Prpf8 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prpf8
Entrez Gene ID	101959153
Full Name	pre-mRNA processing factor 8
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021728159.1	XP_021583834.1	pre-mRNA-processing-splicing factor 8
XM_005327904.2	XP_005327961.1	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems
Gene Ontology

The following Prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc58916	XM_005327904.2 Latest version!	Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OIc58916	XM_021728159.1 Latest version!	Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf8 ( XM_021728159.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021583834.1 Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X2, mRNA.

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CloneID	OIc58916
Clone ID Related Accession (Same CDS sequence)	XM_021728159.1 , XM_005327904.2
Accession Version	XM_021728159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCAG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGACT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CCAGAAAATG GCAGCAGTTG 
CAGGCCAAAC GGTATGCAGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAT 
ATGCCTCCAG AACATGTCAG GAAGATCATT CGAGACCACG GAGACATGAC CAACAGAAAG 
TTTCGCCATG ACAAAAGAGT CTATCTGGGT GCCCTAAAGT ACATGCCCCA TGCGGTCCTC 
AAACTCCTGG AGAATATGCC TATGCCTTGG GAACAGATCC GGGATGTGCC TGTGCTGTAC 
CATATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCTT GGGTCATTGA GCCAGTCTAT 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTTAAGAGGA TGCGCTTCCC TCCTTTTGAT GATGAGGAGC CCCCCTTGGA CTATGCTGAT 
AACATTTTAG ATGTTGAGCC TCTTGAAGCC ATTCAGCTAG AGTTGGACCC TGAGGAGGAT 
GCACCTGTGT TGGACTGGTT TTATGATCAC CAGCCATTGA GAGACAGTAG AAAGTATGTT 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCTA TGATGTCTAC TCTATACCGC 
CTGGCCAATC AGCTCCTAAC AGATTTGGTG GACGACAATT ACTTCTATCT TTTTGATCTA 
AAGGCCTTCT TTACCTCCAA GGCGCTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGATTGGA ATGAATTTAA TGATATTAAC 
AAAATCATTA TTCGGCAGCC TATCCGCACT GAATATAAGA TTGCTTTTCC TTACCTATAC 
AACAACCTTC CACATCACGT TCATCTCACC TGGTATCATA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA TCCAATCTCT 
CACAGGCATT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ATGATGAAGA ATTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CTTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCTC TCCTTTGGGC CCCACGGCCC TTCAATCTTC GCTCTGGCCG CACCCGCCGG 
GCTCTGGACA TACCCCTTGT CAAGAATTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTCAAAGTGA GAGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCTTGAAA 
CATCGTCCCC CTAAGGCTCA AAAGAAGAGG TATCTGTTCC GCTCCTTCAA AGCTACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAAGTTGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAAAACCTTA ACTACCTGCA CCTGGACTAT 
AACTTCAACC TCAAACCTGT CAAGACACTT ACCACTAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCATTTGTG TCGGGAAGTA CTGCGTTTAA CTAAGCTGGT TGTAGACAGT 
CACGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTTCAGC TGGCAGATGG ATTACAGTAT 
ATATTTGCCC ATGTGGGGCA GCTGACAGGC ATGTATCGTT ACAAATACAA ACTGATGAGA 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGTGTCTG GCTATTTTTC 
ATGCGTGGCA TTACTCCTTT GCTAGAGCGA TGGCTAGGCA ATCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCAAAA ACAGTAACGA AGCAGCGAGT GGAGTCACAT 
TTTGATCTTG AACTTCGGGC AGCTGTGATG CATGACATTC TGGATATGAT GCCTGAGGGG 
ATCAAACAGA ATAAGGCCCG GACAATCCTG CAGCATCTCA GCGAAGCCTG GCGTTGCTGG 
AAGGCCAACA TTCCCTGGAA AGTCCCCGGA CTACCAACAC CCATAGAAAA TATGATCCTT 
CGATATGTGA AAGCCAAGGC TGACTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGCCGTG GGGCCACTGT AGACAAGACT GTTTGCAAAA AGAACTTGGG TCGCCTTACT 
CGATTGTACC TGAAGGCAGA ACAGGAGCGA CAGCACAATT ACCTAAAGGA TGGGCCTTAC 
ATCACGGCAG AGGAAGCAGT GGCAGTGTAT ACCACTACTG TGCATTGGTT GGAAAGCCGG 
AGGTTCTCGC CCATTCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTAATT 
TTGGCTTTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAAGAGT TAGGTCTGAT TGAGCAAGCC TATGACAACC CCCATGAGGC ATTGTCTCGC 
ATCAAGCGTC ACCTCCTTAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA ATTCATGGAT 
CTTTATAGTC ACCTGGTTCC AGTGTATGAT GTGGAACCAC TGGAGAAGAT AACTGATGCC 
TACTTGGACC AGTATCTGTG GTATGAAGCA GACAAGCGCC GCCTTTTCCC ACCCTGGATC 
AAACCTGCTG ATACGGAACC ACCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACTTGCAGG ATGTGTGGGA AACCAGTGAG GGTGAGTGCA ATGTCATGCT GGAATCACGC 
