TJP1 gene cDNA ORF clone, Felis catus(Domestic cat)

mRNA	Protein	Name
XM_023254996.1	XP_023110764.1	tight junction protein ZO-1 isoform X8
XM_011282728.2	XP_011281030.2	tight junction protein ZO-1 isoform X3
XM_019831954.2	XP_019687513.1	tight junction protein ZO-1 isoform X6
XM_011282728.3	XP_011281030.2	tight junction protein ZO-1 isoform X3
XM_019831952.1	XP_019687511.1	tight junction protein ZO-1 isoform X4
XM_019831951.2	XP_019687510.1	tight junction protein ZO-1 isoform X2
XM_019831950.1	XP_019687509.1	tight junction protein ZO-1 isoform X1
XM_019831950.2	XP_019687509.1	tight junction protein ZO-1 isoform X1
XM_019831951.1	XP_019687510.1	tight junction protein ZO-1 isoform X2
XM_023254994.1	XP_023110762.1	tight junction protein ZO-1 isoform X5
XM_019831953.2	XP_019687512.1	tight junction protein ZO-1 isoform X4
XM_019831954.1	XP_019687513.1	tight junction protein ZO-1 isoform X6
XM_023254995.1	XP_023110763.1	tight junction protein ZO-1 isoform X7
XM_019831953.1	XP_019687512.1	tight junction protein ZO-1 isoform X5
XM_019831955.1	XP_019687514.1	tight junction protein ZO-1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb43525	XM_019831950.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43526	XM_019831951.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43527	XM_011282728.2 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43528	XM_019831952.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43529	XM_019831953.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43530	XM_019831954.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43531	XM_019831955.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76454	XM_019831950.2 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76455	XM_019831951.2 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76456	XM_011282728.3 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76457	XM_019831953.2 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76458	XM_023254994.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76459	XM_019831954.2 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76460	XM_023254995.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76461	XM_023254996.1 Latest version!	Felis catus tight junction protein 1 (TJP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

TJP1 ( XM_019831950.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687509.1 Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb43525
Clone ID Related Accession (Same CDS sequence)	XM_019831950.1
Accession Version	XM_019831950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687509.1
CDS	16..5886
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831950.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831950.1

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831951.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687510.1 Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OFb43526
Clone ID Related Accession (Same CDS sequence)	XM_019831951.1
Accession Version	XM_019831951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTACCTT GTTGGAGCAA ACTGCGTTTC TGTCCTGATA 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687510.1
CDS	16..5847
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831951.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831951.1

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTACCTT GTTGGAGCAA ACTGCGTTTC TGTCCTGATA 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_011282728.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_011281030.2 Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OFb43527
Clone ID Related Accession (Same CDS sequence)	XM_011282728.2
Accession Version	XM_011282728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011282728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011281030.2
CDS	16..5826
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_011282728.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011282728.2

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831952.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687511.1 Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb43528
Clone ID Related Accession (Same CDS sequence)	XM_019831952.1
Accession Version	XM_019831952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687511.1
CDS	16..5766
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831952.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831952.1

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831953.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687512.1 Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OFb43529
Clone ID Related Accession (Same CDS sequence)	XM_019831953.1
Accession Version	XM_019831953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687512.1
CDS	16..5646
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831953.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831953.1

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831954.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687513.1 Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OFb43530
Clone ID Related Accession (Same CDS sequence)	XM_019831954.1
Accession Version	XM_019831954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687513.1
CDS	16..5586
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831954.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831954.1

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTGGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTTTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GGCAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTACGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831955.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687514.1 Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OFb43531
Clone ID Related Accession (Same CDS sequence)	XM_019831955.1
Accession Version	XM_019831955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	tight junction protein ZO-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACA TGGAGAAGGT AACGCCATCC TCTGAAAATC CGAATGGTGC TACTTCTAGC 
GTCAGCCAAG GAAAACCTTC TTTAAGACGA ATAAAAGGGA GACTCCACAG AAGCAAAAGC 
CTTGATAGCA TCGATTTCTG TGAACTCACT AACACAGCAA TGGAGGAAAC AGCTATATGG 
GAACAACACA CAGTGACGCT TCACAGGGCT CCTGGATTTG GATTTGGAAT TGCAATATCT 
GGTGGAAGAG ATAATCCTCA TTTTCAGAGT GGGGAAACGT CAATAGTAAT CTCAGATGTT 
CTGAAAGGGG GACCTGCTGA AGGACAGCTA CAGGAAAATG ACCGGGTTGC AATGGTTAAT 
GGAGTTTCAA TGGATAATGT TGAACATGCT TTTGCTGTTC AGCAACTAAG AAAAAGTGGG 
AAGAATGCAA AAATTACCAT TAGGAGGAAG AAGAAAGTTC AGATACCAGT TAGTCGTCCT 
GATCCTGAAC CTGTATCTGA AAAAGAAGAT GATAGTTATG ATGAGGAGGT CCATGATCCG 
AGAAGCAGCC GTGGAGGTCT GGTCAGTAGA AGGAGTGAGA AGAGTTGGGC AAGGGATAGA 
AGTGCAAGCA GAGAGAGGAG TTTGTCCCCA CGGTCTGATA GGCGATCTGT GGCCTCCAGT 
CAGCCTGCCA AACCCACCAA AGTCACATTG GTGAAATCCC GGAAAAATGA AGAATATGGT 
CTTCGATTGG CCAGTCATAT ATTTGTGAAG GAAATTTCAC AAGATAGTTT GGCAGCAAGA 
GATGGCAATA TTCAAGAAGG CGATGTTGTA TTAAAGATAA ATGGTACCGT GACAGAAAAT 
ATGTCACTGA CAGATGCAAA GACATTGATA GAAAGGTCTA AAGGCAAGTT AAAAATGGTA 
GTTCAGAGAG ATGAACGAGC TACCCTGTTG AATGTCCCTG ATCTTTCTGA CAGTATCCAT 
TCTGCTAATG CCTCTGAGAG AGATGACATT TCAGAAATTC AGTCACTGGC ATCAGATCAT 
TCTGGTCGAT CACATGACAG ACCTCCCCGC CACAGCCGGT CACGATCACC TGACCAACGG 
TCAGAGCCTT CTGATCATTC CAGACACTCT CCACAGCAGC CCAGCAGTGG CAGTCTCCGG 
AGCAGAGAGG AAGAGAGAAT TTCTAAACCT GGGGCTGTCT CAACTCCTGT AAAGCATGCG 
GATGATCATA CACCTAAAAC AGAAGAAGTT GTAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTATACGCT CAAGTTGGGC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGCGT CTTGCCTAAT TCAACTCATG AAGATGGGAT TCTGCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTCTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGTGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAAAT TCTCAGGGTA AACAATGTAG ATTTCACAAA CATCATAAGA 
GAAGAAGCCG TTCTTTTCCT GCTCGACCTC CCTAAAGGAG AAGAAGTGAC CATACTGGCT 
CAGAAAAAGA AAGATGTTTA TCGTCGCATC GTTGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAG TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TAGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA AGAAGTAGAA CGAGGCATCA TCCCCAATAA GAACAGGGCT 
GAGCAGTTAG CCAGTGTACA ATACACACTT CCAAAAACAG CAGGAGGAGA CCGTGCTGAC 
TTTTGGAGAT TCCGGGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAGAG TAGAGAGGAT 
TTGTCAGCTC AGCCAGTTCA AACAAAGTTT CCAGCCTATG AAAGAGTTGT TCTTCGAGAA 
GCTGGATTTC TGAGACCTGT AACTATCTTT GGACCCATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACGA TAAAGCAAAT AATAGATCAG 
GACAAACATG CTTTATTAGA TGTAACCCCA AATGCAGTTG ATCGTCTGAA TTACGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCT GATTCTAAGC AAGGTGTGAA AACTATGAGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TGTATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCATTGAA AGAAGCCATT CAGCAACAGC AAAACCAGTT GGTATGGGTT 
TCTGAAGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTATTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAACCTCTG CCGTTAATCA TAATGTAACT TTAACTAATG TAAGACTGGA GGGGCCCACC 
CCAGCTCCTT CCACATCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAG CCGTCATTGT CGTCGCATGT AGATCCAGCA 
AAGGTGTATA GAAAAGATCC CTATCCTGAG GAAATGATGA GACAGAATCA TGTTTTAAAA 
CAGCCACCCG TTGGTCACCC AGGGCAGAGG CCAGACAAAG AGCCTAATCT GAGCTATGAA 
TCCCAACCCC CATATGTAGA GAAACAAGCC AGTAGAGACC TTGAACAGCC TGTGTACAGA 
TACGACTCCT CTGGCTACCC AGACCAGTTT TCTCGAAACT ATGACCATCG TCTACGCTAT 
GAAGAGCGCA TTCCTACATA TGAAGAGCAG TGGTCGTATT ACGATGACAA ACAGCCCTAC 
CAGCCTCGGC CTTCTTTTGA TAATCAGCAT CCTCGGGACC TTGACTCCAG ACAGCATCCT 
GAAGAGTCCT CAGAACGAGG CTATTACCCA CGTTTTGAAG AGCCAGCCCC TCTACCCTAT 
GACAGCAGAC CACGTTATGA CCAACCACCT AGAACCTCCA CCCTACGACA TGAAGAGCAG 
CCAACTCCTG GATATGACAT GCATAACAGA TACAGACCAG AAGCACAGTC CTATTCTTCA 
GCAGGCCCGA AGGCATCTGA ACCCAAGCAG TATTTTGACC AATACCCACG AAGTTATGAG 
CAAGTACCAT CACAAGGATT TACCTCTAAA GCAGGCCACT ATGAGCCTCT CCATGGTGCT 
GCAGTCATCC CTCCTCTAGT ACCCACATCT CAACATAAGC CAGAAGTTCT GCCTTCAAAT 
ACCAAACCAC TGCCTCCACC CCCATCTCTG ACTGAAGAGG AAGAAGATCC AGCAATGAAA 
CCCCAGTCTG TGCTCACTAG AGTTAAAATG TTTGAAAACA AAAGATCTGC ATCGATGGAG 
AATAAGAAGG ATGAAAACCA CACTGCTGGT TTTAAGCCTC CAGAGGTAGC ATCTAAACCT 
CCAGGTGCTG CCATCGTTGG TCCTAAAGCC ACTCCTCAGA ATCAATTCAG TGAACATGAC 
AAAACACTGT ACAGGATCCC AGAACCTCAG AAACCTCAAA TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTACGA TCCCGAGGAG GATGAAGAAT ATTATCGAAA GCAACTCTCC 
TACTTTGACC GAAGAAGTTT TGAAAACAAG CCTTCTACAC ACATTCCTAC TGGCCATCTC 
TCAGAGCCTG CCAAGCCAGT TCATTCTCAG AATCAACCAA ACTTTTCTAG TTACTCTTCA 
AAGGGAAAGT CTCCTGAAGC TGACACTGCG GATAGATCAT TTGGTGAGAA ACGCTATGAG 
CCGATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCCC AGTATGCCCA GACCAGCTCC 
TCTCTCGCCC TCCACACGCA TGCCAAGGGG GCACATGGTG AAGGTAATTC GGTGTCATTG 
GATTTCCAGA ATTCTTTAGT GTCTAAACCA GATCCACCTC CGTCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCGAACCG AGAAGATACT GTTCAGTCTA CTTTCTATCC CCAGAAAAGT 
TTCCCGGATA AAGCTCCAGT TAATGGAGGT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TATAATCGAT TCACACCAAA ACCATACACA AGTTCTGCTC GGCCATTTGA GCGCAAGTTT 
GAAAGTCCTA AATTCAACCA CAATCTTTTG CCAAGTGAAA CTGCACATAA ACCTGATTTG 
TCATCAAAAG CCCCTACTTC ACCAAAAACT CTTGTGAAAG CGCACAGTGT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCCATCCATG CAGATAAGCC CAAATACCAA 
ATGAATAATG TCAGCACTGT GCCTAAAGCT GTTCCTGTGA GCCCTTCAGC TGTGGAAGAA 
GATGAAGATG AAGATGGTCA CACCGTGGTG GCCACAGCCC GAGGCGTGTT TAACAGCAAT 
GGTGGGGTGT TGAGTTCCAT AGAAACCGGC GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAGGGAA TTGAGCAGGA AATCTATTTC AAGGTCTGCC GAGACAACAG CATCCTCCCA 
CCTTTAGATA AGGAGAAAGG CGAGACACTG CTGAGCCCCT TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TACCTTGTTG GAGCAAACTG CGTTTCTGTC 
CTGATAGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687514.1
CDS	23..5440
Translation	MKDMEKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831955.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831955.1

