LOC6640653 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6640653
Entrez Gene ID	6640653
Full Name	spondin-1
Synonyms	GK21893,GK21893-PA
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_002063374.3	XP_002063410.2	spondin-1 isoform X1
XM_002063374.2	XP_002063410.1	uncharacterized protein
XM_023174887.1	XP_023030655.1	spondin-1 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6640653 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6640653 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODf02953	XM_002063374.2 Latest version!	Drosophila willistoni uncharacterized protein (Dwil\GK21893), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODf23364	XM_002063374.3 Latest version!	Drosophila willistoni spondin-1 (LOC6640653), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODf23365	XM_023174887.1 Latest version!	Drosophila willistoni spondin-1 (LOC6640653), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6640653 ( XM_002063374.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002063410.1 Drosophila willistoni uncharacterized protein (Dwil\GK21893), partial mRNA.

Price & Availability↑

CloneID	ODf02953
Clone ID Related Accession (Same CDS sequence)	XM_002063374.2
Accession Version	XM_002063374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032470). On Dec 21, 2015 this sequence version replaced XM_002063374.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGAATGATC TATTAATTGT CCTCATTCTA CTATTGGCAT TCACTTGGCC ATCTCTGGGA 
CTGATTTGCA ATCGAGTTCC TTTGGGTGCC ACTGCAGCCA AGTCGCCAGT TGATGACAAT 
TATGTCCTCT CAGTGGCTGG GACACAACAG ACCTATGTTC CGGGTCAGAG ATATAATGTA 
ACATTAAATG CATATTCTGG ACTGTCCTTC GTGAGTTTTA TGCTAGCTTT GGATGTAGAA 
AATGGTGACA GTGAGGATGA TGCCAATGCT ATGGGTACTT TTGAACTTGT CGACATCTCA 
GAGACGCGTT TCAGTCCACG GTGCGCCAAT TTGGTGGAGA ACACCAATAC GAATGTGAAG 
ACACGCGTTG ATGTCGTCTG GGTGGCTCCC TCAACGCCTG GCCAGGGCTG CGTATTGTTA 
CGGGCAACTG TAATGCAGCA TCGCGATGTG TGGTTTATGG ACGATGGATT TCTCACCAAG 
CGAATGTGTG AGGAAGAGGT GGATGACATT GATACACAGC CAAGTATTGT GGATCCATGT 
TGTGCTTGTG ATGAAGCGAA ATATGAGTTG ACTTTCGAGG GCAAATGGTC GCGTCACACA 
CATCCCAAGG ACTTTCCGGC AAACAGTTGG CGTACACGTT TTAGCGATAT TATAGGCGCC 
TCTCACACCA TTGACTATCG GTTTTGGCAG TATGGAGAAC TCGCCAGCGA GGGTTTGCGT 
GAGGTGGCCG AGCATGGGTC CACTCGAACT CTAGAGAGTG AGCTCAAGGA TCAAAGTGAA 
CATATTCGGA CTATCATCAA GGCACGCGGC ATAGCCTATC CCAATGTGAC GGGGAAAACA 
TTTGCTGTGT TCCGTGTGGA CTCCAATCAC CATTTAATCT CATTGGTTTC CATGGTGGAT 
CCCTCACCGG ATTGGATAGT GGGCGTATCG GGACTGGAAC TCTGTTTGCC CAATTGCTCG 
TGGGTGGAAA ATAAAGTGCA CAATTTATAT CCCTGGGATG CGGGCACGGA TAGTGGCCCA 
TCTTATATGT CCGCTGACCA GCCACAAGTG CCGCCAGATG TGGTGCGTCG TATCAAATCG 
AATTTTCCCA ATGATCCACG TTCACCTTTC TACGATCCCA CCGGAGCCCA GATGAAGCCC 
