HECTD1 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD1
Entrez Gene ID	101086980
Full Name	HECT domain E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023255630.1	XP_023111398.1	E3 ubiquitin-protein ligase HECTD1 isoform X5
XM_023255625.1	XP_023111393.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_023255628.1	XP_023111396.1	E3 ubiquitin-protein ligase HECTD1 isoform X3
XM_023255627.1	XP_023111395.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_011283365.2	XP_011281667.1	E3 ubiquitin-protein ligase HECTD1
XM_023255626.1	XP_023111394.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_023255629.1	XP_023111397.1	E3 ubiquitin-protein ligase HECTD1 isoform X4

Pathways from BioSystems
Gene Ontology

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb28240	XM_011283365.2 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77182	XM_023255626.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77182	XM_023255625.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77183	XM_023255627.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77184	XM_023255628.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77185	XM_023255629.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb77186	XM_023255630.1 Latest version!	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD1 ( XM_011283365.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011281667.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

Price & Availability↑

CloneID	OFb28240
Clone ID Related Accession (Same CDS sequence)	XM_011283365.2
Accession Version	XM_011283365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011283365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACAGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGATTA CATTTTTCTT CTTGTAGCCC TGGTTTCCTG 
AGATTCGTGA GGGTTTTGTG TGGCATGTCT TCAGATGAAA GGAAAGCATT CCTGCAGTTT 
ACCACTGGTT GCTCAACTCT TCCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACA 
GTTGTTCGCA AGGTCGATGC TACTGATGCA AGCTATCCAT CAGTAAATAC ATGTGTGCAT 
TATCTCAAGT TACCGGAATA TTCTTCAGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACCATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011281667.1
CDS	366..7895
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLHFSSCSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_011283365.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011283365.2

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACAGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGATTA CATTTTTCTT CTTGTAGCCC TGGTTTCCTG 
AGATTCGTGA GGGTTTTGTG TGGCATGTCT TCAGATGAAA GGAAAGCATT CCTGCAGTTT 
ACCACTGGTT GCTCAACTCT TCCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACA 
GTTGTTCGCA AGGTCGATGC TACTGATGCA AGCTATCCAT CAGTAAATAC ATGTGTGCAT 
TATCTCAAGT TACCGGAATA TTCTTCAGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACCATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255625.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111393.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

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CloneID	OFb77182
Clone ID Related Accession (Same CDS sequence)	XM_023255625.1 , XM_023255626.1
Accession Version	XM_023255625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA 
CAGATGGAAG CATTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGATTCG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG 
TTTACCACTG GTTGCTCAAC TCTTCCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACAGTTGTTC GCAAGGTCGA TGCTACTGAT GCAAGCTATC CATCAGTAAA TACATGTGTG 
CATTATCTCA AGTTACCGGA ATATTCTTCA GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACCATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111393.1
CDS	355..8187
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255625.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255625.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA 
CAGATGGAAG CATTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGATTCG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG 
TTTACCACTG GTTGCTCAAC TCTTCCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACAGTTGTTC GCAAGGTCGA TGCTACTGAT GCAAGCTATC CATCAGTAAA TACATGTGTG 
CATTATCTCA AGTTACCGGA ATATTCTTCA GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACCATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255626.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111394.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

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CloneID	OFb77182
Clone ID Related Accession (Same CDS sequence)	XM_023255625.1 , XM_023255626.1
Accession Version	XM_023255626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA 
CAGATGGAAG CATTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGATTCG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG 
TTTACCACTG GTTGCTCAAC TCTTCCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACAGTTGTTC GCAAGGTCGA TGCTACTGAT GCAAGCTATC CATCAGTAAA TACATGTGTG 
CATTATCTCA AGTTACCGGA ATATTCTTCA GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACCATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111394.1
CDS	464..8296
