EHI_103270 cDNA ORF clone, Entamoeba histolytica HM-1:IMSS

The following EHI_103270 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHI_103270 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OEb03557 XM_647344.1
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Entamoeba histolytica HM-1:IMSS T-complex protein 1 beta subunit, putative (EHI_103270), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OEb03557
    Clone ID Related Accession (Same CDS sequence) XM_647344.1
    Accession Version XM_647344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1508774400000
    Organism Entamoeba histolytica HM-1:IMSS
    Product T-complex protein 1 beta subunit, putative
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001914873). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCAAGGAA CACAATTCGA TTCTACACCA GTTCGAGTAC TTAAAAGTGA TACCCAACAT 
    GTTGTTGGTG ATACAGCACG AATGACTGCA ACAGTAGGTG CTATTGCTAT TGCAGATTTA
    GTTAAAACAA CGTTAGGACC AATGGGAATG GATAAAATAT TAATTGGTTC ATCACAAGCT
    GATAATATTA CTGTTACTAA TGATGGAGCA ACTATTCTCA AATCAGTTAG ATTAGAAAAC
    CCTGCTGCTA AAGTTCTTGT TGAAATGAGT AAAACAACTG ATGAAGAAGT TGGAGATGGA
    ACAACATCAG TAACCGTATT AGCTGGAGAA TTAATGAGAG AAGCAGAGAA ATTAATGGCA
    GAAAAAATTC ATCCGCAAGT TATTATTGAG GGATGGAGAA TTGCTTTAAA GGCTGCACAA
    GAAAAATTAG AAACTATGCA ATTTGATGTT TCAAGTGACC ACGAAGCATA TAAAAAAAGA
    CTAATGGATA TTGCACGTAC TACTTTATCA TCAAAAATTC TTTCAGCAGA AAAAGAAAAA
    TTTGCACAAA TTGCAGTTGA TGCTATTGAA AAGATTGGAG ATGCTGACAT TGACAATGTT
    GTTATTCTCC AAAAGATTGG AGGAGAAATG AGAGAGTCAT ATTTAGAAGA TGGATTCTTA
    TTAGAAAAGA AATTTGGAAC AGCACAAAAA CATAAAGTAG AAAATCCAAA AATCATGGTT
    GCTAATACTG CTATGGATAA TGACAGAATT AAAATTTGGA GTGGTAAAGT TAAAGTTGAT
    AGTGTTTCAA AAGTAGCTGA AATTGAAGAG GCTGAGAGAA GTCGTATGTT AAAGAAATGT
    GAACAAATTA AAGCACATGG AGTTACTTGT TTTGTTTCAA GACAATTAAT ATATCCAATT
    CCAGAACAAT TCTTTAACGC TAATGGTATT GTTTCAATTG ATCATGCTGA CTTTGATGGA
    GTTGAAAGAA TTGCAGCATT ATCAGGTGCA GAAATTTTAT CTACTTTTGA CCATCCTGAT
    GAAGCTAAAT TAGGTACATG CAAAAGTATT GAAGAAATTA TGATTGGTGA AGAGACTTTA
    ATTAGATTTA GTGGATTTCC TCATGGTGGA GCTTGTACTG TTGTATTAAG AGGTCCATCT
    AAACAATTAT TAGGAGAAAT GGAAAGAAGT CTTCATGACG CATTATGTGT TTTACATATT
    ATTCGTAAAG ATACTAAAAT GATTTGTGGA GGTGGTTGTG TTGAAATGGA ATTAAGTTTA
    GCAGTTGAAG AAGCTGCTAA AAAAACCGAA GGCAAAAAAG CTTTAGCAGT TGAGGCTTTT
    GCTCGTGCCT TAAGACAACT ACCAATGATC ATTGCTGATA ATGCTGGTTA TGACTCAGCT
    GATCTTGTTG CTCAATTACG TGCTGCTCAT GCAAATAACT CAATTCAAAA TGCTGGTTTA
    GATATGGAAC ATGGTACTAT TGCTGATATG AAACAAATTG GTATTATTGA ACCTTTTAGA
    GTGAAAAAAC ACATTTTAAC TGCTGCTACT GAAGCTGCTG AAATGATTAT TCGTGTTGAT
    CATATTATTC AGGCTGCTCC TAGAAAAAGA GAACAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_652436.1
    CDS1..1602
    Translation

    Target ORF information:

    RefSeq Version XM_647344.1
    Organism Entamoeba histolytica HM-1:IMSS
    Definition Entamoeba histolytica HM-1:IMSS T-complex protein 1 beta subunit, putative (EHI_103270), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_647344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCAAGGAA CACAATTCGA TTCTACACCA GTTCGAGTAC TTAAAAGTGA TACCCAACAT 
    GTTGTTGGTG ATACAGCACG AATGACTGCA ACAGTAGGTG CTATTGCTAT TGCAGATTTA
    GTTAAAACAA CGTTAGGACC AATGGGAATG GATAAAATAT TAATTGGTTC ATCACAAGCT
    GATAATATTA CTGTTACTAA TGATGGAGCA ACTATTCTCA AATCAGTTAG ATTAGAAAAC
    CCTGCTGCTA AAGTTCTTGT TGAAATGAGT AAAACAACTG ATGAAGAAGT TGGAGATGGA
    ACAACATCAG TAACCGTATT AGCTGGAGAA TTAATGAGAG AAGCAGAGAA ATTAATGGCA
    GAAAAAATTC ATCCGCAAGT TATTATTGAG GGATGGAGAA TTGCTTTAAA GGCTGCACAA
    GAAAAATTAG AAACTATGCA ATTTGATGTT TCAAGTGACC ACGAAGCATA TAAAAAAAGA
    CTAATGGATA TTGCACGTAC TACTTTATCA TCAAAAATTC TTTCAGCAGA AAAAGAAAAA
    TTTGCACAAA TTGCAGTTGA TGCTATTGAA AAGATTGGAG ATGCTGACAT TGACAATGTT
    GTTATTCTCC AAAAGATTGG AGGAGAAATG AGAGAGTCAT ATTTAGAAGA TGGATTCTTA
    TTAGAAAAGA AATTTGGAAC AGCACAAAAA CATAAAGTAG AAAATCCAAA AATCATGGTT
    GCTAATACTG CTATGGATAA TGACAGAATT AAAATTTGGA GTGGTAAAGT TAAAGTTGAT
    AGTGTTTCAA AAGTAGCTGA AATTGAAGAG GCTGAGAGAA GTCGTATGTT AAAGAAATGT
    GAACAAATTA AAGCACATGG AGTTACTTGT TTTGTTTCAA GACAATTAAT ATATCCAATT
    CCAGAACAAT TCTTTAACGC TAATGGTATT GTTTCAATTG ATCATGCTGA CTTTGATGGA
    GTTGAAAGAA TTGCAGCATT ATCAGGTGCA GAAATTTTAT CTACTTTTGA CCATCCTGAT
    GAAGCTAAAT TAGGTACATG CAAAAGTATT GAAGAAATTA TGATTGGTGA AGAGACTTTA
    ATTAGATTTA GTGGATTTCC TCATGGTGGA GCTTGTACTG TTGTATTAAG AGGTCCATCT
    AAACAATTAT TAGGAGAAAT GGAAAGAAGT CTTCATGACG CATTATGTGT TTTACATATT
    ATTCGTAAAG ATACTAAAAT GATTTGTGGA GGTGGTTGTG TTGAAATGGA ATTAAGTTTA
    GCAGTTGAAG AAGCTGCTAA AAAAACCGAA GGCAAAAAAG CTTTAGCAGT TGAGGCTTTT
    GCTCGTGCCT TAAGACAACT ACCAATGATC ATTGCTGATA ATGCTGGTTA TGACTCAGCT
    GATCTTGTTG CTCAATTACG TGCTGCTCAT GCAAATAACT CAATTCAAAA TGCTGGTTTA
    GATATGGAAC ATGGTACTAT TGCTGATATG AAACAAATTG GTATTATTGA ACCTTTTAGA
    GTGAAAAAAC ACATTTTAAC TGCTGCTACT GAAGCTGCTG AAATGATTAT TCGTGTTGAT
    CATATTATTC AGGCTGCTCC TAGAAAAAGA GAACAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the protist parasite Entamoeba histolytica.
    Nature433(7028)865-8(2005 Feb)
    Loftus B,Anderson I,Davies R,Alsmark UC,Samuelson J,Amedeo P,Roncaglia P,Berriman M,Hirt RP,Mann BJ,Nozaki T,Suh B,Pop M,Duchene M,Ackers J,Tannich E,Leippe M,Hofer M,Bruchhaus I,Willhoeft U,Bhattacharya A,Chillingworth T,Churcher C,Hance Z,Harris B,Harris D,Jagels K,Moule S,Mungall K,Ormond D,Squares R,Whitehead S,Quail MA,Rabbinowitsch E,Norbertczak H,Price C,Wang Z,Guillén N,Gilchrist C,Stroup SE,Bhattacharya S,Lohia A,Foster PG,Sicheritz-Ponten T,Weber C,Singh U,Mukherjee C,El-Sayed NM,Petri WA Jr,Clark CG,Embley TM,Barrell B,Fraser CM,Hall N