Dpse\GA28818 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA28818
Entrez Gene ID	6902623
Full Name	GA28818 gene product from transcript GA28818-RD
Synonyms	GA28818,dpse_GLEANR_9023
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015181239.1	XP_015036725.1	uncharacterized protein, isoform B
XM_002133113.2	XP_002133149.2	uncharacterized protein, isoform D
XM_015181240.1	XP_015036726.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA28818 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA28818 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe18637	XM_015181239.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28818), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODe18378	XM_002133113.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA28818), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODe18638	XM_015181240.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28818), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA28818 ( XM_015181239.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036725.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28818), mRNA.

Price & Availability↑

CloneID	ODe18637
Clone ID Related Accession (Same CDS sequence)	XM_015181239.1
Accession Version	XM_015181239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGG CCAGCACCAG CAGTCGACCG CCTCCGTCGC CGATTGCGGC GCCCATTTAT 
GATGGCTATG CCACGATCCG TGCGAGGGGC CTGACCCGTT CCTGTTCCAT ACGCGCCGCA 
GAGGCCACCG AAGCGGCTGC CTCCTCACAG CCACCGGCCC ATCAGCCGCA CATACTGCAG 
CCCCAGAGGA AGAGAACAGT GTCGCTGAGG AGCACCTACA GCCAGGGGAA TGGCGAGGAG 
ATCTATGCCC GGGCGAAGCC CGTGAGGACA CCAACCATTG GAGGAGATCT GGAGCCGATT 
TATGCGAGCA CCACATCGAA TCCCGTGGGC TTTCGACCGC TCTCTGACGC CTCCAGCTCC 
TCGGGCGGCG GGAGCATGCC CTGCGACTAC CAGAACGCAA AGAAGAACTC CCGCTTCTCC 
CTGGACTCCC TGCAGAGCAG TGCCACGCCC AAGACCCCGG ACTACAATAC AGCGGGGGAG 
GAGGAGCACA TCTTCCTGTA CACCGCACCG GACAGCCCCA AGCGTCGGTT GGACAGCGGT 
CTGCGGAGCT CCTACGACAG CTCCGAGAGG GGTTGCTACT CGCCTCTACC GAAGATCAGT 
TTCGATATGC AGGAGCGAGA GCGGGAGCGG GAGCGCGATC CGAGGATACG TCCGCCCACG 
TTGCGCCACA ATTCCGTGGG ATCCACGGGC TGTCGCCGAC CCATGACGCT GTCTCTCACG 
GAGGCGCCCC GTTCGCCCAC AGGCCTACCG CCGCCCGCAC CCGTGCGCCG CGAGAGCCTC 
TATGCCAAGC CAAAGATCTA TGCCACGCCC ACGAGGCCGC CGACCAGCAC ACGCCTAAAT 
CTGTACGAGC TGCCGATAGT CACGCAGCGG AGCAACAGTG CGGAGCTGTT GGAGAGGTCC 
TTCACGAGCC AGAGCCTCGC CACCATCGAC CAGGATGAGC TCCTGGTGGT CCCCAACGCC 
ATGCCCATGC TCCCGAATGC CGTGGATCGG CGCTACCACA CGCTGGGGCA CATGCGGGTC 
AAGAGCATGG CGGCGGGTCT GCACCGCAGC CACGATGTGC CCGTGGGCTC CATGCTGGAC 
ATGGCCACCG GCGGGCACTG GCGGACGCAT CGAGGCGGCA ACAAGTCGGT GGGGAATATC 
TACGCCTCGG CGGATGCGGC CGTCAGCGAT CTGAATCGCA TGGCTCCGGA GTACATGGAT 
CCGCTGGACT TCAAGATCGG CTGCCAGACG ACGCTGAGGA GCAAGCCGCT GATTCCCTGG 
TACGAGCTGG CCATCCGGAG GGACTTCAAG CGGCAGTCGT GTCCGCCGAT CAGTGGGGGG 
GCAGGGGGAG GCCAACCATC GCTGACATTG GCCGCCTCGC CGCAAGAGGA GCTCGAACAG 
GCCCATGTGG TGACGGCGTT TGAACAGTCG CTGACCAACA TGACCAATCG CCTGCAGCAG 
CTGACGGCGA CGTCGGAGCG CAAGGACGGA GAGCTGACGG ACATGCGGCA GACCATCGAG 
CTGCTGAGGA AGCAGTCCAT CCAGGCGGGC CTCACCACCG CGCATATGCA GAGCATGGGA 
CAGAATCAGA ATCACCAGCT GCAGTCGCAG CCCGGCGACC AGAAGCCACC GCCAGGACCG 
GGGGGCAGCA GGTCCGCCAG CAATGTCAAT GGCAACGGCA CTGGTAATGG CAATGGCAAC 
GGCATACCCA TCGGATTGGG AATGGTGCGG CAGCACAGCA CGGACTCGAT GTGCTCCTTG 
AATTCCATTA GCTCGGGATG CTCGGCGGCG CAGGACAAGA ACAAGGCCAA CAAGAAGAAG 
GGGTGGCTGC GCAGCAGCTT CACCAAGGCC TTCTCGCGGA ACGCCAAGAT CTCGAAGACC 
AGTCGCCATG TGGGACACCA CCACCACCAC AATCATCACG GACAGGAGCA CAGCTCCGGG 
AAGACACCGC TCCCGAATGG TCATGGCGAG CCGCTGGGTC TGGCGGCCCT GGCCACCCAA 
CCGGCGCCTC CTTTGCCTAA CAGCAAGCAG GGCAGTCCCG CCAAGACCCT GACGCTGATC 
GACAACGCCA AGCCCATCGA TGCCATCGAC CAGGAGGACC AGCAGGTGGT GGAGGACCTC 
AAGAAGCAGC TGCGCGAGAA GGATCTCGTC CTCACAGACA TTCGGCTAGA GGCCCTTAGC 
TCCGCCTCGC AGCTGGAGAG CCTCAAGGAC ATGATGAACA AGATGCGGTC CGAGATGATG 
TCCCTCAAGC AGAACAACGA GCGTCTGCAG AAGCTGGTGA CCACCCGCTC CCTGGCCGGC 
TCCGAGGTGA GCCTCGGACA GGCGATCAGC CCCAACGGAT CGGTGGCCGG CAGCTCTGAG 
GTCTCGCGCC GCTACTCGCT GGCCGACGGC AGTGCGCGAC CGCCCATGGA GCTGCCACCA 
CGCCTCAGCG AGGAGTTGGA GGAGGAGTGC ATGCCCCCAG CACCGGCGCC AGAGCCCCCA 
CCACCGCCAG CACCAAACAC CGCCGCTGTG GCGCCTCTGA GTCCCAGCAC ACACATTGAT 
CTGACGCCTC CACCACCGGC CCTGGAGACG GCGCCGCTGG CCAGCCCCGT GCACATGCCC 
AGCGCCGTCG AGGATCTGCC CGACGTGTGC GACGGCAAGA AGATTGCCAT CGCCTGCTAC 
CTGGGACAGC CGGAGTCGTT TGCCAAGTAC TGCGAGGAGC TGCAGGAGCT GGATGGCTTC 
TATGCCAATG GCTCGATCAT CGCCAGCCAC GAGGCCGACC CCCAGGCGCA GGGCGAGCGC 
AAGGTGAGCT TTGCAGCCGC CAGCTCAAAC GAGTTCATCG TCGCCTGCAC CTACATCTCG 
GGCAAGACGA CCTGGCAGAA TCTCGACTAC ATTGTGCGCA AGACCTTTAA GGACTATGTG 
GCTCGCATCG ATCCAGGCAC CAATCTGGGC CTGAACACGG ACTCCATCAC GTCGTACCAT 
CTGGGAGAGG CCAAGCGCGG GCCGGAGATG GGCTTCCCCG AGCTCCTGCC CTGCGGCTAC 
ATTGTGGGCA GTGTCCGGAC GCTGTATATC TGCCTGCAGG GCGTTGGGAG TCTGGCCTTC 
GACAGCCTCA TTCCACGCAG CATTGTGCAC CGCTACATCA GTCTCCTCAC GGAGCACAGG 
CGGCTCATCC TGTGCGGTCC CAGCGGCACT GGGAAGTCCT ATCTGGCGCG TCGCCTAGCC 
GAGTTCCTAG TGGCGCGTGC GGGACGGGGA AATCCCTCAG AGGCCATTGC CACGTTCAAC 
GTGGACCACA AATCCTCGAA GGATCTGCGG CAGTATCTGG GCCACATTGC CGAGCAGGCG 
GCCATTGCAA ATGGCGCTTC GGAGCTGCCT TCTGTGATCA TTCTGGACAA CTTGCACCAT 
GCCTCTGCCC TGGGCGATGT CTTCTCCTGC CTGCTGAGCG CCGGGCCGGC CAACAAATTG 
CCCTGCATTA TTGGCACCAT GTCGCAGGCC ACCTGCAACA CGACCAATCT TCAGCTGCAC 
CACAATTTCC GATGGGTCCT TACGGCCAAT CATATGGAGC CCGTCAAGGG CTTCCTTGGA 
CGCTTCCTGC GGCGGCGACT GTTCCAGCTG GAGCTGCAGA CGCAGCATGC CCAGCCAGAG 
CTGGCGTCGG TCTTGGCCTG GCTGCCCTCC GTCTGGCAGC ACATCAATCG TTTCCTGGAA 
GTCCACAGCT CCAGTGACGT GACTATTGGC CCGCGTCTGT TCCTCTCCTG CCCGCTGGAC 
TTGAAGGACT CGCAGGTGTG GTTCACCGAC ATTTGGAACT ATCATCTGTC CCCCTATCTG 
GTCGAGGCTG TGCGCGAGGG AGTGCAGCTC TATGGCCGCC GGGGCGGAGC CTGGAACGAT 
CCTTCTGTCT TCATACGCAA CTCCTATCCC TGGCCCTATG GGCCCGACTC AGTGCCACCG 
CTGCGACAGA TCAATGCAGA GGATGTGGGC CTAGAAGGCG TCGCATTGAC AAACGGGGAG 
AACCAGGATC CTCTGCTCAA TATGCTGATG CGCCTGCAGG AGGCTGCCAA CTACTCGGAG 
GCGCAGGATC AGGAGTCGGA CTGCGCCAGT CTGGACTCCA ACGTGACGCC GGACAGCTCT 
GCGGGCGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036725.1
CDS	323..4417
Translation	MSAASTSSRPPPSPIAAPIYDGYATIRARGLTRSCSIRAAEATEAAASSQPPAHQPHILQPQRKRTVSLRSTYSQGNGEEIYARAKPVRTPTIGGDLEPIYASTTSNPVGFRPLSDASSSSGGGSMPCDYQNAKKNSRFSLDSLQSSATPKTPDYNTAGEEEHIFLYTAPDSPKRRLDSGLRSSYDSSERGCYSPLPKISFDMQERERERERDPRIRPPTLRHNSVGSTGCRRPMTLSLTEAPRSPTGLPPPAPVRRESLYAKPKIYATPTRPPTSTRLNLYELPIVTQRSNSAELLERSFTSQSLATIDQDELLVVPNAMPMLPNAVDRRYHTLGHMRVKSMAAGLHRSHDVPVGSMLDMATGGHWRTHRGGNKSVGNIYASADAAVSDLNRMAPEYMDPLDFKIGCQTTLRSKPLIPWYELAIRRDFKRQSCPPISGGAGGGQPSLTLAASPQEELEQAHVVTAFEQSLTNMTNRLQQLTATSERKDGELTDMRQTIELLRKQSIQAGLTTAHMQSMGQNQNHQLQSQPGDQKPPPGPGGSRSASNVNGNGTGNGNGNGIPIGLGMVRQHSTDSMCSLNSISSGCSAAQDKNKANKKKGWLRSSFTKAFSRNAKISKTSRHVGHHHHHNHHGQEHSSGKTPLPNGHGEPLGLAALATQPAPPLPNSKQGSPAKTLTLIDNAKPIDAIDQEDQQVVEDLKKQLREKDLVLTDIRLEALSSASQLESLKDMMNKMRSEMMSLKQNNERLQKLVTTRSLAGSEVSLGQAISPNGSVAGSSEVSRRYSLADGSARPPMELPPRLSEELEEECMPPAPAPEPPPPPAPNTAAVAPLSPSTHIDLTPPPPALETAPLASPVHMPSAVEDLPDVCDGKKIAIACYLGQPESFAKYCEELQELDGFYANGSIIASHEADPQAQGERKVSFAAASSNEFIVACTYISGKTTWQNLDYIVRKTFKDYVARIDPGTNLGLNTDSITSYHLGEAKRGPEMGFPELLPCGYIVGSVRTLYICLQGVGSLAFDSLIPRSIVHRYISLLTEHRRLILCGPSGTGKSYLARRLAEFLVARAGRGNPSEAIATFNVDHKSSKDLRQYLGHIAEQAAIANGASELPSVIILDNLHHASALGDVFSCLLSAGPANKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLGRFLRRRLFQLELQTQHAQPELASVLAWLPSVWQHINRFLEVHSSSDVTIGPRLFLSCPLDLKDSQVWFTDIWNYHLSPYLVEAVREGVQLYGRRGGAWNDPSVFIRNSYPWPYGPDSVPPLRQINAEDVGLEGVALTNGENQDPLLNMLMRLQEAANYSEAQDQESDCASLDSNVTPDSSAGAE