TTTGAGAAAA TGTATGAAAA GATTGATTTG ACCCTGCTCA ATAGGCTGCT GCGACTCATT 
GTGGATCACA ACATAGCTGA CTACATGACA GCTAAGAACA ATGTTGTCAT CAACTATAAG 
GATATGAACC ATACTAATTC ATACGGGATC ATCAGAGGAC TGCAGTTTGC CTCTTTCATT 
GTGCAATACT ATGGTCTGGT GATGGATTTG CTGGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTTAGTT TCCAGGACAT AGCCACTGAA 
GCTGCTCATC CCATCCGACT CTTCTGTAGA TACATTGATC GAATTCATAT TTTCTTCAGG 
TTCACTGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TAACAGAGCA CCCTGACCCC 
AACAATGAAA ACATCGTTGG CTATAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGCCTCATGA AGCATGATGT CAATTTAGGT CGAGCAGTAT TTTGGGACAT CAAGAACCGT 
CTGCCACGAT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCTGT GTATAGTAAA 
GACAACCCTA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAATGC 
CGCACCAGCT ATGAGGAGTT TACACACAAG GATGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAAC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGATTC 
CACAACCGTG TACGTCAAAT TCTGATGGCT TCTGGCTCTA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACTCAGGAAC TCCTAGACTT GCTTGTGAAG TGTGAGAACA AGATCCAGAC ACGTATAAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCTCCTG TTGTATTCTA CACCCCTAAG 
GAGTTGGGAG GACTGGGCAT GCTGTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATT ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTATACCGC TACATACAGC CATGGGAGAG TGAGTTCATC 
GATTCTCAGC GGGTCTGGGC TGAATATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGGCTGA CATTAGAAGA TCTAGAGGAC TCTTGGGATC GTGGCATTCC TCGAATCAAT 
ACTCTCTTCC AGAAGGATCG GCACACATTA GCTTATGATA AGGGTTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAA AACCCTTTTT GGTGGACACA CCAGCGACAT 
GATGGGAAGC TCTGGAACCT AAACAATTAC CGTACAGATA TGATTCAAGC CCTGGGTGGT 
GTAGAAGGCA TTCTGGAACA CACACTGTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTTTTCTGGG AGAAGGCTAG TGGCTTTGAA GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGAAGAT TCACCCTTTG GTGGTCCCCA 
ACCATCAATC GGGCCAATGT ATATGTGGGC TTTCAGGTGC AGCTAGACCT GACAGGTATC 
TTCATGCATG GCAAGATCCC CACGTTGAAG ATCTCTCTCA TCCAAATCTT TCGAGCCCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTC ATGGATTTGT GTCAGGTATT TGACCAGGAA 
CTGGATGCAC TAGAAATAGA GACCGTACAA AAGGAGACAA TCCATCCCCG AAAGTCTTAT 
AAGATGAACT CTTCCTGTGC AGATATATTG CTTTTTGCCT CCTATAAGTG GAATGTCTCT 
CGACCCTCAT TGCTAGCAGA TTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATAGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ATGACATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGACTA TACCACAGAC AACATGAGTA TTTACCCTTC GCCTACAGGT 
GTGCTCATTG CAATTGACTT GGCGTATAAT CTGCACAGTG CTTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGAAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA GCCCACTGAG 
CCGTATTTGT CATCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTA 
GATGATACTA ATGTCTACAG AGTAACTATC CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGGA CAGGGCAGCT GTTTCTCAAG 
ATAATTCATA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC CAAGTGGAAG 
ACAGCTGAAG AAGTAGCTGC CCTGATCAGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAA 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCTTTGGAGG TACACTTGCT GGACTTCCCT 
AATATTGTGA TCAAAGGATC GGAGCTTCAA CTCCCCTTCC AGGCTTGTCT AAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TTAAGACAAT CTCATCTTAC ACGGCTTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCTCTGC ATGTGAACAA TGACCGGGCA AAAGTAATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CCCACCACAT CTGGCCCACC CTGACTGACG AAGAGTGGAT CAAAGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCCGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGAGACATA ATCTTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGAC AGCAGATAGC AGAGATCGAG AAGCAGACAA AGGAACAGTC ACAGCTGACT 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGTGATGAGA TCATCACCTC CACAACCAGC 
AACTATGAAA CTCAGACTTT CTCCTCAAAA ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAATCTGC ACCTACGGAC CAATCACATT TATGTTTCTT CTGATGACAT TAAAGAGACT 
GGTTACACTT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT CTCTGACCTT 
CGTGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGCCCAC CGGATAACCC CCAAGTGAAG 
GAAATCCGCT GCATCGTGAT GGTTCCACAG TGGGGTACTC ACCAGACTGT GCACCTGCCT 
GGTCAGCTGC CCCAGCATGA GTACCTCAAA GAGATGGAGC CGTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CATTGACAGC CTACAAGTTG ACACCCAGTG GCTATGAATG GGGCCGCCAG 
AATACAGACA AAGGCAACAA CCCCAAGGGT TATCTACCCT CACACTATGA GAGGGTCCAG 
