTC ACATATTGCG GGCAGCGCCT TTCACCATAC 
CTTGAGGATG TCTCTGGTGG GATGTGGCCT GTGGTTCACA TTCAGAAAAA GAACACCAAA 
ACTCGAGCCA ACTTTGGCTC CCGCCCATTT GCCTATGCCG AGGGGCAGGC CCATCGCAAC 
GCAGCTGACC TATGCACTGA CCTGGCAGAG GAGATTAGCG CAAACTTCGA GGCCCTGCCC 
TTTGCCATGG CATCTGACAG TGACAACGAT GCCGGCACCA GTATTGCATC AGACCCAGGC 
ACTCATGGGC CACCGTGCCG GATTGCAGCC GTGGCCACCG CTCAGCAACA ATACGATAGT 
GACACCTCCT GTCATTATAA AGTTGAGCTC AGCTATGAGA ATTTCATCAC CTCTGGCCCA 
GACCCTCACC CGCCTCCCAT TGCAGACGAT GAGAGTGATG ATGATGACGA TGATGACATT 
CCACAGGAGG ACCACTATGC CCTGCTGGTG AAAGCTTGGG AGACCAAGGT TTTTCCCACC 
ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG AGGAAATCCG GCCTGGACCA GATCAAGGGT 
GCTTTACAAC TAGGCATGGT GGATATTGCC CGGCAGACGG TGGAATTTCT ATATGAGGAG 
AATGGCGGCA TCCCAAGAGA CCTGTATCTT CCCACCATTG AGGATATTAA AGATGAAGCA 
AACAAGTTCA CAATTGACAA AGTTCGAAAA GGTCTCACAG TTGTAACTCG CTCTCCAGAC 
AGTAATAATG TAGCCAGCAG TACCGTTGGA ACTGCTTTGC CAAAATTTGC CATCCGTGGG 
ATGCTGAAAA CCTTTGGACT TCATGGAGTC GTCTTAGATG TCGATTCAGT GAACGAGCTG 
GTGCAGGTGG AAACATATCT CCGGAGTGAG GGGGTGCTGG TGCGGTATTG GTACCCCATT 
GACATGTTGG AAAGGCCCCC TGCAGGCTAC CGAAGGACTG CCACCAATGG GCTGGTCACA 
CTGGACAACA CCAACCTTCA AATTCACAGG GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC 
AGGCTCTACT GCCGCATGGC CCTACTCAAC ATCTTCGCCC CCAAGTTCCC TCACTTGTTT 
ACCCGCCTCT TCCACATCCC TGCCATCCGA GACATCACTC TGGAGCACCT GCAACTGCTG 
TCCAATCAGC TCCTCGCCCC TCCCCTCCCA GATGGCACCA TCAGCTCCAG CTCCATCCTC 
CTGGCGCAGT CCCTGCAGCA CTGCATACAC TCCCAGAACT GTTCCGCCAC GGACCTCTTT 
TACCAGGGCA GCTCCCAGAC AGTGAGAGAG TGGCTGAGTG TGGCCATCAC CCGCACCCTG 
CACCAGGGCG AGGAGAGCCT TCTGGAGCTC ACGAGGCAGA TCTGCTCCTT CCTGCAGACA 
GCACCAGAGC AGTTCCCCTC CGAAGAATTC CCGATATCCG AATCCAAAGT CAATATGGAT 
GTTAATTTTC CCGGGGCGGC TTTTGTTGTG GTGTCTTGTA AAGAAAGTCA ATCTGGATTC 
CGCAAAGACT CCTCTTTGTA CAAGGCACCC TGGGCACGCG TCCTCGTGTA CGGGCTTGGC 
CACAAAGTGA AGCGAACCGG CCAGCTGAAC CTCATCGAGG CTGCCTGTTA CCCACGGGAT 
GCGTCCCCGG CCAACACTGG GCTTGCACCT CCGCCCACAG CTGACCAGTA CCCTTCTGTG 
GTGCTCTCCA CAGACAGGGT GCACATCAAG CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG 
CTGGTATTGC ATTCTCTGCC CCTGGAGTTC CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG 
CTGTCATGGA AGTCGGAGGA TGGCGAAGGG AAGTCTGAAG ATGAGCCTGA CACTATTCCC 
ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG CTAGGAAATT TCCTGTGGAC CACAGACATG 
GCAGCCTGCG TGAAGGAACT TGTCTTCCAC CTCCTGGCAG AGCTCCTGCG CACGGTGCAC 
GCACTAGAGC AGAGGAAGCA CCCCGCCGGC CTGTCCTCCT CCATTGCCCT GCAGCTCAAC 
CCCTGTCTGG CCATGCTGAT GGCCCTGCAG TCAGAGCTGC ACAAGCTGTA TGACGAGGAG 
ACCCAGAGCT GGGTCTCAGG CAGCACCTGT GGGGGCTCTG GGGCAGTGGC AACCGGCGAC 
CAGGGCAGGT TCTCCACGTA CTTTCATGCA CTCATGGAAG GGTGCCTGGC AGTTGCCGAA 
GTGACCCTGC CTACCAACAT GAGTGTCACA GCCAGTGGAG TGACCTCAGC AACCACCCCA 
AACCTCAGTG ACTCGTCGTC CTCCTCCTCT TCTTCTCCAG GACAGACACC GCAGAGTCCC 
AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG 
AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC GACTCCGCCG TGCTCAGCAT CGGGGGCAGC 
AAGCCTGAGG ACATGCTCTG GTTCCACCGC GCGCTCACCT TGCTCATCAT TCTGCGGCAC 
CTCACCAGGA AGGACCCCCA GGGCCTGGGT GTGACCAGCG ATGCCGTTGC AGATGCCTGC 
CAGGCCCTAG TGGGCCCCAC TGCCCATAGC CGCCTGCTGG TGATCTCTGG CATCCCTACC 
CACCTGGACG AGGGCATCGT CAGAGGTGCT ATCCGCAAGG CCTGCAATGC CCACGGCGGG 
GTCTTCAAAG ATGAGATCTA CATTCCACTG CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC 
AAGGCCGAAG GTGGCGATGG GAAGGCCGAG CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA 
GACAGCCTGG AGGGGAGCAC GTCCAGCAGC CTGGCCCCGG CCATGAGCAT CAGTGCCTCT 
GCCTCCACCA GCCAAGCCTC TGTCTGCAGC TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC 
CTCTCGGTGG ACCCACTGCC GTCCGGCCTG GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG 
CCCCACGTGG TGTCCAGCCA GGAGAGCCTG GATATTTCCC TGTGCAGCAC TGGCAGCCTG 
GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA GACAATGCGG AGACAGCCTC GGTGTCAGAC 
ATGGGCTCCA TGTACACAGT CACTTCCCTG GACAACCAGT CCCTTGCGGC ACGCCCCATC 
AAAGGCTTTG CCGTCGTGGA GATAAGATCC CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT 
TTATTTAACA ATAATGATTT AATTGGCTTA TCAAGTTTGG ATGGTGAAGA TGAATTGATG 
GAAATGTCCA CAGAAGAGAT TCTGACTGTA TCTGTAGTGA ACCAGAGTTT GTTTGATACT 
CAAGGGAGCC CGGGGTTAGA AGATTATTTC AATGATAAGT CAATTAAAGG TGAGAAGCTG 
GTGCCTGGGG CCAGAGAGGT CCTGACAGAG ATATTTAAGA GCTGTGCCCA CTCAGAGCAG 
ATGCTGAGCC TGACACCAGC AAAGCCCATC AGGGTCTCTG ACATATATCT CAGCAAGGAG 
CAGATCAACT CCCAGACTCC AGGCAACCTC CTCCACATCT TCTTCACCAA TGTCCGGCCT 
CCCAAGAAGG TGCTAGAAGA CCAGCTCACC CAGATCCTGA GGAAATATGG CGTGCCGAAG 
CCCAAGTTTG ACAAGAGCAA GTACAGCAAA GCTGGGAAGG AGCAGCACCC CGTGAAGGTG 
GTGAGCACCA AGCGGCCCAT CACCAAGCCG CCTGCCAAGG ACAAAGCCGT GCTCAACAGT 
GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA CCAACTGTGA AGCCCAAGTC ACCAGAAAAG 
AGCAAACCAG ACGAAAAGGA TCCAGAAAAG TCGCCCACAA AAAAACAAGA AGTCCCCGAG 
GAGAAGTACC TGACCTTAGA AGGGTTTCAC AAGTTTGTCA TCGACCGAGC CAAGCAGGAC 
ATCCGCAGCG TGTGGAGGGC AATTCTGTCT TGTGGCTACG ATCTTCATTT TGAGAGGTGT 
GCCTGCATTG ATGTCCGACA TGCGCAGAAG GCCTCAAGGA AGTGGACCCT GGAGATGGAT 
GTAGCTCTCG TGCAGTACAT CAATCGGCTG TGCCGCCACC TTGCCATCAC ACCTGCAAGG 
CTCCACCCCC ACGAGGTTTA CCTGGACCCT GCCGATGCCA CTGACCCCAG GGTGGCCTGT 
CTCTTGAATG TGCCCATTGA GAGCCTGCGC CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC 
ACCACGCTGG AGACCTTCTT CCTGCCCCTG GTGGAGCTGC GCCAGACACC CATGTACACC 
CACAGCATCG CGGCCCTTCT GAAGGAGGCC AAAGGGCTGA TCTTCTATGA CACAAAGGTG 
ACCGTGATGA ACCGAGTGCT GAATGCCACT GTGCAGAGGA CAGCTGACCA CGCGGCACCC 
GAGATCACCT TGGACCCATT GGAAATTGTG GGAGGGGAAA TCAGAGCTTC TGAAAACTCC 
TACTTCTGCC AGGCTGCCAG GCAACTGGCC TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG 
CTGGCGAGTG GCGGCGACCC CACCTACGCC TTCAACATCC GCTTCACCGG GGAGGAGGTC 
CACGGCACCA GTGGCTCCTT CCGCCACTTC CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC 
TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC AGCTCTGCTG TCAACAAGAA CAAGGGCAAG 
TACATCCTGA CGCCCAGCCC CATCACCTAC GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC 
CAGTTGCTGG GGATCGCCAT CCGGGCAGAT GTCCCCCTTC CCCTGGACCT CCTGCCCTCC 
TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG GACCCTGACC AGGACCTGCA GGAAGCAGAC 
ATCCTTACCT ATAATTACGT CAAGAAGTTT GAGAGCATCA ACGACGAGAG CGAGCTGGAA 
GCCCTGTGCG CCGAGATTGC CTCCCAGCAC CTGGCCACCG AGAGTCCTGA CGGCCCCAGC 
AAGCCCTGCT GCAGGTTCAC CTACCTGACC ATGACGGGCG AGGAGGTGGA GCTGTGCAGC 
CGCGGCCGGC ACATCCCTGT GGCGTGGGAG AACAAGGACA TCTACGCTGC AGCCATCCGC 
AGCCTGCGCT TGCGGGAGCT GCAGAACGTG GAATGCGTGA CAGCCGTGCG CGCAGGCCTG 
GGCTCTATCA TCCCCCTGCA GCTGCTCACC ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC 
ACCTGTGGCC TCCCCTACAT CAACCTCGAG TTCCTGAAGG CCCATACCAT GTACCAGGTG 
GGGCTAATGG AGACGGACCA ACACATCGAG TTCTTCTGGG GGGCCCTGGA GACGTTCACC 
CAGGAGGAGC TGTGCAGGTT CATCAAGTTC GCCTGCAACC AGGAGCGCAT CCCCTTCACC 
TGCCCCTGTA AAGATGGGGG CCCCGACACA GCCCACGTAC CCCCCTACCC CATGAAGATC 
GCCCCCCCAG ATGGCACAGC AGGTTCCCCA GACTCCCGCT ACATCCGGGT GGAGACCTGC 
ATGTTCATGA TCAAGCTCCC CCAGTACTCC TCTTTGGAAA TCATGCTGGA GAAACTGCGC 
TGTGCCATTC ACTACAGGGA AGACCCCCTA AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219021.1
CDS	205..13023
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_013363567.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain containing E3 ubiquitin protein ligase 4 (Hectd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013363567.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT TGGTGATATA TGCGGGGCCT AACACAAACA GTAGGAAGGT TGCTGAATAT 
GGAGGCAATA CTCTGGGATA TGGCAGCCGT AGTGTCTTAG GAACTGGTTG GCCGAAGGAC 
TTGGTGAAGG TGGAAGGAGA TACAGTCACC TTCTCCTTTG AAATGAGAAG TGGCCGTGAA 
CACAACACTC CTGATAAAGC TATGTGGGGC TTTGCGTGCA CAGTTCGCGC TCAGGAGTCC 
TCGGAGGACG TCTCAGGAGG CCTGCCTTTT CTGGTGGACC TGGCTTTAGG TCTGTCTGTG 
TTAGCTTGTT CCATGTTAAG AATCCTGTAC AATGGACCAG AAATTACCAA AGAAGAGGAA 
GCCTGTCAGG AGCTTTTGCG GTCCAAACTT TTACAAAGGT GCCAGTGGCA GGTGGAGGCC 
AATGGCGTCA TCTCGCCTGC CCTCACCCCA AGTCCCTCTC CACTGCCTCT GACCATAGAG 
GAAGACCGAG AGTTCACCTA CCCCTCGGAT GTCCTCGTGC CTCCTGTTGG GAACTACTTT 
GACCTGCCCC GGATCCGGCT GCCTCCAGGA ATCATGATAA AGCTCAGGGA AATTTCTGGG 
CGAGCTAGAC CTCAATTTAG ACCAAGTATA AAGGAAGTGA TTCAGCCAGA TGTGATGGAG 
GAAATGGTGG TATCATGTGT TATTAAGCAC TTGAACTTGG TCGATGCACT GCAATCCCTA 
ATAAATTTCC AATATCAAGA AGAACATGCT GAGGAATATG ACTTACTATG TAAAATTATG 
GGAGAGACCT TTAAGAAACT CAATGCCATG GAGAGACAGC TGCAGAGTGT TGCAGAACTG 
GAACAGAAGT GGCAAAGTGA GGTTGATGAT GCCATGCAGG GGAAATTGGA GAACAACATG 
CCTTTCTTTT ATGATTACCA TTTTAATGAG AACAAAATGA AAGAACTAGA ACTTTTGTGT 
TCAATGAAGG AAGTCTCCTT TGATGGAAAT GATCTTGAAA ACATGGTCCT ATCACTGAGG 
GAGAAGTTCC TACAAGAAGT GAATTCTCTT ATTCAGAAGC CCTCTCATCC ACTGGCTAAA 
ACGAAGACAT TAGTGAAGAG TCTAATGAAC CGTGCTGAGC TGCTGCTGCA TGTCACCATT 
GCGGCCCAAT CTGGCCTCAC CAGGAGCATC TCGGGAACCC CTGCAGAGAC ACCGGCTTGT 
AAATCAGCTT CTGAAACAAA GGTGATATCT CATGCCGTCC GGCAGCCTGT TTTTCTCCGT 
AGTATGTCGG CTCCTTCTGA TCTAGAAATG ATTGGTAATG AAGATTTGGA ATTTACTAGA 
GCAAGTCAGA GGCGTCGACA TGTGACCAGC CACCGGAGCA GCTCCTTTAC ACTCCTGCAA 
TCGTTGGCCA TTGAGGACAG CAGGGACAAG CCCACCTATA GTGTCCTGCT GGGGCAGCTG 
TTTGCTTTCA TTGGCACCAA TCCCGACCAA GCTGTGTCCA GCAGCAGCTT CCTTCTTGCT 
GCACAGACGA GGTGGCGGAG GGGAAACACA CGCAAGCAGG CCCTGGTGCA CATGCGAGAG 
CTGCTCACAG CTGCCGTGCG CGTTGGGGGA GTGACACATC TTGTGGGGCC AGTGACTATG 
GTCCTCCAGG GAGGACCCAG GATTGAAGAG CTCACCTGTG GTGGGATGGT TGAGCAGGTT 
CAGGAAGCCT TTGGCGAAAC CATGACTTCG GTTGTGTCTC TGTGTGCCCG TTACCCTATC 
GCTTGTGCAA ATAGCATTGG ACTCTTGTGT ACCATACCTT ATACCAGGAG TGAGGAGAAG 
TGCCTGGTGC GCAGTGGCCT TGTCCAGCTC ATGGATCGAC TGTGCAGCTT GAGCAGTCAG 
ACCGAGTCCA GCTCCAGTGA GAAACAAACC AAGAAGCAGA AAGTGGCCAC CATGGCCTGG 
GCTGCCTTCC AGGTGCTGGC CAACCGCTGT GTCGAGTGGG AAAAAGAGGA AGGTGGCTCC 
ACAGAGGCTG TGCACTCGGG GCTGGCTCGG CAGGTGTCCA GCCTTCTCAC GAATCATCTT 
GCCCGAGCCA CTGAGTGCTG TGGCAACCAG GCTGCAGGAA ATGATGCTCT GCAGGATGTC 
CTGAGTCTGC TCAACGACCT CTCGAGGAGC CACATAGGGA AAGCCATCCT GAGCCAGCCA 
GCTTGTGTGT CCAAGCTCCT CTCCCTGCTG CTTGACCAGC GCCCATCTCC AAAATTGGTC 
CTTATCATTC TTCAACTGTG CCGGGCGGCA CTGCCATTGA TGAGCGTAGA GGACTGTGGA 
AATGTGGAAC TCCCGCCATG GAGCTATTCT GTCCCCTCCC TAAACAGTGA GCAGGAGGAT 
CCCAGTGACC CAGCTTCCAA GATCGCCTCC TTGCTCTTAG CAAAGCTGGC AGATTACGTG 
GTCCCAGGAT GTCAGACAGT TCTTTCTCCA ACTGCTTCTG AACCGGATAC CACATTAACA 
AAAACCAGTC CCAAGAATTC TTTAAAAGGA GATAAAGATC CTGGAGAAGA GAGTGAAGCA 
GTGGACGGCA AGCTCTCCAT ATTTATCCAC AAACGGGAAG ACCAGTCCTC CCACGAAGTT 
CTCCAACCAT TACTAAGTAG CTCAGAAGGG CGGCCCTTCC GACTTGGTAC TGGTGCCAAC 
ATGGAGAAAG TTGTGAAGAT GGATAGAGAC ATGACCAAGG GTGGCTGTTG CGAAGTGATC 
ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA GCCACCAAGT GGGCACAGTC AGGCCTCATC 
GTCAGCATTG GGCCACCAGT AGAGTCTGTC AATCCAGAGA CTGTGAGTGG ACTATCCACA 
GGTGACAAAA AGAAAACTGC CCAAACCTCC ATCTGCAGAG AGAGGAACTC AGAACTCGCC 
AGGACTGACC CTGTCCGACC CTTCATCAGT GGGCATGTGG CAAACAGCAT GGCTGCAGAA 
GTGATCGCTT TGCTTCATAG CTTGCTAATG GCACCTGAAT CTAACGCGGC TCAAATATGG 
ACCACAACAG CAGAAAAGGT TCTGTCTCGT GCATTGATGT ACATTCCACA ACTGGGGAAA 
CATGCAGAAA GCATTCTGGA AAATGGCAGC AGCAGTGGCA GAAAACTTGC CAAACTTCAG 
AGAATTGCTC GCCAGGCTGT CGCTGCACTG TGTGCACTTG GAGGCTTCAA AGAGACAATC 
AAGATAGGAT CTGAGGTTCA GGTTTTAGGT AGAGGAATAT CAGGAAGCAT TGGAGTAGTG 
GCTTCTATCA ACGAACAGGA AGGTATCGCT ACAGTCAGAT TTCCACCCAT AGACTGTCGA 
AAGACTTCTC AAGCGTCAGA CACATTGACT ATTCCATTGT CTAGACTTTG TGTTCCAAGG 
TCAGAGGCAC TGCCCCTCCA CAAACTGTCC ATTACTGAAA AGGTGGTACA GGCAGTTCAG 
TCCATGCTGC TTCCACAGGA AGGCAGTCTC TCAATTCACA CCTCGCTTCC TGCAACAGGA 
GATGGCTCTG CACCTGTGAT GGCAGTCGTC CGGCTCCTGG CTGAAATAAG AACAAGAGCA 
TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC AGCTTATTTT GTGAAGAATT CATACAGCAG 
TGTCCAGCAG CTGTTGAAGT TCTTAATCTG GTAGCCCAGG AATGCAGTGC TGGAGAGCGA 
CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG CTGAGGATGC TCTATCGGGA CTGCGCCCGG 
CCCCCGCCCC CTCCCCTGCA GGCTGACCGG AGACAGCCCA AAGAGATAAC TTGGAGCCCC 
TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC ATGTTCTCAT CTCACCTGAC CTCGGTCACA 
TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT CTGCCCCGGG GCACCTTTAT CTACGCCACC 
TCACCTCTGC CAGTTCAGGC CCCATCTTTT TACTGGGAAA TTGAAATTGT TTCCTATGGA 
GATACTGATG ACGACACTGG ACCAATAGTT TCCTTTGGCT TTGCTACAGA AGCCGAGAAA 
CGAGATGGAG CTTGGACCAA CCCAGTGGGC ACTTGCCTTT TCCACAACAA TGGCCGAGCC 
GTGCACTACA ACGGCTCCAG TCTGCTCCAG TGGAAGAGCG TTCGCTTAGA CGTGACTCTC 
TCTCCTGGTG ACGTGGCAGG AATTGGCTGG GAGAGAACCG AGGGTACTCC ACCTCCACCG 
GGGCAGCCAG CCAAGGGCCG GGTGTACTTC ACATATTGCG GGCAGCGCCT TTCACCATAC 
CTTGAGGATG TCTCTGGTGG GATGTGGCCT GTGGTTCACA TTCAGAAAAA GAACACCAAA 
ACTCGAGCCA ACTTTGGCTC CCGCCCATTT GCCTATGCCG AGGGGCAGGC CCATCGCAAC 
GCAGCTGACC TATGCACTGA CCTGGCAGAG GAGATTAGCG CAAACTTCGA GGCCCTGCCC 
TTTGCCATGG CATCTGACAG TGACAACGAT GCCGGCACCA GTATTGCATC AGACCCAGGC 
ACTCATGGGC CACCGTGCCG GATTGCAGCC GTGGCCACCG CTCAGCAACA ATACGATAGT 
GACACCTCCT GTCATTATAA AGTTGAGCTC AGCTATGAGA ATTTCATCAC CTCTGGCCCA 
GACCCTCACC CGCCTCCCAT TGCAGACGAT GAGAGTGATG ATGATGACGA TGATGACATT 
CCACAGGAGG ACCACTATGC CCTGCTGGTG AAAGCTTGGG AGACCAAGGT TTTTCCCACC 
ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG AGGAAATCCG GCCTGGACCA GATCAAGGGT 
GCTTTACAAC TAGGCATGGT GGATATTGCC CGGCAGACGG TGGAATTTCT ATATGAGGAG 
AATGGCGGCA TCCCAAGAGA CCTGTATCTT CCCACCATTG AGGATATTAA AGATGAAGCA 
AACAAGTTCA CAATTGACAA AGTTCGAAAA GGTCTCACAG TTGTAACTCG CTCTCCAGAC 
AGTAATAATG TAGCCAGCAG TACCGTTGGA ACTGCTTTGC CAAAATTTGC CATCCGTGGG 
ATGCTGAAAA CCTTTGGACT TCATGGAGTC GTCTTAGATG TCGATTCAGT GAACGAGCTG 
GTGCAGGTGG AAACATATCT CCGGAGTGAG GGGGTGCTGG TGCGGTATTG GTACCCCATT 
GACATGTTGG AAAGGCCCCC TGCAGGCTAC CGAAGGACTG CCACCAATGG GCTGGTCACA 
CTGGACAACA CCAACCTTCA AATTCACAGG GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC 
AGGCTCTACT GCCGCATGGC CCTACTCAAC ATCTTCGCCC CCAAGTTCCC TCACTTGTTT 
ACCCGCCTCT TCCACATCCC TGCCATCCGA GACATCACTC TGGAGCACCT GCAACTGCTG 
TCCAATCAGC TCCTCGCCCC TCCCCTCCCA GATGGCACCA TCAGCTCCAG CTCCATCCTC 
CTGGCGCAGT CCCTGCAGCA CTGCATACAC TCCCAGAACT GTTCCGCCAC GGACCTCTTT 
TACCAGGGCA GCTCCCAGAC AGTGAGAGAG TGGCTGAGTG TGGCCATCAC CCGCACCCTG 
CACCAGGGCG AGGAGAGCCT TCTGGAGCTC ACGAGGCAGA TCTGCTCCTT CCTGCAGACA 
GCACCAGAGC AGTTCCCCTC CGAAGAATTC CCGATATCCG AATCCAAAGT CAATATGGAT 
GTTAATTTTC CCGGGGCGGC TTTTGTTGTG GTGTCTTGTA AAGAAAGTCA ATCTGGATTC 
CGCAAAGACT CCTCTTTGTA CAAGGCACCC TGGGCACGCG TCCTCGTGTA CGGGCTTGGC 
CACAAAGTGA AGCGAACCGG CCAGCTGAAC CTCATCGAGG CTGCCTGTTA CCCACGGGAT 
GCGTCCCCGG CCAACACTGG GCTTGCACCT CCGCCCACAG CTGACCAGTA CCCTTCTGTG 
GTGCTCTCCA CAGACAGGGT GCACATCAAG CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG 
CTGGTATTGC ATTCTCTGCC CCTGGAGTTC CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG 
CTGTCATGGA AGTCGGAGGA TGGCGAAGGG AAGTCTGAAG ATGAGCCTGA CACTATTCCC 
ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG CTAGGAAATT TCCTGTGGAC CACAGACATG 
GCAGCCTGCG TGAAGGAACT TGTCTTCCAC CTCCTGGCAG AGCTCCTGCG CACGGTGCAC 
GCACTAGAGC AGAGGAAGCA CCCCGCCGGC CTGTCCTCCT CCATTGCCCT GCAGCTCAAC 
CCCTGTCTGG CCATGCTGAT GGCCCTGCAG TCAGAGCTGC ACAAGCTGTA TGACGAGGAG 
ACCCAGAGCT GGGTCTCAGG CAGCACCTGT GGGGGCTCTG GGGCAGTGGC AACCGGCGAC 
CAGGGCAGGT TCTCCACGTA CTTTCATGCA CTCATGGAAG GGTGCCTGGC AGTTGCCGAA 
GTGACCCTGC CTACCAACAT GAGTGTCACA GCCAGTGGAG TGACCTCAGC AACCACCCCA 
AACCTCAGTG ACTCGTCGTC CTCCTCCTCT TCTTCTCCAG GACAGACACC GCAGAGTCCC 
AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG 
AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC GACTCCGCCG TGCTCAGCAT CGGGGGCAGC 
AAGCCTGAGG ACATGCTCTG GTTCCACCGC GCGCTCACCT TGCTCATCAT TCTGCGGCAC 
CTCACCAGGA AGGACCCCCA GGGCCTGGGT GTGACCAGCG ATGCCGTTGC AGATGCCTGC 
CAGGCCCTAG TGGGCCCCAC TGCCCATAGC CGCCTGCTGG TGATCTCTGG CATCCCTACC 
CACCTGGACG AGGGCATCGT CAGAGGTGCT ATCCGCAAGG CCTGCAATGC CCACGGCGGG 
GTCTTCAAAG ATGAGATCTA CATTCCACTG CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC 
AAGGCCGAAG GTGGCGATGG GAAGGCCGAG CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA 
GACAGCCTGG AGGGGAGCAC GTCCAGCAGC CTGGCCCCGG CCATGAGCAT CAGTGCCTCT 
GCCTCCACCA GCCAAGCCTC TGTCTGCAGC TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC 
CTCTCGGTGG ACCCACTGCC GTCCGGCCTG GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG 
CCCCACGTGG TGTCCAGCCA GGAGAGCCTG GATATTTCCC TGTGCAGCAC TGGCAGCCTG 
GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA GACAATGCGG AGACAGCCTC GGTGTCAGAC 
ATGGGCTCCA TGTACACAGT CACTTCCCTG GACAACCAGT CCCTTGCGGC ACGCCCCATC 
AAAGGCTTTG CCGTCGTGGA GATAAGATCC CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT 
TTATTTAACA ATAATGATTT AATTGGCTTA TCAAGTTTGG ATGGTGAAGA TGAATTGATG 
GAAATGTCCA CAGAAGAGAT TCTGACTGTA TCTGTAGTGA ACCAGAGTTT GTTTGATACT 
CAAGGGAGCC CGGGGTTAGA AGATTATTTC AATGATAAGT CAATTAAAGG TGAGAAGCTG 
GTGCCTGGGG CCAGAGAGGT CCTGACAGAG ATATTTAAGA GCTGTGCCCA CTCAGAGCAG 
ATGCTGAGCC TGACACCAGC AAAGCCCATC AGGGTCTCTG ACATATATCT CAGCAAGGAG 
CAGATCAACT CCCAGACTCC AGGCAACCTC CTCCACATCT TCTTCACCAA TGTCCGGCCT 
CCCAAGAAGG TGCTAGAAGA CCAGCTCACC CAGATCCTGA GGAAATATGG CGTGCCGAAG 
CCCAAGTTTG ACAAGAGCAA GTACAGCAAA GCTGGGAAGG AGCAGCACCC CGTGAAGGTG 
GTGAGCACCA AGCGGCCCAT CACCAAGCCG CCTGCCAAGG ACAAAGCCGT GCTCAACAGT 
GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA CCAACTGTGA AGCCCAAGTC ACCAGAAAAG 
AGCAAACCAG ACGAAAAGGA TCCAGAAAAG TCGCCCACAA AAAAACAAGA AGTCCCCGAG 
GAGAAGTACC TGACCTTAGA AGGGTTTCAC AAGTTTGTCA TCGACCGAGC CAAGCAGGAC 
ATCCGCAGCG TGTGGAGGGC AATTCTGTCT TGTGGCTACG ATCTTCATTT TGAGAGGTGT 
GCCTGCATTG ATGTCCGACA TGCGCAGAAG GCCTCAAGGA AGTGGACCCT GGAGATGGAT 
GTAGCTCTCG TGCAGTACAT CAATCGGCTG TGCCGCCACC TTGCCATCAC ACCTGCAAGG 
CTCCACCCCC ACGAGGTTTA CCTGGACCCT GCCGATGCCA CTGACCCCAG GGTGGCCTGT 
CTCTTGAATG TGCCCATTGA GAGCCTGCGC CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC 
ACCACGCTGG AGACCTTCTT CCTGCCCCTG GTGGAGCTGC GCCAGACACC CATGTACACC 
CACAGCATCG CGGCCCTTCT GAAGGAGGCC AAAGGGCTGA TCTTCTATGA CACAAAGGTG 
ACCGTGATGA ACCGAGTGCT GAATGCCACT GTGCAGAGGA CAGCTGACCA CGCGGCACCC 
GAGATCACCT TGGACCCATT GGAAATTGTG GGAGGGGAAA TCAGAGCTTC TGAAAACTCC 
TACTTCTGCC AGGCTGCCAG GCAACTGGCC TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG 
CTGGCGAGTG GCGGCGACCC CACCTACGCC TTCAACATCC GCTTCACCGG GGAGGAGGTC 
CACGGCACCA GTGGCTCCTT CCGCCACTTC CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC 
TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC AGCTCTGCTG TCAACAAGAA CAAGGGCAAG 
TACATCCTGA CGCCCAGCCC CATCACCTAC GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC 
CAGTTGCTGG GGATCGCCAT CCGGGCAGAT GTCCCCCTTC CCCTGGACCT CCTGCCCTCC 
TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG GACCCTGACC AGGACCTGCA GGAAGCAGAC 
ATCCTTACCT ATAATTACGT CAAGAAGTTT GAGAGCATCA ACGACGAGAG CGAGCTGGAA 
GCCCTGTGCG CCGAGATTGC CTCCCAGCAC CTGGCCACCG AGAGTCCTGA CGGCCCCAGC 
AAGCCCTGCT GCAGGTTCAC CTACCTGACC ATGACGGGCG AGGAGGTGGA GCTGTGCAGC 
CGCGGCCGGC ACATCCCTGT GGCGTGGGAG AACAAGGACA TCTACGCTGC AGCCATCCGC 
AGCCTGCGCT TGCGGGAGCT GCAGAACGTG GAATGCGTGA CAGCCGTGCG CGCAGGCCTG 
GGCTCTATCA TCCCCCTGCA GCTGCTCACC ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC 
ACCTGTGGCC TCCCCTACAT CAACCTCGAG TTCCTGAAGG CCCATACCAT GTACCAGGTG 
GGGCTAATGG AGACGGACCA ACACATCGAG TTCTTCTGGG GGGCCCTGGA GACGTTCACC 
CAGGAGGAGC TGTGCAGGTT CATCAAGTTC GCCTGCAACC AGGAGCGCAT CCCCTTCACC 
TGCCCCTGTA AAGATGGGGG CCCCGACACA GCCCACGTAC CCCCCTACCC CATGAAGATC 
GCCCCCCCAG ATGGCACAGC AGGTTCCCCA GACTCCCGCT ACATCCGGGT GGAGACCTGC 
ATGTTCATGA TCAAGCTCCC CCAGTACTCC TCTTTGGAAA TCATGCTGGA GAAACTGCGC 
TGTGCCATTC ACTACAGGGA AGACCCCCTA AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_013363567.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_013219021.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X6, mRNA.

