HECW1 cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	101954301
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005319222.3	XP_005319279.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_021722299.1	XP_021577974.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_005319222.2	XP_005319279.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_021722298.1	XP_021577973.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_021722300.1	XP_021577975.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_005319221.1	XP_005319278.1	E3 ubiquitin-protein ligase HECW1 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc50425	XM_005319221.1 Latest version!	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc50425	XM_021722299.1 Latest version!	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc50426	XM_005319222.3 Latest version!	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc75360	XM_021722298.1 Latest version!	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIc75361	XM_021722300.1 Latest version!	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OIc50426	XM_005319222.2	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_021722299.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_021577974.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc50425
Clone ID Related Accession (Same CDS sequence)	XM_021722299.1 , XM_005319221.1
Accession Version	XM_021722299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAGTAAGCCA GCTTCCTTCC 
TTGAGACCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAA 
GCTCGAATTG ACAGCCATGG GCGGGTCTTT TATGTGGACC ATGTGAACCG TACCACCACC 
TGGCAGCGTC CAACAGCAGC AGCCACCCCA GACGGGATGC GGAGATCCGG GTCCATCCAG 
CAGATGGAGC AGCTCAACAG GCGGTATCAA AATATTCAGC GGACCATTGC AACAGAAAGA 
TCTGAAGAGG ATTCTGGCAG CCAAAGCTGT GAGCAAGTCC CAGCCGGAGG AGGTGGAGGT 
GGAGGGAGTG ACTCAGAGGC TGAATCTCCC CAATCAAGCT TAGATCAGAG GAGAGAAGGG 
TCACTTTCTC CAGTTAACTC ACAAAAAATC ACCTTGCTGC TGCAGTCCCC AGCCGTCAAG 
TTCATCACCA ACCCAGAATT CTTCACTGTG CTGCATGCCA ATTATAGTGC CTACCGAGTC 
TTCACCAGTA GCACCTGCTT AAAGCACATG ATTCTGAAAG TCCGAAGAGA CGCTCGCAAT 
TTTGAACGCT ACCAGCACAA CCGGGACTTG GTGAATTTCA TCAACATGTT CGCAGACACT 
CGGCTGGAAC TGCCCCGAGG CTGGGAGATC AAAACAGACC AGCAGGGGAA GTCTTTTTTT 
GTGGACCACA ACAGTCGAGC TACCACTTTC ATTGACCCCC GAATCCCTCT TCAGAATGGT 
CGTCTTCCCA ATCATCTGAC TCATCGACAG CACCTCCAGA GGCTCCGAAG TTACAGCGCT 
GGAGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCCTCCT TACTGGCCAG GCCAGGACAC 
AGCCTAGTAG CTGCTATTCG AAGCCAACAC CAACATGAAG CATTGCCACT GGCATATAAT 
GACAAGATCG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAGCGTCAG 
CCAAGCTTGG CAAGAAATCA CACACTCAGG GAGAAAATCC ATTATATTCG GACCGAGGGT 
AATCATGGGC TTGAGAAGTT GTCCTGTGAT GCAGATCTAG TTATTTTGCT GAGCCTCTTT 
GAAGAAGAGA TTATGTCCTA TGTACCCCTG CAGGCTGCCT TCCACCCCGG GTATAGCTTT 
TCTCCCCGCT GTTCACCCTG TTCTTCACCT CAGAACTCTC CAGGTTTACA GAGAGCCAGT 
GCAAGAGCCC CTTCACCATA TCGGAGAGAC TTTGAGGCCA AGCTCCGCAA TTTCTATCGG 
AAACTAGAAG CCAAAGGATT TGGTCAGGGT CCAGGGAAAA TTAAGCTCAT TATTCGTCGG 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCCT ATTCCCGGAA GGAGCTCCAG 
AGAAACAAGC TCTACGTCAC CTTTGTTGGA GAGGAGGGCC TGGACTACAG TGGTCCTTCT 
CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG CTCTTCAACC CTTACTACGG ACTCTTTGAG 
TACTCTGCAA ATGATACTTA CACGGTGCAG ATCAGCCCCA TGTCTGCGTT TGTAGAAAAC 
CACCTGGAAT GGTTCAGGTT TAGTGGTCGT ATCCTAGGCC TGGCTTTGAT CCATCAGTAC 
CTTCTGGATG CTTTCTTCAC AAGGCCATTC TATAAGGCAC TCCTGAGGCT GCCCTGTGAT 
TTGAGTGATC TGGAATATTT GGACGAGGAA TTCCACCAGA GCTTGCAGTG GATGAAGGAC 
AACAACATCA CAGATATCCT CGACCTCACT TTCACTGTTA ATGAAGAGGT TTTTGGACAG 
GTAACAGAAA GGGAGTTAAA ATCTGGAGGA GCCAACACCC AGGTGACGGA GAAGAACAAG 
AAGGAGTACA TCGAGAGGAT GGTCAAGTGG CGGGTGGAGC GTGGCGTGGT GCAGCAGACT 
GAGGCACTGG TGCGGGGCTT CTACGAGGTT GTAGACTCGA GGCTCGTCTC AGTGTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATAGCTGGC ACCGCAGAGA TCGACCTGAA TGACTGGCGG 
AATAACACTG AGTATCGGGG AGGTTACCAT GATGGGCATC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AGCGCTTCAA TAATGAGCAG AGATTAAGAT TACTTCAGTT TGTCACGGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCT GCCCTTCGTG GAAGCAATGG GCTTCGGCGT 
TTCTGCATAG AGAAATGGGG GAAAATTACT TCTCTTCCCA GGGCACACAC GTGCTTCAAC 
CGATTGGATC TTCCACCATA TCCCTCATAC ACTATGTTGT ACGAAAAGCT TTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021577974.1
CDS	80..4912
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021722299.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722299.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAGTAAGCCA GCTTCCTTCC 
TTGAGACCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAA 
GCTCGAATTG ACAGCCATGG GCGGGTCTTT TATGTGGACC ATGTGAACCG TACCACCACC 
TGGCAGCGTC CAACAGCAGC AGCCACCCCA GACGGGATGC GGAGATCCGG GTCCATCCAG 
CAGATGGAGC AGCTCAACAG GCGGTATCAA AATATTCAGC GGACCATTGC AACAGAAAGA 
TCTGAAGAGG ATTCTGGCAG CCAAAGCTGT GAGCAAGTCC CAGCCGGAGG AGGTGGAGGT 
GGAGGGAGTG ACTCAGAGGC TGAATCTCCC CAATCAAGCT TAGATCAGAG GAGAGAAGGG 
TCACTTTCTC CAGTTAACTC ACAAAAAATC ACCTTGCTGC TGCAGTCCCC AGCCGTCAAG 
TTCATCACCA ACCCAGAATT CTTCACTGTG CTGCATGCCA ATTATAGTGC CTACCGAGTC 
TTCACCAGTA GCACCTGCTT AAAGCACATG ATTCTGAAAG TCCGAAGAGA CGCTCGCAAT 
TTTGAACGCT ACCAGCACAA CCGGGACTTG GTGAATTTCA TCAACATGTT CGCAGACACT 
CGGCTGGAAC TGCCCCGAGG CTGGGAGATC AAAACAGACC AGCAGGGGAA GTCTTTTTTT 
GTGGACCACA ACAGTCGAGC TACCACTTTC ATTGACCCCC GAATCCCTCT TCAGAATGGT 
CGTCTTCCCA ATCATCTGAC TCATCGACAG CACCTCCAGA GGCTCCGAAG TTACAGCGCT 
GGAGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCCTCCT TACTGGCCAG GCCAGGACAC 
AGCCTAGTAG CTGCTATTCG AAGCCAACAC CAACATGAAG CATTGCCACT GGCATATAAT 
GACAAGATCG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAGCGTCAG 
CCAAGCTTGG CAAGAAATCA CACACTCAGG GAGAAAATCC ATTATATTCG GACCGAGGGT 
AATCATGGGC TTGAGAAGTT GTCCTGTGAT GCAGATCTAG TTATTTTGCT GAGCCTCTTT 
GAAGAAGAGA TTATGTCCTA TGTACCCCTG CAGGCTGCCT TCCACCCCGG GTATAGCTTT 
TCTCCCCGCT GTTCACCCTG TTCTTCACCT CAGAACTCTC CAGGTTTACA GAGAGCCAGT 
GCAAGAGCCC CTTCACCATA TCGGAGAGAC TTTGAGGCCA AGCTCCGCAA TTTCTATCGG 
AAACTAGAAG CCAAAGGATT TGGTCAGGGT CCAGGGAAAA TTAAGCTCAT TATTCGTCGG 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCCT ATTCCCGGAA GGAGCTCCAG 
AGAAACAAGC TCTACGTCAC CTTTGTTGGA GAGGAGGGCC TGGACTACAG TGGTCCTTCT 
CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG CTCTTCAACC CTTACTACGG ACTCTTTGAG 
TACTCTGCAA ATGATACTTA CACGGTGCAG ATCAGCCCCA TGTCTGCGTT TGTAGAAAAC 
CACCTGGAAT GGTTCAGGTT TAGTGGTCGT ATCCTAGGCC TGGCTTTGAT CCATCAGTAC 
