Dpse\GA23359 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA23359
Entrez Gene ID	6900879
Full Name	GA23359 gene product from transcript GA23359-RB
Synonyms	GA23359,dpse_GLEANR_12040
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002135200.2	XP_002135236.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA23359 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA23359 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25970	XM_002135200.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23359), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA23359 ( XM_002135200.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002135236.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23359), mRNA.

Price & Availability↑

CloneID	ODe25970
Clone ID Related Accession (Same CDS sequence)	XM_002135200.2
Accession Version	XM_002135200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_002135200.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATTG AGCCGAAGGA GTTGCTGTTG CCGTCGACGT TTTGGATTGC CGAAATGTAC 
TCCAGGTACT TCGTGCTACA GAAACGTCGC GGCCACGGCG AGTCGCGCGG CTTTGGGTCC 
ATGCTGGTGG GCACCTACGA CAGTGTTTGG AACACCAAGC CGGCTCCGTA TCGCATTGTA 
CACCGGACAC CGTCCTCGGA AGTTTCTTAC GAAATCGCCA TTGGAATAAC GCAGGATGAG 
ATTGCAAAGG ACTGGGAATG GCTGCAGGCG AATCTCTTCA ATGTGCTCAA CGAAATGGAG 
AACGAGGACG AGGTTACAAA CTTTACTATA TGCAAAATCA AATCGCTCTA CACACAAAAT 
AATCAAGATG AAACTGGCGA AACGGCCGAC TTCAAGGTGA TGACCTCTCA GTTTAGACAA 
ACCTTCAAAA TGCCGGAGGA AGAGCGTCTG GTCAACTCCT ACTCGGCCAC ATATGTGAAG 
AACAAGATAC CGCGTCAGGG TCAGCTATAC ATCTCTTTGA ATCACGTCTG CTTCTACTCG 
TATATGCTGG GTCATGAGGT CAAGAGGATA ATACGCTTTG CCGAGCTGGA GGACATCAGT 
CGGAATGCAA ATACGATTTA CTTGAAGACC ACCAACAATA TGACATACAA TTTTACGCTG 
ATCTTCAATG CCGGCGAGGC ACATCTGCTG ATCGAGCAGC TCAACAAAAT GGCCATACAA 
CAGCTGATCC AGGATCCAGA GAGTCCAGTG GTCGATCATG ATCCGTCGAA CTTTGCCCGA 
CTGGGCGCTA AATCATCCAA GAAACCGGTT CTGCTCAGGG ATCTGACAGC CCGCCAGAAG 
TCCGAGGAGT TTCGCATTTA TTTCCGCTTA CCCCAAACGG AGATAATCGA TGGGAAGATC 
AAGGCAAACA TTTGGACACC CTACTCGAAG CGGTTTAATG CTGGCTACAT ATATTTGTCG 
CCCAATTTCT TTTGCTTTCG CAGCGATGTC AAGGACTTGG TTAGTGTGGT CATACCCATG 
AAAACAATAA AGAGTGTGGA GAAGAAGGAC GATGGGCAGC AGCGTTTTGA CAATCAAATT 
GTCATCATCA CGTCGGAGAA TGTGCCTTTC ATGTTTGCCC AGATCGTCGA TAGAGAGGTG 
