SPTA1 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTA1
Entrez Gene ID	101097298
Full Name	spectrin alpha, erythrocytic 1
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019822344.1	XP_019677903.1	spectrin alpha chain, erythrocytic 1 isoform X1
XM_019822344.2	XP_019677903.2	spectrin alpha chain, erythrocytic 1 isoform X1
XM_019822345.2	XP_019677904.2	spectrin alpha chain, erythrocytic 1 isoform X2
XM_019822345.1	XP_019677904.1	spectrin alpha chain, erythrocytic 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SPTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb56758	XM_019822344.1 Latest version!	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb56759	XM_019822345.1 Latest version!	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb67911	XM_019822344.2 Latest version!	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb67912	XM_019822345.2 Latest version!	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTA1 ( XM_019822344.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677903.1 Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb56758
Clone ID Related Accession (Same CDS sequence)	XM_019822344.1
Accession Version	XM_019822344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	spectrin alpha chain, erythrocytic 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CATCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGTG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT TGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACATGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGACAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGCT GAGGAGCTGT TCATGGAATT TGCACACAAG 
GCTTCCGCTT TCAACCACTG GTGTGAGAAC GCCAAGGAGG ACCTGTCAGA GCCTGTGCAC 
TGTGTTTCCC TGAACGAGAT ACGGAAACTG CAGAAGGACC AGGAGGCCTT CCTGGCCTCC 
TTGGTGGGGG CCCAGTCAGA CTTCAACTAT TTGCTGGAGC TGGACCAGCA GATTAAGGCC 
TTAGACGTGC CTTCCAGCCC TTACACCTGG TTGACGATGG AGGTGCTGGA GAAGGTCTGG 
AAGCACCTAC ATGACATCAT CAAGGAACGG GAGCAGGAGC TACAAAAAGA GGAGGAGAGA 
CAGGTCAAGA ACTTTGAGAT GTGCCAGGAG TTTGAGCAGA ACGCCAGTGC CTTCCTTCAG 
TGGATCTTGG AGACCAGGGC TTATTTTCTG GATGGGTCTT TGCTCAAAGA GACGGGAACC 
CTGGAATCCC AGTTGGAAGC AAACAAACGG AAACAGAAGG AGATTCAGGC CATGAAGCGC 
CAGCTGACCA AGATTGAGGA TCTGGGAGAC AACTTAGAAG AGGCTCTGGT CCTGGACATC 
AAGTACAGCA CCATCGGGCT GGCCCAGCAG TGGGACCAGC TCCACCAGCT GGGAACGCGG 
ATGCAGCACA ACCTGGAACA ACAGATCCAA GCCAAAGACA CCATAGGTGT GAGTGAGGAG 
ACATTGAAGG AGTTCAGCAC AACCTATAAA CATTTTGATG AGAATCTGAC GGGGCGCTTG 
AGTCACAAAG ATTTCCGGTC CTGTCTCAGA GGACTCAACT ACTACCTGCC CATGGTGGAG 
GACGATGAAC CTGAGCCCAA GTTTGAGAAG TTTCTGGATG TCGTCGATCC AGGGAGGAAG 
GGCTACATCA CCCTGGAGGA CTACACCTCA TTCCTGATCG ACAAGGAGTC AGAGAACATC 
AAGTCCAGCG ATGAAATAGA GAATGGCTTC CAGGCCCTGG CAGAGGGCAA GGCCTATATT 
ACCAAAGACG ACATGAAGCA GGCCCTAACC CCAGAACAAG TGCAGTTCTG TGCATCACAT 
ATGCAAGAAT ACGTGGACCC GCGAGGCCAA AGACACCTTG CTGGTTATGA CTACGTGGGC 
TTCACCAGTT CCTACTTTGG CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677903.1
CDS	290..7516
Translation	METSGPKVLETAEAIQERRREVLTRYQRFKELVAERGQKLEESYHYQVFRRDADDLEKWVLEKLKIADDKSYEDLTNIQGKYRKHESLEAEIQAKSRVIPELEEIRETRFAEGHFAHEDTKAHLEQLRTLWDLLLELTRERGALLLRALRLQQYLQECADTLEWIGDKEAIVTSAELGEDWERTEVLHKKFEEFQADLETQKRKLDGMNQYANQCAEEKHPDLPLIKLKQDEVNTAWKRLLSLAGQRQETLANAADLQRFKRDVTEAIQWIKEKEPQLTSEDYGKDLISSEALFHSHKGLERNLAVMQDKVKELSAKADRLMLSHPSDASQIQQMKEELVSNWEHIRALAASRYEKLQASYWYQRFLSDYEELSGWMKEKSALINADELPTDVAGGEALLDRHGQHKHEIDSYDDRFQSAEETGRELLDVGHEASDEVLEKMTILANDWAALRGLWDQRKHQYEQCLYLHLFYRDSEQVDSWMSRQEAFLEDEDLGNSLGSVEALLQKHDDFEEAFNAQEEKITTLDNNATKLIDNDHYDSEKIAALRDSLLSRRDALRARAAMRRRLLEDSWLLQKLYEDSDDLKNWVNKKKKLADDEDYKDTQNLKSRVKKQEDFKKELQDNQRLLNTLEKTGQEMIEANHYAADKVTARVAEVVSLWKELLAAMEQKGTQLHDANQELQFKNNAEDLEHWLDKAEEQAASEDYGKGLADIQSLLRKHGLLESAVATRQDQVDSLTDRAAYFEEIGHPNAGDIQARRESLVSRFEALKEPMATRKKKLIDFLHLLQLFRDTEDEEAWIEETEPSVASTYLGKDLIASKKLLNRHQVIQDDIANHEPHVRMITERGNKMVEEGHFAAEDVASKVKALNDNMESLQARAARRQNDLEANVQFQQYLADLHEAEAWIQEKAPIVDNTNYGADEEAAGALLKKHEAFLVDLSAFGNSMQALRDQAEACQQQQAAPVEGAAREQRVVALYDFEARSNREVTMKKDDVLTLLSSINKDWWKVEAGDHQGFVPAVYVRKLAHDEFPTLPQRRREEPGSITERQEQIEKQYRSLLDRAEERRCRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQTDLKANEPRLRDINKVADDLLFEKLLTPEGAQLRQELNNRWDSLQRLAEEQRQLLGSAHAVQMFHREADDTKEQIEKKLQALRAADPGSDLFSVQALQRQHEVFERDLMPLEEKVTMLRETAERLSESHPDAAGDLQNQGMELKETWDDLLGCTEDRKHDLNEARQFYTFLNRARDLENWMSGIGGMVSSEELAEDLTGTEILLERHSGHYADMEAEAPTFQALEEFGGELVRSGHRDSPEIQEKLEAVRQEKGDLEKAWDQRKKMLHQCLELQLFHGSCDQAESWMMARENFLRSERAGPLDSLEALMKKRDDLEKAINAQDKKITELGNVAERLIADDHYAKEEIAARLQRVLDRWKALKKLLVAEQTKLGDYADVKQFYRDLEDLEEWIGEMLPTACDESYKDPTNIQRKYLKHKAFENEVKGRTEQVKGVLTLGNSLIERQACDGNEETVRKQLQELQRQWDYLLEKTADKGEKLDEASRQQRFNTSIQDFKFWLSEAETLLAMKDQARDLASAGNLLKKHQLLETEMSAREDALKDLNESATHLLSSGTFNVDQIVEQRDNVNERFLNVRNLAAAHHETLKEAYALFQFFQDLDDEESWIEEKLLRVRSQDYGRDLQGVQNLLRKHKRLAGELQAHEPTIQNILDAAERLGDKAAVGREEIQERLTHLFQQWEKLKELASTRGLRLEESLEYLKFMENAEEEEAWISEKEAMVARGGSGDTLAATQSLLKKHKALENDFAVHETRVQDVCAQGEDILNKEKSQHMEKVATKIQTLTEKIPSLAKAMAVWKLQLEDDYDFQQFNWKADVVEAWIAEKEASLKTNGNDTDLTASLALLAKQDTLDASLQSFQQDRLSEITDLKDQLVAAQHSQTKAIEERHAALLRRWGELLEASAAHRQNLLEKQLPLKKAEELFMEFAHKASAFNHWCENAKEDLSEPVHCVSLNEIRKLQKDQEAFLASLVGAQSDFNYLLELDQQIKALDVPSSPYTWLTMEVLEKVWKHLHDIIKEREQELQKEEERQVKNFEMCQEFEQNASAFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIEDLGDNLEEALVLDIKYSTIGLAQQWDQLHQLGTRMQHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLSHKDFRSCLRGLNYYLPMVEDDEPEPKFEKFLDVVDPGRKGYITLEDYTSFLIDKESENIKSSDEIENGFQALAEGKAYITKDDMKQALTPEQVQFCASHMQEYVDPRGQRHLAGYDYVGFTSSYFGN