CTTCTGGATG CTTTCTTCAC AAGGCCATTC TATAAGGCAC TCCTGAGGCT GCCCTGTGAT 
TTGAGTGATC TGGAATATTT GGACGAGGAA TTCCACCAGA GCTTGCAGTG GATGAAGGAC 
AACAACATCA CAGATATCCT CGACCTCACT TTCACTGTTA ATGAAGAGGT TTTTGGACAG 
GTAACAGAAA GGGAGTTAAA ATCTGGAGGA GCCAACACCC AGGTGACGGA GAAGAACAAG 
AAGGAGTACA TCGAGAGGAT GGTCAAGTGG CGGGTGGAGC GTGGCGTGGT GCAGCAGACT 
GAGGCACTGG TGCGGGGCTT CTACGAGGTT GTAGACTCGA GGCTCGTCTC AGTGTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATAGCTGGC ACCGCAGAGA TCGACCTGAA TGACTGGCGG 
AATAACACTG AGTATCGGGG AGGTTACCAT GATGGGCATC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AGCGCTTCAA TAATGAGCAG AGATTAAGAT TACTTCAGTT TGTCACGGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCT GCCCTTCGTG GAAGCAATGG GCTTCGGCGT 
TTCTGCATAG AGAAATGGGG GAAAATTACT TCTCTTCCCA GGGCACACAC GTGCTTCAAC 
CGATTGGATC TTCCACCATA TCCCTCATAC ACTATGTTGT ACGAAAAGCT TTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_005319221.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_005319278.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc50425
Clone ID Related Accession (Same CDS sequence)	XM_021722299.1 , XM_005319221.1
Accession Version	XM_005319221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAGTAAGCCA GCTTCCTTCC 
TTGAGACCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAA 
GCTCGAATTG ACAGCCATGG GCGGGTCTTT TATGTGGACC ATGTGAACCG TACCACCACC 
TGGCAGCGTC CAACAGCAGC AGCCACCCCA GACGGGATGC GGAGATCCGG GTCCATCCAG 
CAGATGGAGC AGCTCAACAG GCGGTATCAA AATATTCAGC GGACCATTGC AACAGAAAGA 
TCTGAAGAGG ATTCTGGCAG CCAAAGCTGT GAGCAAGTCC CAGCCGGAGG AGGTGGAGGT 
GGAGGGAGTG ACTCAGAGGC TGAATCTCCC CAATCAAGCT TAGATCAGAG GAGAGAAGGG 
TCACTTTCTC CAGTTAACTC ACAAAAAATC ACCTTGCTGC TGCAGTCCCC AGCCGTCAAG 
TTCATCACCA ACCCAGAATT CTTCACTGTG CTGCATGCCA ATTATAGTGC CTACCGAGTC 
TTCACCAGTA GCACCTGCTT AAAGCACATG ATTCTGAAAG TCCGAAGAGA CGCTCGCAAT 
TTTGAACGCT ACCAGCACAA CCGGGACTTG GTGAATTTCA TCAACATGTT CGCAGACACT 
CGGCTGGAAC TGCCCCGAGG CTGGGAGATC AAAACAGACC AGCAGGGGAA GTCTTTTTTT 
GTGGACCACA ACAGTCGAGC TACCACTTTC ATTGACCCCC GAATCCCTCT TCAGAATGGT 
CGTCTTCCCA ATCATCTGAC TCATCGACAG CACCTCCAGA GGCTCCGAAG TTACAGCGCT 
GGAGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCCTCCT TACTGGCCAG GCCAGGACAC 
AGCCTAGTAG CTGCTATTCG AAGCCAACAC CAACATGAAG CATTGCCACT GGCATATAAT 
GACAAGATCG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAGCGTCAG 
CCAAGCTTGG CAAGAAATCA CACACTCAGG GAGAAAATCC ATTATATTCG GACCGAGGGT 
AATCATGGGC TTGAGAAGTT GTCCTGTGAT GCAGATCTAG TTATTTTGCT GAGCCTCTTT 
GAAGAAGAGA TTATGTCCTA TGTACCCCTG CAGGCTGCCT TCCACCCCGG GTATAGCTTT 
TCTCCCCGCT GTTCACCCTG TTCTTCACCT CAGAACTCTC CAGGTTTACA GAGAGCCAGT 
GCAAGAGCCC CTTCACCATA TCGGAGAGAC TTTGAGGCCA AGCTCCGCAA TTTCTATCGG 
AAACTAGAAG CCAAAGGATT TGGTCAGGGT CCAGGGAAAA TTAAGCTCAT TATTCGTCGG 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCCT ATTCCCGGAA GGAGCTCCAG 
AGAAACAAGC TCTACGTCAC CTTTGTTGGA GAGGAGGGCC TGGACTACAG TGGTCCTTCT 
CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG CTCTTCAACC CTTACTACGG ACTCTTTGAG 
TACTCTGCAA ATGATACTTA CACGGTGCAG ATCAGCCCCA TGTCTGCGTT TGTAGAAAAC 
CACCTGGAAT GGTTCAGGTT TAGTGGTCGT ATCCTAGGCC TGGCTTTGAT CCATCAGTAC 
CTTCTGGATG CTTTCTTCAC AAGGCCATTC TATAAGGCAC TCCTGAGGCT GCCCTGTGAT 
TTGAGTGATC TGGAATATTT GGACGAGGAA TTCCACCAGA GCTTGCAGTG GATGAAGGAC 
AACAACATCA CAGATATCCT CGACCTCACT TTCACTGTTA ATGAAGAGGT TTTTGGACAG 
GTAACAGAAA GGGAGTTAAA ATCTGGAGGA GCCAACACCC AGGTGACGGA GAAGAACAAG 
AAGGAGTACA TCGAGAGGAT GGTCAAGTGG CGGGTGGAGC GTGGCGTGGT GCAGCAGACT 
GAGGCACTGG TGCGGGGCTT CTACGAGGTT GTAGACTCGA GGCTCGTCTC AGTGTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATAGCTGGC ACCGCAGAGA TCGACCTGAA TGACTGGCGG 
AATAACACTG AGTATCGGGG AGGTTACCAT GATGGGCATC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AGCGCTTCAA TAATGAGCAG AGATTAAGAT TACTTCAGTT TGTCACGGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCT GCCCTTCGTG GAAGCAATGG GCTTCGGCGT 
TTCTGCATAG AGAAATGGGG GAAAATTACT TCTCTTCCCA GGGCACACAC GTGCTTCAAC 
CGATTGGATC TTCCACCATA TCCCTCATAC ACTATGTTGT ACGAAAAGCT TTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005319278.1
CDS	603..5435
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005319221.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005319221.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAGTAAGCCA GCTTCCTTCC 
TTGAGACCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAA 
GCTCGAATTG ACAGCCATGG GCGGGTCTTT TATGTGGACC ATGTGAACCG TACCACCACC 
TGGCAGCGTC CAACAGCAGC AGCCACCCCA GACGGGATGC GGAGATCCGG GTCCATCCAG 
CAGATGGAGC AGCTCAACAG GCGGTATCAA AATATTCAGC GGACCATTGC AACAGAAAGA 
TCTGAAGAGG ATTCTGGCAG CCAAAGCTGT GAGCAAGTCC CAGCCGGAGG AGGTGGAGGT 
GGAGGGAGTG ACTCAGAGGC TGAATCTCCC CAATCAAGCT TAGATCAGAG GAGAGAAGGG 
TCACTTTCTC CAGTTAACTC ACAAAAAATC ACCTTGCTGC TGCAGTCCCC AGCCGTCAAG 
TTCATCACCA ACCCAGAATT CTTCACTGTG CTGCATGCCA ATTATAGTGC CTACCGAGTC 
TTCACCAGTA GCACCTGCTT AAAGCACATG ATTCTGAAAG TCCGAAGAGA CGCTCGCAAT 
TTTGAACGCT ACCAGCACAA CCGGGACTTG GTGAATTTCA TCAACATGTT CGCAGACACT 
CGGCTGGAAC TGCCCCGAGG CTGGGAGATC AAAACAGACC AGCAGGGGAA GTCTTTTTTT 
GTGGACCACA ACAGTCGAGC TACCACTTTC ATTGACCCCC GAATCCCTCT TCAGAATGGT 
CGTCTTCCCA ATCATCTGAC TCATCGACAG CACCTCCAGA GGCTCCGAAG TTACAGCGCT 
GGAGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCCTCCT TACTGGCCAG GCCAGGACAC 
AGCCTAGTAG CTGCTATTCG AAGCCAACAC CAACATGAAG CATTGCCACT GGCATATAAT 
GACAAGATCG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAGCGTCAG 
CCAAGCTTGG CAAGAAATCA CACACTCAGG GAGAAAATCC ATTATATTCG GACCGAGGGT 
AATCATGGGC TTGAGAAGTT GTCCTGTGAT GCAGATCTAG TTATTTTGCT GAGCCTCTTT 
GAAGAAGAGA TTATGTCCTA TGTACCCCTG CAGGCTGCCT TCCACCCCGG GTATAGCTTT 
TCTCCCCGCT GTTCACCCTG TTCTTCACCT CAGAACTCTC CAGGTTTACA GAGAGCCAGT 
GCAAGAGCCC CTTCACCATA TCGGAGAGAC TTTGAGGCCA AGCTCCGCAA TTTCTATCGG 
AAACTAGAAG CCAAAGGATT TGGTCAGGGT CCAGGGAAAA TTAAGCTCAT TATTCGTCGG 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCCT ATTCCCGGAA GGAGCTCCAG 
AGAAACAAGC TCTACGTCAC CTTTGTTGGA GAGGAGGGCC TGGACTACAG TGGTCCTTCT 
CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG CTCTTCAACC CTTACTACGG ACTCTTTGAG 
TACTCTGCAA ATGATACTTA CACGGTGCAG ATCAGCCCCA TGTCTGCGTT TGTAGAAAAC 
CACCTGGAAT GGTTCAGGTT TAGTGGTCGT ATCCTAGGCC TGGCTTTGAT CCATCAGTAC 
CTTCTGGATG CTTTCTTCAC AAGGCCATTC TATAAGGCAC TCCTGAGGCT GCCCTGTGAT 
TTGAGTGATC TGGAATATTT GGACGAGGAA TTCCACCAGA GCTTGCAGTG GATGAAGGAC 
AACAACATCA CAGATATCCT CGACCTCACT TTCACTGTTA ATGAAGAGGT TTTTGGACAG 