ATGTTGCTGT CAGACCGATT CCTTGGCTTC TTTATGGTTC CTGCTCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG ACATGATCCC AACATGAAGT ATGAGCTACA GCTGGCAAAC 
CCTAAAGAGT TCTACCATGA GGTGCACAGA CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAAGAGGGAG AGGTCTACTC TGCAGACCGC GAGGACCTAT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021583834.1
CDS	89..7096
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_021728159.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021728159.1

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCAG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGACT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CCAGAAAATG GCAGCAGTTG 
CAGGCCAAAC GGTATGCAGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAT 
ATGCCTCCAG AACATGTCAG GAAGATCATT CGAGACCACG GAGACATGAC CAACAGAAAG 
TTTCGCCATG ACAAAAGAGT CTATCTGGGT GCCCTAAAGT ACATGCCCCA TGCGGTCCTC 
AAACTCCTGG AGAATATGCC TATGCCTTGG GAACAGATCC GGGATGTGCC TGTGCTGTAC 
CATATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCTT GGGTCATTGA GCCAGTCTAT 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTTAAGAGGA TGCGCTTCCC TCCTTTTGAT GATGAGGAGC CCCCCTTGGA CTATGCTGAT 
AACATTTTAG ATGTTGAGCC TCTTGAAGCC ATTCAGCTAG AGTTGGACCC TGAGGAGGAT 
GCACCTGTGT TGGACTGGTT TTATGATCAC CAGCCATTGA GAGACAGTAG AAAGTATGTT 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCTA TGATGTCTAC TCTATACCGC 
CTGGCCAATC AGCTCCTAAC AGATTTGGTG GACGACAATT ACTTCTATCT TTTTGATCTA 
AAGGCCTTCT TTACCTCCAA GGCGCTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGATTGGA ATGAATTTAA TGATATTAAC 
AAAATCATTA TTCGGCAGCC TATCCGCACT GAATATAAGA TTGCTTTTCC TTACCTATAC 
AACAACCTTC CACATCACGT TCATCTCACC TGGTATCATA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA TCCAATCTCT 
CACAGGCATT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ATGATGAAGA ATTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CTTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCTC TCCTTTGGGC CCCACGGCCC TTCAATCTTC GCTCTGGCCG CACCCGCCGG 
GCTCTGGACA TACCCCTTGT CAAGAATTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTCAAAGTGA GAGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCTTGAAA 
CATCGTCCCC CTAAGGCTCA AAAGAAGAGG TATCTGTTCC GCTCCTTCAA AGCTACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAAGTTGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAAAACCTTA ACTACCTGCA CCTGGACTAT 
AACTTCAACC TCAAACCTGT CAAGACACTT ACCACTAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCATTTGTG TCGGGAAGTA CTGCGTTTAA CTAAGCTGGT TGTAGACAGT 
CACGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTTCAGC TGGCAGATGG ATTACAGTAT 
ATATTTGCCC ATGTGGGGCA GCTGACAGGC ATGTATCGTT ACAAATACAA ACTGATGAGA 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGTGTCTG GCTATTTTTC 
ATGCGTGGCA TTACTCCTTT GCTAGAGCGA TGGCTAGGCA ATCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCAAAA ACAGTAACGA AGCAGCGAGT GGAGTCACAT 
TTTGATCTTG AACTTCGGGC AGCTGTGATG CATGACATTC TGGATATGAT GCCTGAGGGG 
ATCAAACAGA ATAAGGCCCG GACAATCCTG CAGCATCTCA GCGAAGCCTG GCGTTGCTGG 
AAGGCCAACA TTCCCTGGAA AGTCCCCGGA CTACCAACAC CCATAGAAAA TATGATCCTT 
CGATATGTGA AAGCCAAGGC TGACTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGCCGTG GGGCCACTGT AGACAAGACT GTTTGCAAAA AGAACTTGGG TCGCCTTACT 
CGATTGTACC TGAAGGCAGA ACAGGAGCGA CAGCACAATT ACCTAAAGGA TGGGCCTTAC 
ATCACGGCAG AGGAAGCAGT GGCAGTGTAT ACCACTACTG TGCATTGGTT GGAAAGCCGG 
AGGTTCTCGC CCATTCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTAATT 
TTGGCTTTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAAGAGT TAGGTCTGAT TGAGCAAGCC TATGACAACC CCCATGAGGC ATTGTCTCGC 
ATCAAGCGTC ACCTCCTTAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA ATTCATGGAT 
CTTTATAGTC ACCTGGTTCC AGTGTATGAT GTGGAACCAC TGGAGAAGAT AACTGATGCC 
TACTTGGACC AGTATCTGTG GTATGAAGCA GACAAGCGCC GCCTTTTCCC ACCCTGGATC 
AAACCTGCTG ATACGGAACC ACCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACTTGCAGG ATGTGTGGGA AACCAGTGAG GGTGAGTGCA ATGTCATGCT GGAATCACGC 
TTTGAGAAAA TGTATGAAAA GATTGATTTG ACCCTGCTCA ATAGGCTGCT GCGACTCATT 
GTGGATCACA ACATAGCTGA CTACATGACA GCTAAGAACA ATGTTGTCAT CAACTATAAG 
GATATGAACC ATACTAATTC ATACGGGATC ATCAGAGGAC TGCAGTTTGC CTCTTTCATT 
GTGCAATACT ATGGTCTGGT GATGGATTTG CTGGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTTAGTT TCCAGGACAT AGCCACTGAA 
GCTGCTCATC CCATCCGACT CTTCTGTAGA TACATTGATC GAATTCATAT TTTCTTCAGG 
TTCACTGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TAACAGAGCA CCCTGACCCC 