ORF Insert Sequence:

ATGAAGGACA TGGAGAAGGT AACGCCATCC TCTGAAAATC CGAATGGTGC TACTTCTAGC 
GTCAGCCAAG GAAAACCTTC TTTAAGACGA ATAAAAGGGA GACTCCACAG AAGCAAAAGC 
CTTGATAGCA TCGATTTCTG TGAACTCACT AACACAGCAA TGGAGGAAAC AGCTATATGG 
GAACAACACA CAGTGACGCT TCACAGGGCT CCTGGATTTG GATTTGGAAT TGCAATATCT 
GGTGGAAGAG ATAATCCTCA TTTTCAGAGT GGGGAAACGT CAATAGTAAT CTCAGATGTT 
CTGAAAGGGG GACCTGCTGA AGGACAGCTA CAGGAAAATG ACCGGGTTGC AATGGTTAAT 
GGAGTTTCAA TGGATAATGT TGAACATGCT TTTGCTGTTC AGCAACTAAG AAAAAGTGGG 
AAGAATGCAA AAATTACCAT TAGGAGGAAG AAGAAAGTTC AGATACCAGT TAGTCGTCCT 
GATCCTGAAC CTGTATCTGA AAAAGAAGAT GATAGTTATG ATGAGGAGGT CCATGATCCG 
AGAAGCAGCC GTGGAGGTCT GGTCAGTAGA AGGAGTGAGA AGAGTTGGGC AAGGGATAGA 
AGTGCAAGCA GAGAGAGGAG TTTGTCCCCA CGGTCTGATA GGCGATCTGT GGCCTCCAGT 
CAGCCTGCCA AACCCACCAA AGTCACATTG GTGAAATCCC GGAAAAATGA AGAATATGGT 
CTTCGATTGG CCAGTCATAT ATTTGTGAAG GAAATTTCAC AAGATAGTTT GGCAGCAAGA 
GATGGCAATA TTCAAGAAGG CGATGTTGTA TTAAAGATAA ATGGTACCGT GACAGAAAAT 
ATGTCACTGA CAGATGCAAA GACATTGATA GAAAGGTCTA AAGGCAAGTT AAAAATGGTA 
GTTCAGAGAG ATGAACGAGC TACCCTGTTG AATGTCCCTG ATCTTTCTGA CAGTATCCAT 
TCTGCTAATG CCTCTGAGAG AGATGACATT TCAGAAATTC AGTCACTGGC ATCAGATCAT 
TCTGGTCGAT CACATGACAG ACCTCCCCGC CACAGCCGGT CACGATCACC TGACCAACGG 
TCAGAGCCTT CTGATCATTC CAGACACTCT CCACAGCAGC CCAGCAGTGG CAGTCTCCGG 
AGCAGAGAGG AAGAGAGAAT TTCTAAACCT GGGGCTGTCT CAACTCCTGT AAAGCATGCG 
GATGATCATA CACCTAAAAC AGAAGAAGTT GTAGTTGAAA GAAATGAGAA ACAAGCACCC 
ACTCTTCCAG AACCAAAGCC TGTATACGCT CAAGTTGGGC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGCGT CTTGCCTAAT TCAACTCATG AAGATGGGAT TCTGCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTCTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGTGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAAGAGG GTGATCAAAT TCTCAGGGTA AACAATGTAG ATTTCACAAA CATCATAAGA 
GAAGAAGCCG TTCTTTTCCT GCTCGACCTC CCTAAAGGAG AAGAAGTGAC CATACTGGCT 
CAGAAAAAGA AAGATGTTTA TCGTCGCATC GTTGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAG TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TAGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA AGAAGTAGAA CGAGGCATCA TCCCCAATAA GAACAGGGCT 
GAGCAGTTAG CCAGTGTACA ATACACACTT CCAAAAACAG CAGGAGGAGA CCGTGCTGAC 
TTTTGGAGAT TCCGGGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAGAG TAGAGAGGAT 
TTGTCAGCTC AGCCAGTTCA AACAAAGTTT CCAGCCTATG AAAGAGTTGT TCTTCGAGAA 
GCTGGATTTC TGAGACCTGT AACTATCTTT GGACCCATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTTCATACGA TAAAGCAAAT AATAGATCAG 
GACAAACATG CTTTATTAGA TGTAACCCCA AATGCAGTTG ATCGTCTGAA TTACGCCCAG 
TGGTATCCAA TTGTAGTATT CCTTAACCCT GATTCTAAGC AAGGTGTGAA AACTATGAGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TGTATGAACG GTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCATTGAA AGAAGCCATT CAGCAACAGC AAAACCAGTT GGTATGGGTT 
TCTGAAGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTATTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAC CCCAGCATCA 
TCAACCTCTG CCGTTAATCA TAATGTAACT TTAACTAATG TAAGACTGGA GGGGCCCACC 
CCAGCTCCTT CCACATCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAG CCGTCATTGT CGTCGCATGT AGATCCAGCA 
AAGGTGTATA GAAAAGATCC CTATCCTGAG GAAATGATGA GACAGAATCA TGTTTTAAAA 
CAGCCACCCG TTGGTCACCC AGGGCAGAGG CCAGACAAAG AGCCTAATCT GAGCTATGAA 
TCCCAACCCC CATATGTAGA GAAACAAGCC AGTAGAGACC TTGAACAGCC TGTGTACAGA 
TACGACTCCT CTGGCTACCC AGACCAGTTT TCTCGAAACT ATGACCATCG TCTACGCTAT 
GAAGAGCGCA TTCCTACATA TGAAGAGCAG TGGTCGTATT ACGATGACAA ACAGCCCTAC 
CAGCCTCGGC CTTCTTTTGA TAATCAGCAT CCTCGGGACC TTGACTCCAG ACAGCATCCT 
GAAGAGTCCT CAGAACGAGG CTATTACCCA CGTTTTGAAG AGCCAGCCCC TCTACCCTAT 
GACAGCAGAC CACGTTATGA CCAACCACCT AGAACCTCCA CCCTACGACA TGAAGAGCAG 
CCAACTCCTG GATATGACAT GCATAACAGA TACAGACCAG AAGCACAGTC CTATTCTTCA 
GCAGGCCCGA AGGCATCTGA ACCCAAGCAG TATTTTGACC AATACCCACG AAGTTATGAG 
CAAGTACCAT CACAAGGATT TACCTCTAAA GCAGGCCACT ATGAGCCTCT CCATGGTGCT 
GCAGTCATCC CTCCTCTAGT ACCCACATCT CAACATAAGC CAGAAGTTCT GCCTTCAAAT 
ACCAAACCAC TGCCTCCACC CCCATCTCTG ACTGAAGAGG AAGAAGATCC AGCAATGAAA 
CCCCAGTCTG TGCTCACTAG AGTTAAAATG TTTGAAAACA AAAGATCTGC ATCGATGGAG 
AATAAGAAGG ATGAAAACCA CACTGCTGGT TTTAAGCCTC CAGAGGTAGC ATCTAAACCT 
CCAGGTGCTG CCATCGTTGG TCCTAAAGCC ACTCCTCAGA ATCAATTCAG TGAACATGAC 
AAAACACTGT ACAGGATCCC AGAACCTCAG AAACCTCAAA TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTACGA TCCCGAGGAG GATGAAGAAT ATTATCGAAA GCAACTCTCC 
TACTTTGACC GAAGAAGTTT TGAAAACAAG CCTTCTACAC ACATTCCTAC TGGCCATCTC 
TCAGAGCCTG CCAAGCCAGT TCATTCTCAG AATCAACCAA ACTTTTCTAG TTACTCTTCA 
AAGGGAAAGT CTCCTGAAGC TGACACTGCG GATAGATCAT TTGGTGAGAA ACGCTATGAG 
CCGATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCCC AGTATGCCCA GACCAGCTCC 
TCTCTCGCCC TCCACACGCA TGCCAAGGGG GCACATGGTG AAGGTAATTC GGTGTCATTG 
GATTTCCAGA ATTCTTTAGT GTCTAAACCA GATCCACCTC CGTCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCGAACCG AGAAGATACT GTTCAGTCTA CTTTCTATCC CCAGAAAAGT 
TTCCCGGATA AAGCTCCAGT TAATGGAGGT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TATAATCGAT TCACACCAAA ACCATACACA AGTTCTGCTC GGCCATTTGA GCGCAAGTTT 
GAAAGTCCTA AATTCAACCA CAATCTTTTG CCAAGTGAAA CTGCACATAA ACCTGATTTG 
TCATCAAAAG CCCCTACTTC ACCAAAAACT CTTGTGAAAG CGCACAGTGT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCCATCCATG CAGATAAGCC CAAATACCAA 
ATGAATAATG TCAGCACTGT GCCTAAAGCT GTTCCTGTGA GCCCTTCAGC TGTGGAAGAA 
GATGAAGATG AAGATGGTCA CACCGTGGTG GCCACAGCCC GAGGCGTGTT TAACAGCAAT 
GGTGGGGTGT TGAGTTCCAT AGAAACCGGC GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAGGGAA TTGAGCAGGA AATCTATTTC AAGGTCTGCC GAGACAACAG CATCCTCCCA 
CCTTTAGATA AGGAGAAAGG CGAGACACTG CTGAGCCCCT TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TACCTTGTTG GAGCAAACTG CGTTTCTGTC 
CTGATAGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831950.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687509.1 Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

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CloneID	OFb76454
Clone ID Related Accession (Same CDS sequence)	XM_019831950.2
Accession Version	XM_019831950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019831950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687509.1
CDS	16..5886
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831950.2
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831950.2

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831951.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687510.1 Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OFb76455
Clone ID Related Accession (Same CDS sequence)	XM_019831951.2
Accession Version	XM_019831951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019831951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTACCTT GTTGGAGCAA ACTGCGTTTC TGTCCTGATA 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687510.1
CDS	16..5847
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831951.2
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831951.2

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTACCTT GTTGGAGCAA ACTGCGTTTC TGTCCTGATA 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_011282728.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_011281030.2 Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OFb76456
Clone ID Related Accession (Same CDS sequence)	XM_011282728.3
Accession Version	XM_011282728.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_011282728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011281030.2
CDS	16..5826
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_011282728.3
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011282728.3

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGTC 
CCTTACCTTT CGCCTGAAAC AACCCCAGCA TCATCAACCT CTGCCGTTAA TCATAATGTA 
ACTTTAACTA ATGTAAGACT GGAGGGGCCC ACCCCAGCTC CTTCCACATC TTACTCACCA 
CAAGCTGATT CTTTAAGAAC ACCAAGTACT GAGGCAGCTC ACATAATGCT AAGAGATCAA 
GAGCCGTCAT TGTCGTCGCA TGTAGATCCA GCAAAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831953.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687512.1 Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OFb76457
Clone ID Related Accession (Same CDS sequence)	XM_019831953.2
Accession Version	XM_019831953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019831953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687512.1
CDS	16..5646
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831953.2
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831953.2

