ORF » Species Summary » Entamoeba histolytica HM-1:IMSS » EHI_013140 cDNA ORF clone

EHI_013140 cDNA ORF clone, Entamoeba histolytica HM-1:IMSS

The following EHI_013140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHI_013140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OEb01919 XM_644552.1
Latest version!		 Entamoeba histolytica HM-1:IMSS Ras guanine nucleotide exchange factor, putative (EHI_013140), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    EHI_013140 ( XM_644552.1 ) cDNA ORF clone, Entamoeba histolytica HM-1:IMSS -> XP_649644.1 Entamoeba histolytica HM-1:IMSS Ras guanine nucleotide exchange factor, putative (EHI_013140), partial mRNA.
    Price & Availability↑
    CloneID OEb01919
    Clone ID Related Accession (Same CDS sequence) XM_644552.1
    Accession Version XM_644552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1508774400000
    Organism Entamoeba histolytica HM-1:IMSS
    Product Ras guanine nucleotide exchange factor, putative
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001914883). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAACCAA CAATTGTTAT TGATCCATCA CATAAAAACA GATTATTCCA CAATGAAAGA 
    AGTACAACAT TTTCTACAAG ATCAAATGAT TCATTAGTAG GATATTTACA AAATCCTAGA 
    AATTATATGA CAGAACAACG AATACAAATA AGGTTAGCTC GTGAAGTTAA TATTTTGAAA 
    AATGGAATAG AAAGTAGTAA ATTTGTCATT GTTCATAATG AAGGAAATGG CACATCAAAA 
    AGGAGAAAAG AAATTAAAAA ACTTATACCT CCAGGATATA TTGAACGAGA TGTTAATGGT 
    AAAGTAATTA AAGGGGATGT TCGTGGACTT ATTGAATGTT TTTGTAGTGA AGAACAAAAT 
    GGAATGATGA AAGCATTTAT TTGGACATAC ACTGGTTTTT GTTCTACACA AGAAATGTTA 
    GAAGCTATGA TTGAAAGATA TGAAGTAGGA TGTAATGATG ATTCTAAACC AAGTTTATTA 
    ATTAGAGCAA GGGTAATTAG TGCAGTTAAA TATTGGATTG AAAATATTTG GGGAGTTATT 
    AAAGAAAATG AAAGTTCAGT TAATGATGTT GTTGGTAAGT TATTAAATTT ACTTAAAAGT 
    TATGGAATGG TACGAGAACA TCATTTAATT AAACAGTGTT ATCAAAGAAT TATGAATGAC 
    GTTGAAGTTG AATATGTTCA ATGTCAAAAG AACGTAGAAC AATCAATCCC TTCATTAAAT 
    AAAATTATTG GACGAACATT AAATCCAACT ATTTTTCAGT CAATACATCC AATAGAATAT 
    GCTCGTCAAA TAACTCTTAT TGTTTCAGAT ATGTTTAGAA GAATTAGGTT ATATGAAATG 
    TTAATGTGGT CAAATGGTGA GAAAGAGAGT TGTCAAAATA TTGTTTATGC ACAAACATTT 
    TTTAATGGAC TTCAAAATCA TTTCACTAGA ATGATTCTAA ATGAAACTGA AACAACTCAA 
    AGAGCAGCGA TTATAGAAAG ACTTGTTCGT GTAGCTGATT ATTGTTTTGA ATATCATAAT 
    TTTGATACAC TATTATGTAT TCTTCTTCTT TTTAATACAT CTTCCATTCA TCGTTTAAAG 
    AGGTCATTTG ATTCAATAAG TGGAGAGAGT AAAAAGATAT TAAATAAATT AATTCCTATT 
    GCTTGCCCAG ATAAAAATTG GAATGTTTTA AGAGAAGAAT CACGTCAGTT AGTTGGTAAA 
    CCAACAGTAC CATATATTGG CTTAATATTG AGTGATCTTA CTTTTACTAA TTTTGGTAAT 
    AAAACTAAAG AAAATGAAAT GGTTAATTTT GGTAAATGCA GACAAATTGC ACATATATTA 
    GAACAAGTTA TGTTTATGCA CCAACAAACA TATGCTCAAC TAATCATTAA AAATATAGAA 
