SPTBN1 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTBN1
Entrez Gene ID	101090509
Full Name	spectrin beta, non-erythrocytic 1
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011281013.2	XP_011279315.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_023251678.1	XP_023107446.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_003983998.5	XP_003984047.1	spectrin beta chain, non-erythrocytic 1 isoform X3
XM_019827200.1	XP_019682759.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_023251677.1	XP_023107445.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_023251679.1	XP_023107447.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_003983998.4	XP_003984047.1	spectrin beta chain, non-erythrocytic 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb04435	XM_003983998.5 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb29929	XM_011281013.2 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40307	XM_019827200.1 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb73568	XM_023251677.1 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb73568	XM_023251678.1 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb73569	XM_023251679.1 Latest version!	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OFb04435	XM_003983998.4	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTBN1 ( XM_003983998.5 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984047.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb04435
Clone ID Related Accession (Same CDS sequence)	XM_003983998.5 , XM_003983998.4
Accession Version	XM_003983998.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003983998.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984047.1
CDS	445..6912
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL

Target ORF information:

RefSeq Version	XM_003983998.5
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003983998.5

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_003983998.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984047.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

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CloneID	OFb04435
Clone ID Related Accession (Same CDS sequence)	XM_003983998.5 , XM_003983998.4
Accession Version	XM_003983998.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003983998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984047.1
CDS	449..6916
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL

Target ORF information:

RefSeq Version	XM_003983998.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003983998.4

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_011281013.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_011279315.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

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CloneID	OFb29929
Clone ID Related Accession (Same CDS sequence)	XM_011281013.2
Accession Version	XM_011281013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011281013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011279315.1
CDS	147..6983
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRMAEAVDTSEMVNGAAEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLR

Target ORF information:

RefSeq Version	XM_011281013.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011281013.2

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_019827200.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682759.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

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CloneID	OFb40307
Clone ID Related Accession (Same CDS sequence)	XM_019827200.1
Accession Version	XM_019827200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GATGGCAGAA GCGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCCGCAGAGC AAAGAACAAG CTCAAAGGAG TCTAGCCCCA TCCCCTCCCC CACGTCCGAC 
CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCTCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACCGCT GCCTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC 
GTGTTCAAGC TAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682759.1
CDS	448..7245
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRMAEAVDTSEMVNGAAEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLR

Target ORF information:

RefSeq Version	XM_019827200.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827200.1

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GATGGCAGAA GCGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCCGCAGAGC AAAGAACAAG CTCAAAGGAG TCTAGCCCCA TCCCCTCCCC CACGTCCGAC 
CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCTCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACCGCT GCCTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC 
GTGTTCAAGC TAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_023251678.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023107446.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

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CloneID	OFb73568
Clone ID Related Accession (Same CDS sequence)	XM_023251678.1 , XM_023251677.1
Accession Version	XM_023251678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT 
AGCCCTGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGAGGTT CAGCATTTTT 
GGCAAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107446.1
CDS	146..7240
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRMAEAVDTSEMVNGAAEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSIFGKKK

Target ORF information:

RefSeq Version	XM_023251678.1
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023251678.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT 
AGCCCTGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGAGGTT CAGCATTTTT 
GGCAAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_023251677.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023107445.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

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CloneID	OFb73568
Clone ID Related Accession (Same CDS sequence)	XM_023251678.1 , XM_023251677.1
Accession Version	XM_023251677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT 
AGCCCTGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGAGGTT CAGCATTTTT 
GGCAAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107445.1
CDS	255..7349
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRMAEAVDTSEMVNGAAEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSIFGKKK

Target ORF information:

RefSeq Version	XM_023251677.1
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023251677.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
GTCAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCCGC AAGGCTCTTT 
GAGCGGTCCC GAATCAAGGC TCTGGCAGAT GAGCGTGAAG CTGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTCGCCCGG GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAAG TTCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGTATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACC 
AGCTGGAGGG ACGGCATGGC CTTCAATGCC CTGATACACA AACACCGGCC TGACCTGATA 
GATTTCGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAATGC GTTTAATCTG 
GCAGAGCAGC ACCTTGGTCT CACTAAACTG TTGGATCCGG AAGATATCAG TGTGGACCAC 
CCTGATGAGA AGTCCATTAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGATC TTCTGGAGTG GATTGAACAA 
ACCATCATTA TATTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG 
CTTCAGGCTT TTAACACTTA CCGCACTGTG GAGAAACCAC CCAAGTTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GGGCCAACAA TCAGAAGGTC 
TACATGCCCC GGGAGGGCAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATCAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT CGATCGCAAG GCAGCTATGA GGGAAACCTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGTTTTGACC TTCCTGCTGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATCGCCGCAT ACGAGGAACG TGTGCAGGCG 
GTGGTAGCAG TGGCCAGGGA GCTTGAGGCG GAGAACTACC ATGACATCAA GCGCATCACA 
GCAAGGAAAG ACAATGTCAT CCGGCTCTGG GAGTACCTGC TGGAACTGCT CAGGGCCCGG 
AGGCAGCGTC TTGAGATGAA CCTGGGGCTG CAAAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTGTCTC AAGACTATGG CAAGCACTTG 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCGGAGCGAG TGAGAGGCGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAC 
CAAGAACTCT GCCAGCTGGC AGCCGAACGC CGGGCTCGAC TGGAAGAGTC CCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAGGGCTGGA TACGAGAGAA GGAGAAGATC 
CTGTCCTCCG ACGATTATGG GAAGGACCTG ACCAGCGTCA TGCGCTTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCATT TCGAGCAGGC CATCAAAGAA 
GGTGAAGACA TGATCGCGGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GCGGATCCTT 
TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CGCTGCTACA CCAGTTCCAG GCAGACGCCG ACGACATTGA CGCGTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAAT GACGTGGGCC ATGACGAGTA CTCCACGCAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCA GAGGAGATCG CCAACTATAG GCCCACCATC 
GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCCGGC TGTCAGGCAT CGAGGAACGG TACAAGGAGG TGGCAGAGCT GACGCGGCTG 
AGGAAGCAGG CACTGCAGGA CACCCTGGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAAAAA 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TTGAGCCTGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGAC AGCTCATGCA CAGTGGCCAC 
CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGTCAGTTC 
CGAGAGCTGG TCGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC 
CACCTTGAGT GCAATGAAAC CAAATCCTGG ATTCGAGAAA AGACAAAGGT CATCGAGTCC 
ACTCAGGACC TGGGCAACGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACTGGC 
ATGGAGCGGG ACCTGGTGGC CATCGAGGCA AAGCTGAGCG ACCTGCAGAA AGAGGCGGAG 
AAGCTCGAGT CTGAGCACCC CGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACAACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAT CTGGACGACT TCCAGTCCTG GCTCTCCCGG 
ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TGACTGAGGC AGAGAAGCTC 
CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACCGGG TGGAACGAGC TGCACAAAAT GTGGGAGAAC 
AGGCAGAATC TCCTGTCCCA GTCACATGCC TACCAGCAGT TCCTTAGGGA CACGAAGCAG 
GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTCGTGGAG ACAGGCCGGA GGCTGGTGAG CGATGGAAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGGAAGAAT 
CGCGAGGCAG CCAGCGAGCT TCTGATGCGT TTGAAGGACA ACCGGGATCT TCAGAAATTC 
CTGCAAGATT GTCAGGAGTT ATCTCTTTGG ATCAATGAAA AGATGCTTAC TGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAACATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAC 
ATGTGGGAAG TCCTCGAGTC CACCACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTTAAA 
AAGCAACAGA TGCTGGAGAA CCAGATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG 
AGCCAGGCCC AGGCCCTGAG TCAGGAGGGG AAGAGCACTG ACGAGGTGGA CAGCAAGCGC 
CTCACTGTGC AGACCAAGTT CATGGAACTG CTGGAGCCCT TGAATGAAAG AAAGCATAAT 
CTGCTGGCCT CTAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATTTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAACGCC 
GAAGCCATTC GACGGAGGCT CGCTGACCTC AAGGAGCTAT GGGGTCTCCT CATTGAGGAG 
ACCGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT 
GCTGCTGAGG CTGAGGCATG GATGAGTGAG CAAGAGCTGT ACATGATGTC TGAGGAGAAG 
GCCAAGGATG AGCAGAGCGC CGTCTCCATG TTGAAGAAGC ACCAGATTCT GGAACAAGCC 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT 
GACAGCCATC CGGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC 
GCGGGTCTGA AAGACCTGGC CGAAGAGCGG AGGGGCAAGC TGGACGAGAG GCACAGGCTC 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCGC ATGAGTTGGG GCAGGACTAC GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCAGAGACAC AGGGAACATT GGACAGGAGC GTGTGGACAC AGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGTCAT TCGGATGCCG CCACCATCGC CGAGTGGAAG 
GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTGGAGCTCA TCGACACGAG AACACAGATT 
CTCGCCGCCT CTTATGAACT GCACAAGTTT TACCATGATG CCAAGGAGAT CTTTGGGCGC 
ATACAGGACA AACACAAGAA ACTCCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC CACGTTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTC 
AGGCAGCTGC AGGAGGATGC GGCCCGTCTA CAGGCAGCCT ACGCGGGTGA CAAGGCTGAC 
GACATCCAGA AGCGGGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCCTGT 
GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTA 
CGAGACCTCA TGCTGTGGAT GGAAGACGTC ATCCGGCAGA TTGAAGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCATC AAGGTATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGCTT TACAACTTGC ATTGAACTTG GGAAATCCCT ACTGGCGAGG 
AAGCACTATG CATCTGAGGA GATCAAGGAA AAGCTATTGC AGTTGACGGA AAAGAGAAAA 
GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC 
CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGACAAGA GCCATACCTA 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAT GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCTTTTGAAA AGTCTGCAGC AACCTGGGAC GAGAGGTTCT CTGCCCTGGA AAGGCTGACA 
ACGTTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCCTCTCCTG AGCCGAGCAC GAAGGCTTCT GAGGAGATGG AGTCCCAGCA GCAGTGGGAT 
ACCTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGA CTGAACAGGG ATCTCCACGG 
ATGGCAGAAG CGGTGGACAC AAGCGAAATG GTCAACGGCG CCGCAGAGCA AAGAACAAGC 
TCAAAGGAGT CTAGCCCCAT CCCCTCCCCC ACGTCCGACC GCAAAGCCAA GACTGCCCTC 
CCGGCCCAGA GTGCCGCCAC CTTGCCGGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TGAATCGGAA GCACGAGTGG GAGGCTCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACCGCTG CCTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC 
ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT 
AGCCCTGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGAGGTT CAGCATTTTT 
GGCAAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_023251679.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023107447.1 Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