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255626.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255626.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGATTAA ACCAGGGGGC CATTTCTACC CTTCAGAGTA GTGATATTCT TAATTTGACG 
AAAGAACAAC CTCAGGCCAA AGCCGGTAAT GGTCAGAATT CTTGTGGAGT GGAAGATGTC 
CTTCAGCTGC TGCGTATTCT CTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAA 
GAAGAAGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATCACAACAA AAATTTTGCA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCGTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACCGTGGA AAGAACAAGG ACCACCAGTA GTGTAAGGCG AGATGATCCT 
GGGGAGTTTC GAGTGGGTCG TCTCAAGCAT GAAAGAGTAA AAGTTCCACG TGGTGAATCT 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAATTTT TAGGAGAAGA AGGAACTGGC TTGGGCCCTA CATTAGAATT TTATGCTCTG 
GTGGCAGCAG AATTCCAGAG AACTGATTTG GGAGCGTGGC TTTGTGATGA TAACTTTCCA 
GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTATGTG 
CAGAGATCAT GTGGGCTGTT CACGGCACCA TTTCCACAAG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TCTTTCATTT CCTTGGGATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAGCCTTTC TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAATTAAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCTGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAGTT TATTCTAGAT CCCCCCAAAC CAAAACCCCC AGCTTGGTTT 
AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACCGAGC CCGATTTTTA 
AAAGAAATTA AAGACCTTGC TGTCAAGAGG CGTCAGATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACGAA ATTACAGGAA CTAGTGCTAA AGAATCCATC AGGGTCTGGT 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATTCAGAAA 
CAGATGGAAG CATTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGATTCG TGAGGGTTTT GTGTGGCATG TCTTCAGATG AAAGGAAAGC ATTCCTGCAG 
TTTACCACTG GTTGCTCAAC TCTTCCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACAGTTGTTC GCAAGGTCGA TGCTACTGAT GCAAGCTATC CATCAGTAAA TACATGTGTG 
CATTATCTCA AGTTACCGGA ATATTCTTCA GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACCATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255627.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111395.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

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CloneID	OFb77183
Clone ID Related Accession (Same CDS sequence)	XM_023255627.1
Accession Version	XM_023255627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGA TTCTGAGACG TCCATCCCTA CAGCGTCGAG CTGGCTCCCG CTCTGATGTA 
ACACACCATG CTGTCACGTC GCAGCTGCCA CAAGTCCCTG CTGGAGCAGG GAGCCGACCT 
ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGAG GACGTCGGAG AACATGGGAT 
GATGATTATG TGCTTAAGCG GCAGTTTTCT GCTTTGGTTC CTGCTTTTGA TCCTAGACCT 
GGTCGGACTA ATGTCCAACA AACAACTGAT CTAGAGATTC CGCCCCCAGG AACACCTCAT 
TCAGAGCTCT TAGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA 
GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACT 
ATTTTTTACT ATGTACAAAA ATTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT 
AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT 
GACAAAGAGA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC 
CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC 
GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA 
AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA 
TTAAACCAGG GGGCCATTTC TACCCTTCAG AGTAGTGATA TTCTTAATTT GACGAAAGAA 
CAACCTCAGG CCAAAGCCGG TAATGGTCAG AATTCTTGTG GAGTGGAAGA TGTCCTTCAG 
CTGCTGCGTA TTCTCTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAAGAAGAA 
GGTGATGAGC AGCCCCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA 
ACAAAAATTT TGCAGCAGAT TGAGGAACCA TTGGCATTGG CAAGTGGGGC TCTGCCAGAC 
TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT 
TATTTCACAT GTACAGCATT TGGTGCGTCA AGAGCAATAG TGTGGTTACA AAACCGACGT 
GAAGCCACCG TGGAAAGAAC AAGGACCACC AGTAGTGTAA GGCGAGATGA TCCTGGGGAG 
TTTCGAGTGG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGTGA ATCTCTGATG 
GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA 
TTTTTAGGAG AAGAAGGAAC TGGCTTGGGC CCTACATTAG AATTTTATGC TCTGGTGGCA 
GCAGAATTCC AGAGAACTGA TTTGGGAGCG TGGCTTTGTG ATGATAACTT TCCAGATGAT 
GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA 
TCATGTGGGC TGTTCACGGC ACCATTTCCA CAAGATAGTG ATGAGCTTGA AAGGATCACA 
AAACTCTTTC ATTTCCTTGG GATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG 
GACTTACCTA TTTCTAAGCC TTTCTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT 
ATGAGTAAAT TAATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG 
TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCTG TAGGAAGCTT TGAAGAGGAT 
TCAAAATCAG AGTTTATTCT AGATCCCCCC AAACCAAAAC CCCCAGCTTG GTTTAATGGA 
ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACC GAGCCCGATT TTTAAAAGAA 
ATTAAAGACC TTGCTGTCAA GAGGCGTCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT 
GAGAAGAACA CGAAATTACA GGAACTAGTG CTAAAGAATC CATCAGGGTC TGGTCCTCCA 
CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT 
TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA 
GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAGATG 
GAAGCATTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC 
CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT 
ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGA 
TTCGTGAGGG TTTTGTGTGG CATGTCTTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC 
ACTGGTTGCT CAACTCTTCC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACAGTT 