Target ORF information:

RefSeq Version	XM_019822344.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019822344.1

ORF Insert Sequence:

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CATCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGTG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT TGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACATGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGACAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGCT GAGGAGCTGT TCATGGAATT TGCACACAAG 
GCTTCCGCTT TCAACCACTG GTGTGAGAAC GCCAAGGAGG ACCTGTCAGA GCCTGTGCAC 
TGTGTTTCCC TGAACGAGAT ACGGAAACTG CAGAAGGACC AGGAGGCCTT CCTGGCCTCC 
TTGGTGGGGG CCCAGTCAGA CTTCAACTAT TTGCTGGAGC TGGACCAGCA GATTAAGGCC 
TTAGACGTGC CTTCCAGCCC TTACACCTGG TTGACGATGG AGGTGCTGGA GAAGGTCTGG 
AAGCACCTAC ATGACATCAT CAAGGAACGG GAGCAGGAGC TACAAAAAGA GGAGGAGAGA 
CAGGTCAAGA ACTTTGAGAT GTGCCAGGAG TTTGAGCAGA ACGCCAGTGC CTTCCTTCAG 
TGGATCTTGG AGACCAGGGC TTATTTTCTG GATGGGTCTT TGCTCAAAGA GACGGGAACC 
CTGGAATCCC AGTTGGAAGC AAACAAACGG AAACAGAAGG AGATTCAGGC CATGAAGCGC 
CAGCTGACCA AGATTGAGGA TCTGGGAGAC AACTTAGAAG AGGCTCTGGT CCTGGACATC 
AAGTACAGCA CCATCGGGCT GGCCCAGCAG TGGGACCAGC TCCACCAGCT GGGAACGCGG 
ATGCAGCACA ACCTGGAACA ACAGATCCAA GCCAAAGACA CCATAGGTGT GAGTGAGGAG 
ACATTGAAGG AGTTCAGCAC AACCTATAAA CATTTTGATG AGAATCTGAC GGGGCGCTTG 
AGTCACAAAG ATTTCCGGTC CTGTCTCAGA GGACTCAACT ACTACCTGCC CATGGTGGAG 
GACGATGAAC CTGAGCCCAA GTTTGAGAAG TTTCTGGATG TCGTCGATCC AGGGAGGAAG 
GGCTACATCA CCCTGGAGGA CTACACCTCA TTCCTGATCG ACAAGGAGTC AGAGAACATC 
AAGTCCAGCG ATGAAATAGA GAATGGCTTC CAGGCCCTGG CAGAGGGCAA GGCCTATATT 
ACCAAAGACG ACATGAAGCA GGCCCTAACC CCAGAACAAG TGCAGTTCTG TGCATCACAT 
ATGCAAGAAT ACGTGGACCC GCGAGGCCAA AGACACCTTG CTGGTTATGA CTACGTGGGC 
TTCACCAGTT CCTACTTTGG CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_019822345.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677904.1 Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