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_023254994.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110762.1 Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OFb76458
Clone ID Related Accession (Same CDS sequence)	XM_023254994.1
Accession Version	XM_023254994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGAAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCGAA TGGTGCTACT TCTAGCGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATAA AAGGGAGACT CCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTAATCTCA GATGTTCTGA AAGGGGGACC TGCTGAAGGA 
CAGCTACAGG AAAATGACCG GGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGAAAA AGTGGGAAGA ATGCAAAAAT TACCATTAGG 
AGGAAGAAGA AAGTTCAGAT ACCAGTTAGT CGTCCTGATC CTGAACCTGT ATCTGAAAAA 
GAAGATGATA GTTATGATGA GGAGGTCCAT GATCCGAGAA GCAGCCGTGG AGGTCTGGTC 
AGTAGAAGGA GTGAGAAGAG TTGGGCAAGG GATAGAAGTG CAAGCAGAGA GAGGAGTTTG 
TCCCCACGGT CTGATAGGCG ATCTGTGGCC TCCAGTCAGC CTGCCAAACC CACCAAAGTC 
ACATTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG TCATATATTT 
GTGAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTAA AGATAAATGG TACCGTGACA GAAAATATGT CACTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGAGCTACC 
CTGTTGAATG TCCCTGATCT TTCTGACAGT ATCCATTCTG CTAATGCCTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA TGACAGACCT 
CCCCGCCACA GCCGGTCACG ATCACCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA 
CACTCTCCAC AGCAGCCCAG CAGTGGCAGT CTCCGGAGCA GAGAGGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCGGATG ATCATACACC TAAAACAGAA 
GAAGTTGTAG TTGAAAGAAA TGAGAAACAA GCACCCACTC TTCCAGAACC AAAGCCTGTA 
TACGCTCAAG TTGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCTTG 
CCTAATTCAA CTCATGAAGA TGGGATTCTG CGGCCCAGCA TGAAATTGGT AAAATTCAGA 
AAAGGAGATA GTGTGGGTCT GCGGCTGGCT GGTGGAAATG ATGTTGGAAT ATTTGTAGCT 
GGTGTTCTAG AAGATAGCCC TGCAGCCAAA GAAGGCTTAG AAGAGGGTGA TCAAATTCTC 
AGGGTAAACA ATGTAGATTT CACAAACATC ATAAGAGAAG AAGCCGTTCT TTTCCTGCTC 
GACCTCCCTA AAGGAGAAGA AGTGACCATA CTGGCTCAGA AAAAGAAAGA TGTTTATCGT 
CGCATCGTTG AATCAGATGT AGGAGATTCT TTCTATATTA GAACCCATTT TGAATATGAA 
AAGGAGTCTC CCTATGGACT TAGTTTTAAC AAAGGAGAGG TGTTCCGTGT TGTGGATACC 
TTGTACAATG GAAAACTAGG CTCTTGGCTT GCTATTCGAA TTGGCAAAAA TCATAAAGAA 
GTAGAACGAG GCATCATCCC CAATAAGAAC AGGGCTGAGC AGTTAGCCAG TGTACAATAC 
ACACTTCCAA AAACAGCAGG AGGAGACCGT GCTGACTTTT GGAGATTCCG GGGTCTTCGC 
AGCTCCAAGA GAAATCTTCG AAAGAGTAGA GAGGATTTGT CAGCTCAGCC AGTTCAAACA 
AAGTTTCCAG CCTATGAAAG AGTTGTTCTT CGAGAAGCTG GATTTCTGAG ACCTGTAACT 
ATCTTTGGAC CCATAGCTGA TGTTGCCAGA GAAAAGCTGG CAAGAGAAGA ACCAGATATT 
TATCAGATTG CAAAAAGTGA ACCACGAGAT GCTGGGACTG ACCAACGTAG CTCTGGCATT 
ATTCGCCTTC ATACGATAAA GCAAATAATA GATCAGGACA AACATGCTTT ATTAGATGTA 
ACCCCAAATG CAGTTGATCG TCTGAATTAT GCCCAGTGGT ATCCAATTGT AGTATTCCTT 
AACCCTGATT CTAAGCAAGG TGTGAAAACT ATGAGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGTGCCA GGAAATTGTA TGAACGGTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGTT GGTATGGTGC ATTGAAAGAA 
GCCATTCAGC AACAGCAAAA CCAGTTGGTA TGGGTTTCTG AAGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAAACTC TTAATGATGA GGTTGGGACT 
CCACCGGAGT CTGCCATTAC ACGGTCCTCT GAGCCTGTAA GAGAGGACTC CTCTGGAATG 
CATCATGAAA ACCAAACATA TCCTCCTTAT TCACCACAAG CGCAGCCACA ACCAATTCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GTCCCTTACC TTTCGCCTGA AACAACCCCA GCATCATCAA CCTCTGCCGT TAATCATAAT 
GTAACTTTAA CTAATGTAAG ACTGGAGGGG CCCACCCCAG CTCCTTCCAC ATCTTACTCA 
CCACAAGCTG ATTCTTTAAG AACACCAAGT ACTGAGGCAG CTCACATAAT GCTAAGAGAT 
CAAGAGCCGT CATTGTCGTC GCATGTAGAT CCAGCAAAGG TGTATAGAAA AGATCCCTAT 
CCTGAGGAAA TGATGAGACA GAATCATGTT TTAAAACAGC CACCCGTTGG TCACCCAGGG 
CAGAGGCCAG ACAAAGAGCC TAATCTGAGC TATGAATCCC AACCCCCATA TGTAGAGAAA 
CAAGCCAGTA GAGACCTTGA ACAGCCTGTG TACAGATACG ACTCCTCTGG CTACCCAGAC 
CAGTTTTCTC GAAACTATGA CCATCGTCTA CGCTATGAAG AGCGCATTCC TACATATGAA 
GAGCAGTGGT CGTATTACGA TGACAAACAG CCCTACCAGC CTCGGCCTTC TTTTGATAAT 
CAGCATCCTC GGGACCTTGA CTCCAGACAG CATCCTGAAG AGTCCTCAGA ACGAGGCTAT 
TACCCACGTT TTGAAGAGCC AGCCCCTCTA CCCTATGACA GCAGACCACG TTATGACCAA 
CCACCTAGAA CCTCCACCCT ACGACATGAA GAGCAGCCAA CTCCTGGATA TGACATGCAT 
AACAGATACA GACCAGAAGC ACAGTCCTAT TCTTCAGCAG GCCCGAAGGC ATCTGAACCC 
AAGCAGTATT TTGACCAATA CCCACGAAGT TATGAGCAAG TACCATCACA AGGATTTACC 
TCTAAAGCAG GCCACTATGA GCCTCTCCAT GGTGCTGCAG TCATCCCTCC TCTAGTACCC 
ACATCTCAAC ATAAGCCAGA AGTTCTGCCT TCAAATACCA AACCACTGCC TCCACCCCCA 
TCTCTGACTG AAGAGGAAGA AGATCCAGCA ATGAAACCCC AGTCTGTGCT CACTAGAGTT 
AAAATGTTTG AAAACAAAAG ATCTGCATCG ATGGAGAATA AGAAGGATGA AAACCACACT 
GCTGGTTTTA AGCCTCCAGA GGTAGCATCT AAACCTCCAG GTGCTGCCAT CGTTGGTCCT 
AAAGCCACTC CTCAGAATCA ATTCAGTGAA CATGACAAAA CACTGTACAG GATCCCAGAA 
CCTCAGAAAC CTCAAATGAA GCCACCTGAA GATATCGTTC GGTCAAATCA TTACGATCCC 
GAGGAGGATG AAGAATATTA TCGAAAGCAA CTCTCCTACT TTGACCGAAG AAGTTTTGAA 
AACAAGCCTT CTACACACAT TCCTACTGGC CATCTCTCAG AGCCTGCCAA GCCAGTTCAT 
TCTCAGAATC AACCAAACTT TTCTAGTTAC TCTTCAAAGG GAAAGTCTCC TGAAGCTGAC 
ACTGCGGATA GATCATTTGG TGAGAAACGC TATGAGCCGA TCCAGGCCAC TCCCCCTCCT 
CCTCCATTGC CCTCCCAGTA TGCCCAGACC AGCTCCTCTC TCGCCCTCCA CACGCATGCC 
AAGGGGGCAC ATGGTGAAGG TAATTCGGTG TCATTGGATT TCCAGAATTC TTTAGTGTCT 
AAACCAGATC CACCTCCGTC TCAGAATAAG CCAGCAACTT TCAGACCACC GAACCGAGAA 
GATACTGTTC AGTCTACTTT CTATCCCCAG AAAAGTTTCC CGGATAAAGC TCCAGTTAAT 
GGAGGTGAAC AGACTCAGAA AACAGTCACT CCAGCATATA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAGCGC AAGTTTGAAA GTCCTAAATT CAACCACAAT 
CTTTTGCCAA GTGAAACTGC ACATAAACCT GATTTGTCAT CAAAAGCCCC TACTTCACCA 
AAAACTCTTG TGAAAGCGCA CAGTGTGGCA CAGCCTCCTG AGTTTGACAG TGGAGTTGAA 
ACTTTCTCCA TCCATGCAGA TAAGCCCAAA TACCAAATGA ATAATGTCAG CACTGTGCCT 
AAAGCTGTTC CTGTGAGCCC TTCAGCTGTG GAAGAAGATG AAGATGAAGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAATGGTG GGGTGTTGAG TTCCATAGAA 
ACCGGCGTTA GTATAATTAT CCCACAAGGA GCCATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGCGAG 
ACACTGCTGA GCCCCTTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTACC TTGTTGGAGC AAACTGCGTT TCTGTCCTGA TAGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110762.1
CDS	16..5589
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_023254994.1
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254994.1