GTAACAGAAA GGGAGTTAAA ATCTGGAGGA GCCAACACCC AGGTGACGGA GAAGAACAAG 
AAGGAGTACA TCGAGAGGAT GGTCAAGTGG CGGGTGGAGC GTGGCGTGGT GCAGCAGACT 
GAGGCACTGG TGCGGGGCTT CTACGAGGTT GTAGACTCGA GGCTCGTCTC AGTGTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATAGCTGGC ACCGCAGAGA TCGACCTGAA TGACTGGCGG 
AATAACACTG AGTATCGGGG AGGTTACCAT GATGGGCATC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AGCGCTTCAA TAATGAGCAG AGATTAAGAT TACTTCAGTT TGTCACGGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCT GCCCTTCGTG GAAGCAATGG GCTTCGGCGT 
TTCTGCATAG AGAAATGGGG GAAAATTACT TCTCTTCCCA GGGCACACAC GTGCTTCAAC 
CGATTGGATC TTCCACCATA TCCCTCATAC ACTATGTTGT ACGAAAAGCT TTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_005319222.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_005319279.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

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CloneID	OIc50426
Clone ID Related Accession (Same CDS sequence)	XM_005319222.3 , XM_005319222.2
Accession Version	XM_005319222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005319222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG ACTGGGAAGC TCGAATTGAC AGCCATGGGC GGGTCTTTTA TGTGGACCAT 
GTGAACCGTA CCACCACCTG GCAGCGTCCA ACAGCAGCAG CCACCCCAGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGCAG CTCAACAGGC GGTATCAAAA TATTCAGCGG 
ACCATTGCAA CAGAAAGATC TGAAGAGGAT TCTGGCAGCC AAAGCTGTGA GCAAGTCCCA 
GCCGGAGGAG GTGGAGGTGG AGGGAGTGAC TCAGAGGCTG AATCTCCCCA ATCAAGCTTA 
GATCAGAGGA GAGAAGGGTC ACTTTCTCCA GTTAACTCAC AAAAAATCAC CTTGCTGCTG 
CAGTCCCCAG CCGTCAAGTT CATCACCAAC CCAGAATTCT TCACTGTGCT GCATGCCAAT 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTAA AGCACATGAT TCTGAAAGTC 
CGAAGAGACG CTCGCAATTT TGAACGCTAC CAGCACAACC GGGACTTGGT GAATTTCATC 
AACATGTTCG CAGACACTCG GCTGGAACTG CCCCGAGGCT GGGAGATCAA AACAGACCAG 
CAGGGGAAGT CTTTTTTTGT GGACCACAAC AGTCGAGCTA CCACTTTCAT TGACCCCCGA 
ATCCCTCTTC AGAATGGTCG TCTTCCCAAT CATCTGACTC ATCGACAGCA CCTCCAGAGG 
CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCCTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGAAGCA 
TTGCCACTGG CATATAATGA CAAGATCGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AGCGTCAGCC AAGCTTGGCA AGAAATCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CCGAGGGTAA TCATGGGCTT GAGAAGTTGT CCTGTGATGC AGATCTAGTT 
ATTTTGCTGA GCCTCTTTGA AGAAGAGATT ATGTCCTATG TACCCCTGCA GGCTGCCTTC 
CACCCCGGGT ATAGCTTTTC TCCCCGCTGT TCACCCTGTT CTTCACCTCA GAACTCTCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCATATC GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTATCGGAA ACTAGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATTA TTCGTCGGGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGAG AAACAAGCTC TACGTCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCTCG AGAGTTCTTC TTCCTTTTGT CTCAGGAGCT CTTCAACCCT 
TACTACGGAC TCTTTGAGTA CTCTGCAAAT GATACTTACA CGGTGCAGAT CAGCCCCATG 
TCTGCGTTTG TAGAAAACCA CCTGGAATGG TTCAGGTTTA GTGGTCGTAT CCTAGGCCTG 
GCTTTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GGCCATTCTA TAAGGCACTC 
CTGAGGCTGC CCTGTGATTT GAGTGATCTG GAATATTTGG ACGAGGAATT CCACCAGAGC 
TTGCAGTGGA TGAAGGACAA CAACATCACA GATATCCTCG ACCTCACTTT CACTGTTAAT 
GAAGAGGTTT TTGGACAGGT AACAGAAAGG GAGTTAAAAT CTGGAGGAGC CAACACCCAG 
GTGACGGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TCAAGTGGCG GGTGGAGCGT 
GGCGTGGTGC AGCAGACTGA GGCACTGGTG CGGGGCTTCT ACGAGGTTGT AGACTCGAGG 
CTCGTCTCAG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TAGCTGGCAC CGCAGAGATC 
GACCTGAATG ACTGGCGGAA TAACACTGAG TATCGGGGAG GTTACCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAG CGCTTCAATA ATGAGCAGAG ATTAAGATTA 
CTTCAGTTTG TCACGGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCTGC CCTTCGTGGA 
AGCAATGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACTTC TCTTCCCAGG 
GCACACACGT GCTTCAACCG ATTGGATCTT CCACCATATC CCTCATACAC TATGTTGTAC 
GAAAAGCTTT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005319279.1
CDS	1034..5764
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005319222.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005319222.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG ACTGGGAAGC TCGAATTGAC AGCCATGGGC GGGTCTTTTA TGTGGACCAT 
GTGAACCGTA CCACCACCTG GCAGCGTCCA ACAGCAGCAG CCACCCCAGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGCAG CTCAACAGGC GGTATCAAAA TATTCAGCGG 
ACCATTGCAA CAGAAAGATC TGAAGAGGAT TCTGGCAGCC AAAGCTGTGA GCAAGTCCCA 
GCCGGAGGAG GTGGAGGTGG AGGGAGTGAC TCAGAGGCTG AATCTCCCCA ATCAAGCTTA 
GATCAGAGGA GAGAAGGGTC ACTTTCTCCA GTTAACTCAC AAAAAATCAC CTTGCTGCTG 
CAGTCCCCAG CCGTCAAGTT CATCACCAAC CCAGAATTCT TCACTGTGCT GCATGCCAAT 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTAA AGCACATGAT TCTGAAAGTC 
CGAAGAGACG CTCGCAATTT TGAACGCTAC CAGCACAACC GGGACTTGGT GAATTTCATC 
AACATGTTCG CAGACACTCG GCTGGAACTG CCCCGAGGCT GGGAGATCAA AACAGACCAG 
CAGGGGAAGT CTTTTTTTGT GGACCACAAC AGTCGAGCTA CCACTTTCAT TGACCCCCGA 
ATCCCTCTTC AGAATGGTCG TCTTCCCAAT CATCTGACTC ATCGACAGCA CCTCCAGAGG 
CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCCTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGAAGCA 
TTGCCACTGG CATATAATGA CAAGATCGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AGCGTCAGCC AAGCTTGGCA AGAAATCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CCGAGGGTAA TCATGGGCTT GAGAAGTTGT CCTGTGATGC AGATCTAGTT 
ATTTTGCTGA GCCTCTTTGA AGAAGAGATT ATGTCCTATG TACCCCTGCA GGCTGCCTTC 
CACCCCGGGT ATAGCTTTTC TCCCCGCTGT TCACCCTGTT CTTCACCTCA GAACTCTCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCATATC GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTATCGGAA ACTAGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATTA TTCGTCGGGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGAG AAACAAGCTC TACGTCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCTCG AGAGTTCTTC TTCCTTTTGT CTCAGGAGCT CTTCAACCCT 
TACTACGGAC TCTTTGAGTA CTCTGCAAAT GATACTTACA CGGTGCAGAT CAGCCCCATG 
TCTGCGTTTG TAGAAAACCA CCTGGAATGG TTCAGGTTTA GTGGTCGTAT CCTAGGCCTG 
GCTTTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GGCCATTCTA TAAGGCACTC 
CTGAGGCTGC CCTGTGATTT GAGTGATCTG GAATATTTGG ACGAGGAATT CCACCAGAGC 
TTGCAGTGGA TGAAGGACAA CAACATCACA GATATCCTCG ACCTCACTTT CACTGTTAAT 
GAAGAGGTTT TTGGACAGGT AACAGAAAGG GAGTTAAAAT CTGGAGGAGC CAACACCCAG 
GTGACGGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TCAAGTGGCG GGTGGAGCGT 
GGCGTGGTGC AGCAGACTGA GGCACTGGTG CGGGGCTTCT ACGAGGTTGT AGACTCGAGG 
CTCGTCTCAG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TAGCTGGCAC CGCAGAGATC 
