MYO5B cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO5B
Entrez Gene ID	101094380
Full Name	myosin VB
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023241982.1	XP_023097750.1	unconventional myosin-Vb isoform X1
XM_023241983.1	XP_023097751.1	unconventional myosin-Vb isoform X2
XM_019815257.1	XP_019670816.1	unconventional myosin-Vb

Pathways from BioSystems
Gene Ontology

The following MYO5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb52078	XM_019815257.1 Latest version!	Felis catus myosin VB (MYO5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb63289	XM_023241982.1 Latest version!	Felis catus myosin VB (MYO5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb63290	XM_023241983.1 Latest version!	Felis catus myosin VB (MYO5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO5B ( XM_019815257.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019670816.1 Felis catus myosin VB (MYO5B), mRNA.

Price & Availability↑

CloneID	OFb52078
Clone ID Related Accession (Same CDS sequence)	XM_019815257.1
Accession Version	XM_019815257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	unconventional myosin-Vb
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCAA GAGGTGGTAA CTCCCCTGTA TTGTATTATA ACACATGCCA GTGCACGAGG 
GTCTGGATCC CTGACCCTGA TGAAGTGTGG CGCTCAGCTG AGTTAACCAA GGACTACAAG 
GAGGGAGACA CGAGCCTCCA GCTCAGACTG GAAGATGAAA CGATTCGGGA ATACCCTATT 
GATGTCCAAA GCAACCAGCT GCCCTTTTTG CGGAATCCAG ACATCTTGGT GGGAGAAAAT 
GACCTAACTG CCCTTAGCTA TCTACATGAG CCTGCAGTTT TGCATAATTT AAAGGTCCGT 
TTCCTGGAGT CGAACCACAT CTACACTTAC TGTGGTATCG TGCTTGTTGC CATTAATCCT 
TATGAACAGT TGCCAATCTA TGGTCAAGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGGGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAGAAGA ACCAGTCCAT CATAGTCAGC GGGGAGTCTG GAGCCGGCAA GACTGTGTCA 
GCCAAGTACG CCATGCGCTA TTTTGCCACG GTTGGTGGCT CAGCCAGTGA CACCAACATT 
GAAGAGAAGG TCCTGGCATC CAGTCCCATC ATGGAGGCCA TTGGAAATGC TAAAACCACT 
CGCAATGACA ACAGCAGCCG TTTTGGGAAG TACATTCAGA TTGGCTTTGA TAAAAGGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC CTGTTGGAGA AGTCCAGAGT GGTCTTTCAG 
GCAGATGACG AGCGGAATTA CCACATCTTT TACCAGCTCT GTGCTGCCGC CAGTCTCCCG 
GAATTTAAGG AGCTTGCACT GACATGCGCA GAGGACTTCT TCTATACCTC ACAGGGAAGA 
GACACGTGTA TCGAGGGTGT CGATGATGCC GAGGACTTTG AGAAGACGCG GCAAGCCTTC 
ACACTCCTCG GAGTGAGAGA GTCCCATCAG ATAAGCATTT TTAAGATAAT TGCTTCTATC 
CTGCACCTTG GAAATGTGGA GATTCAGGCT GAGCGTGATG GTGATTCCTG TAGTATATCA 
CCTCAGGATG AACACTTGAA CAGCTTCTGC CGACTGCTAG GGGTGGAGCA CAGTCAGATG 
GAGCACTGGC TATGCCATCG AAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACTATG 
TCCCTGCAGC AGGTGGTCAA CGCCCGCAAC GCCCTAGCTA AGCACATCTA TGCCCAGCTG 
TTCGGCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CGCCTCTCAA GCAACACTCC 
TTCATTGGCG TCCTGGACAT CTATGGGTTT GAGACATTTG AGGTGAACAG CTTTGAGCAG 
TTCTGTATCA ACTATGCGAA TGAGAAGCTA CAGCAGCAGT TCAACTCGCA CGTGTTCAAA 
CTAGAGCAAG AAGAATATAT GAAGGAGCAG ATACCTTGGA CCTTGATTGA TTTTTACGAT 
AACCAACCTT GTATTGACCT CATAGAAGCT AAACTGGGCA TCTTGGACCT GTTGGACGAA 
GAGTGTAAGG TCCCTAAAGG AACTGACCAG AACTGGGCAC AGAAGCTCTA TGACCGGCAC 
TCTGGCAGCC AGCACTTCCA GAAACCCCGC ATGTCCAACA CAGCCTTCAT TGTGGTCCAC 
TTTGCTGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTGG AGAAGAACAG AGACACGGTG 
TATGAAGAGC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CACTAGTGGC TGATTTGTTT 
CATGATGACA AAGACTCTGT TCCTGCCACC ACTGCATCTG GGAAGGGCTC ATCCTCAAAG 
ATCAACATTC GCTCTGCCAG ACCCCCTCTG AAAGTCTCCA ACAAGGAACA CAAGAAAACC 
GTGGGCCACC AGTTCCGTAC CTCCCTGCAT CTGCTCATGG AGACCCTGAA TGCCACGACC 
CCACACTACG TGCGCTGCAT CAAGCCAAAT GACGAGAAGC TCCCCTTCCA CTTTGACCCA 
AAGAGAGCAG TGCAGCAGCT CAGAGCGTGT GGGGTGTTGG AGACCATCCG AATCAGTGCA 
GCCGGCTACC CATCCAGGTG GGCCTACCAC GACTTCTTCA ACCGGTATCG GGTGCTGGTC 
AAGAAGAGAG AGCTCGCCAA CACAGACAAA AAGGCCATCT GCAGATCTGT CCTGGAAAGC 
CTCATCAAGG ATCCCGACAA GTTCCAGTTT GGCCGCACCA AGATCTTTTT CCGGGCAGGC 
CAGGTGGCTT ACCTGGAGAA GCTTCGGGCT GACAAGTTCC GGGCAGCCAC GATCATGATC 
CAGAAAACTG TCCGGGGCTG GCTGCAGAAG GTGAAATACC GCAGGCTGAA GGCCACTACC 
TTAACTCTGC AGCGGTATTG CCGAGGGCTC CTGGCCCGAA GGCTGGCCGA GCATCTGCGG 
AGGACGCGAG CAGCCATCAT GCTCCAGAAG CAGTACCGCA TGCGGAGGGC CCGCCTGGCC 
TACCAGAGGG TCCACAGGGC CACCGTCACC ATCCAGGCCT TCACTCGGGG CATGTTCGTG 
CGGAGAATCT ACCAGCAGGT CCTCATGGAG CACAAGGCCA CCATCCTCCA GAAGCACGTG 
CGGGGCTGGG TGGCACGTAG GCGCTTTCAG CGGCTGCGGG GCGCGGCCAT CGTCATCCAG 
TGTGCCTTCC GCATGCTTAA GGCCAAGCAG GAGCTGAAGG CCCTCAAGAT CGAGGCCCGC 
TCAGCAGAGC ACCTGAAACG TCTCAACGTG GGCATGGAGA ACAAGGTGGT CCAGCTGCAG 
CGGAAGATTG ACGACCAGAA CAAAGAGTTC AAGACTCTGT CGGAGCAGTT GTCGGCGGTC 
ACCTCCACAC ACACCATGGA GGTAGAGAAG TTGAAGAAGG AGCTGGCCCG CTACCAGCAG 