Target ORF information:

RefSeq Version	XM_015181239.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28818), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181239.1

ORF Insert Sequence:

ATGTCTGCGG CCAGCACCAG CAGTCGACCG CCTCCGTCGC CGATTGCGGC GCCCATTTAT 
GATGGCTATG CCACGATCCG TGCGAGGGGC CTGACCCGTT CCTGTTCCAT ACGCGCCGCA 
GAGGCCACCG AAGCGGCTGC CTCCTCACAG CCACCGGCCC ATCAGCCGCA CATACTGCAG 
CCCCAGAGGA AGAGAACAGT GTCGCTGAGG AGCACCTACA GCCAGGGGAA TGGCGAGGAG 
ATCTATGCCC GGGCGAAGCC CGTGAGGACA CCAACCATTG GAGGAGATCT GGAGCCGATT 
TATGCGAGCA CCACATCGAA TCCCGTGGGC TTTCGACCGC TCTCTGACGC CTCCAGCTCC 
TCGGGCGGCG GGAGCATGCC CTGCGACTAC CAGAACGCAA AGAAGAACTC CCGCTTCTCC 
CTGGACTCCC TGCAGAGCAG TGCCACGCCC AAGACCCCGG ACTACAATAC AGCGGGGGAG 
GAGGAGCACA TCTTCCTGTA CACCGCACCG GACAGCCCCA AGCGTCGGTT GGACAGCGGT 
CTGCGGAGCT CCTACGACAG CTCCGAGAGG GGTTGCTACT CGCCTCTACC GAAGATCAGT 
TTCGATATGC AGGAGCGAGA GCGGGAGCGG GAGCGCGATC CGAGGATACG TCCGCCCACG 
TTGCGCCACA ATTCCGTGGG ATCCACGGGC TGTCGCCGAC CCATGACGCT GTCTCTCACG 
GAGGCGCCCC GTTCGCCCAC AGGCCTACCG CCGCCCGCAC CCGTGCGCCG CGAGAGCCTC 
TATGCCAAGC CAAAGATCTA TGCCACGCCC ACGAGGCCGC CGACCAGCAC ACGCCTAAAT 
CTGTACGAGC TGCCGATAGT CACGCAGCGG AGCAACAGTG CGGAGCTGTT GGAGAGGTCC 
TTCACGAGCC AGAGCCTCGC CACCATCGAC CAGGATGAGC TCCTGGTGGT CCCCAACGCC 
ATGCCCATGC TCCCGAATGC CGTGGATCGG CGCTACCACA CGCTGGGGCA CATGCGGGTC 
AAGAGCATGG CGGCGGGTCT GCACCGCAGC CACGATGTGC CCGTGGGCTC CATGCTGGAC 
ATGGCCACCG GCGGGCACTG GCGGACGCAT CGAGGCGGCA ACAAGTCGGT GGGGAATATC 
TACGCCTCGG CGGATGCGGC CGTCAGCGAT CTGAATCGCA TGGCTCCGGA GTACATGGAT 
CCGCTGGACT TCAAGATCGG CTGCCAGACG ACGCTGAGGA GCAAGCCGCT GATTCCCTGG 
TACGAGCTGG CCATCCGGAG GGACTTCAAG CGGCAGTCGT GTCCGCCGAT CAGTGGGGGG 
GCAGGGGGAG GCCAACCATC GCTGACATTG GCCGCCTCGC CGCAAGAGGA GCTCGAACAG 
GCCCATGTGG TGACGGCGTT TGAACAGTCG CTGACCAACA TGACCAATCG CCTGCAGCAG 
CTGACGGCGA CGTCGGAGCG CAAGGACGGA GAGCTGACGG ACATGCGGCA GACCATCGAG 
CTGCTGAGGA AGCAGTCCAT CCAGGCGGGC CTCACCACCG CGCATATGCA GAGCATGGGA 
CAGAATCAGA ATCACCAGCT GCAGTCGCAG CCCGGCGACC AGAAGCCACC GCCAGGACCG 
GGGGGCAGCA GGTCCGCCAG CAATGTCAAT GGCAACGGCA CTGGTAATGG CAATGGCAAC 
GGCATACCCA TCGGATTGGG AATGGTGCGG CAGCACAGCA CGGACTCGAT GTGCTCCTTG 
AATTCCATTA GCTCGGGATG CTCGGCGGCG CAGGACAAGA ACAAGGCCAA CAAGAAGAAG 
GGGTGGCTGC GCAGCAGCTT CACCAAGGCC TTCTCGCGGA ACGCCAAGAT CTCGAAGACC 
AGTCGCCATG TGGGACACCA CCACCACCAC AATCATCACG GACAGGAGCA CAGCTCCGGG 
AAGACACCGC TCCCGAATGG TCATGGCGAG CCGCTGGGTC TGGCGGCCCT GGCCACCCAA 
CCGGCGCCTC CTTTGCCTAA CAGCAAGCAG GGCAGTCCCG CCAAGACCCT GACGCTGATC 
GACAACGCCA AGCCCATCGA TGCCATCGAC CAGGAGGACC AGCAGGTGGT GGAGGACCTC 
AAGAAGCAGC TGCGCGAGAA GGATCTCGTC CTCACAGACA TTCGGCTAGA GGCCCTTAGC 
TCCGCCTCGC AGCTGGAGAG CCTCAAGGAC ATGATGAACA AGATGCGGTC CGAGATGATG 
TCCCTCAAGC AGAACAACGA GCGTCTGCAG AAGCTGGTGA CCACCCGCTC CCTGGCCGGC 
TCCGAGGTGA GCCTCGGACA GGCGATCAGC CCCAACGGAT CGGTGGCCGG CAGCTCTGAG 
GTCTCGCGCC GCTACTCGCT GGCCGACGGC AGTGCGCGAC CGCCCATGGA GCTGCCACCA 
CGCCTCAGCG AGGAGTTGGA GGAGGAGTGC ATGCCCCCAG CACCGGCGCC AGAGCCCCCA 
CCACCGCCAG CACCAAACAC CGCCGCTGTG GCGCCTCTGA GTCCCAGCAC ACACATTGAT 
CTGACGCCTC CACCACCGGC CCTGGAGACG GCGCCGCTGG CCAGCCCCGT GCACATGCCC 
AGCGCCGTCG AGGATCTGCC CGACGTGTGC GACGGCAAGA AGATTGCCAT CGCCTGCTAC 
CTGGGACAGC CGGAGTCGTT TGCCAAGTAC TGCGAGGAGC TGCAGGAGCT GGATGGCTTC 
TATGCCAATG GCTCGATCAT CGCCAGCCAC GAGGCCGACC CCCAGGCGCA GGGCGAGCGC 
AAGGTGAGCT TTGCAGCCGC CAGCTCAAAC GAGTTCATCG TCGCCTGCAC CTACATCTCG 
GGCAAGACGA CCTGGCAGAA TCTCGACTAC ATTGTGCGCA AGACCTTTAA GGACTATGTG 
GCTCGCATCG ATCCAGGCAC CAATCTGGGC CTGAACACGG ACTCCATCAC GTCGTACCAT 
CTGGGAGAGG CCAAGCGCGG GCCGGAGATG GGCTTCCCCG AGCTCCTGCC CTGCGGCTAC 
ATTGTGGGCA GTGTCCGGAC GCTGTATATC TGCCTGCAGG GCGTTGGGAG TCTGGCCTTC 
GACAGCCTCA TTCCACGCAG CATTGTGCAC CGCTACATCA GTCTCCTCAC GGAGCACAGG 
CGGCTCATCC TGTGCGGTCC CAGCGGCACT GGGAAGTCCT ATCTGGCGCG TCGCCTAGCC 
GAGTTCCTAG TGGCGCGTGC GGGACGGGGA AATCCCTCAG AGGCCATTGC CACGTTCAAC 
GTGGACCACA AATCCTCGAA GGATCTGCGG CAGTATCTGG GCCACATTGC CGAGCAGGCG 
GCCATTGCAA ATGGCGCTTC GGAGCTGCCT TCTGTGATCA TTCTGGACAA CTTGCACCAT 
GCCTCTGCCC TGGGCGATGT CTTCTCCTGC CTGCTGAGCG CCGGGCCGGC CAACAAATTG 
CCCTGCATTA TTGGCACCAT GTCGCAGGCC ACCTGCAACA CGACCAATCT TCAGCTGCAC 
CACAATTTCC GATGGGTCCT TACGGCCAAT CATATGGAGC CCGTCAAGGG CTTCCTTGGA 
CGCTTCCTGC GGCGGCGACT GTTCCAGCTG GAGCTGCAGA CGCAGCATGC CCAGCCAGAG 
CTGGCGTCGG TCTTGGCCTG GCTGCCCTCC GTCTGGCAGC ACATCAATCG TTTCCTGGAA 
GTCCACAGCT CCAGTGACGT GACTATTGGC CCGCGTCTGT TCCTCTCCTG CCCGCTGGAC 
TTGAAGGACT CGCAGGTGTG GTTCACCGAC ATTTGGAACT ATCATCTGTC CCCCTATCTG 
GTCGAGGCTG TGCGCGAGGG AGTGCAGCTC TATGGCCGCC GGGGCGGAGC CTGGAACGAT 
CCTTCTGTCT TCATACGCAA CTCCTATCCC TGGCCCTATG GGCCCGACTC AGTGCCACCG 
CTGCGACAGA TCAATGCAGA GGATGTGGGC CTAGAAGGCG TCGCATTGAC AAACGGGGAG 
AACCAGGATC CTCTGCTCAA TATGCTGATG CGCCTGCAGG AGGCTGCCAA CTACTCGGAG 
GCGCAGGATC AGGAGTCGGA CTGCGCCAGT CTGGACTCCA ACGTGACGCC GGACAGCTCT 
GCGGGCGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA28818 ( XM_002133113.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002133149.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA28818), mRNA.