TTGGCCACTC TGCATCTCAA TCGACGACGT CTCTATGAGA AAAACTGCGA GTCAGTGGAT 
GCTGAGCAAC AGCAAGCCGA GTGCGAAACA AATGCTTGGA GTCGATGGGA TGAATGCAGT 
GCCAAATGTG GACCCGGCAA ACAATACAGA ACACGTGACT TCAAGAATCC CGCTCTGGCT 
TCGCGCAATC GATGCAACAA TGCTTTGCGA GAGGAGAAGA ACTGTATGGG TCGCAAATGT 
TCCAGCTTTA ATGAAGAAGC ACCTGAAGGT GCTCAAGCGG AAGAGGGAGC TCCTCCATCC 
GCTTCCGAAG ATCCCATTTG CCAGGTTAGT GAGTGGAGCG AATGGTCAAG CTGTACGGTG 
ACATGCGGCA CAGGTGAAAT GATCCGATCG CGTCATTACC TCAACAAAAA GGCCAAGAAG 
AAGTGCCAGA AGGTCAGCAA GGTACGTCTG CAGGAGACAA AAGTCTGCGA GGCAATGGAA 
TGTGGCGGAG AGATTGCCAA TGGCGAAGGC GGGGCAGAGG AAGGAGAAGA TGATAACCAG 
GCAGGGAATG GCGACGAAGA GGCACAGGCT GAAAGACGCT CCCTATTTCG GAACTTTGAG 
AGCTATAGTC AGCATAAAGA GGAATACATT CCAGCAGCAT GTGGTGTATC GCCTTGGTCT 
GACTTCTCGC CCTGTATGGG TCCCTGTGGC GGCTCTGGGC AGCGGCAACG TATTCGTAAA 
GTATGGAATA ATCACCAAGT TTATGGCATT GAGCATGATG GCAAAGGCAG CCACGACCCT 
TGCCGACATA TCAAAGTTCA GGAAGAGGTC AACTGTACTA ATCCCAGCTG CGATACCATC 
GTGCCCCTTC ATTGCTACGA ACAACTCACG GAAAGCCATT GCCGCGACAG CGACGTAACC 
AATTATTGGT ATTACGATCA CATCAGCGAT CAATGCGGCA TCTATTGGTC TGATCGTTGT 
GACACCAATC GCAACAAGTT CAAGTCGAAA GAGGAATGCG AAGACACCTG CCGTTTGCCC 
CGACACAAAC AGGAGCTCCA ATACGAGGGA GTCCATCATG CCGTCGATTG TATGGTATCC 
GATTGGGTAG CCCACAGCTG TAATGCCACC TGCGGCGAGG GAGTCCAGTT GAAGACACGT 
CGTGTCCTGC GCACTCCCAA GTACGGGGGA AAATCCTGTC CAAAGCATTT GGTAAGGCAC 
GATCGCTGCT ATCAGCGTTG CGACGACATG TATACGGTAA CTGGTGGCGG GGGCTATGGT 
GAGCGGCGTC ACATGACCTC CAAGATGCAG CCCATGGAGC CGCGAGACGA ATGCCGCTAC 
TCAGAATGGT CGGCATGGAC GCCATGTACG GCCAGTTGTG GGGATAATGC AGTAAGACAA 
CGTACAAGGA CACTTCTCAA TACGGATCTT AGCTACAAGT GCAAGGATCG TGTACGAATT 
GAAAAGTGCG TTATGATGCC CTGTCTAATG AGTAGCAACG ATGAGGCAGA CAAGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002063410.1
CDS	1..2640
Translation	MNDLLIVLILLLAFTWPSLGLICNRVPLGATAAKSPVDDNYVLSVAGTQQTYVPGQRYNVTLNAYSGLSFVSFMLALDVENGDSEDDANAMGTFELVDISETRFSPRCANLVENTNTNVKTRVDVVWVAPSTPGQGCVLLRATVMQHRDVWFMDDGFLTKRMCEEEVDDIDTQPSIVDPCCACDEAKYELTFEGKWSRHTHPKDFPANSWRTRFSDIIGASHTIDYRFWQYGELASEGLREVAEHGSTRTLESELKDQSEHIRTIIKARGIAYPNVTGKTFAVFRVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQPQVPPDVVRRIKSNFPNDPRSPFYDPTGAQMKPLATLHLNRRRLYEKNCESVDAEQQQAECETNAWSRWDECSAKCGPGKQYRTRDFKNPALASRNRCNNALREEKNCMGRKCSSFNEEAPEGAQAEEGAPPSASEDPICQVSEWSEWSSCTVTCGTGEMIRSRHYLNKKAKKKCQKVSKVRLQETKVCEAMECGGEIANGEGGAEEGEDDNQAGNGDEEAQAERRSLFRNFESYSQHKEEYIPAACGVSPWSDFSPCMGPCGGSGQRQRIRKVWNNHQVYGIEHDGKGSHDPCRHIKVQEEVNCTNPSCDTIVPLHCYEQLTESHCRDSDVTNYWYYDHISDQCGIYWSDRCDTNRNKFKSKEECEDTCRLPRHKQELQYEGVHHAVDCMVSDWVAHSCNATCGEGVQLKTRRVLRTPKYGGKSCPKHLVRHDRCYQRCDDMYTVTGGGGYGERRHMTSKMQPMEPRDECRYSEWSAWTPCTASCGDNAVRQRTRTLLNTDLSYKCKDRVRIEKCVMMPCLMSSNDEADKW