AGCCAAGGTG AGGACGGCAG CCTGAGGCTG CAGGAGGAGG TGGAGAGCCT GCGGGCGGAG 
CTGCAGAGGG CCCACTCGGA GCGCAAGATC CTGGAGGACG CCCACACCAA GGAGAAGGAC 
GAGCTGAGAA AGCGCGTTGC AGACCTGGAG GAAGAAAATG CTCTCTTAAA AGATGAGAAA 
GAACAGCTTA ACAACCAAAT CCTGTGCCAA GCAAAAGACG AATCTGCCCA AAACTCTGCC 
AAGGAAAATC TGATGAAGAG GGAGCTGGAG GAGGAGCGCT CCCGGTACCA GAACCTCGTG 
AAGGAATATT CACGGTTGGA GCAGAGATAC GACAACCTTC GGGATGAGAT GACCATCATC 
AAGCAAACCC CAGGGCACAG GCGGAACCCA TCAAACCAGA GCAGTTTAGA ATCTGACTCC 
AATTACCCCT CCATCTCCAC ATCTGAGGTC GGAGACACAG AGGATGCCNN NNTTCCCCTG 
AAGGACATCG GCCTGGAAAA GGCAGCCATG GACATGACCG TCTTCCTGAA ACTGCAGAAG 
AGAGTGCGCG AGCTGGAGCA GGAGAGGAAG AAACTGCAGG TGCAGCTGGA GAAGAGGGAG 
CAGCAGGACA GCCGGAAGGT CCAGGTGGAA CAAGCAAAGA ATGACCTGGA TTTGGACCAG 
GATGCAGATC TAGCCTACAA CAGTCTGAAG AGGCAAGAGC TAGAGTCGGA GAACAAGAAG 
CTGAAGAACG ACCTGAACGA GCTGAGGAAA GCCGTGGCTG ACCAAGCCAC ACAGAACAAC 
TCCAGCCACA GCTCCCCCGA CAGCTACAGC CTGCTGCTGA ACCAGCTCAA GCTGGCCAAC 
GAGGAACTTG AGGTTCGCAA GGAGGAGGTG CTCATCCTGA GGACGCAGAT CGTCAGCGCC 
GACCAGCGCC GACTCGCCGG CAAGAGCACG GAGCCGAACA TTAATGCCAG AACAAGTTGG 
CCTAACAGTG AGAAGCATGT AGATCAGGAA GATGCCATCG AGGCCTATCA TGGGGTCTGC 
CAGACAAACA GCAAGACTGA GGATTGGGGA TATTTGAATG AAGATGGAGA GCTCGGCTTG 
GCCTACCAAG GCCTAAAGCA AGTTGCCAGG TTGCTGGAGG CCCAGCTGCA GGCCCAGAGC 
CGCGAGCATG AGGAGGAGGT GGAGGGGCTC CGGGCCCAGG TGGAAGCCCT GAAGGAGGAA 
CTGGACAAGC AGCAGCAGAC CTTCTGCCAG ACGCTGCTGC TCTCCCCAGA GGCCCAGGTG 
GAATTTGGCG TCCAGCAGGA GATATCCCGG CTCACCAATG AGAACCTGGA CCTTAAAGAA 
CTGGTAGAAA AGCTGGAAAA GAATGAGAGG AAGCTCAAGA AGCAGCTGAA GATTTACATG 
AAGAAAGTCC AGGACCTAGA AGCTGCCCAG GCGCTGGCCC AGAGTGAGAG GAGACGCCAT 
GAACTCAACA GGCAGGTCAC TGTGCAGCGG AAGGAGAAGG ACTTCCAGGG CATGCTGGAG 
TACCACAAGG AAGATGAGGC CCTTCTCATT CGGAACCTGG TGACAGACCT GAAGCCGCAG 
GCGCTGGCGG GCACGGTGCC CTGTCTTCCT GCCTACATTC TCTACATGTG CATCAGGCAC 
GCAGATTACG TCAACGACGA CCTCAAGGTG CACTCCTTGC TGACCTCCAC CATCAACGGC 
ATTAAGAAAG TCCTGAAGAA GCACAATGAT GACTTCGAGA TGACATCATT CTGGTTATCC 
AATACCTGCC GCCTCCTTCA TTGTTTGAAG CAGTATAGCG GGGATGAGGG CTTCATGACT 
CAGAATACTG CCAAACAGAA TGAGCACTGT CTTAAGAACT TTGACCTCAC TGAATACCGC 
CAGGTGCTGA GTGACCTTTC CATTCAAATC TACCAGCAAC TCATTAAAAT CGCCGAGGGT 
GTGTTGCAGC CTATGATAGT TTCTGCCATG TTGGAAAATG AGAGCATTCA GGGTCTATCT 
GGTGTGAAGC CAACAGGCTA CCGCAAGCGC TCCTCCAGCA TGGCGGACGG GGACAACTCC 
TACTGCCTGG AAGCCGTCAT CCGCCAGATG AATTCCTTTC ACACAGTCAT GTGTGACCAG 
GGCCTGGACC CGGAGATCAT CCTGCAGGTG TTCAAGCAGC TCTTCTACAT GATCAACGCT 
GTGACTCTCA ACAACCTGCT CCTGCGGAAG GACGTCTGCT CTTGGAGCAC AGGCATGCAG 
CTCAGGTACA ATATAAGTCA ACTTGAAGAG TGGCTTCGGG GAAGAAACCT TCACCAGAGC 
GGAGCAGTCC AGACCATGGA ACCTCTGATC CAAGCAGCCC AGCTCCTACA GTTAAAGAAG 
AAAACCCCTG AGGACGCAGA GGCCATCTGC TCGCTGTGCA CCTCCCTCAG CACCCAGCAG 
ATTGTCAAAA TTTTAAACCT TTATACTCCC CTGAATGAAT TTGAAGAACG GGTAACCGTG 
GCCTTTATAC GAACAATCCA GGCACAACTA CAAGAGCGGA ATGACCCTCA GCAACTGCTG 
TTAGACTTCA AGCACATGTT TCCAGTGTTG TTTCCATTTA ACCCATCCTC TCTGACCATG 
GACTCCATCC ACATCCCAGC ATATCTCAAT CTGGAGTTCC TCAATGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019670816.1
CDS	16..5589
Translation	MDPRGGNSPVLYYNTCQCTRVWIPDPDEVWRSAELTKDYKEGDTSLQLRLEDETIREYPIDVQSNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGRDTCIEGVDDAEDFEKTRQAFTLLGVRESHQISIFKIIASILHLGNVEIQAERDGDSCSISPQDEHLNSFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARNALAKHIYAQLFGWIVEHINKALHTPLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHSGSQHFQKPRMSNTAFIVVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSVPATTASGKGSSSKINIRSARPPLKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKKRELANTDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQKVKYRRLKATTLTLQRYCRGLLARRLAEHLRRTRAAIMLQKQYRMRRARLAYQRVHRATVTIQAFTRGMFVRRIYQQVLMEHKATILQKHVRGWVARRRFQRLRGAAIVIQCAFRMLKAKQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSTHTMEVEKLKKELARYQQSQGEDGSLRLQEEVESLRAELQRAHSERKILEDAHTKEKDELRKRVADLEEENALLKDEKEQLNNQILCQAKDESAQNSAKENLMKRELEEERSRYQNLVKEYSRLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDAnullnullPLKDIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKREQQDSRKVQVEQAKNDLDLDQDADLAYNSLKRQELESENKKLKNDLNELRKAVADQATQNNSSHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIVSADQRRLAGKSTEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSREHEEEVEGLRAQVEALKEELDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERRRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQALAGTVPCLPAYILYMCIRHADYVNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAVIRQMNSFHTVMCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVQTMEPLIQAAQLLQLKKKTPEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQERNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPAYLNLEFLNEV

Target ORF information:

RefSeq Version	XM_019815257.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus myosin VB (MYO5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019815257.1

ORF Insert Sequence:

ATGGATCCAA GAGGTGGTAA CTCCCCTGTA TTGTATTATA ACACATGCCA GTGCACGAGG 
GTCTGGATCC CTGACCCTGA TGAAGTGTGG CGCTCAGCTG AGTTAACCAA GGACTACAAG 
GAGGGAGACA CGAGCCTCCA GCTCAGACTG GAAGATGAAA CGATTCGGGA ATACCCTATT 
GATGTCCAAA GCAACCAGCT GCCCTTTTTG CGGAATCCAG ACATCTTGGT GGGAGAAAAT 
GACCTAACTG CCCTTAGCTA TCTACATGAG CCTGCAGTTT TGCATAATTT AAAGGTCCGT 
TTCCTGGAGT CGAACCACAT CTACACTTAC TGTGGTATCG TGCTTGTTGC CATTAATCCT 
TATGAACAGT TGCCAATCTA TGGTCAAGAT GTCATCTATG CCTACAGTGG CCAAAACATG 
GGGGACATGG ACCCCCACAT CTTTGCTGTG GCAGAAGAAG CCTACAAGCA GATGGCCAGA 
GATGAGAAGA ACCAGTCCAT CATAGTCAGC GGGGAGTCTG GAGCCGGCAA GACTGTGTCA 
GCCAAGTACG CCATGCGCTA TTTTGCCACG GTTGGTGGCT CAGCCAGTGA CACCAACATT 
GAAGAGAAGG TCCTGGCATC CAGTCCCATC ATGGAGGCCA TTGGAAATGC TAAAACCACT 
CGCAATGACA ACAGCAGCCG TTTTGGGAAG TACATTCAGA TTGGCTTTGA TAAAAGGTAC 
CACATCATTG GGGCCAACAT GAGGACCTAC CTGTTGGAGA AGTCCAGAGT GGTCTTTCAG 
GCAGATGACG AGCGGAATTA CCACATCTTT TACCAGCTCT GTGCTGCCGC CAGTCTCCCG 
GAATTTAAGG AGCTTGCACT GACATGCGCA GAGGACTTCT TCTATACCTC ACAGGGAAGA 
GACACGTGTA TCGAGGGTGT CGATGATGCC GAGGACTTTG AGAAGACGCG GCAAGCCTTC 
ACACTCCTCG GAGTGAGAGA GTCCCATCAG ATAAGCATTT TTAAGATAAT TGCTTCTATC 
CTGCACCTTG GAAATGTGGA GATTCAGGCT GAGCGTGATG GTGATTCCTG TAGTATATCA 
CCTCAGGATG AACACTTGAA CAGCTTCTGC CGACTGCTAG GGGTGGAGCA CAGTCAGATG 
GAGCACTGGC TATGCCATCG AAAGCTGGTC ACCACCTCAG AGACCTACGT CAAGACTATG 
TCCCTGCAGC AGGTGGTCAA CGCCCGCAAC GCCCTAGCTA AGCACATCTA TGCCCAGCTG 
TTCGGCTGGA TTGTGGAGCA CATCAACAAA GCCCTGCACA CGCCTCTCAA GCAACACTCC 
TTCATTGGCG TCCTGGACAT CTATGGGTTT GAGACATTTG AGGTGAACAG CTTTGAGCAG 
TTCTGTATCA ACTATGCGAA TGAGAAGCTA CAGCAGCAGT TCAACTCGCA CGTGTTCAAA 
CTAGAGCAAG AAGAATATAT GAAGGAGCAG ATACCTTGGA CCTTGATTGA TTTTTACGAT 
AACCAACCTT GTATTGACCT CATAGAAGCT AAACTGGGCA TCTTGGACCT GTTGGACGAA 
GAGTGTAAGG TCCCTAAAGG AACTGACCAG AACTGGGCAC AGAAGCTCTA TGACCGGCAC 
TCTGGCAGCC AGCACTTCCA GAAACCCCGC ATGTCCAACA CAGCCTTCAT TGTGGTCCAC 
TTTGCTGATA AGGTGGAGTA CCTTTCAGAT GGTTTTCTGG AGAAGAACAG AGACACGGTG 
TATGAAGAGC AGATCAACAT CCTGAAGGCC AGCAAGTTCC CACTAGTGGC TGATTTGTTT 
CATGATGACA AAGACTCTGT TCCTGCCACC ACTGCATCTG GGAAGGGCTC ATCCTCAAAG 
ATCAACATTC GCTCTGCCAG ACCCCCTCTG AAAGTCTCCA ACAAGGAACA CAAGAAAACC 
GTGGGCCACC AGTTCCGTAC CTCCCTGCAT CTGCTCATGG AGACCCTGAA TGCCACGACC 
CCACACTACG TGCGCTGCAT CAAGCCAAAT GACGAGAAGC TCCCCTTCCA CTTTGACCCA 
AAGAGAGCAG TGCAGCAGCT CAGAGCGTGT GGGGTGTTGG AGACCATCCG AATCAGTGCA 
GCCGGCTACC CATCCAGGTG GGCCTACCAC GACTTCTTCA ACCGGTATCG GGTGCTGGTC 
AAGAAGAGAG AGCTCGCCAA CACAGACAAA AAGGCCATCT GCAGATCTGT CCTGGAAAGC 
CTCATCAAGG ATCCCGACAA GTTCCAGTTT GGCCGCACCA AGATCTTTTT CCGGGCAGGC 
CAGGTGGCTT ACCTGGAGAA GCTTCGGGCT GACAAGTTCC GGGCAGCCAC GATCATGATC 
CAGAAAACTG TCCGGGGCTG GCTGCAGAAG GTGAAATACC GCAGGCTGAA GGCCACTACC 
TTAACTCTGC AGCGGTATTG CCGAGGGCTC CTGGCCCGAA GGCTGGCCGA GCATCTGCGG 
AGGACGCGAG CAGCCATCAT GCTCCAGAAG CAGTACCGCA TGCGGAGGGC CCGCCTGGCC 
TACCAGAGGG TCCACAGGGC CACCGTCACC ATCCAGGCCT TCACTCGGGG CATGTTCGTG 
CGGAGAATCT ACCAGCAGGT CCTCATGGAG CACAAGGCCA CCATCCTCCA GAAGCACGTG 
CGGGGCTGGG TGGCACGTAG GCGCTTTCAG CGGCTGCGGG GCGCGGCCAT CGTCATCCAG 
TGTGCCTTCC GCATGCTTAA GGCCAAGCAG GAGCTGAAGG CCCTCAAGAT CGAGGCCCGC 
TCAGCAGAGC ACCTGAAACG TCTCAACGTG GGCATGGAGA ACAAGGTGGT CCAGCTGCAG 
CGGAAGATTG ACGACCAGAA CAAAGAGTTC AAGACTCTGT CGGAGCAGTT GTCGGCGGTC 
ACCTCCACAC ACACCATGGA GGTAGAGAAG TTGAAGAAGG AGCTGGCCCG CTACCAGCAG 
AGCCAAGGTG AGGACGGCAG CCTGAGGCTG CAGGAGGAGG TGGAGAGCCT GCGGGCGGAG 
CTGCAGAGGG CCCACTCGGA GCGCAAGATC CTGGAGGACG CCCACACCAA GGAGAAGGAC 
GAGCTGAGAA AGCGCGTTGC AGACCTGGAG GAAGAAAATG CTCTCTTAAA AGATGAGAAA 
GAACAGCTTA ACAACCAAAT CCTGTGCCAA GCAAAAGACG AATCTGCCCA AAACTCTGCC 
AAGGAAAATC TGATGAAGAG GGAGCTGGAG GAGGAGCGCT CCCGGTACCA GAACCTCGTG 
AAGGAATATT CACGGTTGGA GCAGAGATAC GACAACCTTC GGGATGAGAT GACCATCATC 
AAGCAAACCC CAGGGCACAG GCGGAACCCA TCAAACCAGA GCAGTTTAGA ATCTGACTCC 
AATTACCCCT CCATCTCCAC ATCTGAGGTC GGAGACACAG AGGATGCCNN NNTTCCCCTG 
AAGGACATCG GCCTGGAAAA GGCAGCCATG GACATGACCG TCTTCCTGAA ACTGCAGAAG 
AGAGTGCGCG AGCTGGAGCA GGAGAGGAAG AAACTGCAGG TGCAGCTGGA GAAGAGGGAG 
CAGCAGGACA GCCGGAAGGT CCAGGTGGAA CAAGCAAAGA ATGACCTGGA TTTGGACCAG 
GATGCAGATC TAGCCTACAA CAGTCTGAAG AGGCAAGAGC TAGAGTCGGA GAACAAGAAG 
CTGAAGAACG ACCTGAACGA GCTGAGGAAA GCCGTGGCTG ACCAAGCCAC ACAGAACAAC 
TCCAGCCACA GCTCCCCCGA CAGCTACAGC CTGCTGCTGA ACCAGCTCAA GCTGGCCAAC 
GAGGAACTTG AGGTTCGCAA GGAGGAGGTG CTCATCCTGA GGACGCAGAT CGTCAGCGCC 
GACCAGCGCC GACTCGCCGG CAAGAGCACG GAGCCGAACA TTAATGCCAG AACAAGTTGG 
CCTAACAGTG AGAAGCATGT AGATCAGGAA GATGCCATCG AGGCCTATCA TGGGGTCTGC 
CAGACAAACA GCAAGACTGA GGATTGGGGA TATTTGAATG AAGATGGAGA GCTCGGCTTG 
GCCTACCAAG GCCTAAAGCA AGTTGCCAGG TTGCTGGAGG CCCAGCTGCA GGCCCAGAGC 
CGCGAGCATG AGGAGGAGGT GGAGGGGCTC CGGGCCCAGG TGGAAGCCCT GAAGGAGGAA 
CTGGACAAGC AGCAGCAGAC CTTCTGCCAG ACGCTGCTGC TCTCCCCAGA GGCCCAGGTG 
GAATTTGGCG TCCAGCAGGA GATATCCCGG CTCACCAATG AGAACCTGGA CCTTAAAGAA 
CTGGTAGAAA AGCTGGAAAA GAATGAGAGG AAGCTCAAGA AGCAGCTGAA GATTTACATG 
AAGAAAGTCC AGGACCTAGA AGCTGCCCAG GCGCTGGCCC AGAGTGAGAG GAGACGCCAT 
GAACTCAACA GGCAGGTCAC TGTGCAGCGG AAGGAGAAGG ACTTCCAGGG CATGCTGGAG 
TACCACAAGG AAGATGAGGC CCTTCTCATT CGGAACCTGG TGACAGACCT GAAGCCGCAG 
GCGCTGGCGG GCACGGTGCC CTGTCTTCCT GCCTACATTC TCTACATGTG CATCAGGCAC 
GCAGATTACG TCAACGACGA CCTCAAGGTG CACTCCTTGC TGACCTCCAC CATCAACGGC 
ATTAAGAAAG TCCTGAAGAA GCACAATGAT GACTTCGAGA TGACATCATT CTGGTTATCC 
AATACCTGCC GCCTCCTTCA TTGTTTGAAG CAGTATAGCG GGGATGAGGG CTTCATGACT 
CAGAATACTG CCAAACAGAA TGAGCACTGT CTTAAGAACT TTGACCTCAC TGAATACCGC 
CAGGTGCTGA GTGACCTTTC CATTCAAATC TACCAGCAAC TCATTAAAAT CGCCGAGGGT 
GTGTTGCAGC CTATGATAGT TTCTGCCATG TTGGAAAATG AGAGCATTCA GGGTCTATCT 
GGTGTGAAGC CAACAGGCTA CCGCAAGCGC TCCTCCAGCA TGGCGGACGG GGACAACTCC 
TACTGCCTGG AAGCCGTCAT CCGCCAGATG AATTCCTTTC ACACAGTCAT GTGTGACCAG 
GGCCTGGACC CGGAGATCAT CCTGCAGGTG TTCAAGCAGC TCTTCTACAT GATCAACGCT 
GTGACTCTCA ACAACCTGCT CCTGCGGAAG GACGTCTGCT CTTGGAGCAC AGGCATGCAG 
CTCAGGTACA ATATAAGTCA ACTTGAAGAG TGGCTTCGGG GAAGAAACCT TCACCAGAGC 
GGAGCAGTCC AGACCATGGA ACCTCTGATC CAAGCAGCCC AGCTCCTACA GTTAAAGAAG 
AAAACCCCTG AGGACGCAGA GGCCATCTGC TCGCTGTGCA CCTCCCTCAG CACCCAGCAG 
ATTGTCAAAA TTTTAAACCT TTATACTCCC CTGAATGAAT TTGAAGAACG GGTAACCGTG 
GCCTTTATAC GAACAATCCA GGCACAACTA CAAGAGCGGA ATGACCCTCA GCAACTGCTG 
TTAGACTTCA AGCACATGTT TCCAGTGTTG TTTCCATTTA ACCCATCCTC TCTGACCATG 
GACTCCATCC ACATCCCAGC ATATCTCAAT CTGGAGTTCC TCAATGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_023241982.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023097750.1 Felis catus myosin VB (MYO5B), transcript variant X1, mRNA.