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CloneID	OIc67628
Clone ID Related Accession (Same CDS sequence)	XM_013363567.1 , XM_013363567.2
Accession Version	XM_013363567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_013363567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT TGGTGATATA TGCGGGGCCT AACACAAACA GTAGGAAGGT TGCTGAATAT 
GGAGGCAATA CTCTGGGATA TGGCAGCCGT AGTGTCTTAG GAACTGGTTG GCCGAAGGAC 
TTGGTGAAGG TGGAAGGAGA TACAGTCACC TTCTCCTTTG AAATGAGAAG TGGCCGTGAA 
CACAACACTC CTGATAAAGC TATGTGGGGC TTTGCGTGCA CAGTTCGCGC TCAGGAGTCC 
TCGGAGGACG TCTCAGGAGG CCTGCCTTTT CTGGTGGACC TGGCTTTAGG TCTGTCTGTG 
TTAGCTTGTT CCATGTTAAG AATCCTGTAC AATGGACCAG AAATTACCAA AGAAGAGGAA 
GCCTGTCAGG AGCTTTTGCG GTCCAAACTT TTACAAAGGT GCCAGTGGCA GGTGGAGGCC 
AATGGCGTCA TCTCGCCTGC CCTCACCCCA AGTCCCTCTC CACTGCCTCT GACCATAGAG 
GAAGACCGAG AGTTCACCTA CCCCTCGGAT GTCCTCGTGC CTCCTGTTGG GAACTACTTT 
GACCTGCCCC GGATCCGGCT GCCTCCAGGA ATCATGATAA AGCTCAGGGA AATTTCTGGG 
CGAGCTAGAC CTCAATTTAG ACCAAGTATA AAGGAAGTGA TTCAGCCAGA TGTGATGGAG 
GAAATGGTGG TATCATGTGT TATTAAGCAC TTGAACTTGG TCGATGCACT GCAATCCCTA 
ATAAATTTCC AATATCAAGA AGAACATGCT GAGGAATATG ACTTACTATG TAAAATTATG 
GGAGAGACCT TTAAGAAACT CAATGCCATG GAGAGACAGC TGCAGAGTGT TGCAGAACTG 
GAACAGAAGT GGCAAAGTGA GGTTGATGAT GCCATGCAGG GGAAATTGGA GAACAACATG 
CCTTTCTTTT ATGATTACCA TTTTAATGAG AACAAAATGA AAGAACTAGA ACTTTTGTGT 
TCAATGAAGG AAGTCTCCTT TGATGGAAAT GATCTTGAAA ACATGGTCCT ATCACTGAGG 
GAGAAGTTCC TACAAGAAGT GAATTCTCTT ATTCAGAAGC CCTCTCATCC ACTGGCTAAA 
ACGAAGACAT TAGTGAAGAG TCTAATGAAC CGTGCTGAGC TGCTGCTGCA TGTCACCATT 
GCGGCCCAAT CTGGCCTCAC CAGGAGCATC TCGGGAACCC CTGCAGAGAC ACCGGCTTGT 
AAATCAGCTT CTGAAACAAA GGTGATATCT CATGCCGTCC GGCAGCCTGT TTTTCTCCGT 
AGTATGTCGG CTCCTTCTGA TCTAGAAATG ATTGGTAATG AAGATTTGGA ATTTACTAGA 
GCAAGTCAGA GGCGTCGACA TGTGACCAGC CACCGGAGCA GCTCCTTTAC ACTCCTGCAA 
TCGTTGGCCA TTGAGGACAG CAGGGACAAG CCCACCTATA GTGTCCTGCT GGGGCAGCTG 
TTTGCTTTCA TTGGCACCAA TCCCGACCAA GCTGTGTCCA GCAGCAGCTT CCTTCTTGCT 
GCACAGACGA GGTGGCGGAG GGGAAACACA CGCAAGCAGG CCCTGGTGCA CATGCGAGAG 
CTGCTCACAG CTGCCGTGCG CGTTGGGGGA GTGACACATC TTGTGGGGCC AGTGACTATG 
GTCCTCCAGG GAGGACCCAG GATTGAAGAG CTCACCTGTG GTGGGATGGT TGAGCAGGTT 
CAGGAAGCCT TTGGCGAAAC CATGACTTCG GTTGTGTCTC TGTGTGCCCG TTACCCTATC 
GCTTGTGCAA ATAGCATTGG ACTCTTGTGT ACCATACCTT ATACCAGGAG TGAGGAGAAG 
TGCCTGGTGC GCAGTGGCCT TGTCCAGCTC ATGGATCGAC TGTGCAGCTT GAGCAGTCAG 
ACCGAGTCCA GCTCCAGTGA GAAACAAACC AAGAAGCAGA AAGTGGCCAC CATGGCCTGG 
GCTGCCTTCC AGGTGCTGGC CAACCGCTGT GTCGAGTGGG AAAAAGAGGA AGGTGGCTCC 
ACAGAGGCTG TGCACTCGGG GCTGGCTCGG CAGGTGTCCA GCCTTCTCAC GAATCATCTT 
GCCCGAGCCA CTGAGTGCTG TGGCAACCAG GCTGCAGGAA ATGATGCTCT GCAGGATGTC 
CTGAGTCTGC TCAACGACCT CTCGAGGAGC CACATAGGGA AAGCCATCCT GAGCCAGCCA 
GCTTGTGTGT CCAAGCTCCT CTCCCTGCTG CTTGACCAGC GCCCATCTCC AAAATTGGTC 
CTTATCATTC TTCAACTGTG CCGGGCGGCA CTGCCATTGA TGAGCGTAGA GGACTGTGGA 
AATGTGGAAC TCCCGCCATG GAGCTATTCT GTCCCCTCCC TAAACAGTGA GCAGGAGGAT 
CCCAGTGACC CAGCTTCCAA GATCGCCTCC TTGCTCTTAG CAAAGCTGGC AGATTACGTG 
GTCCCAGGAT GTCAGACAGT TCTTTCTCCA ACTGCTTCTG AACCGGATAC CACATTAACA 
AAAACCAGTC CCAAGAATTC TTTAAAAGGA GATAAAGATC CTGGAGAAGA GAGTGAAGCA 
GTGGACGGCA AGCTCTCCAT ATTTATCCAC AAACGGGAAG ACCAGTCCTC CCACGAAGTT 
CTCCAACCAT TACTAAGTAG CTCAGAAGGG CGGCCCTTCC GACTTGGTAC TGGTGCCAAC 
ATGGAGAAAG TTGTGAAGAT GGATAGAGAC ATGACCAAGG GTGGCTGTTG CGAAGTGATC 
ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA GCCACCAAGT GGGCACAGTC AGGCCTCATC 
GTCAGCATTG GGCCACCAGT AGAGTCTGTC AATCCAGAGA CTGTGAGTGG ACTATCCACA 
GGTGACAAAA AGAAAACTGC CCAAACCTCC ATCTGCAGAG AGAGGAACTC AGAACTCGCC 
AGGACTGACC CTGTCCGACC CTTCATCAGT GGGCATGTGG CAAACAGCAT GGCTGCAGAA 
GTGATCGCTT TGCTTCATAG CTTGCTAATG GCACCTGAAT CTAACGCGGC TCAAATATGG 
ACCACAACAG CAGAAAAGGT TCTGTCTCGT GCATTGATGT ACATTCCACA ACTGGGGAAA 
CATGCAGAAA GCATTCTGGA AAATGGCAGC AGCAGTGGCA GAAAACTTGC CAAACTTCAG 
AGAATTGCTC GCCAGGCTGT CGCTGCACTG TGTGCACTTG GAGGCTTCAA AGAGACAATC 
AAGATAGGAT CTGAGGTTCA GGTTTTAGGT AGAGGAATAT CAGGAAGCAT TGGAGTAGTG 
GCTTCTATCA ACGAACAGGA AGGTATCGCT ACAGTCAGAT TTCCACCCAT AGACTGTCGA 
AAGACTTCTC AAGCGTCAGA CACATTGACT ATTCCATTGT CTAGACTTTG TGTTCCAAGG 
TCAGAGGCAC TGCCCCTCCA CAAACTGTCC ATTACTGAAA AGGTGGTACA GGCAGTTCAG 
TCCATGCTGC TTCCACAGGA AGGCAGTCTC TCAATTCACA CCTCGCTTCC TGCAACAGGA 
GATGGCTCTG CACCTGTGAT GGCAGTCGTC CGGCTCCTGG CTGAAATAAG AACAAGAGCA 
TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC AGCTTATTTT GTGAAGAATT CATACAGCAG 
TGTCCAGCAG CTGTTGAAGT TCTTAATCTG GTAGCCCAGG AATGCAGTGC TGGAGAGCGA 
CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG CTGAGGATGC TCTATCGGGA CTGCGCCCGG 
CCCCCGCCCC CTCCCCTGCA GGCTGACCGG AGACAGCCCA AAGAGATAAC TTGGAGCCCC 
TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC ATGTTCTCAT CTCACCTGAC CTCGGTCACA 
TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT CTGCCCCGGG GCACCTTTAT CTACGCCACC 
TCACCTCTGC CAGTTCAGGC CCCATCTTTT TACTGGGAAA TTGAAATTGT TTCCTATGGA 
GATACTGATG ACGACACTGG ACCAATAGTT TCCTTTGGCT TTGCTACAGA AGCCGAGAAA 
CGAGATGGAG CTTGGACCAA CCCAGTGGGC ACTTGCCTTT TCCACAACAA TGGCCGAGCC 
GTGCACTACA ACGGCTCCAG TCTGCTCCAG TGGAAGAGCG TTCGCTTAGA CGTGACTCTC 
TCTCCTGGTG ACGTGGCAGG AATTGGCTGG GAGAGAACCG AGGGTACTCC ACCTCCACCG 
GGGCAGCCAG CCAAGGGCCG GGTGTACTTC ACATATTGCG GGCAGCGCCT TTCACCATAC 
CTTGAGGATG TCTCTGGTGG GATGTGGCCT GTGGTTCACA TTCAGAAAAA GAACACCAAA 
ACTCGAGCCA ACTTTGGCTC CCGCCCATTT GCCTATGCCG AGGGGCAGGC CCATCGCAAC 
GCAGCTGACC TATGCACTGA CCTGGCAGAG GAGATTAGCG CAAACTTCGA GGCCCTGCCC 
TTTGCCATGG CATCTGACAG TGACAACGAT GCCGGCACCA GTATTGCATC AGACCCAGGC 
ACTCATGGGC CACCGTGCCG GATTGCAGCC GTGGCCACCG CTCAGCAACA ATACGATAGT 
GACACCTCCT GTCATTATAA AGTTGAGCTC AGCTATGAGA ATTTCATCAC CTCTGGCCCA 
GACCCTCACC CGCCTCCCAT TGCAGACGAT GAGAGTGATG ATGATGACGA TGATGACATT 
CCACAGGAGG ACCACTATGC CCTGCTGGTG AAAGCTTGGG AGACCAAGGT TTTTCCCACC 
ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG AGGAAATCCG GCCTGGACCA GATCAAGGGT 
GCTTTACAAC TAGGCATGGT GGATATTGCC CGGCAGACGG TGGAATTTCT ATATGAGGAG 
AATGGCGGCA TCCCAAGAGA CCTGTATCTT CCCACCATTG AGGATATTAA AGATGAAGCA 
AACAAGTTCA CAATTGACAA AGTTCGAAAA GGTCTCACAG TTGTAACTCG CTCTCCAGAC 
AGTAATAATG TAGCCAGCAG TACCGTTGGA ACTGCTTTGC CAAAATTTGC CATCCGTGGG 
ATGCTGAAAA CCTTTGGACT TCATGGAGTC GTCTTAGATG TCGATTCAGT GAACGAGCTG 
GTGCAGGTGG AAACATATCT CCGGAGTGAG GGGGTGCTGG TGCGGTATTG GTACCCCATT 
GACATGTTGG AAAGGCCCCC TGCAGGCTAC CGAAGGACTG CCACCAATGG GCTGGTCACA 
CTGGACAACA CCAACCTTCA AATTCACAGG GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC 
AGGCTCTACT GCCGCATGGC CCTACTCAAC ATCTTCGCCC CCAAGTTCCC TCACTTGTTT 
ACCCGCCTCT TCCACATCCC TGCCATCCGA GACATCACTC TGGAGCACCT GCAACTGCTG 
TCCAATCAGC TCCTCGCCCC TCCCCTCCCA GATGGCACCA TCAGCTCCAG CTCCATCCTC 
CTGGCGCAGT CCCTGCAGCA CTGCATACAC TCCCAGAACT GTTCCGCCAC GGACCTCTTT 
TACCAGGGCA GCTCCCAGAC AGTGAGAGAG TGGCTGAGTG TGGCCATCAC CCGCACCCTG 
CACCAGGGCG AGGAGAGCCT TCTGGAGCTC ACGAGGCAGA TCTGCTCCTT CCTGCAGACA 
GCACCAGAGC AGTTCCCCTC CGAAGAATTC CCGATATCCG AATCCAAAGT CAATATGGAT 
GTTAATTTTC CCGGGGCGGC TTTTGTTGTG GTGTCTTGTA AAGAAAGTCA ATCTGGATTC 
CGCAAAGACT CCTCTTTGTA CAAGGCACCC TGGGCACGCG TCCTCGTGTA CGGGCTTGGC 
CACAAAGTGA AGCGAACCGG CCAGCTGAAC CTCATCGAGG CTGCCTGTTA CCCACGGGAT 
GCGTCCCCGG CCAACACTGG GCTTGCACCT CCGCCCACAG CTGACCAGTA CCCTTCTGTG 
GTGCTCTCCA CAGACAGGGT GCACATCAAG CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG 
CTGGTATTGC ATTCTCTGCC CCTGGAGTTC CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG 
CTGTCATGGA AGTCGGAGGA TGGCGAAGGG AAGTCTGAAG ATGAGCCTGA CACTATTCCC 
ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG CTAGGAAATT TCCTGTGGAC CACAGACATG 
GCAGCCTGCG TGAAGGAACT TGTCTTCCAC CTCCTGGCAG AGCTCCTGCG CACGGTGCAC 
GCACTAGAGC AGAGGAAGCA CCCCGCCGGC CTGTCCTCCT CCATTGCCCT GCAGCTCAAC 
CCCTGTCTGG CCATGCTGAT GGCCCTGCAG TCAGAGCTGC ACAAGCTGTA TGACGAGGAG 
ACCCAGAGCT GGGTCTCAGG CAGCACCTGT GGGGGCTCTG GGGCAGTGGC AACCGGCGAC 
CAGGGCAGGT TCTCCACGTA CTTTCATGCA CTCATGGAAG GGTGCCTGGC AGTTGCCGAA 
GTGACCCTGC CTACCAACAT GAGTGTCACA GCCAGTGGAG TGACCTCAGC AACCACCCCA 
AACCTCAGTG ACTCGTCGTC CTCCTCCTCT TCTTCTCCAG GACAGACACC GCAGAGTCCC 
AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG 
AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC GACTCCGCCG TGCTCAGCAT CGGGGGCAGC 
AAGCCTGAGG ACATGCTCTG GTTCCACCGC GCGCTCACCT TGCTCATCAT TCTGCGGCAC 
CTCACCAGGA AGGACCCCCA GGGCCTGGGT GTGACCAGCG ATGCCGTTGC AGATGCCTGC 
CAGGCCCTAG TGGGCCCCAC TGCCCATAGC CGCCTGCTGG TGATCTCTGG CATCCCTACC 
CACCTGGACG AGGGCATCGT CAGAGGTGCT ATCCGCAAGG CCTGCAATGC CCACGGCGGG 
GTCTTCAAAG ATGAGATCTA CATTCCACTG CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC 
AAGGCCGAAG GTGGCGATGG GAAGGCCGAG CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA 
GACAGCCTGG AGGGGAGCAC GTCCAGCAGC CTGGCCCCGG CCATGAGCAT CAGTGCCTCT 
GCCTCCACCA GCCAAGCCTC TGTCTGCAGC TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC 
CTCTCGGTGG ACCCACTGCC GTCCGGCCTG GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG 
CCCCACGTGG TGTCCAGCCA GGAGAGCCTG GATATTTCCC TGTGCAGCAC TGGCAGCCTG 
GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA GACAATGCGG AGACAGCCTC GGTGTCAGAC 
ATGGGCTCCA TGTACACAGT CACTTCCCTG GACAACCAGT CCCTTGCGGC ACGCCCCATC 
AAAGGCTTTG CCGTCGTGGA GATAAGATCC CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT 
TTATTTAACA ATAATGATTT AATTGGCTTA TCAAGTTTGG ATGGTGAAGA TGAATTGATG 
GAAATGTCCA CAGAAGAGAT TCTGACTGTA TCTGTAGTGA ACCAGAGTTT GTTTGATACT 
CAAGGGAGCC CGGGGTTAGA AGATTATTTC AATGATAAGT CAATTAAAGG TGAGAAGCTG 
GTGCCTGGGG CCAGAGAGGT CCTGACAGAG ATATTTAAGA GCTGTGCCCA CTCAGAGCAG 
ATGCTGAGCC TGACACCAGC AAAGCCCATC AGGGTCTCTG ACATATATCT CAGCAAGGAG 
CAGATCAACT CCCAGACTCC AGGCAACCTC CTCCACATCT TCTTCACCAA TGTCCGGCCT 
CCCAAGAAGG TGCTAGAAGA CCAGCTCACC CAGATCCTGA GGAAATATGG CGTGCCGAAG 
CCCAAGTTTG ACAAGAGCAA GTACAGCAAA GCTGGGAAGG AGCAGCACCC CGTGAAGGTG 
GTGAGCACCA AGCGGCCCAT CACCAAGCCG CCTGCCAAGG ACAAAGCCGT GCTCAACAGT 
GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA CCAACTGTGA AGCCCAAGTC ACCAGAAAAG 
AGCAAACCAG ACGAAAAGGA TCCAGAAAAG TCGCCCACAA AAAAACAAGA AGTCCCCGAG 
GAGAAGTACC TGACCTTAGA AGGGTTTCAC AAGTTTGTCA TCGACCGAGC CAAGCAGGAC 
ATCCGCAGCG TGTGGAGGGC AATTCTGTCT TGTGGCTACG ATCTTCATTT TGAGAGGTGT 
GCCTGCATTG ATGTCCGACA TGCGCAGAAG GCCTCAAGGA AGTGGACCCT GGAGATGGAT 
GTAGCTCTCG TGCAGTACAT CAATCGGCTG TGCCGCCACC TTGCCATCAC ACCTGCAAGG 
CTCCACCCCC ACGAGGTTTA CCTGGACCCT GCCGATGCCA CTGACCCCAG GGTGGCCTGT 
CTCTTGAATG TGCCCATTGA GAGCCTGCGC CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC 
ACCACGCTGG AGACCTTCTT CCTGCCCCTG GTGGAGCTGC GCCAGACACC CATGTACACC 
CACAGCATCG CGGCCCTTCT GAAGGAGGCC AAAGGGCTGA TCTTCTATGA CACAAAGGTG 
ACCGTGATGA ACCGAGTGCT GAATGCCACT GTGCAGAGGA CAGCTGACCA CGCGGCACCC 
GAGATCACCT TGGACCCATT GGAAATTGTG GGAGGGGAAA TCAGAGCTTC TGAAAACTCC 
TACTTCTGCC AGGCTGCCAG GCAACTGGCC TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG 
CTGGCGAGTG GCGGCGACCC CACCTACGCC TTCAACATCC GCTTCACCGG GGAGGAGGTC 
CACGGCACCA GTGGCTCCTT CCGCCACTTC CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC 
TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC AGCTCTGCTG TCAACAAGAA CAAGGGCAAG 
TACATCCTGA CGCCCAGCCC CATCACCTAC GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC 
CAGTTGCTGG GGATCGCCAT CCGGGCAGAT GTCCCCCTTC CCCTGGACCT CCTGCCCTCC 
TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG GACCCTGACC AGGACCTGCA GGAAGCAGAC 
ATCCTTACCT ATAATTACGT CAAGAAGTTT GAGAGCATCA ACGACGAGAG CGAGCTGGAA 
GCCCTGTGCG CCGAGATTGC CTCCCAGCAC CTGGCCACCG AGAGTCCTGA CGGCCCCAGC 
AAGCCCTGCT GCAGGTTCAC CTACCTGACC ATGACGGGCG AGGAGGTGGA GCTGTGCAGC 
CGCGGCCGGC ACATCCCTGT GGCGTGGGAG AACAAGGACA TCTACGCTGC AGCCATCCGC 
AGCCTGCGCT TGCGGGAGCT GCAGAACGTG GAATGCGTGA CAGCCGTGCG CGCAGGCCTG 
GGCTCTATCA TCCCCCTGCA GCTGCTCACC ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC 
ACCTGTGGCC TCCCCTACAT CAACCTCGAG TTCCTGAAGG CCCATACCAT GTACCAGGTG 
GGGCTAATGG AGACGGACCA ACACATCGAG TTCTTCTGGG GGGCCCTGGA GACGTTCACC 
CAGGAGGAGC TGTGCAGGTT CATCAAGTTC GCCTGCAACC AGGAGCGCAT CCCCTTCACC 
TGCCCCTGTA AAGATGGGGG CCCCGACACA GCCCACGTAC CCCCCTACCC CATGAAGATC 
GCCCCCCCAG ATGGCACAGC AGGTTCCCCA GACTCCCGCT ACATCCGGGT GGAGACCTGC 
ATGTTCATGA TCAAGCTCCC CCAGTACTCC TCTTTGGAAA TCATGCTGGA GAAACTGCGC 
TGTGCCATTC ACTACAGGGA AGACCCCCTA AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013219021.1
CDS	250..13068
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_013363567.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013363567.2

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT TGGTGATATA TGCGGGGCCT AACACAAACA GTAGGAAGGT TGCTGAATAT 
GGAGGCAATA CTCTGGGATA TGGCAGCCGT AGTGTCTTAG GAACTGGTTG GCCGAAGGAC 
TTGGTGAAGG TGGAAGGAGA TACAGTCACC TTCTCCTTTG AAATGAGAAG TGGCCGTGAA 
CACAACACTC CTGATAAAGC TATGTGGGGC TTTGCGTGCA CAGTTCGCGC TCAGGAGTCC 
TCGGAGGACG TCTCAGGAGG CCTGCCTTTT CTGGTGGACC TGGCTTTAGG TCTGTCTGTG 
TTAGCTTGTT CCATGTTAAG AATCCTGTAC AATGGACCAG AAATTACCAA AGAAGAGGAA 
GCCTGTCAGG AGCTTTTGCG GTCCAAACTT TTACAAAGGT GCCAGTGGCA GGTGGAGGCC 
AATGGCGTCA TCTCGCCTGC CCTCACCCCA AGTCCCTCTC CACTGCCTCT GACCATAGAG 
GAAGACCGAG AGTTCACCTA CCCCTCGGAT GTCCTCGTGC CTCCTGTTGG GAACTACTTT 
GACCTGCCCC GGATCCGGCT GCCTCCAGGA ATCATGATAA AGCTCAGGGA AATTTCTGGG 
CGAGCTAGAC CTCAATTTAG ACCAAGTATA AAGGAAGTGA TTCAGCCAGA TGTGATGGAG 
GAAATGGTGG TATCATGTGT TATTAAGCAC TTGAACTTGG TCGATGCACT GCAATCCCTA 
ATAAATTTCC AATATCAAGA AGAACATGCT GAGGAATATG ACTTACTATG TAAAATTATG 
GGAGAGACCT TTAAGAAACT CAATGCCATG GAGAGACAGC TGCAGAGTGT TGCAGAACTG 
GAACAGAAGT GGCAAAGTGA GGTTGATGAT GCCATGCAGG GGAAATTGGA GAACAACATG 
CCTTTCTTTT ATGATTACCA TTTTAATGAG AACAAAATGA AAGAACTAGA ACTTTTGTGT 
TCAATGAAGG AAGTCTCCTT TGATGGAAAT GATCTTGAAA ACATGGTCCT ATCACTGAGG 
GAGAAGTTCC TACAAGAAGT GAATTCTCTT ATTCAGAAGC CCTCTCATCC ACTGGCTAAA 
ACGAAGACAT TAGTGAAGAG TCTAATGAAC CGTGCTGAGC TGCTGCTGCA TGTCACCATT 
GCGGCCCAAT CTGGCCTCAC CAGGAGCATC TCGGGAACCC CTGCAGAGAC ACCGGCTTGT 
AAATCAGCTT CTGAAACAAA GGTGATATCT CATGCCGTCC GGCAGCCTGT TTTTCTCCGT 
AGTATGTCGG CTCCTTCTGA TCTAGAAATG ATTGGTAATG AAGATTTGGA ATTTACTAGA 
GCAAGTCAGA GGCGTCGACA TGTGACCAGC CACCGGAGCA GCTCCTTTAC ACTCCTGCAA 
TCGTTGGCCA TTGAGGACAG CAGGGACAAG CCCACCTATA GTGTCCTGCT GGGGCAGCTG 
TTTGCTTTCA TTGGCACCAA TCCCGACCAA GCTGTGTCCA GCAGCAGCTT CCTTCTTGCT 
GCACAGACGA GGTGGCGGAG GGGAAACACA CGCAAGCAGG CCCTGGTGCA CATGCGAGAG 
CTGCTCACAG CTGCCGTGCG CGTTGGGGGA GTGACACATC TTGTGGGGCC AGTGACTATG 
GTCCTCCAGG GAGGACCCAG GATTGAAGAG CTCACCTGTG GTGGGATGGT TGAGCAGGTT 
CAGGAAGCCT TTGGCGAAAC CATGACTTCG GTTGTGTCTC TGTGTGCCCG TTACCCTATC 
GCTTGTGCAA ATAGCATTGG ACTCTTGTGT ACCATACCTT ATACCAGGAG TGAGGAGAAG 
TGCCTGGTGC GCAGTGGCCT TGTCCAGCTC ATGGATCGAC TGTGCAGCTT GAGCAGTCAG 
ACCGAGTCCA GCTCCAGTGA GAAACAAACC AAGAAGCAGA AAGTGGCCAC CATGGCCTGG 
GCTGCCTTCC AGGTGCTGGC CAACCGCTGT GTCGAGTGGG AAAAAGAGGA AGGTGGCTCC 
ACAGAGGCTG TGCACTCGGG GCTGGCTCGG CAGGTGTCCA GCCTTCTCAC GAATCATCTT 
GCCCGAGCCA CTGAGTGCTG TGGCAACCAG GCTGCAGGAA ATGATGCTCT GCAGGATGTC 
CTGAGTCTGC TCAACGACCT CTCGAGGAGC CACATAGGGA AAGCCATCCT GAGCCAGCCA 
GCTTGTGTGT CCAAGCTCCT CTCCCTGCTG CTTGACCAGC GCCCATCTCC AAAATTGGTC 
CTTATCATTC TTCAACTGTG CCGGGCGGCA CTGCCATTGA TGAGCGTAGA GGACTGTGGA 
AATGTGGAAC TCCCGCCATG GAGCTATTCT GTCCCCTCCC TAAACAGTGA GCAGGAGGAT 
CCCAGTGACC CAGCTTCCAA GATCGCCTCC TTGCTCTTAG CAAAGCTGGC AGATTACGTG 
GTCCCAGGAT GTCAGACAGT TCTTTCTCCA ACTGCTTCTG AACCGGATAC CACATTAACA 
AAAACCAGTC CCAAGAATTC TTTAAAAGGA GATAAAGATC CTGGAGAAGA GAGTGAAGCA 
GTGGACGGCA AGCTCTCCAT ATTTATCCAC AAACGGGAAG ACCAGTCCTC CCACGAAGTT 
CTCCAACCAT TACTAAGTAG CTCAGAAGGG CGGCCCTTCC GACTTGGTAC TGGTGCCAAC 
ATGGAGAAAG TTGTGAAGAT GGATAGAGAC ATGACCAAGG GTGGCTGTTG CGAAGTGATC 
ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA GCCACCAAGT GGGCACAGTC AGGCCTCATC 
GTCAGCATTG GGCCACCAGT AGAGTCTGTC AATCCAGAGA CTGTGAGTGG ACTATCCACA 
GGTGACAAAA AGAAAACTGC CCAAACCTCC ATCTGCAGAG AGAGGAACTC AGAACTCGCC 
AGGACTGACC CTGTCCGACC CTTCATCAGT GGGCATGTGG CAAACAGCAT GGCTGCAGAA 
GTGATCGCTT TGCTTCATAG CTTGCTAATG GCACCTGAAT CTAACGCGGC TCAAATATGG 
ACCACAACAG CAGAAAAGGT TCTGTCTCGT GCATTGATGT ACATTCCACA ACTGGGGAAA 
CATGCAGAAA GCATTCTGGA AAATGGCAGC AGCAGTGGCA GAAAACTTGC CAAACTTCAG 
AGAATTGCTC GCCAGGCTGT CGCTGCACTG TGTGCACTTG GAGGCTTCAA AGAGACAATC 
AAGATAGGAT CTGAGGTTCA GGTTTTAGGT AGAGGAATAT CAGGAAGCAT TGGAGTAGTG 
GCTTCTATCA ACGAACAGGA AGGTATCGCT ACAGTCAGAT TTCCACCCAT AGACTGTCGA 
AAGACTTCTC AAGCGTCAGA CACATTGACT ATTCCATTGT CTAGACTTTG TGTTCCAAGG 
TCAGAGGCAC TGCCCCTCCA CAAACTGTCC ATTACTGAAA AGGTGGTACA GGCAGTTCAG 
TCCATGCTGC TTCCACAGGA AGGCAGTCTC TCAATTCACA CCTCGCTTCC TGCAACAGGA 
GATGGCTCTG CACCTGTGAT GGCAGTCGTC CGGCTCCTGG CTGAAATAAG AACAAGAGCA 
TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC AGCTTATTTT GTGAAGAATT CATACAGCAG 
TGTCCAGCAG CTGTTGAAGT TCTTAATCTG GTAGCCCAGG AATGCAGTGC TGGAGAGCGA 
CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG CTGAGGATGC TCTATCGGGA CTGCGCCCGG 
CCCCCGCCCC CTCCCCTGCA GGCTGACCGG AGACAGCCCA AAGAGATAAC TTGGAGCCCC 
TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC ATGTTCTCAT CTCACCTGAC CTCGGTCACA 
TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT CTGCCCCGGG GCACCTTTAT CTACGCCACC 
TCACCTCTGC CAGTTCAGGC CCCATCTTTT TACTGGGAAA TTGAAATTGT TTCCTATGGA 
GATACTGATG ACGACACTGG ACCAATAGTT TCCTTTGGCT TTGCTACAGA AGCCGAGAAA 
CGAGATGGAG CTTGGACCAA CCCAGTGGGC ACTTGCCTTT TCCACAACAA TGGCCGAGCC 
GTGCACTACA ACGGCTCCAG TCTGCTCCAG TGGAAGAGCG TTCGCTTAGA CGTGACTCTC 
TCTCCTGGTG ACGTGGCAGG AATTGGCTGG GAGAGAACCG AGGGTACTCC ACCTCCACCG 
GGGCAGCCAG CCAAGGGCCG GGTGTACTTC ACATATTGCG GGCAGCGCCT TTCACCATAC 
CTTGAGGATG TCTCTGGTGG GATGTGGCCT GTGGTTCACA TTCAGAAAAA GAACACCAAA 
ACTCGAGCCA ACTTTGGCTC CCGCCCATTT GCCTATGCCG AGGGGCAGGC CCATCGCAAC 
GCAGCTGACC TATGCACTGA CCTGGCAGAG GAGATTAGCG CAAACTTCGA GGCCCTGCCC 
TTTGCCATGG CATCTGACAG TGACAACGAT GCCGGCACCA GTATTGCATC AGACCCAGGC 
ACTCATGGGC CACCGTGCCG GATTGCAGCC GTGGCCACCG CTCAGCAACA ATACGATAGT 
GACACCTCCT GTCATTATAA AGTTGAGCTC AGCTATGAGA ATTTCATCAC CTCTGGCCCA 
GACCCTCACC CGCCTCCCAT TGCAGACGAT GAGAGTGATG ATGATGACGA TGATGACATT 
CCACAGGAGG ACCACTATGC CCTGCTGGTG AAAGCTTGGG AGACCAAGGT TTTTCCCACC 
ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG AGGAAATCCG GCCTGGACCA GATCAAGGGT 