AACAATGAAA ACATCGTTGG CTATAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGCCTCATGA AGCATGATGT CAATTTAGGT CGAGCAGTAT TTTGGGACAT CAAGAACCGT 
CTGCCACGAT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCTGT GTATAGTAAA 
GACAACCCTA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAATGC 
CGCACCAGCT ATGAGGAGTT TACACACAAG GATGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAAC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGATTC 
CACAACCGTG TACGTCAAAT TCTGATGGCT TCTGGCTCTA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACTCAGGAAC TCCTAGACTT GCTTGTGAAG TGTGAGAACA AGATCCAGAC ACGTATAAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCTCCTG TTGTATTCTA CACCCCTAAG 
GAGTTGGGAG GACTGGGCAT GCTGTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATT ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTATACCGC TACATACAGC CATGGGAGAG TGAGTTCATC 
GATTCTCAGC GGGTCTGGGC TGAATATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGGCTGA CATTAGAAGA TCTAGAGGAC TCTTGGGATC GTGGCATTCC TCGAATCAAT 
ACTCTCTTCC AGAAGGATCG GCACACATTA GCTTATGATA AGGGTTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAA AACCCTTTTT GGTGGACACA CCAGCGACAT 
GATGGGAAGC TCTGGAACCT AAACAATTAC CGTACAGATA TGATTCAAGC CCTGGGTGGT 
GTAGAAGGCA TTCTGGAACA CACACTGTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTTTTCTGGG AGAAGGCTAG TGGCTTTGAA GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGAAGAT TCACCCTTTG GTGGTCCCCA 
ACCATCAATC GGGCCAATGT ATATGTGGGC TTTCAGGTGC AGCTAGACCT GACAGGTATC 
TTCATGCATG GCAAGATCCC CACGTTGAAG ATCTCTCTCA TCCAAATCTT TCGAGCCCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTC ATGGATTTGT GTCAGGTATT TGACCAGGAA 
CTGGATGCAC TAGAAATAGA GACCGTACAA AAGGAGACAA TCCATCCCCG AAAGTCTTAT 
AAGATGAACT CTTCCTGTGC AGATATATTG CTTTTTGCCT CCTATAAGTG GAATGTCTCT 
CGACCCTCAT TGCTAGCAGA TTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATAGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ATGACATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGACTA TACCACAGAC AACATGAGTA TTTACCCTTC GCCTACAGGT 
GTGCTCATTG CAATTGACTT GGCGTATAAT CTGCACAGTG CTTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGAAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA GCCCACTGAG 
CCGTATTTGT CATCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTA 
GATGATACTA ATGTCTACAG AGTAACTATC CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGGA CAGGGCAGCT GTTTCTCAAG 
ATAATTCATA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC CAAGTGGAAG 
ACAGCTGAAG AAGTAGCTGC CCTGATCAGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAA 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCTTTGGAGG TACACTTGCT GGACTTCCCT 
AATATTGTGA TCAAAGGATC GGAGCTTCAA CTCCCCTTCC AGGCTTGTCT AAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TTAAGACAAT CTCATCTTAC ACGGCTTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCTCTGC ATGTGAACAA TGACCGGGCA AAAGTAATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CCCACCACAT CTGGCCCACC CTGACTGACG AAGAGTGGAT CAAAGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCCGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGAGACATA ATCTTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGAC AGCAGATAGC AGAGATCGAG AAGCAGACAA AGGAACAGTC ACAGCTGACT 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGTGATGAGA TCATCACCTC CACAACCAGC 
AACTATGAAA CTCAGACTTT CTCCTCAAAA ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAATCTGC ACCTACGGAC CAATCACATT TATGTTTCTT CTGATGACAT TAAAGAGACT 
GGTTACACTT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT CTCTGACCTT 
CGTGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGCCCAC CGGATAACCC CCAAGTGAAG 
GAAATCCGCT GCATCGTGAT GGTTCCACAG TGGGGTACTC ACCAGACTGT GCACCTGCCT 
GGTCAGCTGC CCCAGCATGA GTACCTCAAA GAGATGGAGC CGTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CATTGACAGC CTACAAGTTG ACACCCAGTG GCTATGAATG GGGCCGCCAG 
AATACAGACA AAGGCAACAA CCCCAAGGGT TATCTACCCT CACACTATGA GAGGGTCCAG 
ATGTTGCTGT CAGACCGATT CCTTGGCTTC TTTATGGTTC CTGCTCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG ACATGATCCC AACATGAAGT ATGAGCTACA GCTGGCAAAC 
CCTAAAGAGT TCTACCATGA GGTGCACAGA CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAAGAGGGAG AGGTCTACTC TGCAGACCGC GAGGACCTAT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prpf8 ( XM_005327904.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005327961.1 Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.