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CloneID	OFb63289
Clone ID Related Accession (Same CDS sequence)	XM_023241982.1
Accession Version	XM_023241982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCGAGCTCTA CGGCCAGTGC ACGAGGGTCT GGATCCCTGA CCCTGATGAA 
GTGTGGCGCT CAGCTGAGTT AACCAAGGAC TACAAGGAGG GAGACACGAG CCTCCAGCTC 
AGACTGGAAG ATGAAACGAT TCGGGAATAC CCTATTGATG TCCAAAGCAA CCAGCTGCCC 
TTTTTGCGGA ATCCAGACAT CTTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTA 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGTTTCC TGGAGTCGAA CCACATCTAC 
ACTTACTGTG GTATCGTGCT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGGG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGCGGGG AGTCTGGAGC CGGCAAGACT GTGTCAGCCA AGTACGCCAT GCGCTATTTT 
GCCACGGTTG GTGGCTCAGC CAGTGACACC AACATTGAAG AGAAGGTCCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCTAAA ACCACTCGCA ATGACAACAG CAGCCGTTTT 
GGGAAGTACA TTCAGATTGG CTTTGATAAA AGGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACCTGT TGGAGAAGTC CAGAGTGGTC TTTCAGGCAG ATGACGAGCG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCCGCCAGT CTCCCGGAAT TTAAGGAGCT TGCACTGACA 
TGCGCAGAGG ACTTCTTCTA TACCTCACAG GGAAGAGACA CGTGTATCGA GGGTGTCGAT 
GATGCCGAGG ACTTTGAGAA GACGCGGCAA GCCTTCACAC TCCTCGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCTATCCTGC ACCTTGGAAA TGTGGAGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCTC AGGATGAACA CTTGAACAGC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTATG CCATCGAAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACTATGTCCC TGCAGCAGGT GGTCAACGCC 
CGCAACGCCC TAGCCAAGCA CATCTATGCC CAGCTGTTCG GCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACGCC TCTCAAGCAA CACTCCTTCA TTGGCGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT GAACAGCTTT GAGCAGTTCT GTATCAACTA TGCGAATGAG 
AAGCTACAGC AGCAGTTCAA CTCGCACGTG TTCAAACTAG AGCAAGAAGA ATATATGAAG 
GAGCAGATAC CTTGGACCTT GATTGATTTT TACGATAACC AACCTTGTAT TGACCTCATA 
GAAGCTAAAC TGGGCATCTT GGACCTGTTG GACGAAGAGT GTAAGGTCCC TAAAGGAACT 
GACCAGAACT GGGCACAGAA GCTCTATGAC CGGCACTCTG GCAGCCAGCA CTTCCAGAAA 
CCCCGCATGT CCAACACAGC CTTCATTGTG GTCCACTTTG CTGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA GAACAGAGAC ACGGTGTATG AAGAGCAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAT TTGTTTCATG ATGACAAAGA CTCTGTTCCT 
GCCACCACTG CATCTGGGAA GGGCTCATCC TCAAAGATCA ACATTCGCTC TGCCAGACCC 
CCTCTGAAAG TCTCCAACAA GGAACACAAG AAAACCGTGG GCCACCAGTT CCGTACCTCC 
CTGCATCTGC TCATGGAGAC CCTGAATGCC ACGACCCCAC ACTACGTGCG CTGCATCAAG 
CCAAATGACG AGAAGCTCCC CTTCCACTTT GACCCAAAGA GAGCAGTGCA GCAGCTCAGA 
GCGTGTGGGG TGTTGGAGAC CATCCGAATC AGTGCAGCCG GCTACCCATC CAGGTGGGCC 
TACCACGACT TCTTCAACCG GTATCGGGTG CTGGTCAAGA AGAGAGAGCT CGCCAACACA 
GACAAAAAGG CCATCTGCAG ATCTGTCCTG GAAAGCCTCA TCAAGGATCC CGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTTTTCCGG GCAGGCCAGG TGGCTTACCT GGAGAAGCTT 
CGGGCTGACA AGTTCCGGGC AGCCACGATC ATGATCCAGA AAACTGTCCG GGGCTGGCTG 
CAGAAGGTGA AATACCGCAG GCTGAAGGCC ACTACCTTAA CTCTGCAGCG GTATTGCCGA 
GGGCTCCTGG CCCGAAGGCT GGCCGAGCAT CTGCGGAGGA CGCGAGCAGC CATCATGCTC 
CAGAAGCAGT ACCGCATGCG GAGGGCCCGC CTGGCCTACC AGAGGGTCCA CAGGGCCACC 
GTCACCATCC AGGCCTTCAC TCGGGGCATG TTCGTGCGGA GAATCTACCA GCAGGTCCTC 
ATGGAGCACA AGGCCACCAT CCTCCAGAAG CACGTGCGGG GCTGGGTGGC ACGTAGGCGC 
TTTCAGCGGC TGCGGGGCGC GGCCATCGTC ATCCAGTGTG CCTTCCGCAT GCTTAAGGCC 
AAGCAGGAGC TGAAGGCCCT CAAGATCGAG GCCCGCTCAG CAGAGCACCT GAAACGTCTC 
AACGTGGGCA TGGAGAACAA GGTGGTCCAG CTGCAGCGGA AGATTGACGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCGGA GCAGTTGTCG GCGGTCACCT CCACACACAC CATGGAGGTA 
GAGAAGTTGA AGAAGGAGCT GGCCCGCTAC CAGCAGAGCC AAGGTGAGGA CGGCAGCCTG 
AGGCTGCAGG AGGAGGTGGA GAGCCTGCGG GCGGAGCTGC AGAGGGCCCA CTCGGAGCGC 
AAGATCCTGG AGGACGCCCA CACCAAGGAG AAGGACGAGC TGAGAAAGCG CGTTGCAGAC 
CTGGAGGAAG AAAATGCTCT CTTAAAAGAT GAGAAAGAAC AGCTTAACAA CCAAATCCTG 
TGCCAAGCAA AAGACGAATC TGCCCAAAAC TCTGCCAAGG AAAATCTGAT GAAGAGGGAG 
CTGGAGGAGG AGCGCTCCCG GTACCAGAAC CTCGTGAAGG AATATTCACG GTTGGAGCAG 
AGATACGACA ACCTTCGGGA TGAGATGACC ATCATCAAGC AAACCCCAGG GCACAGGCGG 
AACCCATCAA ACCAGAGCAG TTTAGAATCT GACTCCAATT ACCCCTCCAT CTCCACATCT 
GAGGTCGGAG ACACAGAGGA TGCCCTCCAA CAGGTGGAGG ACATCGGCCT GGAAAAGGCA 
GCCATGGACA TGACCGTCTT CCTGAAACTG CAGAAGAGAG TGCGCGAGCT GGAGCAGGAG 
AGGAAGAAAC TGCAGGTGCA GCTGGAGAAG AGGGAGCAGC AGGACAGCCG GAAGGTCCAG 
GTGGAACAAG CAAAGAATGA CCTGGATTTG GACCAGGATG CAGATCTAGC CTACAACAGT 
CTGAAGAGGC AAGAGCTAGA GTCGGAGAAC AAGAAGCTGA AGAACGACCT GAACGAGCTG 
AGGAAAGCCG TGGCTGACCA AGCCACACAG AACAACTCCA GCCACAGCTC CCCCGACAGC 
TACAGCCTGC TGCTGAACCA GCTCAAGCTG GCCAACGAGG AACTTGAGGT TCGCAAGGAG 
GAGGTGCTCA TCCTGAGGAC GCAGATCGTC AGCGCCGACC AGCGCCGACT CGCCGGCAAG 
AGCACGGAGC CGAACATTAA TGCCAGAACA AGTTGGCCTA ACAGTGAGAA GCATGTAGAT 
CAGGAAGATG CCATCGAGGC CTATCATGGG GTCTGCCAGA CAAACAGCAA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAGCTC GGCTTGGCCT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCGCG AGCATGAGGA GGAGGTGGAG 
GGGCTCCGGG CCCAGGTGGA AGCCCTGAAG GAGGAACTGG ACAAGCAGCA GCAGACCTTC 
TGCCAGACGC TGCTGCTCTC CCCAGAGGCC CAGGTGGAAT TTGGCGTCCA GCAGGAGATA 
TCCCGGCTCA CCAATGAGAA CCTGGACCTT AAAGAACTGG TAGAAAAGCT GGAAAAGAAT 
GAGAGGAAGC TCAAGAAGCA GCTGAAGATT TACATGAAGA AAGTCCAGGA CCTAGAAGCT 
GCCCAGGCGC TGGCCCAGAG TGAGAGGAGA CGCCATGAAC TCAACAGGCA GGTCACTGTG 
CAGCGGAAGG AGAAGGACTT CCAGGGCATG CTGGAGTACC ACAAGGAAGA TGAGGCCCTT 
CTCATTCGGA ACCTGGTGAC AGACCTGAAG CCGCAGGCGC TGGCGGGCAC GGTGCCCTGT 
CTTCCTGCCT ACATTCTCTA CATGTGCATC AGGCACGCAG ATTACGTCAA CGACGACCTC 
AAGGTGCACT CCTTGCTGAC CTCCACCATC AACGGCATTA AGAAAGTCCT GAAGAAGCAC 
AATGATGACT TCGAGATGAC ATCATTCTGG TTATCCAATA CCTGCCGCCT CCTTCATTGT 
TTGAAGCAGT ATAGCGGGGA TGAGGGCTTC ATGACTCAGA ATACTGCCAA ACAGAATGAG 
CACTGTCTTA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTGAGTGA CCTTTCCATT 
CAAATCTACC AGCAACTCAT TAAAATCGCC GAGGGTGTGT TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG CATTCAGGGT CTATCTGGTG TGAAGCCAAC AGGCTACCGC 
AAGCGCTCCT CCAGCATGGC GGACGGGGAC AACTCCTACT GCCTGGAAGC CGTCATCCGC 
CAGATGAATT CCTTTCACAC AGTCATGTGT GACCAGGGCC TGGACCCGGA GATCATCCTG 
CAGGTGTTCA AGCAGCTCTT CTACATGATC AACGCTGTGA CTCTCAACAA CCTGCTCCTG 
CGGAAGGACG TCTGCTCTTG GAGCACAGGC ATGCAGCTCA GGTACAATAT AAGTCAACTT 
GAAGAGTGGC TTCGGGGAAG AAACCTTCAC CAGAGCGGAG CAGTCCAGAC CATGGAACCT 
CTGATCCAAG CAGCCCAGCT CCTACAGTTA AAGAAGAAAA CCCCTGAGGA CGCAGAGGCC 
ATCTGCTCGC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTTTAT 
ACTCCCCTGA ATGAATTTGA AGAACGGGTA ACCGTGGCCT TTATACGAAC AATCCAGGCA 
CAACTACAAG AGCGGAATGA CCCTCAGCAA CTGCTGTTAG ACTTCAAGCA CATGTTTCCA 
GTGTTGTTTC CATTTAACCC ATCCTCTCTG ACCATGGACT CCATCCACAT CCCAGCATAT 
CTCAATCTGG AGTTCCTCAA TGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023097750.1
CDS	404..5953
Translation	MSVSELYGQCTRVWIPDPDEVWRSAELTKDYKEGDTSLQLRLEDETIREYPIDVQSNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGRDTCIEGVDDAEDFEKTRQAFTLLGVRESHQISIFKIIASILHLGNVEIQAERDGDSCSISPQDEHLNSFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARNALAKHIYAQLFGWIVEHINKALHTPLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHSGSQHFQKPRMSNTAFIVVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSVPATTASGKGSSSKINIRSARPPLKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKKRELANTDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQKVKYRRLKATTLTLQRYCRGLLARRLAEHLRRTRAAIMLQKQYRMRRARLAYQRVHRATVTIQAFTRGMFVRRIYQQVLMEHKATILQKHVRGWVARRRFQRLRGAAIVIQCAFRMLKAKQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSTHTMEVEKLKKELARYQQSQGEDGSLRLQEEVESLRAELQRAHSERKILEDAHTKEKDELRKRVADLEEENALLKDEKEQLNNQILCQAKDESAQNSAKENLMKRELEEERSRYQNLVKEYSRLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDALQQVEDIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKREQQDSRKVQVEQAKNDLDLDQDADLAYNSLKRQELESENKKLKNDLNELRKAVADQATQNNSSHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIVSADQRRLAGKSTEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSKTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSREHEEEVEGLRAQVEALKEELDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERRRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQALAGTVPCLPAYILYMCIRHADYVNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAVIRQMNSFHTVMCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVQTMEPLIQAAQLLQLKKKTPEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQERNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPAYLNLEFLNEV

Target ORF information:

RefSeq Version	XM_023241982.1
Organism	Felis catus(domestic cat)
Definition	Felis catus myosin VB (MYO5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023241982.1

ORF Insert Sequence:

ATGTCGGTCA GCGAGCTCTA CGGCCAGTGC ACGAGGGTCT GGATCCCTGA CCCTGATGAA 
GTGTGGCGCT CAGCTGAGTT AACCAAGGAC TACAAGGAGG GAGACACGAG CCTCCAGCTC 
AGACTGGAAG ATGAAACGAT TCGGGAATAC CCTATTGATG TCCAAAGCAA CCAGCTGCCC 
TTTTTGCGGA ATCCAGACAT CTTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTA 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGTTTCC TGGAGTCGAA CCACATCTAC 
ACTTACTGTG GTATCGTGCT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGGG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGCGGGG AGTCTGGAGC CGGCAAGACT GTGTCAGCCA AGTACGCCAT GCGCTATTTT 
GCCACGGTTG GTGGCTCAGC CAGTGACACC AACATTGAAG AGAAGGTCCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCTAAA ACCACTCGCA ATGACAACAG CAGCCGTTTT 
GGGAAGTACA TTCAGATTGG CTTTGATAAA AGGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACCTGT TGGAGAAGTC CAGAGTGGTC TTTCAGGCAG ATGACGAGCG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCCGCCAGT CTCCCGGAAT TTAAGGAGCT TGCACTGACA 
TGCGCAGAGG ACTTCTTCTA TACCTCACAG GGAAGAGACA CGTGTATCGA GGGTGTCGAT 
GATGCCGAGG ACTTTGAGAA GACGCGGCAA GCCTTCACAC TCCTCGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCTATCCTGC ACCTTGGAAA TGTGGAGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCTC AGGATGAACA CTTGAACAGC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTATG CCATCGAAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACTATGTCCC TGCAGCAGGT GGTCAACGCC 
CGCAACGCCC TAGCCAAGCA CATCTATGCC CAGCTGTTCG GCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACGCC TCTCAAGCAA CACTCCTTCA TTGGCGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT GAACAGCTTT GAGCAGTTCT GTATCAACTA TGCGAATGAG 
AAGCTACAGC AGCAGTTCAA CTCGCACGTG TTCAAACTAG AGCAAGAAGA ATATATGAAG 
GAGCAGATAC CTTGGACCTT GATTGATTTT TACGATAACC AACCTTGTAT TGACCTCATA 
GAAGCTAAAC TGGGCATCTT GGACCTGTTG GACGAAGAGT GTAAGGTCCC TAAAGGAACT 
GACCAGAACT GGGCACAGAA GCTCTATGAC CGGCACTCTG GCAGCCAGCA CTTCCAGAAA 
CCCCGCATGT CCAACACAGC CTTCATTGTG GTCCACTTTG CTGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA GAACAGAGAC ACGGTGTATG AAGAGCAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAT TTGTTTCATG ATGACAAAGA CTCTGTTCCT 
GCCACCACTG CATCTGGGAA GGGCTCATCC TCAAAGATCA ACATTCGCTC TGCCAGACCC 
CCTCTGAAAG TCTCCAACAA GGAACACAAG AAAACCGTGG GCCACCAGTT CCGTACCTCC 
CTGCATCTGC TCATGGAGAC CCTGAATGCC ACGACCCCAC ACTACGTGCG CTGCATCAAG 
CCAAATGACG AGAAGCTCCC CTTCCACTTT GACCCAAAGA GAGCAGTGCA GCAGCTCAGA 
GCGTGTGGGG TGTTGGAGAC CATCCGAATC AGTGCAGCCG GCTACCCATC CAGGTGGGCC 
TACCACGACT TCTTCAACCG GTATCGGGTG CTGGTCAAGA AGAGAGAGCT CGCCAACACA 
GACAAAAAGG CCATCTGCAG ATCTGTCCTG GAAAGCCTCA TCAAGGATCC CGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTTTTCCGG GCAGGCCAGG TGGCTTACCT GGAGAAGCTT 
CGGGCTGACA AGTTCCGGGC AGCCACGATC ATGATCCAGA AAACTGTCCG GGGCTGGCTG 
CAGAAGGTGA AATACCGCAG GCTGAAGGCC ACTACCTTAA CTCTGCAGCG GTATTGCCGA 
GGGCTCCTGG CCCGAAGGCT GGCCGAGCAT CTGCGGAGGA CGCGAGCAGC CATCATGCTC 
CAGAAGCAGT ACCGCATGCG GAGGGCCCGC CTGGCCTACC AGAGGGTCCA CAGGGCCACC 
GTCACCATCC AGGCCTTCAC TCGGGGCATG TTCGTGCGGA GAATCTACCA GCAGGTCCTC 
ATGGAGCACA AGGCCACCAT CCTCCAGAAG CACGTGCGGG GCTGGGTGGC ACGTAGGCGC 
TTTCAGCGGC TGCGGGGCGC GGCCATCGTC ATCCAGTGTG CCTTCCGCAT GCTTAAGGCC 
AAGCAGGAGC TGAAGGCCCT CAAGATCGAG GCCCGCTCAG CAGAGCACCT GAAACGTCTC 
AACGTGGGCA TGGAGAACAA GGTGGTCCAG CTGCAGCGGA AGATTGACGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCGGA GCAGTTGTCG GCGGTCACCT CCACACACAC CATGGAGGTA 
GAGAAGTTGA AGAAGGAGCT GGCCCGCTAC CAGCAGAGCC AAGGTGAGGA CGGCAGCCTG 
AGGCTGCAGG AGGAGGTGGA GAGCCTGCGG GCGGAGCTGC AGAGGGCCCA CTCGGAGCGC 
AAGATCCTGG AGGACGCCCA CACCAAGGAG AAGGACGAGC TGAGAAAGCG CGTTGCAGAC 
CTGGAGGAAG AAAATGCTCT CTTAAAAGAT GAGAAAGAAC AGCTTAACAA CCAAATCCTG 
TGCCAAGCAA AAGACGAATC TGCCCAAAAC TCTGCCAAGG AAAATCTGAT GAAGAGGGAG 
CTGGAGGAGG AGCGCTCCCG GTACCAGAAC CTCGTGAAGG AATATTCACG GTTGGAGCAG 
AGATACGACA ACCTTCGGGA TGAGATGACC ATCATCAAGC AAACCCCAGG GCACAGGCGG 
AACCCATCAA ACCAGAGCAG TTTAGAATCT GACTCCAATT ACCCCTCCAT CTCCACATCT 
GAGGTCGGAG ACACAGAGGA TGCCCTCCAA CAGGTGGAGG ACATCGGCCT GGAAAAGGCA 
GCCATGGACA TGACCGTCTT CCTGAAACTG CAGAAGAGAG TGCGCGAGCT GGAGCAGGAG 
AGGAAGAAAC TGCAGGTGCA GCTGGAGAAG AGGGAGCAGC AGGACAGCCG GAAGGTCCAG 
GTGGAACAAG CAAAGAATGA CCTGGATTTG GACCAGGATG CAGATCTAGC CTACAACAGT 
CTGAAGAGGC AAGAGCTAGA GTCGGAGAAC AAGAAGCTGA AGAACGACCT GAACGAGCTG 
AGGAAAGCCG TGGCTGACCA AGCCACACAG AACAACTCCA GCCACAGCTC CCCCGACAGC 
TACAGCCTGC TGCTGAACCA GCTCAAGCTG GCCAACGAGG AACTTGAGGT TCGCAAGGAG 
GAGGTGCTCA TCCTGAGGAC GCAGATCGTC AGCGCCGACC AGCGCCGACT CGCCGGCAAG 
AGCACGGAGC CGAACATTAA TGCCAGAACA AGTTGGCCTA ACAGTGAGAA GCATGTAGAT 
CAGGAAGATG CCATCGAGGC CTATCATGGG GTCTGCCAGA CAAACAGCAA GACTGAGGAT 
TGGGGATATT TGAATGAAGA TGGAGAGCTC GGCTTGGCCT ACCAAGGCCT AAAGCAAGTT 
GCCAGGTTGC TGGAGGCCCA GCTGCAGGCC CAGAGCCGCG AGCATGAGGA GGAGGTGGAG 
GGGCTCCGGG CCCAGGTGGA AGCCCTGAAG GAGGAACTGG ACAAGCAGCA GCAGACCTTC 
TGCCAGACGC TGCTGCTCTC CCCAGAGGCC CAGGTGGAAT TTGGCGTCCA GCAGGAGATA 
TCCCGGCTCA CCAATGAGAA CCTGGACCTT AAAGAACTGG TAGAAAAGCT GGAAAAGAAT 
GAGAGGAAGC TCAAGAAGCA GCTGAAGATT TACATGAAGA AAGTCCAGGA CCTAGAAGCT 
GCCCAGGCGC TGGCCCAGAG TGAGAGGAGA CGCCATGAAC TCAACAGGCA GGTCACTGTG 
CAGCGGAAGG AGAAGGACTT CCAGGGCATG CTGGAGTACC ACAAGGAAGA TGAGGCCCTT 
CTCATTCGGA ACCTGGTGAC AGACCTGAAG CCGCAGGCGC TGGCGGGCAC GGTGCCCTGT 
CTTCCTGCCT ACATTCTCTA CATGTGCATC AGGCACGCAG ATTACGTCAA CGACGACCTC 
AAGGTGCACT CCTTGCTGAC CTCCACCATC AACGGCATTA AGAAAGTCCT GAAGAAGCAC 
AATGATGACT TCGAGATGAC ATCATTCTGG TTATCCAATA CCTGCCGCCT CCTTCATTGT 
TTGAAGCAGT ATAGCGGGGA TGAGGGCTTC ATGACTCAGA ATACTGCCAA ACAGAATGAG 
CACTGTCTTA AGAACTTTGA CCTCACTGAA TACCGCCAGG TGCTGAGTGA CCTTTCCATT 
CAAATCTACC AGCAACTCAT TAAAATCGCC GAGGGTGTGT TGCAGCCTAT GATAGTTTCT 
GCCATGTTGG AAAATGAGAG CATTCAGGGT CTATCTGGTG TGAAGCCAAC AGGCTACCGC 
AAGCGCTCCT CCAGCATGGC GGACGGGGAC AACTCCTACT GCCTGGAAGC CGTCATCCGC 
CAGATGAATT CCTTTCACAC AGTCATGTGT GACCAGGGCC TGGACCCGGA GATCATCCTG 
CAGGTGTTCA AGCAGCTCTT CTACATGATC AACGCTGTGA CTCTCAACAA CCTGCTCCTG 
CGGAAGGACG TCTGCTCTTG GAGCACAGGC ATGCAGCTCA GGTACAATAT AAGTCAACTT 
GAAGAGTGGC TTCGGGGAAG AAACCTTCAC CAGAGCGGAG CAGTCCAGAC CATGGAACCT 
CTGATCCAAG CAGCCCAGCT CCTACAGTTA AAGAAGAAAA CCCCTGAGGA CGCAGAGGCC 
ATCTGCTCGC TGTGCACCTC CCTCAGCACC CAGCAGATTG TCAAAATTTT AAACCTTTAT 
ACTCCCCTGA ATGAATTTGA AGAACGGGTA ACCGTGGCCT TTATACGAAC AATCCAGGCA 
CAACTACAAG AGCGGAATGA CCCTCAGCAA CTGCTGTTAG ACTTCAAGCA CATGTTTCCA 
GTGTTGTTTC CATTTAACCC ATCCTCTCTG ACCATGGACT CCATCCACAT CCCAGCATAT 
CTCAATCTGG AGTTCCTCAA TGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO5B ( XM_023241983.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023097751.1 Felis catus myosin VB (MYO5B), transcript variant X2, mRNA.