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGAAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCGAA TGGTGCTACT TCTAGCGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATAA AAGGGAGACT CCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTAATCTCA GATGTTCTGA AAGGGGGACC TGCTGAAGGA 
CAGCTACAGG AAAATGACCG GGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGAAAA AGTGGGAAGA ATGCAAAAAT TACCATTAGG 
AGGAAGAAGA AAGTTCAGAT ACCAGTTAGT CGTCCTGATC CTGAACCTGT ATCTGAAAAA 
GAAGATGATA GTTATGATGA GGAGGTCCAT GATCCGAGAA GCAGCCGTGG AGGTCTGGTC 
AGTAGAAGGA GTGAGAAGAG TTGGGCAAGG GATAGAAGTG CAAGCAGAGA GAGGAGTTTG 
TCCCCACGGT CTGATAGGCG ATCTGTGGCC TCCAGTCAGC CTGCCAAACC CACCAAAGTC 
ACATTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG TCATATATTT 
GTGAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTAA AGATAAATGG TACCGTGACA GAAAATATGT CACTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGAGCTACC 
CTGTTGAATG TCCCTGATCT TTCTGACAGT ATCCATTCTG CTAATGCCTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA TGACAGACCT 
CCCCGCCACA GCCGGTCACG ATCACCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA 
CACTCTCCAC AGCAGCCCAG CAGTGGCAGT CTCCGGAGCA GAGAGGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCGGATG ATCATACACC TAAAACAGAA 
GAAGTTGTAG TTGAAAGAAA TGAGAAACAA GCACCCACTC TTCCAGAACC AAAGCCTGTA 
TACGCTCAAG TTGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCTTG 
CCTAATTCAA CTCATGAAGA TGGGATTCTG CGGCCCAGCA TGAAATTGGT AAAATTCAGA 
AAAGGAGATA GTGTGGGTCT GCGGCTGGCT GGTGGAAATG ATGTTGGAAT ATTTGTAGCT 
GGTGTTCTAG AAGATAGCCC TGCAGCCAAA GAAGGCTTAG AAGAGGGTGA TCAAATTCTC 
AGGGTAAACA ATGTAGATTT CACAAACATC ATAAGAGAAG AAGCCGTTCT TTTCCTGCTC 
GACCTCCCTA AAGGAGAAGA AGTGACCATA CTGGCTCAGA AAAAGAAAGA TGTTTATCGT 
CGCATCGTTG AATCAGATGT AGGAGATTCT TTCTATATTA GAACCCATTT TGAATATGAA 
AAGGAGTCTC CCTATGGACT TAGTTTTAAC AAAGGAGAGG TGTTCCGTGT TGTGGATACC 
TTGTACAATG GAAAACTAGG CTCTTGGCTT GCTATTCGAA TTGGCAAAAA TCATAAAGAA 
GTAGAACGAG GCATCATCCC CAATAAGAAC AGGGCTGAGC AGTTAGCCAG TGTACAATAC 
ACACTTCCAA AAACAGCAGG AGGAGACCGT GCTGACTTTT GGAGATTCCG GGGTCTTCGC 
AGCTCCAAGA GAAATCTTCG AAAGAGTAGA GAGGATTTGT CAGCTCAGCC AGTTCAAACA 
AAGTTTCCAG CCTATGAAAG AGTTGTTCTT CGAGAAGCTG GATTTCTGAG ACCTGTAACT 
ATCTTTGGAC CCATAGCTGA TGTTGCCAGA GAAAAGCTGG CAAGAGAAGA ACCAGATATT 
TATCAGATTG CAAAAAGTGA ACCACGAGAT GCTGGGACTG ACCAACGTAG CTCTGGCATT 
ATTCGCCTTC ATACGATAAA GCAAATAATA GATCAGGACA AACATGCTTT ATTAGATGTA 
ACCCCAAATG CAGTTGATCG TCTGAATTAT GCCCAGTGGT ATCCAATTGT AGTATTCCTT 
AACCCTGATT CTAAGCAAGG TGTGAAAACT ATGAGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGTGCCA GGAAATTGTA TGAACGGTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGTT GGTATGGTGC ATTGAAAGAA 
GCCATTCAGC AACAGCAAAA CCAGTTGGTA TGGGTTTCTG AAGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAAACTC TTAATGATGA GGTTGGGACT 
CCACCGGAGT CTGCCATTAC ACGGTCCTCT GAGCCTGTAA GAGAGGACTC CTCTGGAATG 
CATCATGAAA ACCAAACATA TCCTCCTTAT TCACCACAAG CGCAGCCACA ACCAATTCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GTCCCTTACC TTTCGCCTGA AACAACCCCA GCATCATCAA CCTCTGCCGT TAATCATAAT 
GTAACTTTAA CTAATGTAAG ACTGGAGGGG CCCACCCCAG CTCCTTCCAC ATCTTACTCA 
CCACAAGCTG ATTCTTTAAG AACACCAAGT ACTGAGGCAG CTCACATAAT GCTAAGAGAT 
CAAGAGCCGT CATTGTCGTC GCATGTAGAT CCAGCAAAGG TGTATAGAAA AGATCCCTAT 
CCTGAGGAAA TGATGAGACA GAATCATGTT TTAAAACAGC CACCCGTTGG TCACCCAGGG 
CAGAGGCCAG ACAAAGAGCC TAATCTGAGC TATGAATCCC AACCCCCATA TGTAGAGAAA 
CAAGCCAGTA GAGACCTTGA ACAGCCTGTG TACAGATACG ACTCCTCTGG CTACCCAGAC 
CAGTTTTCTC GAAACTATGA CCATCGTCTA CGCTATGAAG AGCGCATTCC TACATATGAA 
GAGCAGTGGT CGTATTACGA TGACAAACAG CCCTACCAGC CTCGGCCTTC TTTTGATAAT 
CAGCATCCTC GGGACCTTGA CTCCAGACAG CATCCTGAAG AGTCCTCAGA ACGAGGCTAT 
TACCCACGTT TTGAAGAGCC AGCCCCTCTA CCCTATGACA GCAGACCACG TTATGACCAA 
CCACCTAGAA CCTCCACCCT ACGACATGAA GAGCAGCCAA CTCCTGGATA TGACATGCAT 
AACAGATACA GACCAGAAGC ACAGTCCTAT TCTTCAGCAG GCCCGAAGGC ATCTGAACCC 
AAGCAGTATT TTGACCAATA CCCACGAAGT TATGAGCAAG TACCATCACA AGGATTTACC 
TCTAAAGCAG GCCACTATGA GCCTCTCCAT GGTGCTGCAG TCATCCCTCC TCTAGTACCC 
ACATCTCAAC ATAAGCCAGA AGTTCTGCCT TCAAATACCA AACCACTGCC TCCACCCCCA 
TCTCTGACTG AAGAGGAAGA AGATCCAGCA ATGAAACCCC AGTCTGTGCT CACTAGAGTT 
AAAATGTTTG AAAACAAAAG ATCTGCATCG ATGGAGAATA AGAAGGATGA AAACCACACT 
GCTGGTTTTA AGCCTCCAGA GGTAGCATCT AAACCTCCAG GTGCTGCCAT CGTTGGTCCT 
AAAGCCACTC CTCAGAATCA ATTCAGTGAA CATGACAAAA CACTGTACAG GATCCCAGAA 
CCTCAGAAAC CTCAAATGAA GCCACCTGAA GATATCGTTC GGTCAAATCA TTACGATCCC 
GAGGAGGATG AAGAATATTA TCGAAAGCAA CTCTCCTACT TTGACCGAAG AAGTTTTGAA 
AACAAGCCTT CTACACACAT TCCTACTGGC CATCTCTCAG AGCCTGCCAA GCCAGTTCAT 
TCTCAGAATC AACCAAACTT TTCTAGTTAC TCTTCAAAGG GAAAGTCTCC TGAAGCTGAC 
ACTGCGGATA GATCATTTGG TGAGAAACGC TATGAGCCGA TCCAGGCCAC TCCCCCTCCT 
CCTCCATTGC CCTCCCAGTA TGCCCAGACC AGCTCCTCTC TCGCCCTCCA CACGCATGCC 
AAGGGGGCAC ATGGTGAAGG TAATTCGGTG TCATTGGATT TCCAGAATTC TTTAGTGTCT 
AAACCAGATC CACCTCCGTC TCAGAATAAG CCAGCAACTT TCAGACCACC GAACCGAGAA 
GATACTGTTC AGTCTACTTT CTATCCCCAG AAAAGTTTCC CGGATAAAGC TCCAGTTAAT 
GGAGGTGAAC AGACTCAGAA AACAGTCACT CCAGCATATA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAGCGC AAGTTTGAAA GTCCTAAATT CAACCACAAT 
CTTTTGCCAA GTGAAACTGC ACATAAACCT GATTTGTCAT CAAAAGCCCC TACTTCACCA 
AAAACTCTTG TGAAAGCGCA CAGTGTGGCA CAGCCTCCTG AGTTTGACAG TGGAGTTGAA 
ACTTTCTCCA TCCATGCAGA TAAGCCCAAA TACCAAATGA ATAATGTCAG CACTGTGCCT 
AAAGCTGTTC CTGTGAGCCC TTCAGCTGTG GAAGAAGATG AAGATGAAGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAATGGTG GGGTGTTGAG TTCCATAGAA 
ACCGGCGTTA GTATAATTAT CCCACAAGGA GCCATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGCGAG 
ACACTGCTGA GCCCCTTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTACC TTGTTGGAGC AAACTGCGTT TCTGTCCTGA TAGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_019831954.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687513.1 Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OFb76459
Clone ID Related Accession (Same CDS sequence)	XM_019831954.2
Accession Version	XM_019831954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019831954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687513.1
CDS	16..5586
Translation	MGEAGARPWFYCCESTTQAEREQRTKQLLEMPLSYKERILKEFNISQVLPRLVYDGVFSLKEYREILSWHCHPRRVESFFLKLCSKGPKAFCAFCSHLEEFCPYLLTCFFLYYQEQTYRILQEASTAEEKARVGTQPEPSDVLETEDQEQSLQLFNKVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_019831954.2
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831954.2

ORF Insert Sequence:

ATGGGTGAGG CAGGGGCCAG GCCCTGGTTC TATTGCTGTG AGTCAACAAC ACAGGCAGAA 
AGGGAGCAGA GGACCAAGCA GCTCTTGGAA ATGCCGCTGA GTTACAAGGA GAGGATACTG 
AAGGAATTCA ACATCAGCCA GGTGCTCCCA CGTCTAGTAT ATGATGGCGT GTTCTCCCTG 
AAGGAGTACA GAGAGATCCT CTCCTGGCAT TGCCACCCAC GGAGAGTGGA GTCCTTTTTT 
CTGAAGCTTT GCTCCAAAGG TCCAAAGGCT TTCTGTGCTT TCTGCTCTCA TCTGGAGGAA 
TTCTGTCCTT ACCTGCTCAC CTGCTTTTTC CTTTATTACC AAGAACAAAC CTATAGGATC 
TTACAAGAGG CATCCACTGC AGAAGAAAAA GCAAGAGTTG GAACTCAGCC TGAACCTAGC 
GATGTCCTTG AGACAGAAGA TCAAGAACAA AGTCTTCAAT TATTTAACAA GGTAACGCCA 
TCCTCTGAAA ATCCGAATGG TGCTACTTCT AGCGTCAGCC AAGGAAAACC TTCTTTAAGA 
CGAATAAAAG GGAGACTCCA CAGAAGCAAA AGCCTTGATA GCATCGATTT CTGTGAACTC 
ACTAACACAG CAATGGAGGA AACAGCTATA TGGGAACAAC ACACAGTGAC GCTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC TCATTTTCAG 
AGTGGGGAAA CGTCAATAGT AATCTCAGAT GTTCTGAAAG GGGGACCTGC TGAAGGACAG 
CTACAGGAAA ATGACCGGGT TGCAATGGTT AATGGAGTTT CAATGGATAA TGTTGAACAT 
GCTTTTGCTG TTCAGCAACT AAGAAAAAGT GGGAAGAATG CAAAAATTAC CATTAGGAGG 
AAGAAGAAAG TTCAGATACC AGTTAGTCGT CCTGATCCTG AACCTGTATC TGAAAAAGAA 
GATGATAGTT ATGATGAGGA GGTCCATGAT CCGAGAAGCA GCCGTGGAGG TCTGGTCAGT 
AGAAGGAGTG AGAAGAGTTG GGCAAGGGAT AGAAGTGCAA GCAGAGAGAG GAGTTTGTCC 
CCACGGTCTG ATAGGCGATC TGTGGCCTCC AGTCAGCCTG CCAAACCCAC CAAAGTCACA 
TTGGTGAAAT CCCGGAAAAA TGAAGAATAT GGTCTTCGAT TGGCCAGTCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTAAAGA TAAATGGTAC CGTGACAGAA AATATGTCAC TGACAGATGC AAAGACATTG 
ATAGAAAGGT CTAAAGGCAA GTTAAAAATG GTAGTTCAGA GAGATGAACG AGCTACCCTG 
TTGAATGTCC CTGATCTTTC TGACAGTATC CATTCTGCTA ATGCCTCTGA GAGAGATGAC 
ATTTCAGAAA TTCAGTCACT GGCATCAGAT CATTCTGGTC GATCACATGA CAGACCTCCC 
CGCCACAGCC GGTCACGATC ACCTGACCAA CGGTCAGAGC CTTCTGATCA TTCCAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCTGGGGCTG TCTCAACTCC TGTAAAGCAT GCGGATGATC ATACACCTAA AACAGAAGAA 
GTTGTAGTTG AAAGAAATGA GAAACAAGCA CCCACTCTTC CAGAACCAAA GCCTGTATAC 
GCTCAAGTTG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG CGTCTTGCCT 
AATTCAACTC ATGAAGATGG GATTCTGCGG CCCAGCATGA AATTGGTAAA ATTCAGAAAA 
GGAGATAGTG TGGGTCTGCG GCTGGCTGGT GGAAATGATG TTGGAATATT TGTAGCTGGT 
GTTCTAGAAG ATAGCCCTGC AGCCAAAGAA GGCTTAGAAG AGGGTGATCA AATTCTCAGG 
GTAAACAATG TAGATTTCAC AAACATCATA AGAGAAGAAG CCGTTCTTTT CCTGCTCGAC 
CTCCCTAAAG GAGAAGAAGT GACCATACTG GCTCAGAAAA AGAAAGATGT TTATCGTCGC 
ATCGTTGAAT CAGATGTAGG AGATTCTTTC TATATTAGAA CCCATTTTGA ATATGAAAAG 
GAGTCTCCCT ATGGACTTAG TTTTAACAAA GGAGAGGTGT TCCGTGTTGT GGATACCTTG 
TACAATGGAA AACTAGGCTC TTGGCTTGCT ATTCGAATTG GCAAAAATCA TAAAGAAGTA 
GAACGAGGCA TCATCCCCAA TAAGAACAGG GCTGAGCAGT TAGCCAGTGT ACAATACACA 
CTTCCAAAAA CAGCAGGAGG AGACCGTGCT GACTTTTGGA GATTCCGGGG TCTTCGCAGC 
TCCAAGAGAA ATCTTCGAAA GAGTAGAGAG GATTTGTCAG CTCAGCCAGT TCAAACAAAG 
TTTCCAGCCT ATGAAAGAGT TGTTCTTCGA GAAGCTGGAT TTCTGAGACC TGTAACTATC 
TTTGGACCCA TAGCTGATGT TGCCAGAGAA AAGCTGGCAA GAGAAGAACC AGATATTTAT 
CAGATTGCAA AAAGTGAACC ACGAGATGCT GGGACTGACC AACGTAGCTC TGGCATTATT 
CGCCTTCATA CGATAAAGCA AATAATAGAT CAGGACAAAC ATGCTTTATT AGATGTAACC 
CCAAATGCAG TTGATCGTCT GAATTATGCC CAGTGGTATC CAATTGTAGT ATTCCTTAAC 
CCTGATTCTA AGCAAGGTGT GAAAACTATG AGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGTGCCAGGA AATTGTATGA ACGGTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGTTGGT ATGGTGCATT GAAAGAAGCC 
ATTCAGCAAC AGCAAAACCA GTTGGTATGG GTTTCTGAAG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAAACTCTTA ATGATGAGGT TGGGACTCCA 
CCGGAGTCTG CCATTACACG GTCCTCTGAG CCTGTAAGAG AGGACTCCTC TGGAATGCAT 
CATGAAAACC AAACATATCC TCCTTATTCA CCACAAGCGC AGCCACAACC AATTCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ATAGAAAAGA TCCCTATCCT 
GAGGAAATGA TGAGACAGAA TCATGTTTTA AAACAGCCAC CCGTTGGTCA CCCAGGGCAG 
AGGCCAGACA AAGAGCCTAA TCTGAGCTAT GAATCCCAAC CCCCATATGT AGAGAAACAA 
GCCAGTAGAG ACCTTGAACA GCCTGTGTAC AGATACGACT CCTCTGGCTA CCCAGACCAG 
TTTTCTCGAA ACTATGACCA TCGTCTACGC TATGAAGAGC GCATTCCTAC ATATGAAGAG 
CAGTGGTCGT ATTACGATGA CAAACAGCCC TACCAGCCTC GGCCTTCTTT TGATAATCAG 
CATCCTCGGG ACCTTGACTC CAGACAGCAT CCTGAAGAGT CCTCAGAACG AGGCTATTAC 
CCACGTTTTG AAGAGCCAGC CCCTCTACCC TATGACAGCA GACCACGTTA TGACCAACCA 
CCTAGAACCT CCACCCTACG ACATGAAGAG CAGCCAACTC CTGGATATGA CATGCATAAC 
AGATACAGAC CAGAAGCACA GTCCTATTCT TCAGCAGGCC CGAAGGCATC TGAACCCAAG 
CAGTATTTTG ACCAATACCC ACGAAGTTAT GAGCAAGTAC CATCACAAGG ATTTACCTCT 
AAAGCAGGCC ACTATGAGCC TCTCCATGGT GCTGCAGTCA TCCCTCCTCT AGTACCCACA 
TCTCAACATA AGCCAGAAGT TCTGCCTTCA AATACCAAAC CACTGCCTCC ACCCCCATCT 
CTGACTGAAG AGGAAGAAGA TCCAGCAATG AAACCCCAGT CTGTGCTCAC TAGAGTTAAA 
ATGTTTGAAA ACAAAAGATC TGCATCGATG GAGAATAAGA AGGATGAAAA CCACACTGCT 
GGTTTTAAGC CTCCAGAGGT AGCATCTAAA CCTCCAGGTG CTGCCATCGT TGGTCCTAAA 
GCCACTCCTC AGAATCAATT CAGTGAACAT GACAAAACAC TGTACAGGAT CCCAGAACCT 
CAGAAACCTC AAATGAAGCC ACCTGAAGAT ATCGTTCGGT CAAATCATTA CGATCCCGAG 
GAGGATGAAG AATATTATCG AAAGCAACTC TCCTACTTTG ACCGAAGAAG TTTTGAAAAC 
AAGCCTTCTA CACACATTCC TACTGGCCAT CTCTCAGAGC CTGCCAAGCC AGTTCATTCT 
CAGAATCAAC CAAACTTTTC TAGTTACTCT TCAAAGGGAA AGTCTCCTGA AGCTGACACT 
GCGGATAGAT CATTTGGTGA GAAACGCTAT GAGCCGATCC AGGCCACTCC CCCTCCTCCT 
CCATTGCCCT CCCAGTATGC CCAGACCAGC TCCTCTCTCG CCCTCCACAC GCATGCCAAG 
GGGGCACATG GTGAAGGTAA TTCGGTGTCA TTGGATTTCC AGAATTCTTT AGTGTCTAAA 
CCAGATCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCGAA CCGAGAAGAT 
ACTGTTCAGT CTACTTTCTA TCCCCAGAAA AGTTTCCCGG ATAAAGCTCC AGTTAATGGA 
GGTGAACAGA CTCAGAAAAC AGTCACTCCA GCATATAATC GATTCACACC AAAACCATAC 
ACAAGTTCTG CTCGGCCATT TGAGCGCAAG TTTGAAAGTC CTAAATTCAA CCACAATCTT 
TTGCCAAGTG AAACTGCACA TAAACCTGAT TTGTCATCAA AAGCCCCTAC TTCACCAAAA 
ACTCTTGTGA AAGCGCACAG TGTGGCACAG CCTCCTGAGT TTGACAGTGG AGTTGAAACT 
TTCTCCATCC ATGCAGATAA GCCCAAATAC CAAATGAATA ATGTCAGCAC TGTGCCTAAA 
GCTGTTCCTG TGAGCCCTTC AGCTGTGGAA GAAGATGAAG ATGAAGATGG TCACACCGTG 
GTGGCCACAG CCCGAGGCGT GTTTAACAGC AATGGTGGGG TGTTGAGTTC CATAGAAACC 
GGCGTTAGTA TAATTATCCC ACAAGGAGCC ATTCCTGAGG GAATTGAGCA GGAAATCTAT 
TTCAAGGTCT GCCGAGACAA CAGCATCCTC CCACCTTTAG ATAAGGAGAA AGGCGAGACA 
CTGCTGAGCC CCTTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCTTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTACCTTG TTGGAGCAAA CTGCGTTTCT GTCCTGATAG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_023254995.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110763.1 Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OFb76460
Clone ID Related Accession (Same CDS sequence)	XM_023254995.1
Accession Version	XM_023254995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGAAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCGAA TGGTGCTACT TCTAGCGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATAA AAGGGAGACT CCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTAATCTCA GATGTTCTGA AAGGGGGACC TGCTGAAGGA 
CAGCTACAGG AAAATGACCG GGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGAAAA AGTGGGAAGA ATGCAAAAAT TACCATTAGG 
AGGAAGAAGA AAGTTCAGAT ACCAGTTAGT CGTCCTGATC CTGAACCTGT ATCTGAAAAA 
GAAGATGATA GTTATGATGA GGAGGTCCAT GATCCGAGAA GCAGCCGTGG AGGTCTGGTC 
AGTAGAAGGA GTGAGAAGAG TTGGGCAAGG GATAGAAGTG CAAGCAGAGA GAGGAGTTTG 
TCCCCACGGT CTGATAGGCG ATCTGTGGCC TCCAGTCAGC CTGCCAAACC CACCAAAGTC 
ACATTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG TCATATATTT 
GTGAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTAA AGATAAATGG TACCGTGACA GAAAATATGT CACTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGAGCTACC 
CTGTTGAATG TCCCTGATCT TTCTGACAGT ATCCATTCTG CTAATGCCTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA TGACAGACCT 
CCCCGCCACA GCCGGTCACG ATCACCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA 
CACTCTCCAC AGCAGCCCAG CAGTGGCAGT CTCCGGAGCA GAGAGGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCGGATG ATCATACACC TAAAACAGAA 
GAAGTTGTAG TTGAAAGAAA TGAGAAACAA GCACCCACTC TTCCAGAACC AAAGCCTGTA 
TACGCTCAAG TTGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCTTG 
CCTAATTCAA CTCATGAAGA TGGGATTCTG CGGCCCAGCA TGAAATTGGT AAAATTCAGA 
AAAGGAGATA GTGTGGGTCT GCGGCTGGCT GGTGGAAATG ATGTTGGAAT ATTTGTAGCT 
GGTGTTCTAG AAGATAGCCC TGCAGCCAAA GAAGGCTTAG AAGAGGGTGA TCAAATTCTC 
AGGGTAAACA ATGTAGATTT CACAAACATC ATAAGAGAAG AAGCCGTTCT TTTCCTGCTC 
GACCTCCCTA AAGGAGAAGA AGTGACCATA CTGGCTCAGA AAAAGAAAGA TGTTTATCGT 
CGCATCGTTG AATCAGATGT AGGAGATTCT TTCTATATTA GAACCCATTT TGAATATGAA 
AAGGAGTCTC CCTATGGACT TAGTTTTAAC AAAGGAGAGG TGTTCCGTGT TGTGGATACC 
TTGTACAATG GAAAACTAGG CTCTTGGCTT GCTATTCGAA TTGGCAAAAA TCATAAAGAA 
GTAGAACGAG GCATCATCCC CAATAAGAAC AGGGCTGAGC AGTTAGCCAG TGTACAATAC 
ACACTTCCAA AAACAGCAGG AGGAGACCGT GCTGACTTTT GGAGATTCCG GGGTCTTCGC 
AGCTCCAAGA GAAATCTTCG AAAGAGTAGA GAGGATTTGT CAGCTCAGCC AGTTCAAACA 
AAGTTTCCAG CCTATGAAAG AGTTGTTCTT CGAGAAGCTG GATTTCTGAG ACCTGTAACT 
ATCTTTGGAC CCATAGCTGA TGTTGCCAGA GAAAAGCTGG CAAGAGAAGA ACCAGATATT 
TATCAGATTG CAAAAAGTGA ACCACGAGAT GCTGGGACTG ACCAACGTAG CTCTGGCATT 
ATTCGCCTTC ATACGATAAA GCAAATAATA GATCAGGACA AACATGCTTT ATTAGATGTA 
ACCCCAAATG CAGTTGATCG TCTGAATTAT GCCCAGTGGT ATCCAATTGT AGTATTCCTT 
AACCCTGATT CTAAGCAAGG TGTGAAAACT ATGAGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGTGCCA GGAAATTGTA TGAACGGTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGTT GGTATGGTGC ATTGAAAGAA 
GCCATTCAGC AACAGCAAAA CCAGTTGGTA TGGGTTTCTG AAGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAAACTC TTAATGATGA GGTTGGGACT 
CCACCGGAGT CTGCCATTAC ACGGTCCTCT GAGCCTGTAA GAGAGGACTC CTCTGGAATG 
CATCATGAAA ACCAAACATA TCCTCCTTAT TCACCACAAG CGCAGCCACA ACCAATTCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GTCCCTTACC TTTCGCCTGA AACAACCCCA GCATCATCAA CCTCTGCCGT TAATCATAAT 
GTAACTTTAA CTAATGTAAG ACTGGAGGGG CCCACCCCAG CTCCTTCCAC ATCTTACTCA 
CCACAAGCTG ATTCTTTAAG AACACCAAGT ACTGAGGCAG CTCACATAAT GCTAAGAGAT 
CAAGAGCCGT CATTGTCGTC GCATGTAGAT CCAGCAAAGG TGTATAGAAA AGATCCCTAT 
CCTGAGGAAA TGATGAGACA GAATCATGTT TTAAAACAGC CACCCGTTGG TCACCCAGGG 
CAGAGGCCAG ACAAAGAGCC TAATCTGAGC TATGAATCCC AACCCCCATA TGTAGAGAAA 
CAAGCCAGTA GAGACCTTGA ACAGCCTGTG TACAGATACG ACTCCTCTGG CTACCCAGAC 
CAGTTTTCTC GAAACTATGA CCATCGTCTA CGCTATGAAG AGCGCATTCC TACATATGAA 
GAGCAGTGGT CGTATTACGA TGACAAACAG CCCTACCAGC CTCGGCCTTC TTTTGATAAT 
CAGCATCCTC GGGACCTTGA CTCCAGACAG CATCCTGAAG AGTCCTCAGA ACGAGGCTAT 
TACCCACGTT TTGAAGAGCC AGCCCCTCTA CCCTATGACA GCAGACCACG TTATGACCAA 
CCACCTAGAA CCTCCACCCT ACGACATGAA GAGCAGCCAA CTCCTGGATA TGACATGCAT 
AACAGATACA GACCAGAAGC ACAGTCCTAT TCTTCAGCAG GCCCGAAGGC ATCTGAACCC 
AAGCAGTATT TTGACCAATA CCCACGAAGT TATGAGCAAG TACCATCACA AGGATTTACC 
TCTAAAGCAG GCCACTATGA GCCTCTCCAT GGTGCTGCAG TCATCCCTCC TCTAGTACCC 
ACATCTCAAC ATAAGCCAGA AGTTCTGCCT TCAAATACCA AACCACTGCC TCCACCCCCA 
TCTCTGACTG AAGAGGAAGA AGATCCAGCA ATGAAACCCC AGTCTGTGCT CACTAGAGTT 
AAAATGTTTG AAAACAAAAG ATCTGCATCG ATGGAGAATA AGAAGGATGA AAACCACACT 
GCTGGTTTTA AGCCTCCAGA GGTAGCATCT AAACCTCCAG GTGCTGCCAT CGTTGGTCCT 
AAAGCCACTC CTCAGAATCA ATTCAGTGAA CATGACAAAA CACTGTACAG GATCCCAGAA 
CCTCAGAAAC CTCAAATGAA GCCACCTGAA GATATCGTTC GGTCAAATCA TTACGATCCC 
GAGGAGGATG AAGAATATTA TCGAAAGCAA CTCTCCTACT TTGACCGAAG AAGTTTTGAA 
AACAAGCCTT CTACACACAT TCCTACTGGC CATCTCTCAG AGCCTGCCAA GCCAGTTCAT 
TCTCAGAATC AACCAAACTT TTCTAGTTAC TCTTCAAAGG GAAAGTCTCC TGAAGCTGAC 
ACTGCGGATA GATCATTTGG TGAGAAACGC TATGAGCCGA TCCAGGCCAC TCCCCCTCCT 
CCTCCATTGC CCTCCCAGTA TGCCCAGACC AGCTCCTCTC TCGCCCTCCA CACGCATGCC 
AAGGGGGCAC ATGGTGAAGG TAATTCGGTG TCATTGGATT TCCAGAATTC TTTAGTGTCT 
AAACCAGATC CACCTCCGTC TCAGAATAAG CCAGCAACTT TCAGACCACC GAACCGAGAA 
GATACTGTTC AGTCTACTTT CTATCCCCAG AAAAGTTTCC CGGATAAAGC TCCAGTTAAT 
GGAGGTGAAC AGACTCAGAA AACAGTCACT CCAGCATATA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAGCGC AAGTTTGAAA GTCCTAAATT CAACCACAAT 
CTTTTGCCAA GTGAAACTGC ACATAAACCT GATTTGTCAT CAAAAGCCCC TACTTCACCA 
AAAACTCTTG TGAAAGCGCA CAGTGTGGCA CAGCCTCCTG AGTTTGACAG TGGAGTTGAA 
ACTTTCTCCA TCCATGCAGA TAAGCCCAAA TACCAAATGA ATAATGTCAG CACTGTGCCT 
AAAGCTGTTC CTGTGAGCCC TTCAGCTGTG GAAGAAGATG AAGATGAAGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAATGGTG GGGTGTTGAG TTCCATAGAA 
ACCGGCGTTA GTATAATTAT CCCACAAGGA GCCATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGCGAG 
ACACTGCTGA GCCCCTTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTACC TTGTTGGAGC AAACTGCGTT TCTGTCCTGA TAGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110763.1
CDS	1..5514
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_023254995.1
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254995.1