Target ORF information:

RefSeq Version	XM_002063374.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK21893), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002063374.2

ORF Insert Sequence:

ATGAATGATC TATTAATTGT CCTCATTCTA CTATTGGCAT TCACTTGGCC ATCTCTGGGA 
CTGATTTGCA ATCGAGTTCC TTTGGGTGCC ACTGCAGCCA AGTCGCCAGT TGATGACAAT 
TATGTCCTCT CAGTGGCTGG GACACAACAG ACCTATGTTC CGGGTCAGAG ATATAATGTA 
ACATTAAATG CATATTCTGG ACTGTCCTTC GTGAGTTTTA TGCTAGCTTT GGATGTAGAA 
AATGGTGACA GTGAGGATGA TGCCAATGCT ATGGGTACTT TTGAACTTGT CGACATCTCA 
GAGACGCGTT TCAGTCCACG GTGCGCCAAT TTGGTGGAGA ACACCAATAC GAATGTGAAG 
ACACGCGTTG ATGTCGTCTG GGTGGCTCCC TCAACGCCTG GCCAGGGCTG CGTATTGTTA 
CGGGCAACTG TAATGCAGCA TCGCGATGTG TGGTTTATGG ACGATGGATT TCTCACCAAG 
CGAATGTGTG AGGAAGAGGT GGATGACATT GATACACAGC CAAGTATTGT GGATCCATGT 
TGTGCTTGTG ATGAAGCGAA ATATGAGTTG ACTTTCGAGG GCAAATGGTC GCGTCACACA 
CATCCCAAGG ACTTTCCGGC AAACAGTTGG CGTACACGTT TTAGCGATAT TATAGGCGCC 
TCTCACACCA TTGACTATCG GTTTTGGCAG TATGGAGAAC TCGCCAGCGA GGGTTTGCGT 
GAGGTGGCCG AGCATGGGTC CACTCGAACT CTAGAGAGTG AGCTCAAGGA TCAAAGTGAA 
CATATTCGGA CTATCATCAA GGCACGCGGC ATAGCCTATC CCAATGTGAC GGGGAAAACA 
TTTGCTGTGT TCCGTGTGGA CTCCAATCAC CATTTAATCT CATTGGTTTC CATGGTGGAT 
CCCTCACCGG ATTGGATAGT GGGCGTATCG GGACTGGAAC TCTGTTTGCC CAATTGCTCG 
TGGGTGGAAA ATAAAGTGCA CAATTTATAT CCCTGGGATG CGGGCACGGA TAGTGGCCCA 
TCTTATATGT CCGCTGACCA GCCACAAGTG CCGCCAGATG TGGTGCGTCG TATCAAATCG 
AATTTTCCCA ATGATCCACG TTCACCTTTC TACGATCCCA CCGGAGCCCA GATGAAGCCC 
TTGGCCACTC TGCATCTCAA TCGACGACGT CTCTATGAGA AAAACTGCGA GTCAGTGGAT 
GCTGAGCAAC AGCAAGCCGA GTGCGAAACA AATGCTTGGA GTCGATGGGA TGAATGCAGT 
GCCAAATGTG GACCCGGCAA ACAATACAGA ACACGTGACT TCAAGAATCC CGCTCTGGCT 
TCGCGCAATC GATGCAACAA TGCTTTGCGA GAGGAGAAGA ACTGTATGGG TCGCAAATGT 
TCCAGCTTTA ATGAAGAAGC ACCTGAAGGT GCTCAAGCGG AAGAGGGAGC TCCTCCATCC 
GCTTCCGAAG ATCCCATTTG CCAGGTTAGT GAGTGGAGCG AATGGTCAAG CTGTACGGTG 
ACATGCGGCA CAGGTGAAAT GATCCGATCG CGTCATTACC TCAACAAAAA GGCCAAGAAG 
AAGTGCCAGA AGGTCAGCAA GGTACGTCTG CAGGAGACAA AAGTCTGCGA GGCAATGGAA 
TGTGGCGGAG AGATTGCCAA TGGCGAAGGC GGGGCAGAGG AAGGAGAAGA TGATAACCAG 
GCAGGGAATG GCGACGAAGA GGCACAGGCT GAAAGACGCT CCCTATTTCG GAACTTTGAG 
AGCTATAGTC AGCATAAAGA GGAATACATT CCAGCAGCAT GTGGTGTATC GCCTTGGTCT 
GACTTCTCGC CCTGTATGGG TCCCTGTGGC GGCTCTGGGC AGCGGCAACG TATTCGTAAA 
GTATGGAATA ATCACCAAGT TTATGGCATT GAGCATGATG GCAAAGGCAG CCACGACCCT 
TGCCGACATA TCAAAGTTCA GGAAGAGGTC AACTGTACTA ATCCCAGCTG CGATACCATC 
GTGCCCCTTC ATTGCTACGA ACAACTCACG GAAAGCCATT GCCGCGACAG CGACGTAACC 
AATTATTGGT ATTACGATCA CATCAGCGAT CAATGCGGCA TCTATTGGTC TGATCGTTGT 