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CloneID	ODe18378
Clone ID Related Accession (Same CDS sequence)	XM_002133113.2
Accession Version	XM_002133113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). On Dec 22, 2015 this sequence version replaced XM_002133113.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATA CATCGTCCTC ATCATCGGGA TCGCATGGTG GGGGCAAGAG TTCACGGAAA 
TGTCGAAGTC TACCCAACTC TCCGGATGTC AGAAGACAAA TAGCCCCCGC CTATAAGGTC 
AATGGCGGCA CTGCCATACC ACTGCCGGCC ACCGTTATGG TGCAGCGTCG CTGTCCACCG 
GATAAAGTGC GTCCACTGCC CCCGACACCA AATCATACGC CATCCATACC GGGACTGGGC 
AAGAGCGGAA GTGATTTCAA TACGAGCCGT CCAAACAGTC CGCCCACCAG CAATCATACA 
ATCCAAAGCC TCAAGAGTGG CAATAACAAC AGCCTACGTC CACCGAGTAT CAAGAGCAAC 
CACCAAAGCG GAATACCCTC CCCCAGTAGC CCCCAGACCA CGACGACGGC GGCTGCGGCA 
GCTCCCCAGA AGCACTCGAT GCTGGACAAG CTGAAGCTCT TCAACAAGGA GAAGCAGCAG 
AATGCAGTGA ATGCGGCGGC GGCGGCGGCG GCGGCGGCCA ACAAATCGCA GATCCAGCAG 
CAACAGTCGA AGCGCACCTC CTCCTCGTCG GGGTTTAGCT CGGCGCGTTC CGAGCGCTCC 
GACTCCAGTC TGAGCCTCAA CGATGGCCAC AACTCGTCGC AGCTGAAGCC CCCCAGCATC 
AGTGTGTCCT CGCAGAAGCC CCAACAGAAG CACAACACGA AGCAGTCGAA GCTGCTGGCG 
GCACAACAGA AGAAGGAGCA GACAAAGTCG AAGCTGGACA AAAAGGAAAA GAGTCCGGCC 
CGCTCGCTGC ACAAGGAAGA GTCCGGGAAT GAGTCGCGCA GCTCCACGAT GGGCAGGGCG 
GGCAAGAGCT CCCTGGCCAG AGGAGGCCTC GGTCTTGGCC TCACGGTGGA GAAGAACACA 
CTGAAGACAT CGTCCAAGTC CTCGCTGCAC TCCAAGTCCG AGTCGAAGAG CTCTCTAAAG 
GGTGTCGGAG CCCAGCAGCA GCAGCAGATG TTGCACAGTC CGAGTGGCAC GAGTCTGCCC 
AAACCCATTG CGGCGATCAA GGGCACCAGC AAGCTGCCGG CCCTAGCGGG AGGAGAGCTC 
CTGAAGCGGG AAACCAGCGA CATATCAGCG GCCAATCAGG CAACGCAGAC CACCCAGAAC 
ATATCCACCA TATCCGTGCA GGAATCACAG CAGCAGCCAC TGCCTCTGAG CACCACTACA 
CCACATATCC TTAAGGGTCC AGCGCCACCG CCGTACTACG CGAATAGCAG TCCACCCTCA 
CACATATCAC AAGGACATCC CGGCATGGGA GGATACCTCA GTGAGCCCAG CACTCCCCAG 
CACCAGCAGG GGATCTACGC GAGCAGCAGC GGCAGTCGTC TGCCACCGCC CAAGACGGCG 
CTGAGTGCCC CCCGGAAACT GGAGTACAAT GCAGGACCCC ATATCCTGTC ATCGCCTCCC 
CATCGAGGGG CAGGTCTGCA GCGTCCGATG CCCAACTCTG CCCCGAATAC ACCCACTGCA 
TCGCCGAACA AGTTCCACAC GGTACCCTCG AAGATTGTGG GCACCATTTA CGAGTCGAAG 
GAGGAGCAGC AGCAGCAGCC CCAGCAGACA GGCGGATGTT CCGCCGGAGC CTCAGTGCTG 
CCCATGAGAC CCCTCCTGCG GGGCTACAAC TCCCATGTGA CGCTGCCGAC ACGTGGAGCA 
CGCGGTGGCC ACCACCATCC GCATCCGCCT CAGTCGTATC TGGATTTCTG CGAGTCGGAT 
CTGGGGCAGG GCTACTGCAG CGATGGCGAC GCCCTGAGGA TTGGCAGCTC TCCGGCCACC 
AGTCGCTATC AAGACATCGA CAATGGCTAT CTGTCGGAGG GGAGCAGCGG CCTGAATGGA 
TCGATCGGCG GATCCACTGG TGGTGCGGTG TCCCACGGAA AACACTTTCT CAGGATGATG 
AGGGCCAGAA CACCGCTGCC AACAACCATT GAAGAGAGAA TCCGCAATAG TCGAGGCTCT 
CTAGACAGCA TTGGAACCGC CGCAAACGGC AGCTCTGCGG CCAGTCAGGC CAGCTCCAGC 
GGCGGCTCCA CCACCCACAG CAGCAGCAAC AGCAACAGCG CCGGCACCAC CGCCAACCAC 
AACAACAACC ACATCAACAA CAACAACGGA GGGAAAATGG ATGGCAGTCC ACACCATCGT 
CCGGGATCAC GCAATGGAAG GGATAACTGG AGCAAAATGC CAGAGCCGCT CAACGGCCAG 
AAGGGGGACA AATCGTCGGA GAAGTCATCG CCATCGCGCA GGAGCATGGG CGGTGGCAGC 
GGGGGATCCT CCAAGCAGGG TTCGCCCTCG TCTTCCTCCA GGACCAAGGG AGTCCCACCG 
AGTTTCGGGT ATGTGAAGAG GGCCAATGGC AGCATGTCCT CCACTGCAGA GCAGCAGAAT 
ATTGTCATGA TGATGGCAGC CGGAGGCGGA GGAGGACCGC AGCAGGTCAA TGGATTGCCC 
TGCGGCAGGA CCGCCCATGT GTCGGCGGTG CCAAGGACAG CAAATGGTCG AAAGATTGCA 
CCCGGCACTC AGACATTGCC CAATGATATG AACAAGCTAC CACCAAACAC ACAGTGTCGC 
AGCTTCTCAC TGACGGGACC CACAGCGGCA CAATTGAGTC AATCGATACG CGAGCGTTTG 
GCCACAGGAT CGCACAGTTT GCCCAAACCG GGCAGCGATA TGCATGTCTT TCAGCATAGA 
ATGTCCAATC GCCCTGGCAC CCGTCACGAT GGCTCTCTCT CCGACACCCA GACCTATGCC 
GAGGTCAAGC CCGAATACAG TTCGTATGCC ATGTGGCTGA AGCACAGCAA TACGGCCGGA 
TCACGTCTCT CGGATGGCGA TGCCATTGAG CAGCTGCAGA TTGGATCGCC AGCGATGACC 
CGTCACGGGC ACAAGATGAT CCACAATCGA TCGGGAGGAG GACCCCCTGG TGGACAAATG 
CCCATGCAGG GAAATGAGTC GCCCTACGTG CAGAGTCCTC GCATGAATCG GAGCAATAGC 
ATCAGATCCA CAAAATCGGA GAAAATGTAT CCCTCGATGC TGAGCCGCGC TGGGGACGTG 
GAAATCGAGC CGTACTATTG CCTGCCGGTG GGCACCAACG GAGTGCTGAG CGCCCAAATG 
GCAGCTGCTG TGGCGGCCCA GGCCCAAGCC CAAGCACAGG CCACGCAGGG AAATTCTGGC 
AACAATGTGG TGGGTGGTTC CGTGGCCTGG AGCCAGCCCA CATCGCCCAC GCCCCTCAAT 
CGAGGCCCCT TTGCGGCGTC AGTGGCAGCA GCGGCAGCAG CAGCAGCTGC TGCAGGGGTT 
CTATCGCCCA CTCACGGAAC GACCGCAGGA GCCACAGGGA TGGTTGGTGG CATGGGAATG 
GGACCCGGAC TCGGCTCCGG TCTAACTGGC GGACATCGCC TCACATATCC CAAGAAGAAC 
GACGAAGTGC ACGGCAGTGC CGCATCCCTG CTGTCGGGCG GCAGTTCCCT GTACGGATCC 
GCGGAGGAGC GGCAGGCCCA CGAGATACGG AGATTGAAGC GCGAACTGCA GGATGCACGC 
GACCAGGTGC TCAGCCTAAG CAGCCAGCTG TCAACCAATG CCCATGTGGT GACGGCGTTT 
GAACAGTCGC TGACCAACAT GACCAATCGC CTGCAGCAGC TGACGGCGAC GTCGGAGCGC 
AAGGACGGAG AGCTGACGGA CATGCGGCAG ACCATCGAGC TGCTGAGGAA GCAGTCCATC 
CAGGCGGGCC TCACCACCGC GCATATGCAG AGCATGGGAC AGAATCAGAA TCACCAGCTG 
CAGTCGCAGC CCGGCGACCA GAAGCCACCG CCAGGACCGG GGGGCAGCAG GTCCGCCAGC 
AATGTCAATG GCAACGGCAC TGGTAATGGC AATGGCAACG GCATACCCAT CGGATTGGGA 
ATGGTGCGGC AGCACAGCAC GGACTCGATG TGCTCCTTGA ATTCCATTAG CTCGGGATGC 