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CloneID	OFb56759
Clone ID Related Accession (Same CDS sequence)	XM_019822345.1
Accession Version	XM_019822345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	spectrin alpha chain, erythrocytic 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CATCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGTG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT TGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACATGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGACAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGAA CGGGAGCAGG AGCTACAAAA AGAGGAGGAG 
AGACAGGTCA AGAACTTTGA GATGTGCCAG GAGTTTGAGC AGAACGCCAG TGCCTTCCTT 
CAGTGGATCT TGGAGACCAG GGCTTATTTT CTGGATGGGT CTTTGCTCAA AGAGACGGGA 
ACCCTGGAAT CCCAGTTGGA AGCAAACAAA CGGAAACAGA AGGAGATTCA GGCCATGAAG 
CGCCAGCTGA CCAAGATTGA GGATCTGGGA GACAACTTAG AAGAGGCTCT GGTCCTGGAC 
ATCAAGTACA GCACCATCGG GCTGGCCCAG CAGTGGGACC AGCTCCACCA GCTGGGAACG 
CGGATGCAGC ACAACCTGGA ACAACAGATC CAAGCCAAAG ACACCATAGG TGTGAGTGAG 
GAGACATTGA AGGAGTTCAG CACAACCTAT AAACATTTTG ATGAGAATCT GACGGGGCGC 
TTGAGTCACA AAGATTTCCG GTCCTGTCTC AGAGGACTCA ACTACTACCT GCCCATGGTG 
GAGGACGATG AACCTGAGCC CAAGTTTGAG AAGTTTCTGG ATGTCGTCGA TCCAGGGAGG 
AAGGGCTACA TCACCCTGGA GGACTACACC TCATTCCTGA TCGACAAGGA GTCAGAGAAC 
ATCAAGTCCA GCGATGAAAT AGAGAATGGC TTCCAGGCCC TGGCAGAGGG CAAGGCCTAT 
ATTACCAAAG ACGACATGAA GCAGGCCCTA ACCCCAGAAC AAGTGCAGTT CTGTGCATCA 
CATATGCAAG AATACGTGGA CCCGCGAGGC CAAAGACACC TTGCTGGTTA TGACTACGTG 
GGCTTCACCA GTTCCTACTT TGGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677904.1
CDS	290..7219
Translation	METSGPKVLETAEAIQERRREVLTRYQRFKELVAERGQKLEESYHYQVFRRDADDLEKWVLEKLKIADDKSYEDLTNIQGKYRKHESLEAEIQAKSRVIPELEEIRETRFAEGHFAHEDTKAHLEQLRTLWDLLLELTRERGALLLRALRLQQYLQECADTLEWIGDKEAIVTSAELGEDWERTEVLHKKFEEFQADLETQKRKLDGMNQYANQCAEEKHPDLPLIKLKQDEVNTAWKRLLSLAGQRQETLANAADLQRFKRDVTEAIQWIKEKEPQLTSEDYGKDLISSEALFHSHKGLERNLAVMQDKVKELSAKADRLMLSHPSDASQIQQMKEELVSNWEHIRALAASRYEKLQASYWYQRFLSDYEELSGWMKEKSALINADELPTDVAGGEALLDRHGQHKHEIDSYDDRFQSAEETGRELLDVGHEASDEVLEKMTILANDWAALRGLWDQRKHQYEQCLYLHLFYRDSEQVDSWMSRQEAFLEDEDLGNSLGSVEALLQKHDDFEEAFNAQEEKITTLDNNATKLIDNDHYDSEKIAALRDSLLSRRDALRARAAMRRRLLEDSWLLQKLYEDSDDLKNWVNKKKKLADDEDYKDTQNLKSRVKKQEDFKKELQDNQRLLNTLEKTGQEMIEANHYAADKVTARVAEVVSLWKELLAAMEQKGTQLHDANQELQFKNNAEDLEHWLDKAEEQAASEDYGKGLADIQSLLRKHGLLESAVATRQDQVDSLTDRAAYFEEIGHPNAGDIQARRESLVSRFEALKEPMATRKKKLIDFLHLLQLFRDTEDEEAWIEETEPSVASTYLGKDLIASKKLLNRHQVIQDDIANHEPHVRMITERGNKMVEEGHFAAEDVASKVKALNDNMESLQARAARRQNDLEANVQFQQYLADLHEAEAWIQEKAPIVDNTNYGADEEAAGALLKKHEAFLVDLSAFGNSMQALRDQAEACQQQQAAPVEGAAREQRVVALYDFEARSNREVTMKKDDVLTLLSSINKDWWKVEAGDHQGFVPAVYVRKLAHDEFPTLPQRRREEPGSITERQEQIEKQYRSLLDRAEERRCRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQTDLKANEPRLRDINKVADDLLFEKLLTPEGAQLRQELNNRWDSLQRLAEEQRQLLGSAHAVQMFHREADDTKEQIEKKLQALRAADPGSDLFSVQALQRQHEVFERDLMPLEEKVTMLRETAERLSESHPDAAGDLQNQGMELKETWDDLLGCTEDRKHDLNEARQFYTFLNRARDLENWMSGIGGMVSSEELAEDLTGTEILLERHSGHYADMEAEAPTFQALEEFGGELVRSGHRDSPEIQEKLEAVRQEKGDLEKAWDQRKKMLHQCLELQLFHGSCDQAESWMMARENFLRSERAGPLDSLEALMKKRDDLEKAINAQDKKITELGNVAERLIADDHYAKEEIAARLQRVLDRWKALKKLLVAEQTKLGDYADVKQFYRDLEDLEEWIGEMLPTACDESYKDPTNIQRKYLKHKAFENEVKGRTEQVKGVLTLGNSLIERQACDGNEETVRKQLQELQRQWDYLLEKTADKGEKLDEASRQQRFNTSIQDFKFWLSEAETLLAMKDQARDLASAGNLLKKHQLLETEMSAREDALKDLNESATHLLSSGTFNVDQIVEQRDNVNERFLNVRNLAAAHHETLKEAYALFQFFQDLDDEESWIEEKLLRVRSQDYGRDLQGVQNLLRKHKRLAGELQAHEPTIQNILDAAERLGDKAAVGREEIQERLTHLFQQWEKLKELASTRGLRLEESLEYLKFMENAEEEEAWISEKEAMVARGGSGDTLAATQSLLKKHKALENDFAVHETRVQDVCAQGEDILNKEKSQHMEKVATKIQTLTEKIPSLAKAMAVWKLQLEDDYDFQQFNWKADVVEAWIAEKEASLKTNGNDTDLTASLALLAKQDTLDASLQSFQQDRLSEITDLKDQLVAAQHSQTKAIEERHAALLRRWGELLEASAAHRQNLLEKQLPLKKEREQELQKEEERQVKNFEMCQEFEQNASAFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIEDLGDNLEEALVLDIKYSTIGLAQQWDQLHQLGTRMQHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLSHKDFRSCLRGLNYYLPMVEDDEPEPKFEKFLDVVDPGRKGYITLEDYTSFLIDKESENIKSSDEIENGFQALAEGKAYITKDDMKQALTPEQVQFCASHMQEYVDPRGQRHLAGYDYVGFTSSYFGN

Target ORF information:

RefSeq Version	XM_019822345.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019822345.1

ORF Insert Sequence:

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CATCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGTG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT TGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACATGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGACAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGAA CGGGAGCAGG AGCTACAAAA AGAGGAGGAG 
AGACAGGTCA AGAACTTTGA GATGTGCCAG GAGTTTGAGC AGAACGCCAG TGCCTTCCTT 
CAGTGGATCT TGGAGACCAG GGCTTATTTT CTGGATGGGT CTTTGCTCAA AGAGACGGGA 
ACCCTGGAAT CCCAGTTGGA AGCAAACAAA CGGAAACAGA AGGAGATTCA GGCCATGAAG 
CGCCAGCTGA CCAAGATTGA GGATCTGGGA GACAACTTAG AAGAGGCTCT GGTCCTGGAC 
ATCAAGTACA GCACCATCGG GCTGGCCCAG CAGTGGGACC AGCTCCACCA GCTGGGAACG 
CGGATGCAGC ACAACCTGGA ACAACAGATC CAAGCCAAAG ACACCATAGG TGTGAGTGAG 
GAGACATTGA AGGAGTTCAG CACAACCTAT AAACATTTTG ATGAGAATCT GACGGGGCGC 
TTGAGTCACA AAGATTTCCG GTCCTGTCTC AGAGGACTCA ACTACTACCT GCCCATGGTG 
GAGGACGATG AACCTGAGCC CAAGTTTGAG AAGTTTCTGG ATGTCGTCGA TCCAGGGAGG 
AAGGGCTACA TCACCCTGGA GGACTACACC TCATTCCTGA TCGACAAGGA GTCAGAGAAC 
ATCAAGTCCA GCGATGAAAT AGAGAATGGC TTCCAGGCCC TGGCAGAGGG CAAGGCCTAT 
ATTACCAAAG ACGACATGAA GCAGGCCCTA ACCCCAGAAC AAGTGCAGTT CTGTGCATCA 
CATATGCAAG AATACGTGGA CCCGCGAGGC CAAAGACACC TTGCTGGTTA TGACTACGTG 
GGCTTCACCA GTTCCTACTT TGGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_019822344.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677903.2 Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