CTGATCTCAA AAATAACAGA TCTACTCTCC CGCATTCATG TTCCCGTGAG TCGGGAACGT 
GCCAAGTACG ACATCTCCTG GAGCAAGCAG ACAGCCTTGA TGAACACATT CAAGACACAG 
TTCAGTTCAG ACATAATCAG GAATCAGGAG GAGAAGATGG TGCGTTGGGA GGCACATTTC 
CGTGACTTTG GACGCGGCAT TGGCATGTTC CGCACCACCG ATGTCATAAA TCTCATTGTG 
GAGGGCATTC CGGACCAGTT GCGTCAGGAG ATTTGGTTGA TCTTCTCCGG TGCCATTCAC 
GACAAGGAAA TGAATCCCGG ATTGTACGAG GATCTGGTGG AGAAGGCTGC CTGCATTAAG 
GATTGCTTTG CTCACGACGA AATCGACAGG GATCTGCCCC GTTCACTGCC CGAGCATCCG 
GCATTCCAGA GCACAGACGG CATTGGCGCA CTGAGGCGCG TCCTGCAAGC CTATGCCCTG 
CGCAATCCGC CCGTTAAGGG CTGCCAGGCC ATGAACATTG TCTCGTCGGG ATCCTTACTG 
TTTTGCGACG AAGAGAACGC CTTCTGGATG CTGGCCAGCC TGTGCGAGAA CCTCTTGCCG 
GACTACTACA AGGACAAGGT GGTGGGGGCA CAGATTGATC AGGGCGTGCT CAATGAACTG 
GTCGAAACTC ATCTGGCCGA TCTGCACGAG CATCTGGAGC AGCTGGGCGT CATCAAAATG 
ATCTCCATTT CGTGGTTCCT CACCATCTTC ATGAGCGTAA TCAGCTATGA GAGCTCGCTC 
CACATACTCG ACTGTTTCTT CTACGAGGGC GCCAAGATCA TCTTTATGGT GGCTTTGCAG 
ATCATCGAAT GGAATCGCGA CAAGCTGCTC AAGTGCCAGG ACGATGGCGA GGCCATGCTG 
GTGCTGCAAA ACTATCTGGA GGGCATTTAC AATCCCGACT ATCAAGTGCC GCCCTCCACG 
GACAAACGCA AGATGGAACG CTCACAGACG CAAACTGTGC AGACCCTCAT CCACGAGGCG 
TACACAAAAT TCGGCGAGGA ACTGACCCAG CAGCGCATCG AGGAGCTGCG CAACAAGCAC 
CGACGCCTGA CCATGCGTCA GTTTGACATT GACAACGAGA AGACCGTTGT CAAGGCCTAT 
TTACAGAATC CCTATTTCGA TCGAAACGAG CTGCACATGC TGCTCACCAT CATCCGAGAG 
GAAAAACACG CGTTGAAGTC CATGCAGCAG CAGCAGAAGG CACAATGCTC GCTGTCGGAG 
ACGCCGCAGC TGCTGCATCA TCAATCGCCC ACCAGGGTAC CGAATGACCC TGTCCTGGGT 
GGCAGTAATG GCAGATACGA GGCCTACTGG GTCAACTATG AGGTATTCCA CACACTCTTC 
ACAGAGCTAA CTCCCTGGCG GAAGTGTGTC AGCGTGGATC TTGGCGAAAA GTTGTTCAGG 
CTCACAGACA AGAAGGGTAC TGGATATCTG GATTTTGGAC AGCTTATCAA TGCCCTGGGC 
TTGGTTTGCT CCTCCAAGAG CATGGAGAAA CTGAAATTAC TTTATGTGTT GCATTTGCCG 
CCTCTGTTAT CCAAGGCGGA AATCGAACGA TCACGTCGTC CGCGTCCACG GACCAAAGAT 
GATGCCGAGG AGGCCTTTGA GGCGGAAGAT TTCTTTGACG ACGATGCTTC AGAGTCCATG 
GAGGCTTTGC CCTCGCCCTC GGATCATCAA TTTGATGCCG ATGACTATGC TCTGATCAGT 
GCCACACAGC ATTTGCATAA TCTGGCGGGT ATATCGGGCA ATACGTTCAT GGATCTATCA 
CGCAACACGC CCAATTTGTC GCTGAATTCC AACACCTCCT CGCTGGCGCA GCGCAATTCA 
ACATTCTATG TGGATCTGCC AGCAGCAGCA CTCCCGGATC TGGGAGCGGC ACGAATGACT 
CCGCCGCCGG GAGCGACTGC TGGAGCAGCC TCAGCTTCCG CGGCTGCTGC TGCTGCAGCC 