GACACCAATC GCAACAAGTT CAAGTCGAAA GAGGAATGCG AAGACACCTG CCGTTTGCCC 
CGACACAAAC AGGAGCTCCA ATACGAGGGA GTCCATCATG CCGTCGATTG TATGGTATCC 
GATTGGGTAG CCCACAGCTG TAATGCCACC TGCGGCGAGG GAGTCCAGTT GAAGACACGT 
CGTGTCCTGC GCACTCCCAA GTACGGGGGA AAATCCTGTC CAAAGCATTT GGTAAGGCAC 
GATCGCTGCT ATCAGCGTTG CGACGACATG TATACGGTAA CTGGTGGCGG GGGCTATGGT 
GAGCGGCGTC ACATGACCTC CAAGATGCAG CCCATGGAGC CGCGAGACGA ATGCCGCTAC 
TCAGAATGGT CGGCATGGAC GCCATGTACG GCCAGTTGTG GGGATAATGC AGTAAGACAA 
CGTACAAGGA CACTTCTCAA TACGGATCTT AGCTACAAGT GCAAGGATCG TGTACGAATT 
GAAAAGTGCG TTATGATGCC CTGTCTAATG AGTAGCAACG ATGAGGCAGA CAAGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6640653 ( XM_002063374.3 ) cDNA ORF clone, Drosophila willistoni -> XP_002063410.2 Drosophila willistoni spondin-1 (LOC6640653), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODf23364
Clone ID Related Accession (Same CDS sequence)	XM_002063374.3
Accession Version	XM_002063374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	spondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032470.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 4, 2017 this sequence version replaced XM_002063374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTAG TGGAAAGATT CCAAATGAAT GATCTATTAA TTGTCCTCAT TCTACTATTG 
GCATTCACTT GGCCATCTCT GGGACTGATT TGCAATCGAG TTCCTTTGGG TGCCACTGCA 
GCCAAGTCGC CAGTTGATGA CAATTATGTC CTCTCAGTGG CTGGGACACA ACAGACCTAT 
GTTCCGGGTC AGAGATATAA TGTAACATTA AATGCATATT CTGGACTGTC CTTCGTGAGT 
TTTATGCTAG CTTTGGATGT AGAAAATGGT GACAGTGAGG ATGATGCCAA TGCTATGGGT 
ACTTTTGAAC TTGTCGACAT CTCAGAGACG CGTTTCAGTC CACGGTGCGC CAATTTGGTG 
GAGAACACCA ATACGAATGT GAAGACACGC GTTGATGTCG TCTGGGTGGC TCCCTCAACG 
CCTGGCCAGG GCTGCGTATT GTTACGGGCA ACTGTAATGC AGCATCGCGA TGTGTGGTTT 
ATGGACGATG GATTTCTCAC CAAGCGAATG TGTGAGGAAG AGGTGGATGA CATTGATACA 
CAGCCAAGTA TTGTGGATCC ATGTTGTGCT TGTGATGAAG CGAAATATGA GTTGACTTTC 
GAGGGCAAAT GGTCGCGTCA CACACATCCC AAGGACTTTC CGGCAAACAG TTGGCGTACA 
CGTTTTAGCG ATATTATAGG CGCCTCTCAC ACCATTGACT ATCGGTTTTG GCAGTATGGA 
GAACTCGCCA GCGAGGGTTT GCGTGAGGTG GCCGAGCATG GGTCCACTCG AACTCTAGAG 
AGTGAGCTCA AGGATCAAAG TGAACATATT CGGACTATCA TCAAGGCACG CGGCATAGCC 
TATCCCAATG TGACGGGGAA AACATTTGCT GTGTTCCGTG TGGACTCCAA TCACCATTTA 
ATCTCATTGG TTTCCATGGT GGATCCCTCA CCGGATTGGA TAGTGGGCGT ATCGGGACTG 
GAACTCTGTT TGCCCAATTG CTCGTGGGTG GAAAATAAAG TGCACAATTT ATATCCCTGG 
GATGCGGGCA CGGATAGTGG CCCATCTTAT ATGTCCGCTG ACCAGCCACA AGTGCCGCCA 
GATGTGGTGC GTCGTATCAA ATCGAATTTT CCCAATGATC CACGTTCACC TTTCTACGAT 
CCCACCGGAG CCCAGATGAA GCCCTTGGCC ACTCTGCATC TCAATCGACG ACGTCTCTAT 
GAGAAAAACT GCGAGTCAGT GGATGCTGAG CAACAGCAAG CCGAGTGCGA AACAAATGCT 