ORF Insert Sequence:

ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGCCGGCGGC GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGAAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCGAA TGGTGCTACT TCTAGCGTCA GCCAAGGAAA ACCTTCTTTA 
AGACGAATAA AAGGGAGACT CCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA 
CTCACTAACA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTAATCTCA GATGTTCTGA AAGGGGGACC TGCTGAAGGA 
CAGCTACAGG AAAATGACCG GGTTGCAATG GTTAATGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGAAAA AGTGGGAAGA ATGCAAAAAT TACCATTAGG 
AGGAAGAAGA AAGTTCAGAT ACCAGTTAGT CGTCCTGATC CTGAACCTGT ATCTGAAAAA 
GAAGATGATA GTTATGATGA GGAGGTCCAT GATCCGAGAA GCAGCCGTGG AGGTCTGGTC 
AGTAGAAGGA GTGAGAAGAG TTGGGCAAGG GATAGAAGTG CAAGCAGAGA GAGGAGTTTG 
TCCCCACGGT CTGATAGGCG ATCTGTGGCC TCCAGTCAGC CTGCCAAACC CACCAAAGTC 
ACATTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCCAG TCATATATTT 
GTGAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGCGAT 
GTTGTATTAA AGATAAATGG TACCGTGACA GAAAATATGT CACTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGAGCTACC 
CTGTTGAATG TCCCTGATCT TTCTGACAGT ATCCATTCTG CTAATGCCTC TGAGAGAGAT 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA TGACAGACCT 
CCCCGCCACA GCCGGTCACG ATCACCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA 
CACTCTCCAC AGCAGCCCAG CAGTGGCAGT CTCCGGAGCA GAGAGGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCGGATG ATCATACACC TAAAACAGAA 
GAAGTTGTAG TTGAAAGAAA TGAGAAACAA GCACCCACTC TTCCAGAACC AAAGCCTGTA 
TACGCTCAAG TTGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGCGTCTTG 
CCTAATTCAA CTCATGAAGA TGGGATTCTG CGGCCCAGCA TGAAATTGGT AAAATTCAGA 
AAAGGAGATA GTGTGGGTCT GCGGCTGGCT GGTGGAAATG ATGTTGGAAT ATTTGTAGCT 
GGTGTTCTAG AAGATAGCCC TGCAGCCAAA GAAGGCTTAG AAGAGGGTGA TCAAATTCTC 
AGGGTAAACA ATGTAGATTT CACAAACATC ATAAGAGAAG AAGCCGTTCT TTTCCTGCTC 
GACCTCCCTA AAGGAGAAGA AGTGACCATA CTGGCTCAGA AAAAGAAAGA TGTTTATCGT 
CGCATCGTTG AATCAGATGT AGGAGATTCT TTCTATATTA GAACCCATTT TGAATATGAA 
AAGGAGTCTC CCTATGGACT TAGTTTTAAC AAAGGAGAGG TGTTCCGTGT TGTGGATACC 
TTGTACAATG GAAAACTAGG CTCTTGGCTT GCTATTCGAA TTGGCAAAAA TCATAAAGAA 
GTAGAACGAG GCATCATCCC CAATAAGAAC AGGGCTGAGC AGTTAGCCAG TGTACAATAC 
ACACTTCCAA AAACAGCAGG AGGAGACCGT GCTGACTTTT GGAGATTCCG GGGTCTTCGC 
AGCTCCAAGA GAAATCTTCG AAAGAGTAGA GAGGATTTGT CAGCTCAGCC AGTTCAAACA 
AAGTTTCCAG CCTATGAAAG AGTTGTTCTT CGAGAAGCTG GATTTCTGAG ACCTGTAACT 
ATCTTTGGAC CCATAGCTGA TGTTGCCAGA GAAAAGCTGG CAAGAGAAGA ACCAGATATT 
TATCAGATTG CAAAAAGTGA ACCACGAGAT GCTGGGACTG ACCAACGTAG CTCTGGCATT 
ATTCGCCTTC ATACGATAAA GCAAATAATA GATCAGGACA AACATGCTTT ATTAGATGTA 
ACCCCAAATG CAGTTGATCG TCTGAATTAT GCCCAGTGGT ATCCAATTGT AGTATTCCTT 
AACCCTGATT CTAAGCAAGG TGTGAAAACT ATGAGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGTGCCA GGAAATTGTA TGAACGGTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGTT GGTATGGTGC ATTGAAAGAA 
GCCATTCAGC AACAGCAAAA CCAGTTGGTA TGGGTTTCTG AAGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAAACTC TTAATGATGA GGTTGGGACT 
CCACCGGAGT CTGCCATTAC ACGGTCCTCT GAGCCTGTAA GAGAGGACTC CTCTGGAATG 
CATCATGAAA ACCAAACATA TCCTCCTTAT TCACCACAAG CGCAGCCACA ACCAATTCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GTCCCTTACC TTTCGCCTGA AACAACCCCA GCATCATCAA CCTCTGCCGT TAATCATAAT 
GTAACTTTAA CTAATGTAAG ACTGGAGGGG CCCACCCCAG CTCCTTCCAC ATCTTACTCA 
CCACAAGCTG ATTCTTTAAG AACACCAAGT ACTGAGGCAG CTCACATAAT GCTAAGAGAT 
CAAGAGCCGT CATTGTCGTC GCATGTAGAT CCAGCAAAGG TGTATAGAAA AGATCCCTAT 
CCTGAGGAAA TGATGAGACA GAATCATGTT TTAAAACAGC CACCCGTTGG TCACCCAGGG 
CAGAGGCCAG ACAAAGAGCC TAATCTGAGC TATGAATCCC AACCCCCATA TGTAGAGAAA 
CAAGCCAGTA GAGACCTTGA ACAGCCTGTG TACAGATACG ACTCCTCTGG CTACCCAGAC 
CAGTTTTCTC GAAACTATGA CCATCGTCTA CGCTATGAAG AGCGCATTCC TACATATGAA 
GAGCAGTGGT CGTATTACGA TGACAAACAG CCCTACCAGC CTCGGCCTTC TTTTGATAAT 
CAGCATCCTC GGGACCTTGA CTCCAGACAG CATCCTGAAG AGTCCTCAGA ACGAGGCTAT 
TACCCACGTT TTGAAGAGCC AGCCCCTCTA CCCTATGACA GCAGACCACG TTATGACCAA 
CCACCTAGAA CCTCCACCCT ACGACATGAA GAGCAGCCAA CTCCTGGATA TGACATGCAT 
AACAGATACA GACCAGAAGC ACAGTCCTAT TCTTCAGCAG GCCCGAAGGC ATCTGAACCC 
AAGCAGTATT TTGACCAATA CCCACGAAGT TATGAGCAAG TACCATCACA AGGATTTACC 
TCTAAAGCAG GCCACTATGA GCCTCTCCAT GGTGCTGCAG TCATCCCTCC TCTAGTACCC 
ACATCTCAAC ATAAGCCAGA AGTTCTGCCT TCAAATACCA AACCACTGCC TCCACCCCCA 
TCTCTGACTG AAGAGGAAGA AGATCCAGCA ATGAAACCCC AGTCTGTGCT CACTAGAGTT 
AAAATGTTTG AAAACAAAAG ATCTGCATCG ATGGAGAATA AGAAGGATGA AAACCACACT 
GCTGGTTTTA AGCCTCCAGA GGTAGCATCT AAACCTCCAG GTGCTGCCAT CGTTGGTCCT 
AAAGCCACTC CTCAGAATCA ATTCAGTGAA CATGACAAAA CACTGTACAG GATCCCAGAA 
CCTCAGAAAC CTCAAATGAA GCCACCTGAA GATATCGTTC GGTCAAATCA TTACGATCCC 
GAGGAGGATG AAGAATATTA TCGAAAGCAA CTCTCCTACT TTGACCGAAG AAGTTTTGAA 
AACAAGCCTT CTACACACAT TCCTACTGGC CATCTCTCAG AGCCTGCCAA GCCAGTTCAT 
TCTCAGAATC AACCAAACTT TTCTAGTTAC TCTTCAAAGG GAAAGTCTCC TGAAGCTGAC 
ACTGCGGATA GATCATTTGG TGAGAAACGC TATGAGCCGA TCCAGGCCAC TCCCCCTCCT 
CCTCCATTGC CCTCCCAGTA TGCCCAGACC AGCTCCTCTC TCGCCCTCCA CACGCATGCC 
AAGGGGGCAC ATGGTGAAGG TAATTCGGTG TCATTGGATT TCCAGAATTC TTTAGTGTCT 
AAACCAGATC CACCTCCGTC TCAGAATAAG CCAGCAACTT TCAGACCACC GAACCGAGAA 
GATACTGTTC AGTCTACTTT CTATCCCCAG AAAAGTTTCC CGGATAAAGC TCCAGTTAAT 
GGAGGTGAAC AGACTCAGAA AACAGTCACT CCAGCATATA ATCGATTCAC ACCAAAACCA 
TACACAAGTT CTGCTCGGCC ATTTGAGCGC AAGTTTGAAA GTCCTAAATT CAACCACAAT 
CTTTTGCCAA GTGAAACTGC ACATAAACCT GATTTGTCAT CAAAAGCCCC TACTTCACCA 
AAAACTCTTG TGAAAGCGCA CAGTGTGGCA CAGCCTCCTG AGTTTGACAG TGGAGTTGAA 
ACTTTCTCCA TCCATGCAGA TAAGCCCAAA TACCAAATGA ATAATGTCAG CACTGTGCCT 
AAAGCTGTTC CTGTGAGCCC TTCAGCTGTG GAAGAAGATG AAGATGAAGA TGGTCACACC 
GTGGTGGCCA CAGCCCGAGG CGTGTTTAAC AGCAATGGTG GGGTGTTGAG TTCCATAGAA 
ACCGGCGTTA GTATAATTAT CCCACAAGGA GCCATTCCTG AGGGAATTGA GCAGGAAATC 
TATTTCAAGG TCTGCCGAGA CAACAGCATC CTCCCACCTT TAGATAAGGA GAAAGGCGAG 
ACACTGCTGA GCCCCTTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCT TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTACC TTGTTGGAGC AAACTGCGTT TCTGTCCTGA TAGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_023254996.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110764.1 Felis catus tight junction protein 1 (TJP1), transcript variant X8, mRNA.