    ATTCAAGCAG CAATAATCTC AGATTCAAGA TATGAAGTAA CTATGAGTGA TAAAGAAGCA 
    TTTGACTTAT CTTTAAAAAT TGAACCACGA ACTTCATCTA TTGTTCCATT ATCAGCAGGA 
    TGTACACGAC TAACTTTACA TTATCCAGGA AAAGAATTAG TAGTGGTTGC AGCTAAAGAC 
    GCAAATGTAT TAATTTCTCA AATGTTCTGG GATGCAATGG GTGTTGATGT TTCTAAATGC 
    CAAATATATA TTTTTTATAC TGGAAATAGT ATTAATGAAA CAATGAGTCC ATCTTCTCCT 
    GTTGGAAGAA TTAAGATTGC AGTAAAAGAG ACTGAGGTAA TTGTTACTGC TTTACAAAAA 
    GTGTTATATG TTAATTTAGT AATGGAGTAT AATAAAGAAG TATGTAAAAC AACGATTCCA 
    TTAGATCCAA CCCTTCCTTT AAATCAATTA CAACCAATTC TTCAAACATT TACAGTAATA 
    CCATCATATC CTATCTTACT TAAAGGAGAA AAAGTTATAG GTTGTATGTG CCCGTCATTG 
    TCATTAGATG AAATAGAATG GATTGATGGA GATACTATTT ATTTACTACC TCTTCGTGAA 
    TTAAAAAGTC AATTAGGAGA TTTATTAAGT GATAGACTTC GCTTTAAATC AGCACGGTTT 
    TACAATGAAT ATGTTTTTCA TTTAGATAAT GTTAATGCAA TAGCTATTGT AGTTGATGTA 
    ATGGTAATTA TTTATGTCGA AGAACATTTT AAGGCTATTT ATCCAATTGA ATTACTTTCT 
    ATAGAACTAA ACGTTTGGAA AAATACCATT TCTTTTAGGT TAGATGAAAA TTATATTGAC 
    AATAGATTAA TTTTAGCATT CACATCTCCA CTAACTTTAA AATGTGATTT AACAACAGCA 
    AGAGGATTTA TTAAAAGAAT TTGTCAAATT AGTAGGAAAC AACGATACCA ACGATTGTTT 
    GGAACGGACC GACATGATAT TGAATGTTCT AACACTGGAG CTCCAAAACA ATTTGAAAGA 
    TTAATTGAAG TTATCTATAA CTCTGAAGAA TTTACCAAAC AAGGATATTT TGATGTAAGG 
    AATGATGATT GTGTTGCTCA AATAAGAAAT TTTGAAGAAC ATGGAATTAT AAGAAGTCCT 
    ATTGCAGGAG GATTACTTGT TATGTATTTA CTATCATTAA ACGAACCAAT GATTCCAATT 
    GAACATACAA AACATTTTGT GTCATTTGAT TTCCTAGATA AAGAAGATCG TATTGAATTA 
    TTAAGAAGAG CATTTGATGA ATTAACAGAT ACAATGAGAC CTATTGTTAG TATAGTCATA 
    CAATTATTAT CATTACATTT TTCCTTCTCT AAAGATATAT TACCACAAGG ATTTGCTCCT 
    TTATTATTTT ATGGTTTAAC ACCAGGATTA TCAGAACGTA TTTTATCTTT TTTAATTCTA 
    AATTCAAGTA ATGTCATTAA TCAACAACCA AAAGTAGAGG TTGTACATGA ATTTGTATTG 
    TCTCTACCTT CTATTGATTT TCTCCCTACT TCTACTGTTC TTCAAATTAA TGCAGGGGTT 
    GCTGCTTCTT CAGAGGCATC ATCAGCAAAT GAACTAATAG CAAAAAAGAA TGCAAGAATT 
    ATTCCACATT TATTAAATGA TTCAACCCGT TCACTAATAG AAACACCAAG GAGGCACGTT 
    CAAAAGAGAC GTTTTGGTAA AACAGACTCT TTGAATCTAA AAGCAAAAAC ACAACTAACA 
    TTTGCAAATC TTAGTGATTT CCAAAAGAGT TCTCCAGTAA TGAGGTCTGT TATTTTTTCT 
    GTTGGTCCCC AACGAACACA ATTATCTCCT TCAAAATTAT GTCATTCTCC ATTAAACACA 
    CCTAACTCAG TTTCTTCATC TACTACCTTA ACACCTTCAA TTATATCTCC TCTCAATGAC 
    TCAAGAAATT CATTAGAAGT TCCTAACCAA AAAGTAGAAA GTAAATCAAT CACTCCTTCT 
    TCTAATGGTA GTAGTTCACA ACAACAAACT GGTGTTAAAC ACCAAGAACA TAAAATAGAG 
    ATTAATATTG ATAATAAAAA GACAATTTTC TCTAGTAAAT TTGGTCAAAT AAGACAATTT 
    TACGAATCTA AAGATTTAGC TGTCACTTCT CCTCGTATAA AATCACAAAC TATTCAAAGC 
    GAAAAAACTT CTCCAAAAAC TTCACCACGA ATCTCTCCAA GAACACCTCC AATAATAAAG 
    GACAAAGAAC AACAAGTTGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_649644.1