GCTTTACAAC TAGGCATGGT GGATATTGCC CGGCAGACGG TGGAATTTCT ATATGAGGAG 
AATGGCGGCA TCCCAAGAGA CCTGTATCTT CCCACCATTG AGGATATTAA AGATGAAGCA 
AACAAGTTCA CAATTGACAA AGTTCGAAAA GGTCTCACAG TTGTAACTCG CTCTCCAGAC 
AGTAATAATG TAGCCAGCAG TACCGTTGGA ACTGCTTTGC CAAAATTTGC CATCCGTGGG 
ATGCTGAAAA CCTTTGGACT TCATGGAGTC GTCTTAGATG TCGATTCAGT GAACGAGCTG 
GTGCAGGTGG AAACATATCT CCGGAGTGAG GGGGTGCTGG TGCGGTATTG GTACCCCATT 
GACATGTTGG AAAGGCCCCC TGCAGGCTAC CGAAGGACTG CCACCAATGG GCTGGTCACA 
CTGGACAACA CCAACCTTCA AATTCACAGG GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC 
AGGCTCTACT GCCGCATGGC CCTACTCAAC ATCTTCGCCC CCAAGTTCCC TCACTTGTTT 
ACCCGCCTCT TCCACATCCC TGCCATCCGA GACATCACTC TGGAGCACCT GCAACTGCTG 
TCCAATCAGC TCCTCGCCCC TCCCCTCCCA GATGGCACCA TCAGCTCCAG CTCCATCCTC 
CTGGCGCAGT CCCTGCAGCA CTGCATACAC TCCCAGAACT GTTCCGCCAC GGACCTCTTT 
TACCAGGGCA GCTCCCAGAC AGTGAGAGAG TGGCTGAGTG TGGCCATCAC CCGCACCCTG 
CACCAGGGCG AGGAGAGCCT TCTGGAGCTC ACGAGGCAGA TCTGCTCCTT CCTGCAGACA 
GCACCAGAGC AGTTCCCCTC CGAAGAATTC CCGATATCCG AATCCAAAGT CAATATGGAT 
GTTAATTTTC CCGGGGCGGC TTTTGTTGTG GTGTCTTGTA AAGAAAGTCA ATCTGGATTC 
CGCAAAGACT CCTCTTTGTA CAAGGCACCC TGGGCACGCG TCCTCGTGTA CGGGCTTGGC 
CACAAAGTGA AGCGAACCGG CCAGCTGAAC CTCATCGAGG CTGCCTGTTA CCCACGGGAT 
GCGTCCCCGG CCAACACTGG GCTTGCACCT CCGCCCACAG CTGACCAGTA CCCTTCTGTG 
GTGCTCTCCA CAGACAGGGT GCACATCAAG CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG 
CTGGTATTGC ATTCTCTGCC CCTGGAGTTC CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG 
CTGTCATGGA AGTCGGAGGA TGGCGAAGGG AAGTCTGAAG ATGAGCCTGA CACTATTCCC 
ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG CTAGGAAATT TCCTGTGGAC CACAGACATG 
GCAGCCTGCG TGAAGGAACT TGTCTTCCAC CTCCTGGCAG AGCTCCTGCG CACGGTGCAC 
GCACTAGAGC AGAGGAAGCA CCCCGCCGGC CTGTCCTCCT CCATTGCCCT GCAGCTCAAC 
CCCTGTCTGG CCATGCTGAT GGCCCTGCAG TCAGAGCTGC ACAAGCTGTA TGACGAGGAG 
ACCCAGAGCT GGGTCTCAGG CAGCACCTGT GGGGGCTCTG GGGCAGTGGC AACCGGCGAC 
CAGGGCAGGT TCTCCACGTA CTTTCATGCA CTCATGGAAG GGTGCCTGGC AGTTGCCGAA 
GTGACCCTGC CTACCAACAT GAGTGTCACA GCCAGTGGAG TGACCTCAGC AACCACCCCA 
AACCTCAGTG ACTCGTCGTC CTCCTCCTCT TCTTCTCCAG GACAGACACC GCAGAGTCCC 
AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG 
AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC GACTCCGCCG TGCTCAGCAT CGGGGGCAGC 
AAGCCTGAGG ACATGCTCTG GTTCCACCGC GCGCTCACCT TGCTCATCAT TCTGCGGCAC 
CTCACCAGGA AGGACCCCCA GGGCCTGGGT GTGACCAGCG ATGCCGTTGC AGATGCCTGC 
CAGGCCCTAG TGGGCCCCAC TGCCCATAGC CGCCTGCTGG TGATCTCTGG CATCCCTACC 
CACCTGGACG AGGGCATCGT CAGAGGTGCT ATCCGCAAGG CCTGCAATGC CCACGGCGGG 
GTCTTCAAAG ATGAGATCTA CATTCCACTG CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC 
AAGGCCGAAG GTGGCGATGG GAAGGCCGAG CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA 
GACAGCCTGG AGGGGAGCAC GTCCAGCAGC CTGGCCCCGG CCATGAGCAT CAGTGCCTCT 
GCCTCCACCA GCCAAGCCTC TGTCTGCAGC TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC 
CTCTCGGTGG ACCCACTGCC GTCCGGCCTG GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG 
CCCCACGTGG TGTCCAGCCA GGAGAGCCTG GATATTTCCC TGTGCAGCAC TGGCAGCCTG 
GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA GACAATGCGG AGACAGCCTC GGTGTCAGAC 
ATGGGCTCCA TGTACACAGT CACTTCCCTG GACAACCAGT CCCTTGCGGC ACGCCCCATC 
AAAGGCTTTG CCGTCGTGGA GATAAGATCC CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT 
TTATTTAACA ATAATGATTT AATTGGCTTA TCAAGTTTGG ATGGTGAAGA TGAATTGATG 
GAAATGTCCA CAGAAGAGAT TCTGACTGTA TCTGTAGTGA ACCAGAGTTT GTTTGATACT 
CAAGGGAGCC CGGGGTTAGA AGATTATTTC AATGATAAGT CAATTAAAGG TGAGAAGCTG 
GTGCCTGGGG CCAGAGAGGT CCTGACAGAG ATATTTAAGA GCTGTGCCCA CTCAGAGCAG 
ATGCTGAGCC TGACACCAGC AAAGCCCATC AGGGTCTCTG ACATATATCT CAGCAAGGAG 
CAGATCAACT CCCAGACTCC AGGCAACCTC CTCCACATCT TCTTCACCAA TGTCCGGCCT 
CCCAAGAAGG TGCTAGAAGA CCAGCTCACC CAGATCCTGA GGAAATATGG CGTGCCGAAG 
CCCAAGTTTG ACAAGAGCAA GTACAGCAAA GCTGGGAAGG AGCAGCACCC CGTGAAGGTG 
GTGAGCACCA AGCGGCCCAT CACCAAGCCG CCTGCCAAGG ACAAAGCCGT GCTCAACAGT 
GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA CCAACTGTGA AGCCCAAGTC ACCAGAAAAG 
AGCAAACCAG ACGAAAAGGA TCCAGAAAAG TCGCCCACAA AAAAACAAGA AGTCCCCGAG 
GAGAAGTACC TGACCTTAGA AGGGTTTCAC AAGTTTGTCA TCGACCGAGC CAAGCAGGAC 
ATCCGCAGCG TGTGGAGGGC AATTCTGTCT TGTGGCTACG ATCTTCATTT TGAGAGGTGT 
GCCTGCATTG ATGTCCGACA TGCGCAGAAG GCCTCAAGGA AGTGGACCCT GGAGATGGAT 
GTAGCTCTCG TGCAGTACAT CAATCGGCTG TGCCGCCACC TTGCCATCAC ACCTGCAAGG 
CTCCACCCCC ACGAGGTTTA CCTGGACCCT GCCGATGCCA CTGACCCCAG GGTGGCCTGT 
CTCTTGAATG TGCCCATTGA GAGCCTGCGC CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC 
ACCACGCTGG AGACCTTCTT CCTGCCCCTG GTGGAGCTGC GCCAGACACC CATGTACACC 
CACAGCATCG CGGCCCTTCT GAAGGAGGCC AAAGGGCTGA TCTTCTATGA CACAAAGGTG 
ACCGTGATGA ACCGAGTGCT GAATGCCACT GTGCAGAGGA CAGCTGACCA CGCGGCACCC 
GAGATCACCT TGGACCCATT GGAAATTGTG GGAGGGGAAA TCAGAGCTTC TGAAAACTCC 
TACTTCTGCC AGGCTGCCAG GCAACTGGCC TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG 
CTGGCGAGTG GCGGCGACCC CACCTACGCC TTCAACATCC GCTTCACCGG GGAGGAGGTC 
CACGGCACCA GTGGCTCCTT CCGCCACTTC CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC 
TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC AGCTCTGCTG TCAACAAGAA CAAGGGCAAG 
TACATCCTGA CGCCCAGCCC CATCACCTAC GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC 
CAGTTGCTGG GGATCGCCAT CCGGGCAGAT GTCCCCCTTC CCCTGGACCT CCTGCCCTCC 
TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG GACCCTGACC AGGACCTGCA GGAAGCAGAC 
ATCCTTACCT ATAATTACGT CAAGAAGTTT GAGAGCATCA ACGACGAGAG CGAGCTGGAA 
GCCCTGTGCG CCGAGATTGC CTCCCAGCAC CTGGCCACCG AGAGTCCTGA CGGCCCCAGC 
AAGCCCTGCT GCAGGTTCAC CTACCTGACC ATGACGGGCG AGGAGGTGGA GCTGTGCAGC 
CGCGGCCGGC ACATCCCTGT GGCGTGGGAG AACAAGGACA TCTACGCTGC AGCCATCCGC 
AGCCTGCGCT TGCGGGAGCT GCAGAACGTG GAATGCGTGA CAGCCGTGCG CGCAGGCCTG 
GGCTCTATCA TCCCCCTGCA GCTGCTCACC ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC 
ACCTGTGGCC TCCCCTACAT CAACCTCGAG TTCCTGAAGG CCCATACCAT GTACCAGGTG 
GGGCTAATGG AGACGGACCA ACACATCGAG TTCTTCTGGG GGGCCCTGGA GACGTTCACC 
CAGGAGGAGC TGTGCAGGTT CATCAAGTTC GCCTGCAACC AGGAGCGCAT CCCCTTCACC 
TGCCCCTGTA AAGATGGGGG CCCCGACACA GCCCACGTAC CCCCCTACCC CATGAAGATC 
GCCCCCCCAG ATGGCACAGC AGGTTCCCCA GACTCCCGCT ACATCCGGGT GGAGACCTGC 
ATGTTCATGA TCAAGCTCCC CCAGTACTCC TCTTTGGAAA TCATGCTGGA GAAACTGCGC 
TGTGCCATTC ACTACAGGGA AGACCCCCTA AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734250.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589925.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X1, mRNA.

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CloneID	OIc83553
Clone ID Related Accession (Same CDS sequence)	XM_021734250.1
Accession Version	XM_021734250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGGTGAGCA CCAAGCGGCC CATCACCAAG 
CCGCCTGCCA AGGACAAAGC CGTGCTCAAC AGTGTCAGGA CTGCCTTAAG TGAGAAGAAA 
CCAACTGTGA AGCCCAAGTC ACCAGAAAAG AGCAAACCAG ACGAAAAGGA TCCAGAAAAG 
TCGCCCACAA AAAAACAAGA AGTCCCCGAG GAGAAGTACC TGACCTTAGA AGGGTTTCAC 
AAGTTTGTCA TCGACCGAGC CAAGCAGGAC ATCCGCAGCG TGTGGAGGGC AATTCTGTCT 
TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATTG ATGTCCGACA TGCGCAGAAG 
GCCTCAAGGA AGTGGACCCT GGAGATGGAT GTAGCTCTCG TGCAGTACAT CAATCGGCTG 
TGCCGCCACC TTGCCATCAC ACCTGCAAGG CTCCACCCCC ACGAGGTTTA CCTGGACCCT 
GCCGATGCCA CTGACCCCAG GGTGGCCTGT CTCTTGAATG TGCCCATTGA GAGCCTGCGC 
CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC ACCACGCTGG AGACCTTCTT CCTGCCCCTG 
GTGGAGCTGC GCCAGACACC CATGTACACC CACAGCATCG CGGCCCTTCT GAAGGAGGCC 
AAAGGGCTGA TCTTCTATGA CACAAAGGTG ACCGTGATGA ACCGAGTGCT GAATGCCACT 
GTGCAGAGGA CAGCTGACCA CGCGGCACCC GAGATCACCT TGGACCCATT GGAAATTGTG 
GGAGGGGAAA TCAGAGCTTC TGAAAACTCC TACTTCTGCC AGGCTGCCAG GCAACTGGCC 
TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG CTGGCGAGTG GCGGCGACCC CACCTACGCC 
TTCAACATCC GCTTCACCGG GGAGGAGGTC CACGGCACCA GTGGCTCCTT CCGCCACTTC 
CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC 
AGCTCTGCTG TCAACAAGAA CAAGGGCAAG TACATCCTGA CGCCCAGCCC CATCACCTAC 
GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC CAGTTGCTGG GGATCGCCAT CCGGGCAGAT 
GTCCCCCTTC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG 
GACCCTGACC AGGACCTGCA GGAAGCAGAC ATCCTTACCT ATAATTACGT CAAGAAGTTT 
GAGAGCATCA ACGACGAGAG CGAGCTGGAA GCCCTGTGCG CCGAGATTGC CTCCCAGCAC 
CTGGCCACCG AGAGTCCTGA CGGCCCCAGC AAGCCCTGCT GCAGGTTCAC CTACCTGACC 
ATGACGGGCG AGGAGGTGGA GCTGTGCAGC CGCGGCCGGC ACATCCCTGT GGCGTGGGAG 
AACAAGGACA TCTACGCTGC AGCCATCCGC AGCCTGCGCT TGCGGGAGCT GCAGAACGTG 
GAATGCGTGA CAGCCGTGCG CGCAGGCCTG GGCTCTATCA TCCCCCTGCA GCTGCTCACC 
ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC ACCTGTGGCC TCCCCTACAT CAACCTCGAG 
TTCCTGAAGG CCCATACCAT GTACCAGGTG GGGCTAATGG AGACGGACCA ACACATCGAG 
TTCTTCTGGG GGGCCCTGGA GACGTTCACC CAGGAGGAGC TGTGCAGGTT CATCAAGTTC 
GCCTGCAACC AGGAGCGCAT CCCCTTCACC TGCCCCTGTA AAGATGGGGG CCCCGACACA 
GCCCACGTAC CCCCCTACCC CATGAAGATC GCCCCCCCAG ATGGCACAGC AGGTTCCCCA 
GACTCCCGCT ACATCCGGGT GGAGACCTGC ATGTTCATGA TCAAGCTCCC CCAGTACTCC 
TCTTTGGAAA TCATGCTGGA GAAACTGCGC TGTGCCATTC ACTACAGGGA AGACCCCCTA 
AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589925.1
CDS	137..12985
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSLFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_021734250.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734250.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGGTGAGCA CCAAGCGGCC CATCACCAAG 
CCGCCTGCCA AGGACAAAGC CGTGCTCAAC AGTGTCAGGA CTGCCTTAAG TGAGAAGAAA 
CCAACTGTGA AGCCCAAGTC ACCAGAAAAG AGCAAACCAG ACGAAAAGGA TCCAGAAAAG 
TCGCCCACAA AAAAACAAGA AGTCCCCGAG GAGAAGTACC TGACCTTAGA AGGGTTTCAC 
AAGTTTGTCA TCGACCGAGC CAAGCAGGAC ATCCGCAGCG TGTGGAGGGC AATTCTGTCT 
TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATTG ATGTCCGACA TGCGCAGAAG 
GCCTCAAGGA AGTGGACCCT GGAGATGGAT GTAGCTCTCG TGCAGTACAT CAATCGGCTG 
TGCCGCCACC TTGCCATCAC ACCTGCAAGG CTCCACCCCC ACGAGGTTTA CCTGGACCCT 
GCCGATGCCA CTGACCCCAG GGTGGCCTGT CTCTTGAATG TGCCCATTGA GAGCCTGCGC 
CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC ACCACGCTGG AGACCTTCTT CCTGCCCCTG 
GTGGAGCTGC GCCAGACACC CATGTACACC CACAGCATCG CGGCCCTTCT GAAGGAGGCC 
AAAGGGCTGA TCTTCTATGA CACAAAGGTG ACCGTGATGA ACCGAGTGCT GAATGCCACT 
GTGCAGAGGA CAGCTGACCA CGCGGCACCC GAGATCACCT TGGACCCATT GGAAATTGTG 
GGAGGGGAAA TCAGAGCTTC TGAAAACTCC TACTTCTGCC AGGCTGCCAG GCAACTGGCC 
TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG CTGGCGAGTG GCGGCGACCC CACCTACGCC 
TTCAACATCC GCTTCACCGG GGAGGAGGTC CACGGCACCA GTGGCTCCTT CCGCCACTTC 
CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC 
AGCTCTGCTG TCAACAAGAA CAAGGGCAAG TACATCCTGA CGCCCAGCCC CATCACCTAC 
GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC CAGTTGCTGG GGATCGCCAT CCGGGCAGAT 
GTCCCCCTTC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG 
GACCCTGACC AGGACCTGCA GGAAGCAGAC ATCCTTACCT ATAATTACGT CAAGAAGTTT 
GAGAGCATCA ACGACGAGAG CGAGCTGGAA GCCCTGTGCG CCGAGATTGC CTCCCAGCAC 
CTGGCCACCG AGAGTCCTGA CGGCCCCAGC AAGCCCTGCT GCAGGTTCAC CTACCTGACC 
ATGACGGGCG AGGAGGTGGA GCTGTGCAGC CGCGGCCGGC ACATCCCTGT GGCGTGGGAG 
AACAAGGACA TCTACGCTGC AGCCATCCGC AGCCTGCGCT TGCGGGAGCT GCAGAACGTG 
GAATGCGTGA CAGCCGTGCG CGCAGGCCTG GGCTCTATCA TCCCCCTGCA GCTGCTCACC 
ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC ACCTGTGGCC TCCCCTACAT CAACCTCGAG 
TTCCTGAAGG CCCATACCAT GTACCAGGTG GGGCTAATGG AGACGGACCA ACACATCGAG 
TTCTTCTGGG GGGCCCTGGA GACGTTCACC CAGGAGGAGC TGTGCAGGTT CATCAAGTTC 
GCCTGCAACC AGGAGCGCAT CCCCTTCACC TGCCCCTGTA AAGATGGGGG CCCCGACACA 
GCCCACGTAC CCCCCTACCC CATGAAGATC GCCCCCCCAG ATGGCACAGC AGGTTCCCCA 
GACTCCCGCT ACATCCGGGT GGAGACCTGC ATGTTCATGA TCAAGCTCCC CCAGTACTCC 
TCTTTGGAAA TCATGCTGGA GAAACTGCGC TGTGCCATTC ACTACAGGGA AGACCCCCTA 
AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734251.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589926.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X2, mRNA.

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CloneID	OIc83554
Clone ID Related Accession (Same CDS sequence)	XM_021734251.1
Accession Version	XM_021734251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG CTCAGAAGGG 
CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAGAT GGATAGAGAC 
ATGACCAAGG GTGGCTGTTG CGAAGTGATC ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA 
GCCACCAAGT GGGCACAGTC AGGCCTCATC GTCAGCATTG GGCCACCAGT AGAGTCTGTC 
AATCCAGAGA CTGTGAGTGG ACTATCCACA GGTGACAAAA AGAAAACTGC CCAAACCTCC 
ATCTGCAGAG AGAGGAACTC AGAACTCGCC AGGACTGACC CTGTCCGACC CTTCATCAGT 
GGGCATGTGG CAAACAGCAT GGCTGCAGAA GTGATCGCTT TGCTTCATAG CTTGCTAATG 
GCACCTGAAT CTAACGCGGC TCAAATATGG ACCACAACAG CAGAAAAGGT TCTGTCTCGT 
GCATTGATGT ACATTCCACA ACTGGGGAAA CATGCAGAAA GCATTCTGGA AAATGGCAGC 
AGCAGTGGCA GAAAACTTGC CAAACTTCAG AGAATTGCTC GCCAGGCTGT CGCTGCACTG 
TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATAGGAT CTGAGGTTCA GGTTTTAGGT 
AGAGGAATAT CAGGAAGCAT TGGAGTAGTG GCTTCTATCA ACGAACAGGA AGGTATCGCT 
ACAGTCAGAT TTCCACCCAT AGACTGTCGA AAGACTTCTC AAGCGTCAGA CACATTGACT 
ATTCCATTGT CTAGACTTTG TGTTCCAAGG TCAGAGGCAC TGCCCCTCCA CAAACTGTCC 
ATTACTGAAA AGGTGGTACA GGCAGTTCAG TCCATGCTGC TTCCACAGGA AGGCAGTCTC 
TCAATTCACA CCTCGCTTCC TGCAACAGGA GATGGCTCTG CACCTGTGAT GGCAGTCGTC 
CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC 
AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAATCTG 
GTAGCCCAGG AATGCAGTGC TGGAGAGCGA CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG 
CTGAGGATGC TCTATCGGGA CTGCGCCCGG CCCCCGCCCC CTCCCCTGCA GGCTGACCGG 
AGACAGCCCA AAGAGATAAC TTGGAGCCCC TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC 
ATGTTCTCAT CTCACCTGAC CTCGGTCACA TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT 
CTGCCCCGGG GCACCTTTAT CTACGCCACC TCACCTCTGC CAGTTCAGGC CCCATCTTTT 
TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACTGATG ACGACACTGG ACCAATAGTT 
TCCTTTGGCT TTGCTACAGA AGCCGAGAAA CGAGATGGAG CTTGGACCAA CCCAGTGGGC 
ACTTGCCTTT TCCACAACAA TGGCCGAGCC GTGCACTACA ACGGCTCCAG TCTGCTCCAG 
TGGAAGAGCG TTCGCTTAGA CGTGACTCTC TCTCCTGGTG ACGTGGCAGG AATTGGCTGG 
GAGAGAACCG AGGGTACTCC ACCTCCACCG GGGCAGCCAG CCAAGGGCCG GGTGTACTTC 
ACATATTGCG GGCAGCGCCT TTCACCATAC CTTGAGGATG TCTCTGGTGG GATGTGGCCT 
GTGGTTCACA TTCAGAAAAA GAACACCAAA ACTCGAGCCA ACTTTGGCTC CCGCCCATTT 
GCCTATGCCG AGGGGCAGGC CCATCGCAAC GCAGCTGACC TATGCACTGA CCTGGCAGAG 
GAGATTAGCG CAAACTTCGA GGCCCTGCCC TTTGCCATGG CATCTGACAG TGACAACGAT 
GCCGGCACCA GTATTGCATC AGACCCAGGC ACTCATGGGC CACCGTGCCG GATTGCAGCC 
GTGGCCACCG CTCAGCAACA ATACGATAGT GACACCTCCT GTCATTATAA AGTTGAGCTC 
AGCTATGAGA ATTTCATCAC CTCTGGCCCA GACCCTCACC CGCCTCCCAT TGCAGACGAT 
GAGAGTGATG ATGATGACGA TGATGACATT CCACAGGAGG ACCACTATGC CCTGCTGGTG 
AAAGCTTGGG AGACCAAGGT TTTTCCCACC ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG 
AGGAAATCCG GCCTGGACCA GATCAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC 
CGGCAGACGG TGGAATTTCT ATATGAGGAG AATGGCGGCA TCCCAAGAGA CCTGTATCTT 
CCCACCATTG AGGATATTAA AGATGAAGCA AACAAGTTCA CAATTGACAA AGTTCGAAAA 
GGTCTCACAG TTGTAACTCG CTCTCCAGAC AGTAATAATG TAGCCAGCAG TACCGTTGGA 
ACTGCTTTGC CAAAATTTGC CATCCGTGGG ATGCTGAAAA CCTTTGGACT TCATGGAGTC 
GTCTTAGATG TCGATTCAGT GAACGAGCTG GTGCAGGTGG AAACATATCT CCGGAGTGAG 
GGGGTGCTGG TGCGGTATTG GTACCCCATT GACATGTTGG AAAGGCCCCC TGCAGGCTAC 
CGAAGGACTG CCACCAATGG GCTGGTCACA CTGGACAACA CCAACCTTCA AATTCACAGG 
GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC AGGCTCTACT GCCGCATGGC CCTACTCAAC 
ATCTTCGCCC CCAAGTTCCC TCACTTGTTT ACCCGCCTCT TCCACATCCC TGCCATCCGA 
GACATCACTC TGGAGCACCT GCAACTGCTG TCCAATCAGC TCCTCGCCCC TCCCCTCCCA 
GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGCATACAC 
TCCCAGAACT GTTCCGCCAC GGACCTCTTT TACCAGGGCA GCTCCCAGAC AGTGAGAGAG 
TGGCTGAGTG TGGCCATCAC CCGCACCCTG CACCAGGGCG AGGAGAGCCT TCTGGAGCTC 
ACGAGGCAGA TCTGCTCCTT CCTGCAGACA GCACCAGAGC AGTTCCCCTC CGAAGAATTC 
CCGATATCCG AATCCAAAGT CAATATGGAT GTTAATTTTC CCGGGGCGGC TTTTGTTGTG 
GTGTCTTGTA AAGAAAGTCA ATCTGGATTC CGCAAAGACT CCTCTTTGTA CAAGGCACCC 
TGGGCACGCG TCCTCGTGTA CGGGCTTGGC CACAAAGTGA AGCGAACCGG CCAGCTGAAC 
CTCATCGAGG CTGCCTGTTA CCCACGGGAT GCGTCCCCGG CCAACACTGG GCTTGCACCT 
CCGCCCACAG CTGACCAGTA CCCTTCTGTG GTGCTCTCCA CAGACAGGGT GCACATCAAG 
CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG CTGGTATTGC ATTCTCTGCC CCTGGAGTTC 
CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG CTGTCATGGA AGTCGGAGGA TGGCGAAGGG 
AAGTCTGAAG ATGAGCCTGA CACTATTCCC ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG 
CTAGGAAATT TCCTGTGGAC CACAGACATG GCAGCCTGCG TGAAGGAACT TGTCTTCCAC 
CTCCTGGCAG AGCTCCTGCG CACGGTGCAC GCACTAGAGC AGAGGAAGCA CCCCGCCGGC 
CTGTCCTCCT CCATTGCCCT GCAGCTCAAC CCCTGTCTGG CCATGCTGAT GGCCCTGCAG 
TCAGAGCTGC ACAAGCTGTA TGACGAGGAG ACCCAGAGCT GGGTCTCAGG CAGCACCTGT 
GGGGGCTCTG GGGCAGTGGC AACCGGCGAC CAGGGCAGGT TCTCCACGTA CTTTCATGCA 
CTCATGGAAG GGTGCCTGGC AGTTGCCGAA GTGACCCTGC CTACCAACAT GAGTGTCACA 
GCCAGTGGAG TGACCTCAGC AACCACCCCA AACCTCAGTG ACTCGTCGTC CTCCTCCTCT 
TCTTCTCCAG GACAGACACC GCAGAGTCCC AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG 
ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC 
GACTCCGCCG TGCTCAGCAT CGGGGGCAGC AAGCCTGAGG ACATGCTCTG GTTCCACCGC 
GCGCTCACCT TGCTCATCAT TCTGCGGCAC CTCACCAGGA AGGACCCCCA GGGCCTGGGT 
GTGACCAGCG ATGCCGTTGC AGATGCCTGC CAGGCCCTAG TGGGCCCCAC TGCCCATAGC 
CGCCTGCTGG TGATCTCTGG CATCCCTACC CACCTGGACG AGGGCATCGT CAGAGGTGCT 
ATCCGCAAGG CCTGCAATGC CCACGGCGGG GTCTTCAAAG ATGAGATCTA CATTCCACTG 
CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC AAGGCCGAAG GTGGCGATGG GAAGGCCGAG 
CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA GACAGCCTGG AGGGGAGCAC GTCCAGCAGC 
CTGGCCCCGG CCATGAGCAT CAGTGCCTCT GCCTCCACCA GCCAAGCCTC TGTCTGCAGC 
TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC CTCTCGGTGG ACCCACTGCC GTCCGGCCTG 
GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG CCCCACGTGG TGTCCAGCCA GGAGAGCCTG 
GATATTTCCC TGTGCAGCAC TGGCAGCCTG GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA 
GACAATGCGG AGACAGCCTC GGTGTCAGAC ATGGGCTCCA TGTACACAGT CACTTCCCTG 
GACAACCAGT CCCTTGCGGC ACGCCCCATC AAAGGCTTTG CCGTCGTGGA GATAAGATCC 
CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ATAATGATTT AATTGGCTTA 
TCAAGTTTGG ATGGTGAAGA TGAATTGATG GAAATGTCCA CAGAAGAGAT TCTGACTGTA 
TCTGTAGTGA ACCAGAGTTT GTTTGATACT CAAGGGAGCC CGGGGTTAGA AGATTATTTC 
AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT CCTGACAGAG 
ATATTTAAGA GCTGTGCCCA CTCAGAGCAG ATGCTGAGCC TGACACCAGC AAAGCCCATC 
AGGGTCTCTG ACATATATCT CAGCAAGGAG CAGATCAACT CCCAGACTCC AGGCAACCTC 
CTCCACATCT TCTTCACCAA TGTCCGGCCT CCCAAGAAGG TGCTAGAAGA CCAGCTCACC 
CAGATCCTGA GGAAATATGG CGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAA 
GCTGGGAAGG AGCAGCACCC CGTGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG 
CCTGCCAAGG ACAAAGCCGT GCTCAACAGT GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA 
CCAACTGTGA AGCCCAAGTC ACCAGAAAAG AGCAAACCAG ACGAAAAGGA TCCAGAAAAG 
TCGCCCACAA AAAAACAAGA AGTCCCCGAG GAGAAGTACC TGACCTTAGA AGGGTTTCAC 
AAGTTTGTCA TCGACCGAGC CAAGCAGGAC ATCCGCAGCG TGTGGAGGGC AATTCTGTCT 
TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATTG ATGTCCGACA TGCGCAGAAG 
GCCTCAAGGA AGTGGACCCT GGAGATGGAT GTAGCTCTCG TGCAGTACAT CAATCGGCTG 
TGCCGCCACC TTGCCATCAC ACCTGCAAGG CTCCACCCCC ACGAGGTTTA CCTGGACCCT 
GCCGATGCCA CTGACCCCAG GGTGGCCTGT CTCTTGAATG TGCCCATTGA GAGCCTGCGC 
CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC ACCACGCTGG AGACCTTCTT CCTGCCCCTG 
GTGGAGCTGC GCCAGACACC CATGTACACC CACAGCATCG CGGCCCTTCT GAAGGAGGCC 
AAAGGGCTGA TCTTCTATGA CACAAAGGTG ACCGTGATGA ACCGAGTGCT GAATGCCACT 
GTGCAGAGGA CAGCTGACCA CGCGGCACCC GAGATCACCT TGGACCCATT GGAAATTGTG 
GGAGGGGAAA TCAGAGCTTC TGAAAACTCC TACTTCTGCC AGGCTGCCAG GCAACTGGCC 
TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG CTGGCGAGTG GCGGCGACCC CACCTACGCC 
TTCAACATCC GCTTCACCGG GGAGGAGGTC CACGGCACCA GTGGCTCCTT CCGCCACTTC 
CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC 
AGCTCTGCTG TCAACAAGAA CAAGGGCAAG TACATCCTGA CGCCCAGCCC CATCACCTAC 
GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC CAGTTGCTGG GGATCGCCAT CCGGGCAGAT 
GTCCCCCTTC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG 
GACCCTGACC AGGACCTGCA GGAAGCAGAC ATCCTTACCT ATAATTACGT CAAGAAGTTT 
GAGAGCATCA ACGACGAGAG CGAGCTGGAA GCCCTGTGCG CCGAGATTGC CTCCCAGCAC 
CTGGCCACCG AGAGTCCTGA CGGCCCCAGC AAGCCCTGCT GCAGGTTCAC CTACCTGACC 
ATGACGGGCG AGGAGGTGGA GCTGTGCAGC CGCGGCCGGC ACATCCCTGT GGCGTGGGAG 
AACAAGGACA TCTACGCTGC AGCCATCCGC AGCCTGCGCT TGCGGGAGCT GCAGAACGTG 
GAATGCGTGA CAGCCGTGCG CGCAGGCCTG GGCTCTATCA TCCCCCTGCA GCTGCTCACC 
ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC ACCTGTGGCC TCCCCTACAT CAACCTCGAG 
TTCCTGAAGG CCCATACCAT GTACCAGGTG GGGCTAATGG AGACGGACCA ACACATCGAG 
TTCTTCTGGG GGGCCCTGGA GACGTTCACC CAGGAGGAGC TGTGCAGGTT CATCAAGTTC 
GCCTGCAACC AGGAGCGCAT CCCCTTCACC TGCCCCTGTA AAGATGGGGG CCCCGACACA 
GCCCACGTAC CCCCCTACCC CATGAAGATC GCCCCCCCAG ATGGCACAGC AGGTTCCCCA 
GACTCCCGCT ACATCCGGGT GGAGACCTGC ATGTTCATGA TCAAGCTCCC CCAGTACTCC 
TCTTTGGAAA TCATGCTGGA GAAACTGCGC TGTGCCATTC ACTACAGGGA AGACCCCCTA 
AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589926.1
CDS	137..12985
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSLFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_021734251.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734251.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG CTCAGAAGGG 
CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAGAT GGATAGAGAC 
ATGACCAAGG GTGGCTGTTG CGAAGTGATC ACAGAAGAGG CTGCAGCCGC CCTTCGGAAA 
GCCACCAAGT GGGCACAGTC AGGCCTCATC GTCAGCATTG GGCCACCAGT AGAGTCTGTC 
AATCCAGAGA CTGTGAGTGG ACTATCCACA GGTGACAAAA AGAAAACTGC CCAAACCTCC 
ATCTGCAGAG AGAGGAACTC AGAACTCGCC AGGACTGACC CTGTCCGACC CTTCATCAGT 
GGGCATGTGG CAAACAGCAT GGCTGCAGAA GTGATCGCTT TGCTTCATAG CTTGCTAATG 
GCACCTGAAT CTAACGCGGC TCAAATATGG ACCACAACAG CAGAAAAGGT TCTGTCTCGT 
GCATTGATGT ACATTCCACA ACTGGGGAAA CATGCAGAAA GCATTCTGGA AAATGGCAGC 
AGCAGTGGCA GAAAACTTGC CAAACTTCAG AGAATTGCTC GCCAGGCTGT CGCTGCACTG 
TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATAGGAT CTGAGGTTCA GGTTTTAGGT 
AGAGGAATAT CAGGAAGCAT TGGAGTAGTG GCTTCTATCA ACGAACAGGA AGGTATCGCT 
ACAGTCAGAT TTCCACCCAT AGACTGTCGA AAGACTTCTC AAGCGTCAGA CACATTGACT 
ATTCCATTGT CTAGACTTTG TGTTCCAAGG TCAGAGGCAC TGCCCCTCCA CAAACTGTCC 
ATTACTGAAA AGGTGGTACA GGCAGTTCAG TCCATGCTGC TTCCACAGGA AGGCAGTCTC 
TCAATTCACA CCTCGCTTCC TGCAACAGGA GATGGCTCTG CACCTGTGAT GGCAGTCGTC 
CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC 
AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAATCTG 
GTAGCCCAGG AATGCAGTGC TGGAGAGCGA CTTGCTGTTG TGGAGGTGCA GTGTGAGCGG 
CTGAGGATGC TCTATCGGGA CTGCGCCCGG CCCCCGCCCC CTCCCCTGCA GGCTGACCGG 
AGACAGCCCA AAGAGATAAC TTGGAGCCCC TCCCGAGTGT TTCCTCCAGT GCGGGCCTGC 
ATGTTCTCAT CTCACCTGAC CTCGGTCACA TTCCTGGCTG ACCCCAGCGC TGGGGGAGGT 
CTGCCCCGGG GCACCTTTAT CTACGCCACC TCACCTCTGC CAGTTCAGGC CCCATCTTTT 
TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACTGATG ACGACACTGG ACCAATAGTT 
TCCTTTGGCT TTGCTACAGA AGCCGAGAAA CGAGATGGAG CTTGGACCAA CCCAGTGGGC 
ACTTGCCTTT TCCACAACAA TGGCCGAGCC GTGCACTACA ACGGCTCCAG TCTGCTCCAG 
TGGAAGAGCG TTCGCTTAGA CGTGACTCTC TCTCCTGGTG ACGTGGCAGG AATTGGCTGG 
GAGAGAACCG AGGGTACTCC ACCTCCACCG GGGCAGCCAG CCAAGGGCCG GGTGTACTTC 
ACATATTGCG GGCAGCGCCT TTCACCATAC CTTGAGGATG TCTCTGGTGG GATGTGGCCT 
GTGGTTCACA TTCAGAAAAA GAACACCAAA ACTCGAGCCA ACTTTGGCTC CCGCCCATTT 
GCCTATGCCG AGGGGCAGGC CCATCGCAAC GCAGCTGACC TATGCACTGA CCTGGCAGAG 
GAGATTAGCG CAAACTTCGA GGCCCTGCCC TTTGCCATGG CATCTGACAG TGACAACGAT 
GCCGGCACCA GTATTGCATC AGACCCAGGC ACTCATGGGC CACCGTGCCG GATTGCAGCC 
GTGGCCACCG CTCAGCAACA ATACGATAGT GACACCTCCT GTCATTATAA AGTTGAGCTC 
AGCTATGAGA ATTTCATCAC CTCTGGCCCA GACCCTCACC CGCCTCCCAT TGCAGACGAT 
GAGAGTGATG ATGATGACGA TGATGACATT CCACAGGAGG ACCACTATGC CCTGCTGGTG 
AAAGCTTGGG AGACCAAGGT TTTTCCCACC ATCCGAAGAC GCTTCCGGAA TGAAGCAGAG 
AGGAAATCCG GCCTGGACCA GATCAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC 
CGGCAGACGG TGGAATTTCT ATATGAGGAG AATGGCGGCA TCCCAAGAGA CCTGTATCTT 
CCCACCATTG AGGATATTAA AGATGAAGCA AACAAGTTCA CAATTGACAA AGTTCGAAAA 
GGTCTCACAG TTGTAACTCG CTCTCCAGAC AGTAATAATG TAGCCAGCAG TACCGTTGGA 
ACTGCTTTGC CAAAATTTGC CATCCGTGGG ATGCTGAAAA CCTTTGGACT TCATGGAGTC 
GTCTTAGATG TCGATTCAGT GAACGAGCTG GTGCAGGTGG AAACATATCT CCGGAGTGAG 
GGGGTGCTGG TGCGGTATTG GTACCCCATT GACATGTTGG AAAGGCCCCC TGCAGGCTAC 
CGAAGGACTG CCACCAATGG GCTGGTCACA CTGGACAACA CCAACCTTCA AATTCACAGG 
GAGCTGCTGC GGTGTGAGGC TGCGTTGGCC AGGCTCTACT GCCGCATGGC CCTACTCAAC 
ATCTTCGCCC CCAAGTTCCC TCACTTGTTT ACCCGCCTCT TCCACATCCC TGCCATCCGA 
GACATCACTC TGGAGCACCT GCAACTGCTG TCCAATCAGC TCCTCGCCCC TCCCCTCCCA 
GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGCATACAC 
TCCCAGAACT GTTCCGCCAC GGACCTCTTT TACCAGGGCA GCTCCCAGAC AGTGAGAGAG 
TGGCTGAGTG TGGCCATCAC CCGCACCCTG CACCAGGGCG AGGAGAGCCT TCTGGAGCTC 
ACGAGGCAGA TCTGCTCCTT CCTGCAGACA GCACCAGAGC AGTTCCCCTC CGAAGAATTC 
CCGATATCCG AATCCAAAGT CAATATGGAT GTTAATTTTC CCGGGGCGGC TTTTGTTGTG 
GTGTCTTGTA AAGAAAGTCA ATCTGGATTC CGCAAAGACT CCTCTTTGTA CAAGGCACCC 
TGGGCACGCG TCCTCGTGTA CGGGCTTGGC CACAAAGTGA AGCGAACCGG CCAGCTGAAC 
CTCATCGAGG CTGCCTGTTA CCCACGGGAT GCGTCCCCGG CCAACACTGG GCTTGCACCT 
CCGCCCACAG CTGACCAGTA CCCTTCTGTG GTGCTCTCCA CAGACAGGGT GCACATCAAG 
CTAGGAGTGT CTCCCCCTCC TGGAGCCGTG CTGGTATTGC ATTCTCTGCC CCTGGAGTTC 
CCGCTGGCTA TGGCTTTCGC AGAGCAGCTG CTGTCATGGA AGTCGGAGGA TGGCGAAGGG 
AAGTCTGAAG ATGAGCCTGA CACTATTCCC ACCTCAGTCC TCCTGCAGGT GGTGGAGCTG 
CTAGGAAATT TCCTGTGGAC CACAGACATG GCAGCCTGCG TGAAGGAACT TGTCTTCCAC 
CTCCTGGCAG AGCTCCTGCG CACGGTGCAC GCACTAGAGC AGAGGAAGCA CCCCGCCGGC 
CTGTCCTCCT CCATTGCCCT GCAGCTCAAC CCCTGTCTGG CCATGCTGAT GGCCCTGCAG 
TCAGAGCTGC ACAAGCTGTA TGACGAGGAG ACCCAGAGCT GGGTCTCAGG CAGCACCTGT 
GGGGGCTCTG GGGCAGTGGC AACCGGCGAC CAGGGCAGGT TCTCCACGTA CTTTCATGCA 
CTCATGGAAG GGTGCCTGGC AGTTGCCGAA GTGACCCTGC CTACCAACAT GAGTGTCACA 
GCCAGTGGAG TGACCTCAGC AACCACCCCA AACCTCAGTG ACTCGTCGTC CTCCTCCTCT 
TCTTCTCCAG GACAGACACC GCAGAGTCCC AGCCTCCTCT CCAAGAGGAA GAAAGTCAAG 
ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGGCAGT CCTCCCGCAC AGTGGAGTCC 
GACTCCGCCG TGCTCAGCAT CGGGGGCAGC AAGCCTGAGG ACATGCTCTG GTTCCACCGC 
GCGCTCACCT TGCTCATCAT TCTGCGGCAC CTCACCAGGA AGGACCCCCA GGGCCTGGGT 
GTGACCAGCG ATGCCGTTGC AGATGCCTGC CAGGCCCTAG TGGGCCCCAC TGCCCATAGC 
CGCCTGCTGG TGATCTCTGG CATCCCTACC CACCTGGACG AGGGCATCGT CAGAGGTGCT 
ATCCGCAAGG CCTGCAATGC CCACGGCGGG GTCTTCAAAG ATGAGATCTA CATTCCACTG 
CAGGAAGAAG ACCCCAAGAA GCCCAAAGAC AAGGCCGAAG GTGGCGATGG GAAGGCCGAG 
CCGGAGAAGA CGCTGGGCTT CCCCAGTGCA GACAGCCTGG AGGGGAGCAC GTCCAGCAGC 
CTGGCCCCGG CCATGAGCAT CAGTGCCTCT GCCTCCACCA GCCAAGCCTC TGTCTGCAGC 
TCCCAGGGGG TCTCTCAGAC CGTCAGCGAC CTCTCGGTGG ACCCACTGCC GTCCGGCCTG 
GAGTTGCCCA TTCCTCCTGG CCTGCTGGAG CCCCACGTGG TGTCCAGCCA GGAGAGCCTG 
GATATTTCCC TGTGCAGCAC TGGCAGCCTG GGCAGTCTGG GCAGCCTGGG GGAGCCTCTA 
GACAATGCGG AGACAGCCTC GGTGTCAGAC ATGGGCTCCA TGTACACAGT CACTTCCCTG 
GACAACCAGT CCCTTGCGGC ACGCCCCATC AAAGGCTTTG CCGTCGTGGA GATAAGATCC 
CGAGCCAAGA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ATAATGATTT AATTGGCTTA 
TCAAGTTTGG ATGGTGAAGA TGAATTGATG GAAATGTCCA CAGAAGAGAT TCTGACTGTA 
TCTGTAGTGA ACCAGAGTTT GTTTGATACT CAAGGGAGCC CGGGGTTAGA AGATTATTTC 
AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT CCTGACAGAG 
ATATTTAAGA GCTGTGCCCA CTCAGAGCAG ATGCTGAGCC TGACACCAGC AAAGCCCATC 
AGGGTCTCTG ACATATATCT CAGCAAGGAG CAGATCAACT CCCAGACTCC AGGCAACCTC 
CTCCACATCT TCTTCACCAA TGTCCGGCCT CCCAAGAAGG TGCTAGAAGA CCAGCTCACC 
CAGATCCTGA GGAAATATGG CGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAA 
GCTGGGAAGG AGCAGCACCC CGTGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG 
CCTGCCAAGG ACAAAGCCGT GCTCAACAGT GTCAGCAGGA CTGCCTTAAG TGAGAAGAAA 
CCAACTGTGA AGCCCAAGTC ACCAGAAAAG AGCAAACCAG ACGAAAAGGA TCCAGAAAAG 
TCGCCCACAA AAAAACAAGA AGTCCCCGAG GAGAAGTACC TGACCTTAGA AGGGTTTCAC 
AAGTTTGTCA TCGACCGAGC CAAGCAGGAC ATCCGCAGCG TGTGGAGGGC AATTCTGTCT 
TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATTG ATGTCCGACA TGCGCAGAAG 
GCCTCAAGGA AGTGGACCCT GGAGATGGAT GTAGCTCTCG TGCAGTACAT CAATCGGCTG 
TGCCGCCACC TTGCCATCAC ACCTGCAAGG CTCCACCCCC ACGAGGTTTA CCTGGACCCT 
GCCGATGCCA CTGACCCCAG GGTGGCCTGT CTCTTGAATG TGCCCATTGA GAGCCTGCGC 
CTGCGCTTCG CTTTGCTGCA GTCCCTCAAC ACCACGCTGG AGACCTTCTT CCTGCCCCTG 
GTGGAGCTGC GCCAGACACC CATGTACACC CACAGCATCG CGGCCCTTCT GAAGGAGGCC 
AAAGGGCTGA TCTTCTATGA CACAAAGGTG ACCGTGATGA ACCGAGTGCT GAATGCCACT 
GTGCAGAGGA CAGCTGACCA CGCGGCACCC GAGATCACCT TGGACCCATT GGAAATTGTG 
GGAGGGGAAA TCAGAGCTTC TGAAAACTCC TACTTCTGCC AGGCTGCCAG GCAACTGGCC 
TCTGTGCCAT CCTCTCAGCT CTGCGTCAAG CTGGCGAGTG GCGGCGACCC CACCTACGCC 
TTCAACATCC GCTTCACCGG GGAGGAGGTC CACGGCACCA GTGGCTCCTT CCGCCACTTC 
CTGTGGCAGG TGTGCAAGGA GCTGCAGAGC TCTTCGCTGT CGCTCCTGCT GCTGTGCCCC 
AGCTCTGCTG TCAACAAGAA CAAGGGCAAG TACATCCTGA CGCCCAGCCC CATCACCTAC 
GGAGAGGAGC AGCTGCTGCA CTTCCTAGGC CAGTTGCTGG GGATCGCCAT CCGGGCAGAT 
GTCCCCCTTC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CTCTGGTGGG GGAGCCCCTG 
GACCCTGACC AGGACCTGCA GGAAGCAGAC ATCCTTACCT ATAATTACGT CAAGAAGTTT 
GAGAGCATCA ACGACGAGAG CGAGCTGGAA GCCCTGTGCG CCGAGATTGC CTCCCAGCAC 
CTGGCCACCG AGAGTCCTGA CGGCCCCAGC AAGCCCTGCT GCAGGTTCAC CTACCTGACC 
ATGACGGGCG AGGAGGTGGA GCTGTGCAGC CGCGGCCGGC ACATCCCTGT GGCGTGGGAG 
AACAAGGACA TCTACGCTGC AGCCATCCGC AGCCTGCGCT TGCGGGAGCT GCAGAACGTG 
GAATGCGTGA CAGCCGTGCG CGCAGGCCTG GGCTCTATCA TCCCCCTGCA GCTGCTCACC 
ACCCTCAGCC CCCTGGAGAT GGAGCTGCGC ACCTGTGGCC TCCCCTACAT CAACCTCGAG 
TTCCTGAAGG CCCATACCAT GTACCAGGTG GGGCTAATGG AGACGGACCA ACACATCGAG 
TTCTTCTGGG GGGCCCTGGA GACGTTCACC CAGGAGGAGC TGTGCAGGTT CATCAAGTTC 
GCCTGCAACC AGGAGCGCAT CCCCTTCACC TGCCCCTGTA AAGATGGGGG CCCCGACACA 
GCCCACGTAC CCCCCTACCC CATGAAGATC GCCCCCCCAG ATGGCACAGC AGGTTCCCCA 
GACTCCCGCT ACATCCGGGT GGAGACCTGC ATGTTCATGA TCAAGCTCCC CCAGTACTCC 
TCTTTGGAAA TCATGCTGGA GAAACTGCGC TGTGCCATTC ACTACAGGGA AGACCCCCTA 
AGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734252.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589927.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X3, mRNA.

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CloneID	OIc83555
Clone ID Related Accession (Same CDS sequence)	XM_021734252.1
Accession Version	XM_021734252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT CTAACAGTCT ATTTTCTGTT TTGAAGTTGG TGATATATGC GGGGCCTAAC 
ACAAACAGTA GGAAGGTTGC TGAATATGGA GGCAATACTC TGGGATATGG CAGCCGTAGT 
GTCTTAGGAA CTGGTTGGCC GAAGGACTTG GTGAAGGTGG AAGGAGATAC AGTCACCTTC 
TCCTTTGAAA TGAGAAGTGG CCGTGAACAC AACACTCCTG ATAAAGCTAT GTGGGGCTTT 
GCGTGCACAG TTCGCGCTCA GGAGTCCTCG GAGGACGTCT CAGGAGGCCT GCCTTTTCTG 
GTGGACCTGG CTTTAGGTCT GTCTGTGTTA GCTTGTTCCA TGTTAAGAAT CCTGTACAAT 
GGACCAGAAA TTACCAAAGA AGAGGAAGCC TGTCAGGAGC TTTTGCGGTC CAAACTTTTA 
CAAAGGTGCC AGTGGCAGGT GGAGGCCAAT GGCGTCATCT CGCCTGCCCT CACCCCAAGT 
CCCTCTCCAC TGCCTCTGAC CATAGAGGAA GACCGAGAGT TCACCTACCC CTCGGATGTC 
CTCGTGCCTC CTGTTGGGAA CTACTTTGAC CTGCCCCGGA TCCGGCTGCC TCCAGGAATC 
ATGATAAAGC TCAGGGAAAT TTCTGGGCGA GCTAGACCTC AATTTAGACC AAGTATAAAG 
GAAGTGATTC AGCCAGATGT GATGGAGGAA ATGGTGGTAT CATGTGTTAT TAAGCACTTG 
AACTTGGTCG ATGCACTGCA ATCCCTAATA AATTTCCAAT ATCAAGAAGA ACATGCTGAG 
GAATATGACT TACTATGTAA AATTATGGGA GAGACCTTTA AGAAACTCAA TGCCATGGAG 
AGACAGCTGC AGAGTGTTGC AGAACTGGAA CAGAAGTGGC AAAGTGAGGT TGATGATGCC 
ATGCAGGGGA AATTGGAGAA CAACATGCCT TTCTTTTATG ATTACCATTT TAATGAGAAC 
AAAATGAAAG AACTAGAACT TTTGTGTTCA ATGAAGGAAG TCTCCTTTGA TGGAAATGAT 
CTTGAAAACA TGGTCCTATC ACTGAGGGAG AAGTTCCTAC AAGAAGTGAA TTCTCTTATT 
CAGAAGCCCT CTCATCCACT GGCTAAAACG AAGACATTAG TGAAGAGTCT AATGAACCGT 
GCTGAGCTGC TGCTGCATGT CACCATTGCG GCCCAATCTG GCCTCACCAG GAGCATCTCG 
GGAACCCCTG CAGAGACACC GGCTTGTAAA TCAGCTTCTG AAACAAAGGT GATATCTCAT 
GCCGTCCGGC AGCCTGTTTT TCTCCGTAGT ATGTCGGCTC CTTCTGATCT AGAAATGATT 
GGTAATGAAG ATTTGGAATT TACTAGAGCA AGTCAGAGGC GTCGACATGT GACCAGCCAC 
CGGAGCAGCT CCTTTACACT CCTGCAATCG TTGGCCATTG AGGACAGCAG GGACAAGCCC 
ACCTATAGTG TCCTGCTGGG GCAGCTGTTT GCTTTCATTG GCACCAATCC CGACCAAGCT 
GTGTCCAGCA GCAGCTTCCT TCTTGCTGCA CAGACGAGGT GGCGGAGGGG AAACACACGC 
AAGCAGGCCC TGGTGCACAT GCGAGAGCTG CTCACAGCTG CCGTGCGCGT TGGGGGAGTG 
ACACATCTTG TGGGGCCAGT GACTATGGTC CTCCAGGGAG GACCCAGGAT TGAAGAGCTC 
ACCTGTGGTG GGATGGTTGA GCAGGTTCAG GAAGCCTTTG GCGAAACCAT GACTTCGGTT 
GTGTCTCTGT GTGCCCGTTA CCCTATCGCT TGTGCAAATA GCATTGGACT CTTGTGTACC 
ATACCTTATA CCAGGAGTGA GGAGAAGTGC CTGGTGCGCA GTGGCCTTGT CCAGCTCATG 
GATCGACTGT GCAGCTTGAG CAGTCAGACC GAGTCCAGCT CCAGTGAGAA ACAAACCAAG 
AAGCAGAAAG TGGCCACCAT GGCCTGGGCT GCCTTCCAGG TGCTGGCCAA CCGCTGTGTC 
GAGTGGGAAA AAGAGGAAGG TGGCTCCACA GAGGCTGTGC ACTCGGGGCT GGCTCGGCAG 
GTGTCCAGCC TTCTCACGAA TCATCTTGCC CGAGCCACTG AGTGCTGTGG CAACCAGGCT 
GCAGGAAATG ATGCTCTGCA GGATGTCCTG AGTCTGCTCA ACGACCTCTC GAGGAGCCAC 
ATAGGGAAAG CCATCCTGAG CCAGCCAGCT TGTGTGTCCA AGCTCCTCTC CCTGCTGCTT 
GACCAGCGCC CATCTCCAAA ATTGGTCCTT ATCATTCTTC AACTGTGCCG GGCGGCACTG 
CCATTGATGA GCGTAGAGGA CTGTGGAAAT GTGGAACTCC CGCCATGGAG CTATTCTGTC 
CCCTCCCTAA ACAGTGAGCA GGAGGATCCC AGTGACCCAG CTTCCAAGAT CGCCTCCTTG 
CTCTTAGCAA AGCTGGCAGA TTACGTGGTC CCAGGATGTC AGACAGTTCT TTCTCCAACT 
GCTTCTGAAC CGGATACCAC ATTAACAAAA ACCAGTCCCA AGAATTCTTT AAAAGGAGAT 
AAAGATCCTG GAGAAGAGAG TGAAGCAGTG GACGGCAAGC TCTCCATATT TATCCACAAA 
CGGGAAGACC AGTCCTCCCA CGAAGTTCTC CAACCATTAC TAAGTAGCTC AGAAGGGCGG 
CCCTTCCGAC TTGGTACTGG TGCCAACATG GAGAAAGTTG TGAAGATGGA TAGAGACATG 
ACCAAGGGTG GCTGTTGCGA AGTGATCACA GAAGAGGCTG CAGCCGCCCT TCGGAAAGCC 
ACCAAGTGGG CACAGTCAGG CCTCATCGTC AGCATTGGGC CACCAGTAGA GTCTGTCAAT 
CCAGAGACTG TGAGTGGACT ATCCACAGGT GACAAAAAGA AAACTGCCCA AACCTCCATC 
TGCAGAGAGA GGAACTCAGA ACTCGCCAGG ACTGACCCTG TCCGACCCTT CATCAGTGGG 
CATGTGGCAA ACAGCATGGC TGCAGAAGTG ATCGCTTTGC TTCATAGCTT GCTAATGGCA 
CCTGAATCTA ACGCGGCTCA AATATGGACC ACAACAGCAG AAAAGGTTCT GTCTCGTGCA 
TTGATGTACA TTCCACAACT GGGGAAACAT GCAGAAAGCA TTCTGGAAAA TGGCAGCAGC 
AGTGGCAGAA AACTTGCCAA ACTTCAGAGA ATTGCTCGCC AGGCTGTCGC TGCACTGTGT 
GCACTTGGAG GCTTCAAAGA GACAATCAAG ATAGGATCTG AGGTTCAGGT TTTAGGTAGA 
GGAATATCAG GAAGCATTGG AGTAGTGGCT TCTATCAACG AACAGGAAGG TATCGCTACA 
GTCAGATTTC CACCCATAGA CTGTCGAAAG ACTTCTCAAG CGTCAGACAC ATTGACTATT 
CCATTGTCTA GACTTTGTGT TCCAAGGTCA GAGGCACTGC CCCTCCACAA ACTGTCCATT 
ACTGAAAAGG TGGTACAGGC AGTTCAGTCC ATGCTGCTTC CACAGGAAGG CAGTCTCTCA 
ATTCACACCT CGCTTCCTGC AACAGGAGAT GGCTCTGCAC CTGTGATGGC AGTCGTCCGG 
CTCCTGGCTG AAATAAGAAC AAGAGCATGC CTGGTCATGG CCCAGCTTTT AGAAGACAGC 
TTATTTTGTG AAGAATTCAT ACAGCAGTGT CCAGCAGCTG TTGAAGTTCT TAATCTGGTA 
GCCCAGGAAT GCAGTGCTGG AGAGCGACTT GCTGTTGTGG AGGTGCAGTG TGAGCGGCTG 
AGGATGCTCT ATCGGGACTG CGCCCGGCCC CCGCCCCCTC CCCTGCAGGC TGACCGGAGA 
CAGCCCAAAG AGATAACTTG GAGCCCCTCC CGAGTGTTTC CTCCAGTGCG GGCCTGCATG 
TTCTCATCTC ACCTGACCTC GGTCACATTC CTGGCTGACC CCAGCGCTGG GGGAGGTCTG 
CCCCGGGGCA CCTTTATCTA CGCCACCTCA CCTCTGCCAG TTCAGGCCCC ATCTTTTTAC 
TGGGAAATTG AAATTGTTTC CTATGGAGAT ACTGATGACG ACACTGGACC AATAGTTTCC 
TTTGGCTTTG CTACAGAAGC CGAGAAACGA GATGGAGCTT GGACCAACCC AGTGGGCACT 
TGCCTTTTCC ACAACAATGG CCGAGCCGTG CACTACAACG GCTCCAGTCT GCTCCAGTGG 
AAGAGCGTTC GCTTAGACGT GACTCTCTCT CCTGGTGACG TGGCAGGAAT TGGCTGGGAG 
AGAACCGAGG GTACTCCACC TCCACCGGGG CAGCCAGCCA AGGGCCGGGT GTACTTCACA 
TATTGCGGGC AGCGCCTTTC ACCATACCTT GAGGATGTCT CTGGTGGGAT GTGGCCTGTG 
GTTCACATTC AGAAAAAGAA CACCAAAACT CGAGCCAACT TTGGCTCCCG CCCATTTGCC 
TATGCCGAGG GGCAGGCCCA TCGCAACGCA GCTGACCTAT GCACTGACCT GGCAGAGGAG 
ATTAGCGCAA ACTTCGAGGC CCTGCCCTTT GCCATGGCAT CTGACAGTGA CAACGATGCC 
GGCACCAGTA TTGCATCAGA CCCAGGCACT CATGGGCCAC CGTGCCGGAT TGCAGCCGTG 
GCCACCGCTC AGCAACAATA CGATAGTGAC ACCTCCTGTC ATTATAAAGT TGAGCTCAGC 
TATGAGAATT TCATCACCTC TGGCCCAGAC CCTCACCCGC CTCCCATTGC AGACGATGAG 
AGTGATGATG ATGACGATGA TGACATTCCA CAGGAGGACC ACTATGCCCT GCTGGTGAAA 
GCTTGGGAGA CCAAGGTTTT TCCCACCATC CGAAGACGCT TCCGGAATGA AGCAGAGAGG 
AAATCCGGCC TGGACCAGAT CAAGGGTGCT TTACAACTAG GCATGGTGGA TATTGCCCGG 
CAGACGGTGG AATTTCTATA TGAGGAGAAT GGCGGCATCC CAAGAGACCT GTATCTTCCC 
ACCATTGAGG ATATTAAAGA TGAAGCAAAC AAGTTCACAA TTGACAAAGT TCGAAAAGGT 
CTCACAGTTG TAACTCGCTC TCCAGACAGT AATAATGTAG CCAGCAGTAC CGTTGGAACT 
GCTTTGCCAA AATTTGCCAT CCGTGGGATG CTGAAAACCT TTGGACTTCA TGGAGTCGTC 