TCGGCGGCGC AGGACAAGAA CAAGGCCAAC AAGAAGAAGG GGTGGCTGCG CAGCAGCTTC 
ACCAAGGCCT TCTCGCGGAA CGCCAAGATC TCGAAGACCA GTCGCCATGT GGGACACCAC 
CACCACCACA ATCATCACGG ACAGGAGCAC AGCTCCGGGA AGACACCGCT CCCGAATGGT 
CATGGCGAGC CGCTGGGTCT GGCGGCCCTG GCCACCCAAC CGGCGCCTCC TTTGCCTAAC 
AGCAAGCAGG GCAGTCCCGC CAAGACCCTG ACGCTGATCG ACAACGCCAA GCCCATCGAT 
GCCATCGACC AGGAGGACCA GCAGGTGGTG GAGGACCTCA AGAAGCAGCT GCGCGAGAAG 
GATCTCGTCC TCACAGACAT TCGGCTAGAG GCCCTTAGCT CCGCCTCGCA GCTGGAGAGC 
CTCAAGGACA TGATGAACAA GATGCGGTCC GAGATGATGT CCCTCAAGCA GAACAACGAG 
CGTCTGCAGA AGCTGGTGAC CACCCGCTCC CTGGCCGGCT CCGAGGTGAG CCTCGGACAG 
GCGATCAGCC CCAACGGATC GGTGGCCGGC AGCTCTGAGG TCTCGCGCCG CTACTCGCTG 
GCCGACGGCA GTGCGCGACC GCCCATGGAG CTGCCACCAC GCCTCAGCGA GGAGTTGGAG 
GAGGAGTGCA TGCCCCCAGC ACCGGCGCCA GAGCCCCCAC CACCGCCAGC ACCAAACACC 
GCCGCTGTGG CGCCTCTGAG TCCCAGCACA CACATTGATC TGACGCCTCC ACCACCGGCC 
CTGGAGACGG CGCCGCTGGC CAGCCCCGTG CACATGCCCA GCGCCGTCGA GGATCTGCCC 
GACGTGTGCG ACGGCAAGAA GATTGCCATC GCCTGCTACC TGGGACAGCC GGAGTCGTTT 
GCCAAGTACT GCGAGGAGCT GCAGGAGCTG GATGGCTTCT ATGCCAATGG CTCGATCATC 
GCCAGCCACG AGGCCGACCC CCAGGCGCAG GGCGAGCGCA AGGTGAGCTT TGCAGCCGCC 
AGCTCAAACG AGTTCATCGT CGCCTGCACC TACATCTCGG GCAAGACGAC CTGGCAGAAT 
CTCGACTACA TTGTGCGCAA GACCTTTAAG GACTATGTGG CTCGCATCGA TCCAGGCACC 
AATCTGGGCC TGAACACGGA CTCCATCACG TCGTACCATC TGGGAGAGGC CAAGCGCGGG 
CCGGAGATGG GCTTCCCCGA GCTCCTGCCC TGCGGCTACA TTGTGGGCAG TGTCCGGACG 
CTGTATATCT GCCTGCAGGG CGTTGGGAGT CTGGCCTTCG ACAGCCTCAT TCCACGCAGC 
ATTGTGCACC GCTACATCAG TCTCCTCACG GAGCACAGGC GGCTCATCCT GTGCGGTCCC 
AGCGGCACTG GGAAGTCCTA TCTGGCGCGT CGCCTAGCCG AGTTCCTAGT GGCGCGTGCG 
GGACGGGGAA ATCCCTCAGA GGCCATTGCC ACGTTCAACG TGGACCACAA ATCCTCGAAG 
GATCTGCGGC AGTATCTGGG CCACATTGCC GAGCAGGCGG CCATTGCAAA TGGCGCTTCG 
GAGCTGCCTT CTGTGATCAT TCTGGACAAC TTGCACCATG CCTCTGCCCT GGGCGATGTC 
TTCTCCTGCC TGCTGAGCGC CGGGCCGGCC AACAAATTGC CCTGCATTAT TGGCACCATG 
TCGCAGGCCA CCTGCAACAC GACCAATCTT CAGCTGCACC ACAATTTCCG ATGGGTCCTT 
ACGGCCAATC ATATGGAGCC CGTCAAGGGC TTCCTTGGAC GCTTCCTGCG GCGGCGACTG 
TTCCAGCTGG AGCTGCAGAC GCAGCATGCC CAGCCAGAGC TGGCGTCGGT CTTGGCCTGG 
CTGCCCTCCG TCTGGCAGCA CATCAATCGT TTCCTGGAAG TCCACAGCTC CAGTGACGTG 
ACTATTGGCC CGCGTCTGTT CCTCTCCTGC CCGCTGGACT TGAAGGACTC GCAGGTGTGG 
TTCACCGACA TTTGGAACTA TCATCTGTCC CCCTATCTGG TCGAGGCTGT GCGCGAGGGA 
GTGCAGCTCT ATGGCCGCCG GGGCGGAGCC TGGAACGATC CTTCTGTCTT CATACGCAAC 
TCCTATCCCT GGCCCTATGG GCCCGACTCA GTGCCACCGC TGCGACAGAT CAATGCAGAG 
GATGTGGGCC TAGAAGGCGT CGCATTGACA AACGGGGAGA ACCAGGATCC TCTGCTCAAT 
ATGCTGATGC GCCTGCAGGA GGCTGCCAAC TACTCGGAGG CGCAGGATCA GGAGTCGGAC 
TGCGCCAGTC TGGACTCCAA CGTGACGCCG GACAGCTCTG CGGGCGCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002133149.2
CDS	121..6414
Translation	MGNTSSSSSGSHGGGKSSRKCRSLPNSPDVRRQIAPAYKVNGGTAIPLPATVMVQRRCPPDKVRPLPPTPNHTPSIPGLGKSGSDFNTSRPNSPPTSNHTIQSLKSGNNNSLRPPSIKSNHQSGIPSPSSPQTTTTAAAAAPQKHSMLDKLKLFNKEKQQNAVNAAAAAAAAANKSQIQQQQSKRTSSSSGFSSARSERSDSSLSLNDGHNSSQLKPPSISVSSQKPQQKHNTKQSKLLAAQQKKEQTKSKLDKKEKSPARSLHKEESGNESRSSTMGRAGKSSLARGGLGLGLTVEKNTLKTSSKSSLHSKSESKSSLKGVGAQQQQQMLHSPSGTSLPKPIAAIKGTSKLPALAGGELLKRETSDISAANQATQTTQNISTISVQESQQQPLPLSTTTPHILKGPAPPPYYANSSPPSHISQGHPGMGGYLSEPSTPQHQQGIYASSSGSRLPPPKTALSAPRKLEYNAGPHILSSPPHRGAGLQRPMPNSAPNTPTASPNKFHTVPSKIVGTIYESKEEQQQQPQQTGGCSAGASVLPMRPLLRGYNSHVTLPTRGARGGHHHPHPPQSYLDFCESDLGQGYCSDGDALRIGSSPATSRYQDIDNGYLSEGSSGLNGSIGGSTGGAVSHGKHFLRMMRARTPLPTTIEERIRNSRGSLDSIGTAANGSSAASQASSSGGSTTHSSSNSNSAGTTANHNNNHINNNNGGKMDGSPHHRPGSRNGRDNWSKMPEPLNGQKGDKSSEKSSPSRRSMGGGSGGSSKQGSPSSSSRTKGVPPSFGYVKRANGSMSSTAEQQNIVMMMAAGGGGGPQQVNGLPCGRTAHVSAVPRTANGRKIAPGTQTLPNDMNKLPPNTQCRSFSLTGPTAAQLSQSIRERLATGSHSLPKPGSDMHVFQHRMSNRPGTRHDGSLSDTQTYAEVKPEYSSYAMWLKHSNTAGSRLSDGDAIEQLQIGSPAMTRHGHKMIHNRSGGGPPGGQMPMQGNESPYVQSPRMNRSNSIRSTKSEKMYPSMLSRAGDVEIEPYYCLPVGTNGVLSAQMAAAVAAQAQAQAQATQGNSGNNVVGGSVAWSQPTSPTPLNRGPFAASVAAAAAAAAAAGVLSPTHGTTAGATGMVGGMGMGPGLGSGLTGGHRLTYPKKNDEVHGSAASLLSGGSSLYGSAEERQAHEIRRLKRELQDARDQVLSLSSQLSTNAHVVTAFEQSLTNMTNRLQQLTATSERKDGELTDMRQTIELLRKQSIQAGLTTAHMQSMGQNQNHQLQSQPGDQKPPPGPGGSRSASNVNGNGTGNGNGNGIPIGLGMVRQHSTDSMCSLNSISSGCSAAQDKNKANKKKGWLRSSFTKAFSRNAKISKTSRHVGHHHHHNHHGQEHSSGKTPLPNGHGEPLGLAALATQPAPPLPNSKQGSPAKTLTLIDNAKPIDAIDQEDQQVVEDLKKQLREKDLVLTDIRLEALSSASQLESLKDMMNKMRSEMMSLKQNNERLQKLVTTRSLAGSEVSLGQAISPNGSVAGSSEVSRRYSLADGSARPPMELPPRLSEELEEECMPPAPAPEPPPPPAPNTAAVAPLSPSTHIDLTPPPPALETAPLASPVHMPSAVEDLPDVCDGKKIAIACYLGQPESFAKYCEELQELDGFYANGSIIASHEADPQAQGERKVSFAAASSNEFIVACTYISGKTTWQNLDYIVRKTFKDYVARIDPGTNLGLNTDSITSYHLGEAKRGPEMGFPELLPCGYIVGSVRTLYICLQGVGSLAFDSLIPRSIVHRYISLLTEHRRLILCGPSGTGKSYLARRLAEFLVARAGRGNPSEAIATFNVDHKSSKDLRQYLGHIAEQAAIANGASELPSVIILDNLHHASALGDVFSCLLSAGPANKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLGRFLRRRLFQLELQTQHAQPELASVLAWLPSVWQHINRFLEVHSSSDVTIGPRLFLSCPLDLKDSQVWFTDIWNYHLSPYLVEAVREGVQLYGRRGGAWNDPSVFIRNSYPWPYGPDSVPPLRQINAEDVGLEGVALTNGENQDPLLNMLMRLQEAANYSEAQDQESDCASLDSNVTPDSSAGAE

