EHI_052050 cDNA ORF clone, Entamoeba histolytica HM-1:IMSS

The following EHI_052050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHI_052050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OEb00712 XM_650138.1
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Entamoeba histolytica HM-1:IMSS 26s protease regulatory subunit (EHI_052050), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OEb00712
    Clone ID Related Accession (Same CDS sequence) XM_650138.1
    Accession Version XM_650138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1508774400000
    Organism Entamoeba histolytica HM-1:IMSS
    Product 26s protease regulatory subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001915062). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAAGAAG ATTTTGAGAA GAAACCAAAA ACACTTGATG AAGAAGAAAT TGCTATTTTA 
    AAAGCATATA ATCGTGGTCC ATATGCAAAT TCAATTAAAC AATTAGAAAA AGAAGTTGCA
    GACCTTGCAA CAGAAATACA TAATTTAGTT GGAATACAAG AAAGTGATAC AGGACTAGCA
    CCAGTGTCTC AATGGGATTT AAATGCTGAT AAAAAATTGA TGGAAGAACA ACCATTACTT
    GTTTCTAGAT GTATTAAAGC TATGCCAGAT GAACGTGAAC CACGATATGT TATTTCTATA
    AAAGAATATG CAAAATTTGT TGTTGGTAAA TCAAATAGAG TTGAAAAAGA TGCCGTTCAA
    GATGGAACAA GAGTCGGAGT AGATAGAGCA CGTTATGAAA TTAAAATGGC ACTTCCACCA
    AAAATTGACC CATCAGTTTC TGTTATGCAA GTTGAAGAAA AACCAGATGT AACATACAAA
    GATATTGGAG GGTGTAAAGA ACAAATTGAA AGAATTAGAG AAGTTGTTGA ACTTCCTATG
    TTACATCCAG AGGCATTTGA GAATTTAGGA ATTGATCCAC CTAAAGGAGT ATTATTATAT
    GGACCACCAG GAACAGGTAA AACATTATTA GCACGTGCTG TAGCTAATAG AACAGAATCA
    ACCTTTGTTC GAGTAATTGG ATCAGAGTTA GTTCAAAAAT ATGTTGGAGA AGGTGCTAAA
    ATGGTAAGAG ACTTATTTGA CATGGCTAAA AGTAAAAAGA GTTGTATTAT TTTCTTTGAT
    GAAATTGATG CTATTGGAGG AACTAGATTC CAAGATGATA CAGGTGAAAG TGAAGTACAA
    CGTACTATGT TAGAACTTAT TAATCAATTA GATGGATTTG ATAAACGTGG AAATATTAAA
    GTCCTTATGG CAACAAATAG ACCAGACACA CTTGATCCAG CATTGGTTAG ACCAGGAAGA
    CTTGATAGAA AGATTGAATT TGGTCTTCCA GATATTGAAG GAAGAACAGA AATATTTAAA
    ATTCATACAA AGCCAATGAG TGTTGCAAAA GACATTCGTT ATGACCTTTT AGCCAGACTT
    TGCCCAAATG CCACTGGAGC AGAAATTCAA TCAGTTTGTA CTGAAGCAGG AATGTTTGCA
    ATTAGAGCAA GAAGAAAAGT TGTTACTGAA CGAGATTTCT TAGATGCAGT AGAAAAAGTT
    ATTAAAGGTT ATCAAAAATT CTCTGCAACT CCAAAATATC TTGCTTACAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_655230.1
    CDS1..1254
    Translation

    Target ORF information:

    RefSeq Version XM_650138.1
    Organism Entamoeba histolytica HM-1:IMSS
    Definition Entamoeba histolytica HM-1:IMSS 26s protease regulatory subunit (EHI_052050), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_650138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAAGAAG ATTTTGAGAA GAAACCAAAA ACACTTGATG AAGAAGAAAT TGCTATTTTA 
    AAAGCATATA ATCGTGGTCC ATATGCAAAT TCAATTAAAC AATTAGAAAA AGAAGTTGCA
    GACCTTGCAA CAGAAATACA TAATTTAGTT GGAATACAAG AAAGTGATAC AGGACTAGCA
    CCAGTGTCTC AATGGGATTT AAATGCTGAT AAAAAATTGA TGGAAGAACA ACCATTACTT
    GTTTCTAGAT GTATTAAAGC TATGCCAGAT GAACGTGAAC CACGATATGT TATTTCTATA
    AAAGAATATG CAAAATTTGT TGTTGGTAAA TCAAATAGAG TTGAAAAAGA TGCCGTTCAA
    GATGGAACAA GAGTCGGAGT AGATAGAGCA CGTTATGAAA TTAAAATGGC ACTTCCACCA
    AAAATTGACC CATCAGTTTC TGTTATGCAA GTTGAAGAAA AACCAGATGT AACATACAAA
    GATATTGGAG GGTGTAAAGA ACAAATTGAA AGAATTAGAG AAGTTGTTGA ACTTCCTATG
    TTACATCCAG AGGCATTTGA GAATTTAGGA ATTGATCCAC CTAAAGGAGT ATTATTATAT
    GGACCACCAG GAACAGGTAA AACATTATTA GCACGTGCTG TAGCTAATAG AACAGAATCA
    ACCTTTGTTC GAGTAATTGG ATCAGAGTTA GTTCAAAAAT ATGTTGGAGA AGGTGCTAAA
    ATGGTAAGAG ACTTATTTGA CATGGCTAAA AGTAAAAAGA GTTGTATTAT TTTCTTTGAT
    GAAATTGATG CTATTGGAGG AACTAGATTC CAAGATGATA CAGGTGAAAG TGAAGTACAA
    CGTACTATGT TAGAACTTAT TAATCAATTA GATGGATTTG ATAAACGTGG AAATATTAAA
    GTCCTTATGG CAACAAATAG ACCAGACACA CTTGATCCAG CATTGGTTAG ACCAGGAAGA
    CTTGATAGAA AGATTGAATT TGGTCTTCCA GATATTGAAG GAAGAACAGA AATATTTAAA
    ATTCATACAA AGCCAATGAG TGTTGCAAAA GACATTCGTT ATGACCTTTT AGCCAGACTT
    TGCCCAAATG CCACTGGAGC AGAAATTCAA TCAGTTTGTA CTGAAGCAGG AATGTTTGCA
    ATTAGAGCAA GAAGAAAAGT TGTTACTGAA CGAGATTTCT TAGATGCAGT AGAAAAAGTT
    ATTAAAGGTT ATCAAAAATT CTCTGCAACT CCAAAATATC TTGCTTACAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the protist parasite Entamoeba histolytica.
    Nature433(7028)865-8(2005 Feb)
    Loftus B,Anderson I,Davies R,Alsmark UC,Samuelson J,Amedeo P,Roncaglia P,Berriman M,Hirt RP,Mann BJ,Nozaki T,Suh B,Pop M,Duchene M,Ackers J,Tannich E,Leippe M,Hofer M,Bruchhaus I,Willhoeft U,Bhattacharya A,Chillingworth T,Churcher C,Hance Z,Harris B,Harris D,Jagels K,Moule S,Mungall K,Ormond D,Squares R,Whitehead S,Quail MA,Rabbinowitsch E,Norbertczak H,Price C,Wang Z,Guillén N,Gilchrist C,Stroup SE,Bhattacharya S,Lohia A,Foster PG,Sicheritz-Ponten T,Weber C,Singh U,Mukherjee C,El-Sayed NM,Petri WA Jr,Clark CG,Embley TM,Barrell B,Fraser CM,Hall N