GACCTGAATG ACTGGCGGAA TAACACTGAG TATCGGGGAG GTTACCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAG CGCTTCAATA ATGAGCAGAG ATTAAGATTA 
CTTCAGTTTG TCACGGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCTGC CCTTCGTGGA 
AGCAATGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACTTC TCTTCCCAGG 
GCACACACGT GCTTCAACCG ATTGGATCTT CCACCATATC CCTCATACAC TATGTTGTAC 
GAAAAGCTTT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_005319222.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_005319279.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

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CloneID	OIc50426
Clone ID Related Accession (Same CDS sequence)	XM_005319222.3 , XM_005319222.2
Accession Version	XM_005319222.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005319222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG ACTGGGAAGC TCGAATTGAC AGCCATGGGC GGGTCTTTTA TGTGGACCAT 
GTGAACCGTA CCACCACCTG GCAGCGTCCA ACAGCAGCAG CCACCCCAGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGCAG CTCAACAGGC GGTATCAAAA TATTCAGCGG 
ACCATTGCAA CAGAAAGATC TGAAGAGGAT TCTGGCAGCC AAAGCTGTGA GCAAGTCCCA 
GCCGGAGGAG GTGGAGGTGG AGGGAGTGAC TCAGAGGCTG AATCTCCCCA ATCAAGCTTA 
GATCAGAGGA GAGAAGGGTC ACTTTCTCCA GTTAACTCAC AAAAAATCAC CTTGCTGCTG 
CAGTCCCCAG CCGTCAAGTT CATCACCAAC CCAGAATTCT TCACTGTGCT GCATGCCAAT 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTAA AGCACATGAT TCTGAAAGTC 
CGAAGAGACG CTCGCAATTT TGAACGCTAC CAGCACAACC GGGACTTGGT GAATTTCATC 
AACATGTTCG CAGACACTCG GCTGGAACTG CCCCGAGGCT GGGAGATCAA AACAGACCAG 
CAGGGGAAGT CTTTTTTTGT GGACCACAAC AGTCGAGCTA CCACTTTCAT TGACCCCCGA 
ATCCCTCTTC AGAATGGTCG TCTTCCCAAT CATCTGACTC ATCGACAGCA CCTCCAGAGG 
CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCCTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGAAGCA 
TTGCCACTGG CATATAATGA CAAGATCGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AGCGTCAGCC AAGCTTGGCA AGAAATCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CCGAGGGTAA TCATGGGCTT GAGAAGTTGT CCTGTGATGC AGATCTAGTT 
ATTTTGCTGA GCCTCTTTGA AGAAGAGATT ATGTCCTATG TACCCCTGCA GGCTGCCTTC 
CACCCCGGGT ATAGCTTTTC TCCCCGCTGT TCACCCTGTT CTTCACCTCA GAACTCTCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCATATC GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTATCGGAA ACTAGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATTA TTCGTCGGGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGAG AAACAAGCTC TACGTCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCTCG AGAGTTCTTC TTCCTTTTGT CTCAGGAGCT CTTCAACCCT 
TACTACGGAC TCTTTGAGTA CTCTGCAAAT GATACTTACA CGGTGCAGAT CAGCCCCATG 
TCTGCGTTTG TAGAAAACCA CCTGGAATGG TTCAGGTTTA GTGGTCGTAT CCTAGGCCTG 
GCTTTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GGCCATTCTA TAAGGCACTC 
CTGAGGCTGC CCTGTGATTT GAGTGATCTG GAATATTTGG ACGAGGAATT CCACCAGAGC 
TTGCAGTGGA TGAAGGACAA CAACATCACA GATATCCTCG ACCTCACTTT CACTGTTAAT 
GAAGAGGTTT TTGGACAGGT AACAGAAAGG GAGTTAAAAT CTGGAGGAGC CAACACCCAG 
GTGACGGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TCAAGTGGCG GGTGGAGCGT 
GGCGTGGTGC AGCAGACTGA GGCACTGGTG CGGGGCTTCT ACGAGGTTGT AGACTCGAGG 
CTCGTCTCAG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TAGCTGGCAC CGCAGAGATC 
GACCTGAATG ACTGGCGGAA TAACACTGAG TATCGGGGAG GTTACCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAG CGCTTCAATA ATGAGCAGAG ATTAAGATTA 
CTTCAGTTTG TCACGGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCTGC CCTTCGTGGA 
AGCAATGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACTTC TCTTCCCAGG 
GCACACACGT GCTTCAACCG ATTGGATCTT CCACCATATC CCTCATACAC TATGTTGTAC 
GAAAAGCTTT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005319279.1
CDS	368..5098
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005319222.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005319222.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCACG AAACTCACAG AGCCGACGCC GGTGCAAGGA GCCACTCCGC 
TACAGCTACA ACCCTGACCA GTTCCACAAC ATGGATATCA GGAATGGACC TCATGATGGT 
GTGACCATCC CCCGCTCCAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGTTCCACA 
CTCATGGTTA GCAGCTCCTA CTACTCCATA GGGCACTCAC AGGATCTGGT GATCCACTGG 
GACATTAAGG AGGAAGTGGA CGCTGGAGAC TGGATTGGCA TGTACCTCAT AGATGAAGTC 
TTGTCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACCAA GATCTGTTTC 
AAATACTACC ATGGAGTGAG TGGAGCCCTG AGAGCTACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CGATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTCCCTGCCC TTCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACAGTG 
AACCCCATCT GGCAGGCGGA ACAATTCAGT TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AAGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCGATGC CTGTCCAGAG ACTCCTGGAG AGACACGCCA TAGGGGATAG AGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC TGAACCTGAG 
TCAGCAGAAA TTCAGGACAG CCTCATGAAC AACCTGGTTG AAAGCAACTG TGCGGAGCCT 
CCAAATGATG CTCCAGAGCC CTGTGAGAGC TTGAGGCCAG AGCCAACCAA CGAGGGCACT 
GCAGTGGACA GTTCAGGACA CCAGAGCCTG GAGCATGCCA GGCCAGTTGA GGAAGGGGCA 
GGTGCCACTG AGGCAGGAGA TGATGGCAGG ATCTCTGAGG GACCTGAAGG GGTTGGGGAG 
CTTCTGGCCC CAGGACAACA GGACCCCGAG TCTGCCCTGA GTGCAGAAGA ACTAGCCGAG 
AGGCTCGACC TGGATGAACA GGTACCACCA TCGCTACTTC TGGAAGATGG TGAGGCTTCC 
GCCAACAAGA ATGAGCCGGT GGAGGAGGAG GCAGCACCCG AGAGCCGGGC CAGGGGGGAG 
GAGGAAGAGA AAGGCCAGGG TGAAGAGGAT GAGGAGGGGG ATGTGCCTAC CCTGGAGCAC 
GGAGAGGGCA GACTGCAGCT GCGAGCCTCA GTGAAGAGAA AAAGCAGACC ATGCTCTTTG 
CCTGTGTCCG AGCTGGAGAC CGTGATCGCG TCGGCCTGCG GGGACCCCGA GACCCCGCGG 
ACACACTACA TTCGCATCCA TAACCTGCTC CACAGCATGC CGACGGCCCA CAGCGGGAGC 
GCAGCAGAGG AAGAGGACGG GGCGGAAGAG GAGTCAACCC TCAAGGATTC CTCCGAGAAG 
GATGGGTTCA GCGAGGTGGA CACGGTGGCG GCGGATCCGC CGGCCCTGGA AGAGGATGGA 
GGAGAGTTCG AGGGTGCCAC TCCGGGTATG GCACCCCCTG GCCACCTTGG GGGCCACTTC 
CGTAGTTTGA CAAATGGCGC GGGTCCTGAA GGTGACACGC TTCCCAGCAC TGGGAGTGAG 
AGCGACTCCA GCCCCCGGCA AGGCGGGGAC CACAGCTGCG AGGGCTGTGA CGCGTCGTGC 
TGCAGCCCCT CCTGCTACAG CTCCTCGTGC TACAGCACGT CCTGCTACAG CAGCTCCTGC 
TACAGCGCCT CCTGCTACAG CCCCTCCTGC TACAACGGCA ACAACCGGTT CGCCAGCCAC 
ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG ATCTCCGAGA GCACCGTCTT CTCGTCGCAA 
GACGACGAGG AAGAGGAGAA CAGCGCGTTC GAGTCGGTGC CTGACTCGGT ACAGAGCCCG 
GAGCTGGACC CGGAGTCGAC CAATGGTGCT GGGCCATGGC AGGACGAGCT GGCTGCCCCT 
GGTGGGAACC TGACAAGAAC CGCTGAAGGT CTGGAGTCCC CCATGGCAGG TCCAAGCAAT 
CGGAGAGAAG ACTGGGAAGC TCGAATTGAC AGCCATGGGC GGGTCTTTTA TGTGGACCAT 
GTGAACCGTA CCACCACCTG GCAGCGTCCA ACAGCAGCAG CCACCCCAGA CGGGATGCGG 