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CloneID	OIc58916
Clone ID Related Accession (Same CDS sequence)	XM_021728159.1 , XM_005327904.2
Accession Version	XM_005327904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005327904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCAG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGACT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CCAGAAAATG GCAGCAGTTG 
CAGGCCAAAC GGTATGCAGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAT 
ATGCCTCCAG AACATGTCAG GAAGATCATT CGAGACCACG GAGACATGAC CAACAGAAAG 
TTTCGCCATG ACAAAAGAGT CTATCTGGGT GCCCTAAAGT ACATGCCCCA TGCGGTCCTC 
AAACTCCTGG AGAATATGCC TATGCCTTGG GAACAGATCC GGGATGTGCC TGTGCTGTAC 
CATATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCTT GGGTCATTGA GCCAGTCTAT 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTTAAGAGGA TGCGCTTCCC TCCTTTTGAT GATGAGGAGC CCCCCTTGGA CTATGCTGAT 
AACATTTTAG ATGTTGAGCC TCTTGAAGCC ATTCAGCTAG AGTTGGACCC TGAGGAGGAT 
GCACCTGTGT TGGACTGGTT TTATGATCAC CAGCCATTGA GAGACAGTAG AAAGTATGTT 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCTA TGATGTCTAC TCTATACCGC 
CTGGCCAATC AGCTCCTAAC AGATTTGGTG GACGACAATT ACTTCTATCT TTTTGATCTA 
AAGGCCTTCT TTACCTCCAA GGCGCTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGATTGGA ATGAATTTAA TGATATTAAC 
AAAATCATTA TTCGGCAGCC TATCCGCACT GAATATAAGA TTGCTTTTCC TTACCTATAC 
AACAACCTTC CACATCACGT TCATCTCACC TGGTATCATA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA TCCAATCTCT 
CACAGGCATT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ATGATGAAGA ATTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CTTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCTC TCCTTTGGGC CCCACGGCCC TTCAATCTTC GCTCTGGCCG CACCCGCCGG 
GCTCTGGACA TACCCCTTGT CAAGAATTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTCAAAGTGA GAGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCTTGAAA 
CATCGTCCCC CTAAGGCTCA AAAGAAGAGG TATCTGTTCC GCTCCTTCAA AGCTACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAAGTTGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAAAACCTTA ACTACCTGCA CCTGGACTAT 
AACTTCAACC TCAAACCTGT CAAGACACTT ACCACTAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCATTTGTG TCGGGAAGTA CTGCGTTTAA CTAAGCTGGT TGTAGACAGT 
CACGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTTCAGC TGGCAGATGG ATTACAGTAT 
ATATTTGCCC ATGTGGGGCA GCTGACAGGC ATGTATCGTT ACAAATACAA ACTGATGAGA 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGTGTCTG GCTATTTTTC 
ATGCGTGGCA TTACTCCTTT GCTAGAGCGA TGGCTAGGCA ATCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCAAAA ACAGTAACGA AGCAGCGAGT GGAGTCACAT 
TTTGATCTTG AACTTCGGGC AGCTGTGATG CATGACATTC TGGATATGAT GCCTGAGGGG 
ATCAAACAGA ATAAGGCCCG GACAATCCTG CAGCATCTCA GCGAAGCCTG GCGTTGCTGG 
AAGGCCAACA TTCCCTGGAA AGTCCCCGGA CTACCAACAC CCATAGAAAA TATGATCCTT 
CGATATGTGA AAGCCAAGGC TGACTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGCCGTG GGGCCACTGT AGACAAGACT GTTTGCAAAA AGAACTTGGG TCGCCTTACT 
CGATTGTACC TGAAGGCAGA ACAGGAGCGA CAGCACAATT ACCTAAAGGA TGGGCCTTAC 
ATCACGGCAG AGGAAGCAGT GGCAGTGTAT ACCACTACTG TGCATTGGTT GGAAAGCCGG 
AGGTTCTCGC CCATTCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTAATT 
TTGGCTTTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAAGAGT TAGGTCTGAT TGAGCAAGCC TATGACAACC CCCATGAGGC ATTGTCTCGC 
ATCAAGCGTC ACCTCCTTAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA ATTCATGGAT 
CTTTATAGTC ACCTGGTTCC AGTGTATGAT GTGGAACCAC TGGAGAAGAT AACTGATGCC 
TACTTGGACC AGTATCTGTG GTATGAAGCA GACAAGCGCC GCCTTTTCCC ACCCTGGATC 
AAACCTGCTG ATACGGAACC ACCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACTTGCAGG ATGTGTGGGA AACCAGTGAG GGTGAGTGCA ATGTCATGCT GGAATCACGC 
TTTGAGAAAA TGTATGAAAA GATTGATTTG ACCCTGCTCA ATAGGCTGCT GCGACTCATT 
GTGGATCACA ACATAGCTGA CTACATGACA GCTAAGAACA ATGTTGTCAT CAACTATAAG 
GATATGAACC ATACTAATTC ATACGGGATC ATCAGAGGAC TGCAGTTTGC CTCTTTCATT 
GTGCAATACT ATGGTCTGGT GATGGATTTG CTGGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTTAGTT TCCAGGACAT AGCCACTGAA 
GCTGCTCATC CCATCCGACT CTTCTGTAGA TACATTGATC GAATTCATAT TTTCTTCAGG 
TTCACTGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TAACAGAGCA CCCTGACCCC 
AACAATGAAA ACATCGTTGG CTATAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGCCTCATGA AGCATGATGT CAATTTAGGT CGAGCAGTAT TTTGGGACAT CAAGAACCGT 