TGGAGTCGAT GGGATGAATG CAGTGCCAAA TGTGGACCCG GCAAACAATA CAGAACACGT 
GACTTCAAGA ATCCCGCTCT GGCTTCGCGC AATCGATGCA ACAATGCTTT GCGAGAGGAG 
AAGAACTGTA TGGGTCGCAA ATGTTCCAGC TTTAATGAAG AAGCACCTGA AGGTGCTCAA 
GCGGAAGAGG GAGCTCCTCC ATCCGCTTCC GAAGATCCCA TTTGCCAGGT TAGTGAGTGG 
AGCGAATGGT CAAGCTGTAC GGTGACATGC GGCACAGGTG AAATGATCCG ATCGCGTCAT 
TACCTCAACA AAAAGGCCAA GAAGAAGTGC CAGAAGGTCA GCAAGGTACG TCTGCAGGAG 
ACAAAAGTCT GCGAGGCAAT GGAATGTGGC GGAGAGATTG CCAATGGCGA AGGCGGGGCA 
GAGGAAGGAG AAGATGATAA CCAGGCAGGG AATGGCGACG AAGAGGCACA GGCTGAAAGA 
CGCTCCCTAT TTCGGAACTT TGAGAGCTAT AGTCAGCATA AAGAGGAATA CATTCCAGCA 
GCATGTGGTG TATCGCCTTG GTCTGACTTC TCGCCCTGTA TGGGTCCCTG TGGCGGCTCT 
GGGCAGCGGC AACGTATTCG TAAAGTATGG AATAATCACC AAGTTTATGG CATTGAGCAT 
GATGGCAAAG GCAGCCACGA CCCTTGCCGA CATATCAAAG TTCAGGAAGA GGTCAACTGT 
ACTAATCCCA GCTGCGATAC CATCGTGCCC CTTCATTGCT ACGAACAACT CACGGAAAGC 
CATTGCCGCG ACAGCGACGT AACCAATTAT TGGTATTACG ATCACATCAG CGATCAATGC 
GGCATCTATT GGTCTGATCG TTGTGACACC AATCGCAACA AGTTCAAGTC GAAAGAGGAA 
TGCGAAGACA CCTGCCGTTT GCCCCGACAC AAACAGGAGC TCCAATACGA GGGAGTCCAT 
CATGCCGTCG ATTGTATGGT ATCCGATTGG GTAGCCCACA GCTGTAATGC CACCTGCGGC 
GAGGGAGTCC AGTTGAAGAC ACGTCGTGTC CTGCGCACTC CCAAGTACGG GGGAAAATCC 
TGTCCAAAGC ATTTGGTAAG GCACGATCGC TGCTATCAGC GTTGCGACGA CATGTATACG 
GTAACTGGTG GCGGGGGCTA TGGTGAGCGG CGTCACATGA CCTCCAAGAT GCAGCCCATG 
GAGCCGCGAG ACGAATGCCG CTACTCAGAA TGGTCGGCAT GGACGCCATG TACGGCCAGT 
TGTGGGGATA ATGCAGTAAG ACAACGTACA AGGACACTTC TCAATACGGA TCTTAGCTAC 
AAGTGCAAGG ATCGTGTACG AATTGAAAAG TGCGTTATGA TGCCCTGTCT AATGAGTAGC 
AACGATGAGG CAGACAAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002063410.2
CDS	16..2679
Translation	MKLVERFQMNDLLIVLILLLAFTWPSLGLICNRVPLGATAAKSPVDDNYVLSVAGTQQTYVPGQRYNVTLNAYSGLSFVSFMLALDVENGDSEDDANAMGTFELVDISETRFSPRCANLVENTNTNVKTRVDVVWVAPSTPGQGCVLLRATVMQHRDVWFMDDGFLTKRMCEEEVDDIDTQPSIVDPCCACDEAKYELTFEGKWSRHTHPKDFPANSWRTRFSDIIGASHTIDYRFWQYGELASEGLREVAEHGSTRTLESELKDQSEHIRTIIKARGIAYPNVTGKTFAVFRVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQPQVPPDVVRRIKSNFPNDPRSPFYDPTGAQMKPLATLHLNRRRLYEKNCESVDAEQQQAECETNAWSRWDECSAKCGPGKQYRTRDFKNPALASRNRCNNALREEKNCMGRKCSSFNEEAPEGAQAEEGAPPSASEDPICQVSEWSEWSSCTVTCGTGEMIRSRHYLNKKAKKKCQKVSKVRLQETKVCEAMECGGEIANGEGGAEEGEDDNQAGNGDEEAQAERRSLFRNFESYSQHKEEYIPAACGVSPWSDFSPCMGPCGGSGQRQRIRKVWNNHQVYGIEHDGKGSHDPCRHIKVQEEVNCTNPSCDTIVPLHCYEQLTESHCRDSDVTNYWYYDHISDQCGIYWSDRCDTNRNKFKSKEECEDTCRLPRHKQELQYEGVHHAVDCMVSDWVAHSCNATCGEGVQLKTRRVLRTPKYGGKSCPKHLVRHDRCYQRCDDMYTVTGGGGYGERRHMTSKMQPMEPRDECRYSEWSAWTPCTASCGDNAVRQRTRTLLNTDLSYKCKDRVRIEKCVMMPCLMSSNDEADKW