GTTCGCAAGG TCGATGCTAC TGATGCAAGC TATCCATCAG TAAATACATG TGTGCATTAT 
CTCAAGTTAC CGGAATATTC TTCAGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACC 
ATGGAGAAAG GCTTTCATCT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111395.1
CDS	355..8181
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255627.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255627.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGA TTCTGAGACG TCCATCCCTA CAGCGTCGAG CTGGCTCCCG CTCTGATGTA 
ACACACCATG CTGTCACGTC GCAGCTGCCA CAAGTCCCTG CTGGAGCAGG GAGCCGACCT 
ATTGGGGAGC AGGAAGAAGA AGAGTATGAA ACTAAAGGAG GACGTCGGAG AACATGGGAT 
GATGATTATG TGCTTAAGCG GCAGTTTTCT GCTTTGGTTC CTGCTTTTGA TCCTAGACCT 
GGTCGGACTA ATGTCCAACA AACAACTGAT CTAGAGATTC CGCCCCCAGG AACACCTCAT 
TCAGAGCTCT TAGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTGAAA 
GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACT 
ATTTTTTACT ATGTACAAAA ATTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT 
AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT 
GACAAAGAGA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC 
CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC 
GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA 
AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA 
TTAAACCAGG GGGCCATTTC TACCCTTCAG AGTAGTGATA TTCTTAATTT GACGAAAGAA 
CAACCTCAGG CCAAAGCCGG TAATGGTCAG AATTCTTGTG GAGTGGAAGA TGTCCTTCAG 
CTGCTGCGTA TTCTCTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAAGAAGAA 
GGTGATGAGC AGCCCCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA 
ACAAAAATTT TGCAGCAGAT TGAGGAACCA TTGGCATTGG CAAGTGGGGC TCTGCCAGAC 
TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT 
TATTTCACAT GTACAGCATT TGGTGCGTCA AGAGCAATAG TGTGGTTACA AAACCGACGT 
GAAGCCACCG TGGAAAGAAC AAGGACCACC AGTAGTGTAA GGCGAGATGA TCCTGGGGAG 
TTTCGAGTGG GTCGTCTCAA GCATGAAAGA GTAAAAGTTC CACGTGGTGA ATCTCTGATG 
GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAA 
TTTTTAGGAG AAGAAGGAAC TGGCTTGGGC CCTACATTAG AATTTTATGC TCTGGTGGCA 
GCAGAATTCC AGAGAACTGA TTTGGGAGCG TGGCTTTGTG ATGATAACTT TCCAGATGAT 
GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA TGTGCAGAGA 
TCATGTGGGC TGTTCACGGC ACCATTTCCA CAAGATAGTG ATGAGCTTGA AAGGATCACA 
AAACTCTTTC ATTTCCTTGG GATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG 
GACTTACCTA TTTCTAAGCC TTTCTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT 
ATGAGTAAAT TAATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG 
TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCTG TAGGAAGCTT TGAAGAGGAT 
TCAAAATCAG AGTTTATTCT AGATCCCCCC AAACCAAAAC CCCCAGCTTG GTTTAATGGA 
ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACC GAGCCCGATT TTTAAAAGAA 
ATTAAAGACC TTGCTGTCAA GAGGCGTCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT 
GAGAAGAACA CGAAATTACA GGAACTAGTG CTAAAGAATC CATCAGGGTC TGGTCCTCCA 
CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT 
TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA 
GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATTCA GAAACAGATG 
GAAGCATTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC 
CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAGGAT 
ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGA 
TTCGTGAGGG TTTTGTGTGG CATGTCTTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC 
ACTGGTTGCT CAACTCTTCC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACAGTT 
GTTCGCAAGG TCGATGCTAC TGATGCAAGC TATCCATCAG TAAATACATG TGTGCATTAT 
CTCAAGTTAC CGGAATATTC TTCAGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACC 
ATGGAGAAAG GCTTTCATCT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255628.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111396.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

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CloneID	OFb77184
Clone ID Related Accession (Same CDS sequence)	XM_023255628.1
Accession Version	XM_023255628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC 
TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT 
GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA 
TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT 
GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCATC CAGTGCAAGC 
ACCAGCACCT TAACCGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT 
AGTTCTGTTC GCACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT 
TCTCCTGATG TTAGTTCAGT GTCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGACCC 
CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTAAGTTCTT TGTTGGCTGC TGGGGCCCCT 
ATGAGCTCTA GTGCAAGTGT ACCTAATCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT 
TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC 
CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT 
TCTCCTCTCA TGGGTGCTCA GAGTTTCCCC AATTTGACGA CTCCTGGTAC CACATCAACA 
GTGACAATGT CAACATCCAG TGTGACTAGC AGCAGCAATG TAGCCACAGC AACAACAGTT 
TTATCAGTTG GTCAGTCCTT AAGTAATACT TTAACCACCA GTCTCACGTC AACCTCCAGT 
GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCGTTATATG ATTTCCTGGA TAGCTGCCGT 
GCCAGTACTC TATTAGCTGA GCTAGATGAT GATGAAGACT TGCCCGAGCC AGATGAGGAA 
GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG 
AGACGTCCAT CCCTACAGCG TCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC 
ACGTCGCAGC TGCCACAAGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA 