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CloneID	OFb63290
Clone ID Related Accession (Same CDS sequence)	XM_023241983.1
Accession Version	XM_023241983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCGAGCTCTA CGGCCAGTGC ACGAGGGTCT GGATCCCTGA CCCTGATGAA 
GTGTGGCGCT CAGCTGAGTT AACCAAGGAC TACAAGGAGG GAGACACGAG CCTCCAGCTC 
AGACTGGAAG ATGAAACGAT TCGGGAATAC CCTATTGATG TCCAAAGCAA CCAGCTGCCC 
TTTTTGCGGA ATCCAGACAT CTTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTA 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGTTTCC TGGAGTCGAA CCACATCTAC 
ACTTACTGTG GTATCGTGCT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGGG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGCGGGG AGTCTGGAGC CGGCAAGACT GTGTCAGCCA AGTACGCCAT GCGCTATTTT 
GCCACGGTTG GTGGCTCAGC CAGTGACACC AACATTGAAG AGAAGGTCCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCTAAA ACCACTCGCA ATGACAACAG CAGCCGTTTT 
GGGAAGTACA TTCAGATTGG CTTTGATAAA AGGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACCTGT TGGAGAAGTC CAGAGTGGTC TTTCAGGCAG ATGACGAGCG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCCGCCAGT CTCCCGGAAT TTAAGGAGCT TGCACTGACA 
TGCGCAGAGG ACTTCTTCTA TACCTCACAG GGAAGAGACA CGTGTATCGA GGGTGTCGAT 
GATGCCGAGG ACTTTGAGAA GACGCGGCAA GCCTTCACAC TCCTCGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCTATCCTGC ACCTTGGAAA TGTGGAGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCTC AGGATGAACA CTTGAACAGC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTATG CCATCGAAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACTATGTCCC TGCAGCAGGT GGTCAACGCC 
CGCAACGCCC TAGCCAAGCA CATCTATGCC CAGCTGTTCG GCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACGCC TCTCAAGCAA CACTCCTTCA TTGGCGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT GAACAGCTTT GAGCAGTTCT GTATCAACTA TGCGAATGAG 
AAGCTACAGC AGCAGTTCAA CTCGCACGTG TTCAAACTAG AGCAAGAAGA ATATATGAAG 
GAGCAGATAC CTTGGACCTT GATTGATTTT TACGATAACC AACCTTGTAT TGACCTCATA 
GAAGCTAAAC TGGGCATCTT GGACCTGTTG GACGAAGAGT GTAAGGTCCC TAAAGGAACT 
GACCAGAACT GGGCACAGAA GCTCTATGAC CGGCACTCTG GCAGCCAGCA CTTCCAGAAA 
CCCCGCATGT CCAACACAGC CTTCATTGTG GTCCACTTTG CTGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA GAACAGAGAC ACGGTGTATG AAGAGCAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAT TTGTTTCATG ATGACAAAGA CTCTGTTCCT 
GCCACCACTG CATCTGGGAA GGGCTCATCC TCAAAGATCA ACATTCGCTC TGCCAGACCC 
CCTCTGAAAG TCTCCAACAA GGAACACAAG AAAACCGTGG GCCACCAGTT CCGTACCTCC 
CTGCATCTGC TCATGGAGAC CCTGAATGCC ACGACCCCAC ACTACGTGCG CTGCATCAAG 
CCAAATGACG AGAAGCTCCC CTTCCACTTT GACCCAAAGA GAGCAGTGCA GCAGCTCAGA 
GCGTGTGGGG TGTTGGAGAC CATCCGAATC AGTGCAGCCG GCTACCCATC CAGGTGGGCC 
TACCACGACT TCTTCAACCG GTATCGGGTG CTGGTCAAGA AGAGAGAGCT CGCCAACACA 
GACAAAAAGG CCATCTGCAG ATCTGTCCTG GAAAGCCTCA TCAAGGATCC CGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTTTTCCGG GCAGGCCAGG TGGCTTACCT GGAGAAGCTT 
CGGGCTGACA AGTTCCGGGC AGCCACGATC ATGATCCAGA AAACTGTCCG GGGCTGGCTG 
CAGAAGGTGA AATACCGCAG GCTGAAGGCC ACTACCTTAA CTCTGCAGCG GTATTGCCGA 
GGGCTCCTGG CCCGAAGGCT GGCCGAGCAT CTGCGGAGGA CGCGAGCAGC CATCATGCTC 
CAGAAGCAGT ACCGCATGCG GAGGGCCCGC CTGGCCTACC AGAGGGTCCA CAGGGCCACC 
GTCACCATCC AGGCCTTCAC TCGGGGCATG TTCGTGCGGA GAATCTACCA GCAGGTCCTC 
ATGGAGCACA AGGCCACCAT CCTCCAGAAG CACGTGCGGG GCTGGGTGGC ACGTAGGCGC 
TTTCAGCGGC TGCGGGGCGC GGCCATCGTC ATCCAGTGTG CCTTCCGCAT GCTTAAGGCC 
AAGCAGGAGC TGAAGGCCCT CAAGATCGAG GCCCGCTCAG CAGAGCACCT GAAACGTCTC 
AACGTGGGCA TGGAGAACAA GGTGGTCCAG CTGCAGCGGA AGATTGACGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCGGA GCAGTTGTCG GCGGTCACCT CCACACACAC CATGGAGGTA 
GAGAAGTTGA AGAAGGAGCT GGCCCGCTAC CAGCAGAGCC AAGGTGAGGA CGGCAGCCTG 
AGGCTGCAGG AGGAGGTGGA GAGCCTGCGG GCGGAGCTGC AGAGGGCCCA CTCGGAGCGC 
AAGATCCTGG AGGACGCCCA CACCAAGGAG AAGGACGAGC TGAGAAAGCG CGTTGCAGAC 
CTGGAGGAAG AAAATGCTCT CTTAAAAGAT GAGAAAGAAC AGCTTAACAA CCAAATCCTG 
TGCCAAGCAA AAGACGAATC TGCCCAAAAC TCTGCCAAGG AAAATCTGAT GAAGAGGGAG 
CTGGAGGAGG AGCGCTCCCG GTACCAGAAC CTCGTGAAGG AATATTCACG GTTGGAGCAG 
AGATACGACA ACCTTCGGGA TGAGATGACC ATCATCAAGC AAACCCCAGG GCACAGGCGG 
AACCCATCAA ACCAGAGCAG TTTAGAATCT GACTCCAATT ACCCCTCCAT CTCCACATCT 