    CDS1..3627
    Translation

    Target ORF information:

    RefSeq Version XM_644552.1
    Organism Entamoeba histolytica HM-1:IMSS
    Definition Entamoeba histolytica HM-1:IMSS Ras guanine nucleotide exchange factor, putative (EHI_013140), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_644552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCAACCAA CAATTGTTAT TGATCCATCA CATAAAAACA GATTATTCCA CAATGAAAGA 
    AGTACAACAT TTTCTACAAG ATCAAATGAT TCATTAGTAG GATATTTACA AAATCCTAGA 
    AATTATATGA CAGAACAACG AATACAAATA AGGTTAGCTC GTGAAGTTAA TATTTTGAAA 
    AATGGAATAG AAAGTAGTAA ATTTGTCATT GTTCATAATG AAGGAAATGG CACATCAAAA 
    AGGAGAAAAG AAATTAAAAA ACTTATACCT CCAGGATATA TTGAACGAGA TGTTAATGGT 
    AAAGTAATTA AAGGGGATGT TCGTGGACTT ATTGAATGTT TTTGTAGTGA AGAACAAAAT 
    GGAATGATGA AAGCATTTAT TTGGACATAC ACTGGTTTTT GTTCTACACA AGAAATGTTA 
    GAAGCTATGA TTGAAAGATA TGAAGTAGGA TGTAATGATG ATTCTAAACC AAGTTTATTA 
    ATTAGAGCAA GGGTAATTAG TGCAGTTAAA TATTGGATTG AAAATATTTG GGGAGTTATT 
    AAAGAAAATG AAAGTTCAGT TAATGATGTT GTTGGTAAGT TATTAAATTT ACTTAAAAGT 
    TATGGAATGG TACGAGAACA TCATTTAATT AAACAGTGTT ATCAAAGAAT TATGAATGAC 
    GTTGAAGTTG AATATGTTCA ATGTCAAAAG AACGTAGAAC AATCAATCCC TTCATTAAAT 
    AAAATTATTG GACGAACATT AAATCCAACT ATTTTTCAGT CAATACATCC AATAGAATAT 
    GCTCGTCAAA TAACTCTTAT TGTTTCAGAT ATGTTTAGAA GAATTAGGTT ATATGAAATG 
    TTAATGTGGT CAAATGGTGA GAAAGAGAGT TGTCAAAATA TTGTTTATGC ACAAACATTT 
    TTTAATGGAC TTCAAAATCA TTTCACTAGA ATGATTCTAA ATGAAACTGA AACAACTCAA 
    AGAGCAGCGA TTATAGAAAG ACTTGTTCGT GTAGCTGATT ATTGTTTTGA ATATCATAAT 
    TTTGATACAC TATTATGTAT TCTTCTTCTT TTTAATACAT CTTCCATTCA TCGTTTAAAG 
    AGGTCATTTG ATTCAATAAG TGGAGAGAGT AAAAAGATAT TAAATAAATT AATTCCTATT 
    GCTTGCCCAG ATAAAAATTG GAATGTTTTA AGAGAAGAAT CACGTCAGTT AGTTGGTAAA 
    CCAACAGTAC CATATATTGG CTTAATATTG AGTGATCTTA CTTTTACTAA TTTTGGTAAT 
    AAAACTAAAG AAAATGAAAT GGTTAATTTT GGTAAATGCA GACAAATTGC ACATATATTA 
    GAACAAGTTA TGTTTATGCA CCAACAAACA TATGCTCAAC TAATCATTAA AAATATAGAA 
    ATTCAAGCAG CAATAATCTC AGATTCAAGA TATGAAGTAA CTATGAGTGA TAAAGAAGCA 
    TTTGACTTAT CTTTAAAAAT TGAACCACGA ACTTCATCTA TTGTTCCATT ATCAGCAGGA 
    TGTACACGAC TAACTTTACA TTATCCAGGA AAAGAATTAG TAGTGGTTGC AGCTAAAGAC 
    GCAAATGTAT TAATTTCTCA AATGTTCTGG GATGCAATGG GTGTTGATGT TTCTAAATGC 
    CAAATATATA TTTTTTATAC TGGAAATAGT ATTAATGAAA CAATGAGTCC ATCTTCTCCT 
    GTTGGAAGAA TTAAGATTGC AGTAAAAGAG ACTGAGGTAA TTGTTACTGC TTTACAAAAA 
    GTGTTATATG TTAATTTAGT AATGGAGTAT AATAAAGAAG TATGTAAAAC AACGATTCCA 
    TTAGATCCAA CCCTTCCTTT AAATCAATTA CAACCAATTC TTCAAACATT TACAGTAATA 
    CCATCATATC CTATCTTACT TAAAGGAGAA AAAGTTATAG GTTGTATGTG CCCGTCATTG 
    TCATTAGATG AAATAGAATG GATTGATGGA GATACTATTT ATTTACTACC TCTTCGTGAA 