Target ORF information:

RefSeq Version	XM_002063374.3
Organism	Drosophila willistoni
Definition	Drosophila willistoni spondin-1 (LOC6640653), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002063374.3

ORF Insert Sequence:

ATGAAATTAG TGGAAAGATT CCAAATGAAT GATCTATTAA TTGTCCTCAT TCTACTATTG 
GCATTCACTT GGCCATCTCT GGGACTGATT TGCAATCGAG TTCCTTTGGG TGCCACTGCA 
GCCAAGTCGC CAGTTGATGA CAATTATGTC CTCTCAGTGG CTGGGACACA ACAGACCTAT 
GTTCCGGGTC AGAGATATAA TGTAACATTA AATGCATATT CTGGACTGTC CTTCGTGAGT 
TTTATGCTAG CTTTGGATGT AGAAAATGGT GACAGTGAGG ATGATGCCAA TGCTATGGGT 
ACTTTTGAAC TTGTCGACAT CTCAGAGACG CGTTTCAGTC CACGGTGCGC CAATTTGGTG 
GAGAACACCA ATACGAATGT GAAGACACGC GTTGATGTCG TCTGGGTGGC TCCCTCAACG 
CCTGGCCAGG GCTGCGTATT GTTACGGGCA ACTGTAATGC AGCATCGCGA TGTGTGGTTT 
ATGGACGATG GATTTCTCAC CAAGCGAATG TGTGAGGAAG AGGTGGATGA CATTGATACA 
CAGCCAAGTA TTGTGGATCC ATGTTGTGCT TGTGATGAAG CGAAATATGA GTTGACTTTC 
GAGGGCAAAT GGTCGCGTCA CACACATCCC AAGGACTTTC CGGCAAACAG TTGGCGTACA 
CGTTTTAGCG ATATTATAGG CGCCTCTCAC ACCATTGACT ATCGGTTTTG GCAGTATGGA 
GAACTCGCCA GCGAGGGTTT GCGTGAGGTG GCCGAGCATG GGTCCACTCG AACTCTAGAG 
AGTGAGCTCA AGGATCAAAG TGAACATATT CGGACTATCA TCAAGGCACG CGGCATAGCC 
TATCCCAATG TGACGGGGAA AACATTTGCT GTGTTCCGTG TGGACTCCAA TCACCATTTA 
ATCTCATTGG TTTCCATGGT GGATCCCTCA CCGGATTGGA TAGTGGGCGT ATCGGGACTG 
GAACTCTGTT TGCCCAATTG CTCGTGGGTG GAAAATAAAG TGCACAATTT ATATCCCTGG 
GATGCGGGCA CGGATAGTGG CCCATCTTAT ATGTCCGCTG ACCAGCCACA AGTGCCGCCA 
GATGTGGTGC GTCGTATCAA ATCGAATTTT CCCAATGATC CACGTTCACC TTTCTACGAT 
CCCACCGGAG CCCAGATGAA GCCCTTGGCC ACTCTGCATC TCAATCGACG ACGTCTCTAT 
GAGAAAAACT GCGAGTCAGT GGATGCTGAG CAACAGCAAG CCGAGTGCGA AACAAATGCT 
TGGAGTCGAT GGGATGAATG CAGTGCCAAA TGTGGACCCG GCAAACAATA CAGAACACGT 
GACTTCAAGA ATCCCGCTCT GGCTTCGCGC AATCGATGCA ACAATGCTTT GCGAGAGGAG 
AAGAACTGTA TGGGTCGCAA ATGTTCCAGC TTTAATGAAG AAGCACCTGA AGGTGCTCAA 
GCGGAAGAGG GAGCTCCTCC ATCCGCTTCC GAAGATCCCA TTTGCCAGGT TAGTGAGTGG 
AGCGAATGGT CAAGCTGTAC GGTGACATGC GGCACAGGTG AAATGATCCG ATCGCGTCAT 
TACCTCAACA AAAAGGCCAA GAAGAAGTGC CAGAAGGTCA GCAAGGTACG TCTGCAGGAG 
ACAAAAGTCT GCGAGGCAAT GGAATGTGGC GGAGAGATTG CCAATGGCGA AGGCGGGGCA 
GAGGAAGGAG AAGATGATAA CCAGGCAGGG AATGGCGACG AAGAGGCACA GGCTGAAAGA 
CGCTCCCTAT TTCGGAACTT TGAGAGCTAT AGTCAGCATA AAGAGGAATA CATTCCAGCA 
GCATGTGGTG TATCGCCTTG GTCTGACTTC TCGCCCTGTA TGGGTCCCTG TGGCGGCTCT 
GGGCAGCGGC AACGTATTCG TAAAGTATGG AATAATCACC AAGTTTATGG CATTGAGCAT 
GATGGCAAAG GCAGCCACGA CCCTTGCCGA CATATCAAAG TTCAGGAAGA GGTCAACTGT 
ACTAATCCCA GCTGCGATAC CATCGTGCCC CTTCATTGCT ACGAACAACT CACGGAAAGC 
CATTGCCGCG ACAGCGACGT AACCAATTAT TGGTATTACG ATCACATCAG CGATCAATGC 
GGCATCTATT GGTCTGATCG TTGTGACACC AATCGCAACA AGTTCAAGTC GAAAGAGGAA 
TGCGAAGACA CCTGCCGTTT GCCCCGACAC AAACAGGAGC TCCAATACGA GGGAGTCCAT 
CATGCCGTCG ATTGTATGGT ATCCGATTGG GTAGCCCACA GCTGTAATGC CACCTGCGGC 
GAGGGAGTCC AGTTGAAGAC ACGTCGTGTC CTGCGCACTC CCAAGTACGG GGGAAAATCC 
TGTCCAAAGC ATTTGGTAAG GCACGATCGC TGCTATCAGC GTTGCGACGA CATGTATACG 
GTAACTGGTG GCGGGGGCTA TGGTGAGCGG CGTCACATGA CCTCCAAGAT GCAGCCCATG 
GAGCCGCGAG ACGAATGCCG CTACTCAGAA TGGTCGGCAT GGACGCCATG TACGGCCAGT 
TGTGGGGATA ATGCAGTAAG ACAACGTACA AGGACACTTC TCAATACGGA TCTTAGCTAC 
AAGTGCAAGG ATCGTGTACG AATTGAAAAG TGCGTTATGA TGCCCTGTCT AATGAGTAGC 
AACGATGAGG CAGACAAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6640653 ( XM_023174887.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023030655.1 Drosophila willistoni spondin-1 (LOC6640653), transcript variant X2, mRNA.