CTGCCACGAT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCTGT GTATAGTAAA 
GACAACCCTA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAATGC 
CGCACCAGCT ATGAGGAGTT TACACACAAG GATGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAAC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGATTC 
CACAACCGTG TACGTCAAAT TCTGATGGCT TCTGGCTCTA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACTCAGGAAC TCCTAGACTT GCTTGTGAAG TGTGAGAACA AGATCCAGAC ACGTATAAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCTCCTG TTGTATTCTA CACCCCTAAG 
GAGTTGGGAG GACTGGGCAT GCTGTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATT ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTATACCGC TACATACAGC CATGGGAGAG TGAGTTCATC 
GATTCTCAGC GGGTCTGGGC TGAATATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGGCTGA CATTAGAAGA TCTAGAGGAC TCTTGGGATC GTGGCATTCC TCGAATCAAT 
ACTCTCTTCC AGAAGGATCG GCACACATTA GCTTATGATA AGGGTTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAA AACCCTTTTT GGTGGACACA CCAGCGACAT 
GATGGGAAGC TCTGGAACCT AAACAATTAC CGTACAGATA TGATTCAAGC CCTGGGTGGT 
GTAGAAGGCA TTCTGGAACA CACACTGTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTTTTCTGGG AGAAGGCTAG TGGCTTTGAA GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGAAGAT TCACCCTTTG GTGGTCCCCA 
ACCATCAATC GGGCCAATGT ATATGTGGGC TTTCAGGTGC AGCTAGACCT GACAGGTATC 
TTCATGCATG GCAAGATCCC CACGTTGAAG ATCTCTCTCA TCCAAATCTT TCGAGCCCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTC ATGGATTTGT GTCAGGTATT TGACCAGGAA 
CTGGATGCAC TAGAAATAGA GACCGTACAA AAGGAGACAA TCCATCCCCG AAAGTCTTAT 
AAGATGAACT CTTCCTGTGC AGATATATTG CTTTTTGCCT CCTATAAGTG GAATGTCTCT 
CGACCCTCAT TGCTAGCAGA TTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATAGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ATGACATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGACTA TACCACAGAC AACATGAGTA TTTACCCTTC GCCTACAGGT 
GTGCTCATTG CAATTGACTT GGCGTATAAT CTGCACAGTG CTTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGAAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA GCCCACTGAG 
CCGTATTTGT CATCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTA 
GATGATACTA ATGTCTACAG AGTAACTATC CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGGA CAGGGCAGCT GTTTCTCAAG 
ATAATTCATA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC CAAGTGGAAG 
ACAGCTGAAG AAGTAGCTGC CCTGATCAGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAA 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCTTTGGAGG TACACTTGCT GGACTTCCCT 
AATATTGTGA TCAAAGGATC GGAGCTTCAA CTCCCCTTCC AGGCTTGTCT AAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TTAAGACAAT CTCATCTTAC ACGGCTTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCTCTGC ATGTGAACAA TGACCGGGCA AAAGTAATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CCCACCACAT CTGGCCCACC CTGACTGACG AAGAGTGGAT CAAAGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCCGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGAGACATA ATCTTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGAC AGCAGATAGC AGAGATCGAG AAGCAGACAA AGGAACAGTC ACAGCTGACT 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGTGATGAGA TCATCACCTC CACAACCAGC 
AACTATGAAA CTCAGACTTT CTCCTCAAAA ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAATCTGC ACCTACGGAC CAATCACATT TATGTTTCTT CTGATGACAT TAAAGAGACT 
GGTTACACTT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT CTCTGACCTT 
CGTGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGCCCAC CGGATAACCC CCAAGTGAAG 
GAAATCCGCT GCATCGTGAT GGTTCCACAG TGGGGTACTC ACCAGACTGT GCACCTGCCT 
GGTCAGCTGC CCCAGCATGA GTACCTCAAA GAGATGGAGC CGTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CATTGACAGC CTACAAGTTG ACACCCAGTG GCTATGAATG GGGCCGCCAG 
AATACAGACA AAGGCAACAA CCCCAAGGGT TATCTACCCT CACACTATGA GAGGGTCCAG 
ATGTTGCTGT CAGACCGATT CCTTGGCTTC TTTATGGTTC CTGCTCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG ACATGATCCC AACATGAAGT ATGAGCTACA GCTGGCAAAC 
CCTAAAGAGT TCTACCATGA GGTGCACAGA CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAAGAGGGAG AGGTCTACTC TGCAGACCGC GAGGACCTAT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005327961.1
CDS	112..7119
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_005327904.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus pre-mRNA processing factor 8 (Prpf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005327904.2