Target ORF information:

RefSeq Version	XM_002133113.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA28818), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002133113.2

ORF Insert Sequence:

ATGGGCAATA CATCGTCCTC ATCATCGGGA TCGCATGGTG GGGGCAAGAG TTCACGGAAA 
TGTCGAAGTC TACCCAACTC TCCGGATGTC AGAAGACAAA TAGCCCCCGC CTATAAGGTC 
AATGGCGGCA CTGCCATACC ACTGCCGGCC ACCGTTATGG TGCAGCGTCG CTGTCCACCG 
GATAAAGTGC GTCCACTGCC CCCGACACCA AATCATACGC CATCCATACC GGGACTGGGC 
AAGAGCGGAA GTGATTTCAA TACGAGCCGT CCAAACAGTC CGCCCACCAG CAATCATACA 
ATCCAAAGCC TCAAGAGTGG CAATAACAAC AGCCTACGTC CACCGAGTAT CAAGAGCAAC 
CACCAAAGCG GAATACCCTC CCCCAGTAGC CCCCAGACCA CGACGACGGC GGCTGCGGCA 
GCTCCCCAGA AGCACTCGAT GCTGGACAAG CTGAAGCTCT TCAACAAGGA GAAGCAGCAG 
AATGCAGTGA ATGCGGCGGC GGCGGCGGCG GCGGCGGCCA ACAAATCGCA GATCCAGCAG 
CAACAGTCGA AGCGCACCTC CTCCTCGTCG GGGTTTAGCT CGGCGCGTTC CGAGCGCTCC 
GACTCCAGTC TGAGCCTCAA CGATGGCCAC AACTCGTCGC AGCTGAAGCC CCCCAGCATC 
AGTGTGTCCT CGCAGAAGCC CCAACAGAAG CACAACACGA AGCAGTCGAA GCTGCTGGCG 
GCACAACAGA AGAAGGAGCA GACAAAGTCG AAGCTGGACA AAAAGGAAAA GAGTCCGGCC 
CGCTCGCTGC ACAAGGAAGA GTCCGGGAAT GAGTCGCGCA GCTCCACGAT GGGCAGGGCG 
GGCAAGAGCT CCCTGGCCAG AGGAGGCCTC GGTCTTGGCC TCACGGTGGA GAAGAACACA 
CTGAAGACAT CGTCCAAGTC CTCGCTGCAC TCCAAGTCCG AGTCGAAGAG CTCTCTAAAG 
GGTGTCGGAG CCCAGCAGCA GCAGCAGATG TTGCACAGTC CGAGTGGCAC GAGTCTGCCC 
AAACCCATTG CGGCGATCAA GGGCACCAGC AAGCTGCCGG CCCTAGCGGG AGGAGAGCTC 
CTGAAGCGGG AAACCAGCGA CATATCAGCG GCCAATCAGG CAACGCAGAC CACCCAGAAC 
ATATCCACCA TATCCGTGCA GGAATCACAG CAGCAGCCAC TGCCTCTGAG CACCACTACA 
CCACATATCC TTAAGGGTCC AGCGCCACCG CCGTACTACG CGAATAGCAG TCCACCCTCA 
CACATATCAC AAGGACATCC CGGCATGGGA GGATACCTCA GTGAGCCCAG CACTCCCCAG 
CACCAGCAGG GGATCTACGC GAGCAGCAGC GGCAGTCGTC TGCCACCGCC CAAGACGGCG 
CTGAGTGCCC CCCGGAAACT GGAGTACAAT GCAGGACCCC ATATCCTGTC ATCGCCTCCC 
CATCGAGGGG CAGGTCTGCA GCGTCCGATG CCCAACTCTG CCCCGAATAC ACCCACTGCA 
TCGCCGAACA AGTTCCACAC GGTACCCTCG AAGATTGTGG GCACCATTTA CGAGTCGAAG 
GAGGAGCAGC AGCAGCAGCC CCAGCAGACA GGCGGATGTT CCGCCGGAGC CTCAGTGCTG 
CCCATGAGAC CCCTCCTGCG GGGCTACAAC TCCCATGTGA CGCTGCCGAC ACGTGGAGCA 
CGCGGTGGCC ACCACCATCC GCATCCGCCT CAGTCGTATC TGGATTTCTG CGAGTCGGAT 
CTGGGGCAGG GCTACTGCAG CGATGGCGAC GCCCTGAGGA TTGGCAGCTC TCCGGCCACC 
AGTCGCTATC AAGACATCGA CAATGGCTAT CTGTCGGAGG GGAGCAGCGG CCTGAATGGA 
TCGATCGGCG GATCCACTGG TGGTGCGGTG TCCCACGGAA AACACTTTCT CAGGATGATG 
AGGGCCAGAA CACCGCTGCC AACAACCATT GAAGAGAGAA TCCGCAATAG TCGAGGCTCT 
CTAGACAGCA TTGGAACCGC CGCAAACGGC AGCTCTGCGG CCAGTCAGGC CAGCTCCAGC 
GGCGGCTCCA CCACCCACAG CAGCAGCAAC AGCAACAGCG CCGGCACCAC CGCCAACCAC 
AACAACAACC ACATCAACAA CAACAACGGA GGGAAAATGG ATGGCAGTCC ACACCATCGT 
CCGGGATCAC GCAATGGAAG GGATAACTGG AGCAAAATGC CAGAGCCGCT CAACGGCCAG 
AAGGGGGACA AATCGTCGGA GAAGTCATCG CCATCGCGCA GGAGCATGGG CGGTGGCAGC 
GGGGGATCCT CCAAGCAGGG TTCGCCCTCG TCTTCCTCCA GGACCAAGGG AGTCCCACCG 
AGTTTCGGGT ATGTGAAGAG GGCCAATGGC AGCATGTCCT CCACTGCAGA GCAGCAGAAT 
ATTGTCATGA TGATGGCAGC CGGAGGCGGA GGAGGACCGC AGCAGGTCAA TGGATTGCCC 
TGCGGCAGGA CCGCCCATGT GTCGGCGGTG CCAAGGACAG CAAATGGTCG AAAGATTGCA 
CCCGGCACTC AGACATTGCC CAATGATATG AACAAGCTAC CACCAAACAC ACAGTGTCGC 
AGCTTCTCAC TGACGGGACC CACAGCGGCA CAATTGAGTC AATCGATACG CGAGCGTTTG 
GCCACAGGAT CGCACAGTTT GCCCAAACCG GGCAGCGATA TGCATGTCTT TCAGCATAGA 
ATGTCCAATC GCCCTGGCAC CCGTCACGAT GGCTCTCTCT CCGACACCCA GACCTATGCC 
GAGGTCAAGC CCGAATACAG TTCGTATGCC ATGTGGCTGA AGCACAGCAA TACGGCCGGA 
TCACGTCTCT CGGATGGCGA TGCCATTGAG CAGCTGCAGA TTGGATCGCC AGCGATGACC 
CGTCACGGGC ACAAGATGAT CCACAATCGA TCGGGAGGAG GACCCCCTGG TGGACAAATG 
CCCATGCAGG GAAATGAGTC GCCCTACGTG CAGAGTCCTC GCATGAATCG GAGCAATAGC 
ATCAGATCCA CAAAATCGGA GAAAATGTAT CCCTCGATGC TGAGCCGCGC TGGGGACGTG 
GAAATCGAGC CGTACTATTG CCTGCCGGTG GGCACCAACG GAGTGCTGAG CGCCCAAATG 
GCAGCTGCTG TGGCGGCCCA GGCCCAAGCC CAAGCACAGG CCACGCAGGG AAATTCTGGC 
AACAATGTGG TGGGTGGTTC CGTGGCCTGG AGCCAGCCCA CATCGCCCAC GCCCCTCAAT 
CGAGGCCCCT TTGCGGCGTC AGTGGCAGCA GCGGCAGCAG CAGCAGCTGC TGCAGGGGTT 
CTATCGCCCA CTCACGGAAC GACCGCAGGA GCCACAGGGA TGGTTGGTGG CATGGGAATG 
GGACCCGGAC TCGGCTCCGG TCTAACTGGC GGACATCGCC TCACATATCC CAAGAAGAAC 
GACGAAGTGC ACGGCAGTGC CGCATCCCTG CTGTCGGGCG GCAGTTCCCT GTACGGATCC 
GCGGAGGAGC GGCAGGCCCA CGAGATACGG AGATTGAAGC GCGAACTGCA GGATGCACGC 
GACCAGGTGC TCAGCCTAAG CAGCCAGCTG TCAACCAATG CCCATGTGGT GACGGCGTTT 
GAACAGTCGC TGACCAACAT GACCAATCGC CTGCAGCAGC TGACGGCGAC GTCGGAGCGC 
AAGGACGGAG AGCTGACGGA CATGCGGCAG ACCATCGAGC TGCTGAGGAA GCAGTCCATC 
CAGGCGGGCC TCACCACCGC GCATATGCAG AGCATGGGAC AGAATCAGAA TCACCAGCTG 
CAGTCGCAGC CCGGCGACCA GAAGCCACCG CCAGGACCGG GGGGCAGCAG GTCCGCCAGC 
AATGTCAATG GCAACGGCAC TGGTAATGGC AATGGCAACG GCATACCCAT CGGATTGGGA 
ATGGTGCGGC AGCACAGCAC GGACTCGATG TGCTCCTTGA ATTCCATTAG CTCGGGATGC 
TCGGCGGCGC AGGACAAGAA CAAGGCCAAC AAGAAGAAGG GGTGGCTGCG CAGCAGCTTC 
ACCAAGGCCT TCTCGCGGAA CGCCAAGATC TCGAAGACCA GTCGCCATGT GGGACACCAC 
CACCACCACA ATCATCACGG ACAGGAGCAC AGCTCCGGGA AGACACCGCT CCCGAATGGT 
CATGGCGAGC CGCTGGGTCT GGCGGCCCTG GCCACCCAAC CGGCGCCTCC TTTGCCTAAC 
AGCAAGCAGG GCAGTCCCGC CAAGACCCTG ACGCTGATCG ACAACGCCAA GCCCATCGAT 
GCCATCGACC AGGAGGACCA GCAGGTGGTG GAGGACCTCA AGAAGCAGCT GCGCGAGAAG 
GATCTCGTCC TCACAGACAT TCGGCTAGAG GCCCTTAGCT CCGCCTCGCA GCTGGAGAGC 
CTCAAGGACA TGATGAACAA GATGCGGTCC GAGATGATGT CCCTCAAGCA GAACAACGAG 
CGTCTGCAGA AGCTGGTGAC CACCCGCTCC CTGGCCGGCT CCGAGGTGAG CCTCGGACAG 
GCGATCAGCC CCAACGGATC GGTGGCCGGC AGCTCTGAGG TCTCGCGCCG CTACTCGCTG 
GCCGACGGCA GTGCGCGACC GCCCATGGAG CTGCCACCAC GCCTCAGCGA GGAGTTGGAG 
GAGGAGTGCA TGCCCCCAGC ACCGGCGCCA GAGCCCCCAC CACCGCCAGC ACCAAACACC 
GCCGCTGTGG CGCCTCTGAG TCCCAGCACA CACATTGATC TGACGCCTCC ACCACCGGCC 
CTGGAGACGG CGCCGCTGGC CAGCCCCGTG CACATGCCCA GCGCCGTCGA GGATCTGCCC 
GACGTGTGCG ACGGCAAGAA GATTGCCATC GCCTGCTACC TGGGACAGCC GGAGTCGTTT 
GCCAAGTACT GCGAGGAGCT GCAGGAGCTG GATGGCTTCT ATGCCAATGG CTCGATCATC 
GCCAGCCACG AGGCCGACCC CCAGGCGCAG GGCGAGCGCA AGGTGAGCTT TGCAGCCGCC 
AGCTCAAACG AGTTCATCGT CGCCTGCACC TACATCTCGG GCAAGACGAC CTGGCAGAAT 
CTCGACTACA TTGTGCGCAA GACCTTTAAG GACTATGTGG CTCGCATCGA TCCAGGCACC 
AATCTGGGCC TGAACACGGA CTCCATCACG TCGTACCATC TGGGAGAGGC CAAGCGCGGG 
CCGGAGATGG GCTTCCCCGA GCTCCTGCCC TGCGGCTACA TTGTGGGCAG TGTCCGGACG 
CTGTATATCT GCCTGCAGGG CGTTGGGAGT CTGGCCTTCG ACAGCCTCAT TCCACGCAGC 
ATTGTGCACC GCTACATCAG TCTCCTCACG GAGCACAGGC GGCTCATCCT GTGCGGTCCC 
AGCGGCACTG GGAAGTCCTA TCTGGCGCGT CGCCTAGCCG AGTTCCTAGT GGCGCGTGCG 
GGACGGGGAA ATCCCTCAGA GGCCATTGCC ACGTTCAACG TGGACCACAA ATCCTCGAAG 
GATCTGCGGC AGTATCTGGG CCACATTGCC GAGCAGGCGG CCATTGCAAA TGGCGCTTCG 
GAGCTGCCTT CTGTGATCAT TCTGGACAAC TTGCACCATG CCTCTGCCCT GGGCGATGTC 
TTCTCCTGCC TGCTGAGCGC CGGGCCGGCC AACAAATTGC CCTGCATTAT TGGCACCATG 
TCGCAGGCCA CCTGCAACAC GACCAATCTT CAGCTGCACC ACAATTTCCG ATGGGTCCTT 
ACGGCCAATC ATATGGAGCC CGTCAAGGGC TTCCTTGGAC GCTTCCTGCG GCGGCGACTG 
TTCCAGCTGG AGCTGCAGAC GCAGCATGCC CAGCCAGAGC TGGCGTCGGT CTTGGCCTGG 
CTGCCCTCCG TCTGGCAGCA CATCAATCGT TTCCTGGAAG TCCACAGCTC CAGTGACGTG 
ACTATTGGCC CGCGTCTGTT CCTCTCCTGC CCGCTGGACT TGAAGGACTC GCAGGTGTGG 
TTCACCGACA TTTGGAACTA TCATCTGTCC CCCTATCTGG TCGAGGCTGT GCGCGAGGGA 
GTGCAGCTCT ATGGCCGCCG GGGCGGAGCC TGGAACGATC CTTCTGTCTT CATACGCAAC 
TCCTATCCCT GGCCCTATGG GCCCGACTCA GTGCCACCGC TGCGACAGAT CAATGCAGAG 
GATGTGGGCC TAGAAGGCGT CGCATTGACA AACGGGGAGA ACCAGGATCC TCTGCTCAAT 
ATGCTGATGC GCCTGCAGGA GGCTGCCAAC TACTCGGAGG CGCAGGATCA GGAGTCGGAC 
TGCGCCAGTC TGGACTCCAA CGTGACGCCG GACAGCTCTG CGGGCGCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA28818 ( XM_015181240.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036726.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28818), mRNA.