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CloneID	ODf23365
Clone ID Related Accession (Same CDS sequence)	XM_023174887.1
Accession Version	XM_023174887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	spondin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032470.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAT TCCAAATGAA TGATCTATTA ATTGTCCTCA TTCTACTATT GGCATTCACT 
TGGCCATCTC TGGGACTGAT TTGCAATCGA GTTCCTTTGG GTGCCACTGC AGCCAAGTCG 
CCAGTTGATG ACAATTATGT CCTCTCAGTG GCTGGGACAC AACAGACCTA TGTTCCGGGT 
CAGAGATATA ATGTAACATT AAATGCATAT TCTGGACTGT CCTTCGTGAG TTTTATGCTA 
GCTTTGGATG TAGAAAATGG TGACAGTGAG GATGATGCCA ATGCTATGGG TACTTTTGAA 
CTTGTCGACA TCTCAGAGAC GCGTTTCAGT CCACGGTGCG CCAATTTGGT GGAGAACACC 
AATACGAATG TGAAGACACG CGTTGATGTC GTCTGGGTGG CTCCCTCAAC GCCTGGCCAG 
GGCTGCGTAT TGTTACGGGC AACTGTAATG CAGCATCGCG ATGTGTGGTT TATGGACGAT 
GGATTTCTCA CCAAGCGAAT GTGTGAGGAA GAGGTGGATG ACATTGATAC ACAGCCAAGT 
ATTGTGGATC CATGTTGTGC TTGTGATGAA GCGAAATATG AGTTGACTTT CGAGGGCAAA 
TGGTCGCGTC ACACACATCC CAAGGACTTT CCGGCAAACA GTTGGCGTAC ACGTTTTAGC 
GATATTATAG GCGCCTCTCA CACCATTGAC TATCGGTTTT GGCAGTATGG AGAACTCGCC 
AGCGAGGGTT TGCGTGAGGT GGCCGAGCAT GGGTCCACTC GAACTCTAGA GAGTGAGCTC 
AAGGATCAAA GTGAACATAT TCGGACTATC ATCAAGGCAC GCGGCATAGC CTATCCCAAT 
GTGACGGGGA AAACATTTGC TGTGTTCCGT GTGGACTCCA ATCACCATTT AATCTCATTG 
GTTTCCATGG TGGATCCCTC ACCGGATTGG ATAGTGGGCG TATCGGGACT GGAACTCTGT 
TTGCCCAATT GCTCGTGGGT GGAAAATAAA GTGCACAATT TATATCCCTG GGATGCGGGC 
ACGGATAGTG GCCCATCTTA TATGTCCGCT GACCAGCCAC AAGTGCCGCC AGATGTGGTG 
CGTCGTATCA AATCGAATTT TCCCAATGAT CCACGTTCAC CTTTCTACGA TCCCACCGGA 
GCCCAGATGA AGCCCTTGGC CACTCTGCAT CTCAATCGAC GACGTCTCTA TGAGAAAAAC 
TGCGAGTCAG TGGATGCTGA GCAACAGCAA GCCGAGTGCG AAACAAATGC TTGGAGTCGA 
TGGGATGAAT GCAGTGCCAA ATGTGGACCC GGCAAACAAT ACAGAACACG TGACTTCAAG 
AATCCCGCTC TGGCTTCGCG CAATCGATGC AACAATGCTT TGCGAGAGGA GAAGAACTGT 
ATGGGTCGCA AATGTTCCAG CTTTAATGAA GAAGCACCTG AAGGTGCTCA AGCGGAAGAG 
GGAGCTCCTC CATCCGCTTC CGAAGATCCC ATTTGCCAGG TTAGTGAGTG GAGCGAATGG 
TCAAGCTGTA CGGTGACATG CGGCACAGGT GAAATGATCC GATCGCGTCA TTACCTCAAC 
AAAAAGGCCA AGAAGAAGTG CCAGAAGGTC AGCAAGGTAC GTCTGCAGGA GACAAAAGTC 
TGCGAGGCAA TGGAATGTGG CGGAGAGATT GCCAATGGCG AAGGCGGGGC AGAGGAAGGA 
GAAGATGATA ACCAGGCAGG GAATGGCGAC GAAGAGGCAC AGGCTGAAAG ACGCTCCCTA 
TTTCGGAACT TTGAGAGCTA TAGTCAGCAT AAAGAGGAAT ACATTCCAGC AGCATGTGGT 
GTATCGCCTT GGTCTGACTT CTCGCCCTGT ATGGGTCCCT GTGGCGGCTC TGGGCAGCGG 
CAACGTATTC GTAAAGTATG GAATAATCAC CAAGTTTATG GCATTGAGCA TGATGGCAAA 
GGCAGCCACG ACCCTTGCCG ACATATCAAA GTTCAGGAAG AGGTCAACTG TACTAATCCC 
AGCTGCGATA CCATCGTGCC CCTTCATTGC TACGAACAAC TCACGGAAAG CCATTGCCGC 
GACAGCGACG TAACCAATTA TTGGTATTAC GATCACATCA GCGATCAATG CGGCATCTAT 
TGGTCTGATC GTTGTGACAC CAATCGCAAC AAGTTCAAGT CGAAAGAGGA ATGCGAAGAC 
ACCTGCCGTT TGCCCCGACA CAAACAGGAG CTCCAATACG AGGGAGTCCA TCATGCCGTC 
GATTGTATGG TATCCGATTG GGTAGCCCAC AGCTGTAATG CCACCTGCGG CGAGGGAGTC 
CAGTTGAAGA CACGTCGTGT CCTGCGCACT CCCAAGTACG GGGGAAAATC CTGTCCAAAG 
CATTTGGTAA GGCACGATCG CTGCTATCAG CGTTGCGACG ACATGTATAC GGTAACTGGT 
GGCGGGGGCT ATGGTGAGCG GCGTCACATG ACCTCCAAGA TGCAGCCCAT GGAGCCGCGA 
GACGAATGCC GCTACTCAGA ATGGTCGGCA TGGACGCCAT GTACGGCCAG TTGTGGGGAT 
AATGCAGTAA GACAACGTAC AAGGACACTT CTCAATACGG ATCTTAGCTA CAAGTGCAAG 
GATCGTGTAC GAATTGAAAA GTGCGTTATG ATGCCCTGTC TAATGAGTAG CAACGATGAG 
GCAGACAAGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023030655.1
CDS	16..2670
Translation	MSVFQMNDLLIVLILLLAFTWPSLGLICNRVPLGATAAKSPVDDNYVLSVAGTQQTYVPGQRYNVTLNAYSGLSFVSFMLALDVENGDSEDDANAMGTFELVDISETRFSPRCANLVENTNTNVKTRVDVVWVAPSTPGQGCVLLRATVMQHRDVWFMDDGFLTKRMCEEEVDDIDTQPSIVDPCCACDEAKYELTFEGKWSRHTHPKDFPANSWRTRFSDIIGASHTIDYRFWQYGELASEGLREVAEHGSTRTLESELKDQSEHIRTIIKARGIAYPNVTGKTFAVFRVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQPQVPPDVVRRIKSNFPNDPRSPFYDPTGAQMKPLATLHLNRRRLYEKNCESVDAEQQQAECETNAWSRWDECSAKCGPGKQYRTRDFKNPALASRNRCNNALREEKNCMGRKCSSFNEEAPEGAQAEEGAPPSASEDPICQVSEWSEWSSCTVTCGTGEMIRSRHYLNKKAKKKCQKVSKVRLQETKVCEAMECGGEIANGEGGAEEGEDDNQAGNGDEEAQAERRSLFRNFESYSQHKEEYIPAACGVSPWSDFSPCMGPCGGSGQRQRIRKVWNNHQVYGIEHDGKGSHDPCRHIKVQEEVNCTNPSCDTIVPLHCYEQLTESHCRDSDVTNYWYYDHISDQCGIYWSDRCDTNRNKFKSKEECEDTCRLPRHKQELQYEGVHHAVDCMVSDWVAHSCNATCGEGVQLKTRRVLRTPKYGGKSCPKHLVRHDRCYQRCDDMYTVTGGGGYGERRHMTSKMQPMEPRDECRYSEWSAWTPCTASCGDNAVRQRTRTLLNTDLSYKCKDRVRIEKCVMMPCLMSSNDEADKW