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCAG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGACT ACATGTCAGA GGAGAAGCTG CAGGAGAAAG CCAGAAAATG GCAGCAGTTG 
CAGGCCAAAC GGTATGCAGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAT 
ATGCCTCCAG AACATGTCAG GAAGATCATT CGAGACCACG GAGACATGAC CAACAGAAAG 
TTTCGCCATG ACAAAAGAGT CTATCTGGGT GCCCTAAAGT ACATGCCCCA TGCGGTCCTC 
AAACTCCTGG AGAATATGCC TATGCCTTGG GAACAGATCC GGGATGTGCC TGTGCTGTAC 
CATATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCTT GGGTCATTGA GCCAGTCTAT 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTTAAGAGGA TGCGCTTCCC TCCTTTTGAT GATGAGGAGC CCCCCTTGGA CTATGCTGAT 
AACATTTTAG ATGTTGAGCC TCTTGAAGCC ATTCAGCTAG AGTTGGACCC TGAGGAGGAT 
GCACCTGTGT TGGACTGGTT TTATGATCAC CAGCCATTGA GAGACAGTAG AAAGTATGTT 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCTA TGATGTCTAC TCTATACCGC 
CTGGCCAATC AGCTCCTAAC AGATTTGGTG GACGACAATT ACTTCTATCT TTTTGATCTA 
AAGGCCTTCT TTACCTCCAA GGCGCTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGATTGGA ATGAATTTAA TGATATTAAC 
AAAATCATTA TTCGGCAGCC TATCCGCACT GAATATAAGA TTGCTTTTCC TTACCTATAC 
AACAACCTTC CACATCACGT TCATCTCACC TGGTATCATA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA TCCAATCTCT 
CACAGGCATT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ATGATGAAGA ATTTGAGCTC 
CCAGAGTTTG TGGAGCCCTT CTTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCTC TCCTTTGGGC CCCACGGCCC TTCAATCTTC GCTCTGGCCG CACCCGCCGG 
GCTCTGGACA TACCCCTTGT CAAGAATTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTCAAAGTGA GAGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCTTGAAA 
CATCGTCCCC CTAAGGCTCA AAAGAAGAGG TATCTGTTCC GCTCCTTCAA AGCTACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAAGTTGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGT AAAAACCTTA ACTACCTGCA CCTGGACTAT 
AACTTCAACC TCAAACCTGT CAAGACACTT ACCACTAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCATTTGTG TCGGGAAGTA CTGCGTTTAA CTAAGCTGGT TGTAGACAGT 
CACGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTTCAGC TGGCAGATGG ATTACAGTAT 
ATATTTGCCC ATGTGGGGCA GCTGACAGGC ATGTATCGTT ACAAATACAA ACTGATGAGA 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTGGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGTGTCTG GCTATTTTTC 
ATGCGTGGCA TTACTCCTTT GCTAGAGCGA TGGCTAGGCA ATCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCAAAA ACAGTAACGA AGCAGCGAGT GGAGTCACAT 
TTTGATCTTG AACTTCGGGC AGCTGTGATG CATGACATTC TGGATATGAT GCCTGAGGGG 
ATCAAACAGA ATAAGGCCCG GACAATCCTG CAGCATCTCA GCGAAGCCTG GCGTTGCTGG 
AAGGCCAACA TTCCCTGGAA AGTCCCCGGA CTACCAACAC CCATAGAAAA TATGATCCTT 
CGATATGTGA AAGCCAAGGC TGACTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGCCGTG GGGCCACTGT AGACAAGACT GTTTGCAAAA AGAACTTGGG TCGCCTTACT 
CGATTGTACC TGAAGGCAGA ACAGGAGCGA CAGCACAATT ACCTAAAGGA TGGGCCTTAC 
ATCACGGCAG AGGAAGCAGT GGCAGTGTAT ACCACTACTG TGCATTGGTT GGAAAGCCGG 
AGGTTCTCGC CCATTCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTAATT 
TTGGCTTTGG AGCGACTCAA GGAAGCTTAT AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAAGAGT TAGGTCTGAT TGAGCAAGCC TATGACAACC CCCATGAGGC ATTGTCTCGC 
ATCAAGCGTC ACCTCCTTAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA ATTCATGGAT 
CTTTATAGTC ACCTGGTTCC AGTGTATGAT GTGGAACCAC TGGAGAAGAT AACTGATGCC 
TACTTGGACC AGTATCTGTG GTATGAAGCA GACAAGCGCC GCCTTTTCCC ACCCTGGATC 
AAACCTGCTG ATACGGAACC ACCTCCACTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACTTGCAGG ATGTGTGGGA AACCAGTGAG GGTGAGTGCA ATGTCATGCT GGAATCACGC 
TTTGAGAAAA TGTATGAAAA GATTGATTTG ACCCTGCTCA ATAGGCTGCT GCGACTCATT 
GTGGATCACA ACATAGCTGA CTACATGACA GCTAAGAACA ATGTTGTCAT CAACTATAAG 
GATATGAACC ATACTAATTC ATACGGGATC ATCAGAGGAC TGCAGTTTGC CTCTTTCATT 
GTGCAATACT ATGGTCTGGT GATGGATTTG CTGGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTTAGTT TCCAGGACAT AGCCACTGAA 
GCTGCTCATC CCATCCGACT CTTCTGTAGA TACATTGATC GAATTCATAT TTTCTTCAGG 
TTCACTGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TAACAGAGCA CCCTGACCCC 
AACAATGAAA ACATCGTTGG CTATAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGTATG 
CGCCTCATGA AGCATGATGT CAATTTAGGT CGAGCAGTAT TTTGGGACAT CAAGAACCGT 
CTGCCACGAT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTATCTGT GTATAGTAAA 