TTAGATGTCG ATTCAGTGAA CGAGCTGGTG CAGGTGGAAA CATATCTCCG GAGTGAGGGG 
GTGCTGGTGC GGTATTGGTA CCCCATTGAC ATGTTGGAAA GGCCCCCTGC AGGCTACCGA 
AGGACTGCCA CCAATGGGCT GGTCACACTG GACAACACCA ACCTTCAAAT TCACAGGGAG 
CTGCTGCGGT GTGAGGCTGC GTTGGCCAGG CTCTACTGCC GCATGGCCCT ACTCAACATC 
TTCGCCCCCA AGTTCCCTCA CTTGTTTACC CGCCTCTTCC ACATCCCTGC CATCCGAGAC 
ATCACTCTGG AGCACCTGCA ACTGCTGTCC AATCAGCTCC TCGCCCCTCC CCTCCCAGAT 
GGCACCATCA GCTCCAGCTC CATCCTCCTG GCGCAGTCCC TGCAGCACTG CATACACTCC 
CAGAACTGTT CCGCCACGGA CCTCTTTTAC CAGGGCAGCT CCCAGACAGT GAGAGAGTGG 
CTGAGTGTGG CCATCACCCG CACCCTGCAC CAGGGCGAGG AGAGCCTTCT GGAGCTCACG 
AGGCAGATCT GCTCCTTCCT GCAGACAGCA CCAGAGCAGT TCCCCTCCGA AGAATTCCCG 
ATATCCGAAT CCAAAGTCAA TATGGATGTT AATTTTCCCG GGGCGGCTTT TGTTGTGGTG 
TCTTGTAAAG AAAGTCAATC TGGATTCCGC AAAGACTCCT CTTTGTACAA GGCACCCTGG 
GCACGCGTCC TCGTGTACGG GCTTGGCCAC AAAGTGAAGC GAACCGGCCA GCTGAACCTC 
ATCGAGGCTG CCTGTTACCC ACGGGATGCG TCCCCGGCCA ACACTGGGCT TGCACCTCCG 
CCCACAGCTG ACCAGTACCC TTCTGTGGTG CTCTCCACAG ACAGGGTGCA CATCAAGCTA 
GGAGTGTCTC CCCCTCCTGG AGCCGTGCTG GTATTGCATT CTCTGCCCCT GGAGTTCCCG 
CTGGCTATGG CTTTCGCAGA GCAGCTGCTG TCATGGAAGT CGGAGGATGG CGAAGGGAAG 
TCTGAAGATG AGCCTGACAC TATTCCCACC TCAGTCCTCC TGCAGGTGGT GGAGCTGCTA 
GGAAATTTCC TGTGGACCAC AGACATGGCA GCCTGCGTGA AGGAACTTGT CTTCCACCTC 
CTGGCAGAGC TCCTGCGCAC GGTGCACGCA CTAGAGCAGA GGAAGCACCC CGCCGGCCTG 
TCCTCCTCCA TTGCCCTGCA GCTCAACCCC TGTCTGGCCA TGCTGATGGC CCTGCAGTCA 
GAGCTGCACA AGCTGTATGA CGAGGAGACC CAGAGCTGGG TCTCAGGCAG CACCTGTGGG 
GGCTCTGGGG CAGTGGCAAC CGGCGACCAG GGCAGGTTCT CCACGTACTT TCATGCACTC 
ATGGAAGGGT GCCTGGCAGT TGCCGAAGTG ACCCTGCCTA CCAACATGAG TGTCACAGCC 
AGTGGAGTGA CCTCAGCAAC CACCCCAAAC CTCAGTGACT CGTCGTCCTC CTCCTCTTCT 
TCTCCAGGAC AGACACCGCA GAGTCCCAGC CTCCTCTCCA AGAGGAAGAA AGTCAAGATG 
AAGCGGGAAA AGGCCTCCTC CTCTGGGAAG CGGCAGTCCT CCCGCACAGT GGAGTCCGAC 
TCCGCCGTGC TCAGCATCGG GGGCAGCAAG CCTGAGGACA TGCTCTGGTT CCACCGCGCG 
CTCACCTTGC TCATCATTCT GCGGCACCTC ACCAGGAAGG ACCCCCAGGG CCTGGGTGTG 
ACCAGCGATG CCGTTGCAGA TGCCTGCCAG GCCCTAGTGG GCCCCACTGC CCATAGCCGC 
CTGCTGGTGA TCTCTGGCAT CCCTACCCAC CTGGACGAGG GCATCGTCAG AGGTGCTATC 
CGCAAGGCCT GCAATGCCCA CGGCGGGGTC TTCAAAGATG AGATCTACAT TCCACTGCAG 
GAAGAAGACC CCAAGAAGCC CAAAGACAAG GCCGAAGGTG GCGATGGGAA GGCCGAGCCG 
GAGAAGACGC TGGGCTTCCC CAGTGCAGAC AGCCTGGAGG GGAGCACGTC CAGCAGCCTG 
GCCCCGGCCA TGAGCATCAG TGCCTCTGCC TCCACCAGCC AAGCCTCTGT CTGCAGCTCC 
CAGGGGGTCT CTCAGACCGT CAGCGACCTC TCGGTGGACC CACTGCCGTC CGGCCTGGAG 
TTGCCCATTC CTCCTGGCCT GCTGGAGCCC CACGTGGTGT CCAGCCAGGA GAGCCTGGAT 
ATTTCCCTGT GCAGCACTGG CAGCCTGGGC AGTCTGGGCA GCCTGGGGGA GCCTCTAGAC 
AATGCGGAGA CAGCCTCGGT GTCAGACATG GGCTCCATGT ACACAGTCAC TTCCCTGGAC 
AACCAGTCCC TTGCGGCACG CCCCATCAAA GGCTTTGCCG TCGTGGAGAT AAGATCCCGA 
GCCAAGATTG AGAAAATTCG AGCAAGTTTA TTTAACAATA ATGATTTAAT TGGCTTATCA 
AGTTTGGATG GTGAAGATGA ATTGATGGAA ATGTCCACAG AAGAGATTCT GACTGTATCT 
GTAGTGAACC AGAGTTTGTT TGATACTCAA GGGAGCCCGG GGTTAGAAGA TTATTTCAAT 
GATAAGTCAA TTAAAGGTGA GAAGCTGGTG CCTGGGGCCA GAGAGGTCCT GACAGAGATA 
TTTAAGAGCT GTGCCCACTC AGAGCAGATG CTGAGCCTGA CACCAGCAAA GCCCATCAGG 
GTCTCTGACA TATATCTCAG CAAGGAGCAG ATCAACTCCC AGACTCCAGG CAACCTCCTC 
CACATCTTCT TCACCAATGT CCGGCCTCCC AAGAAGGTGC TAGAAGACCA GCTCACCCAG 
ATCCTGAGGA AATATGGCGT GCCGAAGCCC AAGTTTGACA AGAGCAAGTA CAGCAAAGCT 
GGGAAGGAGC AGCACCCCGT GAAGGTGGTG AGCACCAAGC GGCCCATCAC CAAGCCGCCT 
GCCAAGGACA AAGCCGTGCT CAACAGTGTC AGCAGGACTG CCTTAAGTGA GAAGAAACCA 
ACTGTGAAGC CCAAGTCACC AGAAAAGAGC AAACCAGACG AAAAGGATCC AGAAAAGTCG 
CCCACAAAAA AACAAGAAGT CCCCGAGGAG AAGTACCTGA CCTTAGAAGG GTTTCACAAG 
TTTGTCATCG ACCGAGCCAA GCAGGACATC CGCAGCGTGT GGAGGGCAAT TCTGTCTTGT 
GGCTACGATC TTCATTTTGA GAGGTGTGCC TGCATTGATG TCCGACATGC GCAGAAGGCC 
TCAAGGAAGT GGACCCTGGA GATGGATGTA GCTCTCGTGC AGTACATCAA TCGGCTGTGC 
CGCCACCTTG CCATCACACC TGCAAGGCTC CACCCCCACG AGGTTTACCT GGACCCTGCC 
GATGCCACTG ACCCCAGGGT GGCCTGTCTC TTGAATGTGC CCATTGAGAG CCTGCGCCTG 
CGCTTCGCTT TGCTGCAGTC CCTCAACACC ACGCTGGAGA CCTTCTTCCT GCCCCTGGTG 
GAGCTGCGCC AGACACCCAT GTACACCCAC AGCATCGCGG CCCTTCTGAA GGAGGCCAAA 
GGGCTGATCT TCTATGACAC AAAGGTGACC GTGATGAACC GAGTGCTGAA TGCCACTGTG 
CAGAGGACAG CTGACCACGC GGCACCCGAG ATCACCTTGG ACCCATTGGA AATTGTGGGA 
GGGGAAATCA GAGCTTCTGA AAACTCCTAC TTCTGCCAGG CTGCCAGGCA ACTGGCCTCT 
GTGCCATCCT CTCAGCTCTG CGTCAAGCTG GCGAGTGGCG GCGACCCCAC CTACGCCTTC 
AACATCCGCT TCACCGGGGA GGAGGTCCAC GGCACCAGTG GCTCCTTCCG CCACTTCCTG 
TGGCAGGTGT GCAAGGAGCT GCAGAGCTCT TCGCTGTCGC TCCTGCTGCT GTGCCCCAGC 
TCTGCTGTCA ACAAGAACAA GGGCAAGTAC ATCCTGACGC CCAGCCCCAT CACCTACGGA 
GAGGAGCAGC TGCTGCACTT CCTAGGCCAG TTGCTGGGGA TCGCCATCCG GGCAGATGTC 
CCCCTTCCCC TGGACCTCCT GCCCTCCTTC TGGAAGACTC TGGTGGGGGA GCCCCTGGAC 
CCTGACCAGG ACCTGCAGGA AGCAGACATC CTTACCTATA ATTACGTCAA GAAGTTTGAG 
AGCATCAACG ACGAGAGCGA GCTGGAAGCC CTGTGCGCCG AGATTGCCTC CCAGCACCTG 
GCCACCGAGA GTCCTGACGG CCCCAGCAAG CCCTGCTGCA GGTTCACCTA CCTGACCATG 
ACGGGCGAGG AGGTGGAGCT GTGCAGCCGC GGCCGGCACA TCCCTGTGGC GTGGGAGAAC 
AAGGACATCT ACGCTGCAGC CATCCGCAGC CTGCGCTTGC GGGAGCTGCA GAACGTGGAA 
TGCGTGACAG CCGTGCGCGC AGGCCTGGGC TCTATCATCC CCCTGCAGCT GCTCACCACC 
CTCAGCCCCC TGGAGATGGA GCTGCGCACC TGTGGCCTCC CCTACATCAA CCTCGAGTTC 
CTGAAGGCCC ATACCATGTA CCAGGTGGGG CTAATGGAGA CGGACCAACA CATCGAGTTC 
TTCTGGGGGG CCCTGGAGAC GTTCACCCAG GAGGAGCTGT GCAGGTTCAT CAAGTTCGCC 
TGCAACCAGG AGCGCATCCC CTTCACCTGC CCCTGTAAAG ATGGGGGCCC CGACACAGCC 
CACGTACCCC CCTACCCCAT GAAGATCGCC CCCCCAGATG GCACAGCAGG TTCCCCAGAC 
TCCCGCTACA TCCGGGTGGA GACCTGCATG TTCATGATCA AGCTCCCCCA GTACTCCTCT 
TTGGAAATCA TGCTGGAGAA ACTGCGCTGT GCCATTCACT ACAGGGAAGA CCCCCTAAGT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589927.1
CDS	137..12982
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSLFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_021734252.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734252.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GAGCTAAAAG TCAGTATTTT GGCTCTTGCC 
ACCCAAATCC TGACTGGATG TGATGAAGTG TTGGAAATGC TACAGCAGGT CACCACTGCC 
CTCATAAACA GTGACATAGC AGATCGGGAG CAGAGGTTAA AAGGCTTGGA ACAAGTTACC 
AAGGCCACTA TGCTCGGTCA CCTTCTCCCA GTGTTACTGA CCTCCCTGAT GCACCCCAAT 
CTGCAGACCT TGACCATGGC CGACGCCTTG ATGCCTCAGC TGGTACAGCT GGTGCTCTAC 
ACCAGCCAGA CGGCTTTGCT GCTTAAAACT CAGTGTCCAG TGTTTGCTGA AGTGGGCTGC 
TTCCCATGTG GCACATCCGA CCAGAAGTGT AGGCTGTTCC CGGATGAGAG AATGCTGGAA 
GAAAAAGAGG AGCCAGGCTT CCTCACTGGC CTAAAGATTC CCGCCCCCTG GGCTGCTGGG 
AAAACCGTGG AAACAGTACA CCCTGTCAGA GACAACTACA AGTTTAAAGA AACTGTTCAT 
ATCCCAGGAG CTCGCTGCCT GTATCTGAGA TTTGATAGCA GATGCTCTTC ACAATATGAC 
TATGACAAAT CTAACAGTCT ATTTTCTGTT TTGAAGTTGG TGATATATGC GGGGCCTAAC 
ACAAACAGTA GGAAGGTTGC TGAATATGGA GGCAATACTC TGGGATATGG CAGCCGTAGT 
GTCTTAGGAA CTGGTTGGCC GAAGGACTTG GTGAAGGTGG AAGGAGATAC AGTCACCTTC 
TCCTTTGAAA TGAGAAGTGG CCGTGAACAC AACACTCCTG ATAAAGCTAT GTGGGGCTTT 
GCGTGCACAG TTCGCGCTCA GGAGTCCTCG GAGGACGTCT CAGGAGGCCT GCCTTTTCTG 
GTGGACCTGG CTTTAGGTCT GTCTGTGTTA GCTTGTTCCA TGTTAAGAAT CCTGTACAAT 
GGACCAGAAA TTACCAAAGA AGAGGAAGCC TGTCAGGAGC TTTTGCGGTC CAAACTTTTA 
CAAAGGTGCC AGTGGCAGGT GGAGGCCAAT GGCGTCATCT CGCCTGCCCT CACCCCAAGT 
CCCTCTCCAC TGCCTCTGAC CATAGAGGAA GACCGAGAGT TCACCTACCC CTCGGATGTC 
CTCGTGCCTC CTGTTGGGAA CTACTTTGAC CTGCCCCGGA TCCGGCTGCC TCCAGGAATC 
ATGATAAAGC TCAGGGAAAT TTCTGGGCGA GCTAGACCTC AATTTAGACC AAGTATAAAG 
GAAGTGATTC AGCCAGATGT GATGGAGGAA ATGGTGGTAT CATGTGTTAT TAAGCACTTG 
AACTTGGTCG ATGCACTGCA ATCCCTAATA AATTTCCAAT ATCAAGAAGA ACATGCTGAG 
GAATATGACT TACTATGTAA AATTATGGGA GAGACCTTTA AGAAACTCAA TGCCATGGAG 
AGACAGCTGC AGAGTGTTGC AGAACTGGAA CAGAAGTGGC AAAGTGAGGT TGATGATGCC 
ATGCAGGGGA AATTGGAGAA CAACATGCCT TTCTTTTATG ATTACCATTT TAATGAGAAC 
AAAATGAAAG AACTAGAACT TTTGTGTTCA ATGAAGGAAG TCTCCTTTGA TGGAAATGAT 
CTTGAAAACA TGGTCCTATC ACTGAGGGAG AAGTTCCTAC AAGAAGTGAA TTCTCTTATT 
CAGAAGCCCT CTCATCCACT GGCTAAAACG AAGACATTAG TGAAGAGTCT AATGAACCGT 
GCTGAGCTGC TGCTGCATGT CACCATTGCG GCCCAATCTG GCCTCACCAG GAGCATCTCG 
GGAACCCCTG CAGAGACACC GGCTTGTAAA TCAGCTTCTG AAACAAAGGT GATATCTCAT 
GCCGTCCGGC AGCCTGTTTT TCTCCGTAGT ATGTCGGCTC CTTCTGATCT AGAAATGATT 
GGTAATGAAG ATTTGGAATT TACTAGAGCA AGTCAGAGGC GTCGACATGT GACCAGCCAC 
CGGAGCAGCT CCTTTACACT CCTGCAATCG TTGGCCATTG AGGACAGCAG GGACAAGCCC 
ACCTATAGTG TCCTGCTGGG GCAGCTGTTT GCTTTCATTG GCACCAATCC CGACCAAGCT 
GTGTCCAGCA GCAGCTTCCT TCTTGCTGCA CAGACGAGGT GGCGGAGGGG AAACACACGC 
AAGCAGGCCC TGGTGCACAT GCGAGAGCTG CTCACAGCTG CCGTGCGCGT TGGGGGAGTG 
ACACATCTTG TGGGGCCAGT GACTATGGTC CTCCAGGGAG GACCCAGGAT TGAAGAGCTC 
ACCTGTGGTG GGATGGTTGA GCAGGTTCAG GAAGCCTTTG GCGAAACCAT GACTTCGGTT 
GTGTCTCTGT GTGCCCGTTA CCCTATCGCT TGTGCAAATA GCATTGGACT CTTGTGTACC 
ATACCTTATA CCAGGAGTGA GGAGAAGTGC CTGGTGCGCA GTGGCCTTGT CCAGCTCATG 
GATCGACTGT GCAGCTTGAG CAGTCAGACC GAGTCCAGCT CCAGTGAGAA ACAAACCAAG 
AAGCAGAAAG TGGCCACCAT GGCCTGGGCT GCCTTCCAGG TGCTGGCCAA CCGCTGTGTC 
GAGTGGGAAA AAGAGGAAGG TGGCTCCACA GAGGCTGTGC ACTCGGGGCT GGCTCGGCAG 
GTGTCCAGCC TTCTCACGAA TCATCTTGCC CGAGCCACTG AGTGCTGTGG CAACCAGGCT 
GCAGGAAATG ATGCTCTGCA GGATGTCCTG AGTCTGCTCA ACGACCTCTC GAGGAGCCAC 
ATAGGGAAAG CCATCCTGAG CCAGCCAGCT TGTGTGTCCA AGCTCCTCTC CCTGCTGCTT 
GACCAGCGCC CATCTCCAAA ATTGGTCCTT ATCATTCTTC AACTGTGCCG GGCGGCACTG 
CCATTGATGA GCGTAGAGGA CTGTGGAAAT GTGGAACTCC CGCCATGGAG CTATTCTGTC 
CCCTCCCTAA ACAGTGAGCA GGAGGATCCC AGTGACCCAG CTTCCAAGAT CGCCTCCTTG 
CTCTTAGCAA AGCTGGCAGA TTACGTGGTC CCAGGATGTC AGACAGTTCT TTCTCCAACT 
GCTTCTGAAC CGGATACCAC ATTAACAAAA ACCAGTCCCA AGAATTCTTT AAAAGGAGAT 
AAAGATCCTG GAGAAGAGAG TGAAGCAGTG GACGGCAAGC TCTCCATATT TATCCACAAA 
CGGGAAGACC AGTCCTCCCA CGAAGTTCTC CAACCATTAC TAAGTAGCTC AGAAGGGCGG 
CCCTTCCGAC TTGGTACTGG TGCCAACATG GAGAAAGTTG TGAAGATGGA TAGAGACATG 
ACCAAGGGTG GCTGTTGCGA AGTGATCACA GAAGAGGCTG CAGCCGCCCT TCGGAAAGCC 
ACCAAGTGGG CACAGTCAGG CCTCATCGTC AGCATTGGGC CACCAGTAGA GTCTGTCAAT 
CCAGAGACTG TGAGTGGACT ATCCACAGGT GACAAAAAGA AAACTGCCCA AACCTCCATC 
TGCAGAGAGA GGAACTCAGA ACTCGCCAGG ACTGACCCTG TCCGACCCTT CATCAGTGGG 
CATGTGGCAA ACAGCATGGC TGCAGAAGTG ATCGCTTTGC TTCATAGCTT GCTAATGGCA 
CCTGAATCTA ACGCGGCTCA AATATGGACC ACAACAGCAG AAAAGGTTCT GTCTCGTGCA 
TTGATGTACA TTCCACAACT GGGGAAACAT GCAGAAAGCA TTCTGGAAAA TGGCAGCAGC 
AGTGGCAGAA AACTTGCCAA ACTTCAGAGA ATTGCTCGCC AGGCTGTCGC TGCACTGTGT 
GCACTTGGAG GCTTCAAAGA GACAATCAAG ATAGGATCTG AGGTTCAGGT TTTAGGTAGA 
GGAATATCAG GAAGCATTGG AGTAGTGGCT TCTATCAACG AACAGGAAGG TATCGCTACA 
GTCAGATTTC CACCCATAGA CTGTCGAAAG ACTTCTCAAG CGTCAGACAC ATTGACTATT 
CCATTGTCTA GACTTTGTGT TCCAAGGTCA GAGGCACTGC CCCTCCACAA ACTGTCCATT 
ACTGAAAAGG TGGTACAGGC AGTTCAGTCC ATGCTGCTTC CACAGGAAGG CAGTCTCTCA 
ATTCACACCT CGCTTCCTGC AACAGGAGAT GGCTCTGCAC CTGTGATGGC AGTCGTCCGG 
CTCCTGGCTG AAATAAGAAC AAGAGCATGC CTGGTCATGG CCCAGCTTTT AGAAGACAGC 
TTATTTTGTG AAGAATTCAT ACAGCAGTGT CCAGCAGCTG TTGAAGTTCT TAATCTGGTA 
GCCCAGGAAT GCAGTGCTGG AGAGCGACTT GCTGTTGTGG AGGTGCAGTG TGAGCGGCTG 
AGGATGCTCT ATCGGGACTG CGCCCGGCCC CCGCCCCCTC CCCTGCAGGC TGACCGGAGA 
CAGCCCAAAG AGATAACTTG GAGCCCCTCC CGAGTGTTTC CTCCAGTGCG GGCCTGCATG 
TTCTCATCTC ACCTGACCTC GGTCACATTC CTGGCTGACC CCAGCGCTGG GGGAGGTCTG 
CCCCGGGGCA CCTTTATCTA CGCCACCTCA CCTCTGCCAG TTCAGGCCCC ATCTTTTTAC 
TGGGAAATTG AAATTGTTTC CTATGGAGAT ACTGATGACG ACACTGGACC AATAGTTTCC 
TTTGGCTTTG CTACAGAAGC CGAGAAACGA GATGGAGCTT GGACCAACCC AGTGGGCACT 
TGCCTTTTCC ACAACAATGG CCGAGCCGTG CACTACAACG GCTCCAGTCT GCTCCAGTGG 
AAGAGCGTTC GCTTAGACGT GACTCTCTCT CCTGGTGACG TGGCAGGAAT TGGCTGGGAG 
AGAACCGAGG GTACTCCACC TCCACCGGGG CAGCCAGCCA AGGGCCGGGT GTACTTCACA 
TATTGCGGGC AGCGCCTTTC ACCATACCTT GAGGATGTCT CTGGTGGGAT GTGGCCTGTG 
GTTCACATTC AGAAAAAGAA CACCAAAACT CGAGCCAACT TTGGCTCCCG CCCATTTGCC 
TATGCCGAGG GGCAGGCCCA TCGCAACGCA GCTGACCTAT GCACTGACCT GGCAGAGGAG 
ATTAGCGCAA ACTTCGAGGC CCTGCCCTTT GCCATGGCAT CTGACAGTGA CAACGATGCC 
GGCACCAGTA TTGCATCAGA CCCAGGCACT CATGGGCCAC CGTGCCGGAT TGCAGCCGTG 
GCCACCGCTC AGCAACAATA CGATAGTGAC ACCTCCTGTC ATTATAAAGT TGAGCTCAGC 
TATGAGAATT TCATCACCTC TGGCCCAGAC CCTCACCCGC CTCCCATTGC AGACGATGAG 
AGTGATGATG ATGACGATGA TGACATTCCA CAGGAGGACC ACTATGCCCT GCTGGTGAAA 
GCTTGGGAGA CCAAGGTTTT TCCCACCATC CGAAGACGCT TCCGGAATGA AGCAGAGAGG 
AAATCCGGCC TGGACCAGAT CAAGGGTGCT TTACAACTAG GCATGGTGGA TATTGCCCGG 
CAGACGGTGG AATTTCTATA TGAGGAGAAT GGCGGCATCC CAAGAGACCT GTATCTTCCC 
ACCATTGAGG ATATTAAAGA TGAAGCAAAC AAGTTCACAA TTGACAAAGT TCGAAAAGGT 
CTCACAGTTG TAACTCGCTC TCCAGACAGT AATAATGTAG CCAGCAGTAC CGTTGGAACT 
GCTTTGCCAA AATTTGCCAT CCGTGGGATG CTGAAAACCT TTGGACTTCA TGGAGTCGTC 
TTAGATGTCG ATTCAGTGAA CGAGCTGGTG CAGGTGGAAA CATATCTCCG GAGTGAGGGG 
GTGCTGGTGC GGTATTGGTA CCCCATTGAC ATGTTGGAAA GGCCCCCTGC AGGCTACCGA 
AGGACTGCCA CCAATGGGCT GGTCACACTG GACAACACCA ACCTTCAAAT TCACAGGGAG 
CTGCTGCGGT GTGAGGCTGC GTTGGCCAGG CTCTACTGCC GCATGGCCCT ACTCAACATC 
TTCGCCCCCA AGTTCCCTCA CTTGTTTACC CGCCTCTTCC ACATCCCTGC CATCCGAGAC 
ATCACTCTGG AGCACCTGCA ACTGCTGTCC AATCAGCTCC TCGCCCCTCC CCTCCCAGAT 
GGCACCATCA GCTCCAGCTC CATCCTCCTG GCGCAGTCCC TGCAGCACTG CATACACTCC 
CAGAACTGTT CCGCCACGGA CCTCTTTTAC CAGGGCAGCT CCCAGACAGT GAGAGAGTGG 
CTGAGTGTGG CCATCACCCG CACCCTGCAC CAGGGCGAGG AGAGCCTTCT GGAGCTCACG 
AGGCAGATCT GCTCCTTCCT GCAGACAGCA CCAGAGCAGT TCCCCTCCGA AGAATTCCCG 
ATATCCGAAT CCAAAGTCAA TATGGATGTT AATTTTCCCG GGGCGGCTTT TGTTGTGGTG 
TCTTGTAAAG AAAGTCAATC TGGATTCCGC AAAGACTCCT CTTTGTACAA GGCACCCTGG 
GCACGCGTCC TCGTGTACGG GCTTGGCCAC AAAGTGAAGC GAACCGGCCA GCTGAACCTC 
ATCGAGGCTG CCTGTTACCC ACGGGATGCG TCCCCGGCCA ACACTGGGCT TGCACCTCCG 
CCCACAGCTG ACCAGTACCC TTCTGTGGTG CTCTCCACAG ACAGGGTGCA CATCAAGCTA 
GGAGTGTCTC CCCCTCCTGG AGCCGTGCTG GTATTGCATT CTCTGCCCCT GGAGTTCCCG 
CTGGCTATGG CTTTCGCAGA GCAGCTGCTG TCATGGAAGT CGGAGGATGG CGAAGGGAAG 
TCTGAAGATG AGCCTGACAC TATTCCCACC TCAGTCCTCC TGCAGGTGGT GGAGCTGCTA 
GGAAATTTCC TGTGGACCAC AGACATGGCA GCCTGCGTGA AGGAACTTGT CTTCCACCTC 
CTGGCAGAGC TCCTGCGCAC GGTGCACGCA CTAGAGCAGA GGAAGCACCC CGCCGGCCTG 
TCCTCCTCCA TTGCCCTGCA GCTCAACCCC TGTCTGGCCA TGCTGATGGC CCTGCAGTCA 
GAGCTGCACA AGCTGTATGA CGAGGAGACC CAGAGCTGGG TCTCAGGCAG CACCTGTGGG 
GGCTCTGGGG CAGTGGCAAC CGGCGACCAG GGCAGGTTCT CCACGTACTT TCATGCACTC 
ATGGAAGGGT GCCTGGCAGT TGCCGAAGTG ACCCTGCCTA CCAACATGAG TGTCACAGCC 
AGTGGAGTGA CCTCAGCAAC CACCCCAAAC CTCAGTGACT CGTCGTCCTC CTCCTCTTCT 
TCTCCAGGAC AGACACCGCA GAGTCCCAGC CTCCTCTCCA AGAGGAAGAA AGTCAAGATG 
AAGCGGGAAA AGGCCTCCTC CTCTGGGAAG CGGCAGTCCT CCCGCACAGT GGAGTCCGAC 
TCCGCCGTGC TCAGCATCGG GGGCAGCAAG CCTGAGGACA TGCTCTGGTT CCACCGCGCG 
CTCACCTTGC TCATCATTCT GCGGCACCTC ACCAGGAAGG ACCCCCAGGG CCTGGGTGTG 
ACCAGCGATG CCGTTGCAGA TGCCTGCCAG GCCCTAGTGG GCCCCACTGC CCATAGCCGC 
CTGCTGGTGA TCTCTGGCAT CCCTACCCAC CTGGACGAGG GCATCGTCAG AGGTGCTATC 
CGCAAGGCCT GCAATGCCCA CGGCGGGGTC TTCAAAGATG AGATCTACAT TCCACTGCAG 
GAAGAAGACC CCAAGAAGCC CAAAGACAAG GCCGAAGGTG GCGATGGGAA GGCCGAGCCG 
GAGAAGACGC TGGGCTTCCC CAGTGCAGAC AGCCTGGAGG GGAGCACGTC CAGCAGCCTG 
GCCCCGGCCA TGAGCATCAG TGCCTCTGCC TCCACCAGCC AAGCCTCTGT CTGCAGCTCC 
CAGGGGGTCT CTCAGACCGT CAGCGACCTC TCGGTGGACC CACTGCCGTC CGGCCTGGAG 
TTGCCCATTC CTCCTGGCCT GCTGGAGCCC CACGTGGTGT CCAGCCAGGA GAGCCTGGAT 
ATTTCCCTGT GCAGCACTGG CAGCCTGGGC AGTCTGGGCA GCCTGGGGGA GCCTCTAGAC 
AATGCGGAGA CAGCCTCGGT GTCAGACATG GGCTCCATGT ACACAGTCAC TTCCCTGGAC 
AACCAGTCCC TTGCGGCACG CCCCATCAAA GGCTTTGCCG TCGTGGAGAT AAGATCCCGA 
GCCAAGATTG AGAAAATTCG AGCAAGTTTA TTTAACAATA ATGATTTAAT TGGCTTATCA 
AGTTTGGATG GTGAAGATGA ATTGATGGAA ATGTCCACAG AAGAGATTCT GACTGTATCT 
GTAGTGAACC AGAGTTTGTT TGATACTCAA GGGAGCCCGG GGTTAGAAGA TTATTTCAAT 
GATAAGTCAA TTAAAGGTGA GAAGCTGGTG CCTGGGGCCA GAGAGGTCCT GACAGAGATA 
TTTAAGAGCT GTGCCCACTC AGAGCAGATG CTGAGCCTGA CACCAGCAAA GCCCATCAGG 
GTCTCTGACA TATATCTCAG CAAGGAGCAG ATCAACTCCC AGACTCCAGG CAACCTCCTC 
CACATCTTCT TCACCAATGT CCGGCCTCCC AAGAAGGTGC TAGAAGACCA GCTCACCCAG 
ATCCTGAGGA AATATGGCGT GCCGAAGCCC AAGTTTGACA AGAGCAAGTA CAGCAAAGCT 
GGGAAGGAGC AGCACCCCGT GAAGGTGGTG AGCACCAAGC GGCCCATCAC CAAGCCGCCT 
GCCAAGGACA AAGCCGTGCT CAACAGTGTC AGCAGGACTG CCTTAAGTGA GAAGAAACCA 
ACTGTGAAGC CCAAGTCACC AGAAAAGAGC AAACCAGACG AAAAGGATCC AGAAAAGTCG 
CCCACAAAAA AACAAGAAGT CCCCGAGGAG AAGTACCTGA CCTTAGAAGG GTTTCACAAG 
TTTGTCATCG ACCGAGCCAA GCAGGACATC CGCAGCGTGT GGAGGGCAAT TCTGTCTTGT 
GGCTACGATC TTCATTTTGA GAGGTGTGCC TGCATTGATG TCCGACATGC GCAGAAGGCC 
TCAAGGAAGT GGACCCTGGA GATGGATGTA GCTCTCGTGC AGTACATCAA TCGGCTGTGC 
CGCCACCTTG CCATCACACC TGCAAGGCTC CACCCCCACG AGGTTTACCT GGACCCTGCC 
GATGCCACTG ACCCCAGGGT GGCCTGTCTC TTGAATGTGC CCATTGAGAG CCTGCGCCTG 
CGCTTCGCTT TGCTGCAGTC CCTCAACACC ACGCTGGAGA CCTTCTTCCT GCCCCTGGTG 
GAGCTGCGCC AGACACCCAT GTACACCCAC AGCATCGCGG CCCTTCTGAA GGAGGCCAAA 
GGGCTGATCT TCTATGACAC AAAGGTGACC GTGATGAACC GAGTGCTGAA TGCCACTGTG 
CAGAGGACAG CTGACCACGC GGCACCCGAG ATCACCTTGG ACCCATTGGA AATTGTGGGA 
GGGGAAATCA GAGCTTCTGA AAACTCCTAC TTCTGCCAGG CTGCCAGGCA ACTGGCCTCT 
GTGCCATCCT CTCAGCTCTG CGTCAAGCTG GCGAGTGGCG GCGACCCCAC CTACGCCTTC 
AACATCCGCT TCACCGGGGA GGAGGTCCAC GGCACCAGTG GCTCCTTCCG CCACTTCCTG 
TGGCAGGTGT GCAAGGAGCT GCAGAGCTCT TCGCTGTCGC TCCTGCTGCT GTGCCCCAGC 
TCTGCTGTCA ACAAGAACAA GGGCAAGTAC ATCCTGACGC CCAGCCCCAT CACCTACGGA 
GAGGAGCAGC TGCTGCACTT CCTAGGCCAG TTGCTGGGGA TCGCCATCCG GGCAGATGTC 
CCCCTTCCCC TGGACCTCCT GCCCTCCTTC TGGAAGACTC TGGTGGGGGA GCCCCTGGAC 
CCTGACCAGG ACCTGCAGGA AGCAGACATC CTTACCTATA ATTACGTCAA GAAGTTTGAG 
AGCATCAACG ACGAGAGCGA GCTGGAAGCC CTGTGCGCCG AGATTGCCTC CCAGCACCTG 
GCCACCGAGA GTCCTGACGG CCCCAGCAAG CCCTGCTGCA GGTTCACCTA CCTGACCATG 
ACGGGCGAGG AGGTGGAGCT GTGCAGCCGC GGCCGGCACA TCCCTGTGGC GTGGGAGAAC 
AAGGACATCT ACGCTGCAGC CATCCGCAGC CTGCGCTTGC GGGAGCTGCA GAACGTGGAA 
TGCGTGACAG CCGTGCGCGC AGGCCTGGGC TCTATCATCC CCCTGCAGCT GCTCACCACC 
CTCAGCCCCC TGGAGATGGA GCTGCGCACC TGTGGCCTCC CCTACATCAA CCTCGAGTTC 
CTGAAGGCCC ATACCATGTA CCAGGTGGGG CTAATGGAGA CGGACCAACA CATCGAGTTC 
TTCTGGGGGG CCCTGGAGAC GTTCACCCAG GAGGAGCTGT GCAGGTTCAT CAAGTTCGCC 
TGCAACCAGG AGCGCATCCC CTTCACCTGC CCCTGTAAAG ATGGGGGCCC CGACACAGCC 
CACGTACCCC CCTACCCCAT GAAGATCGCC CCCCCAGATG GCACAGCAGG TTCCCCAGAC 
TCCCGCTACA TCCGGGTGGA GACCTGCATG TTCATGATCA AGCTCCCCCA GTACTCCTCT 
TTGGAAATCA TGCTGGAGAA ACTGCGCTGT GCCATTCACT ACAGGGAAGA CCCCCTAAGT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734253.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589928.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X4, mRNA.