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CloneID	OFb67911
Clone ID Related Accession (Same CDS sequence)	XM_019822344.2
Accession Version	XM_019822344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin alpha chain, erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019822344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CGTCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGCG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT CGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACACGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGATAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGCT GAGGAGCTGT TCATGGAATT TGCACACAAG 
GCTTCCGCTT TCAACCACTG GTGTGAGAAC GCCAAGGAGG ACCTGTCAGA GCCTGTGCAC 
TGTGTTTCCC TGAACGAGAT ACGGAAACTG CAGAAGGACC AGGAGGCCTT CCTGGCCTCC 
TTGGTGGGGG CCCAGTCGGA CTTCAGCTAT TTGCTGGAGC TGGACCAGCA GATTAAGGCC 
TTAGACGTGC CTTCCAGCCC TTACACCTGG TTGACGATGG AGGTGCTGGA GAATGTCTGG 
AAGCATCTAC ATGGCATCAT CAAGGAACGG GAGCAGGAGC TACAAAAAGA GGAGGAGAGA 
CAGGTCAAGA ACTTTGAGAT GTGCCAGGAG TTTGAGCAGA ATGCCAGTGC CTTCCTTCAG 
TGGATCTTGG AGACCAGGGC TTATTTTCTG GATGGGTCTT TGCTCAAAGA GACGGGAACC 
CTGGAATCCC AGTTGGAAGC AAACAAACGG AAACAGAAGG AGATTCAGGC CATGAAGCGC 
CAGCTGACCA AGATTGAGGA TCTGGGAGAC AACTTAGAAG AGGCTCTGGT CCTGGACATC 
AAGTACAGCA CCATCGGGCT GGCCCAGCAG TGGGACCAGC TCCACCAGCT GGGAACACGG 
ATGCAGCACA ACCTGGAACA ACAGATCCAA GCCAAAGACA CCATAGGTGT GAGTGAGGAG 
ACATTGAAGG AGTTCAGCAC AACCTATAAA CATTTTGATG AGAATCTGAC GGGGCGCTTG 
AGTCACAAAG ATTTCCGGTC CTGCCTCAGA GGACTCAACT ACTACCTGCC CATGGTGGAG 
GACGATGAAC CTGAGCCCAA GTTTGAGAAG TTCCTGGATG TCGTCGATCC AGGGAGGAAG 
GGCTACATCA CCCTGGAGGA CTACACCTCA TTCCTGATCG ACAAGGAGTC AGAGAACATC 
AAGTCCAGCG ATGAAATAGA GAATGGCTTC CAGGCCCTGG CAGAGGGCAA GGCCTATATT 
ACCAAAGACG ACATGAAGCA GGCCCTAACC CCAGAACAAG TGCAGTTCTG TGCATCACAT 
ATGCAAGAAT ACGTGGACCC GCGAGGCCAA AGACACCTTG CTGGTTATGA CTACGTGGGC 
TTCACCAGTT CCTACTTTGG CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677903.2
CDS	290..7516
Translation	METSGPKVLETAEAIQERRREVLTRYQRFKELVAERGQKLEESYHYQVFRRDADDLEKWVLEKLKIADDKSYEDLTNIQGKYRKHESLEAEIQAKSRVIPELEEIRETRFAEGHFAHEDTKAHLEQLRTLWDLLLELTRERGALLLRALRLQQYLQECADTLEWIGDKEAIVTSAELGEDWERTEVLHKKFEEFQADLETQKRKLDGMNQYANQCAEEKHPDLPLIKLKQDEVNTAWKRLLSLAGQRQETLANAADLQRFKRDVTEAVQWIKEKEPQLTSEDYGKDLISSEALFHSHKGLERNLAVMQDKVKELSAKADRLMLSHPSDASQIQQMKEELVSNWEHIRALAASRYEKLQASYWYQRFLSDYEELSGWMKEKSALINADELPTDVAGGEALLDRHGQHKHEIDSYDDRFQSAEETGRELLDVGHEASDEVLEKMTILANDWAALRGLWDQRKHQYEQCLYLHLFYRDSEQVDSWMSRQEAFLEDEDLGNSLGSVEALLQKHDDFEEAFNAQEEKITTLDNNATKLIDNDHYDSEKIAALRDSLLSRRDALRARAAMRRRLLEDSWLLQKLYEDSDDLKNWVNKKKKLADDEDYKDTQNLKSRVKKQEDFKKELQDNQRLLNTLEKTGQEMIEANHYAADKVTARVAEVVSLWKELLAAMEQKGTQLHDANQELQFKNNAEDLEHWLDKAEEQAASEDYGKGLADIQSLLRKHGLLESAVATRQDQVDSLTDRAAYFEEIGHPNAGDIQARRESLVSRFEALKEPMATRKKKLIDFLHLLQLFRDTEDEEAWIEETEPSVASTYLGKDLIASKKLLNRHQVIQDDIANHEPHVRMITERGNKMVEEGHFAAEDVASKVKALNDNMESLQARAARRQNDLEANVQFQQYLADLHEAEAWIQEKAPIVDNTNYGADEEAAGALLKKHEAFLVDLSAFGNSMQALRDQAEACQQQQAAPVEGAAREQRVVALYDFEARSNREVTMKKDDVLTLLSSINKDWWKVEAGDHQGFVPAVYVRKLAHDEFPTLPQRRREEPGSITERQEQIEKQYRSLLDRAEERRCRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQTDLKANEPRLRDINKVADDLLFEKLLTPEGAQLRQELNNRWDSLQRLAEEQRQLLGSAHAVQMFHREADDTKEQIEKKLQALRAADPGSDLFSVQALQRQHEVFERDLMPLEEKVTMLRETAERLSESHPDAAGDLQNQGMELKETWDDLLGCTEDRKHDLNEARQFYTFLNRARDLENWMSGIGGMVSSEELAEDLTGTEILLERHSGHYADMEAEAPTFQALEEFGGELVRSGHRDSPEIQEKLEAVRQEKGDLEKAWDQRKKMLHQCLELQLFHGSCDQAESWMMARENFLRSERAGPLDSLEALMKKRDDLEKAINAQDKKITELGNVAERLIADDHYAKEEIAARLQRVLDRWKALKKLLVAEQTKLGDYADVKQFYRDLEDLEEWIGEMLPTACDESYKDPTNIQRKYLKHKAFENEVKGRTEQVKGVLTLGNSLIERQACDGNEETVRKQLQELQRQWDYLLEKTADKGEKLDEASRQQRFNTSIQDFKFWLSEAETLLAMKDQARDLASAGNLLKKHQLLETEMSAREDALKDLNESATHLLSSGTFNVDQIVEQRDNVNERFLNVRNLAAAHHETLKEAYALFQFFQDLDDEESWIEEKLLRVRSQDYGRDLQGVQNLLRKHKRLAGELQAHEPTIQNILDAAERLGDKAAVGREEIQERLTHLFQQWEKLKELASTRGLRLEESLEYLKFMENAEEEEAWISEKEAMVARGGSGDTLAATQSLLKKHKALENDFAVHETRVQDVCAQGEDILNKEKSQHTEKVATKIQTLTEKIPSLAKAMAVWKLQLEDDYDFQQFNWKADVVEAWIAEKEASLKTNGNDTDLTASLALLAKQDTLDASLQSFQQDRLSEITDLKDQLVAAQHSQTKAIEERHAALLRRWGELLEASAAHRQNLLEKQLPLKKAEELFMEFAHKASAFNHWCENAKEDLSEPVHCVSLNEIRKLQKDQEAFLASLVGAQSDFSYLLELDQQIKALDVPSSPYTWLTMEVLENVWKHLHGIIKEREQELQKEEERQVKNFEMCQEFEQNASAFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIEDLGDNLEEALVLDIKYSTIGLAQQWDQLHQLGTRMQHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLSHKDFRSCLRGLNYYLPMVEDDEPEPKFEKFLDVVDPGRKGYITLEDYTSFLIDKESENIKSSDEIENGFQALAEGKAYITKDDMKQALTPEQVQFCASHMQEYVDPRGQRHLAGYDYVGFTSSYFGN