GACAACCCTA ACCTGCTGTT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAATGC 
CGCACCAGCT ATGAGGAGTT TACACACAAG GATGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAAC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGATTC 
CACAACCGTG TACGTCAAAT TCTGATGGCT TCTGGCTCTA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACTCAGGAAC TCCTAGACTT GCTTGTGAAG TGTGAGAACA AGATCCAGAC ACGTATAAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCTCCTG TTGTATTCTA CACCCCTAAG 
GAGTTGGGAG GACTGGGCAT GCTGTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATT ACACACTTTC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTATACCGC TACATACAGC CATGGGAGAG TGAGTTCATC 
GATTCTCAGC GGGTCTGGGC TGAATATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGGCTGA CATTAGAAGA TCTAGAGGAC TCTTGGGATC GTGGCATTCC TCGAATCAAT 
ACTCTCTTCC AGAAGGATCG GCACACATTA GCTTATGATA AGGGTTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAA AACCCTTTTT GGTGGACACA CCAGCGACAT 
GATGGGAAGC TCTGGAACCT AAACAATTAC CGTACAGATA TGATTCAAGC CCTGGGTGGT 
GTAGAAGGCA TTCTGGAACA CACACTGTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTTTTCTGGG AGAAGGCTAG TGGCTTTGAA GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGATTGAA CCAGATTCCC AATCGAAGAT TCACCCTTTG GTGGTCCCCA 
ACCATCAATC GGGCCAATGT ATATGTGGGC TTTCAGGTGC AGCTAGACCT GACAGGTATC 
TTCATGCATG GCAAGATCCC CACGTTGAAG ATCTCTCTCA TCCAAATCTT TCGAGCCCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTC ATGGATTTGT GTCAGGTATT TGACCAGGAA 
CTGGATGCAC TAGAAATAGA GACCGTACAA AAGGAGACAA TCCATCCCCG AAAGTCTTAT 
AAGATGAACT CTTCCTGTGC AGATATATTG CTTTTTGCCT CCTATAAGTG GAATGTCTCT 
CGACCCTCAT TGCTAGCAGA TTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATAGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ATGACATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGACTA TACCACAGAC AACATGAGTA TTTACCCTTC GCCTACAGGT 
GTGCTCATTG CAATTGACTT GGCGTATAAT CTGCACAGTG CTTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGAAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA GCCCACTGAG 
CCGTATTTGT CATCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTA 
GATGATACTA ATGTCTACAG AGTAACTATC CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGGA CAGGGCAGCT GTTTCTCAAG 
ATAATTCATA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GGCAGTTGGC CAAGTGGAAG 
ACAGCTGAAG AAGTAGCTGC CCTGATCAGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAA 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCTTTGGAGG TACACTTGCT GGACTTCCCT 
AATATTGTGA TCAAAGGATC GGAGCTTCAA CTCCCCTTCC AGGCTTGTCT AAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GATGACTGGC TTAAGACAAT CTCATCTTAC ACGGCTTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCTCTGC ATGTGAACAA TGACCGGGCA AAAGTAATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CCCACCACAT CTGGCCCACC CTGACTGACG AAGAGTGGAT CAAAGTGGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCCGAC TATGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AGTCAGAAAT TCGAGACATA ATCTTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGAC AGCAGATAGC AGAGATCGAG AAGCAGACAA AGGAACAGTC ACAGCTGACT 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGTGATGAGA TCATCACCTC CACAACCAGC 
AACTATGAAA CTCAGACTTT CTCCTCAAAA ACTGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAATCTGC ACCTACGGAC CAATCACATT TATGTTTCTT CTGATGACAT TAAAGAGACT 
GGTTACACTT ATATCCTTCC CAAGAATGTG CTTAAGAAGT TTATCTGCAT CTCTGACCTT 
CGTGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGCCCAC CGGATAACCC CCAAGTGAAG 
GAAATCCGCT GCATCGTGAT GGTTCCACAG TGGGGTACTC ACCAGACTGT GCACCTGCCT 
GGTCAGCTGC CCCAGCATGA GTACCTCAAA GAGATGGAGC CGTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCCCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGTTCCTGCA CATTGACAGC CTACAAGTTG ACACCCAGTG GCTATGAATG GGGCCGCCAG 
AATACAGACA AAGGCAACAA CCCCAAGGGT TATCTACCCT CACACTATGA GAGGGTCCAG 
ATGTTGCTGT CAGACCGATT CCTTGGCTTC TTTATGGTTC CTGCTCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG ACATGATCCC AACATGAAGT ATGAGCTACA GCTGGCAAAC 
CCTAAAGAGT TCTACCATGA GGTGCACAGA CCCTCCCACT TCCTCAACTT TGCCCTCCTG 
CAAGAGGGAG AGGTCTACTC TGCAGACCGC GAGGACCTAT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Prpf8 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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