AGATCCGGGT CCATCCAGCA GATGGAGCAG CTCAACAGGC GGTATCAAAA TATTCAGCGG 
ACCATTGCAA CAGAAAGATC TGAAGAGGAT TCTGGCAGCC AAAGCTGTGA GCAAGTCCCA 
GCCGGAGGAG GTGGAGGTGG AGGGAGTGAC TCAGAGGCTG AATCTCCCCA ATCAAGCTTA 
GATCAGAGGA GAGAAGGGTC ACTTTCTCCA GTTAACTCAC AAAAAATCAC CTTGCTGCTG 
CAGTCCCCAG CCGTCAAGTT CATCACCAAC CCAGAATTCT TCACTGTGCT GCATGCCAAT 
TATAGTGCCT ACCGAGTCTT CACCAGTAGC ACCTGCTTAA AGCACATGAT TCTGAAAGTC 
CGAAGAGACG CTCGCAATTT TGAACGCTAC CAGCACAACC GGGACTTGGT GAATTTCATC 
AACATGTTCG CAGACACTCG GCTGGAACTG CCCCGAGGCT GGGAGATCAA AACAGACCAG 
CAGGGGAAGT CTTTTTTTGT GGACCACAAC AGTCGAGCTA CCACTTTCAT TGACCCCCGA 
ATCCCTCTTC AGAATGGTCG TCTTCCCAAT CATCTGACTC ATCGACAGCA CCTCCAGAGG 
CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCCTCCTTA 
CTGGCCAGGC CAGGACACAG CCTAGTAGCT GCTATTCGAA GCCAACACCA ACATGAAGCA 
TTGCCACTGG CATATAATGA CAAGATCGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AGCGTCAGCC AAGCTTGGCA AGAAATCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CCGAGGGTAA TCATGGGCTT GAGAAGTTGT CCTGTGATGC AGATCTAGTT 
ATTTTGCTGA GCCTCTTTGA AGAAGAGATT ATGTCCTATG TACCCCTGCA GGCTGCCTTC 
CACCCCGGGT ATAGCTTTTC TCCCCGCTGT TCACCCTGTT CTTCACCTCA GAACTCTCCA 
GGTTTACAGA GAGCCAGTGC AAGAGCCCCT TCACCATATC GGAGAGACTT TGAGGCCAAG 
CTCCGCAATT TCTATCGGAA ACTAGAAGCC AAAGGATTTG GTCAGGGTCC AGGGAAAATT 
AAGCTCATTA TTCGTCGGGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCCTAT 
TCCCGGAAGG AGCTCCAGAG AAACAAGCTC TACGTCACCT TTGTTGGAGA GGAGGGCCTG 
GACTACAGTG GTCCTTCTCG AGAGTTCTTC TTCCTTTTGT CTCAGGAGCT CTTCAACCCT 
TACTACGGAC TCTTTGAGTA CTCTGCAAAT GATACTTACA CGGTGCAGAT CAGCCCCATG 
TCTGCGTTTG TAGAAAACCA CCTGGAATGG TTCAGGTTTA GTGGTCGTAT CCTAGGCCTG 
GCTTTGATCC ATCAGTACCT TCTGGATGCT TTCTTCACAA GGCCATTCTA TAAGGCACTC 
CTGAGGCTGC CCTGTGATTT GAGTGATCTG GAATATTTGG ACGAGGAATT CCACCAGAGC 
TTGCAGTGGA TGAAGGACAA CAACATCACA GATATCCTCG ACCTCACTTT CACTGTTAAT 
GAAGAGGTTT TTGGACAGGT AACAGAAAGG GAGTTAAAAT CTGGAGGAGC CAACACCCAG 
GTGACGGAGA AGAACAAGAA GGAGTACATC GAGAGGATGG TCAAGTGGCG GGTGGAGCGT 
GGCGTGGTGC AGCAGACTGA GGCACTGGTG CGGGGCTTCT ACGAGGTTGT AGACTCGAGG 
CTCGTCTCAG TGTTTGATGC CAGGGAGCTG GAGCTGGTGA TAGCTGGCAC CGCAGAGATC 
GACCTGAATG ACTGGCGGAA TAACACTGAG TATCGGGGAG GTTACCATGA TGGGCATCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAG CGCTTCAATA ATGAGCAGAG ATTAAGATTA 
CTTCAGTTTG TCACGGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCTGC CCTTCGTGGA 
AGCAATGGGC TTCGGCGTTT CTGCATAGAG AAATGGGGGA AAATTACTTC TCTTCCCAGG 
GCACACACGT GCTTCAACCG ATTGGATCTT CCACCATATC CCTCATACAC TATGTTGTAC 
GAAAAGCTTT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_021722298.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_021577973.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

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CloneID	OIc75360
Clone ID Related Accession (Same CDS sequence)	XM_021722298.1
Accession Version	XM_021722298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTCCGG GACTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC ACGAAACTCA CAGAGCCGAC GCCGGTGCAA GGAGCCACTC 
CGCTACAGCT ACAACCCTGA CCAGTTCCAC AACATGGATA TCAGGAATGG ACCTCATGAT 
GGTGTGACCA TCCCCCGCTC CACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGTTCC 
ACACTCATGG TTAGCAGCTC CTACTACTCC ATAGGGCACT CACAGGATCT GGTGATCCAC 
TGGGACATTA AGGAGGAAGT GGACGCTGGA GACTGGATTG GCATGTACCT CATAGATGAA 
GTCTTGTCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC CAAGATCTGT 
TTCAAATACT ACCATGGAGT GAGTGGAGCC CTGAGAGCTA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT CCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCGATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCTG CCCTTCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACA 
GTGAACCCCA TCTGGCAGGC GGAACAATTC AGTTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAAGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCGA TGCCTGTCCA GAGACTCCTG GAGAGACACG CCATAGGGGA TAGAGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACTGAACCT 
GAGTCAGCAG AAATTCAGGA CAGCCTCATG AACAACCTGG TTGAAAGCAA CTGTGCGGAG 
CCTCCAAATG ATGCTCCAGA GCCCTGTGAG AGCTTGAGGC CAGAGCCAAC CAACGAGGGC 
ACTGCAGTGG ACAGTTCAGG ACACCAGAGC CTGGAGCATG CCAGGCCAGT TGAGGAAGGG 
GCAGGTGCCA CTGAGGCAGG AGATGATGGC AGGATCTCTG AGGGACCTGA AGGGGTTGGG 
GAGCTTCTGG CCCCAGGACA ACAGGACCCC GAGTCTGCCC TGAGTGCAGA AGAACTAGCC 
GAGAGGCTCG ACCTGGATGA ACAGGTACCA CCATCGCTAC TTCTGGAAGA TGGTGAGGCT 
TCCGCCAACA AGAATGAGCC GGTGGAGGAG GAGGCAGCAC CCGAGAGCCG GGCCAGGGGG 
GAGGAGGAAG AGAAAGGCCA GGGTGAAGAG GATGAGGAGG GGGATGTGCC TACCCTGGAG 
CACGGAGAGG GCAGACTGCA GCTGCGAGCC TCAGTGAAGA GAAAAAGCAG ACCATGCTCT 
TTGCCTGTGT CCGAGCTGGA GACCGTGATC GCGTCGGCCT GCGGGGACCC CGAGACCCCG 
CGGACACACT ACATTCGCAT CCATAACCTG CTCCACAGCA TGCCGACGGC CCACAGCGGG 
AGCGCAGCAG AGGAAGAGGA CGGGGCGGAA GAGGAGTCAA CCCTCAAGGA TTCCTCCGAG 
AAGGATGGGT TCAGCGAGGT GGACACGGTG GCGGCGGATC CGCCGGCCCT GGAAGAGGAT 
GGAGGAGAGT TCGAGGGTGC CACTCCGGGT ATGGCACCCC CTGGCCACCT TGGGGGCCAC 
TTCCGTAGTT TGACAAATGG CGCGGGTCCT GAAGGTGACA CGCTTCCCAG CACTGGGAGT 
GAGAGCGACT CCAGCCCCCG GCAAGGCGGG GACCACAGCT GCGAGGGCTG TGACGCGTCG 
TGCTGCAGCC CCTCCTGCTA CAGCTCCTCG TGCTACAGCA CGTCCTGCTA CAGCAGCTCC 
TGCTACAGCG CCTCCTGCTA CAGCCCCTCC TGCTACAACG GCAACAACCG GTTCGCCAGC 
CACACGCGCT TCTCCTCCGT GGACAGCGCC AAGATCTCCG AGAGCACCGT CTTCTCGTCG 
CAAGACGACG AGGAAGAGGA GAACAGCGCG TTCGAGTCGG TGCCTGACTC GGTACAGAGC 
CCGGAGCTGG ACCCGGAGTC GACCAATGGT GCTGGGCCAT GGCAGGACGA GCTGGCTGCC 
CCTGGTGGGA ACCTGACAAG AACCGCTGAA GGTCTGGAGT CCCCCATGGC AGGTCCAAGC 
AATCGGAGAG AAGGTGAATG TCCTATACTC CATAATTCCC AGCCAGTAAG CCAGCTTCCT 
TCCTTGAGAC CTGAACATCA TCACTACCCA ACAATCGATG AGCCTCTTCC ACCAAACTGG 
GAAGCTCGAA TTGACAGCCA TGGGCGGGTC TTTTATGTGG ACCATGTGAA CCGTACCACC 
ACCTGGCAGC GTCCAACAGC AGCAGCCACC CCAGACGGGA TGCGGAGATC CGGGTCCATC 
CAGCAGATGG AGCAGCTCAA CAGGCGGTAT CAAAATATTC AGCGGACCAT TGCAACAGAA 
AGATCTGAAG AGGATTCTGG CAGCCAAAGC TGTGAGCAAG TCCCAGCCGG AGGAGGTGGA 
GGTGGAGGGA GTGACTCAGA GGCTGAATCT CCCCAATCAA GCTTAGATCA GAGGAGAGAA 
GGGTCACTTT CTCCAGTTAA CTCACAAAAA ATCACCTTGC TGCTGCAGTC CCCAGCCGTC 
AAGTTCATCA CCAACCCAGA ATTCTTCACT GTGCTGCATG CCAATTATAG TGCCTACCGA 
GTCTTCACCA GTAGCACCTG CTTAAAGCAC ATGATTCTGA AAGTCCGAAG AGACGCTCGC 
AATTTTGAAC GCTACCAGCA CAACCGGGAC TTGGTGAATT TCATCAACAT GTTCGCAGAC 
ACTCGGCTGG AACTGCCCCG AGGCTGGGAG ATCAAAACAG ACCAGCAGGG GAAGTCTTTT 
TTTGTGGACC ACAACAGTCG AGCTACCACT TTCATTGACC CCCGAATCCC TCTTCAGAAT 
GGTCGTCTTC CCAATCATCT GACTCATCGA CAGCACCTCC AGAGGCTCCG AAGTTACAGC 
GCTGGAGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCCT CCTTACTGGC CAGGCCAGGA 
CACAGCCTAG TAGCTGCTAT TCGAAGCCAA CACCAACATG AAGCATTGCC ACTGGCATAT 
AATGACAAGA TCGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAGCGT 
CAGCCAAGCT TGGCAAGAAA TCACACACTC AGGGAGAAAA TCCATTATAT TCGGACCGAG 
GGTAATCATG GGCTTGAGAA GTTGTCCTGT GATGCAGATC TAGTTATTTT GCTGAGCCTC 
TTTGAAGAAG AGATTATGTC CTATGTACCC CTGCAGGCTG CCTTCCACCC CGGGTATAGC 
TTTTCTCCCC GCTGTTCACC CTGTTCTTCA CCTCAGAACT CTCCAGGTTT ACAGAGAGCC 
AGTGCAAGAG CCCCTTCACC ATATCGGAGA GACTTTGAGG CCAAGCTCCG CAATTTCTAT 
CGGAAACTAG AAGCCAAAGG ATTTGGTCAG GGTCCAGGGA AAATTAAGCT CATTATTCGT 
CGGGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CCTATTCCCG GAAGGAGCTC 
CAGAGAAACA AGCTCTACGT CACCTTTGTT GGAGAGGAGG GCCTGGACTA CAGTGGTCCT 
TCTCGAGAGT TCTTCTTCCT TTTGTCTCAG GAGCTCTTCA ACCCTTACTA CGGACTCTTT 
GAGTACTCTG CAAATGATAC TTACACGGTG CAGATCAGCC CCATGTCTGC GTTTGTAGAA 
AACCACCTGG AATGGTTCAG GTTTAGTGGT CGTATCCTAG GCCTGGCTTT GATCCATCAG 
TACCTTCTGG ATGCTTTCTT CACAAGGCCA TTCTATAAGG CACTCCTGAG GCTGCCCTGT 
GATTTGAGTG ATCTGGAATA TTTGGACGAG GAATTCCACC AGAGCTTGCA GTGGATGAAG 
GACAACAACA TCACAGATAT CCTCGACCTC ACTTTCACTG TTAATGAAGA GGTTTTTGGA 
CAGGTAACAG AAAGGGAGTT AAAATCTGGA GGAGCCAACA CCCAGGTGAC GGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTCAAG TGGCGGGTGG AGCGTGGCGT GGTGCAGCAG 
ACTGAGGCAC TGGTGCGGGG CTTCTACGAG GTTGTAGACT CGAGGCTCGT CTCAGTGTTT 
GATGCCAGGG AGCTGGAGCT GGTGATAGCT GGCACCGCAG AGATCGACCT GAATGACTGG 
CGGAATAACA CTGAGTATCG GGGAGGTTAC CATGATGGGC ATCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAGCGCTT CAATAATGAG CAGAGATTAA GATTACTTCA GTTTGTCACG 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCTGCCCTTC GTGGAAGCAA TGGGCTTCGG 
CGTTTCTGCA TAGAGAAATG GGGGAAAATT ACTTCTCTTC CCAGGGCACA CACGTGCTTC 
AACCGATTGG ATCTTCCACC ATATCCCTCA TACACTATGT TGTACGAAAA GCTTTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021577973.1
CDS	16..4851
Translation	MICAQSGTAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021722298.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722298.1

ORF Insert Sequence:

ATGATCTGTG CCCAGTCCGG GACTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC ACGAAACTCA CAGAGCCGAC GCCGGTGCAA GGAGCCACTC 
CGCTACAGCT ACAACCCTGA CCAGTTCCAC AACATGGATA TCAGGAATGG ACCTCATGAT 
GGTGTGACCA TCCCCCGCTC CACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGTTCC 
ACACTCATGG TTAGCAGCTC CTACTACTCC ATAGGGCACT CACAGGATCT GGTGATCCAC 
TGGGACATTA AGGAGGAAGT GGACGCTGGA GACTGGATTG GCATGTACCT CATAGATGAA 
GTCTTGTCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC CAAGATCTGT 
TTCAAATACT ACCATGGAGT GAGTGGAGCC CTGAGAGCTA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT CCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCGATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCTG CCCTTCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACA 
GTGAACCCCA TCTGGCAGGC GGAACAATTC AGTTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAAGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCGA TGCCTGTCCA GAGACTCCTG GAGAGACACG CCATAGGGGA TAGAGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACTGAACCT 
GAGTCAGCAG AAATTCAGGA CAGCCTCATG AACAACCTGG TTGAAAGCAA CTGTGCGGAG 
CCTCCAAATG ATGCTCCAGA GCCCTGTGAG AGCTTGAGGC CAGAGCCAAC CAACGAGGGC 
ACTGCAGTGG ACAGTTCAGG ACACCAGAGC CTGGAGCATG CCAGGCCAGT TGAGGAAGGG 
GCAGGTGCCA CTGAGGCAGG AGATGATGGC AGGATCTCTG AGGGACCTGA AGGGGTTGGG 
GAGCTTCTGG CCCCAGGACA ACAGGACCCC GAGTCTGCCC TGAGTGCAGA AGAACTAGCC 
GAGAGGCTCG ACCTGGATGA ACAGGTACCA CCATCGCTAC TTCTGGAAGA TGGTGAGGCT 
TCCGCCAACA AGAATGAGCC GGTGGAGGAG GAGGCAGCAC CCGAGAGCCG GGCCAGGGGG 
GAGGAGGAAG AGAAAGGCCA GGGTGAAGAG GATGAGGAGG GGGATGTGCC TACCCTGGAG 
CACGGAGAGG GCAGACTGCA GCTGCGAGCC TCAGTGAAGA GAAAAAGCAG ACCATGCTCT 
TTGCCTGTGT CCGAGCTGGA GACCGTGATC GCGTCGGCCT GCGGGGACCC CGAGACCCCG 
CGGACACACT ACATTCGCAT CCATAACCTG CTCCACAGCA TGCCGACGGC CCACAGCGGG 
AGCGCAGCAG AGGAAGAGGA CGGGGCGGAA GAGGAGTCAA CCCTCAAGGA TTCCTCCGAG 
AAGGATGGGT TCAGCGAGGT GGACACGGTG GCGGCGGATC CGCCGGCCCT GGAAGAGGAT 
GGAGGAGAGT TCGAGGGTGC CACTCCGGGT ATGGCACCCC CTGGCCACCT TGGGGGCCAC 
TTCCGTAGTT TGACAAATGG CGCGGGTCCT GAAGGTGACA CGCTTCCCAG CACTGGGAGT 
GAGAGCGACT CCAGCCCCCG GCAAGGCGGG GACCACAGCT GCGAGGGCTG TGACGCGTCG 
TGCTGCAGCC CCTCCTGCTA CAGCTCCTCG TGCTACAGCA CGTCCTGCTA CAGCAGCTCC 
TGCTACAGCG CCTCCTGCTA CAGCCCCTCC TGCTACAACG GCAACAACCG GTTCGCCAGC 
CACACGCGCT TCTCCTCCGT GGACAGCGCC AAGATCTCCG AGAGCACCGT CTTCTCGTCG 
CAAGACGACG AGGAAGAGGA GAACAGCGCG TTCGAGTCGG TGCCTGACTC GGTACAGAGC 
CCGGAGCTGG ACCCGGAGTC GACCAATGGT GCTGGGCCAT GGCAGGACGA GCTGGCTGCC 
CCTGGTGGGA ACCTGACAAG AACCGCTGAA GGTCTGGAGT CCCCCATGGC AGGTCCAAGC 
AATCGGAGAG AAGGTGAATG TCCTATACTC CATAATTCCC AGCCAGTAAG CCAGCTTCCT 
TCCTTGAGAC CTGAACATCA TCACTACCCA ACAATCGATG AGCCTCTTCC ACCAAACTGG 
GAAGCTCGAA TTGACAGCCA TGGGCGGGTC TTTTATGTGG ACCATGTGAA CCGTACCACC 
ACCTGGCAGC GTCCAACAGC AGCAGCCACC CCAGACGGGA TGCGGAGATC CGGGTCCATC 
CAGCAGATGG AGCAGCTCAA CAGGCGGTAT CAAAATATTC AGCGGACCAT TGCAACAGAA 
AGATCTGAAG AGGATTCTGG CAGCCAAAGC TGTGAGCAAG TCCCAGCCGG AGGAGGTGGA 
GGTGGAGGGA GTGACTCAGA GGCTGAATCT CCCCAATCAA GCTTAGATCA GAGGAGAGAA 
GGGTCACTTT CTCCAGTTAA CTCACAAAAA ATCACCTTGC TGCTGCAGTC CCCAGCCGTC 
AAGTTCATCA CCAACCCAGA ATTCTTCACT GTGCTGCATG CCAATTATAG TGCCTACCGA 
GTCTTCACCA GTAGCACCTG CTTAAAGCAC ATGATTCTGA AAGTCCGAAG AGACGCTCGC 
AATTTTGAAC GCTACCAGCA CAACCGGGAC TTGGTGAATT TCATCAACAT GTTCGCAGAC 
ACTCGGCTGG AACTGCCCCG AGGCTGGGAG ATCAAAACAG ACCAGCAGGG GAAGTCTTTT 
TTTGTGGACC ACAACAGTCG AGCTACCACT TTCATTGACC CCCGAATCCC TCTTCAGAAT 
GGTCGTCTTC CCAATCATCT GACTCATCGA CAGCACCTCC AGAGGCTCCG AAGTTACAGC 
GCTGGAGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCCT CCTTACTGGC CAGGCCAGGA 
CACAGCCTAG TAGCTGCTAT TCGAAGCCAA CACCAACATG AAGCATTGCC ACTGGCATAT 
AATGACAAGA TCGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAGCGT 
CAGCCAAGCT TGGCAAGAAA TCACACACTC AGGGAGAAAA TCCATTATAT TCGGACCGAG 
GGTAATCATG GGCTTGAGAA GTTGTCCTGT GATGCAGATC TAGTTATTTT GCTGAGCCTC 
TTTGAAGAAG AGATTATGTC CTATGTACCC CTGCAGGCTG CCTTCCACCC CGGGTATAGC 
TTTTCTCCCC GCTGTTCACC CTGTTCTTCA CCTCAGAACT CTCCAGGTTT ACAGAGAGCC 
AGTGCAAGAG CCCCTTCACC ATATCGGAGA GACTTTGAGG CCAAGCTCCG CAATTTCTAT 
CGGAAACTAG AAGCCAAAGG ATTTGGTCAG GGTCCAGGGA AAATTAAGCT CATTATTCGT 
CGGGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CCTATTCCCG GAAGGAGCTC 
CAGAGAAACA AGCTCTACGT CACCTTTGTT GGAGAGGAGG GCCTGGACTA CAGTGGTCCT 
TCTCGAGAGT TCTTCTTCCT TTTGTCTCAG GAGCTCTTCA ACCCTTACTA CGGACTCTTT 