GAAGAAGAGT ATGAAACTAA AGGAGGACGT CGGAGAACAT GGGATGATGA TTATGTGCTT 
AAGCGGCAGT TTTCTGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG GACTAATGTC 
CAACAAACAA CTGATCTAGA GATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTAGAA 
GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA 
ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACTATTTT TTACTATGTA 
CAAAAATTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT 
TGGGAGCCAA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGACAA AGAGAAGGAA 
AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA 
TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC 
CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC 
ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCC 
ATTTCTACCC TTCAGAGTAG TGATATTCTT AATTTGACGA AAGAACAACC TCAGGCCAAA 
GCCGGTAATG GTCAGAATTC TTGTGGAGTG GAAGATGTCC TTCAGCTGCT GCGTATTCTC 
TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAAG AAGAAGGTGA TGAGCAGCCC 
CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG 
CAGATTGAGG AACCATTGGC ATTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA 
ACCAGCAAGT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA 
GCATTTGGTG CGTCAAGAGC AATAGTGTGG TTACAAAACC GACGTGAAGC CACCGTGGAA 
AGAACAAGGA CCACCAGTAG TGTAAGGCGA GATGATCCTG GGGAGTTTCG AGTGGGTCGT 
CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGTGAATCTC TGATGGAATG GGCTGAGAAT 
GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA 
GGAACTGGCT TGGGCCCTAC ATTAGAATTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA 
ACTGATTTGG GAGCGTGGCT TTGTGATGAT AACTTTCCAG ATGATGAATC TCGTCATGTT 
GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGGCTGTTC 
ACGGCACCAT TTCCACAAGA TAGTGATGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC 
CTTGGGATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT 
AAGCCTTTCT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAATTAATT 
TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA 
GAAGAAGGTC ATGATTCACT CTCTGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAGTTT 
ATTCTAGATC CCCCCAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA 
GACTTTGAAC TAGTAAACCC ACACCGAGCC CGATTTTTAA AAGAAATTAA AGACCTTGCT 
GTCAAGAGGC GTCAGATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACGAAA 
TTACAGGAAC TAGTGCTAAA GAATCCATCA GGGTCTGGTC CTCCACTTAG CATAGAGGAT 
TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT 
CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT 
TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC ATTTAGAGAT 
GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA 
ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT 
GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGATTCGT GAGGGTTTTG 
TGTGGCATGT CTTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG TTGCTCAACT 
CTTCCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CAGTTGTTCG CAAGGTCGAT 
GCTACTGATG CAAGCTATCC ATCAGTAAAT ACATGTGTGC ATTATCTCAA GTTACCGGAA 
TATTCTTCAG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACCATGGA GAAAGGCTTT 
CATCTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111396.1
CDS	355..8046
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255628.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255628.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC 
TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT 
GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA 
TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT 
GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCATC CAGTGCAAGC 
ACCAGCACCT TAACCGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT 
AGTTCTGTTC GCACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT 
TCTCCTGATG TTAGTTCAGT GTCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGACCC 
CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTAAGTTCTT TGTTGGCTGC TGGGGCCCCT 
ATGAGCTCTA GTGCAAGTGT ACCTAATCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT 
TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC 
CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT 
TCTCCTCTCA TGGGTGCTCA GAGTTTCCCC AATTTGACGA CTCCTGGTAC CACATCAACA 
GTGACAATGT CAACATCCAG TGTGACTAGC AGCAGCAATG TAGCCACAGC AACAACAGTT 
TTATCAGTTG GTCAGTCCTT AAGTAATACT TTAACCACCA GTCTCACGTC AACCTCCAGT 
GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCGTTATATG ATTTCCTGGA TAGCTGCCGT 
GCCAGTACTC TATTAGCTGA GCTAGATGAT GATGAAGACT TGCCCGAGCC AGATGAGGAA 
GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG 
AGACGTCCAT CCCTACAGCG TCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC 
ACGTCGCAGC TGCCACAAGT CCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA 
GAAGAAGAGT ATGAAACTAA AGGAGGACGT CGGAGAACAT GGGATGATGA TTATGTGCTT 
AAGCGGCAGT TTTCTGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG GACTAATGTC 
CAACAAACAA CTGATCTAGA GATTCCGCCC CCAGGAACAC CTCATTCAGA GCTCTTAGAA 
GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA 
ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACTATTTT TTACTATGTA 
CAAAAATTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT 
TGGGAGCCAA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGACAA AGAGAAGGAA 
AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA 
TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC 
CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC 
ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCC 
ATTTCTACCC TTCAGAGTAG TGATATTCTT AATTTGACGA AAGAACAACC TCAGGCCAAA 
GCCGGTAATG GTCAGAATTC TTGTGGAGTG GAAGATGTCC TTCAGCTGCT GCGTATTCTC 
TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAAG AAGAAGGTGA TGAGCAGCCC 
CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG 
CAGATTGAGG AACCATTGGC ATTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA 
ACCAGCAAGT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA 
GCATTTGGTG CGTCAAGAGC AATAGTGTGG TTACAAAACC GACGTGAAGC CACCGTGGAA 
AGAACAAGGA CCACCAGTAG TGTAAGGCGA GATGATCCTG GGGAGTTTCG AGTGGGTCGT 
CTCAAGCATG AAAGAGTAAA AGTTCCACGT GGTGAATCTC TGATGGAATG GGCTGAGAAT 
GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAATTTTT AGGAGAAGAA 
GGAACTGGCT TGGGCCCTAC ATTAGAATTT TATGCTCTGG TGGCAGCAGA ATTCCAGAGA 
ACTGATTTGG GAGCGTGGCT TTGTGATGAT AACTTTCCAG ATGATGAATC TCGTCATGTT 
GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTATGTGC AGAGATCATG TGGGCTGTTC 
ACGGCACCAT TTCCACAAGA TAGTGATGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC 
CTTGGGATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT 
AAGCCTTTCT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAATTAATT 
TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA 
GAAGAAGGTC ATGATTCACT CTCTGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAGTTT 
ATTCTAGATC CCCCCAAACC AAAACCCCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA 
GACTTTGAAC TAGTAAACCC ACACCGAGCC CGATTTTTAA AAGAAATTAA AGACCTTGCT 
GTCAAGAGGC GTCAGATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACGAAA 
TTACAGGAAC TAGTGCTAAA GAATCCATCA GGGTCTGGTC CTCCACTTAG CATAGAGGAT 
TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT 
CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT 
TTGATGTTTG ACTTTTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC ATTTAGAGAT 
GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAAGTCCAA 
ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AGGATATTAT TAATTATACT 
GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGATTCGT GAGGGTTTTG 
TGTGGCATGT CTTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG TTGCTCAACT 
CTTCCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CAGTTGTTCG CAAGGTCGAT 
GCTACTGATG CAAGCTATCC ATCAGTAAAT ACATGTGTGC ATTATCTCAA GTTACCGGAA 
TATTCTTCAG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACCATGGA GAAAGGCTTT 
CATCTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255629.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111397.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

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CloneID	OFb77185
Clone ID Related Accession (Same CDS sequence)	XM_023255629.1
Accession Version	XM_023255629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT 
GGATTAAACC AGGGGGCCAT TTCTACCCTT CAGAGTAGTG ATATTCTTAA TTTGACGAAA 
GAACAACCTC AGGCCAAAGC CGGTAATGGT CAGAATTCTT GTGGAGTGGA AGATGTCCTT 
CAGCTGCTGC GTATTCTCTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAAGAA 
GAAGGTGATG AGCAGCCCCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC 
ACAACAAAAA TTTTGCAGCA GATTGAGGAA CCATTGGCAT TGGCAAGTGG GGCTCTGCCA 
GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCATTTGA AACTAGACAG 
CTTTATTTCA CATGTACAGC ATTTGGTGCG TCAAGAGCAA TAGTGTGGTT ACAAAACCGA 
CGTGAAGCCA CCGTGGAAAG AACAAGGACC ACCAGTAGTG TAAGGCGAGA TGATCCTGGG 
GAGTTTCGAG TGGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG TGAATCTCTG 
ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT 
GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGCCCTACAT TAGAATTTTA TGCTCTGGTG 
GCAGCAGAAT TCCAGAGAAC TGATTTGGGA GCGTGGCTTT GTGATGATAA CTTTCCAGAT 
GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG 
AGATCATGTG GGCTGTTCAC GGCACCATTT CCACAAGATA GTGATGAGCT TGAAAGGATC 
ACAAAACTCT TTCATTTCCT TGGGATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT 
GTGGACTTAC CTATTTCTAA GCCTTTCTTT AAACTTATGT GTATGGGTGA CATTAAAAGC 
AATATGAGTA AATTAATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT 
CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CTGTAGGAAG CTTTGAAGAG 
GATTCAAAAT CAGAGTTTAT TCTAGATCCC CCCAAACCAA AACCCCCAGC TTGGTTTAAT 
GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACCGAGCCCG ATTTTTAAAA 
GAAATTAAAG ACCTTGCTGT CAAGAGGCGT CAGATTTTAA GCAACAAAGG TCTTTCTGAA 
GATGAGAAGA ACACGAAATT ACAGGAACTA GTGCTAAAGA ATCCATCAGG GTCTGGTCCT 
CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT 
GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT 
GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAG 
ATGGAAGCAT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC 
AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG 
GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGCCC TGGTTTCCTG 
AGATTCGTGA GGGTTTTGTG TGGCATGTCT TCAGATGAAA GGAAAGCATT CCTGCAGTTT 
ACCACTGGTT GCTCAACTCT TCCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACA 
GTTGTTCGCA AGGTCGATGC TACTGATGCA AGCTATCCAT CAGTAAATAC ATGTGTGCAT 
TATCTCAAGT TACCGGAATA TTCTTCAGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACCATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111397.1
CDS	355..8004