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CloneID	OFb73569
Clone ID Related Accession (Same CDS sequence)	XM_023251679.1
Accession Version	XM_023251679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GATGGCAGAA GCGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCCGCAGAGC AAAGAACAAG CTCAAAGGAG TCTAGCCCCA TCCCCTCCCC CACGTCCGAC 
CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCTCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACCGCT GCCTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC 
GTGTTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG 
GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC TAGCCCTGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG 
GAGAAGAGGT TCAGCATTTT TGGCAAAAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023107447.1
CDS	444..7499
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERILYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPTIDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHNMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRRRLADLKELWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKASEEMESQQQWDTSKGEQVSQNGLPTEQGSPRMAEAVDTSEMVNGAAEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSIFGKKK

Target ORF information:

RefSeq Version	XM_023251679.1
Organism	Felis catus(domestic cat)
Definition	Felis catus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023251679.1

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCT GGGCCGCTGT CCCCAGCTTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTACAAGA TGAGCGTGAA 
GCTGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTCGCCCG GGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAA 
GTTCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGTAT CCACTGTCTG 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC 
AACATTCACA ATTTCACCAC CAGCTGGAGG GACGGCATGG CCTTCAATGC CCTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTCGAC AAACTAAAGA AATCCAATGC ACACTACAAT 
CTGCAGAATG CGTTTAATCT GGCAGAGCAG CACCTTGGTC TCACTAAACT GTTGGATCCG 
GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCCATTA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAT 
CTTCTGGAGT GGATTGAACA AACCATCATT ATATTGAACA ATCGCAAATT TGCCAATTCT 
CTGGTTGGGG TTCAACAACA GCTTCAGGCT TTTAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG 
AGGGCCAACA ATCAGAAGGT CTACATGCCC CGGGAGGGCA AGCTCATCTC TGACATTAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG 
CTCATCAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGGGAAACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGTTTTGAC 
CTTCCTGCTG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATCGCCGCA 
TACGAGGAAC GTGTGCAGGC GGTGGTAGCA GTGGCCAGGG AGCTTGAGGC GGAGAACTAC 
CATGACATCA AGCGCATCAC AGCAAGGAAA GACAATGTCA TCCGGCTCTG GGAGTACCTG 
CTGGAACTGC TCAGGGCCCG GAGGCAGCGT CTTGAGATGA ACCTGGGGCT GCAAAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTGTCT 
CAAGACTATG GCAAGCACTT GCTTGGTGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCGGAGCGA GTGAGAGGCG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCTC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA CCAAGAACTC TGCCAGCTGG CAGCCGAACG CCGGGCTCGA 
CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAGGGCTGG 
ATACGAGAGA AGGAGAAGAT CCTGTCCTCC GACGATTATG GGAAGGACCT GACCAGCGTC 
ATGCGCTTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAT 
TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCGG AGGAGCACTT TGGGTCAGAG 
AAGATCCGAG AGCGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTAC ACCAGTTCCA GGCAGACGCC 
GACGACATTG ACGCGTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAA TGACGTGGGC 
CATGACGAGT ACTCCACGCA GTCTCTGGTC AAGAAACACA AGGACGTGGC AGAGGAGATC 
GCCAACTATA GGCCCACCAT CGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCCGG CTGTCAGGCA TCGAGGAACG GTACAAGGAG 
GTGGCAGAGC TGACGCGGCT GAGGAAGCAG GCACTGCAGG ACACCCTGGC CTTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAAAA ACTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTTGAGCCTG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGA 
CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGTCAGTT CCGAGAGCTG GTCGACAGGA AGAAGGACGC TCTTCTCTCT 
GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAATGAAA CCAAATCCTG GATTCGAGAA 
AAGACAAAGG TCATCGAGTC CACTCAGGAC CTGGGCAACG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTGGTGG CCATCGAGGC AAAGCTGAGC 
GACCTGCAGA AAGAGGCGGA GAAGCTCGAG TCTGAGCACC CCGACCAGGC TCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAAAAC 
CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA TCTGGACGAC 
TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC 
CTGACTGAGG CAGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC 
GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACCGG GTGGAACGAG 
CTGCACAAAA TGTGGGAGAA CAGGCAGAAT CTCCTGTCCC AGTCACATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTCGTGGA GACAGGCCGG 
AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT 
GATGACAGAC ATAGGAAGAA TCGCGAGGCA GCCAGCGAGC TTCTGATGCG TTTGAAGGAC 
AACCGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGT TATCTCTTTG GATCAATGAA 
AAGATGCTTA CTGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTAAAGGAA 
AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTCGAGT CCACCACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC 
AGCGTCAACA TCCTGCTTAA AAAGCAACAG ATGCTGGAGA ACCAGATGGA GGTGCGGAAG 
AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG GAAGAGCACT 
GACGAGGTGG ACAGCAAGCG CCTCACTGTG CAGACCAAGT TCATGGAACT GCTGGAGCCC 
TTGAATGAAA GAAAGCATAA TCTGCTGGCC TCTAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC 
GACAGCAGCA GCCTCAACGC CGAAGCCATT CGACGGAGGC TCGCTGACCT CAAGGAGCTA 
TGGGGTCTCC TCATTGAGGA GACCGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTCGA TGCTGCTGAG GCTGAGGCAT GGATGAGTGA GCAAGAGCTG 
TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGCG CCGTCTCCAT GTTGAAGAAG 
CACCAGATTC TGGAACAAGC CGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCTAAG 
ACCAGCCGGG CCCTGGTGGC TGACAGCCAT CCGGAAAGTG AGCGCATCAG CATGCGGCAG 
TCCAAAGTGG ACAAGCTGTA CGCGGGTCTG AAAGACCTGG CCGAAGAGCG GAGGGGCAAG 
CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCG CATGAGTTGG GGCAGGACTA CGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCAGAGACA CAGGGAACAT TGGACAGGAG 
CGTGTGGACA CAGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGTCA TTCGGATGCC 
GCCACCATCG CCGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATCGACACGA GAACACAGAT TCTCGCCGCC TCTTATGAAC TGCACAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTCCCCGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CCACGTTTGA GCATGACATC 
CAGGCTCTGG GCACTCAGGT CAGGCAGCTG CAGGAGGATG CGGCCCGTCT ACAGGCAGCC 
TACGCGGGTG ACAAGGCTGA CGACATCCAG AAGCGGGAGA ATGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT ACGAGACCTC ATGCTGTGGA TGGAAGACGT CATCCGGCAG 
ATTGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAT 
CAAGGTATCA AAGCTGAAAT CGATGCTCGT AATGACAGCT TTACAACTTG CATTGAACTT 
GGGAAATCCC TACTGGCGAG GAAGCACTAT GCATCTGAGG AGATCAAGGA AAAGCTATTG 
CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG 
CTCGGACAAG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA TGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAGTCTGCAG CAACCTGGGA CGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC AACGTTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCCTCTCCT GAGCCGAGCA CGAAGGCTTC TGAGGAGATG 
GAGTCCCAGC AGCAGTGGGA TACCTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG 
ACTGAACAGG GATCTCCACG GATGGCAGAA GCGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCCGCAGAGC AAAGAACAAG CTCAAAGGAG TCTAGCCCCA TCCCCTCCCC CACGTCCGAC 
CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCCGCCA CCTTGCCGGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTGAATCGGA AGCACGAGTG GGAGGCTCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACCGCT GCCTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC 
GTGTTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG 
GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC TAGCCCTGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG 
GAGAAGAGGT TCAGCATTTT TGGCAAAAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTBN1 cDNA ORF clone, Felis catus(Domestic cat)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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