GAGTACTCTG CAAATGATAC TTACACGGTG CAGATCAGCC CCATGTCTGC GTTTGTAGAA 
AACCACCTGG AATGGTTCAG GTTTAGTGGT CGTATCCTAG GCCTGGCTTT GATCCATCAG 
TACCTTCTGG ATGCTTTCTT CACAAGGCCA TTCTATAAGG CACTCCTGAG GCTGCCCTGT 
GATTTGAGTG ATCTGGAATA TTTGGACGAG GAATTCCACC AGAGCTTGCA GTGGATGAAG 
GACAACAACA TCACAGATAT CCTCGACCTC ACTTTCACTG TTAATGAAGA GGTTTTTGGA 
CAGGTAACAG AAAGGGAGTT AAAATCTGGA GGAGCCAACA CCCAGGTGAC GGAGAAGAAC 
AAGAAGGAGT ACATCGAGAG GATGGTCAAG TGGCGGGTGG AGCGTGGCGT GGTGCAGCAG 
ACTGAGGCAC TGGTGCGGGG CTTCTACGAG GTTGTAGACT CGAGGCTCGT CTCAGTGTTT 
GATGCCAGGG AGCTGGAGCT GGTGATAGCT GGCACCGCAG AGATCGACCT GAATGACTGG 
CGGAATAACA CTGAGTATCG GGGAGGTTAC CATGATGGGC ATCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAGCGCTT CAATAATGAG CAGAGATTAA GATTACTTCA GTTTGTCACG 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCTGCCCTTC GTGGAAGCAA TGGGCTTCGG 
CGTTTCTGCA TAGAGAAATG GGGGAAAATT ACTTCTCTTC CCAGGGCACA CACGTGCTTC 
AACCGATTGG ATCTTCCACC ATATCCCTCA TACACTATGT TGTACGAAAA GCTTTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_021722300.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel) -> XP_021577975.1 Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

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CloneID	OIc75361
Clone ID Related Accession (Same CDS sequence)	XM_021722300.1
Accession Version	XM_021722300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936478.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTCCGG GACTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC ACGAAACTCA CAGAGCCGAC GCCGGTGCAA GGAGCCACTC 
CGCTACAGCT ACAACCCTGA CCAGTTCCAC AACATGGATA TCAGGAATGG ACCTCATGAT 
GGTGTGACCA TCCCCCGCTC CACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGTTCC 
ACACTCATGG TTAGCAGCTC CTACTACTCC ATAGGGCACT CACAGGATCT GGTGATCCAC 
TGGGACATTA AGGAGGAAGT GGACGCTGGA GACTGGATTG GCATGTACCT CATAGATGAA 
GTCTTGTCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC CAAGATCTGT 
TTCAAATACT ACCATGGAGT GAGTGGAGCC CTGAGAGCTA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT CCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCGATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCTG CCCTTCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACA 
GTGAACCCCA TCTGGCAGGC GGAACAATTC AGTTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAAGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCGA TGCCTGTCCA GAGACTCCTG GAGAGACACG CCATAGGGGA TAGAGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACTGAACCT 
GAGTCAGCAG AAATTCAGGA CAGCCTCATG AACAACCTGG TTGAAAGCAA CTGTGCGGAG 
CCTCCAAATG ATGCTCCAGA GCCCTGTGAG AGCTTGAGGC CAGAGCCAAC CAACGAGGGC 
ACTGCAGTGG ACAGTTCAGG ACACCAGAGC CTGGAGCATG CCAGGCCAGT TGAGGAAGGG 
GCAGGTGCCA CTGAGGCAGG AGATGATGGC AGGATCTCTG AGGGACCTGA AGGGGTTGGG 
GAGCTTCTGG CCCCAGGACA ACAGGACCCC GAGTCTGCCC TGAGTGCAGA AGAACTAGCC 
GAGAGGCTCG ACCTGGATGA ACAGGTACCA CCATCGCTAC TTCTGGAAGA TGGTGAGGCT 
TCCGCCAACA AGAATGAGCC GGTGGAGGAG GAGGCAGCAC CCGAGAGCCG GGCCAGGGGG 
GAGGAGGAAG AGAAAGGCCA GGGTGAAGAG GATGAGGAGG GGGATGTGCC TACCCTGGAG 
CACGGAGAGG GCAGACTGCA GCTGCGAGCC TCAGTGAAGA GAAAAAGCAG ACCATGCTCT 
TTGCCTGTGT CCGAGCTGGA GACCGTGATC GCGTCGGCCT GCGGGGACCC CGAGACCCCG 
CGGACACACT ACATTCGCAT CCATAACCTG CTCCACAGCA TGCCGACGGC CCACAGCGGG 
AGCGCAGCAG AGGAAGAGGA CGGGGCGGAA GAGGAGTCAA CCCTCAAGGA TTCCTCCGAG 
AAGGATGGGT TCAGCGAGGT GGACACGGTG GCGGCGGATC CGCCGGCCCT GGAAGAGGAT 
GGAGGAGAGT TCGAGGGTGC CACTCCGGGT ATGGCACCCC CTGGCCACCT TGGGGGCCAC 
TTCCGTAGTT TGACAAATGG CGCGGGTCCT GAAGGTGACA CGCTTCCCAG CACTGGGAGT 
GAGAGCGACT CCAGCCCCCG GCAAGGCGGG GACCACAGCT GCGAGGGCTG TGACGCGTCG 
TGCTGCAGCC CCTCCTGCTA CAGCTCCTCG TGCTACAGCA CGTCCTGCTA CAGCAGCTCC 
TGCTACAGCG CCTCCTGCTA CAGCCCCTCC TGCTACAACG GCAACAACCG GTTCGCCAGC 
CACACGCGCT TCTCCTCCGT GGACAGCGCC AAGATCTCCG AGAGCACCGT CTTCTCGTCG 
CAAGACGACG AGGAAGAGGA GAACAGCGCG TTCGAGTCGG TGCCTGACTC GGTACAGAGC 
CCGGAGCTGG ACCCGGAGTC GACCAATGGT GCTGGGCCAT GGCAGGACGA GCTGGCTGCC 
CCTGGTGGGA ACCTGACAAG AACCGCTGAA GGTCTGGAGT CCCCCATGGC AGGTCCAAGC 
AATCGGAGAG AAGACTGGGA AGCTCGAATT GACAGCCATG GGCGGGTCTT TTATGTGGAC 
CATGTGAACC GTACCACCAC CTGGCAGCGT CCAACAGCAG CAGCCACCCC AGACGGGATG 
CGGAGATCCG GGTCCATCCA GCAGATGGAG CAGCTCAACA GGCGGTATCA AAATATTCAG 
CGGACCATTG CAACAGAAAG ATCTGAAGAG GATTCTGGCA GCCAAAGCTG TGAGCAAGTC 
CCAGCCGGAG GAGGTGGAGG TGGAGGGAGT GACTCAGAGG CTGAATCTCC CCAATCAAGC 
TTAGATCAGA GGAGAGAAGG GTCACTTTCT CCAGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCCGTCAA GTTCATCACC AACCCAGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGAAGAG ACGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGAG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGGA AGTCTTTTTT TGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAATGG TCGTCTTCCC AATCATCTGA CTCATCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCC 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA CCAACATGAA 
GCATTGCCAC TGGCATATAA TGACAAGATC GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAGCGTCA GCCAAGCTTG GCAAGAAATC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACCGAGGG TAATCATGGG CTTGAGAAGT TGTCCTGTGA TGCAGATCTA 
GTTATTTTGC TGAGCCTCTT TGAAGAAGAG ATTATGTCCT ATGTACCCCT GCAGGCTGCC 
TTCCACCCCG GGTATAGCTT TTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCT 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCAT ATCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTATCG GAAACTAGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGTCG GGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCC 
TATTCCCGGA AGGAGCTCCA GAGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC TCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTTGA GTACTCTGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCACCTGGAA TGGTTCAGGT TTAGTGGTCG TATCCTAGGC 
CTGGCTTTGA TCCATCAGTA CCTTCTGGAT GCTTTCTTCA CAAGGCCATT CTATAAGGCA 
CTCCTGAGGC TGCCCTGTGA TTTGAGTGAT CTGGAATATT TGGACGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGATATCC TCGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTAACAGAA AGGGAGTTAA AATCTGGAGG AGCCAACACC 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGAGGA TGGTCAAGTG GCGGGTGGAG 
CGTGGCGTGG TGCAGCAGAC TGAGGCACTG GTGCGGGGCT TCTACGAGGT TGTAGACTCG 
AGGCTCGTCT CAGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCAGAG 
ATCGACCTGA ATGACTGGCG GAATAACACT GAGTATCGGG GAGGTTACCA TGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGATTAAGA 
TTACTTCAGT TTGTCACGGG AACATCAAGT GTGCCCTATG AAGGCTTTGC TGCCCTTCGT 