Target ORF information:

RefSeq Version	XM_019822344.2
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019822344.2

ORF Insert Sequence:

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CGTCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGCG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT CGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACACGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGATAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGCT GAGGAGCTGT TCATGGAATT TGCACACAAG 
GCTTCCGCTT TCAACCACTG GTGTGAGAAC GCCAAGGAGG ACCTGTCAGA GCCTGTGCAC 
TGTGTTTCCC TGAACGAGAT ACGGAAACTG CAGAAGGACC AGGAGGCCTT CCTGGCCTCC 
TTGGTGGGGG CCCAGTCGGA CTTCAGCTAT TTGCTGGAGC TGGACCAGCA GATTAAGGCC 
TTAGACGTGC CTTCCAGCCC TTACACCTGG TTGACGATGG AGGTGCTGGA GAATGTCTGG 
AAGCATCTAC ATGGCATCAT CAAGGAACGG GAGCAGGAGC TACAAAAAGA GGAGGAGAGA 
CAGGTCAAGA ACTTTGAGAT GTGCCAGGAG TTTGAGCAGA ATGCCAGTGC CTTCCTTCAG 
TGGATCTTGG AGACCAGGGC TTATTTTCTG GATGGGTCTT TGCTCAAAGA GACGGGAACC 
CTGGAATCCC AGTTGGAAGC AAACAAACGG AAACAGAAGG AGATTCAGGC CATGAAGCGC 
CAGCTGACCA AGATTGAGGA TCTGGGAGAC AACTTAGAAG AGGCTCTGGT CCTGGACATC 
AAGTACAGCA CCATCGGGCT GGCCCAGCAG TGGGACCAGC TCCACCAGCT GGGAACACGG 
ATGCAGCACA ACCTGGAACA ACAGATCCAA GCCAAAGACA CCATAGGTGT GAGTGAGGAG 
ACATTGAAGG AGTTCAGCAC AACCTATAAA CATTTTGATG AGAATCTGAC GGGGCGCTTG 
AGTCACAAAG ATTTCCGGTC CTGCCTCAGA GGACTCAACT ACTACCTGCC CATGGTGGAG 
GACGATGAAC CTGAGCCCAA GTTTGAGAAG TTCCTGGATG TCGTCGATCC AGGGAGGAAG 
GGCTACATCA CCCTGGAGGA CTACACCTCA TTCCTGATCG ACAAGGAGTC AGAGAACATC 
AAGTCCAGCG ATGAAATAGA GAATGGCTTC CAGGCCCTGG CAGAGGGCAA GGCCTATATT 
ACCAAAGACG ACATGAAGCA GGCCCTAACC CCAGAACAAG TGCAGTTCTG TGCATCACAT 
ATGCAAGAAT ACGTGGACCC GCGAGGCCAA AGACACCTTG CTGGTTATGA CTACGTGGGC 
TTCACCAGTT CCTACTTTGG CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_019822345.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677904.2 Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