GATAAAATGC TTCTGAATGT CGAGACTATA TCGAATTTCT CACAGATCAG TGACCTGGTT 
GCTGCTACGC GACTGGAGCG TGCCGACTCG AATGCCACGG ATTCGAGAAG CCTGGGCAGC 
CTCGGCTATC TGCTCGACCA GCCCGATGAG GGGGCCTCGG GCTCGCATCA GACCATTCCG 
CATTTGCGCA AGGAGAACTT CCAGCTGCTG TGGCGCAGCA TTATCGAGAT CATGGGCTGT 
GTGCAGGACG AGGAGATGCG AATGGCATAT GAGAATCTGC TGGAGCTGGG AAACAGCAAC 
ATCAAAAGGA ACCCAGCCTA GAGAGGCTTT ACCCCAACTC AATTTGGCGG CAGCCGGCAA 
CCGGATAGCA ATGGCAACCC CACAACGCCC GCAGCAGAGC ACAGCAGCAC CACCCGTCTA 
TTTGTGGCAC TCGAGGAGGA GCTCCGTCAG GCCAGCGGGA AGCCCAGCAG CAGCAGGGGT 
AGCAGCAGTA GCAGCACAAA CACCTCCGAG GCCTGGCACA TATCCATCAA TCAATTTATA 
GCCACGGTTC TCACCGTGAA TACCATTGTA CAAGGTTTCC AAACACCCAT CCAAATAACC 
GAACAGGTCG AACAGCTCCA GAAGAAGCGA CGCAAATGTC TATCCACCAA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002135236.2
CDS	112..3888
Translation	MWIEPKELLLPSTFWIAEMYSRYFVLQKRRGHGESRGFGSMLVGTYDSVWNTKPAPYRIVHRTPSSEVSYEIAIGITQDEIAKDWEWLQANLFNVLNEMENEDEVTNFTICKIKSLYTQNNQDETGETADFKVMTSQFRQTFKMPEEERLVNSYSATYVKNKIPRQGQLYISLNHVCFYSYMLGHEVKRIIRFAELEDISRNANTIYLKTTNNMTYNFTLIFNAGEAHLLIEQLNKMAIQQLIQDPESPVVDHDPSNFARLGAKSSKKPVLLRDLTARQKSEEFRIYFRLPQTEIIDGKIKANIWTPYSKRFNAGYIYLSPNFFCFRSDVKDLVSVVIPMKTIKSVEKKDDGQQRFDNQIVIITSENVPFMFAQIVDREVLISKITDLLSRIHVPVSRERAKYDISWSKQTALMNTFKTQFSSDIIRNQEEKMVRWEAHFRDFGRGIGMFRTTDVINLIVEGIPDQLRQEIWLIFSGAIHDKEMNPGLYEDLVEKAACIKDCFAHDEIDRDLPRSLPEHPAFQSTDGIGALRRVLQAYALRNPPVKGCQAMNIVSSGSLLFCDEENAFWMLASLCENLLPDYYKDKVVGAQIDQGVLNELVETHLADLHEHLEQLGVIKMISISWFLTIFMSVISYESSLHILDCFFYEGAKIIFMVALQIIEWNRDKLLKCQDDGEAMLVLQNYLEGIYNPDYQVPPSTDKRKMERSQTQTVQTLIHEAYTKFGEELTQQRIEELRNKHRRLTMRQFDIDNEKTVVKAYLQNPYFDRNELHMLLTIIREEKHALKSMQQQQKAQCSLSETPQLLHHQSPTRVPNDPVLGGSNGRYEAYWVNYEVFHTLFTELTPWRKCVSVDLGEKLFRLTDKKGTGYLDFGQLINALGLVCSSKSMEKLKLLYVLHLPPLLSKAEIERSRRPRPRTKDDAEEAFEAEDFFDDDASESMEALPSPSDHQFDADDYALISATQHLHNLAGISGNTFMDLSRNTPNLSLNSNTSSLAQRNSTFYVDLPAAALPDLGAARMTPPPGATAGAASASAAAAAAADKMLLNVETISNFSQISDLVAATRLERADSNATDSRSLGSLGYLLDQPDEGASGSHQTIPHLRKENFQLLWRSIIEIMGCVQDEEMRMAYENLLELGNSNIKRNPA*RGFTPTQFGGSRQPDSNGNPTTPAAEHSSTTRLFVALEEELRQASGKPSSSRGSSSSSTNTSEAWHISINQFIATVLTVNTIVQGFQTPIQITEQVEQLQKKRRKCLSTNY

Target ORF information:

RefSeq Version	XM_002135200.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23359), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002135200.2