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255629.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255629.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCATCAT CCAGTGCAAG CACCAGCACC TTAACCGCGG AAACGGGAAG TGAAAATGCT 
GAAAGAAAGT TAGGCCCTGA TAGTTCTGTT CGCACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TGTCTGAGTT AACTAATAAA 
GAAGCAGCTT CACAACGACC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTAAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAATCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTC ATGGGTGCTC AGAGTTTCCC CAATTTGACG 
ACTCCTGGTA CCACATCAAC AGTGACAATG TCAACATCCA GTGTGACTAG CAGCAGCAAT 
GTAGCCACAG CAACAACAGT TTTATCAGTT GGTCAGTCCT TAAGTAATAC TTTAACCACC 
AGTCTCACGT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCGTTATAT 
GATTTCCTGG ATAGCTGCCG TGCCAGTACT CTATTAGCTG AGCTAGATGA TGATGAAGAC 
TTGCCCGAGC CAGATGAGGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGTCCA TCCCTACAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACAC ACCATGCTGT CACGTCGCAG CTGCCACAAG TCCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TATGAAACTA AAGGAGGACG TCGGAGAACA 
TGGGATGATG ATTATGTGCT TAAGCGGCAG TTTTCTGCTT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GGACTAATGT CCAACAAACA ACTGATCTAG AGATTCCGCC CCCAGGAACA 
CCTCATTCAG AGCTCTTAGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA 
TCAACTATTT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGACA AAGAGAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT 
GGATTAAACC AGGGGGCCAT TTCTACCCTT CAGAGTAGTG ATATTCTTAA TTTGACGAAA 
GAACAACCTC AGGCCAAAGC CGGTAATGGT CAGAATTCTT GTGGAGTGGA AGATGTCCTT 
CAGCTGCTGC GTATTCTCTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAAGAA 
GAAGGTGATG AGCAGCCCCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC 
ACAACAAAAA TTTTGCAGCA GATTGAGGAA CCATTGGCAT TGGCAAGTGG GGCTCTGCCA 
GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCATTTGA AACTAGACAG 
CTTTATTTCA CATGTACAGC ATTTGGTGCG TCAAGAGCAA TAGTGTGGTT ACAAAACCGA 
CGTGAAGCCA CCGTGGAAAG AACAAGGACC ACCAGTAGTG TAAGGCGAGA TGATCCTGGG 
GAGTTTCGAG TGGGTCGTCT CAAGCATGAA AGAGTAAAAG TTCCACGTGG TGAATCTCTG 
ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT 
GAATTTTTAG GAGAAGAAGG AACTGGCTTG GGCCCTACAT TAGAATTTTA TGCTCTGGTG 
GCAGCAGAAT TCCAGAGAAC TGATTTGGGA GCGTGGCTTT GTGATGATAA CTTTCCAGAT 
GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTATGTGCAG 
AGATCATGTG GGCTGTTCAC GGCACCATTT CCACAAGATA GTGATGAGCT TGAAAGGATC 
ACAAAACTCT TTCATTTCCT TGGGATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT 
GTGGACTTAC CTATTTCTAA GCCTTTCTTT AAACTTATGT GTATGGGTGA CATTAAAAGC 
AATATGAGTA AATTAATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT 
CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CTGTAGGAAG CTTTGAAGAG 
GATTCAAAAT CAGAGTTTAT TCTAGATCCC CCCAAACCAA AACCCCCAGC TTGGTTTAAT 
GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACCGAGCCCG ATTTTTAAAA 
GAAATTAAAG ACCTTGCTGT CAAGAGGCGT CAGATTTTAA GCAACAAAGG TCTTTCTGAA 
GATGAGAAGA ACACGAAATT ACAGGAACTA GTGCTAAAGA ATCCATCAGG GTCTGGTCCT 
CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT 
GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT 
GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT TCAGAAACAG 
ATGGAAGCAT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC 
AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAG 
GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGCCC TGGTTTCCTG 
AGATTCGTGA GGGTTTTGTG TGGCATGTCT TCAGATGAAA GGAAAGCATT CCTGCAGTTT 
ACCACTGGTT GCTCAACTCT TCCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACA 
GTTGTTCGCA AGGTCGATGC TACTGATGCA AGCTATCCAT CAGTAAATAC ATGTGTGCAT 
TATCTCAAGT TACCGGAATA TTCTTCAGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACCATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_023255630.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023111398.1 Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

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CloneID	OFb77186
Clone ID Related Accession (Same CDS sequence)	XM_023255630.1
Accession Version	XM_023255630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGCTTCTCCT CTCATGGGTG CTCAGAGTTT CCCCAATTTG 
ACGACTCCTG GTACCACATC AACAGTGACA ATGTCAACAT CCAGTGTGAC TAGCAGCAGC 
AATGTAGCCA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CCTTAAGTAA TACTTTAACC 
ACCAGTCTCA CGTCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCGTTA 
TATGATTTCC TGGATAGCTG CCGTGCCAGT ACTCTATTAG CTGAGCTAGA TGATGATGAA 
GACTTGCCCG AGCCAGATGA GGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA 
GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCCCTAC AGCGTCGAGC TGGCTCCCGC 
TCTGATGTAA CACACCATGC TGTCACGTCG CAGCTGCCAC AAGTCCCTGC TGGAGCAGGG 
AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTATGAAA CTAAAGGAGG ACGTCGGAGA 
ACATGGGATG ATGATTATGT GCTTAAGCGG CAGTTTTCTG CTTTGGTTCC TGCTTTTGAT 
CCTAGACCTG GTCGGACTAA TGTCCAACAA ACAACTGATC TAGAGATTCC GCCCCCAGGA 
ACACCTCATT CAGAGCTCTT AGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC 
ACTTTGAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC 
AGATCAACTA TTTTTTACTA TGTACAAAAA TTGCTTCAGT TGTCCTGTAA TGGCAATGTG 
AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATCATGTA CAGAGAAATG 
AAGGATTCTG ACAAAGAGAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG 
GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG 
AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG 
AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA 
AAGTCTGGAT TAAACCAGGG GGCCATTTCT ACCCTTCAGA GTAGTGATAT TCTTAATTTG 
ACGAAAGAAC AACCTCAGGC CAAAGCCGGT AATGGTCAGA ATTCTTGTGG AGTGGAAGAT 
GTCCTTCAGC TGCTGCGTAT TCTCTATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC 
CAAGAAGAAG GTGATGAGCA GCCCCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA 
AAAATCACAA CAAAAATTTT GCAGCAGATT GAGGAACCAT TGGCATTGGC AAGTGGGGCT 
CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC ATTTGAAACT 
AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCGTCAA GAGCAATAGT GTGGTTACAA 
AACCGACGTG AAGCCACCGT GGAAAGAACA AGGACCACCA GTAGTGTAAG GCGAGATGAT 
CCTGGGGAGT TTCGAGTGGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGTGAA 
TCTCTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT 
GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGCC CTACATTAGA ATTTTATGCT 
CTGGTGGCAG CAGAATTCCA GAGAACTGAT TTGGGAGCGT GGCTTTGTGA TGATAACTTT 
CCAGATGATG AATCTCGTCA TGTTGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT 
GTGCAGAGAT CATGTGGGCT GTTCACGGCA CCATTTCCAC AAGATAGTGA TGAGCTTGAA 
AGGATCACAA AACTCTTTCA TTTCCTTGGG ATTTTCTTGG CCAAATGCAT TCAAGACAAT 
AGACTTGTGG ACTTACCTAT TTCTAAGCCT TTCTTTAAAC TTATGTGTAT GGGTGACATT 
AAAAGCAATA TGAGTAAATT AATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT 
GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCTGT AGGAAGCTTT 
GAAGAGGATT CAAAATCAGA GTTTATTCTA GATCCCCCCA AACCAAAACC CCCAGCTTGG 
TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACCG AGCCCGATTT 
TTAAAAGAAA TTAAAGACCT TGCTGTCAAG AGGCGTCAGA TTTTAAGCAA CAAAGGTCTT 
TCTGAAGATG AGAAGAACAC GAAATTACAG GAACTAGTGC TAAAGAATCC ATCAGGGTCT 
GGTCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA 
ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGGG AAGATGAGAT GATAACAATG 
GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG 
AAACAGATGG AAGCATTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT 
TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA 
GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGT 
TTCCTGAGAT TCGTGAGGGT TTTGTGTGGC ATGTCTTCAG ATGAAAGGAA AGCATTCCTG 
CAGTTTACCA CTGGTTGCTC AACTCTTCCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG 
CTCACAGTTG TTCGCAAGGT CGATGCTACT GATGCAAGCT ATCCATCAGT AAATACATGT 
GTGCATTATC TCAAGTTACC GGAATATTCT TCAGAGGAGA TCATGAGAGA GCGCCTGCTA 
GCTGCTACCA TGGAGAAAGG CTTTCATCTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023111398.1
CDS	355..7350
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTAADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTVLVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSVDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNASPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEEGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAVKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_023255630.1
Organism	Felis catus(domestic cat)
Definition	Felis catus HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023255630.1

ORF Insert Sequence:

ATGGCAGATG TGGATCCTGA TACATTGTTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AGCTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT 
TTTCTTGATG AAAGTGCTCC GGACAATGTG TTAGAGGTGA CAGCCCGTGC CATCACATAC 
TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTAGT TGTGGTCGAA CTTAATAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTGTT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTCGAG 
GCTGGTGGCT TAAATTGTGT GCTTACCTTC ATTCGTGACA GCGGACACTT GGTTCATAAA 
GATACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAACCTCAA 
GATTCTTCTT TAGAAATTTG TGTGGAATCT CTGTCTAGTT TATTAAAACA TGAGGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CAGACCGGTT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCGAAGCAT GGATTAACTG AGGAGCTGTT ATCTCGCATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCTT GTAAACCAGG TCGCAGCACT 
ACAGGAGCGC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTCA CACACGATCT CCTGAGGTCA 
GAACTTCCGG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTTCGCA GATTGGATAG CTCCGGGGAG 
CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT 
GATGTTAATA GGGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAACCCAG ATCTGAGAGA TGAAGATGGG 
AAGACTCCAT TAGATAAAGC TCGGGAAAGG GGCCACAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AATAAAGATG AAGAAGAATG TAATGAGCCA AAAGGAGATC CGGAAATGGC ACCTATATAC 
TTGAAAAGAT TATTGCCAGT GTTTGCACAG ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAGGCAA GTCTTGCTCT CATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT GGAAATCACT 
GCAACCGTCC TTGATCAGGA GGATGATGAT GATGGTCACT TGCTGGCATT GCAGATCATA 
AGGGATTTGG TGGATAAAGG TGGTGATATT TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCAACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA GGGTAAACCG 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGGGACT GCTTATATAT ATGGAGCGAT 
GCAGCAGCCT TGGAATTATC TAACGGCAGT AATGGATGGT TCAGATTTAT CTTGGACGGG 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCAGATAG TTCAGAAAGC 
AGAAGTGAAT TCTTAGAGAA ATTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCT ACTAAACTCA CTGTAGGGAA TTGGTCACTA 