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CloneID	OFb67912
Clone ID Related Accession (Same CDS sequence)	XM_019822345.2
Accession Version	XM_019822345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin alpha chain, erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019822345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CGTCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGCG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT CGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACACGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGATAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGAA CGGGAGCAGG AGCTACAAAA AGAGGAGGAG 
AGACAGGTCA AGAACTTTGA GATGTGCCAG GAGTTTGAGC AGAATGCCAG TGCCTTCCTT 
CAGTGGATCT TGGAGACCAG GGCTTATTTT CTGGATGGGT CTTTGCTCAA AGAGACGGGA 
ACCCTGGAAT CCCAGTTGGA AGCAAACAAA CGGAAACAGA AGGAGATTCA GGCCATGAAG 
CGCCAGCTGA CCAAGATTGA GGATCTGGGA GACAACTTAG AAGAGGCTCT GGTCCTGGAC 
ATCAAGTACA GCACCATCGG GCTGGCCCAG CAGTGGGACC AGCTCCACCA GCTGGGAACA 
CGGATGCAGC ACAACCTGGA ACAACAGATC CAAGCCAAAG ACACCATAGG TGTGAGTGAG 
GAGACATTGA AGGAGTTCAG CACAACCTAT AAACATTTTG ATGAGAATCT GACGGGGCGC 
TTGAGTCACA AAGATTTCCG GTCCTGCCTC AGAGGACTCA ACTACTACCT GCCCATGGTG 
GAGGACGATG AACCTGAGCC CAAGTTTGAG AAGTTCCTGG ATGTCGTCGA TCCAGGGAGG 
AAGGGCTACA TCACCCTGGA GGACTACACC TCATTCCTGA TCGACAAGGA GTCAGAGAAC 
ATCAAGTCCA GCGATGAAAT AGAGAATGGC TTCCAGGCCC TGGCAGAGGG CAAGGCCTAT 
ATTACCAAAG ACGACATGAA GCAGGCCCTA ACCCCAGAAC AAGTGCAGTT CTGTGCATCA 
CATATGCAAG AATACGTGGA CCCGCGAGGC CAAAGACACC TTGCTGGTTA TGACTACGTG 
GGCTTCACCA GTTCCTACTT TGGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677904.2
CDS	290..7219
Translation	METSGPKVLETAEAIQERRREVLTRYQRFKELVAERGQKLEESYHYQVFRRDADDLEKWVLEKLKIADDKSYEDLTNIQGKYRKHESLEAEIQAKSRVIPELEEIRETRFAEGHFAHEDTKAHLEQLRTLWDLLLELTRERGALLLRALRLQQYLQECADTLEWIGDKEAIVTSAELGEDWERTEVLHKKFEEFQADLETQKRKLDGMNQYANQCAEEKHPDLPLIKLKQDEVNTAWKRLLSLAGQRQETLANAADLQRFKRDVTEAVQWIKEKEPQLTSEDYGKDLISSEALFHSHKGLERNLAVMQDKVKELSAKADRLMLSHPSDASQIQQMKEELVSNWEHIRALAASRYEKLQASYWYQRFLSDYEELSGWMKEKSALINADELPTDVAGGEALLDRHGQHKHEIDSYDDRFQSAEETGRELLDVGHEASDEVLEKMTILANDWAALRGLWDQRKHQYEQCLYLHLFYRDSEQVDSWMSRQEAFLEDEDLGNSLGSVEALLQKHDDFEEAFNAQEEKITTLDNNATKLIDNDHYDSEKIAALRDSLLSRRDALRARAAMRRRLLEDSWLLQKLYEDSDDLKNWVNKKKKLADDEDYKDTQNLKSRVKKQEDFKKELQDNQRLLNTLEKTGQEMIEANHYAADKVTARVAEVVSLWKELLAAMEQKGTQLHDANQELQFKNNAEDLEHWLDKAEEQAASEDYGKGLADIQSLLRKHGLLESAVATRQDQVDSLTDRAAYFEEIGHPNAGDIQARRESLVSRFEALKEPMATRKKKLIDFLHLLQLFRDTEDEEAWIEETEPSVASTYLGKDLIASKKLLNRHQVIQDDIANHEPHVRMITERGNKMVEEGHFAAEDVASKVKALNDNMESLQARAARRQNDLEANVQFQQYLADLHEAEAWIQEKAPIVDNTNYGADEEAAGALLKKHEAFLVDLSAFGNSMQALRDQAEACQQQQAAPVEGAAREQRVVALYDFEARSNREVTMKKDDVLTLLSSINKDWWKVEAGDHQGFVPAVYVRKLAHDEFPTLPQRRREEPGSITERQEQIEKQYRSLLDRAEERRCRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQTDLKANEPRLRDINKVADDLLFEKLLTPEGAQLRQELNNRWDSLQRLAEEQRQLLGSAHAVQMFHREADDTKEQIEKKLQALRAADPGSDLFSVQALQRQHEVFERDLMPLEEKVTMLRETAERLSESHPDAAGDLQNQGMELKETWDDLLGCTEDRKHDLNEARQFYTFLNRARDLENWMSGIGGMVSSEELAEDLTGTEILLERHSGHYADMEAEAPTFQALEEFGGELVRSGHRDSPEIQEKLEAVRQEKGDLEKAWDQRKKMLHQCLELQLFHGSCDQAESWMMARENFLRSERAGPLDSLEALMKKRDDLEKAINAQDKKITELGNVAERLIADDHYAKEEIAARLQRVLDRWKALKKLLVAEQTKLGDYADVKQFYRDLEDLEEWIGEMLPTACDESYKDPTNIQRKYLKHKAFENEVKGRTEQVKGVLTLGNSLIERQACDGNEETVRKQLQELQRQWDYLLEKTADKGEKLDEASRQQRFNTSIQDFKFWLSEAETLLAMKDQARDLASAGNLLKKHQLLETEMSAREDALKDLNESATHLLSSGTFNVDQIVEQRDNVNERFLNVRNLAAAHHETLKEAYALFQFFQDLDDEESWIEEKLLRVRSQDYGRDLQGVQNLLRKHKRLAGELQAHEPTIQNILDAAERLGDKAAVGREEIQERLTHLFQQWEKLKELASTRGLRLEESLEYLKFMENAEEEEAWISEKEAMVARGGSGDTLAATQSLLKKHKALENDFAVHETRVQDVCAQGEDILNKEKSQHTEKVATKIQTLTEKIPSLAKAMAVWKLQLEDDYDFQQFNWKADVVEAWIAEKEASLKTNGNDTDLTASLALLAKQDTLDASLQSFQQDRLSEITDLKDQLVAAQHSQTKAIEERHAALLRRWGELLEASAAHRQNLLEKQLPLKKEREQELQKEEERQVKNFEMCQEFEQNASAFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIEDLGDNLEEALVLDIKYSTIGLAQQWDQLHQLGTRMQHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLSHKDFRSCLRGLNYYLPMVEDDEPEPKFEKFLDVVDPGRKGYITLEDYTSFLIDKESENIKSSDEIENGFQALAEGKAYITKDDMKQALTPEQVQFCASHMQEYVDPRGQRHLAGYDYVGFTSSYFGN

Target ORF information:

RefSeq Version	XM_019822345.2
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019822345.2

ORF Insert Sequence:

ATGGAGACTA GTGGCCCAAA GGTGCTGGAA ACTGCGGAGG CGATCCAGGA GAGACGTCGG 
GAGGTGCTGA CTCGGTACCA GAGGTTCAAG GAGCTGGTTG CGGAGAGGGG TCAGAAGCTC 
GAAGAATCCT ATCATTACCA GGTTTTCAGA CGGGATGCAG ATGACCTAGA GAAGTGGGTC 
TTAGAGAAAC TCAAGATTGC AGATGATAAG AGCTACGAAG ATCTAACTAA CATACAGGGC 
AAGTATCGGA AGCATGAATC CTTGGAAGCG GAAATACAAG CAAAGTCCAG GGTCATTCCT 
GAACTGGAAG AGATCAGGGA AACCCGGTTT GCCGAGGGGC ATTTTGCCCA TGAGGACACC 
AAGGCCCATC TGGAACAGCT GCGCACGCTG TGGGACCTCC TTCTGGAGCT GACCCGGGAG 
AGGGGCGCCC TGTTGCTGCG GGCCCTGCGT CTACAGCAGT ACCTGCAGGA GTGCGCGGAC 
ACCTTGGAGT GGATCGGAGA CAAGGAGGCT ATAGTGACGT CAGCAGAGCT GGGTGAGGAC 
TGGGAACGCA CAGAGGTCCT GCATAAGAAG TTTGAGGAGT TCCAGGCGGA CCTGGAAACT 
CAGAAAAGAA AATTGGATGG AATGAACCAG TATGCCAACC AGTGTGCCGA GGAGAAACAT 
CCTGACCTGC CCTTGATTAA GCTAAAGCAG GATGAGGTGA ACACCGCCTG GAAGCGTCTC 
CTCAGCCTGG CTGGGCAGCG ACAGGAGACA CTGGCCAATG CTGCAGACCT CCAGCGATTC 
AAAAGGGATG TGACGGAGGC CGTCCAGTGG ATCAAAGAGA AGGAGCCTCA ACTCACCTCT 
GAGGACTATG GCAAAGATCT CATTAGCTCT GAGGCGCTGT TCCACAGTCA CAAAGGTCTT 
GAGAGGAACC TTGCAGTCAT GCAGGACAAG GTAAAGGAGT TGAGTGCCAA AGCAGACAGG 
CTGATGCTTT CCCACCCTTC AGATGCATCT CAGATCCAAC AAATGAAGGA GGAGCTGGTC 
TCCAACTGGG AGCACATCCG CGCCTTGGCG GCCAGCAGAT ATGAGAAGCT GCAGGCTTCT 
TATTGGTATC AGCGCTTCTT ATCTGACTAC GAGGAGCTCT CAGGGTGGAT GAAAGAGAAG 
TCTGCTCTGA TCAATGCTGA TGAGCTGCCC ACAGATGTGG CTGGTGGGGA GGCCCTGCTG 
GACAGGCATG GGCAGCACAA GCACGAGATT GACTCTTATG ATGACCGATT CCAATCTGCT 
GAGGAGACTG GTCGGGAACT GCTGGACGTC GGTCATGAAG CCTCTGATGA AGTTCTGGAA 
AAGATGACCA TACTCGCCAA CGACTGGGCT GCCCTGCGAG GGCTGTGGGA CCAGCGGAAG 
CATCAATACG AGCAGTGCTT GTACTTGCAC CTCTTCTATC GTGACAGTGA GCAGGTGGAC 
AGCTGGATGA GCAGACAGGA GGCCTTCCTG GAAGATGAGG ACCTGGGGAA CTCCCTGGGC 
AGCGTGGAAG CCCTTCTGCA GAAGCATGAT GACTTTGAGG AGGCCTTCAA TGCCCAGGAG 
GAGAAGATCA CAACCTTAGA CAACAATGCC ACCAAGCTGA TCGATAACGA CCATTATGAC 
TCCGAGAAGA TCGCTGCTCT CCGGGATAGC CTCTTGTCCC GGCGGGATGC CCTACGTGCC 
CGGGCTGCCA TGCGGCGCAG ACTTCTGGAG GATTCGTGGC TTCTGCAGAA ACTGTATGAG 
GACTCAGATG ACCTAAAGAA CTGGGTCAAC AAGAAGAAAA AGTTGGCCGA TGATGAAGAC 
TACAAGGATA CTCAGAACTT GAAGAGCCGG GTTAAAAAGC AAGAAGACTT CAAAAAGGAG 
TTGCAAGATA ATCAACGCCT GCTCAACACG CTGGAGAAAA CTGGCCAGGA AATGATTGAG 
GCTAATCACT ACGCCGCGGA CAAGGTGACT GCTCGTGTGG CTGAGGTTGT CAGTCTCTGG 
AAGGAGCTGC TGGCAGCCAT GGAGCAGAAA GGGACGCAGT TGCATGACGC TAACCAGGAA 
CTACAGTTCA AGAACAACGC GGAGGACCTA GAACACTGGC TGGACAAGGC AGAAGAGCAG 
GCTGCCTCCG AGGACTACGG GAAAGGCCTG GCAGACATCC AAAGCCTGCT CCGGAAACAT 
GGCCTCCTGG AGTCCGCTGT GGCCACCCGC CAGGATCAGG TGGACAGCCT CACAGACCGG 
GCAGCATATT TTGAAGAAAT AGGCCATCCC AATGCTGGTG ACATACAAGC AAGGCGGGAG 
TCCCTGGTGT CCCGGTTTGA AGCCCTGAAG GAGCCAATGG CCACCCGGAA AAAGAAGCTC 
ATTGACTTTC TGCACCTGCT GCAGCTGTTC AGGGACACAG AGGATGAGGA AGCCTGGATA 
GAAGAGACAG AGCCTTCGGT GGCTTCCACC TACCTTGGCA AGGACCTGAT TGCCTCCAAG 
AAACTTCTGA ACAGACACCA GGTCATCCAG GATGACATCG CCAATCATGA GCCACACGTC 
CGAATGATAA CGGAGAGGGG AAACAAAATG GTAGAGGAAG GGCACTTTGC TGCAGAAGAT 
GTGGCCTCCA AGGTCAAGGC TTTAAATGAC AACATGGAGT CTCTCCAGGC TCGAGCAGCT 
AGACGGCAGA ACGATCTTGA GGCCAATGTC CAGTTCCAGC AGTACCTGGC TGACCTGCAT 
GAAGCCGAAG CGTGGATCCA AGAGAAGGCG CCTATTGTAG ACAACACTAA CTATGGGGCC 
GATGAAGAAG CAGCTGGGGC TCTTCTAAAG AAGCACGAGG CCTTCCTGGT GGACCTGAGT 
GCCTTTGGGA ACAGTATGCA AGCCCTGAGG GACCAGGCGG AGGCCTGCCA GCAACAACAG 
GCTGCACCAG TGGAGGGAGC TGCCCGAGAA CAGAGGGTGG TGGCTTTGTA TGACTTCGAG 
GCCCGTAGCA ATCGAGAAGT CACCATGAAG AAAGATGACG TCTTAACTCT GCTCAGTTCC 
ATCAACAAGG ACTGGTGGAA GGTGGAAGCT GGTGACCACC AAGGCTTTGT GCCCGCTGTC 
TACGTCAGGA AACTGGCTCA CGATGAGTTT CCCACACTCC CGCAGCGGCG ACGAGAAGAA 
CCAGGCAGCA TCACTGAGCG CCAGGAACAG ATTGAGAAGC AGTACCGCTC CCTCCTCGAT 
AGGGCCGAGG AACGCAGATG TCGCCTGCTG CAACGCTACA ACGAGTTCTT GCTGGCCTAT 
GAGGCGGGCG ACATGCTGGA GTGGATCCAA GAGAAAAAGG CAGAGAACAC CGGGGTGGAA 
CTGGATGATG TCTGGGAGCT GCAGAAGAAG TTTGACGAGT TCCAAACGGA TCTGAAGGCC 
AATGAGCCTC GGCTGAGGGA TATCAACAAG GTGGCGGATG ATCTTCTGTT TGAAAAGCTT 
CTAACGCCAG AAGGAGCTCA GCTACGTCAG GAGTTGAATA ATCGCTGGGA CTCCTTGCAG 
AGGCTTGCAG AAGAGCAGCG ACAGCTGCTG GGCAGCGCCC ATGCCGTCCA GATGTTTCAC 