ORF Insert Sequence:

ATGTGGATTG AGCCGAAGGA GTTGCTGTTG CCGTCGACGT TTTGGATTGC CGAAATGTAC 
TCCAGGTACT TCGTGCTACA GAAACGTCGC GGCCACGGCG AGTCGCGCGG CTTTGGGTCC 
ATGCTGGTGG GCACCTACGA CAGTGTTTGG AACACCAAGC CGGCTCCGTA TCGCATTGTA 
CACCGGACAC CGTCCTCGGA AGTTTCTTAC GAAATCGCCA TTGGAATAAC GCAGGATGAG 
ATTGCAAAGG ACTGGGAATG GCTGCAGGCG AATCTCTTCA ATGTGCTCAA CGAAATGGAG 
AACGAGGACG AGGTTACAAA CTTTACTATA TGCAAAATCA AATCGCTCTA CACACAAAAT 
AATCAAGATG AAACTGGCGA AACGGCCGAC TTCAAGGTGA TGACCTCTCA GTTTAGACAA 
ACCTTCAAAA TGCCGGAGGA AGAGCGTCTG GTCAACTCCT ACTCGGCCAC ATATGTGAAG 
AACAAGATAC CGCGTCAGGG TCAGCTATAC ATCTCTTTGA ATCACGTCTG CTTCTACTCG 
TATATGCTGG GTCATGAGGT CAAGAGGATA ATACGCTTTG CCGAGCTGGA GGACATCAGT 
CGGAATGCAA ATACGATTTA CTTGAAGACC ACCAACAATA TGACATACAA TTTTACGCTG 
ATCTTCAATG CCGGCGAGGC ACATCTGCTG ATCGAGCAGC TCAACAAAAT GGCCATACAA 
CAGCTGATCC AGGATCCAGA GAGTCCAGTG GTCGATCATG ATCCGTCGAA CTTTGCCCGA 
CTGGGCGCTA AATCATCCAA GAAACCGGTT CTGCTCAGGG ATCTGACAGC CCGCCAGAAG 
TCCGAGGAGT TTCGCATTTA TTTCCGCTTA CCCCAAACGG AGATAATCGA TGGGAAGATC 
AAGGCAAACA TTTGGACACC CTACTCGAAG CGGTTTAATG CTGGCTACAT ATATTTGTCG 
CCCAATTTCT TTTGCTTTCG CAGCGATGTC AAGGACTTGG TTAGTGTGGT CATACCCATG 
AAAACAATAA AGAGTGTGGA GAAGAAGGAC GATGGGCAGC AGCGTTTTGA CAATCAAATT 
GTCATCATCA CGTCGGAGAA TGTGCCTTTC ATGTTTGCCC AGATCGTCGA TAGAGAGGTG 
CTGATCTCAA AAATAACAGA TCTACTCTCC CGCATTCATG TTCCCGTGAG TCGGGAACGT 
GCCAAGTACG ACATCTCCTG GAGCAAGCAG ACAGCCTTGA TGAACACATT CAAGACACAG 
TTCAGTTCAG ACATAATCAG GAATCAGGAG GAGAAGATGG TGCGTTGGGA GGCACATTTC 
CGTGACTTTG GACGCGGCAT TGGCATGTTC CGCACCACCG ATGTCATAAA TCTCATTGTG 
GAGGGCATTC CGGACCAGTT GCGTCAGGAG ATTTGGTTGA TCTTCTCCGG TGCCATTCAC 
GACAAGGAAA TGAATCCCGG ATTGTACGAG GATCTGGTGG AGAAGGCTGC CTGCATTAAG 
GATTGCTTTG CTCACGACGA AATCGACAGG GATCTGCCCC GTTCACTGCC CGAGCATCCG 
GCATTCCAGA GCACAGACGG CATTGGCGCA CTGAGGCGCG TCCTGCAAGC CTATGCCCTG 
CGCAATCCGC CCGTTAAGGG CTGCCAGGCC ATGAACATTG TCTCGTCGGG ATCCTTACTG 
TTTTGCGACG AAGAGAACGC CTTCTGGATG CTGGCCAGCC TGTGCGAGAA CCTCTTGCCG 
GACTACTACA AGGACAAGGT GGTGGGGGCA CAGATTGATC AGGGCGTGCT CAATGAACTG 
GTCGAAACTC ATCTGGCCGA TCTGCACGAG CATCTGGAGC AGCTGGGCGT CATCAAAATG 
ATCTCCATTT CGTGGTTCCT CACCATCTTC ATGAGCGTAA TCAGCTATGA GAGCTCGCTC 