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CloneID	OIc83556
Clone ID Related Accession (Same CDS sequence)	XM_021734253.1
Accession Version	XM_021734253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATTGGT GATATATGCG GGGCCTAACA CAAACAGTAG GAAGGTTGCT 
GAATATGGAG GCAATACTCT GGGATATGGC AGCCGTAGTG TCTTAGGAAC TGGTTGGCCG 
AAGGACTTGG TGAAGGTGGA AGGAGATACA GTCACCTTCT CCTTTGAAAT GAGAAGTGGC 
CGTGAACACA ACACTCCTGA TAAAGCTATG TGGGGCTTTG CGTGCACAGT TCGCGCTCAG 
GAGTCCTCGG AGGACGTCTC AGGAGGCCTG CCTTTTCTGG TGGACCTGGC TTTAGGTCTG 
TCTGTGTTAG CTTGTTCCAT GTTAAGAATC CTGTACAATG GACCAGAAAT TACCAAAGAA 
GAGGAAGCCT GTCAGGAGCT TTTGCGGTCC AAACTTTTAC AAAGGTGCCA GTGGCAGGTG 
GAGGCCAATG GCGTCATCTC GCCTGCCCTC ACCCCAAGTC CCTCTCCACT GCCTCTGACC 
ATAGAGGAAG ACCGAGAGTT CACCTACCCC TCGGATGTCC TCGTGCCTCC TGTTGGGAAC 
TACTTTGACC TGCCCCGGAT CCGGCTGCCT CCAGGAATCA TGATAAAGCT CAGGGAAATT 
TCTGGGCGAG CTAGACCTCA ATTTAGACCA AGTATAAAGG AAGTGATTCA GCCAGATGTG 
ATGGAGGAAA TGGTGGTATC ATGTGTTATT AAGCACTTGA ACTTGGTCGA TGCACTGCAA 
TCCCTAATAA ATTTCCAATA TCAAGAAGAA CATGCTGAGG AATATGACTT ACTATGTAAA 
ATTATGGGAG AGACCTTTAA GAAACTCAAT GCCATGGAGA GACAGCTGCA GAGTGTTGCA 
GAACTGGAAC AGAAGTGGCA AAGTGAGGTT GATGATGCCA TGCAGGGGAA ATTGGAGAAC 
AACATGCCTT TCTTTTATGA TTACCATTTT AATGAGAACA AAATGAAAGA ACTAGAACTT 
TTGTGTTCAA TGAAGGAAGT CTCCTTTGAT GGAAATGATC TTGAAAACAT GGTCCTATCA 
CTGAGGGAGA AGTTCCTACA AGAAGTGAAT TCTCTTATTC AGAAGCCCTC TCATCCACTG 
GCTAAAACGA AGACATTAGT GAAGAGTCTA ATGAACCGTG CTGAGCTGCT GCTGCATGTC 
ACCATTGCGG CCCAATCTGG CCTCACCAGG AGCATCTCGG GAACCCCTGC AGAGACACCG 
GCTTGTAAAT CAGCTTCTGA AACAAAGGTG ATATCTCATG CCGTCCGGCA GCCTGTTTTT 
CTCCGTAGTA TGTCGGCTCC TTCTGATCTA GAAATGATTG GTAATGAAGA TTTGGAATTT 
ACTAGAGCAA GTCAGAGGCG TCGACATGTG ACCAGCCACC GGAGCAGCTC CTTTACACTC 
CTGCAATCGT TGGCCATTGA GGACAGCAGG GACAAGCCCA CCTATAGTGT CCTGCTGGGG 
CAGCTGTTTG CTTTCATTGG CACCAATCCC GACCAAGCTG TGTCCAGCAG CAGCTTCCTT 
CTTGCTGCAC AGACGAGGTG GCGGAGGGGA AACACACGCA AGCAGGCCCT GGTGCACATG 
CGAGAGCTGC TCACAGCTGC CGTGCGCGTT GGGGGAGTGA CACATCTTGT GGGGCCAGTG 
ACTATGGTCC TCCAGGGAGG ACCCAGGATT GAAGAGCTCA CCTGTGGTGG GATGGTTGAG 
CAGGTTCAGG AAGCCTTTGG CGAAACCATG ACTTCGGTTG TGTCTCTGTG TGCCCGTTAC 
CCTATCGCTT GTGCAAATAG CATTGGACTC TTGTGTACCA TACCTTATAC CAGGAGTGAG 
GAGAAGTGCC TGGTGCGCAG TGGCCTTGTC CAGCTCATGG ATCGACTGTG CAGCTTGAGC 
AGTCAGACCG AGTCCAGCTC CAGTGAGAAA CAAACCAAGA AGCAGAAAGT GGCCACCATG 
GCCTGGGCTG CCTTCCAGGT GCTGGCCAAC CGCTGTGTCG AGTGGGAAAA AGAGGAAGGT 
GGCTCCACAG AGGCTGTGCA CTCGGGGCTG GCTCGGCAGG TGTCCAGCCT TCTCACGAAT 
CATCTTGCCC GAGCCACTGA GTGCTGTGGC AACCAGGCTG CAGGAAATGA TGCTCTGCAG 
GATGTCCTGA GTCTGCTCAA CGACCTCTCG AGGAGCCACA TAGGGAAAGC CATCCTGAGC 
CAGCCAGCTT GTGTGTCCAA GCTCCTCTCC CTGCTGCTTG ACCAGCGCCC ATCTCCAAAA 
TTGGTCCTTA TCATTCTTCA ACTGTGCCGG GCGGCACTGC CATTGATGAG CGTAGAGGAC 
TGTGGAAATG TGGAACTCCC GCCATGGAGC TATTCTGTCC CCTCCCTAAA CAGTGAGCAG 
GAGGATCCCA GTGACCCAGC TTCCAAGATC GCCTCCTTGC TCTTAGCAAA GCTGGCAGAT 
TACGTGGTCC CAGGATGTCA GACAGTTCTT TCTCCAACTG CTTCTGAACC GGATACCACA 
TTAACAAAAA CCAGTCCCAA GAATTCTTTA AAAGGAGATA AAGATCCTGG AGAAGAGAGT 
GAAGCAGTGG ACGGCAAGCT CTCCATATTT ATCCACAAAC GGGAAGACCA GTCCTCCCAC 
GAAGTTCTCC AACCATTACT AAGTAGCTCA GAAGGGCGGC CCTTCCGACT TGGTACTGGT 
GCCAACATGG AGAAAGTTGT GAAGATGGAT AGAGACATGA CCAAGGGTGG CTGTTGCGAA 
GTGATCACAG AAGAGGCTGC AGCCGCCCTT CGGAAAGCCA CCAAGTGGGC ACAGTCAGGC 
CTCATCGTCA GCATTGGGCC ACCAGTAGAG TCTGTCAATC CAGAGACTGT GAGTGGACTA 
TCCACAGGTG ACAAAAAGAA AACTGCCCAA ACCTCCATCT GCAGAGAGAG GAACTCAGAA 
CTCGCCAGGA CTGACCCTGT CCGACCCTTC ATCAGTGGGC ATGTGGCAAA CAGCATGGCT 
GCAGAAGTGA TCGCTTTGCT TCATAGCTTG CTAATGGCAC CTGAATCTAA CGCGGCTCAA 
ATATGGACCA CAACAGCAGA AAAGGTTCTG TCTCGTGCAT TGATGTACAT TCCACAACTG 
GGGAAACATG CAGAAAGCAT TCTGGAAAAT GGCAGCAGCA GTGGCAGAAA ACTTGCCAAA 
CTTCAGAGAA TTGCTCGCCA GGCTGTCGCT GCACTGTGTG CACTTGGAGG CTTCAAAGAG 
ACAATCAAGA TAGGATCTGA GGTTCAGGTT TTAGGTAGAG GAATATCAGG AAGCATTGGA 
GTAGTGGCTT CTATCAACGA ACAGGAAGGT ATCGCTACAG TCAGATTTCC ACCCATAGAC 
TGTCGAAAGA CTTCTCAAGC GTCAGACACA TTGACTATTC CATTGTCTAG ACTTTGTGTT 
CCAAGGTCAG AGGCACTGCC CCTCCACAAA CTGTCCATTA CTGAAAAGGT GGTACAGGCA 
GTTCAGTCCA TGCTGCTTCC ACAGGAAGGC AGTCTCTCAA TTCACACCTC GCTTCCTGCA 
ACAGGAGATG GCTCTGCACC TGTGATGGCA GTCGTCCGGC TCCTGGCTGA AATAAGAACA 
AGAGCATGCC TGGTCATGGC CCAGCTTTTA GAAGACAGCT TATTTTGTGA AGAATTCATA 
CAGCAGTGTC CAGCAGCTGT TGAAGTTCTT AATCTGGTAG CCCAGGAATG CAGTGCTGGA 
GAGCGACTTG CTGTTGTGGA GGTGCAGTGT GAGCGGCTGA GGATGCTCTA TCGGGACTGC 
GCCCGGCCCC CGCCCCCTCC CCTGCAGGCT GACCGGAGAC AGCCCAAAGA GATAACTTGG 
AGCCCCTCCC GAGTGTTTCC TCCAGTGCGG GCCTGCATGT TCTCATCTCA CCTGACCTCG 
GTCACATTCC TGGCTGACCC CAGCGCTGGG GGAGGTCTGC CCCGGGGCAC CTTTATCTAC 
GCCACCTCAC CTCTGCCAGT TCAGGCCCCA TCTTTTTACT GGGAAATTGA AATTGTTTCC 
TATGGAGATA CTGATGACGA CACTGGACCA ATAGTTTCCT TTGGCTTTGC TACAGAAGCC 
GAGAAACGAG ATGGAGCTTG GACCAACCCA GTGGGCACTT GCCTTTTCCA CAACAATGGC 
CGAGCCGTGC ACTACAACGG CTCCAGTCTG CTCCAGTGGA AGAGCGTTCG CTTAGACGTG 
ACTCTCTCTC CTGGTGACGT GGCAGGAATT GGCTGGGAGA GAACCGAGGG TACTCCACCT 
CCACCGGGGC AGCCAGCCAA GGGCCGGGTG TACTTCACAT ATTGCGGGCA GCGCCTTTCA 
CCATACCTTG AGGATGTCTC TGGTGGGATG TGGCCTGTGG TTCACATTCA GAAAAAGAAC 
ACCAAAACTC GAGCCAACTT TGGCTCCCGC CCATTTGCCT ATGCCGAGGG GCAGGCCCAT 
CGCAACGCAG CTGACCTATG CACTGACCTG GCAGAGGAGA TTAGCGCAAA CTTCGAGGCC 
CTGCCCTTTG CCATGGCATC TGACAGTGAC AACGATGCCG GCACCAGTAT TGCATCAGAC 
CCAGGCACTC ATGGGCCACC GTGCCGGATT GCAGCCGTGG CCACCGCTCA GCAACAATAC 
GATAGTGACA CCTCCTGTCA TTATAAAGTT GAGCTCAGCT ATGAGAATTT CATCACCTCT 
GGCCCAGACC CTCACCCGCC TCCCATTGCA GACGATGAGA GTGATGATGA TGACGATGAT 
GACATTCCAC AGGAGGACCA CTATGCCCTG CTGGTGAAAG CTTGGGAGAC CAAGGTTTTT 
CCCACCATCC GAAGACGCTT CCGGAATGAA GCAGAGAGGA AATCCGGCCT GGACCAGATC 
AAGGGTGCTT TACAACTAGG CATGGTGGAT ATTGCCCGGC AGACGGTGGA ATTTCTATAT 
GAGGAGAATG GCGGCATCCC AAGAGACCTG TATCTTCCCA CCATTGAGGA TATTAAAGAT 
GAAGCAAACA AGTTCACAAT TGACAAAGTT CGAAAAGGTC TCACAGTTGT AACTCGCTCT 
CCAGACAGTA ATAATGTAGC CAGCAGTACC GTTGGAACTG CTTTGCCAAA ATTTGCCATC 
CGTGGGATGC TGAAAACCTT TGGACTTCAT GGAGTCGTCT TAGATGTCGA TTCAGTGAAC 
GAGCTGGTGC AGGTGGAAAC ATATCTCCGG AGTGAGGGGG TGCTGGTGCG GTATTGGTAC 
CCCATTGACA TGTTGGAAAG GCCCCCTGCA GGCTACCGAA GGACTGCCAC CAATGGGCTG 
GTCACACTGG ACAACACCAA CCTTCAAATT CACAGGGAGC TGCTGCGGTG TGAGGCTGCG 
TTGGCCAGGC TCTACTGCCG CATGGCCCTA CTCAACATCT TCGCCCCCAA GTTCCCTCAC 
TTGTTTACCC GCCTCTTCCA CATCCCTGCC ATCCGAGACA TCACTCTGGA GCACCTGCAA 
CTGCTGTCCA ATCAGCTCCT CGCCCCTCCC CTCCCAGATG GCACCATCAG CTCCAGCTCC 
ATCCTCCTGG CGCAGTCCCT GCAGCACTGC ATACACTCCC AGAACTGTTC CGCCACGGAC 
CTCTTTTACC AGGGCAGCTC CCAGACAGTG AGAGAGTGGC TGAGTGTGGC CATCACCCGC 
ACCCTGCACC AGGGCGAGGA GAGCCTTCTG GAGCTCACGA GGCAGATCTG CTCCTTCCTG 
CAGACAGCAC CAGAGCAGTT CCCCTCCGAA GAATTCCCGA TATCCGAATC CAAAGTCAAT 
ATGGATGTTA ATTTTCCCGG GGCGGCTTTT GTTGTGGTGT CTTGTAAAGA AAGTCAATCT 
GGATTCCGCA AAGACTCCTC TTTGTACAAG GCACCCTGGG CACGCGTCCT CGTGTACGGG 
CTTGGCCACA AAGTGAAGCG AACCGGCCAG CTGAACCTCA TCGAGGCTGC CTGTTACCCA 
CGGGATGCGT CCCCGGCCAA CACTGGGCTT GCACCTCCGC CCACAGCTGA CCAGTACCCT 
TCTGTGGTGC TCTCCACAGA CAGGGTGCAC ATCAAGCTAG GAGTGTCTCC CCCTCCTGGA 
GCCGTGCTGG TATTGCATTC TCTGCCCCTG GAGTTCCCGC TGGCTATGGC TTTCGCAGAG 
CAGCTGCTGT CATGGAAGTC GGAGGATGGC GAAGGGAAGT CTGAAGATGA GCCTGACACT 
ATTCCCACCT CAGTCCTCCT GCAGGTGGTG GAGCTGCTAG GAAATTTCCT GTGGACCACA 
GACATGGCAG CCTGCGTGAA GGAACTTGTC TTCCACCTCC TGGCAGAGCT CCTGCGCACG 
GTGCACGCAC TAGAGCAGAG GAAGCACCCC GCCGGCCTGT CCTCCTCCAT TGCCCTGCAG 
CTCAACCCCT GTCTGGCCAT GCTGATGGCC CTGCAGTCAG AGCTGCACAA GCTGTATGAC 
GAGGAGACCC AGAGCTGGGT CTCAGGCAGC ACCTGTGGGG GCTCTGGGGC AGTGGCAACC 
GGCGACCAGG GCAGGTTCTC CACGTACTTT CATGCACTCA TGGAAGGGTG CCTGGCAGTT 
GCCGAAGTGA CCCTGCCTAC CAACATGAGT GTCACAGCCA GTGGAGTGAC CTCAGCAACC 
ACCCCAAACC TCAGTGACTC GTCGTCCTCC TCCTCTTCTT CTCCAGGACA GACACCGCAG 
AGTCCCAGCC TCCTCTCCAA GAGGAAGAAA GTCAAGATGA AGCGGGAAAA GGCCTCCTCC 
TCTGGGAAGC GGCAGTCCTC CCGCACAGTG GAGTCCGACT CCGCCGTGCT CAGCATCGGG 
GGCAGCAAGC CTGAGGACAT GCTCTGGTTC CACCGCGCGC TCACCTTGCT CATCATTCTG 
CGGCACCTCA CCAGGAAGGA CCCCCAGGGC CTGGGTGTGA CCAGCGATGC CGTTGCAGAT 
GCCTGCCAGG CCCTAGTGGG CCCCACTGCC CATAGCCGCC TGCTGGTGAT CTCTGGCATC 
CCTACCCACC TGGACGAGGG CATCGTCAGA GGTGCTATCC GCAAGGCCTG CAATGCCCAC 
GGCGGGGTCT TCAAAGATGA GATCTACATT CCACTGCAGG AAGAAGACCC CAAGAAGCCC 
AAAGACAAGG CCGAAGGTGG CGATGGGAAG GCCGAGCCGG AGAAGACGCT GGGCTTCCCC 
AGTGCAGACA GCCTGGAGGG GAGCACGTCC AGCAGCCTGG CCCCGGCCAT GAGCATCAGT 
GCCTCTGCCT CCACCAGCCA AGCCTCTGTC TGCAGCTCCC AGGGGGTCTC TCAGACCGTC 
AGCGACCTCT CGGTGGACCC ACTGCCGTCC GGCCTGGAGT TGCCCATTCC TCCTGGCCTG 
CTGGAGCCCC ACGTGGTGTC CAGCCAGGAG AGCCTGGATA TTTCCCTGTG CAGCACTGGC 
AGCCTGGGCA GTCTGGGCAG CCTGGGGGAG CCTCTAGACA ATGCGGAGAC AGCCTCGGTG 
TCAGACATGG GCTCCATGTA CACAGTCACT TCCCTGGACA ACCAGTCCCT TGCGGCACGC 
CCCATCAAAG GCTTTGCCGT CGTGGAGATA AGATCCCGAG CCAAGATTGA GAAAATTCGA 
GCAAGTTTAT TTAACAATAA TGATTTAATT GGCTTATCAA GTTTGGATGG TGAAGATGAA 
TTGATGGAAA TGTCCACAGA AGAGATTCTG ACTGTATCTG TAGTGAACCA GAGTTTGTTT 
GATACTCAAG GGAGCCCGGG GTTAGAAGAT TATTTCAATG ATAAGTCAAT TAAAGGTGAG 
AAGCTGGTGC CTGGGGCCAG AGAGGTCCTG ACAGAGATAT TTAAGAGCTG TGCCCACTCA 
GAGCAGATGC TGAGCCTGAC ACCAGCAAAG CCCATCAGGG TCTCTGACAT ATATCTCAGC 
AAGGAGCAGA TCAACTCCCA GACTCCAGGC AACCTCCTCC ACATCTTCTT CACCAATGTC 
CGGCCTCCCA AGAAGGTGCT AGAAGACCAG CTCACCCAGA TCCTGAGGAA ATATGGCGTG 
CCGAAGCCCA AGTTTGACAA GAGCAAGTAC AGCAAAGCTG GGAAGGAGCA GCACCCCGTG 
AAGGTGGTGA GCACCAAGCG GCCCATCACC AAGCCGCCTG CCAAGGACAA AGCCGTGCTC 
AACAGTGTCA GCAGGACTGC CTTAAGTGAG AAGAAACCAA CTGTGAAGCC CAAGTCACCA 
GAAAAGAGCA AACCAGACGA AAAGGATCCA GAAAAGTCGC CCACAAAAAA ACAAGAAGTC 
CCCGAGGAGA AGTACCTGAC CTTAGAAGGG TTTCACAAGT TTGTCATCGA CCGAGCCAAG 
CAGGACATCC GCAGCGTGTG GAGGGCAATT CTGTCTTGTG GCTACGATCT TCATTTTGAG 
AGGTGTGCCT GCATTGATGT CCGACATGCG CAGAAGGCCT CAAGGAAGTG GACCCTGGAG 
ATGGATGTAG CTCTCGTGCA GTACATCAAT CGGCTGTGCC GCCACCTTGC CATCACACCT 
GCAAGGCTCC ACCCCCACGA GGTTTACCTG GACCCTGCCG ATGCCACTGA CCCCAGGGTG 
GCCTGTCTCT TGAATGTGCC CATTGAGAGC CTGCGCCTGC GCTTCGCTTT GCTGCAGTCC 
CTCAACACCA CGCTGGAGAC CTTCTTCCTG CCCCTGGTGG AGCTGCGCCA GACACCCATG 
TACACCCACA GCATCGCGGC CCTTCTGAAG GAGGCCAAAG GGCTGATCTT CTATGACACA 
AAGGTGACCG TGATGAACCG AGTGCTGAAT GCCACTGTGC AGAGGACAGC TGACCACGCG 
GCACCCGAGA TCACCTTGGA CCCATTGGAA ATTGTGGGAG GGGAAATCAG AGCTTCTGAA 
AACTCCTACT TCTGCCAGGC TGCCAGGCAA CTGGCCTCTG TGCCATCCTC TCAGCTCTGC 
GTCAAGCTGG CGAGTGGCGG CGACCCCACC TACGCCTTCA ACATCCGCTT CACCGGGGAG 
GAGGTCCACG GCACCAGTGG CTCCTTCCGC CACTTCCTGT GGCAGGTGTG CAAGGAGCTG 
CAGAGCTCTT CGCTGTCGCT CCTGCTGCTG TGCCCCAGCT CTGCTGTCAA CAAGAACAAG 
GGCAAGTACA TCCTGACGCC CAGCCCCATC ACCTACGGAG AGGAGCAGCT GCTGCACTTC 
CTAGGCCAGT TGCTGGGGAT CGCCATCCGG GCAGATGTCC CCCTTCCCCT GGACCTCCTG 
CCCTCCTTCT GGAAGACTCT GGTGGGGGAG CCCCTGGACC CTGACCAGGA CCTGCAGGAA 
GCAGACATCC TTACCTATAA TTACGTCAAG AAGTTTGAGA GCATCAACGA CGAGAGCGAG 
CTGGAAGCCC TGTGCGCCGA GATTGCCTCC CAGCACCTGG CCACCGAGAG TCCTGACGGC 
CCCAGCAAGC CCTGCTGCAG GTTCACCTAC CTGACCATGA CGGGCGAGGA GGTGGAGCTG 
TGCAGCCGCG GCCGGCACAT CCCTGTGGCG TGGGAGAACA AGGACATCTA CGCTGCAGCC 
ATCCGCAGCC TGCGCTTGCG GGAGCTGCAG AACGTGGAAT GCGTGACAGC CGTGCGCGCA 
GGCCTGGGCT CTATCATCCC CCTGCAGCTG CTCACCACCC TCAGCCCCCT GGAGATGGAG 
CTGCGCACCT GTGGCCTCCC CTACATCAAC CTCGAGTTCC TGAAGGCCCA TACCATGTAC 
CAGGTGGGGC TAATGGAGAC GGACCAACAC ATCGAGTTCT TCTGGGGGGC CCTGGAGACG 
TTCACCCAGG AGGAGCTGTG CAGGTTCATC AAGTTCGCCT GCAACCAGGA GCGCATCCCC 
TTCACCTGCC CCTGTAAAGA TGGGGGCCCC GACACAGCCC ACGTACCCCC CTACCCCATG 
AAGATCGCCC CCCCAGATGG CACAGCAGGT TCCCCAGACT CCCGCTACAT CCGGGTGGAG 
ACCTGCATGT TCATGATCAA GCTCCCCCAG TACTCCTCTT TGGAAATCAT GCTGGAGAAA 
CTGCGCTGTG CCATTCACTA CAGGGAAGAC CCCCTAAGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589928.1
CDS	137..12961
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_021734253.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734253.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATTGGT GATATATGCG GGGCCTAACA CAAACAGTAG GAAGGTTGCT 
GAATATGGAG GCAATACTCT GGGATATGGC AGCCGTAGTG TCTTAGGAAC TGGTTGGCCG 
AAGGACTTGG TGAAGGTGGA AGGAGATACA GTCACCTTCT CCTTTGAAAT GAGAAGTGGC 
CGTGAACACA ACACTCCTGA TAAAGCTATG TGGGGCTTTG CGTGCACAGT TCGCGCTCAG 
GAGTCCTCGG AGGACGTCTC AGGAGGCCTG CCTTTTCTGG TGGACCTGGC TTTAGGTCTG 
TCTGTGTTAG CTTGTTCCAT GTTAAGAATC CTGTACAATG GACCAGAAAT TACCAAAGAA 
GAGGAAGCCT GTCAGGAGCT TTTGCGGTCC AAACTTTTAC AAAGGTGCCA GTGGCAGGTG 
GAGGCCAATG GCGTCATCTC GCCTGCCCTC ACCCCAAGTC CCTCTCCACT GCCTCTGACC 
ATAGAGGAAG ACCGAGAGTT CACCTACCCC TCGGATGTCC TCGTGCCTCC TGTTGGGAAC 
TACTTTGACC TGCCCCGGAT CCGGCTGCCT CCAGGAATCA TGATAAAGCT CAGGGAAATT 
TCTGGGCGAG CTAGACCTCA ATTTAGACCA AGTATAAAGG AAGTGATTCA GCCAGATGTG 
ATGGAGGAAA TGGTGGTATC ATGTGTTATT AAGCACTTGA ACTTGGTCGA TGCACTGCAA 
TCCCTAATAA ATTTCCAATA TCAAGAAGAA CATGCTGAGG AATATGACTT ACTATGTAAA 
ATTATGGGAG AGACCTTTAA GAAACTCAAT GCCATGGAGA GACAGCTGCA GAGTGTTGCA 
GAACTGGAAC AGAAGTGGCA AAGTGAGGTT GATGATGCCA TGCAGGGGAA ATTGGAGAAC 
AACATGCCTT TCTTTTATGA TTACCATTTT AATGAGAACA AAATGAAAGA ACTAGAACTT 
TTGTGTTCAA TGAAGGAAGT CTCCTTTGAT GGAAATGATC TTGAAAACAT GGTCCTATCA 
CTGAGGGAGA AGTTCCTACA AGAAGTGAAT TCTCTTATTC AGAAGCCCTC TCATCCACTG 
GCTAAAACGA AGACATTAGT GAAGAGTCTA ATGAACCGTG CTGAGCTGCT GCTGCATGTC 
ACCATTGCGG CCCAATCTGG CCTCACCAGG AGCATCTCGG GAACCCCTGC AGAGACACCG 
GCTTGTAAAT CAGCTTCTGA AACAAAGGTG ATATCTCATG CCGTCCGGCA GCCTGTTTTT 
CTCCGTAGTA TGTCGGCTCC TTCTGATCTA GAAATGATTG GTAATGAAGA TTTGGAATTT 
ACTAGAGCAA GTCAGAGGCG TCGACATGTG ACCAGCCACC GGAGCAGCTC CTTTACACTC 
CTGCAATCGT TGGCCATTGA GGACAGCAGG GACAAGCCCA CCTATAGTGT CCTGCTGGGG 
CAGCTGTTTG CTTTCATTGG CACCAATCCC GACCAAGCTG TGTCCAGCAG CAGCTTCCTT 
CTTGCTGCAC AGACGAGGTG GCGGAGGGGA AACACACGCA AGCAGGCCCT GGTGCACATG 
CGAGAGCTGC TCACAGCTGC CGTGCGCGTT GGGGGAGTGA CACATCTTGT GGGGCCAGTG 
ACTATGGTCC TCCAGGGAGG ACCCAGGATT GAAGAGCTCA CCTGTGGTGG GATGGTTGAG 
CAGGTTCAGG AAGCCTTTGG CGAAACCATG ACTTCGGTTG TGTCTCTGTG TGCCCGTTAC 
CCTATCGCTT GTGCAAATAG CATTGGACTC TTGTGTACCA TACCTTATAC CAGGAGTGAG 
GAGAAGTGCC TGGTGCGCAG TGGCCTTGTC CAGCTCATGG ATCGACTGTG CAGCTTGAGC 
AGTCAGACCG AGTCCAGCTC CAGTGAGAAA CAAACCAAGA AGCAGAAAGT GGCCACCATG 
GCCTGGGCTG CCTTCCAGGT GCTGGCCAAC CGCTGTGTCG AGTGGGAAAA AGAGGAAGGT 
GGCTCCACAG AGGCTGTGCA CTCGGGGCTG GCTCGGCAGG TGTCCAGCCT TCTCACGAAT 
CATCTTGCCC GAGCCACTGA GTGCTGTGGC AACCAGGCTG CAGGAAATGA TGCTCTGCAG 
GATGTCCTGA GTCTGCTCAA CGACCTCTCG AGGAGCCACA TAGGGAAAGC CATCCTGAGC 
CAGCCAGCTT GTGTGTCCAA GCTCCTCTCC CTGCTGCTTG ACCAGCGCCC ATCTCCAAAA 
TTGGTCCTTA TCATTCTTCA ACTGTGCCGG GCGGCACTGC CATTGATGAG CGTAGAGGAC 
TGTGGAAATG TGGAACTCCC GCCATGGAGC TATTCTGTCC CCTCCCTAAA CAGTGAGCAG 
GAGGATCCCA GTGACCCAGC TTCCAAGATC GCCTCCTTGC TCTTAGCAAA GCTGGCAGAT 
TACGTGGTCC CAGGATGTCA GACAGTTCTT TCTCCAACTG CTTCTGAACC GGATACCACA 