GGAAGCAATG GGCTTCGGCG TTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTTCCC 
AGGGCACACA CGTGCTTCAA CCGATTGGAT CTTCCACCAT ATCCCTCATA CACTATGTTG 
TACGAAAAGC TTTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021577975.1
CDS	16..4749
Translation	MICAQSGTAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSLMNNLVESNCAEPPNDAPEPCESLRPEPTNEGTAVDSSGHQSLEHARPVEEGAGATEAGDDGRISEGPEGVGELLAPGQQDPESALSAEELAERLDLDEQVPPSLLLEDGEASANKNEPVEEEAAPESRARGEEEEKGQGEEDEEGDVPTLEHGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHNLLHSMPTAHSGSAAEEEDGAEEESTLKDSSEKDGFSEVDTVAADPPALEEDGGEFEGATPGMAPPGHLGGHFRSLTNGAGPEGDTLPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNLTRTAEGLESPMAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQVPAGGGGGGGSDSEAESPQSSLDQRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHEALPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021722300.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021722300.1

ORF Insert Sequence:

ATGATCTGTG CCCAGTCCGG GACTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC ACGAAACTCA CAGAGCCGAC GCCGGTGCAA GGAGCCACTC 
CGCTACAGCT ACAACCCTGA CCAGTTCCAC AACATGGATA TCAGGAATGG ACCTCATGAT 
GGTGTGACCA TCCCCCGCTC CACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGTTCC 
ACACTCATGG TTAGCAGCTC CTACTACTCC ATAGGGCACT CACAGGATCT GGTGATCCAC 
TGGGACATTA AGGAGGAAGT GGACGCTGGA GACTGGATTG GCATGTACCT CATAGATGAA 
GTCTTGTCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTAG AACCTGAAAC CAAGATCTGT 
TTCAAATACT ACCATGGAGT GAGTGGAGCC CTGAGAGCTA CCACCCCCAG TGTCACCGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATTGGCTCTG ATGAGACCGT CCAAGGGCAA 
GGAAGTCGGA GGCTGATCAG CTTTTCTCTC TCAGATTTCC AGGCGATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCTA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCTG CCCTTCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACA 
GTGAACCCCA TCTGGCAGGC GGAACAATTC AGTTTTGTGT CCTTGCCCAC TGATGTGCTG 
GAAATTGAGG TGAAAGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTTTTGGGA 
AAGCTGTCGA TGCCTGTCCA GAGACTCCTG GAGAGACACG CCATAGGGGA TAGAGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACTGAACCT 
GAGTCAGCAG AAATTCAGGA CAGCCTCATG AACAACCTGG TTGAAAGCAA CTGTGCGGAG 
CCTCCAAATG ATGCTCCAGA GCCCTGTGAG AGCTTGAGGC CAGAGCCAAC CAACGAGGGC 
ACTGCAGTGG ACAGTTCAGG ACACCAGAGC CTGGAGCATG CCAGGCCAGT TGAGGAAGGG 
GCAGGTGCCA CTGAGGCAGG AGATGATGGC AGGATCTCTG AGGGACCTGA AGGGGTTGGG 
GAGCTTCTGG CCCCAGGACA ACAGGACCCC GAGTCTGCCC TGAGTGCAGA AGAACTAGCC 
GAGAGGCTCG ACCTGGATGA ACAGGTACCA CCATCGCTAC TTCTGGAAGA TGGTGAGGCT 
TCCGCCAACA AGAATGAGCC GGTGGAGGAG GAGGCAGCAC CCGAGAGCCG GGCCAGGGGG 
GAGGAGGAAG AGAAAGGCCA GGGTGAAGAG GATGAGGAGG GGGATGTGCC TACCCTGGAG 
CACGGAGAGG GCAGACTGCA GCTGCGAGCC TCAGTGAAGA GAAAAAGCAG ACCATGCTCT 
TTGCCTGTGT CCGAGCTGGA GACCGTGATC GCGTCGGCCT GCGGGGACCC CGAGACCCCG 
CGGACACACT ACATTCGCAT CCATAACCTG CTCCACAGCA TGCCGACGGC CCACAGCGGG 
AGCGCAGCAG AGGAAGAGGA CGGGGCGGAA GAGGAGTCAA CCCTCAAGGA TTCCTCCGAG 
AAGGATGGGT TCAGCGAGGT GGACACGGTG GCGGCGGATC CGCCGGCCCT GGAAGAGGAT 
GGAGGAGAGT TCGAGGGTGC CACTCCGGGT ATGGCACCCC CTGGCCACCT TGGGGGCCAC 
TTCCGTAGTT TGACAAATGG CGCGGGTCCT GAAGGTGACA CGCTTCCCAG CACTGGGAGT 
GAGAGCGACT CCAGCCCCCG GCAAGGCGGG GACCACAGCT GCGAGGGCTG TGACGCGTCG 
TGCTGCAGCC CCTCCTGCTA CAGCTCCTCG TGCTACAGCA CGTCCTGCTA CAGCAGCTCC 
TGCTACAGCG CCTCCTGCTA CAGCCCCTCC TGCTACAACG GCAACAACCG GTTCGCCAGC 
CACACGCGCT TCTCCTCCGT GGACAGCGCC AAGATCTCCG AGAGCACCGT CTTCTCGTCG 
CAAGACGACG AGGAAGAGGA GAACAGCGCG TTCGAGTCGG TGCCTGACTC GGTACAGAGC 
CCGGAGCTGG ACCCGGAGTC GACCAATGGT GCTGGGCCAT GGCAGGACGA GCTGGCTGCC 
CCTGGTGGGA ACCTGACAAG AACCGCTGAA GGTCTGGAGT CCCCCATGGC AGGTCCAAGC 
AATCGGAGAG AAGACTGGGA AGCTCGAATT GACAGCCATG GGCGGGTCTT TTATGTGGAC 
CATGTGAACC GTACCACCAC CTGGCAGCGT CCAACAGCAG CAGCCACCCC AGACGGGATG 
CGGAGATCCG GGTCCATCCA GCAGATGGAG CAGCTCAACA GGCGGTATCA AAATATTCAG 
CGGACCATTG CAACAGAAAG ATCTGAAGAG GATTCTGGCA GCCAAAGCTG TGAGCAAGTC 
CCAGCCGGAG GAGGTGGAGG TGGAGGGAGT GACTCAGAGG CTGAATCTCC CCAATCAAGC 
TTAGATCAGA GGAGAGAAGG GTCACTTTCT CCAGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCCGTCAA GTTCATCACC AACCCAGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGAAGAG ACGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGAG GCTGGGAGAT CAAAACAGAC 
CAGCAGGGGA AGTCTTTTTT TGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAATGG TCGTCTTCCC AATCATCTGA CTCATCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCCTCC 
TTACTGGCCA GGCCAGGACA CAGCCTAGTA GCTGCTATTC GAAGCCAACA CCAACATGAA 
GCATTGCCAC TGGCATATAA TGACAAGATC GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAGCGTCA GCCAAGCTTG GCAAGAAATC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACCGAGGG TAATCATGGG CTTGAGAAGT TGTCCTGTGA TGCAGATCTA 
GTTATTTTGC TGAGCCTCTT TGAAGAAGAG ATTATGTCCT ATGTACCCCT GCAGGCTGCC 
TTCCACCCCG GGTATAGCTT TTCTCCCCGC TGTTCACCCT GTTCTTCACC TCAGAACTCT 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCACCAT ATCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTATCG GAAACTAGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGTCG GGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCC 
TATTCCCGGA AGGAGCTCCA GAGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGTCCTTC TCGAGAGTTC TTCTTCCTTT TGTCTCAGGA GCTCTTCAAC 
CCTTACTACG GACTCTTTGA GTACTCTGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCTGCGT TTGTAGAAAA CCACCTGGAA TGGTTCAGGT TTAGTGGTCG TATCCTAGGC 
CTGGCTTTGA TCCATCAGTA CCTTCTGGAT GCTTTCTTCA CAAGGCCATT CTATAAGGCA 
CTCCTGAGGC TGCCCTGTGA TTTGAGTGAT CTGGAATATT TGGACGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGATATCC TCGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTAACAGAA AGGGAGTTAA AATCTGGAGG AGCCAACACC 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGAGGA TGGTCAAGTG GCGGGTGGAG 
CGTGGCGTGG TGCAGCAGAC TGAGGCACTG GTGCGGGGCT TCTACGAGGT TGTAGACTCG 
AGGCTCGTCT CAGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCAGAG 
ATCGACCTGA ATGACTGGCG GAATAACACT GAGTATCGGG GAGGTTACCA TGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGATTAAGA 
TTACTTCAGT TTGTCACGGG AACATCAAGT GTGCCCTATG AAGGCTTTGC TGCCCTTCGT 
GGAAGCAATG GGCTTCGGCG TTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTTCCC 
AGGGCACACA CGTGCTTCAA CCGATTGGAT CTTCCACCAT ATCCCTCATA CACTATGTTG 
TACGAAAAGC TTTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW1 cDNA ORF clone, Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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