AGAGAAGCAG ATGACACGAA GGAGCAGATT GAGAAGAAGC TCCAGGCCCT CAGGGCGGCT 
GATCCCGGCT CAGACCTGTT CAGCGTTCAG GCCCTTCAGC GGCAGCATGA GGTCTTTGAG 
AGAGACCTTA TGCCTCTGGA AGAGAAGGTG ACCATGTTGA GGGAGACGGC AGAGCGGCTC 
AGTGAGTCCC ACCCAGATGC CGCTGGTGAT CTGCAGAACC AGGGAATGGA GCTGAAGGAG 
ACATGGGATG ACCTTCTAGG GTGCACGGAG GACCGTAAGC ATGACTTAAA TGAGGCCCGG 
CAATTCTACA CGTTCCTCAA CAGAGCCAGG GACCTAGAGA ACTGGATGAG TGGCATTGGT 
GGCATGGTAT CATCAGAGGA GCTGGCTGAG GATTTAACTG GCACAGAGAT CCTGCTGGAG 
CGACACTCGG GGCATTATGC TGACATGGAG GCCGAGGCTC CCACCTTCCA GGCCTTAGAG 
GAATTCGGCG GAGAACTTGT AAGAAGTGGG CACCGTGACA GCCCTGAAAT TCAAGAAAAG 
CTTGAAGCGG TCAGGCAAGA GAAAGGTGAC TTAGAGAAAG CCTGGGATCA ACGCAAGAAG 
ATGCTGCATC AATGTCTGGA GTTGCAGTTG TTCCATGGGA GCTGTGATCA GGCCGAGAGC 
TGGATGATGG CACGAGAGAA TTTCCTGAGG TCAGAGAGAG CGGGCCCCTT AGACAGTCTG 
GAGGCCTTGA TGAAGAAACG GGATGATTTG GAGAAAGCAA TCAATGCCCA GGACAAGAAG 
ATCACAGAGC TAGGTAATGT TGCGGAGCGG CTCATTGCTG ATGACCACTA TGCCAAGGAG 
GAGATTGCTG CTCGGTTACA ACGGGTGCTG GACAGATGGA AGGCTCTCAA GAAACTACTG 
GTTGCTGAGC AGACAAAACT TGGTGACTAT GCTGACGTCA AACAATTCTA CCGTGACCTC 
GAGGATCTGG AGGAATGGAT CGGTGAGATG CTGCCCACGG CCTGTGATGA ATCCTACAAA 
GATCCCACCA ACATTCAGAG GAAATACCTG AAGCACAAGG CCTTTGAAAA TGAGGTCAAA 
GGCCGAACCG AGCAGGTGAA GGGAGTCCTC ACCTTGGGGA ACTCTTTGAT TGAGCGGCAG 
GCATGTGATG GCAATGAAGA AACCGTGAGG AAACAACTGC AGGAACTGCA GAGACAGTGG 
GACTACCTGC TTGAGAAAAC AGCTGACAAA GGAGAAAAGC TCGATGAGGC GAGTCGCCAA 
CAGAGGTTCA ACACAAGCAT CCAGGACTTT AAGTTCTGGC TCTCAGAGGC AGAGACACTG 
CTGGCCATGA AAGATCAGGC CAGGGATTTG GCTTCGGCCG GAAACTTGCT CAAGAAACAT 
CAGCTGTTGG AAACGGAGAT GTCGGCCCGA GAGGACGCTC TCAAGGACCT GAATGAATCA 
GCAACTCATC TGCTCTCCAG TGGGACTTTC AACGTTGACC AGATTGTGGA GCAAAGGGAT 
AATGTCAATG AACGCTTCCT GAATGTCCGG AACTTAGCGG CTGCACACCA CGAGACGCTG 
AAAGAGGCCT ATGCCTTGTT CCAGTTCTTC CAGGATCTAG ACGATGAGGA ATCGTGGATA 
GAGGAGAAGC TGTTACGAGT GAGGTCCCAG GACTACGGCA GGGACCTGCA GGGCGTTCAG 
AACCTGCTGA GGAAGCACAA GCGCCTGGCA GGGGAGCTGC AGGCACACGA ACCCACCATC 
CAGAACATTC TGGATGCGGC AGAGAGGCTG GGAGACAAGG CTGCTGTGGG GCGAGAGGAG 
ATCCAGGAGC GCCTGACTCA TCTTTTTCAA CAATGGGAGA AGCTCAAAGA GTTGGCCAGC 
ACTCGAGGGC TCCGACTGGA AGAATCCCTA GAATACCTGA AGTTCATGGA GAATGCTGAG 
GAGGAGGAAG CTTGGATCAG TGAAAAGGAA GCTATGGTTG CCCGAGGGGG TTCTGGGGAC 
ACACTGGCCG CTACTCAGAG CTTGCTAAAG AAGCACAAAG CATTGGAAAA TGACTTTGCC 
GTCCATGAGA CCCGAGTGCA AGATGTGTGT GCTCAAGGAG AAGATATTTT AAATAAGGAG 
AAAAGTCAGC ACACGGAGAA GGTAGCCACC AAGATACAGA CTCTGACCGA AAAGATCCCT 
TCTCTGGCTA AGGCGATGGC TGTTTGGAAG TTGCAATTGG AAGATGATTA TGACTTTCAG 
CAGTTCAACT GGAAGGCTGA TGTGGTGGAG GCCTGGATAG CTGAGAAGGA AGCAAGTCTG 
AAGACCAATG GCAATGATAC AGACCTCACT GCATCACTTG CTCTCCTGGC AAAGCAGGAC 
ACGCTGGATG CCAGCCTACA GAGCTTCCAG CAAGACAGGC TGTCGGAGAT AACTGACCTG 
AAGGACCAGC TGGTGGCCGC TCAGCACAGC CAGACCAAAG CCATTGAGGA GCGCCATGCT 
GCCCTGCTGA GGCGCTGGGG GGAGCTGCTG GAAGCCTCTG CAGCCCACAG ACAGAACTTG 
CTGGAGAAGC AGCTTCCCCT AAAGAAGGAA CGGGAGCAGG AGCTACAAAA AGAGGAGGAG 
AGACAGGTCA AGAACTTTGA GATGTGCCAG GAGTTTGAGC AGAATGCCAG TGCCTTCCTT 
CAGTGGATCT TGGAGACCAG GGCTTATTTT CTGGATGGGT CTTTGCTCAA AGAGACGGGA 
ACCCTGGAAT CCCAGTTGGA AGCAAACAAA CGGAAACAGA AGGAGATTCA GGCCATGAAG 
CGCCAGCTGA CCAAGATTGA GGATCTGGGA GACAACTTAG AAGAGGCTCT GGTCCTGGAC 
ATCAAGTACA GCACCATCGG GCTGGCCCAG CAGTGGGACC AGCTCCACCA GCTGGGAACA 
CGGATGCAGC ACAACCTGGA ACAACAGATC CAAGCCAAAG ACACCATAGG TGTGAGTGAG 
GAGACATTGA AGGAGTTCAG CACAACCTAT AAACATTTTG ATGAGAATCT GACGGGGCGC 
TTGAGTCACA AAGATTTCCG GTCCTGCCTC AGAGGACTCA ACTACTACCT GCCCATGGTG 
GAGGACGATG AACCTGAGCC CAAGTTTGAG AAGTTCCTGG ATGTCGTCGA TCCAGGGAGG 
AAGGGCTACA TCACCCTGGA GGACTACACC TCATTCCTGA TCGACAAGGA GTCAGAGAAC 
ATCAAGTCCA GCGATGAAAT AGAGAATGGC TTCCAGGCCC TGGCAGAGGG CAAGGCCTAT 
ATTACCAAAG ACGACATGAA GCAGGCCCTA ACCCCAGAAC AAGTGCAGTT CTGTGCATCA 
CATATGCAAG AATACGTGGA CCCGCGAGGC CAAAGACACC TTGCTGGTTA TGACTACGTG 
GGCTTCACCA GTTCCTACTT TGGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTA1 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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