GAGGTCGGAG ACACAGAGGA TGCCCTCCAA CAGGTGGAGG ACATCGGCCT GGAAAAGGCA 
GCCATGGACA TGACCGTCTT CCTGAAACTG CAGAAGAGAG TGCGCGAGCT GGAGCAGGAG 
AGGAAGAAAC TGCAGGTGCA GCTGGAGAAG AGGGAGCAGC AGGACAGCCG GAAGGTCCAG 
GTGGAACAAG CAAAGAATGA CCTGGATTTG GACCAGGATG CAGATCTAGC CTACAACAGT 
CTGAAGAGGC AAGAGCTAGA GTCGGAGAAC AAGAAGCTGA AGAACGACCT GAACGAGCTG 
AGGAAAGCCG TGGCTGACCA AGCCACACAG AACAACTCCA GCCACAGCTC CCCCGACAGC 
TACAGCCTGC TGCTGAACCA GCTCAAGCTG GCCAACGAGG AACTTGAGGT TCGCAAGGAG 
GAGGTGCTCA TCCTGAGGAC GCAGATCGTC AGCGCCGACC AGCGCCGACT CGCCGGCAAG 
AGCACGGAGC CGAACATTAA TGCCAGAACA AGTTGGCCTA ACAGTGAGAA GCATGTAGAT 
CAGGAAGATG CCATCGAGGC CTATCATGGG GTCTGCCAGA CAAACAGGTT GCTGGAGGCC 
CAGCTGCAGG CCCAGAGCCG CGAGCATGAG GAGGAGGTGG AGGGGCTCCG GGCCCAGGTG 
GAAGCCCTGA AGGAGGAACT GGACAAGCAG CAGCAGACCT TCTGCCAGAC GCTGCTGCTC 
TCCCCAGAGG CCCAGGTGGA ATTTGGCGTC CAGCAGGAGA TATCCCGGCT CACCAATGAG 
AACCTGGACC TTAAAGAACT GGTAGAAAAG CTGGAAAAGA ATGAGAGGAA GCTCAAGAAG 
CAGCTGAAGA TTTACATGAA GAAAGTCCAG GACCTAGAAG CTGCCCAGGC GCTGGCCCAG 
AGTGAGAGGA GACGCCATGA ACTCAACAGG CAGGTCACTG TGCAGCGGAA GGAGAAGGAC 
TTCCAGGGCA TGCTGGAGTA CCACAAGGAA GATGAGGCCC TTCTCATTCG GAACCTGGTG 
ACAGACCTGA AGCCGCAGGC GCTGGCGGGC ACGGTGCCCT GTCTTCCTGC CTACATTCTC 
TACATGTGCA TCAGGCACGC AGATTACGTC AACGACGACC TCAAGGTGCA CTCCTTGCTG 
ACCTCCACCA TCAACGGCAT TAAGAAAGTC CTGAAGAAGC ACAATGATGA CTTCGAGATG 
ACATCATTCT GGTTATCCAA TACCTGCCGC CTCCTTCATT GTTTGAAGCA GTATAGCGGG 
GATGAGGGCT TCATGACTCA GAATACTGCC AAACAGAATG AGCACTGTCT TAAGAACTTT 
GACCTCACTG AATACCGCCA GGTGCTGAGT GACCTTTCCA TTCAAATCTA CCAGCAACTC 
ATTAAAATCG CCGAGGGTGT GTTGCAGCCT ATGATAGTTT CTGCCATGTT GGAAAATGAG 
AGCATTCAGG GTCTATCTGG TGTGAAGCCA ACAGGCTACC GCAAGCGCTC CTCCAGCATG 
GCGGACGGGG ACAACTCCTA CTGCCTGGAA GCCGTCATCC GCCAGATGAA TTCCTTTCAC 
ACAGTCATGT GTGACCAGGG CCTGGACCCG GAGATCATCC TGCAGGTGTT CAAGCAGCTC 
TTCTACATGA TCAACGCTGT GACTCTCAAC AACCTGCTCC TGCGGAAGGA CGTCTGCTCT 
TGGAGCACAG GCATGCAGCT CAGGTACAAT ATAAGTCAAC TTGAAGAGTG GCTTCGGGGA 
AGAAACCTTC ACCAGAGCGG AGCAGTCCAG ACCATGGAAC CTCTGATCCA AGCAGCCCAG 
CTCCTACAGT TAAAGAAGAA AACCCCTGAG GACGCAGAGG CCATCTGCTC GCTGTGCACC 
TCCCTCAGCA CCCAGCAGAT TGTCAAAATT TTAAACCTTT ATACTCCCCT GAATGAATTT 
GAAGAACGGG TAACCGTGGC CTTTATACGA ACAATCCAGG CACAACTACA AGAGCGGAAT 
GACCCTCAGC AACTGCTGTT AGACTTCAAG CACATGTTTC CAGTGTTGTT TCCATTTAAC 
CCATCCTCTC TGACCATGGA CTCCATCCAC ATCCCAGCAT ATCTCAATCT GGAGTTCCTC 
AATGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023097751.1
CDS	404..5875
Translation	MSVSELYGQCTRVWIPDPDEVWRSAELTKDYKEGDTSLQLRLEDETIREYPIDVQSNQLPFLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTSQGRDTCIEGVDDAEDFEKTRQAFTLLGVRESHQISIFKIIASILHLGNVEIQAERDGDSCSISPQDEHLNSFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARNALAKHIYAQLFGWIVEHINKALHTPLKQHSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHSGSQHFQKPRMSNTAFIVVHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFHDDKDSVPATTASGKGSSSKINIRSARPPLKVSNKEHKKTVGHQFRTSLHLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVKKRELANTDKKAICRSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQKVKYRRLKATTLTLQRYCRGLLARRLAEHLRRTRAAIMLQKQYRMRRARLAYQRVHRATVTIQAFTRGMFVRRIYQQVLMEHKATILQKHVRGWVARRRFQRLRGAAIVIQCAFRMLKAKQELKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSTHTMEVEKLKKELARYQQSQGEDGSLRLQEEVESLRAELQRAHSERKILEDAHTKEKDELRKRVADLEEENALLKDEKEQLNNQILCQAKDESAQNSAKENLMKRELEEERSRYQNLVKEYSRLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEVGDTEDALQQVEDIGLEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKREQQDSRKVQVEQAKNDLDLDQDADLAYNSLKRQELESENKKLKNDLNELRKAVADQATQNNSSHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIVSADQRRLAGKSTEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNRLLEAQLQAQSREHEEEVEGLRAQVEALKEELDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDLKELVEKLEKNERKLKKQLKIYMKKVQDLEAAQALAQSERRRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQALAGTVPCLPAYILYMCIRHADYVNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAVIRQMNSFHTVMCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGRNLHQSGAVQTMEPLIQAAQLLQLKKKTPEDAEAICSLCTSLSTQQIVKILNLYTPLNEFEERVTVAFIRTIQAQLQERNDPQQLLLDFKHMFPVLFPFNPSSLTMDSIHIPAYLNLEFLNEV