    TTAAAAAGTC AATTAGGAGA TTTATTAAGT GATAGACTTC GCTTTAAATC AGCACGGTTT 
    TACAATGAAT ATGTTTTTCA TTTAGATAAT GTTAATGCAA TAGCTATTGT AGTTGATGTA 
    ATGGTAATTA TTTATGTCGA AGAACATTTT AAGGCTATTT ATCCAATTGA ATTACTTTCT 
    ATAGAACTAA ACGTTTGGAA AAATACCATT TCTTTTAGGT TAGATGAAAA TTATATTGAC 
    AATAGATTAA TTTTAGCATT CACATCTCCA CTAACTTTAA AATGTGATTT AACAACAGCA 
    AGAGGATTTA TTAAAAGAAT TTGTCAAATT AGTAGGAAAC AACGATACCA ACGATTGTTT 
    GGAACGGACC GACATGATAT TGAATGTTCT AACACTGGAG CTCCAAAACA ATTTGAAAGA 
    TTAATTGAAG TTATCTATAA CTCTGAAGAA TTTACCAAAC AAGGATATTT TGATGTAAGG 
    AATGATGATT GTGTTGCTCA AATAAGAAAT TTTGAAGAAC ATGGAATTAT AAGAAGTCCT 
    ATTGCAGGAG GATTACTTGT TATGTATTTA CTATCATTAA ACGAACCAAT GATTCCAATT 
    GAACATACAA AACATTTTGT GTCATTTGAT TTCCTAGATA AAGAAGATCG TATTGAATTA 
    TTAAGAAGAG CATTTGATGA ATTAACAGAT ACAATGAGAC CTATTGTTAG TATAGTCATA 
    CAATTATTAT CATTACATTT TTCCTTCTCT AAAGATATAT TACCACAAGG ATTTGCTCCT 
    TTATTATTTT ATGGTTTAAC ACCAGGATTA TCAGAACGTA TTTTATCTTT TTTAATTCTA 
    AATTCAAGTA ATGTCATTAA TCAACAACCA AAAGTAGAGG TTGTACATGA ATTTGTATTG 
    TCTCTACCTT CTATTGATTT TCTCCCTACT TCTACTGTTC TTCAAATTAA TGCAGGGGTT 
    GCTGCTTCTT CAGAGGCATC ATCAGCAAAT GAACTAATAG CAAAAAAGAA TGCAAGAATT 
    ATTCCACATT TATTAAATGA TTCAACCCGT TCACTAATAG AAACACCAAG GAGGCACGTT 
    CAAAAGAGAC GTTTTGGTAA AACAGACTCT TTGAATCTAA AAGCAAAAAC ACAACTAACA 
    TTTGCAAATC TTAGTGATTT CCAAAAGAGT TCTCCAGTAA TGAGGTCTGT TATTTTTTCT 
    GTTGGTCCCC AACGAACACA ATTATCTCCT TCAAAATTAT GTCATTCTCC ATTAAACACA 
    CCTAACTCAG TTTCTTCATC TACTACCTTA ACACCTTCAA TTATATCTCC TCTCAATGAC 
    TCAAGAAATT CATTAGAAGT TCCTAACCAA AAAGTAGAAA GTAAATCAAT CACTCCTTCT 
    TCTAATGGTA GTAGTTCACA ACAACAAACT GGTGTTAAAC ACCAAGAACA TAAAATAGAG 
    ATTAATATTG ATAATAAAAA GACAATTTTC TCTAGTAAAT TTGGTCAAAT AAGACAATTT 
    TACGAATCTA AAGATTTAGC TGTCACTTCT CCTCGTATAA AATCACAAAC TATTCAAAGC 
    GAAAAAACTT CTCCAAAAAC TTCACCACGA ATCTCTCCAA GAACACCTCC AATAATAAAG 
    GACAAAGAAC AACAAGTTGA AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the protist parasite Entamoeba histolytica.
    Nature433(7028)865-8(2005 Feb)
    Loftus B,Anderson I,Davies R,Alsmark UC,Samuelson J,Amedeo P,Roncaglia P,Berriman M,Hirt RP,Mann BJ,Nozaki T,Suh B,Pop M,Duchene M,Ackers J,Tannich E,Leippe M,Hofer M,Bruchhaus I,Willhoeft U,Bhattacharya A,Chillingworth T,Churcher C,Hance Z,Harris B,Harris D,Jagels K,Moule S,Mungall K,Ormond D,Squares R,Whitehead S,Quail MA,Rabbinowitsch E,Norbertczak H,Price C,Wang Z,Guillén N,Gilchrist C,Stroup SE,Bhattacharya S,Lohia A,Foster PG,Sicheritz-Ponten T,Weber C,Singh U,Mukherjee C,El-Sayed NM,Petri WA Jr,Clark CG,Embley TM,Barrell B,Fraser CM,Hall N


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