CACATACTCG ACTGTTTCTT CTACGAGGGC GCCAAGATCA TCTTTATGGT GGCTTTGCAG 
ATCATCGAAT GGAATCGCGA CAAGCTGCTC AAGTGCCAGG ACGATGGCGA GGCCATGCTG 
GTGCTGCAAA ACTATCTGGA GGGCATTTAC AATCCCGACT ATCAAGTGCC GCCCTCCACG 
GACAAACGCA AGATGGAACG CTCACAGACG CAAACTGTGC AGACCCTCAT CCACGAGGCG 
TACACAAAAT TCGGCGAGGA ACTGACCCAG CAGCGCATCG AGGAGCTGCG CAACAAGCAC 
CGACGCCTGA CCATGCGTCA GTTTGACATT GACAACGAGA AGACCGTTGT CAAGGCCTAT 
TTACAGAATC CCTATTTCGA TCGAAACGAG CTGCACATGC TGCTCACCAT CATCCGAGAG 
GAAAAACACG CGTTGAAGTC CATGCAGCAG CAGCAGAAGG CACAATGCTC GCTGTCGGAG 
ACGCCGCAGC TGCTGCATCA TCAATCGCCC ACCAGGGTAC CGAATGACCC TGTCCTGGGT 
GGCAGTAATG GCAGATACGA GGCCTACTGG GTCAACTATG AGGTATTCCA CACACTCTTC 
ACAGAGCTAA CTCCCTGGCG GAAGTGTGTC AGCGTGGATC TTGGCGAAAA GTTGTTCAGG 
CTCACAGACA AGAAGGGTAC TGGATATCTG GATTTTGGAC AGCTTATCAA TGCCCTGGGC 
TTGGTTTGCT CCTCCAAGAG CATGGAGAAA CTGAAATTAC TTTATGTGTT GCATTTGCCG 
CCTCTGTTAT CCAAGGCGGA AATCGAACGA TCACGTCGTC CGCGTCCACG GACCAAAGAT 
GATGCCGAGG AGGCCTTTGA GGCGGAAGAT TTCTTTGACG ACGATGCTTC AGAGTCCATG 
GAGGCTTTGC CCTCGCCCTC GGATCATCAA TTTGATGCCG ATGACTATGC TCTGATCAGT 
GCCACACAGC ATTTGCATAA TCTGGCGGGT ATATCGGGCA ATACGTTCAT GGATCTATCA 
CGCAACACGC CCAATTTGTC GCTGAATTCC AACACCTCCT CGCTGGCGCA GCGCAATTCA 
ACATTCTATG TGGATCTGCC AGCAGCAGCA CTCCCGGATC TGGGAGCGGC ACGAATGACT 
CCGCCGCCGG GAGCGACTGC TGGAGCAGCC TCAGCTTCCG CGGCTGCTGC TGCTGCAGCC 
GATAAAATGC TTCTGAATGT CGAGACTATA TCGAATTTCT CACAGATCAG TGACCTGGTT 
GCTGCTACGC GACTGGAGCG TGCCGACTCG AATGCCACGG ATTCGAGAAG CCTGGGCAGC 
CTCGGCTATC TGCTCGACCA GCCCGATGAG GGGGCCTCGG GCTCGCATCA GACCATTCCG 
CATTTGCGCA AGGAGAACTT CCAGCTGCTG TGGCGCAGCA TTATCGAGAT CATGGGCTGT 
GTGCAGGACG AGGAGATGCG AATGGCATAT GAGAATCTGC TGGAGCTGGG AAACAGCAAC 
ATCAAAAGGA ACCCAGCCTA GAGAGGCTTT ACCCCAACTC AATTTGGCGG CAGCCGGCAA 
CCGGATAGCA ATGGCAACCC CACAACGCCC GCAGCAGAGC ACAGCAGCAC CACCCGTCTA 
TTTGTGGCAC TCGAGGAGGA GCTCCGTCAG GCCAGCGGGA AGCCCAGCAG CAGCAGGGGT 
AGCAGCAGTA GCAGCACAAA CACCTCCGAG GCCTGGCACA TATCCATCAA TCAATTTATA 
GCCACGGTTC TCACCGTGAA TACCATTGTA CAAGGTTTCC AAACACCCAT CCAAATAACC 
GAACAGGTCG AACAGCTCCA GAAGAAGCGA CGCAAATGTC TATCCACCAA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA23359 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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