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CloneID	ODe18638
Clone ID Related Accession (Same CDS sequence)	XM_015181240.1
Accession Version	XM_015181240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATA CATCGTCCTC ATCATCGGGA TCGCATGGTG GGGGCAAGAG TTCACGGAAA 
TGTCGAAGTC TACCCAACTC TCCGGATGTC AGAAGACAAA TAGCCCCCGC CTATAAGGTC 
AATGGCGGCA CTGCCATACC ACTGCCGGCC ACCGTTATGG TGCAGCGTCG CTGTCCACCG 
GATAAAGTGC GTCCACTGCC CCCGACACCA AATCATACGC CATCCATACC GGGACTGGGC 
AAGAGCGGAA GTGATTTCAA TACGAGCCGT CCAAACAGTC CGCCCACCAG CAATCATACA 
ATCCAAAGCC TCAAGAGTGG CAATAACAAC AGCCTACGTC CACCGAGTAT CAAGAGCAAC 
CACCAAAGCG GAATACCCTC CCCCAGTAGC CCCCAGACCA CGACGACGGC GGCTGCGGCA 
GCTCCCCAGA AGCACTCGAT GCTGGACAAG CTGAAGCTCT TCAACAAGGA GAAGCAGCAG 
AATGCAGTGA ATGCGGCGGC GGCGGCGGCG GCGGCGGCCA ACAAATCGCA GATCCAGCAG 
CAACAGTCGA AGCGCACCTC CTCCTCGTCG GGGTTTAGCT CGGCGCGTTC CGAGCGCTCC 
GACTCCAGTC TGAGCCTCAA CGATGGCCAC AACTCGTCGC AGCTGAAGCC CCCCAGCATC 
AGTGTGTCCT CGCAGAAGCC CCAACAGAAG CACAACACGA AGCAGTCGAA GCTGCTGGCG 
GCACAACAGA AGAAGGAGCA GACAAAGTCG AAGCTGGACA AAAAGGAAAA GAGTCCGGCC 
CGCTCGCTGC ACAAGGAAGA GTCCGGGAAT GAGTCGCGCA GCTCCACGAT GGGCAGGGCG 
GGCAAGAGCT CCCTGGCCAG AGGAGGCCTC GGTCTTGGCC TCACGGTGGA GAAGAACACA 
CTGAAGACAT CGTCCAAGTC CTCGCTGCAC TCCAAGTCCG AGTCGAAGAG CTCTCTAAAG 
GGTGTCGGAG CCCAGCAGCA GCAGCAGATG TTGCACAGTC CGAGTGGCAC GAGTCTGCCC 
AAACCCATTG CGGCGATCAA GGGCACCAGC AAGCTGCCGG CCCTAGCGGG AGGAGAGCTC 
CTGAAGCGGG AAACCAGCGA CATATCAGCG GCCAATCAGG CAACGCAGAC CACCCAGAAC 
ATATCCACCA TATCCGTGCA GGAATCACAG CAGCAGCCAC TGCCTCTGAG CACCACTACA 
CCACATATCC TTAAGGGTCC AGCGCCACCG CCGTACTACG CGAATAGCAG TCCACCCTCA 
CACATATCAC AAGGACATCC CGGCATGGGA GGATACCTCA GTGAGCCCAG CACTCCCCAG 
CACCAGCAGG GGATCTACGC GAGCAGCAGC GGCAGTCGTC TGCCACCGCC CAAGACGGCG 
CTGAGTGCCC CCCGGAAACT GGAGTACAAT GCAGGACCCC ATATCCTGTC ATCGCCTCCC 
CATCGAGGGG CAGGTCTGCA GCGTCCGATG CCCAACTCTG CCCCGAATAC ACCCACTGCA 
TCGCCGAACA AGTTCCACAC GGTACCCTCG AAGATTGTGG GCACCATTTA CGAGTCGAAG 
GAGGAGCAGC AGCAGCAGCC CCAGCAGACA GGCGGATGTT CCGCCGGAGC CTCAGTGCTG 
CCCATGAGAC CCCTCCTGCG GGGCTACAAC TCCCATGTGA CGCTGCCGAC ACGTGGAGCA 
CGCGGTGGCC ACCACCATCC GCATCCGCCT CAGTCGTATC TGGATTTCTG CGAGTCGGAT 
CTGGGGCAGG GCTACTGCAG CGATGGCGAC GCCCTGAGGA TTGGCAGCTC TCCGGCCACC 
AGTCGCTATC AAGACATCGA CAATGGCTAT CTGTCGGAGG GGAGCAGCGG CCTGAATGGA 
TCGATCGGCG GATCCACTGG TGGTGCGGTG TCCCACGGAA AACACTTTCT CAGGATGATG 
AGGGCCAGAA CACCGCTGCC AACAACCATT GAAGAGAGAA TCCGCAATAG TCGAGGCTCT 
CTAGACAGCA TTGGAACCGC CGCAAACGGC AGCTCTGCGG CCAGTCAGGC CAGCTCCAGC 
GGCGGCTCCA CCACCCACAG CAGCAGCAAC AGCAACAGCG CCGGCACCAC CGCCAACCAC 
AACAACAACC ACATCAACAA CAACAACGGA GGGAAAATGG ATGGCAGTCC ACACCATCGT 
CCGGGATCAC GCAATGGAAG GGATAACTGG AGCAAAATGC CAGAGCCGCT CAACGGCCAG 
AAGGGGGACA AATCGTCGGA GAAGTCATCG CCATCGCGCA GGAGCATGGG CGGTGGCAGC 
GGGGGATCCT CCAAGCAGGG TTCGCCCTCG TCTTCCTCCA GGACCAAGGG AGTCCCACCG 
AGTTTCGGGT ATGTGAAGAG GGCCAATGGC AGCATGTCCT CCACTGCAGA GCAGCAGAAT 
ATTGTCATGA TGATGGCAGC CGGAGGCGGA GGAGGACCGC AGCAGGTCAA TGGATTGCCC 
TGCGGCAGGA CCGCCCATGT GTCGGCGGTG CCAAGGACAG CAAATGGTCG AAAGATTGCA 
CCCGGCACTC AGACATTGCC CAATGATATG AACAAGCTAC CACCAAACAC ACAGTGTCGC 
AGCTTCTCAC TGACGGGACC CACAGCGGCA CAATTGAGTC AATCGATACG CGAGCGTTTG 
GCCACAGGAT CGCACAGTTT GCCCAAACCG GGCAGCGATA TGCATGTCTT TCAGCATAGA 
ATGTCCAATC GCCCTGGCAC CCGTCACGAT GGCTCTCTCT CCGACACCCA GACCTATGCC 
GAGGTCAAGC CCGAATACAG TTCGTATGCC ATGTGGCTGA AGCACAGCAA TACGGCCGGA 
TCACGTCTCT CGGATGGCGA TGCCATTGAG CAGCTGCAGA TTGGATCGCC AGCGATGACC 
CGTCACGGGC ACAAGATGAT CCACAATCGA TCGGGAGGAG GACCCCCTGG TGGACAAATG 
CCCATGCAGG GAAATGAGTC GCCCTACGTG CAGAGTCCTC GCATGAATCG GAGCAATAGC 
ATCAGCTACT TGCAAGAAAG GACATATCCA AGATCCACAA AATCGGAGAA AATGTATCCC 
TCGATGCTGA GCCGCGCTGG GGACGTGGAA ATCGAGCCGT ACTATTGCCT GCCGGTGGGC 
ACCAACGGAG TGCTGAGCGC CCAAATGGCA GCTGCTGTGG CGGCCCAGGC CCAAGCCCAA 
GCACAGGCCA CGCAGGGAAA TTCTGGCAAC AATGTGGTGG GTGGTTCCGT GGCCTGGAGC 
CAGCCCACAT CGCCCACGCC CCTCAATCGA GGCCCCTTTG CGGCGTCAGT GGCAGCAGCG 
GCAGCAGCAG CAGCTGCTGC AGGGGTTCTA TCGCCCACTC ACGGAACGAC CGCAGGAGCC 
ACAGGGATGG TTGGTGGCAT GGGAATGGGA CCCGGACTCG GCTCCGGTCT AACTGGCGGA 
CATCGCCTCA CATATCCCAA GAAGAACGAC GAAGTGCACG GCAGTGCCGC ATCCCTGCTG 
TCGGGCGGCA GTTCCCTGTA CGGATCCGCG GAGGAGCGGC AGGCCCACGA GATACGGAGA 
TTGAAGCGCG AACTGCAGGA TGCACGCGAC CAGGTGCTCA GCCTAAGCAG CCAGCTGTCA 
ACCAATGCCC ATGTGGTGAC GGCGTTTGAA CAGTCGCTGA CCAACATGAC CAATCGCCTG 
CAGCAGCTGA CGGCGACGTC GGAGCGCAAG GACGGAGAGC TGACGGACAT GCGGCAGACC 
ATCGAGCTGC TGAGGAAGCA GTCCATCCAG GCGGGCCTCA CCACCGCGCA TATGCAGAGC 
ATGGGACAGA ATCAGAATCA CCAGCTGCAG TCGCAGCCCG GCGACCAGAA GCCACCGCCA 
GGACCGGGGG GCAGCAGGTC CGCCAGCAAT GTCAATGGCA ACGGCACTGG TAATGGCAAT 
GGCAACGGCA TACCCATCGG ATTGGGAATG GTGCGGCAGC ACAGCACGGA CTCGATGTGC 
TCCTTGAATT CCATTAGCTC GGGATGCTCG GCGGCGCAGG ACAAGAACAA GGCCAACAAG 
AAGAAGGGGT GGCTGCGCAG CAGCTTCACC AAGGCCTTCT CGCGGAACGC CAAGATCTCG 