TTAACAAAAA CCAGTCCCAA GAATTCTTTA AAAGGAGATA AAGATCCTGG AGAAGAGAGT 
GAAGCAGTGG ACGGCAAGCT CTCCATATTT ATCCACAAAC GGGAAGACCA GTCCTCCCAC 
GAAGTTCTCC AACCATTACT AAGTAGCTCA GAAGGGCGGC CCTTCCGACT TGGTACTGGT 
GCCAACATGG AGAAAGTTGT GAAGATGGAT AGAGACATGA CCAAGGGTGG CTGTTGCGAA 
GTGATCACAG AAGAGGCTGC AGCCGCCCTT CGGAAAGCCA CCAAGTGGGC ACAGTCAGGC 
CTCATCGTCA GCATTGGGCC ACCAGTAGAG TCTGTCAATC CAGAGACTGT GAGTGGACTA 
TCCACAGGTG ACAAAAAGAA AACTGCCCAA ACCTCCATCT GCAGAGAGAG GAACTCAGAA 
CTCGCCAGGA CTGACCCTGT CCGACCCTTC ATCAGTGGGC ATGTGGCAAA CAGCATGGCT 
GCAGAAGTGA TCGCTTTGCT TCATAGCTTG CTAATGGCAC CTGAATCTAA CGCGGCTCAA 
ATATGGACCA CAACAGCAGA AAAGGTTCTG TCTCGTGCAT TGATGTACAT TCCACAACTG 
GGGAAACATG CAGAAAGCAT TCTGGAAAAT GGCAGCAGCA GTGGCAGAAA ACTTGCCAAA 
CTTCAGAGAA TTGCTCGCCA GGCTGTCGCT GCACTGTGTG CACTTGGAGG CTTCAAAGAG 
ACAATCAAGA TAGGATCTGA GGTTCAGGTT TTAGGTAGAG GAATATCAGG AAGCATTGGA 
GTAGTGGCTT CTATCAACGA ACAGGAAGGT ATCGCTACAG TCAGATTTCC ACCCATAGAC 
TGTCGAAAGA CTTCTCAAGC GTCAGACACA TTGACTATTC CATTGTCTAG ACTTTGTGTT 
CCAAGGTCAG AGGCACTGCC CCTCCACAAA CTGTCCATTA CTGAAAAGGT GGTACAGGCA 
GTTCAGTCCA TGCTGCTTCC ACAGGAAGGC AGTCTCTCAA TTCACACCTC GCTTCCTGCA 
ACAGGAGATG GCTCTGCACC TGTGATGGCA GTCGTCCGGC TCCTGGCTGA AATAAGAACA 
AGAGCATGCC TGGTCATGGC CCAGCTTTTA GAAGACAGCT TATTTTGTGA AGAATTCATA 
CAGCAGTGTC CAGCAGCTGT TGAAGTTCTT AATCTGGTAG CCCAGGAATG CAGTGCTGGA 
GAGCGACTTG CTGTTGTGGA GGTGCAGTGT GAGCGGCTGA GGATGCTCTA TCGGGACTGC 
GCCCGGCCCC CGCCCCCTCC CCTGCAGGCT GACCGGAGAC AGCCCAAAGA GATAACTTGG 
AGCCCCTCCC GAGTGTTTCC TCCAGTGCGG GCCTGCATGT TCTCATCTCA CCTGACCTCG 
GTCACATTCC TGGCTGACCC CAGCGCTGGG GGAGGTCTGC CCCGGGGCAC CTTTATCTAC 
GCCACCTCAC CTCTGCCAGT TCAGGCCCCA TCTTTTTACT GGGAAATTGA AATTGTTTCC 
TATGGAGATA CTGATGACGA CACTGGACCA ATAGTTTCCT TTGGCTTTGC TACAGAAGCC 
GAGAAACGAG ATGGAGCTTG GACCAACCCA GTGGGCACTT GCCTTTTCCA CAACAATGGC 
CGAGCCGTGC ACTACAACGG CTCCAGTCTG CTCCAGTGGA AGAGCGTTCG CTTAGACGTG 
ACTCTCTCTC CTGGTGACGT GGCAGGAATT GGCTGGGAGA GAACCGAGGG TACTCCACCT 
CCACCGGGGC AGCCAGCCAA GGGCCGGGTG TACTTCACAT ATTGCGGGCA GCGCCTTTCA 
CCATACCTTG AGGATGTCTC TGGTGGGATG TGGCCTGTGG TTCACATTCA GAAAAAGAAC 
ACCAAAACTC GAGCCAACTT TGGCTCCCGC CCATTTGCCT ATGCCGAGGG GCAGGCCCAT 
CGCAACGCAG CTGACCTATG CACTGACCTG GCAGAGGAGA TTAGCGCAAA CTTCGAGGCC 
CTGCCCTTTG CCATGGCATC TGACAGTGAC AACGATGCCG GCACCAGTAT TGCATCAGAC 
CCAGGCACTC ATGGGCCACC GTGCCGGATT GCAGCCGTGG CCACCGCTCA GCAACAATAC 
GATAGTGACA CCTCCTGTCA TTATAAAGTT GAGCTCAGCT ATGAGAATTT CATCACCTCT 
GGCCCAGACC CTCACCCGCC TCCCATTGCA GACGATGAGA GTGATGATGA TGACGATGAT 
GACATTCCAC AGGAGGACCA CTATGCCCTG CTGGTGAAAG CTTGGGAGAC CAAGGTTTTT 
CCCACCATCC GAAGACGCTT CCGGAATGAA GCAGAGAGGA AATCCGGCCT GGACCAGATC 
AAGGGTGCTT TACAACTAGG CATGGTGGAT ATTGCCCGGC AGACGGTGGA ATTTCTATAT 
GAGGAGAATG GCGGCATCCC AAGAGACCTG TATCTTCCCA CCATTGAGGA TATTAAAGAT 
GAAGCAAACA AGTTCACAAT TGACAAAGTT CGAAAAGGTC TCACAGTTGT AACTCGCTCT 
CCAGACAGTA ATAATGTAGC CAGCAGTACC GTTGGAACTG CTTTGCCAAA ATTTGCCATC 
CGTGGGATGC TGAAAACCTT TGGACTTCAT GGAGTCGTCT TAGATGTCGA TTCAGTGAAC 
GAGCTGGTGC AGGTGGAAAC ATATCTCCGG AGTGAGGGGG TGCTGGTGCG GTATTGGTAC 
CCCATTGACA TGTTGGAAAG GCCCCCTGCA GGCTACCGAA GGACTGCCAC CAATGGGCTG 
GTCACACTGG ACAACACCAA CCTTCAAATT CACAGGGAGC TGCTGCGGTG TGAGGCTGCG 
TTGGCCAGGC TCTACTGCCG CATGGCCCTA CTCAACATCT TCGCCCCCAA GTTCCCTCAC 
TTGTTTACCC GCCTCTTCCA CATCCCTGCC ATCCGAGACA TCACTCTGGA GCACCTGCAA 
CTGCTGTCCA ATCAGCTCCT CGCCCCTCCC CTCCCAGATG GCACCATCAG CTCCAGCTCC 
ATCCTCCTGG CGCAGTCCCT GCAGCACTGC ATACACTCCC AGAACTGTTC CGCCACGGAC 
CTCTTTTACC AGGGCAGCTC CCAGACAGTG AGAGAGTGGC TGAGTGTGGC CATCACCCGC 
ACCCTGCACC AGGGCGAGGA GAGCCTTCTG GAGCTCACGA GGCAGATCTG CTCCTTCCTG 
CAGACAGCAC CAGAGCAGTT CCCCTCCGAA GAATTCCCGA TATCCGAATC CAAAGTCAAT 
ATGGATGTTA ATTTTCCCGG GGCGGCTTTT GTTGTGGTGT CTTGTAAAGA AAGTCAATCT 
GGATTCCGCA AAGACTCCTC TTTGTACAAG GCACCCTGGG CACGCGTCCT CGTGTACGGG 
CTTGGCCACA AAGTGAAGCG AACCGGCCAG CTGAACCTCA TCGAGGCTGC CTGTTACCCA 
CGGGATGCGT CCCCGGCCAA CACTGGGCTT GCACCTCCGC CCACAGCTGA CCAGTACCCT 
TCTGTGGTGC TCTCCACAGA CAGGGTGCAC ATCAAGCTAG GAGTGTCTCC CCCTCCTGGA 
GCCGTGCTGG TATTGCATTC TCTGCCCCTG GAGTTCCCGC TGGCTATGGC TTTCGCAGAG 
CAGCTGCTGT CATGGAAGTC GGAGGATGGC GAAGGGAAGT CTGAAGATGA GCCTGACACT 
ATTCCCACCT CAGTCCTCCT GCAGGTGGTG GAGCTGCTAG GAAATTTCCT GTGGACCACA 
GACATGGCAG CCTGCGTGAA GGAACTTGTC TTCCACCTCC TGGCAGAGCT CCTGCGCACG 
GTGCACGCAC TAGAGCAGAG GAAGCACCCC GCCGGCCTGT CCTCCTCCAT TGCCCTGCAG 
CTCAACCCCT GTCTGGCCAT GCTGATGGCC CTGCAGTCAG AGCTGCACAA GCTGTATGAC 
GAGGAGACCC AGAGCTGGGT CTCAGGCAGC ACCTGTGGGG GCTCTGGGGC AGTGGCAACC 
GGCGACCAGG GCAGGTTCTC CACGTACTTT CATGCACTCA TGGAAGGGTG CCTGGCAGTT 
GCCGAAGTGA CCCTGCCTAC CAACATGAGT GTCACAGCCA GTGGAGTGAC CTCAGCAACC 
ACCCCAAACC TCAGTGACTC GTCGTCCTCC TCCTCTTCTT CTCCAGGACA GACACCGCAG 
AGTCCCAGCC TCCTCTCCAA GAGGAAGAAA GTCAAGATGA AGCGGGAAAA GGCCTCCTCC 
TCTGGGAAGC GGCAGTCCTC CCGCACAGTG GAGTCCGACT CCGCCGTGCT CAGCATCGGG 
GGCAGCAAGC CTGAGGACAT GCTCTGGTTC CACCGCGCGC TCACCTTGCT CATCATTCTG 
CGGCACCTCA CCAGGAAGGA CCCCCAGGGC CTGGGTGTGA CCAGCGATGC CGTTGCAGAT 
GCCTGCCAGG CCCTAGTGGG CCCCACTGCC CATAGCCGCC TGCTGGTGAT CTCTGGCATC 
CCTACCCACC TGGACGAGGG CATCGTCAGA GGTGCTATCC GCAAGGCCTG CAATGCCCAC 
GGCGGGGTCT TCAAAGATGA GATCTACATT CCACTGCAGG AAGAAGACCC CAAGAAGCCC 
AAAGACAAGG CCGAAGGTGG CGATGGGAAG GCCGAGCCGG AGAAGACGCT GGGCTTCCCC 
AGTGCAGACA GCCTGGAGGG GAGCACGTCC AGCAGCCTGG CCCCGGCCAT GAGCATCAGT 
GCCTCTGCCT CCACCAGCCA AGCCTCTGTC TGCAGCTCCC AGGGGGTCTC TCAGACCGTC 
AGCGACCTCT CGGTGGACCC ACTGCCGTCC GGCCTGGAGT TGCCCATTCC TCCTGGCCTG 
CTGGAGCCCC ACGTGGTGTC CAGCCAGGAG AGCCTGGATA TTTCCCTGTG CAGCACTGGC 
AGCCTGGGCA GTCTGGGCAG CCTGGGGGAG CCTCTAGACA ATGCGGAGAC AGCCTCGGTG 
TCAGACATGG GCTCCATGTA CACAGTCACT TCCCTGGACA ACCAGTCCCT TGCGGCACGC 
CCCATCAAAG GCTTTGCCGT CGTGGAGATA AGATCCCGAG CCAAGATTGA GAAAATTCGA 
GCAAGTTTAT TTAACAATAA TGATTTAATT GGCTTATCAA GTTTGGATGG TGAAGATGAA 
TTGATGGAAA TGTCCACAGA AGAGATTCTG ACTGTATCTG TAGTGAACCA GAGTTTGTTT 
GATACTCAAG GGAGCCCGGG GTTAGAAGAT TATTTCAATG ATAAGTCAAT TAAAGGTGAG 
AAGCTGGTGC CTGGGGCCAG AGAGGTCCTG ACAGAGATAT TTAAGAGCTG TGCCCACTCA 
GAGCAGATGC TGAGCCTGAC ACCAGCAAAG CCCATCAGGG TCTCTGACAT ATATCTCAGC 
AAGGAGCAGA TCAACTCCCA GACTCCAGGC AACCTCCTCC ACATCTTCTT CACCAATGTC 
CGGCCTCCCA AGAAGGTGCT AGAAGACCAG CTCACCCAGA TCCTGAGGAA ATATGGCGTG 
CCGAAGCCCA AGTTTGACAA GAGCAAGTAC AGCAAAGCTG GGAAGGAGCA GCACCCCGTG 
AAGGTGGTGA GCACCAAGCG GCCCATCACC AAGCCGCCTG CCAAGGACAA AGCCGTGCTC 
AACAGTGTCA GCAGGACTGC CTTAAGTGAG AAGAAACCAA CTGTGAAGCC CAAGTCACCA 
GAAAAGAGCA AACCAGACGA AAAGGATCCA GAAAAGTCGC CCACAAAAAA ACAAGAAGTC 
CCCGAGGAGA AGTACCTGAC CTTAGAAGGG TTTCACAAGT TTGTCATCGA CCGAGCCAAG 
CAGGACATCC GCAGCGTGTG GAGGGCAATT CTGTCTTGTG GCTACGATCT TCATTTTGAG 
AGGTGTGCCT GCATTGATGT CCGACATGCG CAGAAGGCCT CAAGGAAGTG GACCCTGGAG 
ATGGATGTAG CTCTCGTGCA GTACATCAAT CGGCTGTGCC GCCACCTTGC CATCACACCT 
GCAAGGCTCC ACCCCCACGA GGTTTACCTG GACCCTGCCG ATGCCACTGA CCCCAGGGTG 
GCCTGTCTCT TGAATGTGCC CATTGAGAGC CTGCGCCTGC GCTTCGCTTT GCTGCAGTCC 
CTCAACACCA CGCTGGAGAC CTTCTTCCTG CCCCTGGTGG AGCTGCGCCA GACACCCATG 
TACACCCACA GCATCGCGGC CCTTCTGAAG GAGGCCAAAG GGCTGATCTT CTATGACACA 
AAGGTGACCG TGATGAACCG AGTGCTGAAT GCCACTGTGC AGAGGACAGC TGACCACGCG 
GCACCCGAGA TCACCTTGGA CCCATTGGAA ATTGTGGGAG GGGAAATCAG AGCTTCTGAA 
AACTCCTACT TCTGCCAGGC TGCCAGGCAA CTGGCCTCTG TGCCATCCTC TCAGCTCTGC 
GTCAAGCTGG CGAGTGGCGG CGACCCCACC TACGCCTTCA ACATCCGCTT CACCGGGGAG 
GAGGTCCACG GCACCAGTGG CTCCTTCCGC CACTTCCTGT GGCAGGTGTG CAAGGAGCTG 
CAGAGCTCTT CGCTGTCGCT CCTGCTGCTG TGCCCCAGCT CTGCTGTCAA CAAGAACAAG 
GGCAAGTACA TCCTGACGCC CAGCCCCATC ACCTACGGAG AGGAGCAGCT GCTGCACTTC 
CTAGGCCAGT TGCTGGGGAT CGCCATCCGG GCAGATGTCC CCCTTCCCCT GGACCTCCTG 
CCCTCCTTCT GGAAGACTCT GGTGGGGGAG CCCCTGGACC CTGACCAGGA CCTGCAGGAA 
GCAGACATCC TTACCTATAA TTACGTCAAG AAGTTTGAGA GCATCAACGA CGAGAGCGAG 
CTGGAAGCCC TGTGCGCCGA GATTGCCTCC CAGCACCTGG CCACCGAGAG TCCTGACGGC 
CCCAGCAAGC CCTGCTGCAG GTTCACCTAC CTGACCATGA CGGGCGAGGA GGTGGAGCTG 
TGCAGCCGCG GCCGGCACAT CCCTGTGGCG TGGGAGAACA AGGACATCTA CGCTGCAGCC 
ATCCGCAGCC TGCGCTTGCG GGAGCTGCAG AACGTGGAAT GCGTGACAGC CGTGCGCGCA 
GGCCTGGGCT CTATCATCCC CCTGCAGCTG CTCACCACCC TCAGCCCCCT GGAGATGGAG 
CTGCGCACCT GTGGCCTCCC CTACATCAAC CTCGAGTTCC TGAAGGCCCA TACCATGTAC 
CAGGTGGGGC TAATGGAGAC GGACCAACAC ATCGAGTTCT TCTGGGGGGC CCTGGAGACG 
TTCACCCAGG AGGAGCTGTG CAGGTTCATC AAGTTCGCCT GCAACCAGGA GCGCATCCCC 
TTCACCTGCC CCTGTAAAGA TGGGGGCCCC GACACAGCCC ACGTACCCCC CTACCCCATG 
AAGATCGCCC CCCCAGATGG CACAGCAGGT TCCCCAGACT CCCGCTACAT CCGGGTGGAG 
ACCTGCATGT TCATGATCAA GCTCCCCCAG TACTCCTCTT TGGAAATCAT GCTGGAGAAA 
CTGCGCTGTG CCATTCACTA CAGGGAAGAC CCCCTAAGTG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734254.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589929.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X5, mRNA.

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CloneID	OIc83557
Clone ID Related Accession (Same CDS sequence)	XM_021734254.1
Accession Version	XM_021734254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGGTGAGCA CCAAGCGGCC CATCACCAAG 
CCGCCTGCCA AGGACAAAGC CGTGCTCAAC AGTGTCAGCA GGACTGCCTT AAGTGAGAAG 
AAACCAACTG TGAAGCCCAA GTCACCAGAA AAGAGCAAAC CAGACGAAAA GGATCCAGAA 
AAGTCGCCCA CAAAAAAACA AGAAGTCCCC GAGGAGAAGT ACCTGACCTT AGAAGGGTTT 
CACAAGTTTG TCATCGACCG AGCCAAGCAG GACATCCGCA GCGTGTGGAG GGCAATTCTG 
TCTTGTGGCT ACGATCTTCA TTTTGAGAGG TGTGCCTGCA TTGATGTCCG ACATGCGCAG 
AAGGCCTCAA GGAAGTGGAC CCTGGAGATG GATGTAGCTC TCGTGCAGTA CATCAATCGG 
CTGTGCCGCC ACCTTGCCAT CACACCTGCA AGGCTCCACC CCCACGAGGT TTACCTGGAC 
CCTGCCGATG CCACTGACCC CAGGGTGGCC TGTCTCTTGA ATGTGCCCAT TGAGAGCCTG 
CGCCTGCGCT TCGCTTTGCT GCAGTCCCTC AACACCACGC TGGAGACCTT CTTCCTGCCC 
CTGGTGGAGC TGCGCCAGAC ACCCATGTAC ACCCACAGCA TCGCGGCCCT TCTGAAGGAG 
GCCAAAGGGC TGATCTTCTA TGACACAAAG GTGACCGTGA TGAACCGAGT GCTGAATGCC 
ACTGTGCAGA GGACAGCTGA CCACGCGGCA CCCGAGATCA CCTTGGACCC ATTGGAAATT 
GTGGGAGGGG AAATCAGAGC TTCTGAAAAC TCCTACTTCT GCCAGGCTGC CAGGCAACTG 
GCCTCTGTGC CATCCTCTCA GCTCTGCGTC AAGCTGGCGA GTGGCGGCGA CCCCACCTAC 
GCCTTCAACA TCCGCTTCAC CGGGGAGGAG GTCCACGGCA CCAGTGGCTC CTTCCGCCAC 
TTCCTGTGGC AGGTGTGCAA GGAGCTGCAG AGCTCTTCGC TGTCGCTCCT GCTGCTGTGC 
CCCAGCTCTG CTGTCAACAA GAACAAGGGC AAGTACATCC TGACGCCCAG CCCCATCACC 
TACGGAGAGG AGCAGCTGCT GCACTTCCTA GGCCAGTTGC TGGGGATCGC CATCCGGGCA 
GATGTCCCCC TTCCCCTGGA CCTCCTGCCC TCCTTCTGGA AGACTCTGGT GGGGGAGCCC 
CTGGACCCTG ACCAGGACCT GCAGGAAGCA GACATCCTTA CCTATAATTA CGTCAAGAAG 
TTTGAGAGCA TCAACGACGA GAGCGAGCTG GAAGCCCTGT GCGCCGAGAT TGCCTCCCAG 
CACCTGGCCA CCGAGAGTCC TGACGGCCCC AGCAAGCCCT GCTGCAGGTT CACCTACCTG 
ACCATGACGG GCGAGGAGGT GGAGCTGTGC AGCCGCGGCC GGCACATCCC TGTGGCGTGG 
GAGAACAAGG ACATCTACGC TGCAGCCATC CGCAGCCTGC GCTTGCGGGA GCTGCAGAAC 
GTGGAATGCG TGACAGCCGT GCGCGCAGGC CTGGGCTCTA TCATCCCCCT GCAGCTGCTC 
ACCACCCTCA GCCCCCTGGA GATGGAGCTG CGCACCTGTG GCCTCCCCTA CATCAACCTC 
GAGTTCCTGA AGGCCCATAC CATGTACCAG GTGGGGCTAA TGGAGACGGA CCAACACATC 
GAGTTCTTCT GGGGGGCCCT GGAGACGTTC ACCCAGGAGG AGCTGTGCAG GTTCATCAAG 
TTCGCCTGCA ACCAGGAGCG CATCCCCTTC ACCTGCCCCT GTAAAGATGG GGGCCCCGAC 
ACAGCCCACG TACCCCCCTA CCCCATGAAG ATCGCCCCCC CAGATGGCAC AGCAGGTTCC 
CCAGACTCCC GCTACATCCG GGTGGAGACC TGCATGTTCA TGATCAAGCT CCCCCAGTAC 
TCCTCTTTGG AAATCATGCT GGAGAAACTG CGCTGTGCCA TTCACTACAG GGAAGACCCC 
CTAAGTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589929.1
CDS	252..13103
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSLFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVVSTKRPITKPPAKDKAVLNSVSRTALSEKKPTVKPKSPEKSKPDEKDPEKSPTKKQEVPEEKYLTLEGFHKFVIDRAKQDIRSVWRAILSCGYDLHFERCACIDVRHAQKASRKWTLEMDVALVQYINRLCRHLAITPARLHPHEVYLDPADATDPRVACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLKEAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASENSYFCQAARQLASVPSSQLCVKLASGGDPTYAFNIRFTGEEVHGTSGSFRHFLWQVCKELQSSSLSLLLLCPSSAVNKNKGKYILTPSPITYGEEQLLHFLGQLLGIAIRADVPLPLDLLPSFWKTLVGEPLDPDQDLQEADILTYNYVKKFESINDESELEALCAEIASQHLATESPDGPSKPCCRFTYLTMTGEEVELCSRGRHIPVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTTLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALETFTQEELCRFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

Target ORF information:

RefSeq Version	XM_021734254.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734254.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGGTGAGCA CCAAGCGGCC CATCACCAAG 
CCGCCTGCCA AGGACAAAGC CGTGCTCAAC AGTGTCAGCA GGACTGCCTT AAGTGAGAAG 
AAACCAACTG TGAAGCCCAA GTCACCAGAA AAGAGCAAAC CAGACGAAAA GGATCCAGAA 
AAGTCGCCCA CAAAAAAACA AGAAGTCCCC GAGGAGAAGT ACCTGACCTT AGAAGGGTTT 
CACAAGTTTG TCATCGACCG AGCCAAGCAG GACATCCGCA GCGTGTGGAG GGCAATTCTG 
TCTTGTGGCT ACGATCTTCA TTTTGAGAGG TGTGCCTGCA TTGATGTCCG ACATGCGCAG 
AAGGCCTCAA GGAAGTGGAC CCTGGAGATG GATGTAGCTC TCGTGCAGTA CATCAATCGG 
CTGTGCCGCC ACCTTGCCAT CACACCTGCA AGGCTCCACC CCCACGAGGT TTACCTGGAC 
CCTGCCGATG CCACTGACCC CAGGGTGGCC TGTCTCTTGA ATGTGCCCAT TGAGAGCCTG 
CGCCTGCGCT TCGCTTTGCT GCAGTCCCTC AACACCACGC TGGAGACCTT CTTCCTGCCC 
CTGGTGGAGC TGCGCCAGAC ACCCATGTAC ACCCACAGCA TCGCGGCCCT TCTGAAGGAG 
GCCAAAGGGC TGATCTTCTA TGACACAAAG GTGACCGTGA TGAACCGAGT GCTGAATGCC 
ACTGTGCAGA GGACAGCTGA CCACGCGGCA CCCGAGATCA CCTTGGACCC ATTGGAAATT 
GTGGGAGGGG AAATCAGAGC TTCTGAAAAC TCCTACTTCT GCCAGGCTGC CAGGCAACTG 
GCCTCTGTGC CATCCTCTCA GCTCTGCGTC AAGCTGGCGA GTGGCGGCGA CCCCACCTAC 
GCCTTCAACA TCCGCTTCAC CGGGGAGGAG GTCCACGGCA CCAGTGGCTC CTTCCGCCAC 
TTCCTGTGGC AGGTGTGCAA GGAGCTGCAG AGCTCTTCGC TGTCGCTCCT GCTGCTGTGC 
CCCAGCTCTG CTGTCAACAA GAACAAGGGC AAGTACATCC TGACGCCCAG CCCCATCACC 
TACGGAGAGG AGCAGCTGCT GCACTTCCTA GGCCAGTTGC TGGGGATCGC CATCCGGGCA 
GATGTCCCCC TTCCCCTGGA CCTCCTGCCC TCCTTCTGGA AGACTCTGGT GGGGGAGCCC 
CTGGACCCTG ACCAGGACCT GCAGGAAGCA GACATCCTTA CCTATAATTA CGTCAAGAAG 
TTTGAGAGCA TCAACGACGA GAGCGAGCTG GAAGCCCTGT GCGCCGAGAT TGCCTCCCAG 
CACCTGGCCA CCGAGAGTCC TGACGGCCCC AGCAAGCCCT GCTGCAGGTT CACCTACCTG 
ACCATGACGG GCGAGGAGGT GGAGCTGTGC AGCCGCGGCC GGCACATCCC TGTGGCGTGG 
GAGAACAAGG ACATCTACGC TGCAGCCATC CGCAGCCTGC GCTTGCGGGA GCTGCAGAAC 
GTGGAATGCG TGACAGCCGT GCGCGCAGGC CTGGGCTCTA TCATCCCCCT GCAGCTGCTC 
ACCACCCTCA GCCCCCTGGA GATGGAGCTG CGCACCTGTG GCCTCCCCTA CATCAACCTC 
GAGTTCCTGA AGGCCCATAC CATGTACCAG GTGGGGCTAA TGGAGACGGA CCAACACATC 
GAGTTCTTCT GGGGGGCCCT GGAGACGTTC ACCCAGGAGG AGCTGTGCAG GTTCATCAAG 
TTCGCCTGCA ACCAGGAGCG CATCCCCTTC ACCTGCCCCT GTAAAGATGG GGGCCCCGAC 
ACAGCCCACG TACCCCCCTA CCCCATGAAG ATCGCCCCCC CAGATGGCAC AGCAGGTTCC 
CCAGACTCCC GCTACATCCG GGTGGAGACC TGCATGTTCA TGATCAAGCT CCCCCAGTAC 
TCCTCTTTGG AAATCATGCT GGAGAAACTG CGCTGTGCCA TTCACTACAG GGAAGACCCC 
CTAAGTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hectd4 ( XM_021734255.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021589930.1 Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X7, mRNA.