ACATGTTTAA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCAACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGTT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAATTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TATGATGACC ACTTCAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACGCTCAGA 
AACATAGCAA CTCAATTAGA GTCATCTTGG GAACTTCACA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGCAGTA GTGGGTTGGT GCAAGCCCTT 
CTTACTGTTT TAAACAATAG TGTGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ACGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGGAA GTTAATAGCT GTACTTGAAT CTATTGAACG TCTCCCTCTC 
CATTTGTATG ATACACCTGG ATCTACGTAT AACCTGCAGA TACTTACAAG AAGATTAAGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATAG ACAGGACTGG CAGGATGTTG 
AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA 
AAGCAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGGACAAATG CCAAAACTGC ATATGAATGG GTGAATCCAG CTGCTTATGG ACTTGTAGTA 
GTAACATCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG CCACAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCAAC CTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGGAAG 
AATGCCAGTG GACAAACCCA CTACCTGTCA CTATCTGGAT TCGAACTTTA TGGCACTGTA 
AACGGAGTAT GTGAAGACCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA 
AGACAAAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG GGCACGTGTG 
ATCAGAGGCC TTGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGTACT 
GTCACAGGAG AACTGCATAA TGCTTCTCCT CTCATGGGTG CTCAGAGTTT CCCCAATTTG 
ACGACTCCTG GTACCACATC AACAGTGACA ATGTCAACAT CCAGTGTGAC TAGCAGCAGC 
AATGTAGCCA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CCTTAAGTAA TACTTTAACC 
ACCAGTCTCA CGTCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCGTTA 
TATGATTTCC TGGATAGCTG CCGTGCCAGT ACTCTATTAG CTGAGCTAGA TGATGATGAA 
GACTTGCCCG AGCCAGATGA GGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA 
GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCCCTAC AGCGTCGAGC TGGCTCCCGC 
TCTGATGTAA CACACCATGC TGTCACGTCG CAGCTGCCAC AAGTCCCTGC TGGAGCAGGG 
AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTATGAAA CTAAAGGAGG ACGTCGGAGA 
ACATGGGATG ATGATTATGT GCTTAAGCGG CAGTTTTCTG CTTTGGTTCC TGCTTTTGAT 
CCTAGACCTG GTCGGACTAA TGTCCAACAA ACAACTGATC TAGAGATTCC GCCCCCAGGA 
ACACCTCATT CAGAGCTCTT AGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC 
ACTTTGAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC 
AGATCAACTA TTTTTTACTA TGTACAAAAA TTGCTTCAGT TGTCCTGTAA TGGCAATGTG 
AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATCATGTA CAGAGAAATG 
AAGGATTCTG ACAAAGAGAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG 
GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG 
AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG 
AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA 
AAGTCTGGAT TAAACCAGGG GGCCATTTCT ACCCTTCAGA GTAGTGATAT TCTTAATTTG 
ACGAAAGAAC AACCTCAGGC CAAAGCCGGT AATGGTCAGA ATTCTTGTGG AGTGGAAGAT 
GTCCTTCAGC TGCTGCGTAT TCTCTATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC 
CAAGAAGAAG GTGATGAGCA GCCCCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA 
AAAATCACAA CAAAAATTTT GCAGCAGATT GAGGAACCAT TGGCATTGGC AAGTGGGGCT 
CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC ATTTGAAACT 
AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCGTCAA GAGCAATAGT GTGGTTACAA 
AACCGACGTG AAGCCACCGT GGAAAGAACA AGGACCACCA GTAGTGTAAG GCGAGATGAT 
CCTGGGGAGT TTCGAGTGGG TCGTCTCAAG CATGAAAGAG TAAAAGTTCC ACGTGGTGAA 
TCTCTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT 
GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGCTTGGGCC CTACATTAGA ATTTTATGCT 
CTGGTGGCAG CAGAATTCCA GAGAACTGAT TTGGGAGCGT GGCTTTGTGA TGATAACTTT 
CCAGATGATG AATCTCGTCA TGTTGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAT 
GTGCAGAGAT CATGTGGGCT GTTCACGGCA CCATTTCCAC AAGATAGTGA TGAGCTTGAA 
AGGATCACAA AACTCTTTCA TTTCCTTGGG ATTTTCTTGG CCAAATGCAT TCAAGACAAT 
AGACTTGTGG ACTTACCTAT TTCTAAGCCT TTCTTTAAAC TTATGTGTAT GGGTGACATT 
AAAAGCAATA TGAGTAAATT AATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT 
GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCTGT AGGAAGCTTT 
GAAGAGGATT CAAAATCAGA GTTTATTCTA GATCCCCCCA AACCAAAACC CCCAGCTTGG 
TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACCG AGCCCGATTT 
TTAAAAGAAA TTAAAGACCT TGCTGTCAAG AGGCGTCAGA TTTTAAGCAA CAAAGGTCTT 
TCTGAAGATG AGAAGAACAC GAAATTACAG GAACTAGTGC TAAAGAATCC ATCAGGGTCT 
GGTCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA 
ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGGG AAGATGAGAT GATAACAATG 
GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATTCAG 
AAACAGATGG AAGCATTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT 
TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA 
GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGT 
TTCCTGAGAT TCGTGAGGGT TTTGTGTGGC ATGTCTTCAG ATGAAAGGAA AGCATTCCTG 
CAGTTTACCA CTGGTTGCTC AACTCTTCCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG 
CTCACAGTTG TTCGCAAGGT CGATGCTACT GATGCAAGCT ATCCATCAGT AAATACATGT 
GTGCATTATC TCAAGTTACC GGAATATTCT TCAGAGGAGA TCATGAGAGA GCGCCTGCTA 
GCTGCTACCA TGGAGAAAGG CTTTCATCTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECTD1 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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