AAGACCAGTC GCCATGTGGG ACACCACCAC CACCACAATC ATCACGGACA GGAGCACAGC 
TCCGGGAAGA CACCGCTCCC GAATGGTCAT GGCGAGCCGC TGGGTCTGGC GGCCCTGGCC 
ACCCAACCGG CGCCTCCTTT GCCTAACAGC AAGCAGGGCA GTCCCGCCAA GACCCTGACG 
CTGATCGACA ACGCCAAGCC CATCGATGCC ATCGACCAGG AGGACCAGCA GGTGGTGGAG 
GACCTCAAGA AGCAGCTGCG CGAGAAGGAT CTCGTCCTCA CAGACATTCG GCTAGAGGCC 
CTTAGCTCCG CCTCGCAGCT GGAGAGCCTC AAGGACATGA TGAACAAGAT GCGGTCCGAG 
ATGATGTCCC TCAAGCAGAA CAACGAGCGT CTGCAGAAGC TGGTGACCAC CCGCTCCCTG 
GCCGGCTCCG AGGTGAGCCT CGGACAGGCG ATCAGCCCCA ACGGATCGGT GGCCGGCAGC 
TCTGAGGTCT CGCGCCGCTA CTCGCTGGCC GACGGCAGTG CGCGACCGCC CATGGAGCTG 
CCACCACGCC TCAGCGAGGA GTTGGAGGAG GAGTGCATGC CCCCAGCACC GGCGCCAGAG 
CCCCCACCAC CGCCAGCACC AAACACCGCC GCTGTGGCGC CTCTGAGTCC CAGCACACAC 
ATTGATCTGA CGCCTCCACC ACCGGCCCTG GAGACGGCGC CGCTGGCCAG CCCCGTGCAC 
ATGCCCAGCG CCGTCGAGGA TCTGCCCGAC GTGTGCGACG GCAAGAAGAT TGCCATCGCC 
TGCTACCTGG GACAGCCGGA GTCGTTTGCC AAGTACTGCG AGGAGCTGCA GGAGCTGGAT 
GGCTTCTATG CCAATGGCTC GATCATCGCC AGCCACGAGG CCGACCCCCA GGCGCAGGGC 
GAGCGCAAGG TGAGCTTTGC AGCCGCCAGC TCAAACGAGT TCATCGTCGC CTGCACCTAC 
ATCTCGGGCA AGACGACCTG GCAGAATCTC GACTACATTG TGCGCAAGAC CTTTAAGGAC 
TATGTGGCTC GCATCGATCC AGGCACCAAT CTGGGCCTGA ACACGGACTC CATCACGTCG 
TACCATCTGG GAGAGGCCAA GCGCGGGCCG GAGATGGGCT TCCCCGAGCT CCTGCCCTGC 
GGCTACATTG TGGGCAGTGT CCGGACGCTG TATATCTGCC TGCAGGGCGT TGGGAGTCTG 
GCCTTCGACA GCCTCATTCC ACGCAGCATT GTGCACCGCT ACATCAGTCT CCTCACGGAG 
CACAGGCGGC TCATCCTGTG CGGTCCCAGC GGCACTGGGA AGTCCTATCT GGCGCGTCGC 
CTAGCCGAGT TCCTAGTGGC GCGTGCGGGA CGGGGAAATC CCTCAGAGGC CATTGCCACG 
TTCAACGTGG ACCACAAATC CTCGAAGGAT CTGCGGCAGT ATCTGGGCCA CATTGCCGAG 
CAGGCGGCCA TTGCAAATGG CGCTTCGGAG CTGCCTTCTG TGATCATTCT GGACAACTTG 
CACCATGCCT CTGCCCTGGG CGATGTCTTC TCCTGCCTGC TGAGCGCCGG GCCGGCCAAC 
AAATTGCCCT GCATTATTGG CACCATGTCG CAGGCCACCT GCAACACGAC CAATCTTCAG 
CTGCACCACA ATTTCCGATG GGTCCTTACG GCCAATCATA TGGAGCCCGT CAAGGGCTTC 
CTTGGACGCT TCCTGCGGCG GCGACTGTTC CAGCTGGAGC TGCAGACGCA GCATGCCCAG 
CCAGAGCTGG CGTCGGTCTT GGCCTGGCTG CCCTCCGTCT GGCAGCACAT CAATCGTTTC 
CTGGAAGTCC ACAGCTCCAG TGACGTGACT ATTGGCCCGC GTCTGTTCCT CTCCTGCCCG 
CTGGACTTGA AGGACTCGCA GGTGTGGTTC ACCGACATTT GGAACTATCA TCTGTCCCCC 
TATCTGGTCG AGGCTGTGCG CGAGGGAGTG CAGCTCTATG GCCGCCGGGG CGGAGCCTGG 
AACGATCCTT CTGTCTTCAT ACGCAACTCC TATCCCTGGC CCTATGGGCC CGACTCAGTG 
CCACCGCTGC GACAGATCAA TGCAGAGGAT GTGGGCCTAG AAGGCGTCGC ATTGACAAAC 
GGGGAGAACC AGGATCCTCT GCTCAATATG CTGATGCGCC TGCAGGAGGC TGCCAACTAC 
TCGGAGGCGC AGGATCAGGA GTCGGACTGC GCCAGTCTGG ACTCCAACGT GACGCCGGAC 
AGCTCTGCGG GCGCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036726.1
CDS	121..6441
Translation	MGNTSSSSSGSHGGGKSSRKCRSLPNSPDVRRQIAPAYKVNGGTAIPLPATVMVQRRCPPDKVRPLPPTPNHTPSIPGLGKSGSDFNTSRPNSPPTSNHTIQSLKSGNNNSLRPPSIKSNHQSGIPSPSSPQTTTTAAAAAPQKHSMLDKLKLFNKEKQQNAVNAAAAAAAAANKSQIQQQQSKRTSSSSGFSSARSERSDSSLSLNDGHNSSQLKPPSISVSSQKPQQKHNTKQSKLLAAQQKKEQTKSKLDKKEKSPARSLHKEESGNESRSSTMGRAGKSSLARGGLGLGLTVEKNTLKTSSKSSLHSKSESKSSLKGVGAQQQQQMLHSPSGTSLPKPIAAIKGTSKLPALAGGELLKRETSDISAANQATQTTQNISTISVQESQQQPLPLSTTTPHILKGPAPPPYYANSSPPSHISQGHPGMGGYLSEPSTPQHQQGIYASSSGSRLPPPKTALSAPRKLEYNAGPHILSSPPHRGAGLQRPMPNSAPNTPTASPNKFHTVPSKIVGTIYESKEEQQQQPQQTGGCSAGASVLPMRPLLRGYNSHVTLPTRGARGGHHHPHPPQSYLDFCESDLGQGYCSDGDALRIGSSPATSRYQDIDNGYLSEGSSGLNGSIGGSTGGAVSHGKHFLRMMRARTPLPTTIEERIRNSRGSLDSIGTAANGSSAASQASSSGGSTTHSSSNSNSAGTTANHNNNHINNNNGGKMDGSPHHRPGSRNGRDNWSKMPEPLNGQKGDKSSEKSSPSRRSMGGGSGGSSKQGSPSSSSRTKGVPPSFGYVKRANGSMSSTAEQQNIVMMMAAGGGGGPQQVNGLPCGRTAHVSAVPRTANGRKIAPGTQTLPNDMNKLPPNTQCRSFSLTGPTAAQLSQSIRERLATGSHSLPKPGSDMHVFQHRMSNRPGTRHDGSLSDTQTYAEVKPEYSSYAMWLKHSNTAGSRLSDGDAIEQLQIGSPAMTRHGHKMIHNRSGGGPPGGQMPMQGNESPYVQSPRMNRSNSISYLQERTYPRSTKSEKMYPSMLSRAGDVEIEPYYCLPVGTNGVLSAQMAAAVAAQAQAQAQATQGNSGNNVVGGSVAWSQPTSPTPLNRGPFAASVAAAAAAAAAAGVLSPTHGTTAGATGMVGGMGMGPGLGSGLTGGHRLTYPKKNDEVHGSAASLLSGGSSLYGSAEERQAHEIRRLKRELQDARDQVLSLSSQLSTNAHVVTAFEQSLTNMTNRLQQLTATSERKDGELTDMRQTIELLRKQSIQAGLTTAHMQSMGQNQNHQLQSQPGDQKPPPGPGGSRSASNVNGNGTGNGNGNGIPIGLGMVRQHSTDSMCSLNSISSGCSAAQDKNKANKKKGWLRSSFTKAFSRNAKISKTSRHVGHHHHHNHHGQEHSSGKTPLPNGHGEPLGLAALATQPAPPLPNSKQGSPAKTLTLIDNAKPIDAIDQEDQQVVEDLKKQLREKDLVLTDIRLEALSSASQLESLKDMMNKMRSEMMSLKQNNERLQKLVTTRSLAGSEVSLGQAISPNGSVAGSSEVSRRYSLADGSARPPMELPPRLSEELEEECMPPAPAPEPPPPPAPNTAAVAPLSPSTHIDLTPPPPALETAPLASPVHMPSAVEDLPDVCDGKKIAIACYLGQPESFAKYCEELQELDGFYANGSIIASHEADPQAQGERKVSFAAASSNEFIVACTYISGKTTWQNLDYIVRKTFKDYVARIDPGTNLGLNTDSITSYHLGEAKRGPEMGFPELLPCGYIVGSVRTLYICLQGVGSLAFDSLIPRSIVHRYISLLTEHRRLILCGPSGTGKSYLARRLAEFLVARAGRGNPSEAIATFNVDHKSSKDLRQYLGHIAEQAAIANGASELPSVIILDNLHHASALGDVFSCLLSAGPANKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLGRFLRRRLFQLELQTQHAQPELASVLAWLPSVWQHINRFLEVHSSSDVTIGPRLFLSCPLDLKDSQVWFTDIWNYHLSPYLVEAVREGVQLYGRRGGAWNDPSVFIRNSYPWPYGPDSVPPLRQINAEDVGLEGVALTNGENQDPLLNMLMRLQEAANYSEAQDQESDCASLDSNVTPDSSAGAE