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CloneID	OFb76461
Clone ID Related Accession (Same CDS sequence)	XM_023254996.1
Accession Version	XM_023254996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018728.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTAATCTC AGATGTTCTG 
AAAGGGGGAC CTGCTGAAGG ACAGCTACAG GAAAATGACC GGGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGAAA AAGTGGGAAG 
AATGCAAAAA TTACCATTAG GAGGAAGAAG AAAGTTCAGA TACCAGTTAG TCGTCCTGAT 
CCTGAACCTG TATCTGAAAA AGAAGATGAT AGTTATGATG AGGAGGTCCA TGATCCGAGA 
AGCAGCCGTG GAGGTCTGGT CAGTAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCAAGCAGAG AGAGGAGTTT GTCCCCACGG TCTGATAGGC GATCTGTGGC CTCCAGTCAG 
CCTGCCAAAC CCACCAAAGT CACATTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GTCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTA AAGATAAATG GTACCGTGAC AGAAAATATG 
TCACTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGAGCTAC CCTGTTGAAT GTCCCTGATC TTTCTGACAG TATCCATTCT 
GCTAATGCCT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ATGACAGACC TCCCCGCCAC AGCCGGTCAC GATCACCTGA CCAACGGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCGGAT 
GATCATACAC CTAAAACAGA AGAAGTTGTA GTTGAAAGAA ATGAGAAACA AGCACCCACT 
CTTCCAGAAC CAAAGCCTGT ATACGCTCAA GTTGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGCGTCTT GCCTAATTCA ACTCATGAAG ATGGGATTCT GCGGCCCAGC 
ATGAAATTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGTC TGCGGCTGGC TGGTGGAAAT 
GATGTTGGAA TATTTGTAGC TGGTGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA 
GAAGAGGGTG ATCAAATTCT CAGGGTAAAC AATGTAGATT TCACAAACAT CATAAGAGAA 
GAAGCCGTTC TTTTCCTGCT CGACCTCCCT AAAGGAGAAG AAGTGACCAT ACTGGCTCAG 
AAAAAGAAAG ATGTTTATCG TCGCATCGTT GAATCAGATG TAGGAGATTC TTTCTATATT 
AGAACCCATT TTGAATATGA AAAGGAGTCT CCCTATGGAC TTAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TTGTGGATAC CTTGTACAAT GGAAAACTAG GCTCTTGGCT TGCTATTCGA 
ATTGGCAAAA ATCATAAAGA AGTAGAACGA GGCATCATCC CCAATAAGAA CAGGGCTGAG 
CAGTTAGCCA GTGTACAATA CACACTTCCA AAAACAGCAG GAGGAGACCG TGCTGACTTT 
TGGAGATTCC GGGGTCTTCG CAGCTCCAAG AGAAATCTTC GAAAGAGTAG AGAGGATTTG 
TCAGCTCAGC CAGTTCAAAC AAAGTTTCCA GCCTATGAAA GAGTTGTTCT TCGAGAAGCT 
GGATTTCTGA GACCTGTAAC TATCTTTGGA CCCATAGCTG ATGTTGCCAG AGAAAAGCTG 
GCAAGAGAAG AACCAGATAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGGACT 
GACCAACGTA GCTCTGGCAT TATTCGCCTT CATACGATAA AGCAAATAAT AGATCAGGAC 
AAACATGCTT TATTAGATGT AACCCCAAAT GCAGTTGATC GTCTGAATTA TGCCCAGTGG 
TATCCAATTG TAGTATTCCT TAACCCTGAT TCTAAGCAAG GTGTGAAAAC TATGAGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGTGCC AGGAAATTGT ATGAACGGTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGT 
TGGTATGGTG CATTGAAAGA AGCCATTCAG CAACAGCAAA ACCAGTTGGT ATGGGTTTCT 
GAAGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT 
CTTAATGATG AGGTTGGGAC TCCACCGGAG TCTGCCATTA CACGGTCCTC TGAGCCTGTA 
AGAGAGGACT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA TTCACCACAA 
GCGCAGCCAC AACCAATTCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAACCCC AGCATCATCA 
ACCTCTGCCG TTAATCATAA TGTAACTTTA ACTAATGTAA GACTGGAGGG GCCCACCCCA 
GCTCCTTCCA CATCTTACTC ACCACAAGCT GATTCTTTAA GAACACCAAG TACTGAGGCA 
GCTCACATAA TGCTAAGAGA TCAAGAGCCG TCATTGTCGT CGCATGTAGA TCCAGCAAAG 
GTGTATAGAA AAGATCCCTA TCCTGAGGAA ATGATGAGAC AGAATCATGT TTTAAAACAG 
CCACCCGTTG GTCACCCAGG GCAGAGGCCA GACAAAGAGC CTAATCTGAG CTATGAATCC 
CAACCCCCAT ATGTAGAGAA ACAAGCCAGT AGAGACCTTG AACAGCCTGT GTACAGATAC 
GACTCCTCTG GCTACCCAGA CCAGTTTTCT CGAAACTATG ACCATCGTCT ACGCTATGAA 
GAGCGCATTC CTACATATGA AGAGCAGTGG TCGTATTACG ATGACAAACA GCCCTACCAG 
CCTCGGCCTT CTTTTGATAA TCAGCATCCT CGGGACCTTG ACTCCAGACA GCATCCTGAA 
GAGTCCTCAG AACGAGGCTA TTACCCACGT TTTGAAGAGC CAGCCCCTCT ACCCTATGAC 
AGCAGACCAC GTTATGACCA ACCACCTAGA ACCTCCACCC TACGACATGA AGAGCAGCCA 
ACTCCTGGAT ATGACATGCA TAACAGATAC AGACCAGAAG CACAGTCCTA TTCTTCAGCA 
GGCCCGAAGG CATCTGAACC CAAGCAGTAT TTTGACCAAT ACCCACGAAG TTATGAGCAA 
GTACCATCAC AAGGATTTAC CTCTAAAGCA GGCCACTATG AGCCTCTCCA TGGTGCTGCA 
GTCATCCCTC CTCTAGTACC CACATCTCAA CATAAGCCAG AAGTTCTGCC TTCAAATACC 
AAACCACTGC CTCCACCCCC ATCTCTGACT GAAGAGGAAG AAGATCCAGC AATGAAACCC 
CAGTCTGTGC TCACTAGAGT TAAAATGTTT GAAAACAAAA GATCTGCATC GATGGAGAAT 
AAGAAGGATG AAAACCACAC TGCTGGTTTT AAGCCTCCAG AGGTAGCATC TAAACCTCCA 
GGTGCTGCCA TCGTTGGTCC TAAAGCCACT CCTCAGAATC AATTCAGTGA ACATGACAAA 
ACACTGTACA GGATCCCAGA ACCTCAGAAA CCTCAAATGA AGCCACCTGA AGATATCGTT 
CGGTCAAATC ATTACGATCC CGAGGAGGAT GAAGAATATT ATCGAAAGCA ACTCTCCTAC 
TTTGACCGAA GAAGTTTTGA AAACAAGCCT TCTACACACA TTCCTACTGG CCATCTCTCA 
GAGCCTGCCA AGCCAGTTCA TTCTCAGAAT CAACCAAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAGTCTC CTGAAGCTGA CACTGCGGAT AGATCATTTG GTGAGAAACG CTATGAGCCG 
ATCCAGGCCA CTCCCCCTCC TCCTCCATTG CCCTCCCAGT ATGCCCAGAC CAGCTCCTCT 
CTCGCCCTCC ACACGCATGC CAAGGGGGCA CATGGTGAAG GTAATTCGGT GTCATTGGAT 
TTCCAGAATT CTTTAGTGTC TAAACCAGAT CCACCTCCGT CTCAGAATAA GCCAGCAACT 
TTCAGACCAC CGAACCGAGA AGATACTGTT CAGTCTACTT TCTATCCCCA GAAAAGTTTC 
CCGGATAAAG CTCCAGTTAA TGGAGGTGAA CAGACTCAGA AAACAGTCAC TCCAGCATAT 
AATCGATTCA CACCAAAACC ATACACAAGT TCTGCTCGGC CATTTGAGCG CAAGTTTGAA 
AGTCCTAAAT TCAACCACAA TCTTTTGCCA AGTGAAACTG CACATAAACC TGATTTGTCA 
TCAAAAGCCC CTACTTCACC AAAAACTCTT GTGAAAGCGC ACAGTGTGGC ACAGCCTCCT 
GAGTTTGACA GTGGAGTTGA AACTTTCTCC ATCCATGCAG ATAAGCCCAA ATACCAAATG 
AATAATGTCA GCACTGTGCC TAAAGCTGTT CCTGTGAGCC CTTCAGCTGT GGAAGAAGAT 
GAAGATGAAG ATGGTCACAC CGTGGTGGCC ACAGCCCGAG GCGTGTTTAA CAGCAATGGT 
GGGGTGTTGA GTTCCATAGA AACCGGCGTT AGTATAATTA TCCCACAAGG AGCCATTCCT 
GAGGGAATTG AGCAGGAAAT CTATTTCAAG GTCTGCCGAG ACAACAGCAT CCTCCCACCT 
TTAGATAAGG AGAAAGGCGA GACACTGCTG AGCCCCTTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC TTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAC CTTGTTGGAG CAAACTGCGT TTCTGTCCTG 
ATAGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110764.1
CDS	114..5408
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSEKEDDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTEEVVVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETTPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPAKVYRKDPYPEEMMRQNHVLKQPPVGHPGQRPDKEPNLSYESQPPYVEKQASRDLEQPVYRYDSSGYPDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYYPRFEEPAPLPYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFTSKAGHYEPLHGAAVIPPLVPTSQHKPEVLPSNTKPLPPPPSLTEEEEDPAMKPQSVLTRVKMFENKRSASMENKKDENHTAGFKPPEVASKPPGAAIVGPKATPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPTGHLSEPAKPVHSQNQPNFSSYSSKGKSPEADTADRSFGEKRYEPIQATPPPPPLPSQYAQTSSSLALHTHAKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGGEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPTSPKTLVKAHSVAQPPEFDSGVETFSIHADKPKYQMNNVSTVPKAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_023254996.1
Organism	Felis catus(domestic cat)
Definition	Felis catus tight junction protein 1 (TJP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254996.1