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CloneID	OIc83558
Clone ID Related Accession (Same CDS sequence)	XM_021734255.1
Accession Version	XM_021734255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	probable E3 ubiquitin-protein ligase HECTD4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936668.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGCAGGACT GCCTTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021589930.1
CDS	137..11047
Translation	MGLLLVFPLIQSQSRTDPSLCNITAEVLLNCLRDCQPLSLTKEPADCLNGIETLLCSWLEETSDTGRHIPQKQKENAAAALVALACARGSLKTFVHTVHLLQKQTDLGSLPVADVLYRLLLLEGGPGSPSCLLGGKHIVSWGYEDMLPAPDSNTGSSSENKDADLGRCLTADGLYLYTTNSVGRGVSKLGSGLHGTLRGFVYCRNEELEPGWVAFGSGSLLHRPVSFDNKPHSLFQVIDQNTLQVCQTVAMPANHLPVGSTMSTVHLSSDGTYFYWIWSPASLNEKTPKGHSVFMDIFELVVENGVFVANPLQERTILMRKEGESAKSINEMLLSRLSRYRASPSATLAALTGSTISNTLKEDQAANTSCGLPLKMLRKTPIYTCGTYLVMLVPPPGGSGSSATRSLFGGTSGLSSLKILASSLVYNISDGQFTSRADLIDAAGSSLGRGALVPGLGACYDTVNNMLWTCSNDYIDQWCNPGNQAFHYVCQRLGVSHIITEPKEEAITTNEVINQLLHHVGAMCIHQLNLLATNPNLPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQNHLLSNTILIKPDENDDSDNSLQGETLKVQELKVSILALATQILTGCDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQTLTMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCFPCGTSDQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIPGARCLYLRFDSRCSSQYDYDKSNSLFSVLKLVIYAGPNTNSRKVAEYGGNTLGYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITKEEEACQELLRSKLLQRCQWQVEANGVISPALTPSPSPLPLTIEEDREFTYPSDVLVPPVGNYFDLPRIRLPPGIMIKLREISGRARPQFRPSIKEVIQPDVMEEMVVSCVIKHLNLVDALQSLINFQYQEEHAEEYDLLCKIMGETFKKLNAMERQLQSVAELEQKWQSEVDDAMQGKLENNMPFFYDYHFNENKMKELELLCSMKEVSFDGNDLENMVLSLREKFLQEVNSLIQKPSHPLAKTKTLVKSLMNRAELLLHVTIAAQSGLTRSISGTPAETPACKSASETKVISHAVRQPVFLRSMSAPSDLEMIGNEDLEFTRASQRRRHVTSHRSSSFTLLQSLAIEDSRDKPTYSVLLGQLFAFIGTNPDQAVSSSSFLLAAQTRWRRGNTRKQALVHMRELLTAAVRVGGVTHLVGPVTMVLQGGPRIEELTCGGMVEQVQEAFGETMTSVVSLCARYPIACANSIGLLCTIPYTRSEEKCLVRSGLVQLMDRLCSLSSQTESSSSEKQTKKQKVATMAWAAFQVLANRCVEWEKEEGGSTEAVHSGLARQVSSLLTNHLARATECCGNQAAGNDALQDVLSLLNDLSRSHIGKAILSQPACVSKLLSLLLDQRPSPKLVLIILQLCRAALPLMSVEDCGNVELPPWSYSVPSLNSEQEDPSDPASKIASLLLAKLADYVVPGCQTVLSPTASEPDTTLTKTSPKNSLKGDKDPGEESEAVDGKLSIFIHKREDQSSHEVLQPLLSSSEGRPFRLGTGANMEKVVKMDRDMTKGGCCEVITEEAAAALRKATKWAQSGLIVSIGPPVESVNPETVSGLSTGDKKKTAQTSICRERNSELARTDPVRPFISGHVANSMAAEVIALLHSLLMAPESNAAQIWTTTAEKVLSRALMYIPQLGKHAESILENGSSSGRKLAKLQRIARQAVAALCALGGFKETIKIGSEVQVLGRGISGSIGVVASINEQEGIATVRFPPIDCRKTSQASDTLTIPLSRLCVPRSEALPLHKLSITEKVVQAVQSMLLPQEGSLSIHTSLPATGDGSAPVMAVVRLLAEIRTRACLVMAQLLEDSLFCEEFIQQCPAAVEVLNLVAQECSAGERLAVVEVQCERLRMLYRDCARPPPPPLQADRRQPKEITWSPSRVFPPVRACMFSSHLTSVTFLADPSAGGGLPRGTFIYATSPLPVQAPSFYWEIEIVSYGDTDDDTGPIVSFGFATEAEKRDGAWTNPVGTCLFHNNGRAVHYNGSSLLQWKSVRLDVTLSPGDVAGIGWERTEGTPPPPGQPAKGRVYFTYCGQRLSPYLEDVSGGMWPVVHIQKKNTKTRANFGSRPFAYAEGQAHRNAADLCTDLAEEISANFEALPFAMASDSDNDAGTSIASDPGTHGPPCRIAAVATAQQQYDSDTSCHYKVELSYENFITSGPDPHPPPIADDESDDDDDDDIPQEDHYALLVKAWETKVFPTIRRRFRNEAERKSGLDQIKGALQLGMVDIARQTVEFLYEENGGIPRDLYLPTIEDIKDEANKFTIDKVRKGLTVVTRSPDSNNVASSTVGTALPKFAIRGMLKTFGLHGVVLDVDSVNELVQVETYLRSEGVLVRYWYPIDMLERPPAGYRRTATNGLVTLDNTNLQIHRELLRCEAALARLYCRMALLNIFAPKFPHLFTRLFHIPAIRDITLEHLQLLSNQLLAPPLPDGTISSSSILLAQSLQHCIHSQNCSATDLFYQGSSQTVREWLSVAITRTLHQGEESLLELTRQICSFLQTAPEQFPSEEFPISESKVNMDVNFPGAAFVVVSCKESQSGFRKDSSLYKAPWARVLVYGLGHKVKRTGQLNLIEAACYPRDASPANTGLAPPPTADQYPSVVLSTDRVHIKLGVSPPPGAVLVLHSLPLEFPLAMAFAEQLLSWKSEDGEGKSEDEPDTIPTSVLLQVVELLGNFLWTTDMAACVKELVFHLLAELLRTVHALEQRKHPAGLSSSIALQLNPCLAMLMALQSELHKLYDEETQSWVSGSTCGGSGAVATGDQGRFSTYFHALMEGCLAVAEVTLPTNMSVTASGVTSATTPNLSDSSSSSSSSPGQTPQSPSLLSKRKKVKMKREKASSSGKRQSSRTVESDSAVLSIGGSKPEDMLWFHRALTLLIILRHLTRKDPQGLGVTSDAVADACQALVGPTAHSRLLVISGIPTHLDEGIVRGAIRKACNAHGGVFKDEIYIPLQEEDPKKPKDKAEGGDGKAEPEKTLGFPSADSLEGSTSSSLAPAMSISASASTSQASVCSSQGVSQTVSDLSVDPLPSGLELPIPPGLLEPHVVSSQESLDISLCSTGSLGSLGSLGEPLDNAETASVSDMGSMYTVTSLDNQSLAARPIKGFAVVEIRSRAKIEKIRASLFNNNDLIGLSSLDGEDELMEMSTEEILTVSVVNQSLFDTQGSPGLEDYFNDKSIKGEKLVPGAREVLTEIFKSCAHSEQMLSLTPAKPIRVSDIYLSKEQINSQTPGNLLHIFFTNVRPPKKVLEDQLTQILRKYGVPKPKFDKSKYSKAGKEQHPVKVQDCLK

Target ORF information:

RefSeq Version	XM_021734255.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT domain E3 ubiquitin protein ligase 4 (Hectd4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021734255.1

ORF Insert Sequence:

ATGGGGCTCC TGCTGGTCTT CCCCCTTATT CAGTCCCAGA GTAGAACAGA TCCTTCTCTC 
TGTAACATAA CTGCTGAGGT GCTATTGAAC TGTCTTCGTG ACTGCCAGCC TTTGAGCCTG 
ACCAAGGAGC CCGCTGACTG TCTCAATGGA ATTGAAACTT TGCTGTGCTC TTGGCTGGAG 
GAGACTTCTG ACACAGGCCG CCACATTCCA CAGAAGCAAA AAGAAAATGC TGCTGCTGCC 
CTGGTGGCTT TGGCATGTGC CAGGGGATCA TTGAAAACTT TTGTCCACAC AGTCCACCTG 
TTGCAGAAAC AGACTGATCT TGGGTCCCTT CCTGTTGCTG ATGTACTATA TAGACTGTTG 
CTTTTGGAAG GGGGGCCTGG ATCCCCCTCC TGTTTACTTG GGGGCAAACA CATAGTCTCA 
TGGGGGTATG AAGACATGCT GCCTGCGCCC GATAGCAACA CTGGCTCCAG TTCTGAGAAT 
AAAGATGCTG ATTTGGGACG CTGTCTCACT GCAGACGGTC TTTACCTGTA CACTACTAAC 
TCAGTTGGAA GAGGAGTAAG CAAATTGGGG TCTGGATTAC ATGGTACTCT AAGAGGGTTT 
GTGTACTGCC GGAATGAGGA GCTGGAGCCA GGGTGGGTGG CTTTTGGCAG TGGCAGTCTT 
CTCCACAGGC CTGTGTCTTT CGATAATAAA CCTCACTCCC TGTTCCAGGT CATTGATCAG 
AACACCCTTC AGGTGTGCCA GACAGTGGCC ATGCCAGCCA ATCACCTCCC TGTTGGCAGT 
ACTATGAGCA CCGTGCACCT CTCTTCAGAT GGTACCTACT TCTATTGGAT CTGGTCTCCT 
GCCAGTTTGA ATGAAAAAAC ACCAAAGGGA CATTCTGTCT TCATGGACAT TTTTGAACTT 
GTGGTTGAAA ATGGTGTATT TGTAGCCAAC CCACTTCAGG AACGCACAAT TCTAATGCGA 
AAAGAGGGTG AATCTGCCAA AAGCATTAAT GAGATGCTTC TGAGTAGACT TTCTCGGTAT 
AGAGCTTCCC CATCAGCGAC CCTCGCAGCC CTGACTGGGT CCACCATCTC CAACACGCTG 
AAAGAGGATC AAGCAGCAAA TACTAGCTGT GGGCTACCTT TGAAAATGCT CCGGAAGACA 
CCCATTTATA CCTGTGGCAC ATACCTGGTC ATGCTGGTCC CACCTCCAGG AGGATCAGGC 
AGCTCAGCTA CTCGTTCTCT TTTTGGTGGA ACAAGTGGCT TGTCATCTTT AAAAATCCTA 
GCCTCCAGCT TGGTGTATAA TATTTCGGAT GGTCAGTTCA CAAGTCGTGC GGATCTCATA 
GATGCTGCTG GAAGTTCACT GGGTCGTGGT GCTCTTGTAC CAGGATTGGG CGCCTGTTAT 
GATACAGTGA ACAATATGCT GTGGACATGC TCAAATGATT ACATCGATCA GTGGTGTAAT 
CCTGGGAATC AAGCCTTCCA TTATGTGTGC CAGAGGCTGG GAGTCAGCCA CATCATCACT 
GAACCTAAAG AAGAGGCTAT AACCACAAAT GAGGTTATAA ACCAGTTACT GCACCATGTT 
GGTGCCATGT GCATACACCA GCTCAATCTC TTGGCCACCA ACCCCAATCT TCCCATCACA 
AGTGTCTTGG GCAAGCAGCA TCCAATTGAA GCCCACCATC TGAGCAGCAT TTGTGACATC 
ATGGAGAAGG CTATGGTTAA TGGAGATACC TGTATCATAC GCTGCATTCT CGTTGTCTTT 
CAGGTGGTGT TTAAATTTTT CTTCAGCCCA CAAACTGAAA GGAACCGAGA CATCATTCGA 
CGGTCAGGAC TGCTTCTATG GCAGTTGTTG ATGGCACCAA AAGATCAAAT CTGTCCTGAA 
ATTCAGAAGG AAGTCTGTCT TGCCATCAGC TCTGGTTTAA ATATCTTGTA CCCAGGCGAA 
ACTGAAATCA ATAACTTACT TAAGTTGGTC TTGACAGAAG GAGAGAGAAA CAGTGGACTG 
TCCCAGCTTC GAGATGTGAT CCTAACCAAT TTAGCTGAAC AGCTTCAAAA CAACCGATTT 
GGCAGTGATG AAGATGATCA TTACAGACTA AATGATGAAC TTTTACACTA CATTCTGAAG 
ATTGTTGTAC GAGAATCCTG TATTTTAATC ACCAAGTGCC AAACTGTCTC TAAAGATGAT 
TTTCAAAAGC TCCTTTCAAC TGTGCCTGCT GCATCCTCCT GCCTGCGCTA TCTGATGGCA 
GTTCAGAATC ACCTTCTCAG TAACACTATT TTGATTAAAC CTGATGAGAA CGATGACAGT 
GACAACTCCT TGCAGGGAGA GACATTGAAG GTACAGGAGC TAAAAGTCAG TATTTTGGCT 
CTTGCCACCC AAATCCTGAC TGGATGTGAT GAAGTGTTGG AAATGCTACA GCAGGTCACC 
ACTGCCCTCA TAAACAGTGA CATAGCAGAT CGGGAGCAGA GGTTAAAAGG CTTGGAACAA 
GTTACCAAGG CCACTATGCT CGGTCACCTT CTCCCAGTGT TACTGACCTC CCTGATGCAC 
CCCAATCTGC AGACCTTGAC CATGGCCGAC GCCTTGATGC CTCAGCTGGT ACAGCTGGTG 
CTCTACACCA GCCAGACGGC TTTGCTGCTT AAAACTCAGT GTCCAGTGTT TGCTGAAGTG 
GGCTGCTTCC CATGTGGCAC ATCCGACCAG AAGTGTAGGC TGTTCCCGGA TGAGAGAATG 
CTGGAAGAAA AAGAGGAGCC AGGCTTCCTC ACTGGCCTAA AGATTCCCGC CCCCTGGGCT 
GCTGGGAAAA CCGTGGAAAC AGTACACCCT GTCAGAGACA ACTACAAGTT TAAAGAAACT 
GTTCATATCC CAGGAGCTCG CTGCCTGTAT CTGAGATTTG ATAGCAGATG CTCTTCACAA 
TATGACTATG ACAAATCTAA CAGTCTATTT TCTGTTTTGA AGTTGGTGAT ATATGCGGGG 
CCTAACACAA ACAGTAGGAA GGTTGCTGAA TATGGAGGCA ATACTCTGGG ATATGGCAGC 
CGTAGTGTCT TAGGAACTGG TTGGCCGAAG GACTTGGTGA AGGTGGAAGG AGATACAGTC 
ACCTTCTCCT TTGAAATGAG AAGTGGCCGT GAACACAACA CTCCTGATAA AGCTATGTGG 
GGCTTTGCGT GCACAGTTCG CGCTCAGGAG TCCTCGGAGG ACGTCTCAGG AGGCCTGCCT 
TTTCTGGTGG ACCTGGCTTT AGGTCTGTCT GTGTTAGCTT GTTCCATGTT AAGAATCCTG 
TACAATGGAC CAGAAATTAC CAAAGAAGAG GAAGCCTGTC AGGAGCTTTT GCGGTCCAAA 
CTTTTACAAA GGTGCCAGTG GCAGGTGGAG GCCAATGGCG TCATCTCGCC TGCCCTCACC 
CCAAGTCCCT CTCCACTGCC TCTGACCATA GAGGAAGACC GAGAGTTCAC CTACCCCTCG 
GATGTCCTCG TGCCTCCTGT TGGGAACTAC TTTGACCTGC CCCGGATCCG GCTGCCTCCA 
GGAATCATGA TAAAGCTCAG GGAAATTTCT GGGCGAGCTA GACCTCAATT TAGACCAAGT 
ATAAAGGAAG TGATTCAGCC AGATGTGATG GAGGAAATGG TGGTATCATG TGTTATTAAG 
CACTTGAACT TGGTCGATGC ACTGCAATCC CTAATAAATT TCCAATATCA AGAAGAACAT 
GCTGAGGAAT ATGACTTACT ATGTAAAATT ATGGGAGAGA CCTTTAAGAA ACTCAATGCC 
ATGGAGAGAC AGCTGCAGAG TGTTGCAGAA CTGGAACAGA AGTGGCAAAG TGAGGTTGAT 
GATGCCATGC AGGGGAAATT GGAGAACAAC ATGCCTTTCT TTTATGATTA CCATTTTAAT 
GAGAACAAAA TGAAAGAACT AGAACTTTTG TGTTCAATGA AGGAAGTCTC CTTTGATGGA 
AATGATCTTG AAAACATGGT CCTATCACTG AGGGAGAAGT TCCTACAAGA AGTGAATTCT 
CTTATTCAGA AGCCCTCTCA TCCACTGGCT AAAACGAAGA CATTAGTGAA GAGTCTAATG 
AACCGTGCTG AGCTGCTGCT GCATGTCACC ATTGCGGCCC AATCTGGCCT CACCAGGAGC 
ATCTCGGGAA CCCCTGCAGA GACACCGGCT TGTAAATCAG CTTCTGAAAC AAAGGTGATA 
TCTCATGCCG TCCGGCAGCC TGTTTTTCTC CGTAGTATGT CGGCTCCTTC TGATCTAGAA 
ATGATTGGTA ATGAAGATTT GGAATTTACT AGAGCAAGTC AGAGGCGTCG ACATGTGACC 
AGCCACCGGA GCAGCTCCTT TACACTCCTG CAATCGTTGG CCATTGAGGA CAGCAGGGAC 
AAGCCCACCT ATAGTGTCCT GCTGGGGCAG CTGTTTGCTT TCATTGGCAC CAATCCCGAC 
CAAGCTGTGT CCAGCAGCAG CTTCCTTCTT GCTGCACAGA CGAGGTGGCG GAGGGGAAAC 
ACACGCAAGC AGGCCCTGGT GCACATGCGA GAGCTGCTCA CAGCTGCCGT GCGCGTTGGG 
GGAGTGACAC ATCTTGTGGG GCCAGTGACT ATGGTCCTCC AGGGAGGACC CAGGATTGAA 
GAGCTCACCT GTGGTGGGAT GGTTGAGCAG GTTCAGGAAG CCTTTGGCGA AACCATGACT 
TCGGTTGTGT CTCTGTGTGC CCGTTACCCT ATCGCTTGTG CAAATAGCAT TGGACTCTTG 
TGTACCATAC CTTATACCAG GAGTGAGGAG AAGTGCCTGG TGCGCAGTGG CCTTGTCCAG 
CTCATGGATC GACTGTGCAG CTTGAGCAGT CAGACCGAGT CCAGCTCCAG TGAGAAACAA 
ACCAAGAAGC AGAAAGTGGC CACCATGGCC TGGGCTGCCT TCCAGGTGCT GGCCAACCGC 
TGTGTCGAGT GGGAAAAAGA GGAAGGTGGC TCCACAGAGG CTGTGCACTC GGGGCTGGCT 
CGGCAGGTGT CCAGCCTTCT CACGAATCAT CTTGCCCGAG CCACTGAGTG CTGTGGCAAC 
CAGGCTGCAG GAAATGATGC TCTGCAGGAT GTCCTGAGTC TGCTCAACGA CCTCTCGAGG 
AGCCACATAG GGAAAGCCAT CCTGAGCCAG CCAGCTTGTG TGTCCAAGCT CCTCTCCCTG 
CTGCTTGACC AGCGCCCATC TCCAAAATTG GTCCTTATCA TTCTTCAACT GTGCCGGGCG 
GCACTGCCAT TGATGAGCGT AGAGGACTGT GGAAATGTGG AACTCCCGCC ATGGAGCTAT 
TCTGTCCCCT CCCTAAACAG TGAGCAGGAG GATCCCAGTG ACCCAGCTTC CAAGATCGCC 
TCCTTGCTCT TAGCAAAGCT GGCAGATTAC GTGGTCCCAG GATGTCAGAC AGTTCTTTCT 
CCAACTGCTT CTGAACCGGA TACCACATTA ACAAAAACCA GTCCCAAGAA TTCTTTAAAA 
GGAGATAAAG ATCCTGGAGA AGAGAGTGAA GCAGTGGACG GCAAGCTCTC CATATTTATC 
CACAAACGGG AAGACCAGTC CTCCCACGAA GTTCTCCAAC CATTACTAAG TAGCTCAGAA 
GGGCGGCCCT TCCGACTTGG TACTGGTGCC AACATGGAGA AAGTTGTGAA GATGGATAGA 
GACATGACCA AGGGTGGCTG TTGCGAAGTG ATCACAGAAG AGGCTGCAGC CGCCCTTCGG 
AAAGCCACCA AGTGGGCACA GTCAGGCCTC ATCGTCAGCA TTGGGCCACC AGTAGAGTCT 
GTCAATCCAG AGACTGTGAG TGGACTATCC ACAGGTGACA AAAAGAAAAC TGCCCAAACC 
TCCATCTGCA GAGAGAGGAA CTCAGAACTC GCCAGGACTG ACCCTGTCCG ACCCTTCATC 
AGTGGGCATG TGGCAAACAG CATGGCTGCA GAAGTGATCG CTTTGCTTCA TAGCTTGCTA 
ATGGCACCTG AATCTAACGC GGCTCAAATA TGGACCACAA CAGCAGAAAA GGTTCTGTCT 
CGTGCATTGA TGTACATTCC ACAACTGGGG AAACATGCAG AAAGCATTCT GGAAAATGGC 
AGCAGCAGTG GCAGAAAACT TGCCAAACTT CAGAGAATTG CTCGCCAGGC TGTCGCTGCA 
CTGTGTGCAC TTGGAGGCTT CAAAGAGACA ATCAAGATAG GATCTGAGGT TCAGGTTTTA 
GGTAGAGGAA TATCAGGAAG CATTGGAGTA GTGGCTTCTA TCAACGAACA GGAAGGTATC 
GCTACAGTCA GATTTCCACC CATAGACTGT CGAAAGACTT CTCAAGCGTC AGACACATTG 
ACTATTCCAT TGTCTAGACT TTGTGTTCCA AGGTCAGAGG CACTGCCCCT CCACAAACTG 
TCCATTACTG AAAAGGTGGT ACAGGCAGTT CAGTCCATGC TGCTTCCACA GGAAGGCAGT 
CTCTCAATTC ACACCTCGCT TCCTGCAACA GGAGATGGCT CTGCACCTGT GATGGCAGTC 
GTCCGGCTCC TGGCTGAAAT AAGAACAAGA GCATGCCTGG TCATGGCCCA GCTTTTAGAA 
GACAGCTTAT TTTGTGAAGA ATTCATACAG CAGTGTCCAG CAGCTGTTGA AGTTCTTAAT 
CTGGTAGCCC AGGAATGCAG TGCTGGAGAG CGACTTGCTG TTGTGGAGGT GCAGTGTGAG 
CGGCTGAGGA TGCTCTATCG GGACTGCGCC CGGCCCCCGC CCCCTCCCCT GCAGGCTGAC 
CGGAGACAGC CCAAAGAGAT AACTTGGAGC CCCTCCCGAG TGTTTCCTCC AGTGCGGGCC 
TGCATGTTCT CATCTCACCT GACCTCGGTC ACATTCCTGG CTGACCCCAG CGCTGGGGGA 
GGTCTGCCCC GGGGCACCTT TATCTACGCC ACCTCACCTC TGCCAGTTCA GGCCCCATCT 
TTTTACTGGG AAATTGAAAT TGTTTCCTAT GGAGATACTG ATGACGACAC TGGACCAATA 
GTTTCCTTTG GCTTTGCTAC AGAAGCCGAG AAACGAGATG GAGCTTGGAC CAACCCAGTG 
GGCACTTGCC TTTTCCACAA CAATGGCCGA GCCGTGCACT ACAACGGCTC CAGTCTGCTC 
CAGTGGAAGA GCGTTCGCTT AGACGTGACT CTCTCTCCTG GTGACGTGGC AGGAATTGGC 
TGGGAGAGAA CCGAGGGTAC TCCACCTCCA CCGGGGCAGC CAGCCAAGGG CCGGGTGTAC 
TTCACATATT GCGGGCAGCG CCTTTCACCA TACCTTGAGG ATGTCTCTGG TGGGATGTGG 
CCTGTGGTTC ACATTCAGAA AAAGAACACC AAAACTCGAG CCAACTTTGG CTCCCGCCCA 
TTTGCCTATG CCGAGGGGCA GGCCCATCGC AACGCAGCTG ACCTATGCAC TGACCTGGCA 
GAGGAGATTA GCGCAAACTT CGAGGCCCTG CCCTTTGCCA TGGCATCTGA CAGTGACAAC 
GATGCCGGCA CCAGTATTGC ATCAGACCCA GGCACTCATG GGCCACCGTG CCGGATTGCA 
GCCGTGGCCA CCGCTCAGCA ACAATACGAT AGTGACACCT CCTGTCATTA TAAAGTTGAG 
CTCAGCTATG AGAATTTCAT CACCTCTGGC CCAGACCCTC ACCCGCCTCC CATTGCAGAC 
GATGAGAGTG ATGATGATGA CGATGATGAC ATTCCACAGG AGGACCACTA TGCCCTGCTG 
GTGAAAGCTT GGGAGACCAA GGTTTTTCCC ACCATCCGAA GACGCTTCCG GAATGAAGCA 
GAGAGGAAAT CCGGCCTGGA CCAGATCAAG GGTGCTTTAC AACTAGGCAT GGTGGATATT 
GCCCGGCAGA CGGTGGAATT TCTATATGAG GAGAATGGCG GCATCCCAAG AGACCTGTAT 
CTTCCCACCA TTGAGGATAT TAAAGATGAA GCAAACAAGT TCACAATTGA CAAAGTTCGA 
AAAGGTCTCA CAGTTGTAAC TCGCTCTCCA GACAGTAATA ATGTAGCCAG CAGTACCGTT 
GGAACTGCTT TGCCAAAATT TGCCATCCGT GGGATGCTGA AAACCTTTGG ACTTCATGGA 
GTCGTCTTAG ATGTCGATTC AGTGAACGAG CTGGTGCAGG TGGAAACATA TCTCCGGAGT 
GAGGGGGTGC TGGTGCGGTA TTGGTACCCC ATTGACATGT TGGAAAGGCC CCCTGCAGGC 
TACCGAAGGA CTGCCACCAA TGGGCTGGTC ACACTGGACA ACACCAACCT TCAAATTCAC 
AGGGAGCTGC TGCGGTGTGA GGCTGCGTTG GCCAGGCTCT ACTGCCGCAT GGCCCTACTC 
AACATCTTCG CCCCCAAGTT CCCTCACTTG TTTACCCGCC TCTTCCACAT CCCTGCCATC 
CGAGACATCA CTCTGGAGCA CCTGCAACTG CTGTCCAATC AGCTCCTCGC CCCTCCCCTC 
CCAGATGGCA CCATCAGCTC CAGCTCCATC CTCCTGGCGC AGTCCCTGCA GCACTGCATA 
CACTCCCAGA ACTGTTCCGC CACGGACCTC TTTTACCAGG GCAGCTCCCA GACAGTGAGA 
GAGTGGCTGA GTGTGGCCAT CACCCGCACC CTGCACCAGG GCGAGGAGAG CCTTCTGGAG 
CTCACGAGGC AGATCTGCTC CTTCCTGCAG ACAGCACCAG AGCAGTTCCC CTCCGAAGAA 
TTCCCGATAT CCGAATCCAA AGTCAATATG GATGTTAATT TTCCCGGGGC GGCTTTTGTT 
GTGGTGTCTT GTAAAGAAAG TCAATCTGGA TTCCGCAAAG ACTCCTCTTT GTACAAGGCA 
CCCTGGGCAC GCGTCCTCGT GTACGGGCTT GGCCACAAAG TGAAGCGAAC CGGCCAGCTG 
AACCTCATCG AGGCTGCCTG TTACCCACGG GATGCGTCCC CGGCCAACAC TGGGCTTGCA 
CCTCCGCCCA CAGCTGACCA GTACCCTTCT GTGGTGCTCT CCACAGACAG GGTGCACATC 
AAGCTAGGAG TGTCTCCCCC TCCTGGAGCC GTGCTGGTAT TGCATTCTCT GCCCCTGGAG 
TTCCCGCTGG CTATGGCTTT CGCAGAGCAG CTGCTGTCAT GGAAGTCGGA GGATGGCGAA 
GGGAAGTCTG AAGATGAGCC TGACACTATT CCCACCTCAG TCCTCCTGCA GGTGGTGGAG 
CTGCTAGGAA ATTTCCTGTG GACCACAGAC ATGGCAGCCT GCGTGAAGGA ACTTGTCTTC 
CACCTCCTGG CAGAGCTCCT GCGCACGGTG CACGCACTAG AGCAGAGGAA GCACCCCGCC 
GGCCTGTCCT CCTCCATTGC CCTGCAGCTC AACCCCTGTC TGGCCATGCT GATGGCCCTG 
CAGTCAGAGC TGCACAAGCT GTATGACGAG GAGACCCAGA GCTGGGTCTC AGGCAGCACC 
TGTGGGGGCT CTGGGGCAGT GGCAACCGGC GACCAGGGCA GGTTCTCCAC GTACTTTCAT 
GCACTCATGG AAGGGTGCCT GGCAGTTGCC GAAGTGACCC TGCCTACCAA CATGAGTGTC 
ACAGCCAGTG GAGTGACCTC AGCAACCACC CCAAACCTCA GTGACTCGTC GTCCTCCTCC 
TCTTCTTCTC CAGGACAGAC ACCGCAGAGT CCCAGCCTCC TCTCCAAGAG GAAGAAAGTC 
AAGATGAAGC GGGAAAAGGC CTCCTCCTCT GGGAAGCGGC AGTCCTCCCG CACAGTGGAG 
TCCGACTCCG CCGTGCTCAG CATCGGGGGC AGCAAGCCTG AGGACATGCT CTGGTTCCAC 
CGCGCGCTCA CCTTGCTCAT CATTCTGCGG CACCTCACCA GGAAGGACCC CCAGGGCCTG 
GGTGTGACCA GCGATGCCGT TGCAGATGCC TGCCAGGCCC TAGTGGGCCC CACTGCCCAT 
AGCCGCCTGC TGGTGATCTC TGGCATCCCT ACCCACCTGG ACGAGGGCAT CGTCAGAGGT 
GCTATCCGCA AGGCCTGCAA TGCCCACGGC GGGGTCTTCA AAGATGAGAT CTACATTCCA 
CTGCAGGAAG AAGACCCCAA GAAGCCCAAA GACAAGGCCG AAGGTGGCGA TGGGAAGGCC 
GAGCCGGAGA AGACGCTGGG CTTCCCCAGT GCAGACAGCC TGGAGGGGAG CACGTCCAGC 
AGCCTGGCCC CGGCCATGAG CATCAGTGCC TCTGCCTCCA CCAGCCAAGC CTCTGTCTGC 
AGCTCCCAGG GGGTCTCTCA GACCGTCAGC GACCTCTCGG TGGACCCACT GCCGTCCGGC 
CTGGAGTTGC CCATTCCTCC TGGCCTGCTG GAGCCCCACG TGGTGTCCAG CCAGGAGAGC 
CTGGATATTT CCCTGTGCAG CACTGGCAGC CTGGGCAGTC TGGGCAGCCT GGGGGAGCCT 
CTAGACAATG CGGAGACAGC CTCGGTGTCA GACATGGGCT CCATGTACAC AGTCACTTCC 
CTGGACAACC AGTCCCTTGC GGCACGCCCC ATCAAAGGCT TTGCCGTCGT GGAGATAAGA 
TCCCGAGCCA AGATTGAGAA AATTCGAGCA AGTTTATTTA ACAATAATGA TTTAATTGGC 
TTATCAAGTT TGGATGGTGA AGATGAATTG ATGGAAATGT CCACAGAAGA GATTCTGACT 
GTATCTGTAG TGAACCAGAG TTTGTTTGAT ACTCAAGGGA GCCCGGGGTT AGAAGATTAT 
TTCAATGATA AGTCAATTAA AGGTGAGAAG CTGGTGCCTG GGGCCAGAGA GGTCCTGACA 
GAGATATTTA AGAGCTGTGC CCACTCAGAG CAGATGCTGA GCCTGACACC AGCAAAGCCC 
ATCAGGGTCT CTGACATATA TCTCAGCAAG GAGCAGATCA ACTCCCAGAC TCCAGGCAAC 
CTCCTCCACA TCTTCTTCAC CAATGTCCGG CCTCCCAAGA AGGTGCTAGA AGACCAGCTC 
ACCCAGATCC TGAGGAAATA TGGCGTGCCG AAGCCCAAGT TTGACAAGAG CAAGTACAGC 
AAAGCTGGGA AGGAGCAGCA CCCCGTGAAG GTGCAGGACT GCCTTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Hectd4 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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