Target ORF information:

RefSeq Version	XM_023241983.1
Organism	Felis catus(domestic cat)
Definition	Felis catus myosin VB (MYO5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023241983.1

ORF Insert Sequence:

ATGTCGGTCA GCGAGCTCTA CGGCCAGTGC ACGAGGGTCT GGATCCCTGA CCCTGATGAA 
GTGTGGCGCT CAGCTGAGTT AACCAAGGAC TACAAGGAGG GAGACACGAG CCTCCAGCTC 
AGACTGGAAG ATGAAACGAT TCGGGAATAC CCTATTGATG TCCAAAGCAA CCAGCTGCCC 
TTTTTGCGGA ATCCAGACAT CTTGGTGGGA GAAAATGACC TAACTGCCCT TAGCTATCTA 
CATGAGCCTG CAGTTTTGCA TAATTTAAAG GTCCGTTTCC TGGAGTCGAA CCACATCTAC 
ACTTACTGTG GTATCGTGCT TGTTGCCATT AATCCTTATG AACAGTTGCC AATCTATGGT 
CAAGATGTCA TCTATGCCTA CAGTGGCCAA AACATGGGGG ACATGGACCC CCACATCTTT 
GCTGTGGCAG AAGAAGCCTA CAAGCAGATG GCCAGAGATG AGAAGAACCA GTCCATCATA 
GTCAGCGGGG AGTCTGGAGC CGGCAAGACT GTGTCAGCCA AGTACGCCAT GCGCTATTTT 
GCCACGGTTG GTGGCTCAGC CAGTGACACC AACATTGAAG AGAAGGTCCT GGCATCCAGT 
CCCATCATGG AGGCCATTGG AAATGCTAAA ACCACTCGCA ATGACAACAG CAGCCGTTTT 
GGGAAGTACA TTCAGATTGG CTTTGATAAA AGGTACCACA TCATTGGGGC CAACATGAGG 
ACCTACCTGT TGGAGAAGTC CAGAGTGGTC TTTCAGGCAG ATGACGAGCG GAATTACCAC 
ATCTTTTACC AGCTCTGTGC TGCCGCCAGT CTCCCGGAAT TTAAGGAGCT TGCACTGACA 
TGCGCAGAGG ACTTCTTCTA TACCTCACAG GGAAGAGACA CGTGTATCGA GGGTGTCGAT 
GATGCCGAGG ACTTTGAGAA GACGCGGCAA GCCTTCACAC TCCTCGGAGT GAGAGAGTCC 
CATCAGATAA GCATTTTTAA GATAATTGCT TCTATCCTGC ACCTTGGAAA TGTGGAGATT 
CAGGCTGAGC GTGATGGTGA TTCCTGTAGT ATATCACCTC AGGATGAACA CTTGAACAGC 
TTCTGCCGAC TGCTAGGGGT GGAGCACAGT CAGATGGAGC ACTGGCTATG CCATCGAAAG 
CTGGTCACCA CCTCAGAGAC CTACGTCAAG ACTATGTCCC TGCAGCAGGT GGTCAACGCC 
CGCAACGCCC TAGCCAAGCA CATCTATGCC CAGCTGTTCG GCTGGATTGT GGAGCACATC 
AACAAAGCCC TGCACACGCC TCTCAAGCAA CACTCCTTCA TTGGCGTCCT GGACATCTAT 
GGGTTTGAGA CATTTGAGGT GAACAGCTTT GAGCAGTTCT GTATCAACTA TGCGAATGAG 
AAGCTACAGC AGCAGTTCAA CTCGCACGTG TTCAAACTAG AGCAAGAAGA ATATATGAAG 
GAGCAGATAC CTTGGACCTT GATTGATTTT TACGATAACC AACCTTGTAT TGACCTCATA 
GAAGCTAAAC TGGGCATCTT GGACCTGTTG GACGAAGAGT GTAAGGTCCC TAAAGGAACT 
GACCAGAACT GGGCACAGAA GCTCTATGAC CGGCACTCTG GCAGCCAGCA CTTCCAGAAA 
CCCCGCATGT CCAACACAGC CTTCATTGTG GTCCACTTTG CTGATAAGGT GGAGTACCTT 
TCAGATGGTT TTCTGGAGAA GAACAGAGAC ACGGTGTATG AAGAGCAGAT CAACATCCTG 
AAGGCCAGCA AGTTCCCACT AGTGGCTGAT TTGTTTCATG ATGACAAAGA CTCTGTTCCT 
GCCACCACTG CATCTGGGAA GGGCTCATCC TCAAAGATCA ACATTCGCTC TGCCAGACCC 
CCTCTGAAAG TCTCCAACAA GGAACACAAG AAAACCGTGG GCCACCAGTT CCGTACCTCC 
CTGCATCTGC TCATGGAGAC CCTGAATGCC ACGACCCCAC ACTACGTGCG CTGCATCAAG 
CCAAATGACG AGAAGCTCCC CTTCCACTTT GACCCAAAGA GAGCAGTGCA GCAGCTCAGA 
GCGTGTGGGG TGTTGGAGAC CATCCGAATC AGTGCAGCCG GCTACCCATC CAGGTGGGCC 
TACCACGACT TCTTCAACCG GTATCGGGTG CTGGTCAAGA AGAGAGAGCT CGCCAACACA 
GACAAAAAGG CCATCTGCAG ATCTGTCCTG GAAAGCCTCA TCAAGGATCC CGACAAGTTC 
CAGTTTGGCC GCACCAAGAT CTTTTTCCGG GCAGGCCAGG TGGCTTACCT GGAGAAGCTT 
CGGGCTGACA AGTTCCGGGC AGCCACGATC ATGATCCAGA AAACTGTCCG GGGCTGGCTG 
CAGAAGGTGA AATACCGCAG GCTGAAGGCC ACTACCTTAA CTCTGCAGCG GTATTGCCGA 
GGGCTCCTGG CCCGAAGGCT GGCCGAGCAT CTGCGGAGGA CGCGAGCAGC CATCATGCTC 
CAGAAGCAGT ACCGCATGCG GAGGGCCCGC CTGGCCTACC AGAGGGTCCA CAGGGCCACC 
GTCACCATCC AGGCCTTCAC TCGGGGCATG TTCGTGCGGA GAATCTACCA GCAGGTCCTC 
ATGGAGCACA AGGCCACCAT CCTCCAGAAG CACGTGCGGG GCTGGGTGGC ACGTAGGCGC 
TTTCAGCGGC TGCGGGGCGC GGCCATCGTC ATCCAGTGTG CCTTCCGCAT GCTTAAGGCC 
AAGCAGGAGC TGAAGGCCCT CAAGATCGAG GCCCGCTCAG CAGAGCACCT GAAACGTCTC 
AACGTGGGCA TGGAGAACAA GGTGGTCCAG CTGCAGCGGA AGATTGACGA CCAGAACAAA 
GAGTTCAAGA CTCTGTCGGA GCAGTTGTCG GCGGTCACCT CCACACACAC CATGGAGGTA 
GAGAAGTTGA AGAAGGAGCT GGCCCGCTAC CAGCAGAGCC AAGGTGAGGA CGGCAGCCTG 
AGGCTGCAGG AGGAGGTGGA GAGCCTGCGG GCGGAGCTGC AGAGGGCCCA CTCGGAGCGC 
AAGATCCTGG AGGACGCCCA CACCAAGGAG AAGGACGAGC TGAGAAAGCG CGTTGCAGAC 
CTGGAGGAAG AAAATGCTCT CTTAAAAGAT GAGAAAGAAC AGCTTAACAA CCAAATCCTG 
TGCCAAGCAA AAGACGAATC TGCCCAAAAC TCTGCCAAGG AAAATCTGAT GAAGAGGGAG 
CTGGAGGAGG AGCGCTCCCG GTACCAGAAC CTCGTGAAGG AATATTCACG GTTGGAGCAG 
AGATACGACA ACCTTCGGGA TGAGATGACC ATCATCAAGC AAACCCCAGG GCACAGGCGG 
AACCCATCAA ACCAGAGCAG TTTAGAATCT GACTCCAATT ACCCCTCCAT CTCCACATCT 
GAGGTCGGAG ACACAGAGGA TGCCCTCCAA CAGGTGGAGG ACATCGGCCT GGAAAAGGCA 
GCCATGGACA TGACCGTCTT CCTGAAACTG CAGAAGAGAG TGCGCGAGCT GGAGCAGGAG 
AGGAAGAAAC TGCAGGTGCA GCTGGAGAAG AGGGAGCAGC AGGACAGCCG GAAGGTCCAG 
GTGGAACAAG CAAAGAATGA CCTGGATTTG GACCAGGATG CAGATCTAGC CTACAACAGT 
CTGAAGAGGC AAGAGCTAGA GTCGGAGAAC AAGAAGCTGA AGAACGACCT GAACGAGCTG 
AGGAAAGCCG TGGCTGACCA AGCCACACAG AACAACTCCA GCCACAGCTC CCCCGACAGC 
TACAGCCTGC TGCTGAACCA GCTCAAGCTG GCCAACGAGG AACTTGAGGT TCGCAAGGAG 
GAGGTGCTCA TCCTGAGGAC GCAGATCGTC AGCGCCGACC AGCGCCGACT CGCCGGCAAG 
AGCACGGAGC CGAACATTAA TGCCAGAACA AGTTGGCCTA ACAGTGAGAA GCATGTAGAT 
CAGGAAGATG CCATCGAGGC CTATCATGGG GTCTGCCAGA CAAACAGGTT GCTGGAGGCC 
CAGCTGCAGG CCCAGAGCCG CGAGCATGAG GAGGAGGTGG AGGGGCTCCG GGCCCAGGTG 
GAAGCCCTGA AGGAGGAACT GGACAAGCAG CAGCAGACCT TCTGCCAGAC GCTGCTGCTC 
TCCCCAGAGG CCCAGGTGGA ATTTGGCGTC CAGCAGGAGA TATCCCGGCT CACCAATGAG 
AACCTGGACC TTAAAGAACT GGTAGAAAAG CTGGAAAAGA ATGAGAGGAA GCTCAAGAAG 
CAGCTGAAGA TTTACATGAA GAAAGTCCAG GACCTAGAAG CTGCCCAGGC GCTGGCCCAG 
AGTGAGAGGA GACGCCATGA ACTCAACAGG CAGGTCACTG TGCAGCGGAA GGAGAAGGAC 
TTCCAGGGCA TGCTGGAGTA CCACAAGGAA GATGAGGCCC TTCTCATTCG GAACCTGGTG 
ACAGACCTGA AGCCGCAGGC GCTGGCGGGC ACGGTGCCCT GTCTTCCTGC CTACATTCTC 
TACATGTGCA TCAGGCACGC AGATTACGTC AACGACGACC TCAAGGTGCA CTCCTTGCTG 
ACCTCCACCA TCAACGGCAT TAAGAAAGTC CTGAAGAAGC ACAATGATGA CTTCGAGATG 
ACATCATTCT GGTTATCCAA TACCTGCCGC CTCCTTCATT GTTTGAAGCA GTATAGCGGG 
GATGAGGGCT TCATGACTCA GAATACTGCC AAACAGAATG AGCACTGTCT TAAGAACTTT 
GACCTCACTG AATACCGCCA GGTGCTGAGT GACCTTTCCA TTCAAATCTA CCAGCAACTC 
ATTAAAATCG CCGAGGGTGT GTTGCAGCCT ATGATAGTTT CTGCCATGTT GGAAAATGAG 
AGCATTCAGG GTCTATCTGG TGTGAAGCCA ACAGGCTACC GCAAGCGCTC CTCCAGCATG 
GCGGACGGGG ACAACTCCTA CTGCCTGGAA GCCGTCATCC GCCAGATGAA TTCCTTTCAC 
ACAGTCATGT GTGACCAGGG CCTGGACCCG GAGATCATCC TGCAGGTGTT CAAGCAGCTC 
TTCTACATGA TCAACGCTGT GACTCTCAAC AACCTGCTCC TGCGGAAGGA CGTCTGCTCT 
TGGAGCACAG GCATGCAGCT CAGGTACAAT ATAAGTCAAC TTGAAGAGTG GCTTCGGGGA 
AGAAACCTTC ACCAGAGCGG AGCAGTCCAG ACCATGGAAC CTCTGATCCA AGCAGCCCAG 
CTCCTACAGT TAAAGAAGAA AACCCCTGAG GACGCAGAGG CCATCTGCTC GCTGTGCACC 
TCCCTCAGCA CCCAGCAGAT TGTCAAAATT TTAAACCTTT ATACTCCCCT GAATGAATTT 
GAAGAACGGG TAACCGTGGC CTTTATACGA ACAATCCAGG CACAACTACA AGAGCGGAAT 
GACCCTCAGC AACTGCTGTT AGACTTCAAG CACATGTTTC CAGTGTTGTT TCCATTTAAC 
CCATCCTCTC TGACCATGGA CTCCATCCAC ATCCCAGCAT ATCTCAATCT GGAGTTCCTC 
AATGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MYO5B cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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