ORF Insert Sequence:

ATGTCCGCCA GAGCCGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTAATCTC AGATGTTCTG 
AAAGGGGGAC CTGCTGAAGG ACAGCTACAG GAAAATGACC GGGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGAAA AAGTGGGAAG 
AATGCAAAAA TTACCATTAG GAGGAAGAAG AAAGTTCAGA TACCAGTTAG TCGTCCTGAT 
CCTGAACCTG TATCTGAAAA AGAAGATGAT AGTTATGATG AGGAGGTCCA TGATCCGAGA 
AGCAGCCGTG GAGGTCTGGT CAGTAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCAAGCAGAG AGAGGAGTTT GTCCCCACGG TCTGATAGGC GATCTGTGGC CTCCAGTCAG 
CCTGCCAAAC CCACCAAAGT CACATTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GTCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTA AAGATAAATG GTACCGTGAC AGAAAATATG 
TCACTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT 
CAGAGAGATG AACGAGCTAC CCTGTTGAAT GTCCCTGATC TTTCTGACAG TATCCATTCT 
GCTAATGCCT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ATGACAGACC TCCCCGCCAC AGCCGGTCAC GATCACCTGA CCAACGGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCGGAT 
GATCATACAC CTAAAACAGA AGAAGTTGTA GTTGAAAGAA ATGAGAAACA AGCACCCACT 
CTTCCAGAAC CAAAGCCTGT ATACGCTCAA GTTGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGCGTCTT GCCTAATTCA ACTCATGAAG ATGGGATTCT GCGGCCCAGC 
ATGAAATTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGTC TGCGGCTGGC TGGTGGAAAT 
GATGTTGGAA TATTTGTAGC TGGTGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA 
GAAGAGGGTG ATCAAATTCT CAGGGTAAAC AATGTAGATT TCACAAACAT CATAAGAGAA 
GAAGCCGTTC TTTTCCTGCT CGACCTCCCT AAAGGAGAAG AAGTGACCAT ACTGGCTCAG 
AAAAAGAAAG ATGTTTATCG TCGCATCGTT GAATCAGATG TAGGAGATTC TTTCTATATT 
AGAACCCATT TTGAATATGA AAAGGAGTCT CCCTATGGAC TTAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TTGTGGATAC CTTGTACAAT GGAAAACTAG GCTCTTGGCT TGCTATTCGA 
ATTGGCAAAA ATCATAAAGA AGTAGAACGA GGCATCATCC CCAATAAGAA CAGGGCTGAG 
CAGTTAGCCA GTGTACAATA CACACTTCCA AAAACAGCAG GAGGAGACCG TGCTGACTTT 
TGGAGATTCC GGGGTCTTCG CAGCTCCAAG AGAAATCTTC GAAAGAGTAG AGAGGATTTG 
TCAGCTCAGC CAGTTCAAAC AAAGTTTCCA GCCTATGAAA GAGTTGTTCT TCGAGAAGCT 
GGATTTCTGA GACCTGTAAC TATCTTTGGA CCCATAGCTG ATGTTGCCAG AGAAAAGCTG 
GCAAGAGAAG AACCAGATAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGGACT 
GACCAACGTA GCTCTGGCAT TATTCGCCTT CATACGATAA AGCAAATAAT AGATCAGGAC 
AAACATGCTT TATTAGATGT AACCCCAAAT GCAGTTGATC GTCTGAATTA TGCCCAGTGG 
TATCCAATTG TAGTATTCCT TAACCCTGAT TCTAAGCAAG GTGTGAAAAC TATGAGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGTGCC AGGAAATTGT ATGAACGGTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGT 
TGGTATGGTG CATTGAAAGA AGCCATTCAG CAACAGCAAA ACCAGTTGGT ATGGGTTTCT 
GAAGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT 
CTTAATGATG AGGTTGGGAC TCCACCGGAG TCTGCCATTA CACGGTCCTC TGAGCCTGTA 
AGAGAGGACT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA TTCACCACAA 
GCGCAGCCAC AACCAATTCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAACCCC AGCATCATCA 
ACCTCTGCCG TTAATCATAA TGTAACTTTA ACTAATGTAA GACTGGAGGG GCCCACCCCA 
GCTCCTTCCA CATCTTACTC ACCACAAGCT GATTCTTTAA GAACACCAAG TACTGAGGCA 
GCTCACATAA TGCTAAGAGA TCAAGAGCCG TCATTGTCGT CGCATGTAGA TCCAGCAAAG 
GTGTATAGAA AAGATCCCTA TCCTGAGGAA ATGATGAGAC AGAATCATGT TTTAAAACAG 
CCACCCGTTG GTCACCCAGG GCAGAGGCCA GACAAAGAGC CTAATCTGAG CTATGAATCC 
CAACCCCCAT ATGTAGAGAA ACAAGCCAGT AGAGACCTTG AACAGCCTGT GTACAGATAC 
GACTCCTCTG GCTACCCAGA CCAGTTTTCT CGAAACTATG ACCATCGTCT ACGCTATGAA 
GAGCGCATTC CTACATATGA AGAGCAGTGG TCGTATTACG ATGACAAACA GCCCTACCAG 
CCTCGGCCTT CTTTTGATAA TCAGCATCCT CGGGACCTTG ACTCCAGACA GCATCCTGAA 
GAGTCCTCAG AACGAGGCTA TTACCCACGT TTTGAAGAGC CAGCCCCTCT ACCCTATGAC 
AGCAGACCAC GTTATGACCA ACCACCTAGA ACCTCCACCC TACGACATGA AGAGCAGCCA 
ACTCCTGGAT ATGACATGCA TAACAGATAC AGACCAGAAG CACAGTCCTA TTCTTCAGCA 
GGCCCGAAGG CATCTGAACC CAAGCAGTAT TTTGACCAAT ACCCACGAAG TTATGAGCAA 
GTACCATCAC AAGGATTTAC CTCTAAAGCA GGCCACTATG AGCCTCTCCA TGGTGCTGCA 
GTCATCCCTC CTCTAGTACC CACATCTCAA CATAAGCCAG AAGTTCTGCC TTCAAATACC 
AAACCACTGC CTCCACCCCC ATCTCTGACT GAAGAGGAAG AAGATCCAGC AATGAAACCC 
CAGTCTGTGC TCACTAGAGT TAAAATGTTT GAAAACAAAA GATCTGCATC GATGGAGAAT 
AAGAAGGATG AAAACCACAC TGCTGGTTTT AAGCCTCCAG AGGTAGCATC TAAACCTCCA 
GGTGCTGCCA TCGTTGGTCC TAAAGCCACT CCTCAGAATC AATTCAGTGA ACATGACAAA 
ACACTGTACA GGATCCCAGA ACCTCAGAAA CCTCAAATGA AGCCACCTGA AGATATCGTT 
CGGTCAAATC ATTACGATCC CGAGGAGGAT GAAGAATATT ATCGAAAGCA ACTCTCCTAC 
TTTGACCGAA GAAGTTTTGA AAACAAGCCT TCTACACACA TTCCTACTGG CCATCTCTCA 
GAGCCTGCCA AGCCAGTTCA TTCTCAGAAT CAACCAAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAGTCTC CTGAAGCTGA CACTGCGGAT AGATCATTTG GTGAGAAACG CTATGAGCCG 
ATCCAGGCCA CTCCCCCTCC TCCTCCATTG CCCTCCCAGT ATGCCCAGAC CAGCTCCTCT 
CTCGCCCTCC ACACGCATGC CAAGGGGGCA CATGGTGAAG GTAATTCGGT GTCATTGGAT 
TTCCAGAATT CTTTAGTGTC TAAACCAGAT CCACCTCCGT CTCAGAATAA GCCAGCAACT 
TTCAGACCAC CGAACCGAGA AGATACTGTT CAGTCTACTT TCTATCCCCA GAAAAGTTTC 
CCGGATAAAG CTCCAGTTAA TGGAGGTGAA CAGACTCAGA AAACAGTCAC TCCAGCATAT 
AATCGATTCA CACCAAAACC ATACACAAGT TCTGCTCGGC CATTTGAGCG CAAGTTTGAA 
AGTCCTAAAT TCAACCACAA TCTTTTGCCA AGTGAAACTG CACATAAACC TGATTTGTCA 
TCAAAAGCCC CTACTTCACC AAAAACTCTT GTGAAAGCGC ACAGTGTGGC ACAGCCTCCT 
GAGTTTGACA GTGGAGTTGA AACTTTCTCC ATCCATGCAG ATAAGCCCAA ATACCAAATG 
AATAATGTCA GCACTGTGCC TAAAGCTGTT CCTGTGAGCC CTTCAGCTGT GGAAGAAGAT 
GAAGATGAAG ATGGTCACAC CGTGGTGGCC ACAGCCCGAG GCGTGTTTAA CAGCAATGGT 
GGGGTGTTGA GTTCCATAGA AACCGGCGTT AGTATAATTA TCCCACAAGG AGCCATTCCT 
GAGGGAATTG AGCAGGAAAT CTATTTCAAG GTCTGCCGAG ACAACAGCAT CCTCCCACCT 
TTAGATAAGG AGAAAGGCGA GACACTGCTG AGCCCCTTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC TTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAC CTTGTTGGAG CAAACTGCGT TTCTGTCCTG 
ATAGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TJP1
Entrez Gene ID	101082238
Full Name	tight junction protein 1
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TJP1 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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