Target ORF information:

RefSeq Version	XM_015181240.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28818), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181240.1

ORF Insert Sequence:

ATGGGCAATA CATCGTCCTC ATCATCGGGA TCGCATGGTG GGGGCAAGAG TTCACGGAAA 
TGTCGAAGTC TACCCAACTC TCCGGATGTC AGAAGACAAA TAGCCCCCGC CTATAAGGTC 
AATGGCGGCA CTGCCATACC ACTGCCGGCC ACCGTTATGG TGCAGCGTCG CTGTCCACCG 
GATAAAGTGC GTCCACTGCC CCCGACACCA AATCATACGC CATCCATACC GGGACTGGGC 
AAGAGCGGAA GTGATTTCAA TACGAGCCGT CCAAACAGTC CGCCCACCAG CAATCATACA 
ATCCAAAGCC TCAAGAGTGG CAATAACAAC AGCCTACGTC CACCGAGTAT CAAGAGCAAC 
CACCAAAGCG GAATACCCTC CCCCAGTAGC CCCCAGACCA CGACGACGGC GGCTGCGGCA 
GCTCCCCAGA AGCACTCGAT GCTGGACAAG CTGAAGCTCT TCAACAAGGA GAAGCAGCAG 
AATGCAGTGA ATGCGGCGGC GGCGGCGGCG GCGGCGGCCA ACAAATCGCA GATCCAGCAG 
CAACAGTCGA AGCGCACCTC CTCCTCGTCG GGGTTTAGCT CGGCGCGTTC CGAGCGCTCC 
GACTCCAGTC TGAGCCTCAA CGATGGCCAC AACTCGTCGC AGCTGAAGCC CCCCAGCATC 
AGTGTGTCCT CGCAGAAGCC CCAACAGAAG CACAACACGA AGCAGTCGAA GCTGCTGGCG 
GCACAACAGA AGAAGGAGCA GACAAAGTCG AAGCTGGACA AAAAGGAAAA GAGTCCGGCC 
CGCTCGCTGC ACAAGGAAGA GTCCGGGAAT GAGTCGCGCA GCTCCACGAT GGGCAGGGCG 
GGCAAGAGCT CCCTGGCCAG AGGAGGCCTC GGTCTTGGCC TCACGGTGGA GAAGAACACA 
CTGAAGACAT CGTCCAAGTC CTCGCTGCAC TCCAAGTCCG AGTCGAAGAG CTCTCTAAAG 
GGTGTCGGAG CCCAGCAGCA GCAGCAGATG TTGCACAGTC CGAGTGGCAC GAGTCTGCCC 
AAACCCATTG CGGCGATCAA GGGCACCAGC AAGCTGCCGG CCCTAGCGGG AGGAGAGCTC 
CTGAAGCGGG AAACCAGCGA CATATCAGCG GCCAATCAGG CAACGCAGAC CACCCAGAAC 
ATATCCACCA TATCCGTGCA GGAATCACAG CAGCAGCCAC TGCCTCTGAG CACCACTACA 
CCACATATCC TTAAGGGTCC AGCGCCACCG CCGTACTACG CGAATAGCAG TCCACCCTCA 
CACATATCAC AAGGACATCC CGGCATGGGA GGATACCTCA GTGAGCCCAG CACTCCCCAG 
CACCAGCAGG GGATCTACGC GAGCAGCAGC GGCAGTCGTC TGCCACCGCC CAAGACGGCG 
CTGAGTGCCC CCCGGAAACT GGAGTACAAT GCAGGACCCC ATATCCTGTC ATCGCCTCCC 
CATCGAGGGG CAGGTCTGCA GCGTCCGATG CCCAACTCTG CCCCGAATAC ACCCACTGCA 
TCGCCGAACA AGTTCCACAC GGTACCCTCG AAGATTGTGG GCACCATTTA CGAGTCGAAG 
GAGGAGCAGC AGCAGCAGCC CCAGCAGACA GGCGGATGTT CCGCCGGAGC CTCAGTGCTG 
CCCATGAGAC CCCTCCTGCG GGGCTACAAC TCCCATGTGA CGCTGCCGAC ACGTGGAGCA 
CGCGGTGGCC ACCACCATCC GCATCCGCCT CAGTCGTATC TGGATTTCTG CGAGTCGGAT 
CTGGGGCAGG GCTACTGCAG CGATGGCGAC GCCCTGAGGA TTGGCAGCTC TCCGGCCACC 
AGTCGCTATC AAGACATCGA CAATGGCTAT CTGTCGGAGG GGAGCAGCGG CCTGAATGGA 
TCGATCGGCG GATCCACTGG TGGTGCGGTG TCCCACGGAA AACACTTTCT CAGGATGATG 
AGGGCCAGAA CACCGCTGCC AACAACCATT GAAGAGAGAA TCCGCAATAG TCGAGGCTCT 
CTAGACAGCA TTGGAACCGC CGCAAACGGC AGCTCTGCGG CCAGTCAGGC CAGCTCCAGC 
GGCGGCTCCA CCACCCACAG CAGCAGCAAC AGCAACAGCG CCGGCACCAC CGCCAACCAC 
AACAACAACC ACATCAACAA CAACAACGGA GGGAAAATGG ATGGCAGTCC ACACCATCGT 
CCGGGATCAC GCAATGGAAG GGATAACTGG AGCAAAATGC CAGAGCCGCT CAACGGCCAG 
AAGGGGGACA AATCGTCGGA GAAGTCATCG CCATCGCGCA GGAGCATGGG CGGTGGCAGC 
GGGGGATCCT CCAAGCAGGG TTCGCCCTCG TCTTCCTCCA GGACCAAGGG AGTCCCACCG 
AGTTTCGGGT ATGTGAAGAG GGCCAATGGC AGCATGTCCT CCACTGCAGA GCAGCAGAAT 
ATTGTCATGA TGATGGCAGC CGGAGGCGGA GGAGGACCGC AGCAGGTCAA TGGATTGCCC 
TGCGGCAGGA CCGCCCATGT GTCGGCGGTG CCAAGGACAG CAAATGGTCG AAAGATTGCA 
CCCGGCACTC AGACATTGCC CAATGATATG AACAAGCTAC CACCAAACAC ACAGTGTCGC 
AGCTTCTCAC TGACGGGACC CACAGCGGCA CAATTGAGTC AATCGATACG CGAGCGTTTG 
GCCACAGGAT CGCACAGTTT GCCCAAACCG GGCAGCGATA TGCATGTCTT TCAGCATAGA 
ATGTCCAATC GCCCTGGCAC CCGTCACGAT GGCTCTCTCT CCGACACCCA GACCTATGCC 
GAGGTCAAGC CCGAATACAG TTCGTATGCC ATGTGGCTGA AGCACAGCAA TACGGCCGGA 
TCACGTCTCT CGGATGGCGA TGCCATTGAG CAGCTGCAGA TTGGATCGCC AGCGATGACC 
CGTCACGGGC ACAAGATGAT CCACAATCGA TCGGGAGGAG GACCCCCTGG TGGACAAATG 
CCCATGCAGG GAAATGAGTC GCCCTACGTG CAGAGTCCTC GCATGAATCG GAGCAATAGC 
ATCAGCTACT TGCAAGAAAG GACATATCCA AGATCCACAA AATCGGAGAA AATGTATCCC 
TCGATGCTGA GCCGCGCTGG GGACGTGGAA ATCGAGCCGT ACTATTGCCT GCCGGTGGGC 
ACCAACGGAG TGCTGAGCGC CCAAATGGCA GCTGCTGTGG CGGCCCAGGC CCAAGCCCAA 
GCACAGGCCA CGCAGGGAAA TTCTGGCAAC AATGTGGTGG GTGGTTCCGT GGCCTGGAGC 
CAGCCCACAT CGCCCACGCC CCTCAATCGA GGCCCCTTTG CGGCGTCAGT GGCAGCAGCG 
GCAGCAGCAG CAGCTGCTGC AGGGGTTCTA TCGCCCACTC ACGGAACGAC CGCAGGAGCC 
ACAGGGATGG TTGGTGGCAT GGGAATGGGA CCCGGACTCG GCTCCGGTCT AACTGGCGGA 
CATCGCCTCA CATATCCCAA GAAGAACGAC GAAGTGCACG GCAGTGCCGC ATCCCTGCTG 
TCGGGCGGCA GTTCCCTGTA CGGATCCGCG GAGGAGCGGC AGGCCCACGA GATACGGAGA 
TTGAAGCGCG AACTGCAGGA TGCACGCGAC CAGGTGCTCA GCCTAAGCAG CCAGCTGTCA 
ACCAATGCCC ATGTGGTGAC GGCGTTTGAA CAGTCGCTGA CCAACATGAC CAATCGCCTG 
CAGCAGCTGA CGGCGACGTC GGAGCGCAAG GACGGAGAGC TGACGGACAT GCGGCAGACC 
ATCGAGCTGC TGAGGAAGCA GTCCATCCAG GCGGGCCTCA CCACCGCGCA TATGCAGAGC 
ATGGGACAGA ATCAGAATCA CCAGCTGCAG TCGCAGCCCG GCGACCAGAA GCCACCGCCA 
GGACCGGGGG GCAGCAGGTC CGCCAGCAAT GTCAATGGCA ACGGCACTGG TAATGGCAAT 
GGCAACGGCA TACCCATCGG ATTGGGAATG GTGCGGCAGC ACAGCACGGA CTCGATGTGC 
TCCTTGAATT CCATTAGCTC GGGATGCTCG GCGGCGCAGG ACAAGAACAA GGCCAACAAG 
AAGAAGGGGT GGCTGCGCAG CAGCTTCACC AAGGCCTTCT CGCGGAACGC CAAGATCTCG 
AAGACCAGTC GCCATGTGGG ACACCACCAC CACCACAATC ATCACGGACA GGAGCACAGC 
TCCGGGAAGA CACCGCTCCC GAATGGTCAT GGCGAGCCGC TGGGTCTGGC GGCCCTGGCC 
ACCCAACCGG CGCCTCCTTT GCCTAACAGC AAGCAGGGCA GTCCCGCCAA GACCCTGACG 
CTGATCGACA ACGCCAAGCC CATCGATGCC ATCGACCAGG AGGACCAGCA GGTGGTGGAG 
GACCTCAAGA AGCAGCTGCG CGAGAAGGAT CTCGTCCTCA CAGACATTCG GCTAGAGGCC 
CTTAGCTCCG CCTCGCAGCT GGAGAGCCTC AAGGACATGA TGAACAAGAT GCGGTCCGAG 
ATGATGTCCC TCAAGCAGAA CAACGAGCGT CTGCAGAAGC TGGTGACCAC CCGCTCCCTG 
GCCGGCTCCG AGGTGAGCCT CGGACAGGCG ATCAGCCCCA ACGGATCGGT GGCCGGCAGC 
TCTGAGGTCT CGCGCCGCTA CTCGCTGGCC GACGGCAGTG CGCGACCGCC CATGGAGCTG 
CCACCACGCC TCAGCGAGGA GTTGGAGGAG GAGTGCATGC CCCCAGCACC GGCGCCAGAG 
CCCCCACCAC CGCCAGCACC AAACACCGCC GCTGTGGCGC CTCTGAGTCC CAGCACACAC 
ATTGATCTGA CGCCTCCACC ACCGGCCCTG GAGACGGCGC CGCTGGCCAG CCCCGTGCAC 
ATGCCCAGCG CCGTCGAGGA TCTGCCCGAC GTGTGCGACG GCAAGAAGAT TGCCATCGCC 
TGCTACCTGG GACAGCCGGA GTCGTTTGCC AAGTACTGCG AGGAGCTGCA GGAGCTGGAT 
GGCTTCTATG CCAATGGCTC GATCATCGCC AGCCACGAGG CCGACCCCCA GGCGCAGGGC 
GAGCGCAAGG TGAGCTTTGC AGCCGCCAGC TCAAACGAGT TCATCGTCGC CTGCACCTAC 
ATCTCGGGCA AGACGACCTG GCAGAATCTC GACTACATTG TGCGCAAGAC CTTTAAGGAC 
TATGTGGCTC GCATCGATCC AGGCACCAAT CTGGGCCTGA ACACGGACTC CATCACGTCG 
TACCATCTGG GAGAGGCCAA GCGCGGGCCG GAGATGGGCT TCCCCGAGCT CCTGCCCTGC 
GGCTACATTG TGGGCAGTGT CCGGACGCTG TATATCTGCC TGCAGGGCGT TGGGAGTCTG 
GCCTTCGACA GCCTCATTCC ACGCAGCATT GTGCACCGCT ACATCAGTCT CCTCACGGAG 
CACAGGCGGC TCATCCTGTG CGGTCCCAGC GGCACTGGGA AGTCCTATCT GGCGCGTCGC 
CTAGCCGAGT TCCTAGTGGC GCGTGCGGGA CGGGGAAATC CCTCAGAGGC CATTGCCACG 
TTCAACGTGG ACCACAAATC CTCGAAGGAT CTGCGGCAGT ATCTGGGCCA CATTGCCGAG 
CAGGCGGCCA TTGCAAATGG CGCTTCGGAG CTGCCTTCTG TGATCATTCT GGACAACTTG 
CACCATGCCT CTGCCCTGGG CGATGTCTTC TCCTGCCTGC TGAGCGCCGG GCCGGCCAAC 
AAATTGCCCT GCATTATTGG CACCATGTCG CAGGCCACCT GCAACACGAC CAATCTTCAG 
CTGCACCACA ATTTCCGATG GGTCCTTACG GCCAATCATA TGGAGCCCGT CAAGGGCTTC 
CTTGGACGCT TCCTGCGGCG GCGACTGTTC CAGCTGGAGC TGCAGACGCA GCATGCCCAG 
CCAGAGCTGG CGTCGGTCTT GGCCTGGCTG CCCTCCGTCT GGCAGCACAT CAATCGTTTC 
CTGGAAGTCC ACAGCTCCAG TGACGTGACT ATTGGCCCGC GTCTGTTCCT CTCCTGCCCG 
CTGGACTTGA AGGACTCGCA GGTGTGGTTC ACCGACATTT GGAACTATCA TCTGTCCCCC 
TATCTGGTCG AGGCTGTGCG CGAGGGAGTG CAGCTCTATG GCCGCCGGGG CGGAGCCTGG 
AACGATCCTT CTGTCTTCAT ACGCAACTCC TATCCCTGGC CCTATGGGCC CGACTCAGTG 
CCACCGCTGC GACAGATCAA TGCAGAGGAT GTGGGCCTAG AAGGCGTCGC ATTGACAAAC 
GGGGAGAACC AGGATCCTCT GCTCAATATG CTGATGCGCC TGCAGGAGGC TGCCAACTAC 
TCGGAGGCGC AGGATCAGGA GTCGGACTGC GCCAGTCTGG ACTCCAACGT GACGCCGGAC 
AGCTCTGCGG GCGCCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA28818 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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