Target ORF information:

RefSeq Version	XM_023174887.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni spondin-1 (LOC6640653), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023174887.1

ORF Insert Sequence:

ATGTCTGTAT TCCAAATGAA TGATCTATTA ATTGTCCTCA TTCTACTATT GGCATTCACT 
TGGCCATCTC TGGGACTGAT TTGCAATCGA GTTCCTTTGG GTGCCACTGC AGCCAAGTCG 
CCAGTTGATG ACAATTATGT CCTCTCAGTG GCTGGGACAC AACAGACCTA TGTTCCGGGT 
CAGAGATATA ATGTAACATT AAATGCATAT TCTGGACTGT CCTTCGTGAG TTTTATGCTA 
GCTTTGGATG TAGAAAATGG TGACAGTGAG GATGATGCCA ATGCTATGGG TACTTTTGAA 
CTTGTCGACA TCTCAGAGAC GCGTTTCAGT CCACGGTGCG CCAATTTGGT GGAGAACACC 
AATACGAATG TGAAGACACG CGTTGATGTC GTCTGGGTGG CTCCCTCAAC GCCTGGCCAG 
GGCTGCGTAT TGTTACGGGC AACTGTAATG CAGCATCGCG ATGTGTGGTT TATGGACGAT 
GGATTTCTCA CCAAGCGAAT GTGTGAGGAA GAGGTGGATG ACATTGATAC ACAGCCAAGT 
ATTGTGGATC CATGTTGTGC TTGTGATGAA GCGAAATATG AGTTGACTTT CGAGGGCAAA 
TGGTCGCGTC ACACACATCC CAAGGACTTT CCGGCAAACA GTTGGCGTAC ACGTTTTAGC 
GATATTATAG GCGCCTCTCA CACCATTGAC TATCGGTTTT GGCAGTATGG AGAACTCGCC 
AGCGAGGGTT TGCGTGAGGT GGCCGAGCAT GGGTCCACTC GAACTCTAGA GAGTGAGCTC 
AAGGATCAAA GTGAACATAT TCGGACTATC ATCAAGGCAC GCGGCATAGC CTATCCCAAT 
GTGACGGGGA AAACATTTGC TGTGTTCCGT GTGGACTCCA ATCACCATTT AATCTCATTG 
GTTTCCATGG TGGATCCCTC ACCGGATTGG ATAGTGGGCG TATCGGGACT GGAACTCTGT 
TTGCCCAATT GCTCGTGGGT GGAAAATAAA GTGCACAATT TATATCCCTG GGATGCGGGC 
ACGGATAGTG GCCCATCTTA TATGTCCGCT GACCAGCCAC AAGTGCCGCC AGATGTGGTG 
CGTCGTATCA AATCGAATTT TCCCAATGAT CCACGTTCAC CTTTCTACGA TCCCACCGGA 
GCCCAGATGA AGCCCTTGGC CACTCTGCAT CTCAATCGAC GACGTCTCTA TGAGAAAAAC 
TGCGAGTCAG TGGATGCTGA GCAACAGCAA GCCGAGTGCG AAACAAATGC TTGGAGTCGA 
TGGGATGAAT GCAGTGCCAA ATGTGGACCC GGCAAACAAT ACAGAACACG TGACTTCAAG 
AATCCCGCTC TGGCTTCGCG CAATCGATGC AACAATGCTT TGCGAGAGGA GAAGAACTGT 
ATGGGTCGCA AATGTTCCAG CTTTAATGAA GAAGCACCTG AAGGTGCTCA AGCGGAAGAG 
GGAGCTCCTC CATCCGCTTC CGAAGATCCC ATTTGCCAGG TTAGTGAGTG GAGCGAATGG 
TCAAGCTGTA CGGTGACATG CGGCACAGGT GAAATGATCC GATCGCGTCA TTACCTCAAC 
AAAAAGGCCA AGAAGAAGTG CCAGAAGGTC AGCAAGGTAC GTCTGCAGGA GACAAAAGTC 
TGCGAGGCAA TGGAATGTGG CGGAGAGATT GCCAATGGCG AAGGCGGGGC AGAGGAAGGA 
GAAGATGATA ACCAGGCAGG GAATGGCGAC GAAGAGGCAC AGGCTGAAAG ACGCTCCCTA 
TTTCGGAACT TTGAGAGCTA TAGTCAGCAT AAAGAGGAAT ACATTCCAGC AGCATGTGGT 
GTATCGCCTT GGTCTGACTT CTCGCCCTGT ATGGGTCCCT GTGGCGGCTC TGGGCAGCGG 
CAACGTATTC GTAAAGTATG GAATAATCAC CAAGTTTATG GCATTGAGCA TGATGGCAAA 
GGCAGCCACG ACCCTTGCCG ACATATCAAA GTTCAGGAAG AGGTCAACTG TACTAATCCC 
AGCTGCGATA CCATCGTGCC CCTTCATTGC TACGAACAAC TCACGGAAAG CCATTGCCGC 
GACAGCGACG TAACCAATTA TTGGTATTAC GATCACATCA GCGATCAATG CGGCATCTAT 
TGGTCTGATC GTTGTGACAC CAATCGCAAC AAGTTCAAGT CGAAAGAGGA ATGCGAAGAC 
ACCTGCCGTT TGCCCCGACA CAAACAGGAG CTCCAATACG AGGGAGTCCA TCATGCCGTC 
GATTGTATGG TATCCGATTG GGTAGCCCAC AGCTGTAATG CCACCTGCGG CGAGGGAGTC 
CAGTTGAAGA CACGTCGTGT CCTGCGCACT CCCAAGTACG GGGGAAAATC CTGTCCAAAG 
CATTTGGTAA GGCACGATCG CTGCTATCAG CGTTGCGACG ACATGTATAC GGTAACTGGT 
GGCGGGGGCT ATGGTGAGCG GCGTCACATG ACCTCCAAGA TGCAGCCCAT GGAGCCGCGA 
GACGAATGCC GCTACTCAGA ATGGTCGGCA TGGACGCCAT GTACGGCCAG TTGTGGGGAT 
AATGCAGTAA GACAACGTAC AAGGACACTT CTCAATACGG ATCTTAGCTA CAAGTGCAAG 
GATCGTGTAC GAATTGAAAA GTGCGTTATG ATGCCCTGTC TAATGAGTAG CAACGATGAG 
GCAGACAAGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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